Rorug03G0305200

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0003282
Physical Location & Seq
Forward (+)
32376721 .. 32380929
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0305200.1

Sequence Viewer

Length: 1236 bp
ATGGCGTTCGAGAAGATCAAGGTCGCTAACCCCATCGTCGAGATGGACGGAGATGAGATGACTCGAATTTTCTGGAAATCAATCAAGGATAAGCTTATTCTCCCCTTTTTGGATTTGGACATTAAGTACTTTGACCTTGGTCTTCCTCACCGTGACGCCACTGATGATAAAGTTACCGTTGAAAGTGCAGAGGCTACTCTTAAGTACAATGTAGCAATCAAATGCGCGACTATCACACCAGATGAAGCTCGCATGAAGGAGTTTACCTTGAAGCAAATGTGGAGGAGTCCCAATGGGACTATTAGGAATATTTTGAATGGTACTGTTTTCAGAGAACCGATTCTTTGCAAGAACATTCCTCGCCTAATCCCAGGTTGGACGAAGCCAATATGCATTGGAAGACATGCTTTCGGTGATCAATATCGAGCAACTGATACAGTTATCAAAGGACCTGGAAAACTGAAATTGCTGTTTGTTCCAGAAGGAAAGGATGAGAAGACTGAACTGGAAGTGTACAACTTTACAGGGGAAGGCGGAGTAGCAATTGCCATGTACAACACCGATGAGTCAATCCGTGCTTTTGCAGAAGCTTCTATGAACACAGCTTATGAGAAAAAGTGGCCTCTTTATCTTAGCACAAAAAACACTATTCTTAAGAAGTATGATGGAAGATTCAAGGACATATTTCAAGAAGTTTATGAAGCTCATTGGAAATCCAAGTATGAAGCTGCTGGCATATGGTATGAACATCGTCTCATTGATGATATGGTGGCTTATGCACTTAAAAGTGAAGGTGGTTATGTTTGGGCATGCAAGAATTATGATGGAGATGTGCAAAGTGATTTCTTAGCTCAAGGTTTTGGATCTCTTGGATTGATGACATCAGTACTGGTGTGCCCAGATGGAAAGACCATAGAAGCTGAAGCTGCTCATGGTACAGTTACTCGGCATTATAGAGTTCACCAGAAAGGTGGTGAAACCAGTACAAACAGCATAGCTTCAATTTTTGCTTGGACAAGAGGGCTTGCACACAGGGCAAAGTTGGATGACAATGCTAAATTGTTGGATTTCACTCAGAAGCTGGAAGAAGCTTGTATTGGAACTGTGGAATCTGGGAAAATGACCAAGGATCTTGCACTAATTCTTCATGGACCTAAGCTTGCTAGGAACCACTACTTGAACACGGAGGAGTTCATTGATGCCGTGGCTGCAGAACTGAAAGCAAAGCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003682 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006464 GO:0006479 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006996 GO:0008150 GO:0008152 GO:0008213 GO:0009605 GO:0009607 GO:0009615 GO:0009617 GO:0009814 GO:0009816 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015030 GO:0016032 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0016569 GO:0016570 GO:0016571 GO:0016604 GO:0016787 GO:0016788 GO:0018022 GO:0018193 GO:0018205 GO:0019048 GO:0019219 GO:0019222 GO:0019538 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0034641 GO:0034968 GO:0035194 GO:0035197 GO:0035198 GO:0035821 GO:0036211 GO:0040029 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0043412 GO:0043414 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045087 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051567 GO:0051607 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0060255 GO:0061647 GO:0061980 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0080188 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0097159 GO:0098542 GO:0140098 GO:1901360 GO:1901363 GO:1901564 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

411

Amino Acids

46.3

Weight (kDa)

6.58

Isoelectric Point (pI)

