Rroxscaffold_3G00224390

Retinoblastoma-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003465
Physical Location & Seq
Reverse (-)
7491541 .. 7493700
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224390.1

Sequence Viewer

Length: 666 bp
ATGGAGCGACCGGCGAGCTCGTTCGAGCTCAACCAAGGAAACCCGAAGTCGTGCCGACGTCGGAGCTGCGTTTTCCGGCCGGGGTCAAGCTTCGTACAACCTGTTCGCCCTTGCAGCGCCGCCGTAGGCTTGAATCGTCGACGGGAGACCACCATGGAGACGATCGGAAGGAGGAGGCGACCCCGCCGGTTCACGGAAGATTTCAGTGGATTGTTGTTGGACGAGAACACACCGGCACAAGCTATCAAGCTGCTGATAGACACGAAACATGTTATAATATCAAATTTATCAGGTATCGGAAATGGAACCCCCGAAAGAAGCAGAACGGTTCTGGTTTTTGTTTGTTTTGTTCTCGGTGAAAACATTGAGCAAGAAGAGTTCGATAATTCACAAAAGGGGTCTAATAATGGTGGATTCTCTCTCATCCAAATTTTGAGAGCTGCTAAGCTAAACTTATGTTGGTTGCTCATGAGACCTTTGTGCTTGCGTGTTTCGGTTGCTTCCAGCATTGTGGATTTCTTTAAGGAGCTTCCGCAATTTATTGTTAAGGCCGGCCTAATTTTAATGGGGGATAGGAGAGCCAACCACACCTATGGTCAAGCGGAACTTGATGAAGTGCGTAATGAGATTGCAAATTATGTGATGAAGAACATGCTGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000082 GO:0000278 GO:0000280 GO:0000902 GO:0000904 GO:0001558 GO:0001708 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006275 GO:0006349 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008356 GO:0009553 GO:0009555 GO:0009566 GO:0009567 GO:0009653 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010026 GO:0010052 GO:0010090 GO:0010103 GO:0010374 GO:0010377 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0019219 GO:0019222 GO:0019953 GO:0022402 GO:0022414 GO:0022619 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0033043 GO:0034641 GO:0040008 GO:0040029 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0044770 GO:0044772 GO:0044843 GO:0045132 GO:0045143 GO:0045165 GO:0046483 GO:0048229 GO:0048285 GO:0048366 GO:0048367 GO:0048468 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051128 GO:0051171 GO:0051239 GO:0051276 GO:0051301 GO:0051321 GO:0051704 GO:0051726 GO:0051783 GO:0055046 GO:0060255 GO:0061982 GO:0065007 GO:0070192 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097159 GO:0098813 GO:0099402 GO:0140013 GO:1901360 GO:1901363 GO:1903046 GO:1903047 GO:1903866 GO:2000036 GO:2000112 GO:2000653
KEGG Pathways
Metabolic & Signaling
ko04110 ko04218 ko04350 ko05165 ko05203 map04110 map04218 map04350 map05165 map05203
Pfam Domains
Protein Families
DUF3452 RB_A RB_B Rb_C

Protein Analysis

221

Amino Acids

24.95

Weight (kDa)

9.73

Isoelectric Point (pI)

