Type III restriction enzyme, res subunit
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G36020 |
| fragaria_vesca | FvH4_7g03750 |
| malus_domestica | MD15G1404400.v1.1 |
| prunus_persica | Prupe.1G551100_v2.0.a1 Prupe.2G019600_v2.0.a1 Prupe.2G019700_v2.0.a1 |
| pyrus_communis | pycom15g36170 pycom15g36180 |
| rosa_chinensis | RchiOBHm_Chr7g0228871 RchiOBHm_Chr7g0228881 RchiOBHm_Chr7g0228951 |
| rosa_laevigata | RLG00000001539 RLG00000002150 RLG00000022432 |
| rosa_multiflora | Rmu_sc0000721.1_g000006 Rmu_sc0004517.1_g000001 Rmu_sc0005913.1_g000001 Rmu_sc0005913.1_g000026 Rmu_sc0011218.1_g000006 Rmu_sc0013945.1_g000010 |
| rosa_roxburghii | Rroxscaffold_3G00230830 Rroxscaffold_3G00238230 |
| rosa_rugosa | Rorug07G0252600 Rorug07G0254200 |
| rosa_samantha | Rh7AG405800 Rh7BG333000 Rh7BG385100 Rh7BG385200 Rh7BG385300 Rh7CG423400 Rh7DG341000 Rh7DG399800 Rh7DG399900 |
| rosa_wichuraiana | Rw7G033620 Rw7G033650 Rw7G033670 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1740, 2814 |
| AarI | CACCTGC | 3 cut(s) | 184, 1861, 3677 |
| Acc36I | ACCTGC | 3 cut(s) | 184, 1861, 3677 |
| Acc65I | GGTACC | 1 cut(s) | 775 |
| AccB1I | GGYRCC | 3 cut(s) | 493, 775, 2492 |
| AccBSI | CCGCTC | 1 cut(s) | 2655 |
| AccI | GTMKAC | 2 cut(s) | 2825, 3034 |
| AccII | CGCG | 1 cut(s) | 3114 |
| AciI | CCGC | 7 cut(s) | 437, 491, 773, 2655, 2971, 3114, 3390 |
| AclWI | GGATC | 6 cut(s) | 13, 293, 826, 1385, 2114, 3783 |
| AcoI | YGGCCR | 1 cut(s) | 2868 |
| AcsI | RAATTY | 9 cut(s) | 44, 203, 1423, 1542, 1565, 1725, 2566, 2717, 3575 |
| AcuI | CTGAAG | 1 cut(s) | 442 |
| AcvI | CACGTG | 1 cut(s) | 1790 |
| AcyI | GRCGYC | 1 cut(s) | 494 |
| AfaI | GTAC | 4 cut(s) | 777, 1074, 3546, 3701 |
| AfeI | AGCGCT | 1 cut(s) | 2956 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 290, 1112, 1897, 2149, 3833 |
| AflIII | ACRYGT | 2 cut(s) | 587, 677 |
| AjiI | CACGTC | 1 cut(s) | 1979 |
| AjnI | CCWGG | 6 cut(s) | 328, 814, 837, 1111, 3073, 3832 |
| AloI | GAACNNNNNNTCC | 1 cut(s) | 29 |
| Alw21I | GWGCWC | 3 cut(s) | 1333, 1962, 2071 |
| Alw26I | GTCTC | 9 cut(s) | 178, 1437, 1817, 1853, 2204, 2495, 2504, 3019, 3199 |
| Alw44I | GTGCAC | 1 cut(s) | 1958 |
| AlwI | GGATC | 6 cut(s) | 13, 293, 826, 1385, 2114, 3783 |
| Ama87I | CYCGRG | 1 cut(s) | 3049 |
| Aor51HI | AGCGCT | 1 cut(s) | 2956 |
| AoxI | GGCC | 6 cut(s) | 503, 831, 1114, 1250, 1528, 2868 |
| ApaLI | GTGCAC | 1 cut(s) | 1958 |