19.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Iso_dh PF00180 7 - 402 1.4e-83 Isocitrate/isopropylmalate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 227
AciI CCGC 1 cut(s) 534
AclWI GGATC 2 cut(s) 871, 1137
AcsI RAATTY 1 cut(s) 66
AcuI CTGAAG 1 cut(s) 942
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 8 cut(s) 128, 206, 322, 515, 554, 888, 937, 985
AfiI CCNNNNNNNGG 1 cut(s) 109
AflII CTTAAG 2 cut(s) 200, 653
AgsI TTSAA 7 cut(s) 182, 271, 316, 676, 689, 1002, 1180
AjnI CCWGG 2 cut(s) 370, 451
AjuI GAANNNNNNNTTGG 2 cut(s) 1080, 1112
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 2 cut(s) 871, 1137
AlwNI CAGNNNCTG 1 cut(s) 1081
AoxI GGCC 1 cut(s) 620
ApeKI GCWGC 3 cut(s) 728, 926, 1208
ApoI RAATTY 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 1 cut(s) 227
AspS9I GGNCC 2 cut(s) 449, 1151
AsuHPI GGTGA 4 cut(s) 140, 425, 953, 986
AvaII GGWCC 2 cut(s) 449, 1151
BaeGI GKGCMC 1 cut(s) 899
BbsI GAAGAC 3 cut(s) 134, 406, 503
BbvI GCAGC 3 cut(s) 715, 913, 1195
BccI CCATC 5 cut(s) 37, 41, 659, 818, 896
BceAI ACGGC 1 cut(s) 1187
BciT130I CCWGG 2 cut(s) 372, 453
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 758
BfaI CTAG 1 cut(s) 1164
BfmI CTRYAG 1 cut(s) 1209
BfrI CTTAAG 2 cut(s) 200, 653
BisI GCNGC 3 cut(s) 729, 927, 1209
BlsI GCNGC 3 cut(s) 730, 928, 1210
BmcAI AGTACT 2 cut(s) 128, 888
Bme1390I CCNGG 2 cut(s) 372, 453
Bme18I GGWCC 2 cut(s) 449, 1151
BmgT120I GGNCC 2 cut(s) 449, 1151
BmiI GGNNCC 1 cut(s) 1169
BmrFI CCNGG 2 cut(s) 372, 453
BmsI GCATC 1 cut(s) 1189
BoxI GACNNNNGTC 1 cut(s) 138
BpiI GAAGAC 3 cut(s) 134, 406, 503
Bpu10I CCTNAGC 1 cut(s) 1155
BpuEI CTTGAG 1 cut(s) 837
BsaBI GATNNNNATC 1 cut(s) 420
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 4 cut(s) 136, 370, 1125, 1203
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse1I ACTGG 3 cut(s) 510, 894, 981
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 2 cut(s) 372, 453
BseDI CCNNGG 4 cut(s) 136, 370, 1125, 1203
BseGI GGATG 2 cut(s) 496, 1051
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 1088
BseNI ACTGG 3 cut(s) 510, 894, 981
BseRI GAGGAG 2 cut(s) 298, 1202
BseSI GKGCMC 1 cut(s) 899
BseXI GCAGC 3 cut(s) 715, 913, 1195
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 1 cut(s) 622
BslFI GGGAC 2 cut(s) 273, 310
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 1 cut(s) 758
BsmBI CGTCTC 1 cut(s) 758
BsmFI GGGAC 2 cut(s) 273, 310
BsnI GGCC 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 899
Bsp1407I TGTACA 2 cut(s) 513, 552
Bsp143I GATC 4 cut(s) 15, 415, 863, 1129
BspACI CCGC 1 cut(s) 534
BspANI GGCC 1 cut(s) 622
BspCNI CTCAG 1 cut(s) 1087
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 1 cut(s) 1169
BspMAI CTGCAG 1 cut(s) 1213
BspPI GGATC 2 cut(s) 871, 1137
BspTI CTTAAG 2 cut(s) 200, 653
BsrGI TGTACA 2 cut(s) 513, 552
BsrI ACTGG 3 cut(s) 510, 894, 981
BssECI CCNNGG 4 cut(s) 136, 370, 1125, 1203
BssMI GATC 4 cut(s) 15, 415, 863, 1129
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 136, 1125
Bst2UI CCWGG 2 cut(s) 372, 453
Bst4CI ACNGT 6 cut(s) 152, 178, 325, 439, 940, 1105
