Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0002489

Physical Location & Seq

Genomic Coordinates Reverse (-)

49582404 .. 49593952

Gene Structure 11549 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G033620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4131 bp

ATGACCTCCACCGATCCTCACCACATAGTCGACGACGACGACGAAGACGACGATTTCGACTGGGAAGCAGCTGCCAGAGCCGTCGACGTCGTCAGCCAGGCCACCACAAATCCCTCTTCTTCCCATTTTGCCCCTCCTCCCCCAATTCATAAACCCCCATTTCCTCCAATCTCCCACAACAAGCCCGAGACTTCACGGCAGACCACTCTCGATAGGTTCATTGGGAAGCCCGCCCCAAAGCCTCAGCCCGAAATTCGAGACTGCGGCGATGAGAGAGTGTCCCGTGTTCAAATTGATGCCGAAAAAGCCAACACTTGGATGTACCCAGTTAATTTTCCTGGGCGTGACTATCAATTTGCTATCACCAAGACAGCACTTTTTTCGAATACGCTGGTGGCATTGCCAACTGGACTCGGGAAGACGCTTATCGCTGCAGTTGTTATGTATAACTACTTCAGATGGTTCCCTGATGGAAAGATAGTCTTTGCTGCTCCTTCTCGACCACTTGTTACACAACAAATAGAAGCATGCCATAATATTGTAGGGATACCACAAGAATGGACAATTGATATGACAGGTCAGATAAGTCCTACAAAAAGAGCATGCTTTTGGAAAACTAAACGAGTTTTCTTTGTTACTCCACAAGTGCTGGAGAAGGACATTCAGTCTGGAACATGCTCAGTCGAATATTTGGTATGCTTGGTGATTGATGAAGCTCACCGAGCATTAGGGAACTATGCTTCTAGTGTTGCAGTTCGTGAGTTAATGGCGGCACAAGTGCCACTGAGAATTTTAGCTTTGACCGCAACACCAGGATCTAAGCAGCAGACTGTTCAGCAAATTATTGATAATTTGTGTATATCATCACTTGAATACCGTGAAGAAAATGATGCTGATGTCAGCCCTTATGTTCACAACAGGGAGGTAGAGTTGATTAAGGTGGCAATGGGTCAAGATGCTGTGGAGATAAGTAATAAGATATATGATGCAATACGACCATATTTATCCAGGCTTTGTGCGGCTGGAGTTCTCCCAAATAGAGACGCTCAAACGTGGAGCCCAGGCGAGTTACTTAGGTTTAGGGAGAAATTTCGTCTTGCACCTCCTCCAGACTTCCCCCAATTTAAATATGGAGAAATTGAAGGTTGGTATGGAGTTCTTCTTACAGGTTACCACATTCTTAAGCTATTATCAAGCCATGGAATAAGACCAGCATATGACATGCTTGAAGAAAAGTTAAAAAGGGGGTTTTTTGCCAAGTATATGAGTAGAAGTGAAGATGTTCATAAAGCAAAGCTAAGGATGCAGCAAAGTCTGTCTCATGGTGGTCCGAGTCCCAAGTTATCCAAAATGTTAGAAGTATTGCTTGATCATTTCAAAACAAAAGATCCACAAAACTCGAGGGTTATGATTTTCTCAAACTTCAGGGGAAGTGTCAGGGAGATAATGGATGCATTGGCAAACATTGGGAATTTAGTTAAAGCTACCGAGTTTATTGGTCAGAGTTCAGGGAAAATATTAAAAGGCCAGTCACAAAAAGTTCAACAAGCTGTGTTGGAGAAATTTCGTGCTGGTGGATACAATGTCATTGTTGCCACATCAATTGGCGAAGAAGGTTTGGATATAATGGAAGTCGATCTTGTTATATGCTTTGATGCAAATGTATCTCCATTGAGGATGATTCAACGCATGGGGAGAACTGGAAGGAAGCATGATGGACGATTGAAAGGTTACAAGCGGAAGCTAACAAACAGCAAGAATGTGAGCAAACACATGCGAAATGGGGGAATACATAGCTTCAATTTTCATTCTAGCCCGAGGATGATCCCTTACAGTTTCAAACCTGAAGTCCAATATGTTGAGTTTTCAATTGAACAATTCATTCGTCCTGGAAAGAAAGTCAAAGATGATAATGATATTCAGACACCTGTTACTATAGACAAATTAACTGTTGCTGAGACTGGTTTAGTTGCCAAGTATTTCCAGTCACATGACGTAACATGGAAACCATCTCTTATTGCCTTTCCTTACTCCCAAACCTATCCCTCAAGAGTGTATAAAGTAATGCACTCGCCTCGAACAACAATGCTAATTGATACAATGCAATCTTTGCAAGGATTATTCTTTTCCAGTGACAGAAAAATTTCCCTCATTGAGGATGAGGTCTGTTCAGAGGATTGCTTAACAGTTGACACTGTCGAGCAGTGTGACAACAGAGAAGATTTGCTGAATCTTGATGGTTCAAAGAAACAATCACAGGAAATAGGACTGGATTTCGAACTACAAACTACTGGGACATTGGAAACCAAGGAGAAGCATAGCGACCATGATTTATCTGATGAAAATCCTTCTGGTCACTCTTTTCTCTTTGGTTCAGATGTTGTTGATGTTGATGTTGATGCTTCTGGAAAGGTCCGTATCATATCTGTCCCTATTCTTCATGGCAAAAGTATACCAAATACCAAGTTTGCAAGTACAAGCAGTGGTAAGTTGGATCGTTTGAAGCAAAGTTCTTGCCACGTAAGAACATCAGATGAAGAACAAACAGAAGTAACTACTCGAGCTGGAGCTAATGGGGATCCAGAAATGGCTCAGATAGCATATATAAGGAATGATACACCAACTTGGTCTAGATTGTGCGACTCTGAAACCTGGATGGAGACAACAATCAATGCGGATGAAGAGAATCCTCAAAAGCCAATTCTCAAAGGAAACTTTCCAAATGGAGATAGTGCTTGTGAGACGCCTGATGTTCTGAAAAGCAAGGCACCGACATTGCTGGCTGATGAAGACGACAACCTTTTCAGAGATGGTGAGCTTAGTCCAAGGCTTACTAATTTGATCAAAAGTGGAGTTGTTCCAGAGTCACCTATCAATAATAGTGGAATATTAGATACCATAAATAAGTATGTAGTTCCTGATGCTGTTTTGCCTGCCCAATTACCTACGGAACTGATATCGAAGTCTTCAAGTCCTGGGAAAAATGGAAAAGCCACTGATATGGATACCAGTGCATGTGAAACAAATGTGTCCCTTTTTCCTACTGATATTGAAATTCAATCTCCCTTGCACAATAGGAGTACTGCCAGTATAAATGAATCTACCTCCACATCACCGGTTATTGACAGAGGCCAAACTGTTTTAGCAGACCTTACAAACAGTAGCTGTAGCAAAGATTGGTGTATAGGTTCTGGAGACAAGTCACAGAGTGTGAAACAGACACGCAAGTTGAAGAGGTTGCGCAAAGTTGGGGATCATTGGAAAAGTAGGAATCAGGAAAGCATGACAACCAATGATGTCCCAAGAGCAAACCTGACCAGATCTTTTATAAGTACTACTCTTCCTACTAAGCATCTTAGAGGTACCAAAATACATGCAAAGAAGTCGGTTAATGATGTGAGGGAATTCATCGACGAGGAAGCTGAGGTATCTTCAGAGGATATATCTGATGATGAAGAAGATGAACCAGACAACTATTCAAATGATAGTTTCATAGATGACAGGATAAATTTTACGGCTGCAAGTACTCAGGCTGCAACCAGTGGAATTGACATGATGGCAGTGTACAGGCGTTCATTGCTCACCCAATCACCCATGGAGAAGCAGCCAAGCAGTTCAGCCACATTTTCTCCCAATTCTGTGGCAACGATAACTAGGACACCAGAAACTGGAAGTTCTGGTGGAAAAACATCTTTCTCTCTTCAGACTCCTCAAAGCGACTCTACAAATCTGCCAATTGGAATAGATTCAAAGTCTTTCCATAAGAATTGGAACTCTGAAGCCAGAACTTTTACAACTGGTGTTTCTCCAGGATATGAAAGAGATATTGAAAGTCGAAAAAGAAAATCGAGTTTTCATCATTCAAGATCTGTTCCTGCTGTCAACTTGGAGCTAGAATTCTCATTGCAATCAGAGGCTGCAGGCAGAAATATGCAGCACAGTGATGCACATGGGGACATGCTTTATGATGATCAGTTTTATGAGGGTCTTGATCTTGACGCATTGGAAGCACAAGCTACAATTCTTCTTAAGCAGAAATCAGAATTGCCACTGCAGAAGCCACAGATGAGTCAGTTAAACCCAGAAAATCCTACTCTTGACAGTGCTCCATCATTTGATCTTGGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0031297 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043240 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045005 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071704 GO:0071821 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

Helicase_C ResIII

Protein Analysis

1376

Amino Acids

153.17

Weight (kDa)

5.71

Isoelectric Point (pI)

