Rorug04G0201000

Palmitoyl-monogalactosyldiacylglycerol delta-7 desaturase, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000059
Physical Location & Seq
Reverse (-)
34898027 .. 34913340
15314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0201000.1

Sequence Viewer

Length: 3951 bp
ATGAGTACGGTAGACAAGATGCTTATAAAGGGAATCCGGAGCTTCGACCCGGAGAACAAGCACGTCATCACCTTCTTCAAACCCCTAACCCTAATCGTCGGCCACAACGGCGCCGGAAAAACCACCATCATCGAGTGCCTCAAGCTCTCTTGCACCGGCGAGTTGCCTCCCAATGCCAGGTCCGGCCACAGCTTCATCCACGACCCCAAAGTCGCCGGCGAAACCGAGACCAAAGGTCAGATTAAGCTCCGCTTCAAGACGGCCGCCGGCAAGGACGTCGTCTCTATCAGGTCGTTTCAGCTCACTCAGAAAGCCTCCAAAATGGAGTATAAGGCCATAGAGAGCGTCCTTCAAACTATTAATCCCCACACCGGAGAGAAAGTTTGCCTTAGCTATAGATGTGCTGACATGGATAAGGAAATCCCTGCTTTGATGGGTGTCTCGAAGGCGATTCTCGAAAATGTGATCTTTGCTCACCAAGATGAAGCCAATTGGCCTTTGCAGGATCCTTCCACACTGAAGAAGAAGTTTGATGATATCTTCTCTGCTACACGGTATACCAAGGCATTGGAGGTAATTAAAAAACTTCACAAGGATCAAGCTCAAGAGATAAAGACCTACAAGCTAAAATTGGAGAATCTTCAAACCTTGAAAGATGCTGCATATAAGTTTCGTGAAAGCATTGCTAAAGATGAGGAAAGAACAGAATTAGTGAAAAGACAGATGCAGGAGTTGGAGGGCAGTGTCCATGATCTGGATTCTAAAATTCATAATGCTAGAGAAACACTGAAGCATCTGCGGAACCTGGATGGCCAGCTATCTGGAAAGACTGCTGAAAGAAAAACTCTAGTTAAGGAGGTGGAGAGACAGTATAGAGATCTTGCAGAAGAAAATGAAGACACTGATGAACAATTGATGGAATGGAAAACTACATTTGAAGACAGAATTCGAGAGTTAGGAACTAAAATTCGTAGATTGGAGAGGGAACAGGATGACACAAAGACCAAAAGTAAGGTCCTTACGCAGACAATAAAGGATTCTATACGGCAGATTAGCAGGCTTGAAAATGAAGCTGAGGCTCATTTGGAATTAAAGAAAGTGAGAGATTCAACCATTCAAAGTTTTTTTGCAAGGCACAATTTAGGTTCTCTTCCAAATCCCCCCTTCAGCAATGAAGTTGCTATAAACTTGACAAATAGAATAAAATCAAGGTTGTGTGATCTAGAAAAGGATTTGCAAGATAAGAAGAATTCAAATGAAACTGAAGTCAAGAAAGCATTGGACCTCTACATGGATGCAAATGATCGTTGGAAATTAAAAGAGGCTCAGCACCAGGCTAAAGCAGAAATAAAGAATGGCCTTTTGAAGCGTATTGAGGAGAAGAAACAGGAGCGTGATTCTTTTGAAAGAGATGTTTTAAACTGTAACCTTTCTCGCATTGATGAGAAAGAAAAAAACATGCGAATTGAGGTTGACAGAAAGGCAAATCAGCTTGCTGTTAGAGACTTTGATTCAACCATTCGTCAAAAGCAGAGTGAGGTGTTTAGCATTGACCAAATGATCACTGCAGTTACTCGAGAGAAGAATGTCTTGGATGGTGATAGGGATGACAGAATAATACTGTCTCACAAGAAGACAGACTTGGAGACTCAGAAAAGGAAACACAGAAAGATAATTGATGACTACAAGGACAGAATAAGAGGAGTGCTAAAAGGAAGGCTTCCTCCTGACAAGGATTTGAAAAGTGAAATCACTCAGGCCTTGAGGGTTGTTACCATGGAGTTTGATGACTTGAGCGCCAAATCTCGTGAAGTGGAGAAAGAGGTGAATATGTTTCAGATGAAAATACAAGAAGTTAATAATAACCTCTGTAAACACCGTAAAGATCTGGAATCAAAGAGGAGATATATTGAAACTAGACTTCAGGCCTTAGATCAGCAATGTTTTACTGTTGATTCCTATACCAAAGTCTTGGATTCAGCAAAGGAGAAAAGAGATGTGCATAAAAGCAAATACAACTTTGCAGATGGTATGCGACAGATGTTTGATCCTTTTGAAAGAGTTGCTCGTGCCCATCATATTTGTCCTTGTTGTGAACGCCCTTTCTCTCCAGAAGAGGAAGACGAGTTCGTTAAAAAGCAAAAAGTGAAGGCAGCAACTTCTTCTGAACAAATAAAAGCACTCTTAGTGGAATCCTCAAGTGCTGACTCTTTTTTTCAGCAAGTGGACAAGCTTCGCTTGTTTTATGAAGAATATATAAAAATAGGCAAGGAAGCAATCCCTAATGCAGAGAAAGAACTATCTGAGCTTAGTGAAGAGATGGAACAAAAATCCCAAGCCCTTGATGATGTGTTGGCTGTTTCTGCTCAAGTAAAGTCTGATAAGGACTTGATTGAGGCCTTGGTGCAACCCATTGAAACTGCTGATAGACTACTTCAAGAAATACAAATACGCCAGAAGGACGTTGATGACTTGGAGTATAAGCTTGATATTCAAGGGCCAGGTGCAAGATCCTTGGAGGATATTCAGTTAGAGCTGACTAATCTGCAAAGAAAAAAGGACAGTTTGCATGGTGAACTAGAGAAATTAAGAGAGGAACAAAGGTACATGGAAGGTGATTTATCAAACATTCAAATGCGGTGGCACAGTGTAAGGGAAGAGAAAATCAATGCCACTACTATGTTTCGTGATTTTGAAAGGACAGAAGAAGAGTTAGAGCGTTTGACCGATGAAAAGAGTCAAATTGATCTTGATGAAAAGCTTCTGGCAGAGGCTCTTGGCCCTTTATCAAGGGAGAAAGATAAATTTCTTAATGACTACAATGAAATTAAAGCTGAACTCGGTCGCCAGTGTGAGGAGCAAGCTGAACAAAGCAGAAATTACAAGCACAAAGTTGATGAACTACTTAAGATTATTTCTGACATTAAAGAGTATAATGATTTAAGGAAAGGAGAGAGGTTGCAGGAACTGCAAGAGAAGCAACGTCTATCAGAATCTCAACTTCAAAGTTGTGATACTAGACTGCAGGAAATTTCGGATGAATTAGGCAGAAGTAATAAGTTAATGGAGGGTCAAGAGGAACTAAGGCGAAATATAGATGCCAATTTGAATTACAGGAAAACCAAAGCTGAAATAGATCAACTTACTCAAGAGGTCGTATCACTCGAAGCAGAAATCCTTAAATTCGGTGAAATTCCCAAGTGTGAAGCTCAACTCGCAAAGCTCTCACAGGAGAGAGAGAGGCTTCTTTCAGAGTTAAACAAGTTTCGTGGAACAATATCGGTTTACCAGAGCAATATTTCAAATTATAAAATTGATCTCAAACAGGCACAATACAAGGACATTGACAAACGGTACTTTGATCAGCTTATCCAGCTAAAGACATCTGAGATGGCAAACAAGGACCTGGACAGATATTACAATGCGCTTGACAAAGCACTGATGCGCTTCCACTCGATGAAGATGGAGGAAATAAATAAGATTATACGGGAGTTGTGGCAGCAGACTTACAGAGGACAAGATATAGATTATATCTGCATACATTCAGATTCTGAAAGTGCTGGGACTCGTTCATATAGCTACAAGGTTCTCATGCAAACTGGCGATACAGAACTAGAAATGAGAGGAAGATGTAGTGCTGGTCAGAAGGTCCTTGCTTCCCTGATCATAAGGTTGGCTTTAGCTGAAACTTTTTGCCTCAATTGTGGAATACTAGCACTAGATGAACCAACTACAAACTTGGACGGACCAAATGCGGAGAGTCTTGCTGCTGCTCTTGTTAGAATTATGGAGGACCGGAAAGGGCAGGAGAACTTTCAGTTGATAGTAATTACTCATGATGAGCGCTTTGCTCAACTAATTGGCCAGCGCCAGCATGCAGAAAAATATTATCGGGTGACAAAGGACGATCACCAGCATAGCATAATTGAAGCCCAGGAGATTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko01040 ko01212 ko03320 ko04152 ko04212 map01040 map01212 map03320 map04152 map04212
Pfam Domains
Protein Families
FA_desaturase

Protein Analysis

1316

Amino Acids

153.17

Weight (kDa)

5.99

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMC_N PF02463 4 - 105 4.8e-06 RecF/RecN/SMC N terminal domain
AAA_23 PF13476 6 - 215 1.1e-33 AAA domain
Rad50_zn_hook PF04423 688 - 724 4.7e-06 Rad50 zinc hook motif
SMC_N PF02463 1076 - 1302 6.7e-09 RecF/RecN/SMC N terminal domain
SbcC_Walker_B PF13558 1190 - 1259 2.9e-06 SbcC/RAD50-like, Walker B motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06080 AT1G06080 AT2G31360 AT2G31360
fragaria_vesca FvH4_1g04480 FvH4_1g04480 FvH4_1g04480 FvH4_1g04480 FvH4_3g00350 FvH4_3g28040 FvH4_3g28040 FvH4_3g28040 FvH4_3g28050 FvH4_5g34260 FvH4_6g42131 FvH4_6g42140 FvH4_6g42171 FvH4_6g42171 FvH4_6g42171 FvH4_6g42171 FvH4_6g42171 FvH4_6g42311 FvH4_6g42312 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640 FvH4_6g43640
malus_domestica MD00G1098700.v1.1 MD00G1139800.v1.1 MD00G1174400.v1.1 MD02G1133600.v1.1 MD02G1271200.v1.1 MD09G1114000.v1.1 MD09G1114100.v1.1 MD09G1114200.v1.1 MD11G1194300.v1.1 MD15G1438800.v1.1 MD17G1105800.v1.1 MD17G1105900.v1.1
prunus_persica Prupe.3G210300_v2.0.a1 Prupe.3G210300_v2.0.a1 Prupe.3G210400_v2.0.a1 Prupe.3G210500_v2.0.a1 Prupe.3G210600_v2.0.a1 Prupe.3G210600_v2.0.a1 Prupe.4G228700_v2.0.a1 Prupe.4G228800_v2.0.a1 Prupe.4G228900_v2.0.a1 Prupe.4G229000_v2.0.a1 Prupe.4G229300_v2.0.a1 Prupe.4G229500_v2.0.a1 Prupe.4G229600_v2.0.a1 Prupe.4G259100_v2.0.a1 Prupe.4G259400_v2.0.a1 Prupe.4G259500_v2.0.a1 Prupe.4G259600_v2.0.a1 Prupe.4G259600_v2.0.a1 Prupe.4G259600_v2.0.a1 Prupe.4G259800_v2.0.a1 Prupe.4G259900_v2.0.a1
pyrus_communis pycom03g12440 pycom09g03830 pycom09g03840 pycom09g03850 pycom11g16300 pycom15g38680 pycom17g09850