| ApeKI | GCWGC | 9 cut(s) | 59, 308, 434, 517, 1157, 1309, 2008, 2952, 3632 |
| ApoI | RAATTY | 9 cut(s) | 44, 203, 1423, 1542, 1565, 1725, 2566, 2717, 3575 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 2300, 3000, 3535 |
| Asp718I | GGTACC | 1 cut(s) | 775 |
| AspLEI | GCGC | 2 cut(s) | 496, 2957 |
| AspS9I | GGNCC | 2 cut(s) | 1250, 3419 |
| AsuHPI | GGTGA | 9 cut(s) | 718, 1622, 2090, 2232, 2561, 2664, 2727, 2986, 3351 |
| AsuII | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| AvaI | CYCGRG | 1 cut(s) | 3049 |
| AvaII | GGWCC | 1 cut(s) | 3419 |
| AxyI | CCTNAGG | 2 cut(s) | 303, 2907 |
| BaeGI | GKGCMC | 1 cut(s) | 1962 |
| BalI | TGGCCA | 1 cut(s) | 2870 |
| BanI | GGYRCC | 3 cut(s) | 493, 775, 2492 |
| BanII | GRGCYC | 1 cut(s) | 2071 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 440, 472 |
| BbrPI | CACGTG | 1 cut(s) | 1790 |
| BbsI | GAAGAC | 8 cut(s) | 42, 377, 1403, 2293, 2763, 3163, 3462, 3816 |
| Bbv12I | GWGCWC | 3 cut(s) | 1333, 1962, 2071 |
| BbvCI | CCTCAGC | 2 cut(s) | 2724, 3189 |
| BbvI | GCAGC | 9 cut(s) | 71, 320, 421, 529, 1169, 1321, 2020, 2964, 3644 |
| BccI | CCATC | 9 cut(s) | 35, 456, 763, 842, 1687, 1957, 3319, 3845, 3880 |
| BceAI | ACGGC | 3 cut(s) | 55, 171, 2648 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 3195, 3229 |
| BciT130I | CCWGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| BclI | TGATCA | 2 cut(s) | 1372, 3147 |
| BcoDI | GTCTC | 9 cut(s) | 178, 1437, 1817, 1853, 2204, 2495, 2504, 3019, 3199 |
| BfaI | CTAG | 8 cut(s) | 9, 501, 1197, 1229, 1260, 1643, 2507, 3737 |
| BfmI | CTRYAG | 2 cut(s) | 1854, 3285 |
| BfoI | RGCGCY | 2 cut(s) | 497, 2958 |
| BfuAI | ACCTGC | 3 cut(s) | 184, 1861, 3677 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 502, 3669 |
| BglII | AGATCT | 3 cut(s) | 2128, 2710, 3101 |
| BmcAI | AGTACT | 1 cut(s) | 1074 |
| Bme1390I | CCNGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| Bme18I | GGWCC | 1 cut(s) | 3419 |
| BmeT110I | CYCGRG | 1 cut(s) | 3049 |
| BmgBI | CACGTC | 1 cut(s) | 1979 |
| BmgT120I | GGNCC | 2 cut(s) | 1250, 3419 |
| BmiI | GGNNCC | 6 cut(s) | 15, 495, 777, 945, 2494, 3421 |
| BmrFI | CCNGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| BpiI | GAAGAC | 8 cut(s) | 42, 377, 1403, 2293, 2763, 3163, 3462, 3816 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2833, 2865 |
| BpmI | CTGGAG | 3 cut(s) | 1812, 2363, 3003 |