48.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	121 - 275	3.1e-17	DEAD/DEAH box helicase
ResIII	PF04851	127 - 271	1e-17	Type III restriction enzyme, res subunit
Helicase_C	PF00271	454 - 570	1.1e-19	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G36020
fragaria_vesca	FvH4_7g03750
malus_domestica	MD15G1404400.v1.1
prunus_persica	Prupe.1G551100_v2.0.a1 Prupe.2G019600_v2.0.a1 Prupe.2G019700_v2.0.a1
pyrus_communis	pycom15g36170 pycom15g36180
rosa_chinensis	RchiOBHm_Chr7g0228871 RchiOBHm_Chr7g0228881 RchiOBHm_Chr7g0228951
rosa_laevigata	RLG00000001539 RLG00000002150 RLG00000022432
rosa_multiflora	Rmu_sc0000721.1_g000006 Rmu_sc0004517.1_g000001 Rmu_sc0005913.1_g000001 Rmu_sc0005913.1_g000026 Rmu_sc0011218.1_g000006 Rmu_sc0013945.1_g000010
rosa_roxburghii	Rroxscaffold_3G00230830 Rroxscaffold_3G00238230
rosa_rugosa	Rorug07G0252600 Rorug07G0254200
rosa_samantha	Rh7AG405800 Rh7BG333000 Rh7BG385100 Rh7BG385200 Rh7BG385300 Rh7CG423400 Rh7DG341000 Rh7DG399800 Rh7DG399900
rosa_wichuraiana	Rw7G033620 Rw7G033650 Rw7G033670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3329
AatII	GACGTC	1 cut(s)	90
Acc16I	TGCGCA	1 cut(s)	3242
Acc65I	GGTACC	1 cut(s)	3362
AccB1I	GGYRCC	2 cut(s)	2767, 3362
AccB7I	CCANNNNNTGG	2 cut(s)	315, 3668
AccI	GTMKAC	3 cut(s)	30, 84, 2452
AciI	CCGC	7 cut(s)	231, 264, 770, 804, 1019, 1738, 2675
AclWI	GGATC	8 cut(s)	8, 823, 1382, 1819, 2502, 2573, 2586, 3261
AcuI	CTGAAG	6 cut(s)	439, 1408, 1866, 3417, 3686, 3798
AcyI	GRCGYC	2 cut(s)	87, 2744
AdeI	CACNNNGTG	1 cut(s)	3209
AfaI	GTAC	7 cut(s)	323, 2476, 3082, 3334, 3364, 3526, 3566
AfiI	CCNNNNNNNGG	3 cut(s)	315, 2155, 3668
AflII	CTTAAG	2 cut(s)	1181, 4028
AgeI	ACCGGT	1 cut(s)	3115
AhdI	GACNNNNNGTC	1 cut(s)	664
AjnI	CCWGG	9 cut(s)	96, 337, 811, 1007, 1060, 1888, 2651, 2974, 3808
Alw21I	GWGCWC	1 cut(s)	4108
Alw26I	GTCTC	8 cut(s)	182, 252, 1035, 1323, 1952, 2654, 2735, 3189
AlwI	GGATC	8 cut(s)	8, 823, 1382, 1819, 2502, 2573, 2586, 3261
AlwNI	CAGNNNCTG	3 cut(s)	3165, 3668, 3917
Ama87I	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2559
AoxI	GGCC	3 cut(s)	99, 1525, 3130
ArsI	GACNNNNNNTTYG	6 cut(s)	38, 70, 338, 370, 1833, 1865
AsiGI	ACCGGT	1 cut(s)	3115
Asp700I	GAANNNNTTC	3 cut(s)	1281, 2143, 3745
Asp718I	GGTACC	1 cut(s)	3362
AspLEI	GCGC	1 cut(s)	3243
AspS9I	GGNCC	2 cut(s)	1328, 2413
AsuHPI	GGTGA	9 cut(s)	11, 355, 710, 715, 2825, 2859, 3105, 3574, 3582
AsuII	TTCGAA	2 cut(s)	383, 2277
AvaI	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2559