rosa_chinensis RchiOBHm_Chr1g0337221 RchiOBHm_Chr1g0343481 RchiOBHm_Chr1g0343491 RchiOBHm_Chr1g0343531 RchiOBHm_Chr1g0343551 RchiOBHm_Chr1g0343711 RchiOBHm_Chr1g0343721 RchiOBHm_Chr1g0343731 RchiOBHm_Chr1g0360301 RchiOBHm_Chr2g0086981 RchiOBHm_Chr2g0142871 RchiOBHm_Chr2g0156531 RchiOBHm_Chr2g0157491 RchiOBHm_Chr2g0157791 RchiOBHm_Chr2g0158051 RchiOBHm_Chr2g0158061 RchiOBHm_Chr2g0158141 RchiOBHm_Chr2g0158151 RchiOBHm_Chr2g0158221 RchiOBHm_Chr2g0158251 RchiOBHm_Chr2g0159451 RchiOBHm_Chr4g0403611 RchiOBHm_Chr4g0412621 RchiOBHm_Chr4g0424961 RchiOBHm_Chr4g0425001 RchiOBHm_Chr4g0425091 RchiOBHm_Chr5g0012311 RchiOBHm_Chr5g0022121 RchiOBHm_Chr5g0050081 RchiOBHm_Chr5g0050721 RchiOBHm_Chr5g0050731 RchiOBHm_Chr5g0050761 RchiOBHm_Chr6g0252581 RchiOBHm_Chr7g0199141 RchiOBHm_Chr7g0216811 RchiOBHm_Chr7g0234331 RchiOBHm_Chr7g0234341 RchiOBHm_Chr7g0234371 RchiOBHm_Chr7g0234431 RchiOBHm_Chr7g0234681 RchiOBHm_Chr7g0234741
rosa_laevigata RLG00000001215 RLG00000001222 RLG00000001223 RLG00000001229 RLG00000003811 RLG00000007410 RLG00000015084 RLG00000017931 RLG00000020899 RLG00000020919 RLG00000020994 RLG00000021020 RLG00000021055 RLG00000021056 RLG00000021057 RLG00000021063 RLG00000021065 RLG00000021066 RLG00000021069 RLG00000021071 RLG00000021156 RLG00000024999 RLG00000025005 RLG00000028964 RLG00000029292 RLG00000032646 RLG00000034725 RLG00000034726 RLG00000034730
rosa_multiflora Rmu_co8371849.1_g000001 Rmu_co8422897.1_g000001 Rmu_sc0000195.1_g000039 Rmu_sc0000333.1_g000003 Rmu_sc0000419.1_g000030 Rmu_sc0000419.1_g000034 Rmu_sc0002016.1_g000040 Rmu_sc0002117.1_g000018 Rmu_sc0002117.1_g000023 Rmu_sc0002160.1_g000002 Rmu_sc0002204.1_g000008 Rmu_sc0002775.1_g000001 Rmu_sc0002775.1_g000013 Rmu_sc0002801.1_g000001 Rmu_sc0002801.1_g000013 Rmu_sc0004434.1_g000003 Rmu_sc0004864.1_g000019 Rmu_sc0006966.1_g000006 Rmu_sc0007763.1_g000002 Rmu_sc0007769.1_g000006 Rmu_sc0007769.1_g000013 Rmu_sc0008904.1_g000001 Rmu_sc0013250.1_g000001 Rmu_sc0013327.1_g000004 Rmu_sc0037251.1_g000001 Rmu_ssc0000217.1_g000013 Rmu_ssc0000420.1_g000014 Rmu_ssc0000420.1_g000016
rosa_roxburghii Rroxscaffold_1G00030000 Rroxscaffold_1G00030030 Rroxscaffold_1G00030060 Rroxscaffold_2G00090560 Rroxscaffold_2G00090580 Rroxscaffold_2G00091590 Rroxscaffold_2G00091630 Rroxscaffold_2G00091670 Rroxscaffold_2G00091700 Rroxscaffold_2G00091950 Rroxscaffold_2G00092160 Rroxscaffold_2G00132010 Rroxscaffold_3G00226740 Rroxscaffold_3G00226760 Rroxscaffold_5G00356990 Rroxscaffold_5G00367150 Rroxscaffold_5G00368290 Rroxscaffold_7G00212540
rosa_rugosa Rorug01G0132700.1 Rorug01G0132800.1 Rorug01G0132900.1 Rorug01G0133000.1 Rorug01G0133100.1 Rorug01G0133200.1 Rorug01G0167200 Rorug01G0471200 Rorug02G0461800 Rorug02G0463400 Rorug02G0463500 Rorug02G0463600 Rorug02G0463700 Rorug02G0463700 Rorug02G0463700 Rorug02G0465500 Rorug02G0465800 Rorug02G0465900.1 Rorug02G0466000 Rorug02G0466100 Rorug02G0466200 Rorug02G0466300 Rorug02G0466600 Rorug02G0475900 Rorug04G0200800 Rorug04G0201000 Rorug04G0201100 Rorug04G0384700 Rorug05G0000500 Rorug05G0257600 Rorug05G0257600 Rorug05G0263300 Rorug05G0263700 Rorug05G0537100 Rorug05G0579200 Rorug05G0579200 Rorug07G0155500 Rorug07G0163100 Rorug07G0283400 Rorug07G0284800 Rorug07G0285400
