Rorug02G0465500

Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000059
Physical Location & Seq
Reverse (-)
59664051 .. 59667661
3611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0465500.1

Sequence Viewer

Length: 2151 bp
ATGGGAAAAAAGAAGACAGATGAAGCTGGTGCTGCCATGAAGGGTAAGTCAACCGGAAAAGATGCTTCTAAAGATGGAAAGAAAGTGAAAGCACCAACAGTCTCTGCCATGTTGGCCAACATGGATCAGAAACCTGAGAAGCCCAAGAAGGCATCTTCCTCCAGTGCAAAGGCAAAAGTCGCTTCAAAACTTTCATCCTACACTGATGGTATTGATCTCCCTCCCTCTGATGATGAGGTGGAAGAGGAACAACAATCCAATCAGCAAAGGAGGTCAGAGGCTAAGACGCTTGATGTATCCATAACAGAGAAAGAGTTAAAAAAACGTGAAAAGAAGGACCTTCTTGCTGCTCATGCTGCTGAGCTGTCAAAGAAGGAGGCCCTGAGAGATGATCATGATGCTTTCACTGTTGTTATTGGAAGCAGGGCTTCAGTACTTGAGGGTGAAGATGCAGATGCTAATATCAAGGATATATCAATAGATAGTTTTTCTGTGGCGGCTCGAGGAAAGGAACTACTAAAGAATACATCAGTGAAGATTTCTCATGGGAAGAGATATGGTCTGATTGGTCCAAATGGGATGGGGAAGTCTACCCTTCTGAAGCTTCTTGCTTGGAGGAAGATTCCAGTACCAAAAAACATCGATGTTCTTTTAGTTGAACAGGAGGTAGTTGGTGATGATAGAAGTGCTCTGGAAGCTGTTGTTTCAGCTAATGAAGAACTTGTCAAGCTCCGGGAAGAGGTTGCAGCATTGCAGAATTCAGATTCTGCTCCCCGTGGTGAGGAAGAGGATAGTCATGATGATGATGCAGGAGAGAAGCTTGCTGAGTTGTACGATCAATTGCAGCTGATGGGATCGGATGCTGCTGAAGCCCAGGCTTCAAAGATTCTTGCCGGTTTGGGGTTTACAAAGGATATGCAAGGCCGCCCCACTAAGTCATTCAGTGGTGGTTGGAGAATGAGAATTTCATTAGCTAGGGCACTTTTTGTGCAACCAACTCTTTTGTTGCTTGATGAACCAACAAATCATCTCGACTTGAGGGCTGTTCTTTGGTTGGAGGAGTATTTGTGCCGCTGGAAAAAAACTTTGGTGGTTGTTTCACACGATCGTGATTTCCTTAACACAGTGTGCACAGAGATTATTCATCTACATGATCTGAAGCTCCAGTTCTACCGTGGAAATTTTGATGATTTTGAAACTGGCTATGAGCAGCGCCGCAAAGAGGTAAACAAGAAGTTTGAAATTTATGATAAGCAGGTGAAAGCTGCCAGAAGGAGCGGAAGTCGGGTTCAACAGGAGAAGGTCAAGGATCGAGCAAAATTCTTAGTTAATAAGGAAGCATCAAAGAGCAAGGGCAAGGGAAAGGTTGAGGAGGATGACACCCAAGTTGAGGCTCCTAAGAAGTGGAGGGATTACAGTGTGCAGTTCCACTTTCCTGAACCTACTGAGCTTACACCTCCTCTCTTGCAACTTATTGATGTCAGCTTCAGTTATCCGAACCGGGAAGATTTCAGGCTGTCTGATGTTGATGTCGGTATTGATATGGGTACTCGTGTTGCAATTGTTGGACCTAACGGAGCCGGAAAGTCTACTCTGCTCAACCTTCTTGCAGGTGATCTGGTTCCTACTGAGGGTGAAGTAAGGAGAAGTCAGAAGTTGAGGATTGGTAGGTATTCACAGCACTTTGTGGACCTCCTGACAATGGATGAAACCCCAGTTTCATACCTTCTTCGTCTTCATCCTGACCAAGAGGGATTTAGCAAGCAGGAGGCTGTGCGTGCAAAACTTGGAAAGTTTGGACTCCCTAGCCACAACCATCTGACTCCTATTGCAAAATTATCCGGAGGACAGAAAGCTAGAGTTGTCTTCACATCAATATCCATGTCAAAACCCCACATTCTGCTTTTGGATGAACCCACGAATCATTTAGATATGCAGAGCATTGATGCACTGGCTGATGCACTTGATGAATTCACCGGTGGGGTTGTACTGGTCAGTCACGACTCTAGACTTATTTCTCGTGTGTGTGATGATGAAGAGAGGAGTCAAATTTGGATTGTTGAAGATGGTACTGTGAAGAAATTTGATAGAAGTTTTGATGAGTACAAGGATGAGCTACAAAAAGCAATTAGAGATGAGGTGGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FA_desaturase

Protein Analysis

716

Amino Acids

79.83

Weight (kDa)

5.94