AvaII	GGWCC	2 cut(s)	1328, 2413
BamHI	GGATCC	1 cut(s)	2578
BanI	GGYRCC	2 cut(s)	2767, 3362
BanII	GRGCYC	1 cut(s)	1061
BarI	GAAGNNNNNNTAC	2 cut(s)	437, 469
BbsI	GAAGAC	4 cut(s)	51, 425, 2796, 2958
Bbv12I	GWGCWC	1 cut(s)	4108
BbvCI	CCTCAGC	2 cut(s)	243, 3423
BccI	CCATC	9 cut(s)	453, 464, 1709, 2017, 2231, 2650, 2804, 3550, 4117
BceAI	ACGGC	3 cut(s)	65, 212, 3531
BcgI	CGANNNNNNTGC	6 cut(s)	363, 397, 2057, 2091, 3366, 3400
BciT130I	CCWGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
BciVI	GTATCC	3 cut(s)	540, 1571, 2998
BclI	TGATCA	3 cut(s)	1369, 2841, 3970
BcoDI	GTCTC	8 cut(s)	182, 252, 1035, 1323, 1952, 2654, 2735, 3189
BfaI	CTAG	5 cut(s)	744, 1812, 2631, 3654, 3893
BfmI	CTRYAG	5 cut(s)	432, 1935, 3166, 3918, 4052
BfrI	CTTAAG	2 cut(s)	1181, 4028
BfuI	GTATCC	3 cut(s)	540, 1571, 2998
BglII	AGATCT	2 cut(s)	3320, 3866
BmcAI	AGTACT	3 cut(s)	3082, 3334, 3526
Bme1390I	CCNGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
Bme18I	GGWCC	2 cut(s)	1328, 2413
BmeRI	GACNNNNNGTC	1 cut(s)	664
BmeT110I	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2559
BmgT120I	GGNCC	2 cut(s)	1328, 2413
BmiI	GGNNCC	5 cut(s)	464, 1058, 2580, 2769, 3364
BmrFI	CCNGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
BmrI	ACTGGG	3 cut(s)	70, 320, 2301
BmuI	ACTGGG	3 cut(s)	70, 320, 2301
BpiI	GAAGAC	4 cut(s)	51, 425, 2796, 2958
BpmI	CTGGAG	6 cut(s)	671, 1044, 1092, 2586, 3213, 3792
Bpu10I	CCTNAGC	3 cut(s)	243, 1298, 3423
Bpu14I	TTCGAA	2 cut(s)	383, 2277
BpuEI	CTTGAG	1 cut(s)	2032
BsaAI	YACGTR	1 cut(s)	2521
BsaBI	GATNNNNATC	1 cut(s)	2343
BsaHI	GRCGYC	2 cut(s)	87, 2744
BsaJI	CCNNGG	8 cut(s)	338, 1060, 1198, 1817, 2307, 2825, 2975, 3594
BsaWI	WCCGGW	1 cut(s)	3115
BsaXI	ACNNNNNCTCC	4 cut(s)	914, 944, 3613, 3643
Bsc4I	CCNNNNNNNGG	3 cut(s)	315, 2155, 3668
Bse118I	RCCGGY	1 cut(s)	3115
Bse3DI	GCAATG	5 cut(s)	398, 951, 2774, 3575, 3902
Bse8I	GATNNNNATC	1 cut(s)	2343
BseBI	CCWGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
BseDI	CCNNGG	8 cut(s)	338, 1060, 1198, 1817, 2307, 2825, 2975, 3594
BseGI	GGATG	8 cut(s)	324, 1308, 1456, 1683, 1827, 2164, 2661, 2683
BseJI	GATNNNNATC	1 cut(s)	2343
BseLI	CCNNNNNNNGG	3 cut(s)	315, 2155, 3668
BseMI	GCAATG	5 cut(s)	398, 951, 2774, 3575, 3902
BseMII	CTCAG	7 cut(s)	257, 693, 776, 1947, 2606, 3414, 3542
BseRI	GAGGAG	3 cut(s)	126, 1095, 3699
BshFI	GGCC	3 cut(s)	101, 1527, 3132
BshNI	GGYRCC	2 cut(s)	2767, 3362
BshTI	ACCGGT	1 cut(s)	3115
BsiHKAI	GWGCWC	1 cut(s)	4108
