Rroxscaffold_5G00364850

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003465
Physical Location & Seq
Reverse (-)
46050105 .. 46066480
16376 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00364850.1

Sequence Viewer

Length: 849 bp
ATGGGTTCTGCCTCGGCGGCACAAGAAAATGCTCTTGCTTCCACCACGGATAGGTCTATTTCTATCACCTTGACCGACTTGGATGTGCTTGATTGTCCCATTTTCTATGAATCCTTGACCATCCCCGTCTTTCGGTTACATACATTGGTGGACCCTGTCACTTTAGTAATTGTCCATTTTGTTGGATGTATTCTATTCTCTAAAATCTTTAGTTGTTGGGACCCTGTGGTTGTGTCACATGATTCCGAGGGACATCACACGGATAGCAATTGTCCGGTGATCATCTCACAGATTTTGCCGGTCATCACACGGATAGCCATGATCCGGCGAATCATCACACAAATTTCGCCGGTCATCACACAAATAGAAATCATCACAAAGATTCATCACACCGATTTCAATGATTCATTATACGAGATATTCCTACACAAGCTTTGCATGGCAATCATCACAAAGATTCATCATACCGAGTTCAACGATTCGGTACACGATATTCCTACACAAGCTTTGCATGGCAATCATCACAAAGATTCATCATACCGAGAGCTTCCACAGTTTATTGTTAAGGCCGACCCAATTTTAAGTAATTTGTATGGCGTAGATTGGGAGAGCAAACTTGAGGGAAAAGAGTTGCAGTCAGGCCAATTTTCTGTAGACCCTACTTTTAAGCAAGGTGATATTGATTGGAAATTGAAGTTGAAAGTTGATATTGATTGGACACTAACTCTTAAGACGTTGCTGGAAACTCAAAAGTCCCCCAAGTCCCCGTCGAGGAGGGCAATCTTGGTTGGGGAGTTGTTCGACGGTTTGAAGCGTTTTCTTCCACTAGACTTGCAAGAGCATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

282

Amino Acids

31.92

Weight (kDa)

