Rroxscaffold_2G00110410

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003465
Physical Location & Seq
Forward (+)
34700778 .. 34701904
1127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00110410.1

Sequence Viewer

Length: 573 bp
ATGAAGATTTTATTTCAATGTGTTGTTGTTTTTCTGGTTTTGATAACTTTAAACGTGAACCCACTGGTGATTTACTTTGATTTCCTTCTCCCTATTAGGTTCACGGAAGATTTTAGTGGATTGTCGTTGGACGAGAACACACTGGCACAAGCTATCAAGCTGCTGATAGACACGAAACATGTTATAATATCAAATTTATCAGGTATCGGAAATGGAACCCTTATGTTGGTTGCTCATGAGACCTTGTGCTTGCGTGTTTCGATTGCTTCCAGCATTGTGGATTTCTTTAAGGAGCTTCCGCAATTTATTGTTAAGGCCGGCCTAATTTTAAGTAATCTGTATGCCCCTTGGTGGTTTGCCAAGTTTCAGTCTTGGTTTCTAGAGAGTGCAATCAGTTGCTGGCATATCATCCATTTTGTAATGAGATACATGAGGGATATCATCCATGATACAACTTTGTCAATTGCAGATAAGTGGGATAGGAGAGCCAACCACACCTATGGTCAAGCGGAACTTGATGAAGTGCGTAATGAGATTGCAAATTATGTGATGAAGAACATGCTGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

190

Amino Acids

21.94

Weight (kDa)

6.28

Isoelectric Point (pI)