BsiHKCI	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2559
BsiSI	CCGG	1 cut(s)	3116
BslFI	GGGAC	7 cut(s)	265, 1320, 2308, 2414, 3016, 3284, 3968
BslI	CCNNNNNNNGG	3 cut(s)	315, 2155, 3668
BsmAI	GTCTC	8 cut(s)	182, 252, 1035, 1323, 1952, 2654, 2735, 3189
BsmBI	CGTCTC	2 cut(s)	1035, 2735
BsmFI	GGGAC	7 cut(s)	265, 1320, 2308, 2414, 3016, 3284, 3968
BsnI	GGCC	3 cut(s)	101, 1527, 3132
BsoBI	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2559
Bsp119I	TTCGAA	2 cut(s)	383, 2277
Bsp1286I	GDGCHC	2 cut(s)	1061, 4108
Bsp1407I	TGTACA	1 cut(s)	3564
Bsp19I	CCATGG	2 cut(s)	1198, 3594
BspACI	CCGC	7 cut(s)	231, 264, 770, 804, 1019, 1738, 2675
BspANI	GGCC	3 cut(s)	101, 1527, 3132
BspCNI	CTCAG	7 cut(s)	256, 692, 777, 1948, 2605, 3415, 3541
BspLI	GGNNCC	5 cut(s)	464, 1058, 2580, 2769, 3364
BspMAI	CTGCAG	3 cut(s)	436, 3922, 4056
BspPI	GGATC	8 cut(s)	8, 823, 1382, 1819, 2502, 2573, 2586, 3261
BspT104I	TTCGAA	2 cut(s)	383, 2277
BspT107I	GGYRCC	2 cut(s)	2767, 3362
BspTI	CTTAAG	2 cut(s)	1181, 4028
BsrDI	GCAATG	5 cut(s)	398, 951, 2774, 3575, 3902
BsrFI	RCCGGY	1 cut(s)	3115
BsrGI	TGTACA	1 cut(s)	3564
BssAI	RCCGGY	1 cut(s)	3115
BssECI	CCNNGG	8 cut(s)	338, 1060, 1198, 1817, 2307, 2825, 2975, 3594
BssNAI	GTATAC	1 cut(s)	2453
BssNI	GRCGYC	2 cut(s)	87, 2744
BssT1I	CCWWGG	4 cut(s)	1198, 2307, 2825, 3594
Bst1107I	GTATAC	1 cut(s)	2453
Bst2UI	CCWGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
Bst6I	CTCTTC	5 cut(s)	121, 2676, 3227, 3345, 3705
BstACI	GRCGYC	2 cut(s)	87, 2744
BstAFI	CTTAAG	2 cut(s)	1181, 4028
BstAPI	GCANNNNNTGC	1 cut(s)	2110
BstAUI	TGTACA	1 cut(s)	3564
BstBAI	YACGTR	1 cut(s)	2521
BstBI	TTCGAA	2 cut(s)	383, 2277
BstC8I	GCNNGC	6 cut(s)	231, 529, 604, 2781, 2934, 3922
BstDSI	CCRYGG	2 cut(s)	1198, 3594
BstEII	GGTNACC	1 cut(s)	1169
BstENI	CCTNNNNNAGG	1 cut(s)	2153
BstF5I	GGATG	8 cut(s)	324, 1308, 1456, 1683, 1827, 2164, 2661, 2683
BstHHI	GCGC	1 cut(s)	3243
BstMAI	GTCTC	8 cut(s)	182, 252, 1035, 1323, 1952, 2654, 2735, 3189
BstMWI	GCNNNNNNNGC	9 cut(s)	77, 305, 722, 803, 1303, 2110, 2597, 3577, 4007
BstNI	CCWGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
BstNSI	RCATGY	8 cut(s)	531, 606, 678, 1225, 1777, 3018, 3377, 3961
BstPI	GGTNACC	1 cut(s)	1169
BstSCI	CCNGG	9 cut(s)	96, 337, 811, 1007, 1060, 1888, 2651, 2974, 3808
BstSFI	CTRYAG	5 cut(s)	432, 1935, 3166, 3918, 4052
BstV2I	GAAGAC	4 cut(s)	51, 425, 2796, 2958
BstX2I	RGATCY	5 cut(s)	815, 1387, 2578, 3320, 3866