59.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
AatII GACGTC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 139
AciI CCGC 4 cut(s) 120, 184, 533, 602
AcoI YGGCCR 1 cut(s) 77
AcsI RAATTY 2 cut(s) 283, 429
AcyI GRCGYC 1 cut(s) 58
AfaI GTAC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 1 cut(s) 133
Alw21I GWGCWC 2 cut(s) 20, 30
Alw26I GTCTC 3 cut(s) 140, 152, 466
AoxI GGCC 3 cut(s) 77, 549, 553
ApeKI GCWGC 4 cut(s) 66, 114, 250, 440
ApoI RAATTY 2 cut(s) 283, 429
AspLEI GCGC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 1 cut(s) 368
BanII GRGCYC 2 cut(s) 20, 30
Bbv12I GWGCWC 2 cut(s) 20, 30
BbvI GCAGC 4 cut(s) 53, 126, 237, 427
BceAI ACGGC 1 cut(s) 107
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 3 cut(s) 140, 152, 466
BfoI RGCGCY 1 cut(s) 120
BisI GCNGC 5 cut(s) 67, 115, 120, 251, 441
BlpI GCTNAGC 1 cut(s) 444
BlsI GCNGC 5 cut(s) 68, 116, 121, 252, 442
Bme1390I CCNGG 1 cut(s) 81
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 1 cut(s) 81
Bpu1102I GCTNAGC 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 81
BsaHI GRCGYC 1 cut(s) 58
BsaI GGTCTC 2 cut(s) 140, 466
BsaJI CCNNGG 3 cut(s) 34, 80, 153
Bsc4I CCNNNNNNNGG 1 cut(s) 193
Bse118I RCCGGY 4 cut(s) 10, 186, 232, 551
BseDI CCNNGG 3 cut(s) 34, 80, 153
BseGI GGATG 1 cut(s) 423
BseLI CCNNNNNNNGG 1 cut(s) 193
BseRI GAGGAG 1 cut(s) 187
BseX3I CGGCCG 1 cut(s) 77
BseXI GCAGC 4 cut(s) 53, 126, 237, 427
Bsh1285I CGRYCG 3 cut(s) 11, 80, 165
BshFI GGCC 3 cut(s) 79, 551, 555
BsiEI CGRYCG 3 cut(s) 11, 80, 165
BsiHKAI GWGCWC 2 cut(s) 20, 30
BsiSI CCGG 6 cut(s) 11, 76, 80, 187, 233, 552
BslI CCNNNNNNNGG 1 cut(s) 193
BsmAI GTCTC 3 cut(s) 140, 152, 466
BsmBI CGTCTC 1 cut(s) 152
BsnI GGCC 3 cut(s) 79, 551, 555
Bso31I GGTCTC 2 cut(s) 140, 466
Bsp1286I GDGCHC 2 cut(s) 20, 30
Bsp143I GATC 1 cut(s) 162
Bsp1720I GCTNAGC 1 cut(s) 444
Bsp19I CCATGG 1 cut(s) 153
BspACI CCGC 4 cut(s) 120, 184, 533, 602
BspANI GGCC 3 cut(s) 79, 551, 555
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 1 cut(s) 307
BspTNI GGTCTC 2 cut(s) 140, 466
BsrFI RCCGGY 4 cut(s) 10, 186, 232, 551
BssAI RCCGGY 4 cut(s) 10, 186, 232, 551
BssECI CCNNGG 3 cut(s) 34, 80, 153
BssMI GATC 1 cut(s) 162
BssNI GRCGYC 1 cut(s) 58
BssT1I CCWWGG 2 cut(s) 34, 153
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 1 cut(s) 369
BstACI GRCGYC 1 cut(s) 58
BstC8I GCNNGC 3 cut(s) 16, 485, 553
BstDEI CTNAG 1 cut(s) 444
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 1 cut(s) 423
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 1 cut(s) 165
BstMAI GTCTC 3 cut(s) 140, 152, 466
BstMBI GATC 1 cut(s) 162
BstMCI CGRYCG 3 cut(s) 11, 80, 165
BstMWI GCNNNNNNNGC 1 cut(s) 114
BstNSI RCATGY 2 cut(s) 272, 655
BstSCI CCNGG 1 cut(s) 79
BstV1I GCAGC 4 cut(s) 53, 126, 237, 427
BstXI CCANNNNNNTGG 2 cut(s) 511, 593
BstZI CGGCCG 1 cut(s) 77
BsuRI GGCC 3 cut(s) 79, 551, 555
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 211
Cac8I GCNNGC 3 cut(s) 16, 485, 553
CciI TCATGA 1 cut(s) 468
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 4 cut(s) 10, 186, 232, 551
Csp6I GTAC 1 cut(s) 95
CviAII CATG 4 cut(s) 154, 269, 469, 652
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 1 cut(s) 444
DpnI GATC 1 cut(s) 164
DpnII GATC 1 cut(s) 162
EaeI YGGCCR 1 cut(s) 77
EagI CGGCCG 1 cut(s) 77
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Ecl136II GAGCTC 2 cut(s) 18, 28
EclXI CGGCCG 1 cut(s) 77
Eco130I CCWWGG 2 cut(s) 34, 153
Eco24I GRGCYC 2 cut(s) 20, 30
Eco31I GGTCTC 2 cut(s) 140, 466
Eco52I CGGCCG 1 cut(s) 77
Eco53kI GAGCTC 2 cut(s) 18, 28
EcoICRI GAGCTC 2 cut(s) 18, 28
EcoT14I CCWWGG 2 cut(s) 34, 153
EcoT38I GRGCYC 2 cut(s) 20, 30
ErhI CCWWGG 2 cut(s) 34, 153