BstACI GRCGYC 1 cut(s) 156
BstAFI CTTAAG 2 cut(s) 200, 653
BstAUI TGTACA 2 cut(s) 513, 552
BstC8I GCNNGC 5 cut(s) 250, 733, 811, 1026, 1161
BstDEI CTNAG 4 cut(s) 632, 847, 1074, 1155
BstDSI CCRYGG 1 cut(s) 1203
BstF5I GGATG 2 cut(s) 496, 1051
BstFNI CGCG 1 cut(s) 227
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 4 cut(s) 18, 418, 866, 1132
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 4 cut(s) 15, 415, 863, 1129
BstMWI GCNNNNNNNGC 3 cut(s) 926, 1034, 1208
BstNI CCWGG 2 cut(s) 372, 453
BstNSI RCATGY 2 cut(s) 407, 813
BstPAI GACNNNNGTC 1 cut(s) 138
BstSCI CCNGG 2 cut(s) 370, 451
BstSFI CTRYAG 1 cut(s) 1209
BstSLI GKGCMC 1 cut(s) 899
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 3 cut(s) 715, 913, 1195
BstV2I GAAGAC 3 cut(s) 134, 406, 503
BstX2I RGATCY 2 cut(s) 863, 1129
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 2 cut(s) 863, 1129
BsuRI GGCC 1 cut(s) 622
BtgI CCRYGG 1 cut(s) 1203
BtsCI GGATG 2 cut(s) 496, 1051
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 5 cut(s) 250, 733, 811, 1026, 1161
CaiI CAGNNNCTG 1 cut(s) 1081
CfoI GCGC 1 cut(s) 227
Cfr13I GGNCC 2 cut(s) 449, 1151
CseI GACGC 1 cut(s) 164
Csp6I GTAC 8 cut(s) 127, 205, 321, 514, 553, 887, 936, 984
CviAII CATG 6 cut(s) 253, 404, 550, 810, 932, 1148
CviQI GTAC 8 cut(s) 127, 205, 321, 514, 553, 887, 936, 984
DdeI CTNAG 4 cut(s) 632, 847, 1074, 1155
DpnI GATC 4 cut(s) 17, 417, 865, 1131
DpnII GATC 4 cut(s) 15, 415, 863, 1129
EciI GGCGGA 1 cut(s) 549
Eco130I CCWWGG 2 cut(s) 136, 1125
Eco47I GGWCC 2 cut(s) 449, 1151
Eco57I CTGAAG 1 cut(s) 942
EcoO109I RGGNCCY 1 cut(s) 449
EcoRII CCWGG 2 cut(s) 370, 451
EcoT14I CCWWGG 2 cut(s) 136, 1125
EcoT22I ATGCAT 1 cut(s) 395
ErhI CCWWGG 2 cut(s) 136, 1125
Esp3I CGTCTC 1 cut(s) 758
FaeI CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
FalI AAGNNNNNCTT 3 cut(s) 391, 423, 1212
FaqI GGGAC 2 cut(s) 273, 310
FatI CATG 6 cut(s) 252, 403, 549, 809, 931, 1147
FauNDI CATATG 1 cut(s) 737
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 3 cut(s) 729, 927, 1209
FokI GGATG 2 cut(s) 503, 1058
Fsp4HI GCNGC 3 cut(s) 729, 927, 1209
FspBI CTAG 1 cut(s) 1164
GlaI GCGC 1 cut(s) 226
GluI GCNGC 3 cut(s) 729, 927, 1209
HaeIII GGCC 1 cut(s) 622
HgaI GACGC 1 cut(s) 164
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HindIII AAGCTT 5 cut(s) 92, 588, 1089, 1157, 1226
HinfI GANTC 6 cut(s) 61, 286, 340, 566, 672, 1109
HphI GGTGA 4 cut(s) 140, 425, 953, 986
Hpy166II GTNNAC 3 cut(s) 264, 514, 961
Hpy188I TCNGA 2 cut(s) 332, 1077
Hpy188III TCNNGA 6 cut(s) 10, 40, 73, 479, 689, 1233
Hpy8I GTNNAC 3 cut(s) 264, 514, 961
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 250, 476, 524, 785
HpyCH4III ACNGT 6 cut(s) 152, 178, 325, 439, 940, 1105
HpyF10VI GCNNNNNNNGC 3 cut(s) 926, 1034, 1208
HpyF3I CTNAG 4 cut(s) 632, 847, 1074, 1155
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
HspAI GCGC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 4 cut(s) 15, 415, 863, 1129
Lsp1109I GCAGC 3 cut(s) 715, 913, 1195
LweI GCATC 1 cut(s) 1189
MaeI CTAG 1 cut(s) 1164
MaeIII GTNAC 3 cut(s) 152, 172, 940
MalI GATC 4 cut(s) 17, 417, 865, 1131