BstXI	CCANNNNNNTGG	3 cut(s)	558, 3001, 3640
BstYI	RGATCY	5 cut(s)	815, 1387, 2578, 3320, 3866
BstZ17I	GTATAC	1 cut(s)	2453
BsuI	GTATCC	3 cut(s)	540, 1571, 2998
BsuRI	GGCC	3 cut(s)	101, 1527, 3132
BtgI	CCRYGG	2 cut(s)	1198, 3594
BtgZI	GCGATG	1 cut(s)	282
BtsCI	GGATG	8 cut(s)	324, 1308, 1456, 1683, 1827, 2164, 2661, 2683
BtsI	GCAGTG	4 cut(s)	2210, 2488, 3567, 4049
Cac8I	GCNNGC	6 cut(s)	231, 529, 604, 2781, 2934, 3922
CaiI	CAGNNNCTG	3 cut(s)	3165, 3668, 3917
CfoI	GCGC	1 cut(s)	3243
Cfr10I	RCCGGY	1 cut(s)	3115
Cfr13I	GGNCC	2 cut(s)	1328, 2413
CseI	GACGC	4 cut(s)	430, 1052, 2752, 4007
Csp6I	GTAC	7 cut(s)	322, 2475, 3081, 3333, 3363, 3525, 3565
CspAI	ACCGGT	1 cut(s)	3115
CspCI	CAANNNNNGTGG	4 cut(s)	1585, 1620, 2863, 2898
CviQI	GTAC	7 cut(s)	322, 2475, 3081, 3333, 3363, 3525, 3565
DraI	TTTAAA	1 cut(s)	1126
DraIII	CACNNNGTG	1 cut(s)	3209
DriI	GACNNNNNGTC	1 cut(s)	664
Eam1104I	CTCTTC	5 cut(s)	121, 2676, 3227, 3345, 3705
Eam1105I	GACNNNNNGTC	1 cut(s)	664
EarI	CTCTTC	5 cut(s)	121, 2676, 3227, 3345, 3705
Eco130I	CCWWGG	4 cut(s)	1198, 2307, 2825, 3594
Eco24I	GRGCYC	1 cut(s)	1061
Eco32I	GATATC	1 cut(s)	2958
Eco47I	GGWCC	2 cut(s)	1328, 2413
Eco57I	CTGAAG	6 cut(s)	439, 1408, 1866, 3417, 3686, 3798
Eco88I	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2559
Eco91I	GGTNACC	1 cut(s)	1169
EcoNI	CCTNNNNNAGG	1 cut(s)	2153
EcoO65I	GGTNACC	1 cut(s)	1169
EcoRI	GAATTC	2 cut(s)	3404, 3896
EcoRII	CCWGG	9 cut(s)	96, 337, 811, 1007, 1060, 1888, 2651, 2974, 3808
EcoRV	GATATC	1 cut(s)	2958
EcoT14I	CCWWGG	4 cut(s)	1198, 2307, 2825, 3594
EcoT22I	ATGCAT	1 cut(s)	1456
EcoT38I	GRGCYC	1 cut(s)	1061
ErhI	CCWWGG	4 cut(s)	1198, 2307, 2825, 3594
Esp3I	CGTCTC	2 cut(s)	1035, 2735
FalI	AAGNNNNNCTT	6 cut(s)	467, 499, 1350, 1382, 1623, 1655
FaqI	GGGAC	7 cut(s)	265, 1320, 2308, 2414, 3016, 3284, 3968
FauI	CCCGC	1 cut(s)	238
FauNDI	CATATG	1 cut(s)	1216
FbaI	TGATCA	3 cut(s)	1369, 2841, 3970
FblI	GTMKAC	3 cut(s)	30, 84, 2452
FokI	GGATG	8 cut(s)	331, 1315, 1463, 1690, 1834, 2171, 2668, 2690
FriOI	GRGCYC	1 cut(s)	1061
FspBI	CTAG	5 cut(s)	744, 1812, 2631, 3654, 3893
FspI	TGCGCA	1 cut(s)	3242
GlaI	GCGC	1 cut(s)	3242
GsuI	CTGGAG	6 cut(s)	671, 1044, 1092, 2586, 3213, 3792
HaeIII	GGCC	3 cut(s)	101, 1527, 3132
HapII	CCGG	1 cut(s)	3116
HgaI	GACGC	4 cut(s)	430, 1052, 2752, 4007
HhaI	GCGC	1 cut(s)	3243
Hin1I	GRCGYC	2 cut(s)	87, 2744
Hin6I	GCGC	1 cut(s)	3241
HinP1I	GCGC	1 cut(s)	3241
HincII	GTYRAC	4 cut(s)	31, 85, 2191, 3883
