Rw4G030630

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002555
Physical Location & Seq
Forward (+)
57360341 .. 57361944
1604 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030630.1

Sequence Viewer

Length: 741 bp
ATGGCGAATTATTGGAGATATTTCCGGCTGGATGGGGATCCACCTGTGTTTTCAGGAGAGGTGCCAAAGGATGTGGATGATGAAAAGGAGCCAGTGGATGTAGATCCACTACATGTAGATGATGATGAAGAGGTTCCTCTGGATGGAATTATGCCTGAAAACTGGGAATATACTGATGATGGGCCTGAAGTGCAAAGCAATGGTCCAGAAATGAGTGAAGCTGCTAATGGGCCTGAAGTGCAGAGTGGTGCTCAAGCCCGTGGCCCAACTATTACAACTGCAAGAGGCAGAGATCATCAAGCATCCACTTCAAGACAAAATGATAAAGGGAAGCAGAAAGTTATAGAGGAAGAAGTTTGTGTTCCAAAGAAAAAGCATGGTAGGCCGAAACAGAGGGGCACTAGGAGGTATGATACGAGGTCTGGTGGTGAATGCTCAAACCCGGTTCAGTATGATTGGAGCAGTACTAGTAGTGATGATTCTGATGATCCAAACTGGGATGGGATTAGGGATGGTGACTTCGAGTTGGTTTCTGAAGATGATGAAGTAGACTTTGAGGCACATGTGGATGGTGATCCCGAACATGTAGAGGAATGGAATGACATTGGATTCAGAGGCAATATATCTGATGATGATGGTACTGCTAGTGATGGCTTTTCAAGCCTGCATGGTTCAAGTGATGAGTCTGGAGAAGATGGGGGGAGGTATCCAGTGAAACACAAGAGGATGTTCTCGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00592 ko01110 map00592 map01110
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

246

Amino Acids

27.42

Weight (kDa)

4.33

Isoelectric Point (pI)