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 4 cut(s) 157, 272, 472, 655
FaiI YATR 8 cut(s) 155, 270, 275, 457, 470, 594, 639, 653
FalI AAGNNNNNCTT 4 cut(s) 437, 469, 591, 623
FatI CATG 4 cut(s) 153, 268, 468, 651
FauI CCCGC 1 cut(s) 191
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 5 cut(s) 67, 115, 120, 251, 441
FokI GGATG 1 cut(s) 410
FriOI GRGCYC 2 cut(s) 20, 30
FseI GGCCGGCC 1 cut(s) 555
Fsp4HI GCNGC 5 cut(s) 67, 115, 120, 251, 441
GlaI GCGC 1 cut(s) 118
GluI GCNGC 5 cut(s) 67, 115, 120, 251, 441
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 3 cut(s) 79, 551, 555
HapII CCGG 6 cut(s) 11, 76, 80, 187, 233, 552
HhaI GCGC 1 cut(s) 119
Hin1I GRCGYC 1 cut(s) 58
Hin1II CATG 4 cut(s) 157, 272, 472, 655
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 140
HindII GTYRAC 1 cut(s) 140
HindIII AAGCTT 1 cut(s) 88
HinfI GANTC 2 cut(s) 133, 414
HpaII CCGG 6 cut(s) 11, 76, 80, 187, 233, 552
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 2 cut(s) 140, 192
Hpy188I TCNGA 3 cut(s) 63, 167, 299
Hpy188III TCNNGA 1 cut(s) 469
Hpy8I GTNNAC 2 cut(s) 140, 192
Hpy99I CGWCG 4 cut(s) 60, 63, 141, 144
HpyAV CCTTC 1 cut(s) 162
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 2 cut(s) 114, 632
HpyF10VI GCNNNNNNNGC 1 cut(s) 114
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 1 cut(s) 58
Hsp92I GRCGYC 1 cut(s) 58
Hsp92II CATG 4 cut(s) 157, 272, 472, 655
HspAI GCGC 1 cut(s) 117
KroI GCCGGC 1 cut(s) 551
KroNI GCCGGC 1 cut(s) 553
Kzo9I GATC 1 cut(s) 162
LmnI GCTCC 3 cut(s) 4, 63, 526
Lsp1109I GCAGC 4 cut(s) 53, 126, 237, 427
MaeII ACGT 1 cut(s) 58
MalI GATC 1 cut(s) 164
MboI GATC 1 cut(s) 162
MboII GAAGA 3 cut(s) 209, 386, 658
MhlI GDGCHC 2 cut(s) 20, 30
MluCI AATT 6 cut(s) 283, 385, 429, 536, 558, 634
MmeI TCCRAC 2 cut(s) 41, 198
MnlI CCTC 2 cut(s) 165, 168
MroNI GCCGGC 1 cut(s) 551
MseI TTAA 3 cut(s) 522, 546, 563
MslI CAYNNNNRTG 1 cut(s) 591
MspI CCGG 6 cut(s) 11, 76, 80, 187, 233, 552
MspR9I CCNGG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 114
NaeI GCCGGC 1 cut(s) 553
NciI CCSGG 1 cut(s) 81
NcoI CCATGG 1 cut(s) 153
NdeII GATC 1 cut(s) 162
NgoMIV GCCGGC 1 cut(s) 551
NlaIII CATG 4 cut(s) 157, 272, 472, 655
NlaIV GGNNCC 1 cut(s) 307
NspI RCATGY 2 cut(s) 272, 655
PagI TCATGA 1 cut(s) 468
PciI ACATGT 1 cut(s) 268
PdiI GCCGGC 1 cut(s) 553
PfeI GAWTC 2 cut(s) 133, 414
PkrI GCNGC 5 cut(s) 68, 116, 121, 252, 442
Ple19I CGATCG 1 cut(s) 165
PscI ACATGT 1 cut(s) 268
PsiI TTATAA 1 cut(s) 275
Psp124BI GAGCTC 2 cut(s) 20, 30
PspN4I GGNNCC 1 cut(s) 307
PsrI GAACNNNNNNTAC 2 cut(s) 87, 119
PvuI CGATCG 1 cut(s) 165
RigI GGCCGGCC 1 cut(s) 555
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 591
SacI GAGCTC 2 cut(s) 20, 30
SalI GTCGAC 1 cut(s) 138
SaqAI TTAA 3 cut(s) 522, 546, 563
SatI GCNGC 5 cut(s) 67, 115, 120, 251, 441
Sau3AI GATC 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 2 cut(s) 20, 30
SgrDI CGTCGACG 1 cut(s) 138
SmiMI CAYNNNNRTG 1 cut(s) 591
Sse9I AATT 6 cut(s) 283, 385, 429, 536, 558, 634
SsiI CCGC 4 cut(s) 120, 184, 533, 602
SstI GAGCTC 2 cut(s) 20, 30
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 2 cut(s) 34, 153
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 1 cut(s) 61
TaqI TCGA 3 cut(s) 24, 139, 381
TasI AATT 6 cut(s) 283, 385, 429, 536, 558, 634
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 2 cut(s) 133, 414
Tru1I TTAA 3 cut(s) 522, 546, 563
Tru9I TTAA 3 cut(s) 522, 546, 563
TscAI CASTG 1 cut(s) 211
TseI GCWGC 4 cut(s) 66, 114, 250, 440
TspDTI ATGAA 2 cut(s) 627, 659
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 1 cut(s) 211
XapI RAATTY 2 cut(s) 283, 429
XceI RCATGY 2 cut(s) 272, 655
XmiI GTMKAC 1 cut(s) 139
ZraI GACGTC 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.