HindII	GTYRAC	4 cut(s)	31, 85, 2191, 3883
HpaII	CCGG	1 cut(s)	3116
HphI	GGTGA	9 cut(s)	11, 355, 710, 715, 2825, 2859, 3105, 3574, 3582
Hpy166II	GTNNAC	7 cut(s)	31, 85, 913, 2191, 2453, 3565, 3883
Hpy8I	GTNNAC	7 cut(s)	31, 85, 913, 2191, 2453, 3565, 3883
Hpy99I	CGWCG	9 cut(s)	35, 38, 41, 44, 53, 86, 89, 92, 3416
HpyAV	CCTTC	6 cut(s)	504, 649, 1136, 1607, 1698, 2358
HpyCH4IV	ACGT	4 cut(s)	87, 1052, 1995, 2520
HpyF10VI	GCNNNNNNNGC	9 cut(s)	77, 305, 722, 803, 1303, 2110, 2597, 3577, 4007
HpySE526I	ACGT	4 cut(s)	87, 1052, 1995, 2520
Hsp92I	GRCGYC	2 cut(s)	87, 2744
HspAI	GCGC	1 cut(s)	3241
KpnI	GGTACC	1 cut(s)	3366
Ksp22I	TGATCA	3 cut(s)	1369, 2841, 3970
LmnI	GCTCC	5 cut(s)	496, 1056, 2567, 3889, 4111
MaeI	CTAG	5 cut(s)	744, 1812, 2631, 3654, 3893
MaeII	ACGT	4 cut(s)	87, 1052, 1995, 2520
MfeI	CAATTG	4 cut(s)	564, 1602, 1869, 3735
MflI	RGATCY	5 cut(s)	815, 1387, 2578, 3320, 3866
MhlI	GDGCHC	2 cut(s)	1061, 4108
MlyI	GAGTC	7 cut(s)	405, 1342, 2636, 2873, 3700, 3713, 4078
MmeI	TCCRAC	2 cut(s)	1536, 2472
Mph1103I	ATGCAT	1 cut(s)	1456
MroXI	GAANNNNTTC	3 cut(s)	1281, 2143, 3745
MslI	CAYNNNNRTG	4 cut(s)	317, 556, 2999, 3942
MspA1I	CMGCKG	1 cut(s)	71
MspCI	CTTAAG	2 cut(s)	1181, 4028
MspI	CCGG	1 cut(s)	3116
MspR9I	CCNGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
MunI	CAATTG	4 cut(s)	564, 1602, 1869, 3735
MvaI	CCWGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
MwoI	GCNNNNNNNGC	9 cut(s)	77, 305, 722, 803, 1303, 2110, 2597, 3577, 4007
NcoI	CCATGG	2 cut(s)	1198, 3594
NdeI	CATATG	1 cut(s)	1216
NlaIV	GGNNCC	5 cut(s)	464, 1058, 2580, 2769, 3364
NmuCI	GTSAC	8 cut(s)	344, 1530, 1986, 2132, 2207, 2354, 2865, 3201
NsbI	TGCGCA	1 cut(s)	3242
NsiI	ATGCAT	1 cut(s)	1456
NspI	RCATGY	8 cut(s)	531, 606, 678, 1225, 1777, 3018, 3377, 3961
NspV	TTCGAA	2 cut(s)	383, 2277
PaeI	GCATGC	2 cut(s)	531, 606
PaeR7I	CTCGAG	2 cut(s)	1399, 2559
PcsI	WCGNNNNNNNCGW	5 cut(s)	36, 39, 45, 48, 54
PdmI	GAANNNNTTC	3 cut(s)	1281, 2143, 3745
PfeI	GAWTC	6 cut(s)	1681, 2230, 2686, 3098, 3271, 3746
PflFI	GACNNNGTC	2 cut(s)	89, 2195
PflMI	CCANNNNNTGG	2 cut(s)	315, 3668
PfoI	TCCNGGA	2 cut(s)	1888, 3808
PinAI	ACCGGT	1 cut(s)	3115
PleI	GAGTC	7 cut(s)	405, 1341, 2636, 2872, 3700, 3713, 4077
PpsI	GAGTC	7 cut(s)	405, 1341, 2636, 2872, 3700, 3713, 4077
Ppu21I	YACGTR	1 cut(s)	2521
PsiI	TTATAA	1 cut(s)	3329
Psp6I	CCWGG	9 cut(s)	96, 337, 811, 1007, 1060, 1888, 2651, 2974, 3808
PspEI	GGTNACC	1 cut(s)	1169
PspGI	CCWGG	9 cut(s)	96, 337, 811, 1007, 1060, 1888, 2651, 2974, 3808
