Rh5BG232000

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003465
Physical Location & Seq
Reverse (-)
27361712 .. 27362352
641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG232000.1

Sequence Viewer

Length: 291 bp
ATGAGAAGAGTTCTGATAATTCACAAATTGAGAGCTGCTAAGCTGAACATTGTGGATTTCTTTAAGGAGCTTCCACAGTTTATTGTTAAGGCCGGCCCAATTTTAAGTAATCTGTATGGTGTAGATTGGGAGAGCAAACTTGAGGCAAAGGAGTTGGAGGGCAATTTTCTATACTTGAGCCTTTTAAATCCTGCTACTACTGCTGATTCTCAGGCAAACAAGGAAGCAGCTGATGAATGTTCTGTTTTCGTTGGGAATGTGAGTAAATTTCAATTTGTTTTGACCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

96

Amino Acids

10.76

Weight (kDa)

6.56

Isoelectric Point (pI)

27.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3452 PF11934 10 - 62 3.4e-07 Domain of unknown function (DUF3452)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 266
AgsI TTSAA 1 cut(s) 272
AluBI AGCT 4 cut(s) 35, 43, 70, 230
AluI AGCT 4 cut(s) 35, 43, 70, 230
AoxI GGCC 2 cut(s) 90, 94
ApeKI GCWGC 2 cut(s) 35, 227
ApoI RAATTY 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 95
BbvI GCAGC 2 cut(s) 22, 239
BisI GCNGC 2 cut(s) 36, 228
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 2 cut(s) 37, 229
BmgT120I GGNCC 1 cut(s) 95
Bpu1102I GCTNAGC 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 161, 196
Bse118I RCCGGY 1 cut(s) 92
BseMII CTCAG 1 cut(s) 224
BseXI GCAGC 2 cut(s) 22, 239
BshFI GGCC 2 cut(s) 92, 96
BsiSI CCGG 1 cut(s) 93
BsnI GGCC 2 cut(s) 92, 96
Bsp1720I GCTNAGC 1 cut(s) 39
BspANI GGCC 2 cut(s) 92, 96
BspCNI CTCAG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 92
BssAI RCCGGY 1 cut(s) 92
Bst4CI ACNGT 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 3 cut(s) 39, 210, 288
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstV1I GCAGC 2 cut(s) 22, 239
BsuRI GGCC 2 cut(s) 92, 96
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 92
Cfr13I GGNCC 1 cut(s) 95
CviJI RGCY 7 cut(s) 35, 43, 70, 92, 96, 180, 230
CviKI_1 RGCY 7 cut(s) 35, 43, 70, 92, 96, 180, 230
DdeI CTNAG 3 cut(s) 39, 210, 288
DraI TTTAAA 1 cut(s) 186
FaiI YATR 2 cut(s) 117, 172
Fnu4HI GCNGC 2 cut(s) 36, 228
FseI GGCCGGCC 1 cut(s) 96
Fsp4HI GCNGC 2 cut(s) 36, 228
GluI GCNGC 2 cut(s) 36, 228
HaeIII GGCC 2 cut(s) 92, 96
HapII CCGG 1 cut(s) 93
HinfI GANTC 1 cut(s) 206
HpaII CCGG 1 cut(s) 93
Hpy188I TCNGA 1 cut(s) 15
HpyCH4III ACNGT 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 3 cut(s) 39, 210, 288
KroI GCCGGC 1 cut(s) 92
KroNI GCCGGC 1 cut(s) 94
LmnI GCTCC 1 cut(s) 67
LpnPI CCDG 3 cut(s) 106, 197, 204
Lsp1109I GCAGC 2 cut(s) 22, 239
MboII GAAGA 1 cut(s) 18
MluCI AATT 6 cut(s) 18, 26, 99, 163, 266, 272
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 2 cut(s) 136, 151
MroNI GCCGGC 1 cut(s) 92
MseI TTAA 4 cut(s) 63, 87, 104, 185
MspA1I CMGCKG 1 cut(s) 230
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 1 cut(s) 200
NaeI GCCGGC 1 cut(s) 94
NgoMIV GCCGGC 1 cut(s) 92
PdiI GCCGGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 206
PkrI GCNGC 2 cut(s) 37, 229
PspPI GGNCC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 230
RigI GGCCGGCC 1 cut(s) 96
SaqAI TTAA 4 cut(s) 63, 87, 104, 185
SatI GCNGC 2 cut(s) 36, 228
Sau96I GGNCC 1 cut(s) 95
SetI ASST 5 cut(s) 37, 45, 72, 232, 287
SgeI CNNG 6 cut(s) 105, 152, 187, 203, 224, 232
SmlI CTYRAG 2 cut(s) 140, 175
SmoI CTYRAG 2 cut(s) 140, 175
Sse9I AATT 6 cut(s) 18, 26, 99, 163, 266, 272
TaaI ACNGT 1 cut(s) 78
TasI AATT 6 cut(s) 18, 26, 99, 163, 266, 272
TfiI GAWTC 1 cut(s) 206
Tru1I TTAA 4 cut(s) 63, 87, 104, 185
Tru9I TTAA 4 cut(s) 63, 87, 104, 185
TseI GCWGC 2 cut(s) 35, 227
TspDTI ATGAA 1 cut(s) 249
XapI RAATTY 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.