Rroxscaffold_1G00039360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003282
Physical Location & Seq
Reverse (-)
56965122 .. 56967188
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039360.1

Sequence Viewer

Length: 1053 bp
ATGATGATGAGTTTAAAAGGGACGACTTCCTCCCCCACCGGACTTTGTTTTTGGTGCTTCCTCCTCTGCTTATCAGGTGGAAGGTGCTGCAGACCAAGACGGGAGAACCCCAAGCATCTTGGACACGCTTGCGCATGCTGCCGCCGCTTGCTAGGTTTCCAATGGCACTCCGTTGCGTCAATCCTAATCGGTGCTATTTCTTTGCCTCGCGAGGAGAAGAATGTATGCCGCCCTAAGAGCGTCGAGAATGCGCCTCCGCAGGCTGAACTCGTGCGCCGATCTTCAAAGGACGTGGTTGTTGTGAGTCTAACAACAAGTGACGTGCGGTGTAAATGGGGGACTACGGAGTACTCTTCTCAAGTGAACGTCGGTGGCCGGCGTTGGAAGTCGTGGGTGGTGGAGATGGGATCCTCTCTCAACAGACCAAGCAGAGGACGTCAGAAGAATTTGGATGATAGGCTTGCAAAGCTGAATGGGATCTACTCTTGCCGGTTGTTGGATGCGGCAAAGGATCATTTCCGTCCCCAAAAAAAGGTAGTGGCGATCTGGTATTCAAGCAATCCAGCAATTCGGCTTCGAATTGCACGAGCTGTGAAGTCGAGCGTTGGTCTTGCCATAGAGATGTTGCGTCCAGATGCAGCGAGAAGACCACAGAGGTGGCGTGTTGCAGCGATGCTTGGTGATGGTACTGATGCTGATCAGAAGGTGGTAGCAAGGTTGGAAGATGTGGTGCCCAGATTGCACTGGGCAGCGGCGACATTAACCACGAGTGCAGAAGAGGGCGGGCCAAAAGTGGGCTGGGTGATGAGATTGGATTTGTGGCCCAAGTCACGTTGGTGGCCCATTGCAATTTGGGTATTCAAATTAATGCCTTGGGTGTCCAGAGAGATGTGCAGGCCCAACTCTGTTTGGGTGCTAAATAGGTGTTGGGTGGCCGAAACAAATAGAGGTCTCTTTGGATTGGGCCTCTTTCATGTGACAAACTATTGGATTCAAATTCTATGGGGTTTCGTATTTGAGATTCTGGAGGATAGACCCAACCAAACCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

350

Amino Acids

39.81

Weight (kDa)