MboI GATC 4 cut(s) 15, 415, 863, 1129
MboII GAAGA 7 cut(s) 25, 134, 411, 508, 681, 1097, 1136
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 2 cut(s) 863, 1129
MhlI GDGCHC 1 cut(s) 899
MluCI AATT 7 cut(s) 66, 464, 543, 817, 1002, 1058, 1140
MlyI GAGTC 3 cut(s) 55, 295, 575
MmeI TCCRAC 3 cut(s) 356, 1023, 1044
MnlI CCTC 7 cut(s) 156, 184, 276, 369, 633, 1013, 1180
Mph1103I ATGCAT 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 339
MseI TTAA 4 cut(s) 123, 201, 654, 783
MspCI CTTAAG 2 cut(s) 200, 653
MspR9I CCNGG 2 cut(s) 372, 453
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 2 cut(s) 372, 453
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 3 cut(s) 926, 1034, 1208
NdeI CATATG 1 cut(s) 737
NdeII GATC 4 cut(s) 15, 415, 863, 1129
NlaIII CATG 6 cut(s) 256, 407, 553, 813, 935, 1151
NlaIV GGNNCC 1 cut(s) 1169
NmeAIII GCCGAG 1 cut(s) 925
NmuCI GTSAC 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 395
NspI RCATGY 2 cut(s) 407, 813
PaeI GCATGC 1 cut(s) 813
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 3 cut(s) 340, 672, 1109
PkrI GCNGC 3 cut(s) 730, 928, 1210
PleI GAGTC 3 cut(s) 55, 294, 574
PpsI GAGTC 3 cut(s) 55, 294, 574
PpuMI RGGWCCY 1 cut(s) 449
PshAI GACNNNNGTC 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 2 cut(s) 370, 451
PspGI CCWGG 2 cut(s) 370, 451
PspN4I GGNNCC 1 cut(s) 1169
PspPI GGNCC 2 cut(s) 449, 1151
PspPPI RGGWCCY 1 cut(s) 449
PstI CTGCAG 1 cut(s) 1213
PstNI CAGNNNCTG 1 cut(s) 1081
PsuI RGATCY 2 cut(s) 863, 1129
RsaI GTAC 8 cut(s) 128, 206, 322, 515, 554, 888, 937, 985
RsaNI GTAC 8 cut(s) 127, 205, 321, 514, 553, 887, 936, 984
SaqAI TTAA 4 cut(s) 123, 201, 654, 783
SatI GCNGC 3 cut(s) 729, 927, 1209
Sau3AI GATC 4 cut(s) 15, 415, 863, 1129
Sau96I GGNCC 2 cut(s) 449, 1151
ScaI AGTACT 2 cut(s) 128, 888
SchI GAGTC 3 cut(s) 55, 295, 575
ScrFI CCNGG 2 cut(s) 372, 453
SduI GDGCHC 1 cut(s) 899
SfaNI GCATC 1 cut(s) 1189
SfcI CTRYAG 1 cut(s) 1209
SinI GGWCC 2 cut(s) 449, 1151
SmlI CTYRAG 3 cut(s) 200, 653, 852
SmoI CTYRAG 3 cut(s) 200, 653, 852
SphI GCATGC 1 cut(s) 813
Sse9I AATT 7 cut(s) 66, 464, 543, 817, 1002, 1058, 1140
SsiI CCGC 1 cut(s) 534
SspI AATATT 1 cut(s) 310
SspMI CTAG 1 cut(s) 1164
StyD4I CCNGG 2 cut(s) 370, 451
StyI CCWWGG 2 cut(s) 136, 1125
TaaI ACNGT 6 cut(s) 152, 178, 325, 439, 940, 1105
TaqI TCGA 4 cut(s) 9, 39, 64, 424
TasI AATT 7 cut(s) 66, 464, 543, 817, 1002, 1058, 1140
TatI WGTACW 6 cut(s) 126, 204, 513, 552, 886, 983
TfiI GAWTC 3 cut(s) 340, 672, 1109
Tru1I TTAA 4 cut(s) 123, 201, 654, 783
Tru9I TTAA 4 cut(s) 123, 201, 654, 783
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 3 cut(s) 728, 926, 1208
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 8 cut(s) 258, 269, 611, 714, 738, 759, 1136, 1183
TspGWI ACGGA 3 cut(s) 63, 563, 1199
TspRI CASTG 1 cut(s) 166
Vha464I CTTAAG 2 cut(s) 200, 653
VpaK11BI GGWCC 2 cut(s) 449, 1151
XapI RAATTY 1 cut(s) 66
XceI RCATGY 2 cut(s) 407, 813
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 1 cut(s) 1164
ZrmI AGTACT 2 cut(s) 128, 888
Zsp2I ATGCAT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.