Rw0G008450

Leucine-rich repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00321
OG0002555
Physical Location & Seq
Forward (+)
58178 .. 61399
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008450.1

Sequence Viewer

Length: 282 bp
ATGCAGCTACACGAAGTCCCATCATATACCTTGAGTTTACAGCAACTGCGGATCCTAGACTTCAGCCAAAATTCCTTGCAGTCGGTTCCAGTGGAGTTCAAAAATTTTACTTCACTCACTGAGCTAGGCCTATCTGACAACAACATATCAACACTTCCCCCAAAATTGGTCAGCATATTGCTACTGAAGTGTCAAGCTCTTCATATGAATTTGATCTTTGAGATGACTAAATCTCCTTGTCGGGGGTCAACGTGGCTGATCAGGGCTGTCAAAGACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box LRR_1 LRR_5 LRR_8

Protein Analysis

93

Amino Acids

10.54

Weight (kDa)

7.74

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 6 - 49 1.4e-07 Leucine rich repeat
LRR_4 PF12799 14 - 53 1.5e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 49
AclWI GGATC 2 cut(s) 46, 59
AcsI RAATTY 3 cut(s) 70, 103, 208
AcuI CTGAAG 2 cut(s) 46, 206
AfiI CCNNNNNNNGG 2 cut(s) 166, 242
AgsI TTSAA 1 cut(s) 100
AluBI AGCT 3 cut(s) 7, 124, 197
AluI AGCT 3 cut(s) 7, 124, 197
AlwI GGATC 2 cut(s) 46, 59
AlwNI CAGNNNCTG 1 cut(s) 46
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 3 cut(s) 70, 103, 208
BamHI GGATCC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 28
BclI TGATCA 1 cut(s) 258
BfaI CTAG 2 cut(s) 56, 125
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
BmiI GGNNCC 2 cut(s) 53, 87
BpuEI CTTGAG 1 cut(s) 52
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 242
Bse1I ACTGG 1 cut(s) 89
BseLI CCNNNNNNNGG 2 cut(s) 166, 242
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 1 cut(s) 89
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 129
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 2 cut(s) 166, 242
BsmFI GGGAC 1 cut(s) 2
BsnI GGCC 1 cut(s) 129
Bsp143I GATC 3 cut(s) 51, 213, 258
BspACI CCGC 1 cut(s) 49
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 53, 87
BspPI GGATC 2 cut(s) 46, 59
BspQI GCTCTTC 1 cut(s) 204
BsrI ACTGG 1 cut(s) 89
BssMI GATC 3 cut(s) 51, 213, 258
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 120
BstKTI GATC 3 cut(s) 54, 216, 261
BstMBI GATC 3 cut(s) 51, 213, 258
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BsuRI GGCC 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 96, 117
CaiI CAGNNNCTG 1 cut(s) 46
CviJI RGCY 7 cut(s) 7, 66, 124, 129, 197, 256, 266
CviKI_1 RGCY 7 cut(s) 7, 66, 124, 129, 197, 256, 266
DdeI CTNAG 1 cut(s) 120
DpnI GATC 3 cut(s) 53, 215, 260
DpnII GATC 3 cut(s) 51, 213, 258
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
Eco147I AGGCCT 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 46, 206
FaiI YATR 6 cut(s) 25, 27, 146, 176, 204, 206
FaqI GGGAC 1 cut(s) 2
FauNDI CATATG 1 cut(s) 204
FbaI TGATCA 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 5
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 2 cut(s) 56, 125
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 249
HindII GTYRAC 1 cut(s) 249
Hpy166II GTNNAC 2 cut(s) 38, 249
Hpy188I TCNGA 1 cut(s) 136
Hpy8I GTNNAC 2 cut(s) 38, 249
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 251
HpyCH4V TGCA 2 cut(s) 4, 79
HpyF3I CTNAG 1 cut(s) 120
HpySE526I ACGT 1 cut(s) 251
Ksp22I TGATCA 1 cut(s) 258
Kzo9I GATC 3 cut(s) 51, 213, 258
LguI GCTCTTC 1 cut(s) 204
LpnPI CCDG 2 cut(s) 102, 247
Lsp1109I GCAGC 1 cut(s) 16
MaeI CTAG 2 cut(s) 56, 125
MaeII ACGT 1 cut(s) 251
MalI GATC 3 cut(s) 53, 215, 260
MboI GATC 3 cut(s) 51, 213, 258
MboII GAAGA 1 cut(s) 191
MflI RGATCY 1 cut(s) 51
MluCI AATT 4 cut(s) 70, 103, 164, 208
NdeI CATATG 1 cut(s) 204
NdeII GATC 3 cut(s) 51, 213, 258
NlaIV GGNNCC 2 cut(s) 53, 87
PceI AGGCCT 1 cut(s) 129
PciSI GCTCTTC 1 cut(s) 204
PkrI GCNGC 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 53, 87
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 204
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 51, 213, 258
SetI ASST 5 cut(s) 9, 32, 126, 199, 254
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 4 cut(s) 70, 103, 164, 208
SseBI AGGCCT 1 cut(s) 129
SsiI CCGC 1 cut(s) 49
SspMI CTAG 2 cut(s) 56, 125
StuI AGGCCT 1 cut(s) 129
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 1 cut(s) 254
TasI AATT 4 cut(s) 70, 103, 164, 208
TscAI CASTG 2 cut(s) 96, 124
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 191, 221
TspRI CASTG 2 cut(s) 96, 124
XapI RAATTY 3 cut(s) 70, 103, 208
XspI CTAG 2 cut(s) 56, 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.