44.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 549
AclWI GGATC 5 cut(s) 32, 45, 98, 482, 569
AcuI CTGAAG 3 cut(s) 207, 255, 555
AfaI GTAC 2 cut(s) 466, 640
AfiI CCNNNNNNNGG 1 cut(s) 143
AflIII ACRYGT 3 cut(s) 112, 562, 583
AgsI TTSAA 3 cut(s) 312, 660, 675
AhlI ACTAGT 1 cut(s) 467
AluBI AGCT 1 cut(s) 221
AluI AGCT 1 cut(s) 221
Alw21I GWGCWC 1 cut(s) 253
AlwI GGATC 5 cut(s) 32, 45, 98, 482, 569
Ama87I CYCGRG 1 cut(s) 733
AoxI GGCC 4 cut(s) 182, 230, 262, 383
ApeKI GCWGC 1 cut(s) 221
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
Asp700I GAANNNNTTC 1 cut(s) 132
AspS9I GGNCC 4 cut(s) 182, 203, 230, 263
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 3 cut(s) 440, 527, 584
AvaI CYCGRG 1 cut(s) 733
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 401
BamHI GGATCC 1 cut(s) 37
BanI GGYRCC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 253
BbvI GCAGC 1 cut(s) 208
BccI CCATC 9 cut(s) 26, 137, 173, 494, 506, 563, 629, 644, 689
BciVI GTATCC 1 cut(s) 717
BcnI CCSGG 1 cut(s) 443
BcuI ACTAGT 1 cut(s) 467
BfaI CTAG 3 cut(s) 402, 468, 645
BfuI GTATCC 1 cut(s) 717
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
BmcAI AGTACT 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 443
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 733
BmgT120I GGNCC 4 cut(s) 182, 203, 230, 263
BmiI GGNNCC 4 cut(s) 39, 63, 90, 135
BmrFI CCNGG 1 cut(s) 443
BmrI ACTGGG 2 cut(s) 172, 505
BmsI GCATC 1 cut(s) 311
BmuI ACTGGG 2 cut(s) 172, 505
BplI GAGNNNNNCTC 2 cut(s) 235, 267
BpmI CTGGAG 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 237
BpuMI CCSGG 1 cut(s) 443
BsaBI GATNNNNATC 3 cut(s) 36, 102, 573
BsaJI CCNNGG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 143
Bse1I ACTGG 4 cut(s) 92, 167, 500, 710
Bse3DI GCAATG 1 cut(s) 205
Bse8I GATNNNNATC 3 cut(s) 36, 102, 573
BseDI CCNNGG 1 cut(s) 259
BseJI GATNNNNATC 3 cut(s) 36, 102, 573
BseLI CCNNNNNNNGG 1 cut(s) 143
BseMI GCAATG 1 cut(s) 205
BseNI ACTGG 4 cut(s) 92, 167, 500, 710
BseSI GKGCMC 1 cut(s) 401
BseXI GCAGC 1 cut(s) 208
BsgI GTGCAG 1 cut(s) 260
BshFI GGCC 4 cut(s) 184, 232, 264, 385
BshNI GGYRCC 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 733
BsiSI CCGG 2 cut(s) 25, 443
BslI CCNNNNNNNGG 1 cut(s) 143
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 4 cut(s) 184, 232, 264, 385
BsoBI CYCGRG 1 cut(s) 733
Bsp1286I GDGCHC 2 cut(s) 253, 401
Bsp143I GATC 5 cut(s) 37, 103, 292, 487, 574
BspANI GGCC 4 cut(s) 184, 232, 264, 385
BspLI GGNNCC 4 cut(s) 39, 63, 90, 135
BspPI GGATC 5 cut(s) 32, 45, 98, 482, 569
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 205
BsrI ACTGG 4 cut(s) 92, 167, 500, 710
BssECI CCNNGG 1 cut(s) 259
BssMI GATC 5 cut(s) 37, 103, 292, 487, 574
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 665
BstDSI CCRYGG 1 cut(s) 259
BstKTI GATC 5 cut(s) 40, 106, 295, 490, 577
BstMBI GATC 5 cut(s) 37, 103, 292, 487, 574
BstMWI GCNNNNNNNGC 4 cut(s) 190, 238, 382, 660
BstNSI RCATGY 3 cut(s) 116, 566, 587
BstSCI CCNGG 1 cut(s) 441
BstSLI GKGCMC 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 208
BstX2I RGATCY 2 cut(s) 37, 103
BstYI RGATCY 2 cut(s) 37, 103
BsuI GTATCC 1 cut(s) 717
BsuRI GGCC 4 cut(s) 184, 232, 264, 385
BtgI CCRYGG 1 cut(s) 259
BtsIMutI CAGTG 2 cut(s) 99, 717
Cac8I GCNNGC 1 cut(s) 665
Cfr13I GGNCC 4 cut(s) 182, 203, 230, 263
Csp6I GTAC 2 cut(s) 465, 639
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 5 cut(s) 113, 377, 563, 584, 668
CviQI GTAC 2 cut(s) 465, 639