rosa_samantha Rh1AG184700 Rh1BG152200 Rh1DG184100 Rh2AG024000 Rh2AG525800 Rh2AG529100 Rh2AG531600 Rh2AG531800 Rh2AG532600 Rh2AG532700 Rh2AG542500 Rh2BG024600 Rh2BG540500 Rh2BG543000 Rh2BG546000 Rh2BG546100 Rh2BG546500 Rh2BG555700 Rh2CG024700 Rh2CG510500 Rh2CG513400 Rh2CG515800 Rh2CG515900 Rh2CG516300 Rh2CG516400 Rh2CG525100 Rh2CG525900 Rh2DG549000 Rh2DG551900 Rh2DG554300 Rh2DG554500 Rh2DG555100 Rh2DG564800 Rh2DG565100 Rh4AG174400 Rh4AG258000 Rh4BG263600 Rh4CG274800 Rh4DG170100 Rh4DG259200 Rh4DG259500 Rh5AG094500 Rh5AG159500 Rh5AG330300 Rh5AG335300 Rh5AG335400 Rh5AG335800 Rh5BG090700 Rh5BG159000 Rh5BG341000 Rh5BG344600 Rh5BG344800 Rh5BG345200 Rh5CG173200 Rh5CG372600 Rh5CG372700 Rh5CG373100 Rh5DG090200 Rh5DG159000 Rh5DG353800 Rh5DG357700 Rh5DG357900 Rh5DG358300 Rh6AG053400 Rh7AG176500 Rh7AG301900 Rh7AG439800 Rh7BG294700 Rh7BG412800 Rh7CG185600 Rh7CG320600 Rh7CG460400 Rh7CG460500 Rh7CG460900 Rh7CG461300 Rh7DG179500 Rh7DG303400 Rh7DG429500 Rh7DG429600
rosa_wichuraiana Rw0G019480 Rw1G012510 Rw1G015180 Rw2G001890 Rw2G043550 Rw2G043740 Rw2G043960 Rw2G043970 Rw2G044020 Rw2G044030 Rw2G044080 Rw2G044100 Rw2G044850 Rw2G044910 Rw4G014610 Rw4G022270 Rw4G022280 Rw4G022310 Rw5G008250 Rw5G014230 Rw5G031190 Rw5G031640 Rw5G031650 Rw5G031680 Rw6G004800 Rw7G015520 Rw7G025750 Rw7G036370 Rw7G036390 Rw7G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 26, 3309
AasI GACNNNNNNGTC 1 cut(s) 209
AatII GACGTC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 754
AccI GTMKAC 2 cut(s) 12, 557
AccIII TCCGGA 1 cut(s) 36
AciI CCGC 5 cut(s) 250, 264, 799, 2638, 3755
AclWI GGATC 5 cut(s) 500, 513, 603, 2042, 2505
AcoI YGGCCR 5 cut(s) 100, 184, 261, 811, 3862
AcsI RAATTY 8 cut(s) 765, 945, 966, 1249, 2804, 3030, 3182, 3192
AcuI CTGAAG 5 cut(s) 539, 809, 1150, 1284, 1907
AcyI GRCGYC 2 cut(s) 111, 276
AfaI GTAC 3 cut(s) 7, 2606, 3356
AfeI AGCGCT 1 cut(s) 3845
AfiI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
AflII CTTAAG 1 cut(s) 2906
AhdI GACNNNNNGTC 1 cut(s) 234
AjiI CACGTC 1 cut(s) 64
AjnI CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
AjuI GAANNNNNNNTTGG 4 cut(s) 1574, 1606, 1625, 1657
AloI GAACNNNNNNTCC 2 cut(s) 2111, 2143
Alw26I GTCTC 7 cut(s) 221, 286, 445, 859, 1497, 1629, 1640
AlwI GGATC 5 cut(s) 500, 513, 603, 2042, 2505
AlwNI CAGNNNCTG 2 cut(s) 2968, 3551
Ama87I CYCGRG 1 cut(s) 1575
Aor13HI TCCGGA 1 cut(s) 36
Aor51HI AGCGCT 1 cut(s) 3845
ApeKI GCWGC 5 cut(s) 659, 2153, 3499, 3767, 3770
ApoI RAATTY 8 cut(s) 765, 945, 966, 1249, 2804, 3030, 3182, 3192
ArsI GACNNNNNNTTYG 2 cut(s) 999, 1031
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 2760
AspLEI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
AspS9I GGNCC 9 cut(s) 180, 1015, 1282, 2498, 2780, 3403, 3649, 3746, 3793
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 9 cut(s) 61, 467, 1610, 1837, 2585, 2627, 3200, 3902, 3907
AvaI CYCGRG 1 cut(s) 1575
AvaII GGWCC 7 cut(s) 180, 1015, 1282, 3403, 3649, 3746, 3793
BaeGI GKGCMC 1 cut(s) 2074
BalI TGGCCA 2 cut(s) 813, 3864
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 110
BauI CACGAG 2 cut(s) 1806, 2067
BbsI GAAGAC 4 cut(s) 903, 945, 1640, 2127
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
BceAI ACGGC 3 cut(s) 124, 276, 1061
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