25.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
AciI CCGC 2 cut(s) 299, 509
AcsI RAATTY 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 226, 351
AflIII ACRYGT 1 cut(s) 178
AgsI TTSAA 1 cut(s) 17
AluBI AGCT 4 cut(s) 152, 160, 295, 568
AluI AGCT 4 cut(s) 152, 160, 295, 568
Alw26I GTCTC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 399
AoxI GGCC 2 cut(s) 315, 319
ApeKI GCWGC 1 cut(s) 160
ApoI RAATTY 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 79
BbvI GCAGC 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 1 cut(s) 380
BisI GCNGC 1 cut(s) 161
BlsI GCNGC 1 cut(s) 162
BmiI GGNNCC 1 cut(s) 217
BsaI GGTCTC 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 347
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 351
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 69, 147
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 2 cut(s) 408, 441
BseLI CCNNNNNNNGG 2 cut(s) 226, 351
BseNI ACTGG 2 cut(s) 69, 147
BseXI GCAGC 1 cut(s) 147
BshFI GGCC 2 cut(s) 317, 321
BsiSI CCGG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 226, 351
BsmAI GTCTC 1 cut(s) 233
BsnI GGCC 2 cut(s) 317, 321
Bso31I GGTCTC 1 cut(s) 233
BspACI CCGC 2 cut(s) 299, 509
BspANI GGCC 2 cut(s) 317, 321
BspHI TCATGA 1 cut(s) 235
BspLI GGNNCC 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 233
BsrFI RCCGGY 1 cut(s) 317
BsrI ACTGG 2 cut(s) 69, 147
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 347
BstC8I GCNNGC 3 cut(s) 251, 319, 401
BstF5I GGATG 2 cut(s) 408, 441
BstMAI GTCTC 1 cut(s) 233
BstNSI RCATGY 2 cut(s) 182, 562
BstV1I GCAGC 1 cut(s) 147
BstXI CCANNNNNNTGG 2 cut(s) 277, 500
BsuRI GGCC 2 cut(s) 317, 321
BtsCI GGATG 2 cut(s) 408, 441
BtsIMutI CAGTG 2 cut(s) 62, 140
Cac8I GCNNGC 3 cut(s) 251, 319, 401
CaiI CAGNNNCTG 1 cut(s) 399
CciI TCATGA 1 cut(s) 235
Cfr10I RCCGGY 1 cut(s) 317
CviAII CATG 5 cut(s) 179, 236, 430, 446, 559
CviJI RGCY 7 cut(s) 152, 160, 295, 317, 321, 488, 568
CviKI_1 RGCY 7 cut(s) 152, 160, 295, 317, 321, 488, 568
DraI TTTAAA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 347
Eco31I GGTCTC 1 cut(s) 233
Eco32I GATATC 1 cut(s) 439
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 5 cut(s) 182, 239, 433, 449, 562
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FatI CATG 5 cut(s) 178, 235, 429, 445, 558
Fnu4HI GCNGC 1 cut(s) 161
FokI GGATG 2 cut(s) 395, 428
FseI GGCCGGCC 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 1 cut(s) 380
GluI GCNGC 1 cut(s) 161
HaeIII GGCC 2 cut(s) 317, 321
HapII CCGG 1 cut(s) 318
Hin1II CATG 5 cut(s) 182, 239, 433, 449, 562
HpaII CCGG 1 cut(s) 318
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 58, 102
Hpy188I TCNGA 1 cut(s) 209
Hpy188III TCNNGA 2 cut(s) 236, 380
Hpy8I GTNNAC 2 cut(s) 58, 102
HpyAV CCTTC 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 3 cut(s) 389, 467, 539
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 5 cut(s) 182, 239, 433, 449, 562
KroI GCCGGC 1 cut(s) 317
KroNI GCCGGC 1 cut(s) 319
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 7 cut(s) 20, 50, 128, 186, 283, 331, 385
Lsp1109I GCAGC 1 cut(s) 147
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 54
MboII GAAGA 3 cut(s) 16, 119, 565
MfeI CAATTG 1 cut(s) 462
MluCI AATT 5 cut(s) 193, 302, 324, 462, 541
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 1 cut(s) 426
MroNI GCCGGC 1 cut(s) 317
MseI TTAA 4 cut(s) 50, 288, 312, 329
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 1 cut(s) 318
MunI CAATTG 1 cut(s) 462
NaeI GCCGGC 1 cut(s) 319
NgoMIV GCCGGC 1 cut(s) 317
NlaIII CATG 5 cut(s) 182, 239, 433, 449, 562
NlaIV GGNNCC 1 cut(s) 217
NspI RCATGY 2 cut(s) 182, 562
PagI TCATGA 1 cut(s) 235
PciI ACATGT 1 cut(s) 178
PdiI GCCGGC 1 cut(s) 319
PkrI GCNGC 1 cut(s) 162
PscI ACATGT 1 cut(s) 178
PsiI TTATAA 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 217
PstNI CAGNNNCTG 1 cut(s) 399
RigI GGCCGGCC 1 cut(s) 321
RseI CAYNNNNRTG 1 cut(s) 498
SaqAI TTAA 4 cut(s) 50, 288, 312, 329
SatI GCNGC 1 cut(s) 161
SetI ASST 9 cut(s) 57, 101, 154, 162, 205, 245, 297, 500, 570
SmiMI CAYNNNNRTG 1 cut(s) 498
Sse9I AATT 5 cut(s) 193, 302, 324, 462, 541
SsiI CCGC 2 cut(s) 299, 509
SspMI CTAG 1 cut(s) 380
StyI CCWWGG 1 cut(s) 347
TaiI ACGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 260
TasI AATT 5 cut(s) 193, 302, 324, 462, 541
Tru1I TTAA 4 cut(s) 50, 288, 312, 329
Tru9I TTAA 4 cut(s) 50, 288, 312, 329
TscAI CASTG 2 cut(s) 69, 147
TseI GCWGC 1 cut(s) 160
TspDTI ATGAA 3 cut(s) 17, 534, 566
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 2 cut(s) 69, 147
XapI RAATTY 1 cut(s) 193
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 2 cut(s) 182, 562
XspI CTAG 1 cut(s) 380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.