Rorug02G0399400

NADH kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003465
Physical Location & Seq
Reverse (-)
51053363 .. 51054709
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0399400.1

Sequence Viewer

Length: 993 bp
ATGATAATGAACTGTCTGGTGCTGTTTTCTCTGACCCTCTTGTCAACTTCGATTCGGGTGATGCTGTTGAACAGCAAGACCTCCTCGAAGAACATGGCCATAGCGAGGCTGCTCAACTTGGTGGACTTCGTGTTCGTAGTCACAGTCTTCTTCAAGCCTATGAAGTTTGGGACAGTTGTTATTGATTCACTTCTCATGGGATTGTACTGCTCCCTTGCTTTTAAGATGAATGGACTTTGCCAGAACATCAGGAACCTAAACTCTGAACTTGAGTCCAAGGCAAAAGACGTTAATTCTGCAGAAGCCAAGACAATTTCCATGAGGAAGCAATCTGAAGAATTGGTTCTTGAGCGTGATCGTTTACTTGAGTCATCGGGCACCAGACTCAAACAACTGGAATCTGAGCTCGAGACCAAGACGAGAGACATTAGTATTGCAAAAGCCAATAATACCGCTCTGAAGAAACAGCTTGATCAGCTTCTTCTCGAGTATGATGTTTTACTGGAGAGAAACCAATTTTTAAAGAATCAGTCGGAGTCATTGAACACAAGAGTCAAAGAGCTAGAGGCTGAAGTCGAGACTAGGACGAAAGATGTTAAAGTTGCAGAAGCCAATCACATTACTCTAAGGAAGCAACTTGAAAAGAGCCTCCTCGAGAATGACCGTCTATCTGAATCGAATCAACTTTTGAACAACCAGTTGCAGTCATCGGGCACAAGACTTCAGCAACTAGAATTTGAACTTGAAACCAAGAGGAATGAAGCTAATGCTGTAGAAGCCAAAATTGTTGCTCTAAGGAAGGAAAAGCAAGATTTAAATATCCAGTTGCAATCATCCAGCACCAGTCTTAGATGTAAAGAATATGAACTCGAGACCAAGACAAAGAATGTTACTACAGCCGAAGCGAAGAATTTAGATCTAAGGAAAGAAATCCAAAGCTTGAAGAACCGGTTACATTCATCAGAAGAACCACTAGAGCAAACCAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00760 ko01100 map00760 map01100
Pfam Domains
Protein Families
NAD_kinase

Protein Analysis

330

Amino Acids

37.7

Weight (kDa)

8.71

Isoelectric Point (pI)

