Rh4BG028000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003282
Physical Location & Seq
Reverse (-)
5043921 .. 5044373
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG028000.1

Sequence Viewer

Length: 453 bp
ATGGACGTCGCTGTTGTGCGTCCAACAACAAGTGACGTGCGATGTAAATGGGGGACTATGAAATACTCTTCTCATGTGAATGTCGGTGGCCGGCGTTGGCAGTCGTGGGTGATGGAGATGAGATCCTCTGTCGATCGACCACACAGAGGACGTCTGAAGAATCTGGATGATAGGCTTGCAAAGCTGAATGGGATCTGCTCTGGCCGGTTGTGGGATGTGGTAAAGGATTGCTTTCATCCCCAAAACAAGGTGGTGGCAATCTGGTATTCAAGCAATGCAGCAATTCGGCTTCGAATTGCAGGAGCTGTGAAATCGAACTTTGGTCTTGCCATAGAGTTGCGTTCAGATGTAGCGAAAAGACCATGGCGGGTTGTGGCGATGCTGGGTGATGGTACTAATGCTGATCAGAAGGTGGTAGCAACATTGGAGGATGTAGTGCCCAGAATGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.94

Weight (kDa)

10.23

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 9, 154
AciI CCGC 1 cut(s) 367
AclWI GGATC 2 cut(s) 117, 200
AcoI YGGCCR 2 cut(s) 88, 202
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 6, 151
AfaI GTAC 1 cut(s) 394
AfiI CCNNNNNNNGG 3 cut(s) 146, 211, 247
AgsI TTSAA 1 cut(s) 270
AjiI CACGTC 1 cut(s) 37
AluBI AGCT 2 cut(s) 184, 305
AluI AGCT 2 cut(s) 184, 305
AlwI GGATC 2 cut(s) 117, 200
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 2 cut(s) 88, 202
ApeKI GCWGC 1 cut(s) 278
AsuHPI GGTGA 2 cut(s) 121, 398
AsuII TTCGAA 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 290
BccI CCATC 2 cut(s) 106, 383
BclI TGATCA 1 cut(s) 403
BfaI CTAG 1 cut(s) 451
BisI GCNGC 1 cut(s) 279
BlsI GCNGC 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 37
BmsI GCATC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 292
BsaHI GRCGYC 2 cut(s) 6, 151
BsaJI CCNNGG 1 cut(s) 362
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 211, 247
Bse118I RCCGGY 2 cut(s) 90, 204
Bse3DI GCAATG 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 362
BseGI GGATG 4 cut(s) 172, 220, 235, 436
BseLI CCNNNNNNNGG 3 cut(s) 146, 211, 247
BseMI GCAATG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 1 cut(s) 290
BseYI CCCAGC 1 cut(s) 382
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 2 cut(s) 90, 204
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 2 cut(s) 91, 205
BslFI GGGAC 1 cut(s) 67
BslI CCNNNNNNNGG 3 cut(s) 146, 211, 247
BsmFI GGGAC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 2 cut(s) 90, 204
Bsp119I TTCGAA 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 4 cut(s) 122, 133, 192, 403
Bsp19I CCATGG 1 cut(s) 362
BspACI CCGC 1 cut(s) 367
BspANI GGCC 2 cut(s) 90, 204
BspPI GGATC 2 cut(s) 117, 200
BspT104I TTCGAA 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 280
BsrFI RCCGGY 2 cut(s) 90, 204
BssAI RCCGGY 2 cut(s) 90, 204
BssECI CCNNGG 1 cut(s) 362
BssMI GATC 4 cut(s) 122, 133, 192, 403
BssNI GRCGYC 2 cut(s) 6, 151
BssT1I CCWWGG 1 cut(s) 362
Bst6I CTCTTC 1 cut(s) 73
BstACI GRCGYC 2 cut(s) 6, 151
BstBI TTCGAA 1 cut(s) 292
BstC8I GCNNGC 2 cut(s) 92, 177
BstDSI CCRYGG 1 cut(s) 362
BstF5I GGATG 4 cut(s) 172, 220, 235, 436
BstKTI GATC 4 cut(s) 125, 136, 195, 406
BstMBI GATC 4 cut(s) 122, 133, 192, 403
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 2 cut(s) 181, 445
BstSLI GKGCMC 1 cut(s) 441
BstV1I GCAGC 1 cut(s) 290
BstX2I RGATCY 2 cut(s) 122, 192
BstYI RGATCY 2 cut(s) 122, 192
BsuRI GGCC 2 cut(s) 90, 204
BtgI CCRYGG 1 cut(s) 362
BtgZI GCGATG 2 cut(s) 55, 392
BtrI CACGTC 1 cut(s) 37
BtsCI GGATG 4 cut(s) 172, 220, 235, 436
Cac8I GCNNGC 2 cut(s) 92, 177