10.09

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 439
Acc16I TGCGCA 1 cut(s) 133
AccB1I GGYRCC 1 cut(s) 730
AccII CGCG 1 cut(s) 210
AciI CCGC 8 cut(s) 142, 145, 229, 257, 325, 503, 752, 783
AclWI GGATC 4 cut(s) 402, 415, 485, 519
AcoI YGGCCR 2 cut(s) 373, 933
AcsI RAATTY 2 cut(s) 445, 996
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 2 cut(s) 350, 688
AfiI CCNNNNNNNGG 4 cut(s) 431, 496, 532, 794
AgsI TTSAA 4 cut(s) 285, 555, 862, 995
AjiI CACGTC 2 cut(s) 292, 322
AleI CACNNNNGTG 2 cut(s) 655, 835
AluBI AGCT 2 cut(s) 469, 590
AluI AGCT 2 cut(s) 469, 590
Alw26I GTCTC 1 cut(s) 956
AlwI GGATC 4 cut(s) 402, 415, 485, 519
AoxI GGCC 7 cut(s) 373, 785, 821, 839, 896, 933, 964
ApeKI GCWGC 5 cut(s) 87, 138, 638, 668, 749
ApoI RAATTY 2 cut(s) 445, 996
ArsI GACNNNNNNTTYG 2 cut(s) 33, 65
AseI ATTAAT 1 cut(s) 866
AspLEI GCGC 3 cut(s) 134, 253, 276
AspS9I GGNCC 5 cut(s) 785, 822, 840, 897, 964
AsuHPI GGTGA 2 cut(s) 692, 814
AsuII TTCGAA 1 cut(s) 577
BaeGI GKGCMC 1 cut(s) 735
BamHI GGATCC 1 cut(s) 407
BanI GGYRCC 1 cut(s) 730
BauI CACGAG 3 cut(s) 269, 585, 766
BbsI GAAGAC 1 cut(s) 652
BbvI GCAGC 5 cut(s) 74, 125, 650, 680, 761
BccI CCATC 2 cut(s) 397, 677
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 2 cut(s) 152, 1051
BfmI CTRYAG 1 cut(s) 88
BmcAI AGTACT 1 cut(s) 350
BmgBI CACGTC 2 cut(s) 292, 322
BmgT120I GGNCC 5 cut(s) 785, 822, 840, 897, 964
BmiI GGNNCC 2 cut(s) 409, 732
BmrI ACTGGG 1 cut(s) 754
BmsI GCATC 5 cut(s) 124, 490, 625, 663, 682
BmuI ACTGGG 1 cut(s) 754
BpiI GAAGAC 1 cut(s) 652
BpmI CTGGAG 1 cut(s) 1046
Bpu14I TTCGAA 1 cut(s) 577
BpuEI CTTGAG 1 cut(s) 342
BsaBI GATNNNNATC 1 cut(s) 696
BsaHI GRCGYC 1 cut(s) 436
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 1 cut(s) 872
BsaWI WCCGGW 1 cut(s) 38
BsaXI ACNNNNNCTCC 1 cut(s) 1031
Bsc4I CCNNNNNNNGG 4 cut(s) 431, 496, 532, 794
Bse118I RCCGGY 2 cut(s) 375, 489
Bse1I ACTGG 1 cut(s) 749
Bse3DI GCAATG 1 cut(s) 843
Bse8I GATNNNNATC 1 cut(s) 696
BseDI CCNNGG 1 cut(s) 872
BseGI GGATG 2 cut(s) 457, 505
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 4 cut(s) 431, 496, 532, 794
BseMI GCAATG 1 cut(s) 843
BseNI ACTGG 1 cut(s) 749
BseRI GAGGAG 2 cut(s) 53, 227
BseSI GKGCMC 1 cut(s) 735
BseXI GCAGC 5 cut(s) 74, 125, 650, 680, 761
BseYI CCCAGC 1 cut(s) 798
BsgI GTGCAG 2 cut(s) 792, 913
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 7 cut(s) 375, 787, 823, 841, 898, 935, 966
BshNI GGYRCC 1 cut(s) 730
BsiSI CCGG 3 cut(s) 39, 376, 490
BslFI GGGAC 3 cut(s) 34, 352, 507
BslI CCNNNNNNNGG 4 cut(s) 431, 496, 532, 794
BsmAI GTCTC 1 cut(s) 956
BsmFI GGGAC 3 cut(s) 34, 352, 507
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 7 cut(s) 375, 787, 823, 841, 898, 935, 966
Bso31I GGTCTC 1 cut(s) 956
Bsp119I TTCGAA 1 cut(s) 577
Bsp1286I GDGCHC 1 cut(s) 735
Bsp143I GATC 6 cut(s) 278, 407, 477, 511, 543, 697
Bsp68I TCGCGA 1 cut(s) 210
BspACI CCGC 8 cut(s) 142, 145, 229, 257, 325, 503, 752, 783
BspANI GGCC 7 cut(s) 375, 787, 823, 841, 898, 935, 966
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 2 cut(s) 409, 732
BspMAI CTGCAG 1 cut(s) 92
BspPI GGATC 4 cut(s) 402, 415, 485, 519
BspT104I TTCGAA 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 843
BsrFI RCCGGY 2 cut(s) 375, 489