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 173 - 342 6.8e-25 ABC transporter
ABC_tran PF00005 506 - 641 8.3e-24 ABC transporter
AAA_21 PF13304 576 - 672 9.1e-06 AAA domain, putative AbiEii toxin, Type IV TA system
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06080 AT1G06080 AT2G31360 AT2G31360
fragaria_vesca FvH4_1g04480 FvH4_1g04480 FvH4_1g04480 FvH4_1g04480 FvH4_3g00350 FvH4_3g28040 FvH4_3g28040 FvH4_3g28040 FvH4_3g28050 FvH4_5g34260 FvH4_6g42131 FvH4_6g42140 FvH4_6g42171 FvH4_6g42171 FvH4_6g42171 FvH4_6g42171 FvH4_6g42171 FvH4_6g42311 FvH4_6g42312 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640
malus_domestica MD00G1098700.v1.1 MD00G1139800.v1.1 MD00G1174400.v1.1 MD02G1133600.v1.1 MD02G1271200.v1.1 MD09G1114000.v1.1 MD09G1114100.v1.1 MD09G1114200.v1.1 MD11G1194300.v1.1 MD15G1438800.v1.1 MD17G1105800.v1.1 MD17G1105900.v1.1
prunus_persica Prupe.3G210300_v2.0.a1 Prupe.3G210300_v2.0.a1 Prupe.3G210400_v2.0.a1 Prupe.3G210500_v2.0.a1 Prupe.3G210600_v2.0.a1 Prupe.3G210600_v2.0.a1 Prupe.4G228700_v2.0.a1 Prupe.4G228800_v2.0.a1 Prupe.4G228900_v2.0.a1 Prupe.4G229000_v2.0.a1 Prupe.4G229300_v2.0.a1 Prupe.4G229500_v2.0.a1 Prupe.4G229600_v2.0.a1 Prupe.4G259100_v2.0.a1 Prupe.4G259400_v2.0.a1 Prupe.4G259500_v2.0.a1 Prupe.4G259600_v2.0.a1 Prupe.4G259600_v2.0.a1 Prupe.4G259600_v2.0.a1 Prupe.4G259800_v2.0.a1 Prupe.4G259900_v2.0.a1
pyrus_communis pycom03g12440 pycom09g03830 pycom09g03840 pycom09g03850 pycom11g16300 pycom15g38680 pycom17g09850
rosa_chinensis RchiOBHm_Chr1g0337221 RchiOBHm_Chr1g0343481 RchiOBHm_Chr1g0343491 RchiOBHm_Chr1g0343531 RchiOBHm_Chr1g0343551 RchiOBHm_Chr1g0343711 RchiOBHm_Chr1g0343721 RchiOBHm_Chr1g0343731 RchiOBHm_Chr1g0360301 RchiOBHm_Chr2g0086981 RchiOBHm_Chr2g0142871 RchiOBHm_Chr2g0156531 RchiOBHm_Chr2g0157491 RchiOBHm_Chr2g0157791 RchiOBHm_Chr2g0158051 RchiOBHm_Chr2g0158061 RchiOBHm_Chr2g0158141 RchiOBHm_Chr2g0158151 RchiOBHm_Chr2g0158221 RchiOBHm_Chr2g0158251 RchiOBHm_Chr2g0159451 RchiOBHm_Chr4g0403611 RchiOBHm_Chr4g0412621 RchiOBHm_Chr4g0424961 RchiOBHm_Chr4g0425001 RchiOBHm_Chr4g0425091 RchiOBHm_Chr5g0012311 RchiOBHm_Chr5g0022121 RchiOBHm_Chr5g0050081 RchiOBHm_Chr5g0050721 RchiOBHm_Chr5g0050731 RchiOBHm_Chr5g0050761 RchiOBHm_Chr6g0252581 RchiOBHm_Chr7g0199141 RchiOBHm_Chr7g0216811 RchiOBHm_Chr7g0234331 RchiOBHm_Chr7g0234341 RchiOBHm_Chr7g0234371 RchiOBHm_Chr7g0234431 RchiOBHm_Chr7g0234681 RchiOBHm_Chr7g0234741