| Bpu10I | CCTNAGC | 2 cut(s) | 2724, 3189 |
| Bpu14I | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| BpuEI | CTTGAG | 1 cut(s) | 3282 |
| BsaAI | YACGTR | 2 cut(s) | 1790, 3032 |
| BsaHI | GRCGYC | 1 cut(s) | 494 |
| BsaI | GGTCTC | 1 cut(s) | 3199 |
| BsaJI | CCNNGG | 7 cut(s) | 838, 948, 1953, 2630, 2686, 2925, 3648 |
| BsaWI | WCCGGW | 1 cut(s) | 229 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 796, 826, 1464, 1494, 1810, 1840, 2568, 2598 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 290, 1112, 1897, 2149, 3833 |
| Bse118I | RCCGGY | 1 cut(s) | 3367 |
| Bse1I | ACTGG | 8 cut(s) | 801, 1371, 1531, 1708, 2312, 2420, 3244, 3355 |
| Bse21I | CCTNAGG | 2 cut(s) | 303, 2907 |
| Bse3DI | GCAATG | 4 cut(s) | 1943, 3525, 3688, 3756 |
| BseBI | CCWGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| BseDI | CCNNGG | 7 cut(s) | 838, 948, 1953, 2630, 2686, 2925, 3648 |
| BseGI | GGATG | 9 cut(s) | 119, 834, 1296, 1686, 1698, 1949, 2276, 2602, 3160 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 290, 1112, 1897, 2149, 3833 |
| BseMI | GCAATG | 4 cut(s) | 1943, 3525, 3688, 3756 |
| BseNI | ACTGG | 8 cut(s) | 801, 1371, 1531, 1708, 2312, 2420, 3244, 3355 |
| BseRI | GAGGAG | 3 cut(s) | 1662, 1823, 2838 |
| BseSI | GKGCMC | 1 cut(s) | 1962 |
| BseXI | GCAGC | 9 cut(s) | 71, 320, 421, 529, 1169, 1321, 2020, 2964, 3644 |
| BseYI | CCCAGC | 1 cut(s) | 1574 |
| Bsh1236I | CGCG | 1 cut(s) | 3114 |
| BshFI | GGCC | 6 cut(s) | 505, 833, 1116, 1252, 1530, 2870 |
| BshNI | GGYRCC | 3 cut(s) | 493, 775, 2492 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1333, 1962, 2071 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3049 |
| BsiSI | CCGG | 4 cut(s) | 230, 2392, 3368, 3565 |
| BslFI | GGGAC | 1 cut(s) | 426 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 290, 1112, 1897, 2149, 3833 |
| BsmAI | GTCTC | 9 cut(s) | 178, 1437, 1817, 1853, 2204, 2495, 2504, 3019, 3199 |
| BsmBI | CGTCTC | 1 cut(s) | 1853 |
| BsmFI | GGGAC | 1 cut(s) | 426 |
| BsmI | GAATGC | 4 cut(s) | 1972, 2888, 3604, 3722 |
| BsnI | GGCC | 6 cut(s) | 505, 833, 1116, 1252, 1530, 2870 |
| Bso31I | GGTCTC | 1 cut(s) | 3199 |
| BsoBI | CYCGRG | 1 cut(s) | 3049 |
| Bsp119I | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| Bsp1286I | GDGCHC | 3 cut(s) | 1333, 1962, 2071 |
| BspACI | CCGC | 7 cut(s) | 437, 491, 773, 2655, 2971, 3114, 3390 |
| BspANI | GGCC | 6 cut(s) | 505, 833, 1116, 1252, 1530, 2870 |