BsrI ACTGG 1 cut(s) 749
BssAI RCCGGY 2 cut(s) 375, 489
BssECI CCNNGG 1 cut(s) 872
BssMI GATC 6 cut(s) 278, 407, 477, 511, 543, 697
BssNI GRCGYC 1 cut(s) 436
BssSI CACGAG 3 cut(s) 269, 585, 766
BssT1I CCWWGG 1 cut(s) 872
Bst2BI CACGAG 3 cut(s) 269, 585, 766
Bst6I CTCTTC 2 cut(s) 358, 771
BstACI GRCGYC 1 cut(s) 436
BstBI TTCGAA 1 cut(s) 577
BstC8I GCNNGC 8 cut(s) 130, 136, 149, 261, 377, 462, 785, 896
BstDEI CTNAG 1 cut(s) 234
BstF5I GGATG 2 cut(s) 457, 505
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 3 cut(s) 134, 253, 276
BstKTI GATC 6 cut(s) 281, 410, 480, 514, 546, 700
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 6 cut(s) 278, 407, 477, 511, 543, 697
BstMWI GCNNNNNNNGC 5 cut(s) 138, 144, 237, 466, 739
BstNSI RCATGY 1 cut(s) 138
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 735
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 5 cut(s) 74, 125, 650, 680, 761
BstV2I GAAGAC 1 cut(s) 652
BstX2I RGATCY 2 cut(s) 407, 477
BstXI CCANNNNNNTGG 1 cut(s) 657
BstYI RGATCY 2 cut(s) 407, 477
BsuRI GGCC 7 cut(s) 375, 787, 823, 841, 898, 935, 966
BtgZI GCGATG 1 cut(s) 686
BtrI CACGTC 2 cut(s) 292, 322
BtsCI GGATG 2 cut(s) 457, 505
BtsIMutI CAGTG 1 cut(s) 742
BtuMI TCGCGA 1 cut(s) 210
Cac8I GCNNGC 8 cut(s) 130, 136, 149, 261, 377, 462, 785, 896
CfoI GCGC 3 cut(s) 134, 253, 276
Cfr10I RCCGGY 2 cut(s) 375, 489
Cfr13I GGNCC 5 cut(s) 785, 822, 840, 897, 964
CseI GACGC 3 cut(s) 165, 229, 617
Csp6I GTAC 2 cut(s) 349, 687
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 2 cut(s) 135, 974
CviQI GTAC 2 cut(s) 349, 687
DdeI CTNAG 1 cut(s) 234
DpnI GATC 6 cut(s) 280, 409, 479, 513, 545, 699
DpnII GATC 6 cut(s) 278, 407, 477, 511, 543, 697
DraI TTTAAA 1 cut(s) 15
EaeI YGGCCR 2 cut(s) 373, 933
Eam1104I CTCTTC 2 cut(s) 358, 771
EarI CTCTTC 2 cut(s) 358, 771
Eco130I CCWWGG 1 cut(s) 872
Eco31I GGTCTC 1 cut(s) 956
EcoT14I CCWWGG 1 cut(s) 872
ErhI CCWWGG 1 cut(s) 872
FaeI CATG 2 cut(s) 138, 977
FaiI YATR 5 cut(s) 136, 226, 617, 975, 1003
FaqI GGGAC 3 cut(s) 34, 352, 507
FatI CATG 2 cut(s) 134, 973
FauI CCCGC 1 cut(s) 776
FbaI TGATCA 1 cut(s) 697
FokI GGATG 2 cut(s) 464, 512
FspBI CTAG 2 cut(s) 152, 1051
FspI TGCGCA 1 cut(s) 133
GlaI GCGC 3 cut(s) 133, 252, 275
GsaI CCCAGC 1 cut(s) 802
GsuI CTGGAG 1 cut(s) 1046
HaeIII GGCC 7 cut(s) 375, 787, 823, 841, 898, 935, 966
HapII CCGG 3 cut(s) 39, 376, 490
HgaI GACGC 3 cut(s) 165, 229, 617
HhaI GCGC 3 cut(s) 134, 253, 276
Hin1I GRCGYC 1 cut(s) 436
Hin1II CATG 2 cut(s) 138, 977
Hin6I GCGC 3 cut(s) 132, 251, 274
HinP1I GCGC 3 cut(s) 132, 251, 274
HinfI GANTC 3 cut(s) 304, 991, 1021
HpaII CCGG 3 cut(s) 39, 376, 490
HphI GGTGA 2 cut(s) 692, 814
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 441, 702
Hpy188III TCNNGA 5 cut(s) 209, 244, 632, 882, 1025
Hpy8I GTNNAC 1 cut(s) 364
Hpy99I CGWCG 2 cut(s) 245, 371
HpyAV CCTTC 2 cut(s) 75, 697
HpyCH4IV ACGT 5 cut(s) 291, 321, 366, 436, 832
HpyCH4V TGCA 9 cut(s) 90, 464, 584, 638, 668, 742, 773, 848, 894
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 144, 237, 466, 739
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 5 cut(s) 291, 321, 366, 436, 832
Hsp92I GRCGYC 1 cut(s) 436
Hsp92II CATG 2 cut(s) 138, 977
HspAI GCGC 3 cut(s) 132, 251, 274