rosa_laevigata RLG00000001215 RLG00000001222 RLG00000001223 RLG00000001229 RLG00000003811 RLG00000007410 RLG00000015084 RLG00000017931 RLG00000020899 RLG00000020919 RLG00000020994 RLG00000021020 RLG00000021055 RLG00000021056 RLG00000021057 RLG00000021063 RLG00000021065 RLG00000021066 RLG00000021069 RLG00000021071 RLG00000021156 RLG00000024999 RLG00000025005 RLG00000028964 RLG00000029292 RLG00000032646 RLG00000034725 RLG00000034726 RLG00000034730
rosa_multiflora Rmu_co8371849.1_g000001 Rmu_co8422897.1_g000001 Rmu_sc0000195.1_g000039 Rmu_sc0000333.1_g000003 Rmu_sc0000419.1_g000030 Rmu_sc0000419.1_g000034 Rmu_sc0002016.1_g000040 Rmu_sc0002117.1_g000018 Rmu_sc0002117.1_g000023 Rmu_sc0002160.1_g000002 Rmu_sc0002204.1_g000008 Rmu_sc0002775.1_g000001 Rmu_sc0002775.1_g000013 Rmu_sc0002801.1_g000001 Rmu_sc0002801.1_g000013 Rmu_sc0004434.1_g000003 Rmu_sc0004864.1_g000019 Rmu_sc0006966.1_g000006 Rmu_sc0007763.1_g000002 Rmu_sc0007769.1_g000006 Rmu_sc0007769.1_g000013 Rmu_sc0008904.1_g000001 Rmu_sc0013250.1_g000001 Rmu_sc0013327.1_g000004 Rmu_sc0037251.1_g000001 Rmu_ssc0000217.1_g000013 Rmu_ssc0000420.1_g000014 Rmu_ssc0000420.1_g000016
rosa_roxburghii Rroxscaffold_1G00030000 Rroxscaffold_1G00030030 Rroxscaffold_1G00030060 Rroxscaffold_2G00090560 Rroxscaffold_2G00090580 Rroxscaffold_2G00091590 Rroxscaffold_2G00091630 Rroxscaffold_2G00091670 Rroxscaffold_2G00091700 Rroxscaffold_2G00091950 Rroxscaffold_2G00092160 Rroxscaffold_2G00132010 Rroxscaffold_3G00226740 Rroxscaffold_3G00226760 Rroxscaffold_5G00356990 Rroxscaffold_5G00367150 Rroxscaffold_5G00368290 Rroxscaffold_7G00212540
rosa_rugosa Rorug01G0132700.1 Rorug01G0132800.1 Rorug01G0132900.1 Rorug01G0133000.1 Rorug01G0133100.1 Rorug01G0133200.1 Rorug01G0167200 Rorug01G0471200 Rorug02G0461800 Rorug02G0463400 Rorug02G0463500 Rorug02G0463600 Rorug02G0463700 Rorug02G0463700 Rorug02G0463700 Rorug02G0465500 Rorug02G0465800 Rorug02G0465900.1 Rorug02G0466000 Rorug02G0466100 Rorug02G0466200 Rorug02G0466300 Rorug02G0466600 Rorug02G0475900 Rorug04G0200800 Rorug04G0201000 Rorug04G0201100 Rorug04G0384700 Rorug05G0000500 Rorug05G0257600 Rorug05G0257600 Rorug05G0263300 Rorug05G0263700 Rorug05G0537100 Rorug05G0579200 Rorug05G0579200 Rorug07G0155500 Rorug07G0163100 Rorug07G0283400 Rorug07G0284800 Rorug07G0285400
rosa_samantha Rh1AG184700 Rh1BG152200 Rh1DG184100 Rh2AG024000 Rh2AG525800 Rh2AG529100 Rh2AG531600 Rh2AG531800 Rh2AG532600 Rh2AG532700 Rh2AG542500 Rh2BG024600 Rh2BG540500 Rh2BG543000 Rh2BG546000 Rh2BG546100 Rh2BG546500 Rh2BG555700 Rh2CG024700 Rh2CG510500 Rh2CG513400 Rh2CG515800 Rh2CG515900 Rh2CG516300 Rh2CG516400 Rh2CG525100 Rh2CG525900 Rh2DG549000 Rh2DG551900 Rh2DG554300 Rh2DG554500 Rh2DG555100 Rh2DG564800 Rh2DG565100 Rh4AG174400 Rh4AG258000 Rh4BG263600 Rh4CG274800 Rh4DG170100 