| BspFNI | CGCG | 1 cut(s) | 3114 |
| BspLI | GGNNCC | 6 cut(s) | 15, 495, 777, 945, 2494, 3421 |
| BspMAI | CTGCAG | 1 cut(s) | 1858 |
| BspMI | ACCTGC | 3 cut(s) | 184, 1861, 3677 |
| BspPI | GGATC | 6 cut(s) | 13, 293, 826, 1385, 2114, 3783 |
| BspQI | GCTCTTC | 1 cut(s) | 2082 |
| BspT104I | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| BspT107I | GGYRCC | 3 cut(s) | 493, 775, 2492 |
| BspTNI | GGTCTC | 1 cut(s) | 3199 |
| BsrBI | CCGCTC | 1 cut(s) | 2655 |
| BsrDI | GCAATG | 4 cut(s) | 1943, 3525, 3688, 3756 |
| BsrFI | RCCGGY | 1 cut(s) | 3367 |
| BsrI | ACTGG | 8 cut(s) | 801, 1371, 1531, 1708, 2312, 2420, 3244, 3355 |
| BssAI | RCCGGY | 1 cut(s) | 3367 |
| BssECI | CCNNGG | 7 cut(s) | 838, 948, 1953, 2630, 2686, 2925, 3648 |
| BssNI | GRCGYC | 1 cut(s) | 494 |
| BssT1I | CCWWGG | 4 cut(s) | 948, 1953, 2686, 2925 |
| Bst2UI | CCWGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| Bst4CI | ACNGT | 5 cut(s) | 669, 2252, 2492, 2609, 3820 |
| Bst6I | CTCTTC | 8 cut(s) | 176, 1227, 2082, 3006, 3017, 3202, 3351, 3480 |
| BstACI | GRCGYC | 1 cut(s) | 494 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 2014, 3638, 3728 |
| BstBAI | YACGTR | 2 cut(s) | 1790, 3032 |
| BstBI | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| BstC8I | GCNNGC | 5 cut(s) | 1551, 1872, 2497, 2959, 3839 |
| BstDSI | CCRYGG | 2 cut(s) | 2630, 3648 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1110, 3831 |
| BstF5I | GGATG | 9 cut(s) | 119, 834, 1296, 1686, 1698, 1949, 2276, 2602, 3160 |
| BstFNI | CGCG | 1 cut(s) | 3114 |
| BstH2I | RGCGCY | 2 cut(s) | 497, 2958 |
| BstHHI | GCGC | 2 cut(s) | 496, 2957 |
| BstMAI | GTCTC | 9 cut(s) | 178, 1437, 1817, 1853, 2204, 2495, 2504, 3019, 3199 |
| BstNI | CCWGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| BstNSI | RCATGY | 5 cut(s) | 591, 681, 1760, 1993, 3841 |
| BstSCI | CCNGG | 6 cut(s) | 328, 814, 837, 1111, 3073, 3832 |
| BstSFI | CTRYAG | 2 cut(s) | 1854, 3285 |
| BstSLI | GKGCMC | 1 cut(s) | 1962 |
| BstSNI | TACGTA | 1 cut(s) | 3032 |
| BstUI | CGCG | 1 cut(s) | 3114 |
| BstV1I | GCAGC | 9 cut(s) | 71, 320, 421, 529, 1169, 1321, 2020, 2964, 3644 |
| BstV2I | GAAGAC | 8 cut(s) | 42, 377, 1403, 2293, 2763, 3163, 3462, 3816 |
| BstX2I | RGATCY | 6 cut(s) | 5, 1390, 2128, 2710, 3101, 3788 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 561 |
| BstYI | RGATCY | 6 cut(s) | 5, 1390, 2128, 2710, 3101, 3788 |