BclI TGATCA 3 cut(s) 1560, 3361, 3663
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 7 cut(s) 221, 286, 445, 859, 1497, 1629, 1640
BfaI CTAG 9 cut(s) 777, 848, 1223, 1917, 2579, 3018, 3614, 3713, 3719
BfmI CTRYAG 3 cut(s) 394, 1566, 3023
BfoI RGCGCY 4 cut(s) 114, 1800, 3847, 3871
BfrI CTTAAG 1 cut(s) 2906
BglI GCCNNNNNGGC 1 cut(s) 108
BglII AGATCT 2 cut(s) 877, 1885
BisI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
BlpI GCTNAGC 1 cut(s) 1326
BlsI GCNGC 6 cut(s) 265, 661, 2155, 3501, 3769, 3772
Bme1390I CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
Bme18I GGWCC 7 cut(s) 180, 1015, 1282, 3403, 3649, 3746, 3793
BmeRI GACNNNNNGTC 1 cut(s) 234
BmeT110I CYCGRG 1 cut(s) 1575
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 9 cut(s) 180, 1015, 1282, 2498, 2780, 3403, 3649, 3746, 3793
BmiI GGNNCC 3 cut(s) 112, 507, 803
BmrFI CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
BmsI GCATC 7 cut(s) 9, 646, 714, 802, 1285, 3088, 3432
BpiI GAAGAC 4 cut(s) 903, 945, 1640, 2127
BpmI CTGGAG 1 cut(s) 2094
Bpu10I CCTNAGC 2 cut(s) 389, 1074
Bpu1102I GCTNAGC 1 cut(s) 1326
BpuEI CTTGAG 7 cut(s) 125, 588, 1783, 1813, 2182, 2352, 3132
BpuMI CCSGG 1 cut(s) 50
BsaHI GRCGYC 2 cut(s) 111, 276
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 5 cut(s) 561, 1776, 2400, 2514, 3933
BsaWI WCCGGW 3 cut(s) 36, 371, 3795
BsaXI ACNNNNNCTCC 4 cut(s) 366, 396, 854, 884
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
Bse118I RCCGGY 3 cut(s) 155, 215, 266
Bse1I ACTGG 2 cut(s) 2848, 3604
Bse3DI GCAATG 3 cut(s) 681, 1177, 1946
BseAI TCCGGA 1 cut(s) 36
BseBI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
BseDI CCNNGG 5 cut(s) 561, 1776, 2400, 2514, 3933
BseGI GGATG 7 cut(s) 195, 814, 997, 1300, 1600, 1612, 3043
BseLI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
BseMI GCAATG 3 cut(s) 681, 1177, 1946
BseMII CTCAG 7 cut(s) 320, 1065, 1340, 1664, 1769, 2295, 3378
BseNI ACTGG 2 cut(s) 2848, 3604
BseRI GAGGAG 4 cut(s) 1391, 1716, 1915, 2870
BseSI GKGCMC 1 cut(s) 2074
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
BseYI CCCAGC 1 cut(s) 3560
Bsh1285I CGRYCG 2 cut(s) 264, 2845
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 2 cut(s) 264, 2845
BsiHKCI CYCGRG 1 cut(s) 1575
BsiSI CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3796
BslFI GGGAC 1 cut(s) 3577
BslI CCNNNNNNNGG 5 cut(s) 177, 371, 754, 1291, 2854
BsmAI GTCTC 7 cut(s) 221, 286, 445, 859, 1497, 1629, 1640
BsmBI CGTCTC 1 cut(s) 286
BsmFI GGGAC 1 cut(s) 3577
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 1575
Bsp1286I GDGCHC 1 cut(s) 2074
Bsp13I TCCGGA 1 cut(s) 36
Bsp1720I GCTNAGC 1 cut(s) 1326
Bsp19I CCATGG 1 cut(s) 1776
BspACI CCGC 5 cut(s) 250, 264, 799, 2638, 3755
BspCNI CTCAG 7 cut(s) 319, 1066, 1339, 1663, 1768, 2296, 3379
BspEI TCCGGA 1 cut(s) 36
BspHI TCATGA 1 cut(s) 3835
BspLI GGNNCC 3 cut(s) 112, 507, 803
BspMAI CTGCAG 2 cut(s) 1570, 3027
BspPI GGATC 5 cut(s) 500, 513, 603, 2042, 2505
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 2906
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 3 cut(s) 681, 1177, 1946
BsrFI RCCGGY 3 cut(s) 155, 215, 266