Rh4DG259200 Rh4DG259500 Rh5AG094500 Rh5AG159500 Rh5AG330300 Rh5AG335300 Rh5AG335400 Rh5AG335800 Rh5BG090700 Rh5BG159000 Rh5BG341000 Rh5BG344600 Rh5BG344800 Rh5BG345200 Rh5CG173200 Rh5CG372600 Rh5CG372700 Rh5CG373100 Rh5DG090200 Rh5DG159000 Rh5DG353800 Rh5DG357700 Rh5DG357900 Rh5DG358300 Rh6AG053400 Rh7AG176500 Rh7AG301900 Rh7AG439800 Rh7BG294700 Rh7BG412800 Rh7CG185600 Rh7CG320600 Rh7CG460400 Rh7CG460500 Rh7CG460900 Rh7CG461300 Rh7DG179500 Rh7DG303400 Rh7DG429500 Rh7DG429600
rosa_wichuraiana Rw0G019480 Rw1G012510 Rw1G015180 Rw2G001890 Rw2G043550 Rw2G043740 Rw2G043960 Rw2G043970 Rw2G044020 Rw2G044030 Rw2G044080 Rw2G044100 Rw2G044850 Rw2G044910 Rw4G014610 Rw4G022270 Rw4G022280 Rw4G022310 Rw5G008250 Rw5G014230 Rw5G031190 Rw5G031640 Rw5G031650 Rw5G031680 Rw6G004800 Rw7G015520 Rw7G025750 Rw7G036370 Rw7G036390 Rw7G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1246, 1601
Acc36I ACCTGC 2 cut(s) 1246, 1601
AccBSI CCGCTC 1 cut(s) 1278
AccI GTMKAC 2 cut(s) 590, 1589
AccIII TCCGGA 1 cut(s) 1841
AciI CCGC 5 cut(s) 497, 925, 1072, 1216, 1278
AclWI GGATC 3 cut(s) 132, 862, 1317
AcoI YGGCCR 1 cut(s) 114
AcsI RAATTY 8 cut(s) 757, 963, 1180, 1242, 1319, 1970, 2049, 2081
AcuI CTGAAG 5 cut(s) 414, 620, 888, 1178, 1471
AdeI CACNNNGTG 2 cut(s) 1128, 1687
AfaI GTAC 7 cut(s) 435, 630, 833, 1549, 1989, 2071, 2105
AfiI CCNNNNNNNGG 7 cut(s) 739, 781, 900, 1222, 1390, 1631, 1702
AgeI ACCGGT 1 cut(s) 1976
AgsI TTSAA 7 cut(s) 186, 659, 882, 1196, 1241, 1292, 2063
AjnI CCWGG 1 cut(s) 873
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AleI CACNNNNGTG 1 cut(s) 1107
AloI GAACNNNNNNTCC 2 cut(s) 1272, 1304
Alw21I GWGCWC 2 cut(s) 691, 1133
Alw26I GTCTC 1 cut(s) 106
Alw44I GTGCAC 1 cut(s) 1129
AlwI GGATC 3 cut(s) 132, 862, 1317
AlwNI CAGNNNCTG 2 cut(s) 104, 767
Ama87I CYCGRG 1 cut(s) 501
Aor13HI TCCGGA 1 cut(s) 1841
AoxI GGCC 3 cut(s) 114, 378, 922
ApaLI GTGCAC 1 cut(s) 1129
ApeKI GCWGC 8 cut(s) 32, 347, 356, 746, 844, 863, 1210, 1265
ApoI RAATTY 8 cut(s) 757, 963, 1180, 1242, 1319, 1970, 2049, 2081
AsiGI ACCGGT 1 cut(s) 1976
AspLEI GCGC 1 cut(s) 1215
AspS9I GGNCC 5 cut(s) 337, 379, 569, 1568, 1690
AsuC2I CCSGG 2 cut(s) 734, 1502
AsuHPI GGTGA 7 cut(s) 455, 686, 791, 1270, 1625, 1646, 1966
AvaI CYCGRG 1 cut(s) 501
AvaII GGWCC 4 cut(s) 337, 569, 1568, 1690
BaeGI GKGCMC 2 cut(s) 982, 1133
BaeI ACNNNNGTAYC 2 cut(s) 1539, 1572
BalI TGGCCA 1 cut(s) 116
BauI CACGAG 2 cut(s) 1551, 2019
BbsI GAAGAC 3 cut(s) 20, 1727, 1858
Bbv12I GWGCWC 2 cut(s) 691, 1133
BbvI GCAGC 8 cut(s) 19, 334, 343, 758, 850, 856, 1222, 1252