| Bsu36I | CCTNAGG | 2 cut(s) | 303, 2907 |
| BsuRI | GGCC | 6 cut(s) | 505, 833, 1116, 1252, 1530, 2870 |
| BtgI | CCRYGG | 2 cut(s) | 2630, 3648 |
| BtrI | CACGTC | 1 cut(s) | 1979 |
| BtsCI | GGATG | 9 cut(s) | 119, 834, 1296, 1686, 1698, 1949, 2276, 2602, 3160 |
| BtsIMutI | CAGTG | 3 cut(s) | 1818, 2055, 2832 |
| BveI | ACCTGC | 3 cut(s) | 184, 1861, 3677 |
| Cac8I | GCNNGC | 5 cut(s) | 1551, 1872, 2497, 2959, 3839 |
| CfoI | GCGC | 2 cut(s) | 496, 2957 |
| Cfr10I | RCCGGY | 1 cut(s) | 3367 |
| Cfr13I | GGNCC | 2 cut(s) | 1250, 3419 |
| CseI | GACGC | 3 cut(s) | 1870, 2009, 3821 |
| Csp6I | GTAC | 4 cut(s) | 776, 1073, 3545, 3700 |
| CviQI | GTAC | 4 cut(s) | 776, 1073, 3545, 3700 |
| DinI | GGCGCC | 1 cut(s) | 495 |
| EaeI | YGGCCR | 1 cut(s) | 2868 |
| Eam1104I | CTCTTC | 8 cut(s) | 176, 1227, 2082, 3006, 3017, 3202, 3351, 3480 |
| EarI | CTCTTC | 8 cut(s) | 176, 1227, 2082, 3006, 3017, 3202, 3351, 3480 |
| Ecl136II | GAGCTC | 1 cut(s) | 2069 |
| Eco105I | TACGTA | 1 cut(s) | 3032 |
| Eco130I | CCWWGG | 4 cut(s) | 948, 1953, 2686, 2925 |
| Eco147I | AGGCCT | 2 cut(s) | 505, 1116 |
| Eco24I | GRGCYC | 1 cut(s) | 2071 |
| Eco31I | GGTCTC | 1 cut(s) | 3199 |
| Eco32I | GATATC | 2 cut(s) | 864, 1627 |
| Eco47I | GGWCC | 1 cut(s) | 3419 |
| Eco47III | AGCGCT | 1 cut(s) | 2956 |
| Eco53kI | GAGCTC | 1 cut(s) | 2069 |
| Eco57I | CTGAAG | 1 cut(s) | 442 |
| Eco72I | CACGTG | 1 cut(s) | 1790 |
| Eco81I | CCTNAGG | 2 cut(s) | 303, 2907 |
| Eco88I | CYCGRG | 1 cut(s) | 3049 |
| EcoICRI | GAGCTC | 1 cut(s) | 2069 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1110, 3831 |
| EcoRI | GAATTC | 3 cut(s) | 44, 1725, 2566 |
| EcoRII | CCWGG | 6 cut(s) | 328, 814, 837, 1111, 3073, 3832 |
| EcoRV | GATATC | 2 cut(s) | 864, 1627 |
| EcoT14I | CCWWGG | 4 cut(s) | 948, 1953, 2686, 2925 |
| EcoT22I | ATGCAT | 2 cut(s) | 1974, 3724 |
| EcoT38I | GRGCYC | 1 cut(s) | 2071 |
| EgeI | GGCGCC | 1 cut(s) | 495 |
| EheI | GGCGCC | 1 cut(s) | 495 |
| ErhI | CCWWGG | 4 cut(s) | 948, 1953, 2686, 2925 |
| Esp3I | CGTCTC | 1 cut(s) | 1853 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2697, 2729 |
| FaqI | GGGAC | 1 cut(s) | 426 |
| FauI | CCCGC | 1 cut(s) | 2964 |
| FauNDI | CATATG | 2 cut(s) | 1003, 1866 |
| FbaI | TGATCA | 2 cut(s) | 1372, 3147 |
| FblI | GTMKAC | 2 cut(s) | 2825, 3034 |
| FokI | GGATG | 9 cut(s) | 106, 821, 1303, 1693, 1705, 1936, 2263, 2609, 3167 |
