Rorug07G0278900

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003465
Physical Location & Seq
Forward (+)
26829753 .. 26831716
1964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0278900.1

Sequence Viewer

Length: 606 bp
ATGTCAACGGTGATAGAGAAGTCTGCAGCGGAGGTTGCACCGGGACACGAGTTAGAAGGGATGAGTAGCAACGGCAACGGCAGCAGCTTGAGAACCGCGGAGACGCTGCTGCGACTGCTGCCGATTGCTCCTTGTGTGGCGGCTCTGCTCGTCATGCTTCACGATTCCCAGGACAATGAGTTCGGATCCGTTTCCTACTCTCATATTGGAGCTTTCAGGTTTTTGGTGCATGCCAATGGCATATGTGCTGGTTATTCCCTTCTATCGGCTATCATATCAGCCAAGTCTCGTCCCCCCACCAAGTCTCTAGCCTGGACTTTCTTCTTCATAGATCAATTGCTTACGTACATAATTCTGGGAGCTGGAGCTGTGTCAACGGAGGTTCTGTACTTGGCTTATAAAGGAGATTCAACTATAACGTGGAGTCCGGCTTGTGGGAGTTATGGTGGATTCTGTCACAAAGCCACAACTTCTGTGGTCATTACATTTCTGGTGGTGGCTTGCTTTGTTCTCATTTCACTTATTTCCTCCTACAAACTCTTCAGCAAGTTTGATGCTCCTGCCATCAACTCCATTAAGGGTATTCGAACTGCCACCTTCGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

201

Amino Acids

21.34

Weight (kDa)