KroI GCCGGC 1 cut(s) 375
KroNI GCCGGC 1 cut(s) 377
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 6 cut(s) 278, 407, 477, 511, 543, 697
Lsp1109I GCAGC 5 cut(s) 74, 125, 650, 680, 761
LweI GCATC 5 cut(s) 124, 490, 625, 663, 682
MaeI CTAG 2 cut(s) 152, 1051
MaeII ACGT 5 cut(s) 291, 321, 366, 436, 832
MaeIII GTNAC 3 cut(s) 317, 828, 976
MalI GATC 6 cut(s) 280, 409, 479, 513, 545, 699
MboI GATC 6 cut(s) 278, 407, 477, 511, 543, 697
MboII GAAGA 7 cut(s) 229, 273, 345, 454, 657, 734, 788
MflI RGATCY 2 cut(s) 407, 477
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 6 cut(s) 445, 567, 579, 849, 863, 996
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 3 cut(s) 362, 477, 699
MroNI GCCGGC 1 cut(s) 375
MseI TTAA 3 cut(s) 14, 761, 866
MslI CAYNNNNRTG 3 cut(s) 620, 655, 835
MspA1I CMGCKG 1 cut(s) 752
MspI CCGG 3 cut(s) 39, 376, 490
Mva1269I GAATGC 1 cut(s) 253
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 5 cut(s) 138, 144, 237, 466, 739
NaeI GCCGGC 1 cut(s) 377
NdeII GATC 6 cut(s) 278, 407, 477, 511, 543, 697
NgoMIV GCCGGC 1 cut(s) 375
NlaIII CATG 2 cut(s) 138, 977
NlaIV GGNNCC 2 cut(s) 409, 732
NmuCI GTSAC 3 cut(s) 317, 828, 976
NruI TCGCGA 1 cut(s) 210
NsbI TGCGCA 1 cut(s) 133
NspI RCATGY 1 cut(s) 138
NspV TTCGAA 1 cut(s) 577
OliI CACNNNNGTG 2 cut(s) 655, 835
PaeI GCATGC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 1 cut(s) 583
PctI GAATGC 1 cut(s) 253
PdiI GCCGGC 1 cut(s) 377
PfeI GAWTC 2 cut(s) 991, 1021
PleI GAGTC 1 cut(s) 312
PpsI GAGTC 1 cut(s) 312
PshBI ATTAAT 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 798
PspN4I GGNNCC 2 cut(s) 409, 732
PspPI GGNCC 5 cut(s) 785, 822, 840, 897, 964
PstI CTGCAG 1 cut(s) 92
PsuI RGATCY 2 cut(s) 407, 477
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 2 cut(s) 350, 688
RsaNI GTAC 2 cut(s) 349, 687
RseI CAYNNNNRTG 3 cut(s) 620, 655, 835
SaqAI TTAA 3 cut(s) 14, 761, 866
Sau3AI GATC 6 cut(s) 278, 407, 477, 511, 543, 697
Sau96I GGNCC 5 cut(s) 785, 822, 840, 897, 964
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 1 cut(s) 313
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 5 cut(s) 124, 490, 625, 663, 682
SfcI CTRYAG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 577
SmiMI CAYNNNNRTG 3 cut(s) 620, 655, 835
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
SphI GCATGC 1 cut(s) 138
Sse9I AATT 6 cut(s) 445, 567, 579, 849, 863, 996
SsiI CCGC 8 cut(s) 142, 145, 229, 257, 325, 503, 752, 783
SspMI CTAG 2 cut(s) 152, 1051
StyI CCWWGG 1 cut(s) 872
TaiI ACGT 5 cut(s) 294, 324, 369, 439, 835
TaqI TCGA 3 cut(s) 243, 577, 599
TasI AATT 6 cut(s) 445, 567, 579, 849, 863, 996
TatI WGTACW 1 cut(s) 348
TauI GCSGC 5 cut(s) 144, 147, 231, 506, 755
TfiI GAWTC 2 cut(s) 991, 1021
Tru1I TTAA 3 cut(s) 14, 761, 866
Tru9I TTAA 3 cut(s) 14, 761, 866
TscAI CASTG 1 cut(s) 749
TseFI GTSAC 3 cut(s) 317, 828, 976
TseI GCWGC 5 cut(s) 87, 138, 638, 668, 749
Tsp45I GTSAC 3 cut(s) 317, 828, 976
TspDTI ATGAA 1 cut(s) 962
TspGWI ACGGA 3 cut(s) 160, 359, 509
TspRI CASTG 1 cut(s) 749
VspI ATTAAT 1 cut(s) 866
XapI RAATTY 2 cut(s) 445, 996
XceI RCATGY 1 cut(s) 138
XcmI CCANNNNNNNNNTGG 1 cut(s) 795
XspI CTAG 2 cut(s) 152, 1051
ZraI GACGTC 1 cut(s) 437
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.