Rorug07G0279000

NADH kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003465
Physical Location & Seq
Forward (+)
26851406 .. 26852946
1541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0279000.1

Sequence Viewer

Length: 564 bp
ATGGAGAAACTCCAATACGGCTTGGTGCTAACTGTGTCTGTAGTCGCCTCCCTCGGCGTTGTCTCCTTCGTTTCATGCATAGCCGCCGAGATTAAGAGAACAAGGGAGGATGAGGTCAAGTTGGTTGGAAGACTCTGTAAATTGCCGGGAAGTCACGCATTTGGGTTCGGAATTGCAGCATTGATCTGTTTAATTGTGGCTCAGGTGGTCGGAAATTTGGTACTAATATGCAGTCATTTTTGTTCAAGAGAGAAGAAGAGTAGCGGTTCCAAAGCCAAACAACCAATGATTTTGATTGCTCTTCTGTTCATCTCTTGGATGAGCTTTGTAATGGCAGTGACATTGATAAGCACAGCTACTAGCATGAGCAGAAAGCAATCCTATGGAGAAGGATGGCTTGATGGTGAATGCTACCTGGTCAAACAAGGAATCTATATTGGTTCAGGAATACTGATCCTGATTACTGTTGGCTCCACACTAGGCTTAGTGATCATAACTATGAGGAAGAGTCAAGTTGAAGAAGGCTTCAAAGGAGAGCAAAGCAAGCTGAACACCAAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

187

Amino Acids

20.2

Weight (kDa)

9.3

Isoelectric Point (pI)