BsrI ACTGG 2 cut(s) 2848, 3604
BssAI RCCGGY 3 cut(s) 155, 215, 266
BssECI CCNNGG 5 cut(s) 561, 1776, 2400, 2514, 3933
BssNAI GTATAC 1 cut(s) 558
BssNI GRCGYC 2 cut(s) 111, 276
BssSI CACGAG 2 cut(s) 1806, 2067
BssT1I CCWWGG 4 cut(s) 561, 1776, 2400, 2514
Bst1107I GTATAC 1 cut(s) 558
Bst2BI CACGAG 2 cut(s) 1806, 2067
Bst2UI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
Bst6I CTCTTC 5 cut(s) 1155, 2109, 2310, 2652, 2703
BstACI GRCGYC 2 cut(s) 111, 276
BstAFI CTTAAG 1 cut(s) 2906
BstAPI GCANNNNNTGC 1 cut(s) 2968
BstC8I GCNNGC 9 cut(s) 217, 268, 815, 1058, 1494, 2862, 3866, 3872, 3876
BstDSI CCRYGG 1 cut(s) 1776
BstF5I GGATG 7 cut(s) 195, 814, 997, 1300, 1600, 1612, 3043
BstH2I RGCGCY 4 cut(s) 114, 1800, 3847, 3871
BstHHI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
BstMAI GTCTC 7 cut(s) 221, 286, 445, 859, 1497, 1629, 1640
BstMCI CGRYCG 2 cut(s) 264, 2845
BstMWI GCNNNNNNNGC 6 cut(s) 108, 2363, 2968, 2977, 3215, 3373
BstNI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
BstNSI RCATGY 2 cut(s) 1462, 3878
BstSCI CCNGG 7 cut(s) 48, 176, 804, 1332, 2500, 3405, 3933
BstSFI CTRYAG 3 cut(s) 394, 1566, 3023
BstSLI GKGCMC 1 cut(s) 2074
BstV1I GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
BstV2I GAAGAC 4 cut(s) 903, 945, 1640, 2127
BstX2I RGATCY 4 cut(s) 505, 877, 1885, 2510
BstXI CCANNNNNNTGG 3 cut(s) 568, 821, 1972
BstYI RGATCY 4 cut(s) 505, 877, 1885, 2510
BstZ17I GTATAC 1 cut(s) 558
BstZI CGGCCG 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 1776
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 7 cut(s) 195, 814, 997, 1300, 1600, 1612, 3043
BtsI GCAGTG 2 cut(s) 748, 1563
BtsIMutI CAGTG 8 cut(s) 515, 748, 785, 900, 1563, 2653, 2855, 3437
Cac8I GCNNGC 9 cut(s) 217, 268, 815, 1058, 1494, 2862, 3866, 3872, 3876
CaiI CAGNNNCTG 2 cut(s) 2968, 3551
CciI TCATGA 1 cut(s) 3835
CfoI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
Cfr10I RCCGGY 3 cut(s) 155, 215, 266
Cfr13I GGNCC 9 cut(s) 180, 1015, 1282, 2498, 2780, 3403, 3649, 3746, 3793
CseI GACGC 1 cut(s) 334
Csp6I GTAC 3 cut(s) 6, 2605, 3355
CspCI CAANNNNNGTGG 4 cut(s) 2621, 2656, 3250, 3285
CviQI GTAC 3 cut(s) 6, 2605, 3355
DinI GGCGCC 1 cut(s) 112
DraI TTTAAA 1 cut(s) 1419
DrdI GACNNNNNNGTC 1 cut(s) 209
DriI GACNNNNNGTC 1 cut(s) 234
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 5 cut(s) 100, 184, 261, 811, 3862
EagI CGGCCG 1 cut(s) 261
Eam1104I CTCTTC 5 cut(s) 1155, 2109, 2310, 2652, 2703
Eam1105I GACNNNNNGTC 1 cut(s) 234
EarI CTCTTC 5 cut(s) 1155, 2109, 2310, 2652, 2703
EclXI CGGCCG 1 cut(s) 261
Eco130I CCWWGG 4 cut(s) 561, 1776, 2400, 2514
Eco147I AGGCCT 3 cut(s) 1760, 1928, 2399
Eco31I GGTCTC 1 cut(s) 221
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 7 cut(s) 180, 1015, 1282, 3403, 3649, 3746, 3793
Eco47III AGCGCT 1 cut(s) 3845
Eco52I CGGCCG 1 cut(s) 261
Eco57I CTGAAG 5 cut(s) 539, 809, 1150, 1284, 1907
Eco88I CYCGRG 1 cut(s) 1575
EcoO109I RGGNCCY 3 cut(s) 1015, 3403, 3649
EcoRI GAATTC 2 cut(s) 945, 1249
EcoRII CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
EcoRV GATATC 1 cut(s) 538
EcoT14I CCWWGG 4 cut(s) 561, 1776, 2400, 2514
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 4 cut(s) 561, 1776, 2400, 2514