| FriOI | GRGCYC | 1 cut(s) | 2071 |
| FspBI | CTAG | 8 cut(s) | 9, 501, 1197, 1229, 1260, 1643, 2507, 3737 |
| GlaI | GCGC | 2 cut(s) | 495, 2956 |
| GsaI | CCCAGC | 1 cut(s) | 1578 |
| GsuI | CTGGAG | 3 cut(s) | 1812, 2363, 3003 |
| HaeII | RGCGCY | 2 cut(s) | 497, 2958 |
| HaeIII | GGCC | 6 cut(s) | 505, 833, 1116, 1252, 1530, 2870 |
| HapII | CCGG | 4 cut(s) | 230, 2392, 3368, 3565 |
| HgaI | GACGC | 3 cut(s) | 1870, 2009, 3821 |
| HhaI | GCGC | 2 cut(s) | 496, 2957 |
| Hin1I | GRCGYC | 1 cut(s) | 494 |
| Hin6I | GCGC | 2 cut(s) | 494, 2955 |
| HinP1I | GCGC | 2 cut(s) | 494, 2955 |
| HincII | GTYRAC | 4 cut(s) | 265, 1639, 2158, 3319 |
| HindII | GTYRAC | 4 cut(s) | 265, 1639, 2158, 3319 |
| HindIII | AAGCTT | 3 cut(s) | 1298, 1844, 2047 |
| HpaI | GTTAAC | 1 cut(s) | 265 |
| HpaII | CCGG | 4 cut(s) | 230, 2392, 3368, 3565 |
| HphI | GGTGA | 9 cut(s) | 718, 1622, 2090, 2232, 2561, 2664, 2727, 2986, 3351 |
| Hpy99I | CGWCG | 1 cut(s) | 716 |
| HpyCH4III | ACNGT | 5 cut(s) | 669, 2252, 2492, 2609, 3820 |
| HpyCH4IV | ACGT | 5 cut(s) | 997, 1789, 1978, 2298, 3031 |
| HpySE526I | ACGT | 5 cut(s) | 997, 1789, 1978, 2298, 3031 |
| Hsp92I | GRCGYC | 1 cut(s) | 494 |
| HspAI | GCGC | 2 cut(s) | 494, 2955 |
| KasI | GGCGCC | 1 cut(s) | 493 |
| KpnI | GGTACC | 1 cut(s) | 779 |
| Ksp22I | TGATCA | 2 cut(s) | 1372, 3147 |
| KspAI | GTTAAC | 1 cut(s) | 265 |
| LguI | GCTCTTC | 1 cut(s) | 2082 |
| LmnI | GCTCC | 5 cut(s) | 1194, 1328, 2074, 2620, 3203 |
| Lsp1109I | GCAGC | 9 cut(s) | 71, 320, 421, 529, 1169, 1321, 2020, 2964, 3644 |
| MaeI | CTAG | 8 cut(s) | 9, 501, 1197, 1229, 1260, 1643, 2507, 3737 |
| MaeII | ACGT | 5 cut(s) | 997, 1789, 1978, 2298, 3031 |
| MaeIII | GTNAC | 8 cut(s) | 637, 890, 1320, 2110, 2670, 2750, 3235, 3357 |
| MbiI | CCGCTC | 1 cut(s) | 2655 |
| MflI | RGATCY | 6 cut(s) | 5, 1390, 2128, 2710, 3101, 3788 |
| MhlI | GDGCHC | 3 cut(s) | 1333, 1962, 2071 |
| MlsI | TGGCCA | 1 cut(s) | 2870 |
| MluNI | TGGCCA | 1 cut(s) | 2870 |
| Mly113I | GGCGCC | 1 cut(s) | 494 |
| MlyI | GAGTC | 9 cut(s) | 1819, 2354, 2506, 2678, 2777, 3314, 3412, 3446, 3512 |
| MmeI | TCCRAC | 2 cut(s) | 526, 2554 |
| Mox20I | TGGCCA | 1 cut(s) | 2870 |
| Mph1103I | ATGCAT | 2 cut(s) | 1974, 3724 |
| MroXI | GAANNNNTTC | 3 cut(s) | 2300, 3000, 3535 |
| MscI | TGGCCA | 1 cut(s) | 2870 |
| MslI | CAYNNNNRTG | 2 cut(s) | 559, 1137 |
| Msp20I | TGGCCA | 1 cut(s) | 2870 |
| MspA1I | CMGCKG | 1 cut(s) | 787 |