35.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 60 - 156 1e-20 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 84, 264
AclWI GGATC 1 cut(s) 448
AcsI RAATTY 1 cut(s) 214
AfaI GTAC 1 cut(s) 222
AgsI TTSAA 3 cut(s) 246, 518, 529
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 3 cut(s) 324, 356, 547
AluI AGCT 3 cut(s) 324, 356, 547
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 1 cut(s) 448
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 188
BccI CCATC 2 cut(s) 387, 395
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 416
BclI TGATCA 1 cut(s) 489
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 67
BfaI CTAG 2 cut(s) 360, 479
BfmI CTRYAG 1 cut(s) 39
BisI GCNGC 2 cut(s) 84, 177
BlsI GCNGC 2 cut(s) 85, 178
Bme1390I CCNGG 2 cut(s) 147, 416
BmiI GGNNCC 2 cut(s) 268, 472
BmrFI CCNGG 2 cut(s) 147, 416
BpiI GAAGAC 1 cut(s) 136
Bpu10I CCTNAGC 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 1 cut(s) 52
BseGI GGATG 3 cut(s) 115, 324, 398
BseMII CTCAG 1 cut(s) 215
BseXI GCAGC 1 cut(s) 188
BsiSI CCGG 1 cut(s) 146
BsmAI GTCTC 1 cut(s) 67
BsmI GAATGC 1 cut(s) 413
Bsp143I GATC 3 cut(s) 183, 453, 489
BspACI CCGC 2 cut(s) 84, 264
BspCNI CTCAG 1 cut(s) 214
BspLI GGNNCC 2 cut(s) 268, 472
BspPI GGATC 1 cut(s) 448
BspQI GCTCTTC 1 cut(s) 306
BssECI CCNNGG 1 cut(s) 52
BssMI GATC 3 cut(s) 183, 453, 489
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 2 cut(s) 34, 466
Bst6I CTCTTC 3 cut(s) 251, 306, 500
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 2 cut(s) 201, 484
BstF5I GGATG 3 cut(s) 115, 324, 398
BstKTI GATC 3 cut(s) 186, 456, 492
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 3 cut(s) 183, 453, 489
BstMWI GCNNNNNNNGC 1 cut(s) 544
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 145, 414
BstSFI CTRYAG 1 cut(s) 39
BstV1I GCAGC 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 136
BtsCI GGATG 3 cut(s) 115, 324, 398
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 545
CsiI ACCWGGT 1 cut(s) 414
Csp6I GTAC 1 cut(s) 221
CviAII CATG 2 cut(s) 75, 364
CviQI GTAC 1 cut(s) 221
DdeI CTNAG 2 cut(s) 201, 484
DpnI GATC 3 cut(s) 185, 455, 491
DpnII GATC 3 cut(s) 183, 453, 489
Eam1104I CTCTTC 3 cut(s) 251, 306, 500
EarI CTCTTC 3 cut(s) 251, 306, 500
EcoRII CCWGG 1 cut(s) 414
EcoT22I ATGCAT 1 cut(s) 80
FaeI CATG 2 cut(s) 78, 367
FaiI YATR 8 cut(s) 76, 80, 229, 365, 384, 435, 494, 500
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FatI CATG 2 cut(s) 74, 363
FbaI TGATCA 1 cut(s) 489
Fnu4HI GCNGC 2 cut(s) 84, 177
FokI GGATG 3 cut(s) 122, 331, 405
Fsp4HI GCNGC 2 cut(s) 84, 177
FspBI CTAG 2 cut(s) 360, 479
GluI GCNGC 2 cut(s) 84, 177
HapII CCGG 1 cut(s) 146
Hin1II CATG 2 cut(s) 78, 367
HinfI GANTC 3 cut(s) 132, 429, 508
HpaII CCGG 1 cut(s) 146
HphI GGTGA 1 cut(s) 416
Hpy188I TCNGA 2 cut(s) 170, 212
Hpy188III TCNNGA 3 cut(s) 246, 444, 457
HpyAV CCTTC 3 cut(s) 76, 383, 515
HpyCH4III ACNGT 2 cut(s) 34, 466
HpyCH4V TGCA 3 cut(s) 78, 176, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 544
HpyF3I CTNAG 2 cut(s) 201, 484
Hsp92II CATG 2 cut(s) 78, 367
Ksp22I TGATCA 1 cut(s) 489
Kzo9I GATC 3 cut(s) 183, 453, 489
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 1 cut(s) 476
LpnPI CCDG 6 cut(s) 159, 188, 401, 428, 429, 470
Lsp1109I GCAGC 1 cut(s) 188
MabI ACCWGGT 1 cut(s) 414
MaeI CTAG 2 cut(s) 360, 479
MaeIII GTNAC 2 cut(s) 152, 337
MalI GATC 3 cut(s) 185, 455, 491
MboI GATC 3 cut(s) 183, 453, 489
MboII GAAGA 6 cut(s) 141, 265, 268, 293, 517, 530
MluCI AATT 4 cut(s) 140, 171, 192, 214
MlyI GAGTC 2 cut(s) 126, 517
MmeI TCCRAC 2 cut(s) 106, 190
MnlI CCTC 5 cut(s) 58, 62, 100, 106, 495
Mph1103I ATGCAT 1 cut(s) 80
MseI TTAA 2 cut(s) 93, 191
MslI CAYNNNNRTG 1 cut(s) 497
MspI CCGG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 147, 416
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 1 cut(s) 544
NciI CCSGG 1 cut(s) 147
NdeII GATC 3 cut(s) 183, 453, 489
NlaIII CATG 2 cut(s) 78, 367
NlaIV GGNNCC 2 cut(s) 268, 472
NmeAIII GCCGAG 2 cut(s) 33, 112
NmuCI GTSAC 2 cut(s) 152, 337
NsiI ATGCAT 1 cut(s) 80
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 1 cut(s) 429
PkrI GCNGC 2 cut(s) 85, 178
PleI GAGTC 2 cut(s) 126, 516
PpsI GAGTC 2 cut(s) 126, 516
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 2 cut(s) 268, 472
RsaI GTAC 1 cut(s) 222
RsaNI GTAC 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 2 cut(s) 93, 191
SatI GCNGC 2 cut(s) 84, 177
Sau3AI GATC 3 cut(s) 183, 453, 489
SchI GAGTC 2 cut(s) 126, 517
ScrFI CCNGG 2 cut(s) 147, 416
SetI ASST 6 cut(s) 117, 207, 326, 358, 417, 549
SexAI ACCWGGT 1 cut(s) 414
SfcI CTRYAG 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 497
Sse9I AATT 4 cut(s) 140, 171, 192, 214
SsiI CCGC 2 cut(s) 84, 264
SspMI CTAG 2 cut(s) 360, 479
StyD4I CCNGG 2 cut(s) 145, 414
TaaI ACNGT 2 cut(s) 34, 466
TasI AATT 4 cut(s) 140, 171, 192, 214
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 93, 191
Tru9I TTAA 2 cut(s) 93, 191
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 2 cut(s) 152, 337
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 2 cut(s) 152, 337
TspDTI ATGAA 2 cut(s) 63, 298
TspRI CASTG 1 cut(s) 342
XapI RAATTY 1 cut(s) 214
XspI CTAG 2 cut(s) 360, 479
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.