BccI CCATC 6 cut(s) 68, 200, 574, 844, 1824, 2060
BciT130I CCWGG 1 cut(s) 875
BciVI GTATCC 1 cut(s) 307
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 2 cut(s) 734, 1502
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 4 cut(s) 975, 1806, 1857, 2007
BfoI RGCGCY 1 cut(s) 1216
BfuAI ACCTGC 2 cut(s) 1246, 1601
BfuI GTATCC 1 cut(s) 307
BglI GCCNNNNNGGC 1 cut(s) 113
BlpI GCTNAGC 1 cut(s) 360
BmcAI AGTACT 1 cut(s) 435
Bme1390I CCNGG 3 cut(s) 734, 875, 1502
Bme18I GGWCC 4 cut(s) 337, 569, 1568, 1690
BmeT110I CYCGRG 1 cut(s) 501
BmgT120I GGNCC 5 cut(s) 337, 379, 569, 1568, 1690
BmiI GGNNCC 3 cut(s) 1395, 1579, 1623
BmrFI CCNGG 3 cut(s) 734, 875, 1502
BmrI ACTGGG 1 cut(s) 1709
BmuI ACTGGG 1 cut(s) 1709
BpiI GAAGAC 3 cut(s) 20, 1727, 1858
BpmI CTGGAG 2 cut(s) 145, 1148
Bpu1102I GCTNAGC 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 458, 1057
BpuMI CCSGG 2 cut(s) 734, 1502
Bsa29I ATCGAT 1 cut(s) 642
BsaJI CCNNGG 3 cut(s) 775, 873, 1174
BsaWI WCCGGW 3 cut(s) 53, 1841, 1976
BsaXI ACNNNNNCTCC 4 cut(s) 1052, 1082, 1267, 1297
Bsc4I CCNNNNNNNGG 7 cut(s) 739, 781, 900, 1222, 1390, 1631, 1702
Bse118I RCCGGY 2 cut(s) 893, 1976
Bse1I ACTGG 7 cut(s) 162, 626, 1165, 1204, 1715, 1956, 1995
Bse3DI GCAATG 1 cut(s) 749
BseAI TCCGGA 1 cut(s) 1841
BseBI CCWGG 1 cut(s) 875
BseCI ATCGAT 1 cut(s) 642
BseDI CCNNGG 3 cut(s) 775, 873, 1174
BseGI GGATG 9 cut(s) 194, 585, 865, 1381, 1711, 1738, 1915, 2116, 2149
BseLI CCNNNNNNNGG 7 cut(s) 739, 781, 900, 1222, 1390, 1631, 1702
BseMI GCAATG 1 cut(s) 749
BseMII CTCAG 6 cut(s) 126, 351, 374, 816, 1437, 1620
BseNI ACTGG 7 cut(s) 162, 626, 1165, 1204, 1715, 1956, 1995
BseRI GAGGAG 4 cut(s) 1073, 1385, 1449, 2056
BseSI GKGCMC 2 cut(s) 982, 1133
BseXI GCAGC 8 cut(s) 19, 334, 343, 758, 850, 856, 1222, 1252
BsgI GTGCAG 1 cut(s) 1442
Bsh1285I CGRYCG 1 cut(s) 1108
BshFI GGCC 3 cut(s) 116, 380, 924
BshTI ACCGGT 1 cut(s) 1976
BshVI ATCGAT 1 cut(s) 642
BsiEI CGRYCG 1 cut(s) 1108
BsiHKAI GWGCWC 2 cut(s) 691, 1133
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 7 cut(s) 54, 733, 894, 1501, 1581, 1842, 1977
BslI CCNNNNNNNGG 7 cut(s) 739, 781, 900, 1222, 1390, 1631, 1702
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 3 cut(s) 116, 380, 924
BsoBI CYCGRG 1 cut(s) 501
Bsp1286I GDGCHC 3 cut(s) 691, 982, 1133
Bsp13I TCCGGA 1 cut(s) 1841
Bsp143I GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
Bsp1720I GCTNAGC 1 cut(s) 360
BspACI CCGC 5 cut(s) 497, 925, 1072, 1216, 1278
BspANI GGCC 3 cut(s) 116, 380, 924
BspCNI CTCAG 6 cut(s) 127, 352, 375, 817, 1438, 1621
BspDI ATCGAT 1 cut(s) 642
BspEI TCCGGA 1 cut(s) 1841