| MspI | CCGG | 4 cut(s) | 230, 2392, 3368, 3565 |
| MspR9I | CCNGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| Mva1269I | GAATGC | 4 cut(s) | 1972, 2888, 3604, 3722 |
| MvaI | CCWGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| MvnI | CGCG | 1 cut(s) | 3114 |
| NarI | GGCGCC | 1 cut(s) | 494 |
| NdeI | CATATG | 2 cut(s) | 1003, 1866 |
| NlaIV | GGNNCC | 6 cut(s) | 15, 495, 777, 945, 2494, 3421 |
| NmeAIII | GCCGAG | 2 cut(s) | 3328, 3572 |
| NmuCI | GTSAC | 6 cut(s) | 637, 890, 1320, 2670, 3235, 3357 |
| NsiI | ATGCAT | 2 cut(s) | 1974, 3724 |
| NspI | RCATGY | 5 cut(s) | 591, 681, 1760, 1993, 3841 |
| NspV | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| PaeI | GCATGC | 1 cut(s) | 3841 |
| PaeR7I | CTCGAG | 1 cut(s) | 3049 |
| PaqCI | CACCTGC | 3 cut(s) | 184, 1861, 3677 |
| PceI | AGGCCT | 2 cut(s) | 505, 1116 |
| PciI | ACATGT | 2 cut(s) | 587, 677 |
| PciSI | GCTCTTC | 1 cut(s) | 2082 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 45, 216, 2496 |
| PctI | GAATGC | 4 cut(s) | 1972, 2888, 3604, 3722 |
| PdmI | GAANNNNTTC | 3 cut(s) | 2300, 3000, 3535 |
| PfeI | GAWTC | 8 cut(s) | 235, 1145, 1684, 2189, 2234, 2478, 2932, 3245 |
| PfoI | TCCNGGA | 1 cut(s) | 814 |
| PleI | GAGTC | 9 cut(s) | 1819, 2353, 2506, 2677, 2776, 3313, 3411, 3446, 3511 |
| PluTI | GGCGCC | 1 cut(s) | 497 |
| PmaCI | CACGTG | 1 cut(s) | 1790 |
| PmlI | CACGTG | 1 cut(s) | 1790 |
| PpsI | GAGTC | 9 cut(s) | 1819, 2353, 2506, 2677, 2776, 3313, 3411, 3446, 3511 |
| Ppu21I | YACGTR | 2 cut(s) | 1790, 3032 |
| PscI | ACATGT | 2 cut(s) | 587, 677 |
| PsiI | TTATAA | 2 cut(s) | 1740, 2814 |
| Psp124BI | GAGCTC | 1 cut(s) | 2071 |
| Psp6I | CCWGG | 6 cut(s) | 328, 814, 837, 1111, 3073, 3832 |
| PspCI | CACGTG | 1 cut(s) | 1790 |
| PspFI | CCCAGC | 1 cut(s) | 1574 |
| PspGI | CCWGG | 6 cut(s) | 328, 814, 837, 1111, 3073, 3832 |
| PspN4I | GGNNCC | 6 cut(s) | 15, 495, 777, 945, 2494, 3421 |
| PspPI | GGNCC | 2 cut(s) | 1250, 3419 |
| PspXI | VCTCGAGB | 1 cut(s) | 3049 |
| PsrI | GAACNNNNNNTAC | 6 cut(s) | 667, 699, 2298, 2330, 3268, 3300 |
| PstI | CTGCAG | 1 cut(s) | 1858 |
| PsuI | RGATCY | 6 cut(s) | 5, 1390, 2128, 2710, 3101, 3788 |
| PvuII | CAGCTG | 1 cut(s) | 787 |
| RsaI | GTAC | 4 cut(s) | 777, 1074, 3546, 3701 |
| RsaNI | GTAC | 4 cut(s) | 776, 1073, 3545, 3700 |
| RseI | CAYNNNNRTG | 2 cut(s) | 559, 1137 |
| SacI | GAGCTC | 1 cut(s) | 2071 |
| SapI | GCTCTTC | 1 cut(s) | 2082 |