Esp3I CGTCTC 1 cut(s) 286
FaqI GGGAC 1 cut(s) 3577
FbaI TGATCA 3 cut(s) 1560, 3361, 3663
FblI GTMKAC 2 cut(s) 12, 557
Fnu4HI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
FokI GGATG 7 cut(s) 182, 821, 1004, 1307, 1607, 1619, 3050
Fsp4HI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
FspBI CTAG 9 cut(s) 777, 848, 1223, 1917, 2579, 3018, 3614, 3713, 3719
GlaI GCGC 6 cut(s) 112, 1798, 3426, 3446, 3845, 3869
GluI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
GsaI CCCAGC 1 cut(s) 3564
GsuI CTGGAG 1 cut(s) 2094
HaeII RGCGCY 4 cut(s) 114, 1800, 3847, 3871
HapII CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3796
HgaI GACGC 1 cut(s) 334
HhaI GCGC 6 cut(s) 113, 1799, 3427, 3447, 3846, 3870
Hin1I GRCGYC 2 cut(s) 111, 276
Hin6I GCGC 6 cut(s) 111, 1797, 3425, 3445, 3844, 3868
HinP1I GCGC 6 cut(s) 111, 1797, 3425, 3445, 3844, 3868
HincII GTYRAC 1 cut(s) 1474
HindII GTYRAC 1 cut(s) 1474
HindIII AAGCTT 3 cut(s) 2231, 2483, 2759
HpaII CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3796
HphI GGTGA 9 cut(s) 61, 467, 1610, 1837, 2585, 2627, 3200, 3902, 3907
Hpy166II GTNNAC 8 cut(s) 13, 558, 1474, 1874, 2096, 2227, 2576, 3286
Hpy8I GTNNAC 8 cut(s) 13, 558, 1474, 1874, 2096, 2227, 2576, 3286
Hpy99I CGWCG 2 cut(s) 101, 281
HpyCH4IV ACGT 4 cut(s) 63, 276, 2463, 2983
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 2363, 2968, 2977, 3215, 3373
HpySE526I ACGT 4 cut(s) 63, 276, 2463, 2983
Hsp92I GRCGYC 2 cut(s) 111, 276
HspAI GCGC 6 cut(s) 111, 1797, 3425, 3445, 3844, 3868
KasI GGCGCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 36
KroI GCCGGC 2 cut(s) 215, 266
KroNI GCCGGC 2 cut(s) 217, 268
Ksp22I TGATCA 3 cut(s) 1560, 3361, 3663
LmnI GCTCC 4 cut(s) 39, 252, 1390, 2857
Lsp1109I GCAGC 5 cut(s) 646, 2165, 3511, 3754, 3757
LweI GCATC 7 cut(s) 9, 646, 714, 802, 1285, 3088, 3432
MaeI CTAG 9 cut(s) 777, 848, 1223, 1917, 2579, 3018, 3614, 3713, 3719
MaeII ACGT 4 cut(s) 63, 276, 2463, 2983
MaeIII GTNAC 4 cut(s) 1424, 1570, 1771, 3895
MfeI CAATTG 3 cut(s) 490, 911, 3700
MflI RGATCY 4 cut(s) 505, 877, 1885, 2510
MhlI GDGCHC 1 cut(s) 2074
MlsI TGGCCA 2 cut(s) 813, 3864
MluNI TGGCCA 2 cut(s) 813, 3864
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 5 cut(s) 1642, 2201, 2746, 3559, 3769
MmeI TCCRAC 2 cut(s) 714, 1289
Mox20I TGGCCA 2 cut(s) 813, 3864
MreI CGCCGGCG 1 cut(s) 215
MroI TCCGGA 1 cut(s) 36
MroNI GCCGGC 2 cut(s) 215, 266
MroXI GAANNNNTTC 1 cut(s) 2760
MscI TGGCCA 2 cut(s) 813, 3864
MslI CAYNNNNRTG 3 cut(s) 480, 2633, 2678
Msp20I TGGCCA 2 cut(s) 813, 3864
MspCI CTTAAG 1 cut(s) 2906
MspI CCGG 9 cut(s) 37, 50, 114, 156, 183, 216, 267, 372, 3796
MspR9I CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
MunI CAATTG 3 cut(s) 490, 911, 3700
MvaI CCWGG 6 cut(s) 178, 806, 1334, 2502, 3407, 3935
MwoI GCNNNNNNNGC 6 cut(s) 108, 2363, 2968, 2977, 3215, 3373
NaeI GCCGGC 2 cut(s) 217, 268
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 1776
NgoMIV GCCGGC 2 cut(s) 215, 266
NlaIV GGNNCC 3 cut(s) 112, 507, 803
NmuCI GTSAC 1 cut(s) 3895
NspI RCATGY 2 cut(s) 1462, 3878
PaeI GCATGC 1 cut(s) 3878
PaeR7I CTCGAG 1 cut(s) 1575
PagI TCATGA 1 cut(s) 3835
