Rh4AG190400

Putative RNA methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001144
Physical Location & Seq
Reverse (-)
47146223 .. 47146821
599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG190400.1

Sequence Viewer

Length: 498 bp
ATGATTGATGACCCCAGCAACCATCCTCATCCTCCTCAGGCCTCCTCAACTAGATGCCCCCACCTCTCCGACCCCAAAGCCATCGCTGCCATGACCCACGCCGACTTCGACGTGGCCCAGACCCACTCCGTCCTCAACGCCCTCGACAACTGCCCCGACCATGAAACCGTCAACACTTCCAAGGCCAAGCTCGCCCACATCGAATCCACTCTCTCCAAGAAGCTCGAGGACCTCGTCCTTGCCTATATTGTTAACGGAGACTCACATAACAAAACCAATAAGAAGAAGAAGAACAAGAAATCCAAAACCGACACAGCCGCCGTAATTCCCACCTTCACCGTAGCCATCGCCGGTTCCATCATTCACAACGCTCAGTCCCTCGAACTCACCACTCGGATAGCTCGTGCCGTCACTATTTTCCGAGTTAATGAGGTGGTGGTATTTGACAACAACAGTGAGTCTGGCTCTCTAGAAGCTAATGATTTCGATGAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltrn_RNA_3

Protein Analysis

165

Amino Acids

17.94

Weight (kDa)

6.11

Isoelectric Point (pI)