40.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 377
AccBSI CCGCTC 1 cut(s) 455
AciI CCGC 1 cut(s) 453
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 734, 910
AcuI CTGAAG 4 cut(s) 354, 479, 591, 707
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 948
AgsI TTSAA 8 cut(s) 70, 154, 544, 641, 691, 740, 746, 943
AloI GAACNNNNNNTCC 2 cut(s) 116, 148
AluBI AGCT 6 cut(s) 406, 469, 478, 562, 764, 939
AluI AGCT 6 cut(s) 406, 469, 478, 562, 764, 939
Alw21I GWGCWC 1 cut(s) 408
Alw26I GTCTC 4 cut(s) 404, 417, 572, 866
Ama87I CYCGRG 4 cut(s) 407, 485, 653, 869
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 109
ApoI RAATTY 2 cut(s) 734, 910
ArsI GACNNNNNNTTYG 2 cut(s) 116, 148
AsiGI ACCGGT 1 cut(s) 948
Asp700I GAANNNNTTC 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 70
AvaI CYCGRG 4 cut(s) 407, 485, 653, 869
BaeGI GKGCMC 2 cut(s) 380, 716
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 139
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 96
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 4 cut(s) 404, 417, 572, 866
BfaI CTAG 4 cut(s) 563, 582, 731, 974
BfmI CTRYAG 3 cut(s) 297, 771, 894
BglII AGATCT 1 cut(s) 916
BisI GCNGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 111
BmeT110I CYCGRG 4 cut(s) 407, 485, 653, 869
BmiI GGNNCC 2 cut(s) 254, 379
BmsI GCATC 1 cut(s) 51
BpiI GAAGAC 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 524
BpuEI CTTGAG 3 cut(s) 290, 368, 386
BsaI GGTCTC 2 cut(s) 404, 866
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 1 cut(s) 948
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse118I RCCGGY 1 cut(s) 948
Bse1I ACTGG 5 cut(s) 399, 507, 697, 823, 843
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 833
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 5 cut(s) 399, 507, 697, 823, 843
BseRI GAGGAG 2 cut(s) 73, 641
BseSI GKGCMC 2 cut(s) 380, 716
BseXI GCAGC 1 cut(s) 96
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 377
BshTI ACCGGT 1 cut(s) 948
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 4 cut(s) 407, 485, 653, 869
BsiSI CCGG 1 cut(s) 949
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 105
BsmAI GTCTC 4 cut(s) 404, 417, 572, 866
BsmFI GGGAC 1 cut(s) 184
BsnI GGCC 1 cut(s) 98
Bso31I GGTCTC 2 cut(s) 404, 866
BsoBI CYCGRG 4 cut(s) 407, 485, 653, 869
Bsp1286I GDGCHC 3 cut(s) 380, 408, 716
Bsp143I GATC 3 cut(s) 355, 472, 916
BspACI CCGC 1 cut(s) 453
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 1 cut(s) 394
BspLI GGNNCC 2 cut(s) 254, 379
BspMAI CTGCAG 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 377
BspTNI GGTCTC 2 cut(s) 404, 866
BsrBI CCGCTC 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 948
BsrI ACTGG 5 cut(s) 399, 507, 697, 823, 843
BssAI RCCGGY 1 cut(s) 948
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 355, 472, 916
BssT1I CCWWGG 1 cut(s) 276
Bst4CI ACNGT 4 cut(s) 14, 145, 175, 665
BstDEI CTNAG 5 cut(s) 402, 626, 794, 848, 920
BstF5I GGATG 1 cut(s) 833
BstKTI GATC 3 cut(s) 358, 475, 919
BstMAI GTCTC 4 cut(s) 404, 417, 572, 866
BstMBI GATC 3 cut(s) 355, 472, 916
BstMWI GCNNNNNNNGC 2 cut(s) 475, 776
BstSFI CTRYAG 3 cut(s) 297, 771, 894
BstSLI GKGCMC 2 cut(s) 380, 716
BstV1I GCAGC 1 cut(s) 96
BstV2I GAAGAC 1 cut(s) 139
BstX2I RGATCY 1 cut(s) 916
BstYI RGATCY 1 cut(s) 916
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 833
Cfr10I RCCGGY 1 cut(s) 948
Csp6I GTAC 1 cut(s) 205
CspAI ACCGGT 1 cut(s) 948
CviAII CATG 3 cut(s) 94, 196, 319
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 5 cut(s) 402, 626, 794, 848, 920
DpnI GATC 3 cut(s) 357, 474, 918
DpnII GATC 3 cut(s) 355, 472, 916
DraI TTTAAA 2 cut(s) 522, 816
DrdI GACNNNNNNGTC 1 cut(s) 40
DseDI GACNNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 406
Eco130I CCWWGG 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 408
Eco31I GGTCTC 2 cut(s) 404, 866
Eco53kI GAGCTC 1 cut(s) 406
Eco57I CTGAAG 4 cut(s) 354, 479, 591, 707
Eco88I CYCGRG 4 cut(s) 407, 485, 653, 869
EcoICRI GAGCTC 1 cut(s) 406
EcoT14I CCWWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 276
FaeI CATG 3 cut(s) 97, 199, 322