| Sau96I | GGNCC | 2 cut(s) | 1250, 3419 |
| ScaI | AGTACT | 1 cut(s) | 1074 |
| SchI | GAGTC | 9 cut(s) | 1819, 2354, 2506, 2678, 2777, 3314, 3412, 3446, 3512 |
| ScrFI | CCNGG | 6 cut(s) | 330, 816, 839, 1113, 3075, 3834 |
| SduI | GDGCHC | 3 cut(s) | 1333, 1962, 2071 |
| SfcI | CTRYAG | 2 cut(s) | 1854, 3285 |
| SfoI | GGCGCC | 1 cut(s) | 495 |
| Sfr274I | CTCGAG | 1 cut(s) | 3049 |
| SfuI | TTCGAA | 3 cut(s) | 386, 596, 3478 |
| SinI | GGWCC | 1 cut(s) | 3419 |
| SlaI | CTCGAG | 1 cut(s) | 3049 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 559, 1137 |
| SmlI | CTYRAG | 2 cut(s) | 3049, 3297 |
| SmoI | CTYRAG | 2 cut(s) | 3049, 3297 |
| SnaBI | TACGTA | 1 cut(s) | 3032 |
| SphI | GCATGC | 1 cut(s) | 3841 |
| SseBI | AGGCCT | 2 cut(s) | 505, 1116 |
| SsiI | CCGC | 7 cut(s) | 437, 491, 773, 2655, 2971, 3114, 3390 |
| SspDI | GGCGCC | 1 cut(s) | 493 |
| SspI | AATATT | 2 cut(s) | 541, 1468 |
| SspMI | CTAG | 8 cut(s) | 9, 501, 1197, 1229, 1260, 1643, 2507, 3737 |
| SstI | GAGCTC | 1 cut(s) | 2071 |
| StuI | AGGCCT | 2 cut(s) | 505, 1116 |
| StyD4I | CCNGG | 6 cut(s) | 328, 814, 837, 1111, 3073, 3832 |
| StyI | CCWWGG | 4 cut(s) | 948, 1953, 2686, 2925 |
| TaaI | ACNGT | 5 cut(s) | 669, 2252, 2492, 2609, 3820 |
| TaiI | ACGT | 5 cut(s) | 1000, 1792, 1981, 2301, 3034 |
| TatI | WGTACW | 3 cut(s) | 1072, 3544, 3699 |
| TauI | GCSGC | 3 cut(s) | 494, 2658, 3392 |
| TfiI | GAWTC | 8 cut(s) | 235, 1145, 1684, 2189, 2234, 2478, 2932, 3245 |
| TscAI | CASTG | 3 cut(s) | 1825, 2062, 2839 |
| TseFI | GTSAC | 6 cut(s) | 637, 890, 1320, 2670, 3235, 3357 |
| TseI | GCWGC | 9 cut(s) | 59, 308, 434, 517, 1157, 1309, 2008, 2952, 3632 |
| Tsp45I | GTSAC | 6 cut(s) | 637, 890, 1320, 2670, 3235, 3357 |
| TspGWI | ACGGA | 7 cut(s) | 1149, 1705, 2132, 2269, 2889, 3288, 3665 |
| TspRI | CASTG | 3 cut(s) | 1825, 2062, 2839 |
| VneI | GTGCAC | 1 cut(s) | 1958 |
| VpaK11BI | GGWCC | 1 cut(s) | 3419 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1110, 3831 |
| XapI | RAATTY | 9 cut(s) | 44, 203, 1423, 1542, 1565, 1725, 2566, 2717, 3575 |
| XceI | RCATGY | 5 cut(s) | 591, 681, 1760, 1993, 3841 |
| XhoI | CTCGAG | 1 cut(s) | 3049 |
| XmiI | GTMKAC | 2 cut(s) | 2825, 3034 |
| XmnI | GAANNNNTTC | 3 cut(s) | 2300, 3000, 3535 |
| XspI | CTAG | 8 cut(s) | 9, 501, 1197, 1229, 1260, 1643, 2507, 3737 |
| ZrmI | AGTACT | 1 cut(s) | 1074 |
| Zsp2I | ATGCAT | 2 cut(s) | 1974, 3724 |