33.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltrn_RNA_3 PF02598 111 - 157 4.9e-11 Putative RNA methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 318
AjiI CACGTC 1 cut(s) 112
AloI GAACNNNNNNTCC 2 cut(s) 284, 316
AluBI AGCT 4 cut(s) 190, 223, 401, 476
AluI AGCT 4 cut(s) 190, 223, 401, 476
Alw26I GTCTC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 3 cut(s) 39, 114, 183
ApeKI GCWGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 115, 229
AsuHPI GGTGA 2 cut(s) 328, 379
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 229
AxyI CCTNAGG 1 cut(s) 36
BauI CACGAG 1 cut(s) 402
BbvI GCAGC 1 cut(s) 73
BccI CCATC 4 cut(s) 30, 89, 353, 365
BceAI ACGGC 2 cut(s) 305, 392
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 2 cut(s) 51, 470
BisI GCNGC 2 cut(s) 87, 318
BlsI GCNGC 2 cut(s) 88, 319
Bme18I GGWCC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 224
BmgBI CACGTC 1 cut(s) 112
BmgT120I GGNCC 2 cut(s) 115, 229
BmiI GGNNCC 1 cut(s) 355
BmsI GCATC 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 449, 481
BsaJI CCNNGG 1 cut(s) 180
Bse118I RCCGGY 1 cut(s) 350
Bse21I CCTNAGG 1 cut(s) 36
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 22, 28
BseMII CTCAG 2 cut(s) 50, 386
BseRI GAGGAG 2 cut(s) 24, 34
BseXI GCAGC 1 cut(s) 73
BseYI CCCAGC 1 cut(s) 14
BshFI GGCC 3 cut(s) 41, 116, 185
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 1 cut(s) 351
BslFI GGGAC 1 cut(s) 361
BsmAI GTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 361
BsnI GGCC 3 cut(s) 41, 116, 185
BsoBI CYCGRG 1 cut(s) 224
BspACI CCGC 1 cut(s) 318
BspANI GGCC 3 cut(s) 41, 116, 185
BspCNI CTCAG 2 cut(s) 49, 385
BspLI GGNNCC 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 350
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 180
BssSI CACGAG 1 cut(s) 402
BssT1I CCWWGG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 169, 340, 455
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 2 cut(s) 36, 372
BstF5I GGATG 2 cut(s) 22, 28
BstMAI GTCTC 1 cut(s) 252
BstMWI GCNNNNNNNGC 2 cut(s) 86, 191
BstV1I GCAGC 1 cut(s) 73
Bsu36I CCTNAGG 1 cut(s) 36
BsuRI GGCC 3 cut(s) 41, 116, 185
BtgZI GCGATG 2 cut(s) 67, 331
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 22, 28
BtsIMutI CAGTG 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 2 cut(s) 115, 229
CviAII CATG 2 cut(s) 91, 161
DdeI CTNAG 2 cut(s) 36, 372
Eco130I CCWWGG 1 cut(s) 180
Eco147I AGGCCT 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 229
Eco81I CCTNAGG 1 cut(s) 36
Eco88I CYCGRG 1 cut(s) 224
EcoO109I RGGNCCY 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 2 cut(s) 94, 164
FaiI YATR 4 cut(s) 92, 162, 246, 267
FaqI GGGAC 1 cut(s) 361
FatI CATG 2 cut(s) 90, 160
Fnu4HI GCNGC 2 cut(s) 87, 318
FokI GGATG 2 cut(s) 9, 15
Fsp4HI GCNGC 2 cut(s) 87, 318
FspBI CTAG 2 cut(s) 51, 470
GluI GCNGC 2 cut(s) 87, 318
GsaI CCCAGC 1 cut(s) 18
HaeIII GGCC 3 cut(s) 41, 116, 185
HapII CCGG 1 cut(s) 351
Hin1II CATG 2 cut(s) 94, 164
HincII GTYRAC 2 cut(s) 172, 253
HindII GTYRAC 2 cut(s) 172, 253
HinfI GANTC 3 cut(s) 203, 260, 458
HpaI GTTAAC 1 cut(s) 253
HpaII CCGG 1 cut(s) 351
HphI GGTGA 2 cut(s) 328, 379
Hpy166II GTNNAC 2 cut(s) 172, 253
Hpy188I TCNGA 3 cut(s) 70, 396, 422
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 2 cut(s) 172, 253
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 3 cut(s) 169, 340, 455
HpyCH4IV ACGT 1 cut(s) 111
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 191
HpyF3I CTNAG 2 cut(s) 36, 372
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 2 cut(s) 94, 164
KspAI GTTAAC 1 cut(s) 253
LpnPI CCDG 5 cut(s) 23, 28, 131, 364, 447
Lsp1109I GCAGC 1 cut(s) 73
LweI GCATC 1 cut(s) 44
MaeI CTAG 2 cut(s) 51, 470
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 409
MboII GAAGA 3 cut(s) 295, 298, 301
MluCI AATT 2 cut(s) 324, 493
MlyI GAGTC 2 cut(s) 254, 467
MmeI TCCRAC 1 cut(s) 93
MseI TTAA 3 cut(s) 252, 426, 496
MspI CCGG 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 86, 191
NlaIII CATG 2 cut(s) 94, 164
NlaIV GGNNCC 1 cut(s) 355
NmuCI GTSAC 1 cut(s) 409
PaeR7I CTCGAG 1 cut(s) 224
PceI AGGCCT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 4 cut(s) 105, 198, 231, 400
PfeI GAWTC 1 cut(s) 203
PflFI GACNNNGTC 1 cut(s) 233
PkrI GCNGC 2 cut(s) 88, 319
PleI GAGTC 2 cut(s) 254, 466
PpsI GAGTC 2 cut(s) 254, 466
PpuMI RGGWCCY 1 cut(s) 229
Psp5II RGGWCCY 1 cut(s) 229
PspFI CCCAGC 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 355
PspPI GGNCC 2 cut(s) 115, 229
PspPPI RGGWCCY 1 cut(s) 229
PspXI VCTCGAGB 1 cut(s) 224
PsyI GACNNNGTC 1 cut(s) 233
SaqAI TTAA 3 cut(s) 252, 426, 496
SatI GCNGC 2 cut(s) 87, 318
Sau96I GGNCC 2 cut(s) 115, 229
SchI GAGTC 2 cut(s) 254, 467
SetI ASST 9 cut(s) 66, 114, 192, 225, 234, 335, 403, 435, 478
SfaNI GCATC 1 cut(s) 44
Sfr274I CTCGAG 1 cut(s) 224
SinI GGWCC 1 cut(s) 229
SlaI CTCGAG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 2 cut(s) 324, 493
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 1 cut(s) 318
SspMI CTAG 2 cut(s) 51, 470
StuI AGGCCT 1 cut(s) 41
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 3 cut(s) 169, 340, 455
TaiI ACGT 1 cut(s) 114
TaqI TCGA 6 cut(s) 108, 144, 201, 225, 381, 486
TasI AATT 2 cut(s) 324, 493
TauI GCSGC 1 cut(s) 320
TfiI GAWTC 1 cut(s) 203
Tru1I TTAA 3 cut(s) 252, 426, 496
Tru9I TTAA 3 cut(s) 252, 426, 496
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 2 cut(s) 118, 270
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 229
XbaI TCTAGA 1 cut(s) 469
XhoI CTCGAG 1 cut(s) 224
XspI CTAG 2 cut(s) 51, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.