5.79

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 654
AciI CCGC 1 cut(s) 17
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 1 cut(s) 342
AfaI GTAC 1 cut(s) 486
AfiI CCNNNNNNNGG 4 cut(s) 51, 132, 324, 771
AflII CTTAAG 1 cut(s) 728
AgsI TTSAA 5 cut(s) 400, 475, 694, 700, 811
AluBI AGCT 3 cut(s) 433, 506, 547
AluI AGCT 3 cut(s) 433, 506, 547
AlwI GGATC 1 cut(s) 316
AoxI GGCC 2 cut(s) 567, 640
ApoI RAATTY 1 cut(s) 342
AspS9I GGNCC 2 cut(s) 151, 220
AsuHPI GGTGA 3 cut(s) 58, 289, 686
AvaII GGWCC 2 cut(s) 151, 220
BccI CCATC 1 cut(s) 128
BclI TGATCA 1 cut(s) 279
BfaI CTAG 1 cut(s) 827
BfmI CTRYAG 1 cut(s) 651
BfrI CTTAAG 1 cut(s) 728
BglI GCCNNNNNGGC 1 cut(s) 17
BisI GCNGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 19
Bme18I GGWCC 2 cut(s) 151, 220
BmgT120I GGNCC 2 cut(s) 151, 220
BmiI GGNNCC 3 cut(s) 153, 221, 222
BpuEI CTTGAG 1 cut(s) 638
BsaJI CCNNGG 3 cut(s) 12, 45, 246
BsaWI WCCGGW 1 cut(s) 274
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 132, 324, 771
Bse118I RCCGGY 2 cut(s) 298, 349
BseDI CCNNGG 3 cut(s) 12, 45, 246
BseGI GGATG 3 cut(s) 88, 120, 191
BseLI CCNNNNNNNGG 4 cut(s) 51, 132, 324, 771
BseRI GAGGAG 1 cut(s) 787
BshFI GGCC 2 cut(s) 569, 642
BsiSI CCGG 4 cut(s) 275, 299, 325, 350
BslFI GGGAC 5 cut(s) 81, 233, 264, 739, 748
BslI CCNNNNNNNGG 4 cut(s) 51, 132, 324, 771
BsmFI GGGAC 5 cut(s) 81, 233, 264, 739, 748
BsnI GGCC 2 cut(s) 569, 642
Bsp143I GATC 2 cut(s) 279, 321
BspACI CCGC 1 cut(s) 17
BspANI GGCC 2 cut(s) 569, 642
BspLI GGNNCC 3 cut(s) 153, 221, 222
BspPI GGATC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 728
BsrFI RCCGGY 2 cut(s) 298, 349
BssAI RCCGGY 2 cut(s) 298, 349
BssECI CCNNGG 3 cut(s) 12, 45, 246
BssMI GATC 2 cut(s) 279, 321
Bst4CI ACNGT 2 cut(s) 555, 806
BstAFI CTTAAG 1 cut(s) 728
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 3 cut(s) 88, 120, 191
BstKTI GATC 2 cut(s) 282, 324
BstMBI GATC 2 cut(s) 279, 321
BstMWI GCNNNNNNNGC 1 cut(s) 17
BstSFI CTRYAG 1 cut(s) 651
BstXI CCANNNNNNTGG 1 cut(s) 182
BsuRI GGCC 2 cut(s) 569, 642
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 3 cut(s) 88, 120, 191
Cfr10I RCCGGY 2 cut(s) 298, 349
Cfr13I GGNCC 2 cut(s) 151, 220
Csp6I GTAC 1 cut(s) 485
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 4 cut(s) 239, 319, 439, 512
CviJI RGCY 6 cut(s) 317, 433, 506, 547, 569, 642
CviKI_1 RGCY 6 cut(s) 317, 433, 506, 547, 569, 642
CviQI GTAC 1 cut(s) 485
DpnI GATC 2 cut(s) 281, 323
DpnII GATC 2 cut(s) 279, 321
Eco47I GGWCC 2 cut(s) 151, 220
EcoO109I RGGNCCY 1 cut(s) 220
FaeI CATG 4 cut(s) 242, 322, 442, 515
FaqI GGGAC 5 cut(s) 81, 233, 264, 739, 748
FatI CATG 4 cut(s) 238, 318, 438, 511
FbaI TGATCA 1 cut(s) 279
FblI GTMKAC 1 cut(s) 654
Fnu4HI GCNGC 1 cut(s) 18
FokI GGATG 3 cut(s) 95, 107, 198
Fsp4HI GCNGC 1 cut(s) 18
FspBI CTAG 1 cut(s) 827
GluI GCNGC 1 cut(s) 18
HaeIII GGCC 2 cut(s) 569, 642
HapII CCGG 4 cut(s) 275, 299, 325, 350
Hin1II CATG 4 cut(s) 242, 322, 442, 515
HindIII AAGCTT 2 cut(s) 431, 504
HinfI GANTC 8 cut(s) 110, 242, 330, 382, 404, 457, 479, 530
HpaII CCGG 4 cut(s) 275, 299, 325, 350
HphI GGTGA 3 cut(s) 58, 289, 686
Hpy166II GTNNAC 3 cut(s) 151, 487, 655
Hpy188I TCNGA 1 cut(s) 247
Hpy8I GTNNAC 3 cut(s) 151, 487, 655
Hpy99I CGWCG 2 cut(s) 772, 806
HpyCH4III ACNGT 2 cut(s) 555, 806
HpyCH4IV ACGT 1 cut(s) 734
HpyCH4V TGCA 4 cut(s) 438, 511, 634, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 734
Hsp92II CATG 4 cut(s) 242, 322, 442, 515
KflI GGGWCCC 1 cut(s) 220
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 2 cut(s) 279, 321
LpnPI CCDG 8 cut(s) 168, 237, 288, 312, 338, 363, 624, 725
MaeI CTAG 1 cut(s) 827
MaeII ACGT 1 cut(s) 734
MaeIII GTNAC 3 cut(s) 135, 157, 234
MalI GATC 2 cut(s) 281, 323
MboI GATC 2 cut(s) 279, 321
MboII GAAGA 1 cut(s) 812
MfeI CAATTG 1 cut(s) 268
MluCI AATT 8 cut(s) 168, 268, 342, 576, 586, 644, 689, 844
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 5 cut(s) 22, 241, 613, 765, 768
MseI TTAA 4 cut(s) 564, 581, 666, 729
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 4 cut(s) 275, 299, 325, 350
MunI CAATTG 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 17
NdeII GATC 2 cut(s) 279, 321
NlaIII CATG 4 cut(s) 242, 322, 442, 515
NlaIV GGNNCC 3 cut(s) 153, 221, 222
NmuCI GTSAC 2 cut(s) 157, 234
PfeI GAWTC 8 cut(s) 110, 242, 330, 382, 404, 457, 479, 530
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 220
PspN4I GGNNCC 3 cut(s) 153, 221, 222
PspPI GGNCC 2 cut(s) 151, 220
PspPPI RGGWCCY 1 cut(s) 220
PsyI GACNNNGTC 1 cut(s) 155
RsaI GTAC 1 cut(s) 486
RsaNI GTAC 1 cut(s) 485
SaqAI TTAA 4 cut(s) 564, 581, 666, 729
SatI GCNGC 1 cut(s) 18
Sau3AI GATC 2 cut(s) 279, 321
Sau96I GGNCC 2 cut(s) 151, 220
SetI ASST 7 cut(s) 56, 71, 435, 508, 549, 676, 737
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 2 cut(s) 151, 220
SmlI CTYRAG 2 cut(s) 617, 728
SmoI CTYRAG 2 cut(s) 617, 728
Sse9I AATT 8 cut(s) 168, 268, 342, 576, 586, 644, 689, 844
SsiI CCGC 1 cut(s) 17
SspMI CTAG 1 cut(s) 827
TaaI ACNGT 2 cut(s) 555, 806
TaiI ACGT 1 cut(s) 737
TaqI TCGA 2 cut(s) 770, 801
TaqII GACCGA 1 cut(s) 89
TasI AATT 8 cut(s) 168, 268, 342, 576, 586, 644, 689, 844
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 8 cut(s) 110, 242, 330, 382, 404, 457, 479, 530
Tru1I TTAA 4 cut(s) 564, 581, 666, 729
Tru9I TTAA 4 cut(s) 564, 581, 666, 729
TseFI GTSAC 2 cut(s) 157, 234
Tsp45I GTSAC 2 cut(s) 157, 234
TspDTI ATGAA 5 cut(s) 123, 374, 396, 449, 522
TspGWI ACGGA 3 cut(s) 62, 275, 325
Tth111I GACNNNGTC 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 2 cut(s) 151, 220
XapI RAATTY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 654
XspI CTAG 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.