PceI AGGCCT 3 cut(s) 1760, 1928, 2399
PcsI WCGNNNNNNNCGW 1 cut(s) 3162
PdiI GCCGGC 2 cut(s) 217, 268
PdmI GAANNNNTTC 1 cut(s) 2760
PflFI GACNNNGTC 1 cut(s) 278
PflMI CCANNNNNTGG 1 cut(s) 754
PkrI GCNGC 6 cut(s) 265, 661, 2155, 3501, 3769, 3772
PleI GAGTC 5 cut(s) 1642, 2201, 2745, 3559, 3768
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 5 cut(s) 1642, 2201, 2745, 3559, 3768
PpuMI RGGWCCY 3 cut(s) 1015, 3403, 3649
PshBI ATTAAT 1 cut(s) 360
PsiI TTATAA 2 cut(s) 26, 3309
Psp5II RGGWCCY 3 cut(s) 1015, 3403, 3649
Psp6I CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
PspFI CCCAGC 1 cut(s) 3560
PspGI CCWGG 6 cut(s) 176, 804, 1332, 2500, 3405, 3933
PspN4I GGNNCC 3 cut(s) 112, 507, 803
PspPI GGNCC 9 cut(s) 180, 1015, 1282, 2498, 2780, 3403, 3649, 3746, 3793
PspPPI RGGWCCY 3 cut(s) 1015, 3403, 3649
PstI CTGCAG 2 cut(s) 1570, 3027
PstNI CAGNNNCTG 2 cut(s) 2968, 3551
PsuI RGATCY 4 cut(s) 505, 877, 1885, 2510
PsyI GACNNNGTC 1 cut(s) 278
RsaI GTAC 3 cut(s) 7, 2606, 3356
RsaNI GTAC 3 cut(s) 6, 2605, 3355
RseI CAYNNNNRTG 3 cut(s) 480, 2633, 2678
SatI GCNGC 6 cut(s) 264, 660, 2154, 3500, 3768, 3771
Sau96I GGNCC 9 cut(s) 180, 1015, 1282, 2498, 2780, 3403, 3649, 3746, 3793
SchI GAGTC 5 cut(s) 1642, 2201, 2746, 3559, 3769
ScrFI CCNGG 7 cut(s) 50, 178, 806, 1334, 2502, 3407, 3935
SduI GDGCHC 1 cut(s) 2074
SfaNI GCATC 7 cut(s) 9, 646, 714, 802, 1285, 3088, 3432
SfcI CTRYAG 3 cut(s) 394, 1566, 3023
SfoI GGCGCC 1 cut(s) 112
Sfr274I CTCGAG 1 cut(s) 1575
SgrAI CRCCGGYG 2 cut(s) 155, 215
SinI GGWCC 7 cut(s) 180, 1015, 1282, 3403, 3649, 3746, 3793
SlaI CTCGAG 1 cut(s) 1575
SmiMI CAYNNNNRTG 3 cut(s) 480, 2633, 2678
SmlI CTYRAG 9 cut(s) 140, 603, 1575, 1762, 1792, 2197, 2367, 2906, 3147
SmoI CTYRAG 9 cut(s) 140, 603, 1575, 1762, 1792, 2197, 2367, 2906, 3147
SphI GCATGC 1 cut(s) 3878
SseBI AGGCCT 3 cut(s) 1760, 1928, 2399
SsiI CCGC 5 cut(s) 250, 264, 799, 2638, 3755
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 2 cut(s) 3298, 3887
SspMI CTAG 9 cut(s) 777, 848, 1223, 1917, 2579, 3018, 3614, 3713, 3719
StuI AGGCCT 3 cut(s) 1760, 1928, 2399
StyD4I CCNGG 7 cut(s) 48, 176, 804, 1332, 2500, 3405, 3933
StyI CCWWGG 4 cut(s) 561, 1776, 2400, 2514
TaiI ACGT 4 cut(s) 66, 279, 2466, 2986
TaqI TCGA 8 cut(s) 45, 132, 443, 456, 949, 1576, 3165, 3455
TaqII GACCGA 2 cut(s) 2741, 2831
TauI GCSGC 1 cut(s) 266
TscAI CASTG 8 cut(s) 522, 748, 792, 907, 1570, 2653, 2855, 3444
TseFI GTSAC 1 cut(s) 3895
TseI GCWGC 5 cut(s) 659, 2153, 3499, 3767, 3770
Tsp45I GTSAC 1 cut(s) 3895
TspGWI ACGGA 1 cut(s) 3759
TspRI CASTG 8 cut(s) 522, 748, 792, 907, 1570, 2653, 2855, 3444
Tth111I GACNNNGTC 1 cut(s) 278
Van91I CCANNNNNTGG 1 cut(s) 754
Vha464I CTTAAG 1 cut(s) 2906
VpaK11BI GGWCC 7 cut(s) 180, 1015, 1282, 3403, 3649, 3746, 3793
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 8 cut(s) 765, 945, 966, 1249, 2804, 3030, 3182, 3192
XbaI TCTAGA 1 cut(s) 1222
XceI RCATGY 2 cut(s) 1462, 3878
XhoI CTCGAG 1 cut(s) 1575
XmiI GTMKAC 2 cut(s) 12, 557
XmnI GAANNNNTTC 1 cut(s) 2760
XspI CTAG 9 cut(s) 777, 848, 1223, 1917, 2579, 3018, 3614, 3713, 3719
ZraI GACGTC 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.