7.66

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 28 - 168 9.1e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 399
AccII CGCG 1 cut(s) 98
AciI CCGC 4 cut(s) 29, 96, 98, 140
AclWI GGATC 2 cut(s) 180, 193
AcuI CTGAAG 1 cut(s) 526
AfaI GTAC 2 cut(s) 347, 389
AfiI CCNNNNNNNGG 2 cut(s) 265, 434
AgsI TTSAA 1 cut(s) 411
AjnI CCWGG 2 cut(s) 168, 311
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
AluBI AGCT 4 cut(s) 87, 212, 362, 368
AluI AGCT 4 cut(s) 87, 212, 362, 368
Alw26I GTCTC 3 cut(s) 95, 291, 309
AlwI GGATC 2 cut(s) 180, 193
ApeKI GCWGC 6 cut(s) 26, 81, 84, 106, 109, 118
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 22
AsuII TTCGAA 1 cut(s) 586
BamHI GGATCC 1 cut(s) 185
BauI CACGAG 1 cut(s) 47
BbvI GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BccI CCATC 1 cut(s) 572
BceAI ACGGC 2 cut(s) 88, 94
BciT130I CCWGG 2 cut(s) 170, 313
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 3 cut(s) 95, 291, 309
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
BlsI GCNGC 7 cut(s) 28, 83, 86, 108, 111, 120, 142
Bme1390I CCNGG 3 cut(s) 42, 170, 313
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 3 cut(s) 42, 170, 313
BmsI GCATC 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 586
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 42
BsaAI YACGTR 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 96, 168
BsaXI ACNNNNNCTCC 4 cut(s) 371, 401, 430, 460
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 434
BseBI CCWGG 2 cut(s) 170, 313
BseDI CCNNGG 2 cut(s) 96, 168
BseGI GGATG 1 cut(s) 66
BseLI CCNNNNNNNGG 2 cut(s) 265, 434
BseXI GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
Bsh1236I CGCG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 41, 428
BslFI GGGAC 2 cut(s) 57, 276
BslI CCNNNNNNNGG 2 cut(s) 265, 434
BsmAI GTCTC 3 cut(s) 95, 291, 309
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 2 cut(s) 57, 276
Bsp119I TTCGAA 1 cut(s) 586
Bsp143I GATC 2 cut(s) 185, 331
BspACI CCGC 4 cut(s) 29, 96, 98, 140
BspFNI CGCG 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 28
BspPI GGATC 2 cut(s) 180, 193
BspT104I TTCGAA 1 cut(s) 586
BssECI CCNNGG 2 cut(s) 96, 168
BssMI GATC 2 cut(s) 185, 331
BssSI CACGAG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 47
Bst2UI CCWGG 2 cut(s) 170, 313
Bst4CI ACNGT 1 cut(s) 10
Bst6I CTCTTC 1 cut(s) 545
BstBAI YACGTR 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 2 cut(s) 231, 502
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 66
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 2 cut(s) 188, 334
BstMAI GTCTC 3 cut(s) 95, 291, 309
BstMBI GATC 2 cut(s) 185, 331
BstMWI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
BstNI CCWGG 2 cut(s) 170, 313
BstNSI RCATGY 1 cut(s) 233
BstSCI CCNGG 3 cut(s) 40, 168, 311
BstSFI CTRYAG 1 cut(s) 24
BstSNI TACGTA 1 cut(s) 345
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 231, 502
Cfr42I CCGCGG 1 cut(s) 99
CseI GACGC 1 cut(s) 112
Csp6I GTAC 2 cut(s) 346, 388
CspCI CAANNNNNGTGG 2 cut(s) 456, 491
CviAII CATG 2 cut(s) 154, 230
CviQI GTAC 2 cut(s) 346, 388
DpnI GATC 2 cut(s) 187, 333
DpnII GATC 2 cut(s) 185, 331
Eam1104I CTCTTC 1 cut(s) 545
EarI CTCTTC 1 cut(s) 545
Eco105I TACGTA 1 cut(s) 345
Eco57I CTGAAG 1 cut(s) 526
EcoRII CCWGG 2 cut(s) 168, 311
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 2 cut(s) 157, 233
FaqI GGGAC 2 cut(s) 57, 276
FatI CATG 2 cut(s) 153, 229
FauNDI CATATG 1 cut(s) 242
Fnu4HI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
FokI GGATG 1 cut(s) 73
Fsp4HI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
FspBI CTAG 1 cut(s) 308
GluI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
GsuI CTGGAG 1 cut(s) 384
HapII CCGG 2 cut(s) 41, 428
HgaI GACGC 1 cut(s) 112
Hin1II CATG 2 cut(s) 157, 233
HincII GTYRAC 2 cut(s) 6, 375
HindII GTYRAC 2 cut(s) 6, 375
HinfI GANTC 4 cut(s) 164, 407, 424, 450
HpaII CCGG 2 cut(s) 41, 428
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 6, 375
Hpy188I TCNGA 1 cut(s) 185
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 2 cut(s) 6, 375
HpyAV CCTTC 2 cut(s) 50, 269
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 2 cut(s) 344, 419
HpyCH4V TGCA 3 cut(s) 26, 38, 229
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
HpySE526I ACGT 2 cut(s) 344, 419
Hsp92II CATG 2 cut(s) 157, 233
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 2 cut(s) 185, 331
LmnI GCTCC 5 cut(s) 133, 209, 359, 365, 562
Lsp1109I GCAGC 6 cut(s) 38, 93, 93, 96, 96, 105
LweI GCATC 1 cut(s) 544
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 344, 419
MaeIII GTNAC 1 cut(s) 455
MalI GATC 2 cut(s) 187, 333
MboI GATC 2 cut(s) 185, 331
MboII GAAGA 3 cut(s) 313, 316, 532
MfeI CAATTG 1 cut(s) 335
MflI RGATCY 1 cut(s) 185
MluCI AATT 2 cut(s) 335, 351
MlyI GAGTC 1 cut(s) 433
MnlI CCTC 3 cut(s) 25, 373, 538
MseI TTAA 1 cut(s) 576
MslI CAYNNNNRTG 1 cut(s) 234
MspA1I CMGCKG 2 cut(s) 29, 98
MspI CCGG 2 cut(s) 41, 428
MspR9I CCNGG 3 cut(s) 42, 170, 313
MunI CAATTG 1 cut(s) 335
MvaI CCWGG 2 cut(s) 170, 313
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 5 cut(s) 35, 81, 115, 118, 154
NciI CCSGG 1 cut(s) 42
NdeI CATATG 1 cut(s) 242
NdeII GATC 2 cut(s) 185, 331
NlaIII CATG 2 cut(s) 157, 233
NlaIV GGNNCC 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 1 cut(s) 233
NspV TTCGAA 1 cut(s) 586
PaeI GCATGC 1 cut(s) 233
PfeI GAWTC 3 cut(s) 164, 407, 450
PkrI GCNGC 7 cut(s) 28, 83, 86, 108, 111, 120, 142
PleI GAGTC 1 cut(s) 432
PpsI GAGTC 1 cut(s) 432
Ppu21I YACGTR 1 cut(s) 345
PsiI TTATAA 1 cut(s) 399
Psp6I CCWGG 2 cut(s) 168, 311
PspGI CCWGG 2 cut(s) 168, 311
PspN4I GGNNCC 1 cut(s) 187
PstI CTGCAG 1 cut(s) 28
PsuI RGATCY 1 cut(s) 185
RsaI GTAC 2 cut(s) 347, 389
RsaNI GTAC 2 cut(s) 346, 388
RseI CAYNNNNRTG 1 cut(s) 234
SacII CCGCGG 1 cut(s) 99
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 7 cut(s) 27, 82, 85, 107, 110, 119, 141
Sau3AI GATC 2 cut(s) 185, 331
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 3 cut(s) 42, 170, 313
SfaNI GCATC 1 cut(s) 544
SfcI CTRYAG 1 cut(s) 24
Sfr303I CCGCGG 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 234
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
SnaBI TACGTA 1 cut(s) 345
SphI GCATGC 1 cut(s) 233
Sse9I AATT 2 cut(s) 335, 351
SsiI CCGC 4 cut(s) 29, 96, 98, 140
SspMI CTAG 1 cut(s) 308
StyD4I CCNGG 3 cut(s) 40, 168, 311
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 2 cut(s) 347, 422
TaqI TCGA 1 cut(s) 586
TasI AATT 2 cut(s) 335, 351
TatI WGTACW 1 cut(s) 387
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 3 cut(s) 164, 407, 450
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 6 cut(s) 26, 81, 84, 106, 109, 118
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 1 cut(s) 316
TspGWI ACGGA 2 cut(s) 178, 392
XceI RCATGY 1 cut(s) 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.