BspHI TCATGA 2 cut(s) 394, 796
BspLI GGNNCC 3 cut(s) 1395, 1579, 1623
BspMI ACCTGC 2 cut(s) 1246, 1601
BspPI GGATC 3 cut(s) 132, 862, 1317
BsrBI CCGCTC 1 cut(s) 1278
BsrDI GCAATG 1 cut(s) 749
BsrFI RCCGGY 2 cut(s) 893, 1976
BsrI ACTGG 7 cut(s) 162, 626, 1165, 1204, 1715, 1956, 1995
BssAI RCCGGY 2 cut(s) 893, 1976
BssECI CCNNGG 3 cut(s) 775, 873, 1174
BssMI GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
BssSI CACGAG 2 cut(s) 1551, 2019
Bst2BI CACGAG 2 cut(s) 1551, 2019
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 6 cut(s) 100, 409, 1126, 1175, 1418, 2074
Bst6I CTCTTC 5 cut(s) 237, 545, 732, 780, 2031
BstC8I GCNNGC 3 cut(s) 822, 1763, 1779
BstDSI CCRYGG 2 cut(s) 775, 1174
BstF5I GGATG 9 cut(s) 194, 585, 865, 1381, 1711, 1738, 1915, 2116, 2149
BstH2I RGCGCY 1 cut(s) 1216
BstHHI GCGC 1 cut(s) 1215
BstKTI GATC 9 cut(s) 127, 217, 394, 838, 857, 1108, 1156, 1312, 1618
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
BstMCI CGRYCG 1 cut(s) 1108
BstMWI GCNNNNNNNGC 8 cut(s) 32, 113, 179, 353, 356, 695, 869, 1778
BstNI CCWGG 1 cut(s) 875
BstSCI CCNGG 3 cut(s) 732, 873, 1500
BstSLI GKGCMC 2 cut(s) 982, 1133
BstV1I GCAGC 8 cut(s) 19, 334, 343, 758, 850, 856, 1222, 1252
BstV2I GAAGAC 3 cut(s) 20, 1727, 1858
Bsu15I ATCGAT 1 cut(s) 642
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 3 cut(s) 116, 380, 924
BsuTUI ATCGAT 1 cut(s) 642
BtgI CCRYGG 2 cut(s) 775, 1174
BtsCI GGATG 9 cut(s) 194, 585, 865, 1381, 1711, 1738, 1915, 2116, 2149
BtsIMutI CAGTG 8 cut(s) 169, 201, 405, 537, 949, 1131, 1423, 1949
BveI ACCTGC 2 cut(s) 1246, 1601
Cac8I GCNNGC 3 cut(s) 822, 1763, 1779
CaiI CAGNNNCTG 2 cut(s) 104, 767
CciI TCATGA 2 cut(s) 394, 796
CfoI GCGC 1 cut(s) 1215
Cfr10I RCCGGY 2 cut(s) 893, 1976
Cfr13I GGNCC 5 cut(s) 337, 379, 569, 1568, 1690
ClaI ATCGAT 1 cut(s) 642
CseI GACGC 1 cut(s) 295
Csp6I GTAC 7 cut(s) 434, 629, 832, 1548, 1988, 2070, 2104
CspAI ACCGGT 1 cut(s) 1976
CviAII CATG 9 cut(s) 37, 109, 121, 353, 395, 545, 797, 1151, 1882
CviQI GTAC 7 cut(s) 434, 629, 832, 1548, 1988, 2070, 2104
DpnI GATC 9 cut(s) 126, 216, 393, 837, 856, 1107, 1155, 1311, 1617
DpnII GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
DraIII CACNNNGTG 2 cut(s) 1128, 1687
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 5 cut(s) 237, 545, 732, 780, 2031
EarI CTCTTC 5 cut(s) 237, 545, 732, 780, 2031
Eco47I GGWCC 4 cut(s) 337, 569, 1568, 1690
Eco57I CTGAAG 5 cut(s) 414, 620, 888, 1178, 1471
Eco88I CYCGRG 1 cut(s) 501
EcoO109I RGGNCCY 2 cut(s) 337, 379
EcoRI GAATTC 2 cut(s) 757, 1970
EcoRII CCWGG 1 cut(s) 873