CaiI CAGNNNCTG 1 cut(s) 305
Cfr10I RCCGGY 2 cut(s) 90, 204
CseI GACGC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 393
CviAII CATG 2 cut(s) 74, 363
CviJI RGCY 6 cut(s) 90, 175, 184, 204, 289, 305
CviKI_1 RGCY 6 cut(s) 90, 175, 184, 204, 289, 305
CviQI GTAC 1 cut(s) 393
DpnI GATC 4 cut(s) 124, 135, 194, 405
DpnII GATC 4 cut(s) 122, 133, 192, 403
EaeI YGGCCR 2 cut(s) 88, 202
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 362
Eco57I CTGAAG 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 2 cut(s) 77, 366
FaiI YATR 4 cut(s) 59, 75, 332, 364
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 1 cut(s) 67
FatI CATG 2 cut(s) 73, 362
FauI CCCGC 1 cut(s) 360
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 1 cut(s) 279
FokI GGATG 4 cut(s) 179, 222, 227, 443
Fsp4HI GCNGC 1 cut(s) 279
FspBI CTAG 1 cut(s) 451
GluI GCNGC 1 cut(s) 279
GsaI CCCAGC 1 cut(s) 386
HaeIII GGCC 2 cut(s) 90, 204
HapII CCGG 2 cut(s) 91, 205
HgaI GACGC 1 cut(s) 8
Hin1I GRCGYC 2 cut(s) 6, 151
Hin1II CATG 2 cut(s) 77, 366
HinfI GANTC 1 cut(s) 160
HpaII CCGG 2 cut(s) 91, 205
HphI GGTGA 2 cut(s) 121, 398
Hpy188I TCNGA 3 cut(s) 156, 346, 408
Hpy188III TCNNGA 1 cut(s) 164
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 1 cut(s) 403
HpyCH4IV ACGT 3 cut(s) 6, 36, 151
HpyCH4V TGCA 4 cut(s) 179, 278, 299, 448
HpyF10VI GCNNNNNNNGC 2 cut(s) 181, 445
HpySE526I ACGT 3 cut(s) 6, 36, 151
Hsp92I GRCGYC 2 cut(s) 6, 151
Hsp92II CATG 2 cut(s) 77, 366
KroI GCCGGC 1 cut(s) 90
KroNI GCCGGC 1 cut(s) 92
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 4 cut(s) 122, 133, 192, 403
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 7 cut(s) 104, 149, 186, 218, 247, 285, 368
Lsp1109I GCAGC 1 cut(s) 290
LweI GCATC 1 cut(s) 369
MaeI CTAG 1 cut(s) 451
MaeII ACGT 3 cut(s) 6, 36, 151
MaeIII GTNAC 1 cut(s) 32
MalI GATC 4 cut(s) 124, 135, 194, 405
MboI GATC 4 cut(s) 122, 133, 192, 403
MboII GAAGA 2 cut(s) 60, 169
MflI RGATCY 2 cut(s) 122, 192
MhlI GDGCHC 1 cut(s) 441
MluCI AATT 2 cut(s) 282, 294
MmeI TCCRAC 1 cut(s) 47
MnlI CCTC 3 cut(s) 136, 140, 421
MroNI GCCGGC 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 2 cut(s) 91, 205
Mva1269I GAATGC 1 cut(s) 450
MwoI GCNNNNNNNGC 2 cut(s) 181, 445
NaeI GCCGGC 1 cut(s) 92
NcoI CCATGG 1 cut(s) 362
NdeII GATC 4 cut(s) 122, 133, 192, 403
NgoMIV GCCGGC 1 cut(s) 90
NlaIII CATG 2 cut(s) 77, 366
NmuCI GTSAC 1 cut(s) 32
NspV TTCGAA 1 cut(s) 292
PctI GAATGC 1 cut(s) 450
PdiI GCCGGC 1 cut(s) 92
PfeI GAWTC 1 cut(s) 160
PkrI GCNGC 1 cut(s) 280
Ple19I CGATCG 1 cut(s) 136
PspFI CCCAGC 1 cut(s) 382
PstNI CAGNNNCTG 1 cut(s) 305
PsuI RGATCY 2 cut(s) 122, 192
PvuI CGATCG 1 cut(s) 136
RsaI GTAC 1 cut(s) 394
RsaNI GTAC 1 cut(s) 393
RseI CAYNNNNRTG 1 cut(s) 78
SatI GCNGC 1 cut(s) 279
Sau3AI GATC 4 cut(s) 122, 133, 192, 403
SduI GDGCHC 1 cut(s) 441
SetI ASST 7 cut(s) 9, 39, 154, 186, 252, 307, 414
SfaNI GCATC 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 2 cut(s) 282, 294
SsiI CCGC 1 cut(s) 367
SspMI CTAG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 362
TaiI ACGT 3 cut(s) 9, 39, 154
TaqI TCGA 4 cut(s) 132, 136, 292, 314
TasI AATT 2 cut(s) 282, 294
TfiI GAWTC 1 cut(s) 160
TseFI GTSAC 1 cut(s) 32
TseI GCWGC 1 cut(s) 278
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 74, 224
XspI CTAG 1 cut(s) 451
ZraI GACGTC 2 cut(s) 7, 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.