PspN4I	GGNNCC	5 cut(s)	464, 1058, 2580, 2769, 3364
PspPI	GGNCC	2 cut(s)	1328, 2413
PspXI	VCTCGAGB	2 cut(s)	1399, 2559
PstI	CTGCAG	3 cut(s)	436, 3922, 4056
PstNI	CAGNNNCTG	3 cut(s)	3165, 3668, 3917
PsuI	RGATCY	5 cut(s)	815, 1387, 2578, 3320, 3866
PsyI	GACNNNGTC	2 cut(s)	89, 2195
PvuII	CAGCTG	1 cut(s)	71
RsaI	GTAC	7 cut(s)	323, 2476, 3082, 3334, 3364, 3526, 3566
RsaNI	GTAC	7 cut(s)	322, 2475, 3081, 3333, 3363, 3525, 3565
RseI	CAYNNNNRTG	4 cut(s)	317, 556, 2999, 3942
SalI	GTCGAC	2 cut(s)	29, 83
Sau96I	GGNCC	2 cut(s)	1328, 2413
ScaI	AGTACT	3 cut(s)	3082, 3334, 3526
SchI	GAGTC	7 cut(s)	405, 1342, 2636, 2873, 3700, 3713, 4078
ScrFI	CCNGG	9 cut(s)	98, 339, 813, 1009, 1062, 1890, 2653, 2976, 3810
SduI	GDGCHC	2 cut(s)	1061, 4108
SfcI	CTRYAG	5 cut(s)	432, 1935, 3166, 3918, 4052
Sfr274I	CTCGAG	2 cut(s)	1399, 2559
SfuI	TTCGAA	2 cut(s)	383, 2277
SgrDI	CGTCGACG	1 cut(s)	83
SinI	GGWCC	2 cut(s)	1328, 2413
SlaI	CTCGAG	2 cut(s)	1399, 2559
SmiI	ATTTAAAT	1 cut(s)	1126
SmiMI	CAYNNNNRTG	4 cut(s)	317, 556, 2999, 3942
SmlI	CTYRAG	5 cut(s)	1181, 1399, 2047, 2559, 4028
SmoI	CTYRAG	5 cut(s)	1181, 1399, 2047, 2559, 4028
SphI	GCATGC	2 cut(s)	531, 606
SsiI	CCGC	7 cut(s)	231, 264, 770, 804, 1019, 1738, 2675
SspI	AATATT	4 cut(s)	538, 689, 1518, 2889
SspMI	CTAG	5 cut(s)	744, 1812, 2631, 3654, 3893
StyD4I	CCNGG	9 cut(s)	96, 337, 811, 1007, 1060, 1888, 2651, 2974, 3808
StyI	CCWWGG	4 cut(s)	1198, 2307, 2825, 3594
SwaI	ATTTAAAT	1 cut(s)	1126
TaiI	ACGT	4 cut(s)	90, 1055, 1998, 2523
TatI	WGTACW	5 cut(s)	2474, 3080, 3332, 3524, 3564
TauI	GCSGC	3 cut(s)	267, 773, 1022
TfiI	GAWTC	6 cut(s)	1681, 2230, 2686, 3098, 3271, 3746
TseFI	GTSAC	8 cut(s)	344, 1530, 1986, 2132, 2207, 2354, 2865, 3201
Tsp45I	GTSAC	8 cut(s)	344, 1530, 1986, 2132, 2207, 2354, 2865, 3201
TspGWI	ACGGA	2 cut(s)	2405, 2963
Tth111I	GACNNNGTC	2 cut(s)	89, 2195
Van91I	CCANNNNNTGG	2 cut(s)	315, 3668
Vha464I	CTTAAG	2 cut(s)	1181, 4028
VpaK11BI	GGWCC	2 cut(s)	1328, 2413
XagI	CCTNNNNNAGG	1 cut(s)	2153
XbaI	TCTAGA	1 cut(s)	2630
XceI	RCATGY	8 cut(s)	531, 606, 678, 1225, 1777, 3018, 3377, 3961
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1127
XhoI	CTCGAG	2 cut(s)	1399, 2559
XmiI	GTMKAC	3 cut(s)	30, 84, 2452
XmnI	GAANNNNTTC	3 cut(s)	1281, 2143, 3745
XspI	CTAG	5 cut(s)	744, 1812, 2631, 3654, 3893
ZraI	GACGTC	1 cut(s)	88
ZrmI	AGTACT	3 cut(s)	3082, 3334, 3526
Zsp2I	ATGCAT	1 cut(s)	1456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.