FaiI YATR 7 cut(s) 95, 101, 161, 197, 320, 492, 864
FaqI GGGAC 1 cut(s) 184
FatI CATG 3 cut(s) 93, 195, 318
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 1 cut(s) 110
FokI GGATG 1 cut(s) 820
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 110
FspBI CTAG 4 cut(s) 563, 582, 731, 974
GluI GCNGC 1 cut(s) 110
GsuI CTGGAG 1 cut(s) 524
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 949
Hin1II CATG 3 cut(s) 97, 199, 322
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HindIII AAGCTT 1 cut(s) 937
HpaII CCGG 1 cut(s) 949
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 3 cut(s) 45, 124, 362
Hpy188I TCNGA 8 cut(s) 33, 265, 334, 403, 459, 535, 673, 964
Hpy188III TCNNGA 7 cut(s) 250, 347, 409, 485, 577, 655, 871
Hpy8I GTNNAC 3 cut(s) 45, 124, 362
HpyAV CCTTC 1 cut(s) 793
HpyCH4III ACNGT 4 cut(s) 14, 145, 175, 665
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 5 cut(s) 299, 437, 605, 703, 829
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 776
HpyF3I CTNAG 5 cut(s) 402, 626, 794, 848, 920
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 3 cut(s) 97, 199, 322
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 3 cut(s) 355, 472, 916
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 1 cut(s) 96
LweI GCATC 1 cut(s) 51
MaeI CTAG 4 cut(s) 563, 582, 731, 974
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 139, 889, 951
MalI GATC 3 cut(s) 357, 474, 918
MbiI CCGCTC 1 cut(s) 455
MboI GATC 3 cut(s) 355, 472, 916
MboII GAAGA 9 cut(s) 100, 139, 142, 347, 472, 473, 919, 955, 977
MflI RGATCY 1 cut(s) 916
MhlI GDGCHC 3 cut(s) 380, 408, 716
MlsI TGGCCA 1 cut(s) 98
MluCI AATT 7 cut(s) 292, 312, 338, 515, 734, 783, 910
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 5 cut(s) 281, 377, 378, 545, 561
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 9 cut(s) 47, 91, 94, 99, 315, 559, 659, 662, 747
Mox20I TGGCCA 1 cut(s) 98
MroXI GAANNNNTTC 1 cut(s) 342
MscI TGGCCA 1 cut(s) 98
MseI TTAA 6 cut(s) 222, 291, 521, 597, 815, 991
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 1 cut(s) 949
MwoI GCNNNNNNNGC 2 cut(s) 475, 776
NdeII GATC 3 cut(s) 355, 472, 916
NlaIII CATG 3 cut(s) 97, 199, 322
NlaIV GGNNCC 2 cut(s) 254, 379
NmuCI GTSAC 1 cut(s) 139
PaeR7I CTCGAG 4 cut(s) 407, 485, 653, 869
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 6 cut(s) 52, 185, 398, 526, 674, 679
PinAI ACCGGT 1 cut(s) 948
PkrI GCNGC 1 cut(s) 111
PleI GAGTC 5 cut(s) 280, 376, 378, 544, 560
PpsI GAGTC 5 cut(s) 280, 376, 378, 544, 560
Psp124BI GAGCTC 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 254, 379
PstI CTGCAG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 916
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 6 cut(s) 222, 291, 521, 597, 815, 991
SatI GCNGC 1 cut(s) 110
Sau3AI GATC 3 cut(s) 355, 472, 916
SchI GAGTC 5 cut(s) 281, 377, 378, 545, 561
SduI GDGCHC 3 cut(s) 380, 408, 716
SetI ASST 9 cut(s) 83, 258, 291, 408, 471, 480, 564, 766, 941
SfaNI GCATC 1 cut(s) 51
SfcI CTRYAG 3 cut(s) 297, 771, 894
Sfr274I CTCGAG 4 cut(s) 407, 485, 653, 869
SlaI CTCGAG 4 cut(s) 407, 485, 653, 869
SmiI ATTTAAAT 1 cut(s) 816
SmlI CTYRAG 7 cut(s) 269, 347, 365, 407, 485, 653, 869
SmoI CTYRAG 7 cut(s) 269, 347, 365, 407, 485, 653, 869
Sse9I AATT 7 cut(s) 292, 312, 338, 515, 734, 783, 910
SsiI CCGC 1 cut(s) 453
SspMI CTAG 4 cut(s) 563, 582, 731, 974
SstI GAGCTC 1 cut(s) 408
StyI CCWWGG 1 cut(s) 276
SwaI ATTTAAAT 1 cut(s) 816
TaaI ACNGT 4 cut(s) 14, 145, 175, 665
TaiI ACGT 1 cut(s) 291
TaqI TCGA 8 cut(s) 50, 86, 408, 486, 576, 654, 677, 870
TasI AATT 7 cut(s) 292, 312, 338, 515, 734, 783, 910
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 6 cut(s) 52, 185, 398, 526, 674, 679
Tru1I TTAA 6 cut(s) 222, 291, 521, 597, 815, 991
Tru9I TTAA 6 cut(s) 222, 291, 521, 597, 815, 991
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 6 cut(s) 23, 176, 242, 774, 879, 948
XapI RAATTY 2 cut(s) 734, 910
XhoI CTCGAG 4 cut(s) 407, 485, 653, 869
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 4 cut(s) 563, 582, 731, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.