FaeI CATG 9 cut(s) 40, 112, 124, 356, 398, 548, 800, 1154, 1885
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FatI CATG 9 cut(s) 36, 108, 120, 352, 394, 544, 796, 1150, 1881
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 2 cut(s) 590, 1589
FokI GGATG 8 cut(s) 181, 592, 872, 1388, 1718, 1725, 1922, 2123
FspBI CTAG 4 cut(s) 975, 1806, 1857, 2007
GlaI GCGC 1 cut(s) 1214
GsuI CTGGAG 2 cut(s) 145, 1148
HaeII RGCGCY 1 cut(s) 1216
HaeIII GGCC 3 cut(s) 116, 380, 924
HapII CCGG 7 cut(s) 54, 733, 894, 1501, 1581, 1842, 1977
HgaI GACGC 1 cut(s) 295
HhaI GCGC 1 cut(s) 1215
Hin1II CATG 9 cut(s) 40, 112, 124, 356, 398, 548, 800, 1154, 1885
Hin6I GCGC 1 cut(s) 1213
HinP1I GCGC 1 cut(s) 1213
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 2 cut(s) 602, 818
HinfI GANTC 8 cut(s) 622, 764, 886, 1800, 1822, 1921, 2003, 2044
HpaII CCGG 7 cut(s) 54, 733, 894, 1501, 1581, 1842, 1977
HphI GGTGA 7 cut(s) 455, 686, 791, 1270, 1625, 1646, 1966
Hpy166II GTNNAC 7 cut(s) 51, 591, 906, 1131, 1228, 1590, 1690
Hpy8I GTNNAC 7 cut(s) 51, 591, 906, 1131, 1228, 1590, 1690
HpyCH4III ACNGT 6 cut(s) 100, 409, 1126, 1175, 1418, 2074
HpyCH4IV ACGT 1 cut(s) 325
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 113, 179, 353, 356, 695, 869, 1778
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 9 cut(s) 40, 112, 124, 356, 398, 548, 800, 1154, 1885
HspAI GCGC 1 cut(s) 1213
Kpn2I TCCGGA 1 cut(s) 1841
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
LmnI GCTCC 6 cut(s) 735, 775, 1167, 1275, 1399, 1577
Lsp1109I GCAGC 8 cut(s) 19, 334, 343, 758, 850, 856, 1222, 1252
MaeI CTAG 4 cut(s) 975, 1806, 1857, 2007
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 1997
MalI GATC 9 cut(s) 126, 216, 393, 837, 856, 1107, 1155, 1311, 1617
MbiI CCGCTC 1 cut(s) 1278
MboI GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
MfeI CAATTG 2 cut(s) 839, 1560
MhlI GDGCHC 3 cut(s) 691, 982, 1133
MlsI TGGCCA 1 cut(s) 116
MluNI TGGCCA 1 cut(s) 116
MlyI GAGTC 4 cut(s) 1794, 1816, 1997, 2053
MmeI TCCRAC 3 cut(s) 932, 1035, 1546
Mox20I TGGCCA 1 cut(s) 116
MroI TCCGGA 1 cut(s) 1841
MscI TGGCCA 1 cut(s) 116
MseI TTAA 3 cut(s) 317, 1119, 1329
MslI CAYNNNNRTG 3 cut(s) 801, 1107, 1149
Msp20I TGGCCA 1 cut(s) 116
MspA1I CMGCKG 2 cut(s) 847, 1074
MspI CCGG 7 cut(s) 54, 733, 894, 1501, 1581, 1842, 1977
MspR9I CCNGG 3 cut(s) 734, 875, 1502
MunI CAATTG 2 cut(s) 839, 1560
MvaI CCWGG 1 cut(s) 875
MwoI GCNNNNNNNGC 8 cut(s) 32, 113, 179, 353, 356, 695, 869, 1778
NciI CCSGG 2 cut(s) 734, 1502
NdeII GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