DpnI GATC 5 cut(s) 39, 105, 294, 489, 576
DpnII GATC 5 cut(s) 37, 103, 292, 487, 574
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 3 cut(s) 207, 255, 555
Eco88I CYCGRG 1 cut(s) 733
FaeI CATG 5 cut(s) 116, 380, 566, 587, 671
FatI CATG 5 cut(s) 112, 376, 562, 583, 667
FblI GTMKAC 1 cut(s) 549
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 9 cut(s) 44, 83, 89, 110, 155, 289, 512, 524, 581
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 3 cut(s) 402, 468, 645
GluI GCNGC 1 cut(s) 222
GsuI CTGGAG 1 cut(s) 708
HaeIII GGCC 4 cut(s) 184, 232, 264, 385
HapII CCGG 2 cut(s) 25, 443
Hin1II CATG 5 cut(s) 116, 380, 566, 587, 671
HinfI GANTC 3 cut(s) 479, 609, 683
HpaII CCGG 2 cut(s) 25, 443
HphI GGTGA 3 cut(s) 440, 527, 584
Hpy166II GTNNAC 1 cut(s) 550
Hpy188I TCNGA 4 cut(s) 484, 535, 614, 628
Hpy188III TCNNGA 7 cut(s) 54, 140, 206, 312, 578, 687, 733
Hpy8I GTNNAC 1 cut(s) 550
HpyCH4V TGCA 4 cut(s) 193, 241, 281, 667
HpyF10VI GCNNNNNNNGC 4 cut(s) 190, 238, 382, 660
Hsp92II CATG 5 cut(s) 116, 380, 566, 587, 671
Kzo9I GATC 5 cut(s) 37, 103, 292, 487, 574
LmnI GCTCC 2 cut(s) 88, 459
Lsp1109I GCAGC 1 cut(s) 208
LweI GCATC 1 cut(s) 311
MaeI CTAG 3 cut(s) 402, 468, 645
MaeIII GTNAC 1 cut(s) 515
MalI GATC 5 cut(s) 39, 105, 294, 489, 576
MboI GATC 5 cut(s) 37, 103, 292, 487, 574
MboII GAAGA 4 cut(s) 140, 362, 548, 704
MflI RGATCY 2 cut(s) 37, 103
MhlI GDGCHC 2 cut(s) 253, 401
MluCI AATT 2 cut(s) 7, 147
MlyI GAGTC 1 cut(s) 692
MroXI GAANNNNTTC 1 cut(s) 132
MslI CAYNNNNRTG 2 cut(s) 117, 567
MspI CCGG 2 cut(s) 25, 443
MspR9I CCNGG 1 cut(s) 443
Mva1269I GAATGC 1 cut(s) 437
MwoI GCNNNNNNNGC 4 cut(s) 190, 238, 382, 660
NciI CCSGG 1 cut(s) 443
NdeII GATC 5 cut(s) 37, 103, 292, 487, 574
NlaIII CATG 5 cut(s) 116, 380, 566, 587, 671
NlaIV GGNNCC 4 cut(s) 39, 63, 90, 135
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 3 cut(s) 116, 566, 587
PaeR7I CTCGAG 1 cut(s) 733
PciI ACATGT 3 cut(s) 112, 562, 583
PctI GAATGC 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 132
PfeI GAWTC 2 cut(s) 479, 609
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 1 cut(s) 691
PpsI GAGTC 1 cut(s) 691
PscI ACATGT 3 cut(s) 112, 562, 583
PspN4I GGNNCC 4 cut(s) 39, 63, 90, 135
PspPI GGNCC 4 cut(s) 182, 203, 230, 263
PsuI RGATCY 2 cut(s) 37, 103
RsaI GTAC 2 cut(s) 466, 640
RsaNI GTAC 2 cut(s) 465, 639
RseI CAYNNNNRTG 2 cut(s) 117, 567
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 5 cut(s) 37, 103, 292, 487, 574
Sau96I GGNCC 4 cut(s) 182, 203, 230, 263
ScaI AGTACT 1 cut(s) 466
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 1 cut(s) 443
SduI GDGCHC 2 cut(s) 253, 401
SetI ASST 8 cut(s) 46, 63, 135, 223, 410, 422, 707, 740
SfaNI GCATC 1 cut(s) 311
Sfr274I CTCGAG 1 cut(s) 733
SinI GGWCC 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 733
SmiMI CAYNNNNRTG 2 cut(s) 117, 567
SmlI CTYRAG 2 cut(s) 252, 733
SmoI CTYRAG 2 cut(s) 252, 733
SpeI ACTAGT 1 cut(s) 467
Sse9I AATT 2 cut(s) 7, 147
SspMI CTAG 3 cut(s) 402, 468, 645
StyD4I CCNGG 1 cut(s) 441
TaqI TCGA 2 cut(s) 522, 734
TasI AATT 2 cut(s) 7, 147
TatI WGTACW 1 cut(s) 464
TfiI GAWTC 2 cut(s) 479, 609
TscAI CASTG 2 cut(s) 99, 717
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 96, 141, 558
TspRI CASTG 2 cut(s) 99, 717
VpaK11BI GGWCC 1 cut(s) 203
XceI RCATGY 3 cut(s) 116, 566, 587
XhoI CTCGAG 1 cut(s) 733
XmiI GTMKAC 1 cut(s) 549
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 3 cut(s) 402, 468, 645
ZrmI AGTACT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.