NlaIII CATG 9 cut(s) 40, 112, 124, 356, 398, 548, 800, 1154, 1885
NlaIV GGNNCC 3 cut(s) 1395, 1579, 1623
NmuCI GTSAC 1 cut(s) 1997
OliI CACNNNNGTG 1 cut(s) 1107
PaeR7I CTCGAG 1 cut(s) 501
PagI TCATGA 2 cut(s) 394, 796
PaqCI CACCTGC 2 cut(s) 1246, 1601
PfeI GAWTC 4 cut(s) 622, 764, 886, 1921
PfoI TCCNGGA 1 cut(s) 732
PinAI ACCGGT 1 cut(s) 1976
Ple19I CGATCG 1 cut(s) 1108
PleI GAGTC 4 cut(s) 1794, 1816, 1997, 2052
PpsI GAGTC 4 cut(s) 1794, 1816, 1997, 2052
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 873
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 3 cut(s) 1395, 1579, 1623
PspPI GGNCC 5 cut(s) 337, 379, 569, 1568, 1690
PspPPI RGGWCCY 1 cut(s) 337
PspXI VCTCGAGB 1 cut(s) 501
PstNI CAGNNNCTG 2 cut(s) 104, 767
PvuI CGATCG 1 cut(s) 1108
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 7 cut(s) 435, 630, 833, 1549, 1989, 2071, 2105
RsaNI GTAC 7 cut(s) 434, 629, 832, 1548, 1988, 2070, 2104
RseI CAYNNNNRTG 3 cut(s) 801, 1107, 1149
SaqAI TTAA 3 cut(s) 317, 1119, 1329
Sau3AI GATC 9 cut(s) 124, 214, 391, 835, 854, 1105, 1153, 1309, 1615
Sau96I GGNCC 5 cut(s) 337, 379, 569, 1568, 1690
ScaI AGTACT 1 cut(s) 435
SchI GAGTC 4 cut(s) 1794, 1816, 1997, 2053
ScrFI CCNGG 3 cut(s) 734, 875, 1502
SduI GDGCHC 3 cut(s) 691, 982, 1133
Sfr274I CTCGAG 1 cut(s) 501
SgrAI CRCCGGYG 1 cut(s) 1976
SinI GGWCC 4 cut(s) 337, 569, 1568, 1690
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 3 cut(s) 801, 1107, 1149
SmlI CTYRAG 3 cut(s) 437, 501, 1036
SmoI CTYRAG 3 cut(s) 437, 501, 1036
SsiI CCGC 5 cut(s) 497, 925, 1072, 1216, 1278
SspMI CTAG 4 cut(s) 975, 1806, 1857, 2007
StyD4I CCNGG 3 cut(s) 732, 873, 1500
TaaI ACNGT 6 cut(s) 100, 409, 1126, 1175, 1418, 2074
TaiI ACGT 1 cut(s) 328
TaqI TCGA 4 cut(s) 502, 642, 1032, 1312
TatI WGTACW 3 cut(s) 433, 1987, 2103
TauI GCSGC 4 cut(s) 500, 927, 1074, 1218
TfiI GAWTC 4 cut(s) 622, 764, 886, 1921
Tru1I TTAA 3 cut(s) 317, 1119, 1329
Tru9I TTAA 3 cut(s) 317, 1119, 1329
TscAI CASTG 8 cut(s) 169, 208, 412, 537, 949, 1131, 1423, 1956
TseFI GTSAC 1 cut(s) 1997
TseI GCWGC 8 cut(s) 32, 347, 356, 746, 844, 863, 1210, 1265
Tsp45I GTSAC 1 cut(s) 1997
TspGWI ACGGA 1 cut(s) 1590
TspRI CASTG 8 cut(s) 169, 208, 412, 537, 949, 1131, 1423, 1956
VneI GTGCAC 1 cut(s) 1129
VpaK11BI GGWCC 4 cut(s) 337, 569, 1568, 1690
XapI RAATTY 8 cut(s) 757, 963, 1180, 1242, 1319, 1970, 2049, 2081
XbaI TCTAGA 1 cut(s) 2006
XcmI CCANNNNNNNNNTGG 1 cut(s) 1172
XhoI CTCGAG 1 cut(s) 501
XmiI GTMKAC 2 cut(s) 590, 1589
XspI CTAG 4 cut(s) 975, 1806, 1857, 2007
ZrmI AGTACT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.