Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002489

Physical Location & Seq

Genomic Coordinates Forward (+)

50529108 .. 50541496

Gene Structure 12389 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1404400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4203 bp

ATGACTTCCACCAATCCTCACCGAGTCATCGACGACGACGACGATGATTTTGATTGGGAAGCGGCGGCGAAAGAAATCGACGTGGTTTACCAGACCCAGGCTTCAAGGCCCTCCTCTACTTCAGATCAATCTTCCCATTTTGCCCCTCCTCCCAGTAACATTAACACCCCAGTGCAAAATCCGATTGCCCCGATCCCTGGCAACAAGAAGAAGGCCGGCACTTCCCGGCAGAGCACCCTCGACAAGTTCATGGGAAGGCCTCCGCCAGGGATTCGAGACGTCGAAGAACGAAACCTGGATTGTAGTGAGGGTGATGGGAGAGTGTCCTGTGTTCCAATTGATGCTGAGGCAGCTAAAACTTGGATGTACCCAGTTAATTTTCCTCGGCGTGATTATCAATTTTCTATTACCCAGACGGCATTATTTTCAAATACATTGGTGGTACTGCCAACTGGACTTGGGAAAACGCTAATTGCTGCAGTTGTTATGTATAACTATTTCAGATGGTTCCCTAATGGAAAAATAGTCTTTGCTGCTCCTTCTCGACCACTTGTTGTGCAACAAATACAAGCGTGTCATAATATTGTAGGAATACCACAGGAATGGACAATTGATATGACAGGTCAGACAAGTCCTACTAAAAGAGCTTGCTTGTGGAAAACTAAACGAGTTTTTTTTGTTACTCCACAAGTGCTGGAGAAGGACATTCAGTCTGGCACATGTGCAGTAGAATACCTGGTATGTTTGGTGATTGATGAAGCTCACCGAGCATTAGGAAACTATTCTTACAGTGTTGCAGTTCGTGAGTTAATGGCTGAACAAGTGCAACTGCGAATTTTAGCTTTGAGTGCAACACCGGGATCAAAGCAGCAGACTGTCCAGCAAGTTATTGATAACTTATATATATCAACACTTCAATATCGCAATGAAGATGACCCTGATGTCAAACCATATGTCCACAACAGGGACGTAGAGTTGATTCAGGTTGCAATGGGTCAAGCTGCTGTTGAGATAGACAATGAGCTCTTGGAAGCAATGCATCCATTTGCATCCAGGCTTTGTGCTATTGGAGTTCTCCCAACTCGAGACATTCAGAATTTAAGCCCTTGTCTATTACTTAATTCAAGAGAGAAATTTCGTCAAGCACCTCCAGACGTCTCCCAAATTAAGCCTGGAGATATTGAAGGTTTCTTTGGAACACTGCTTACACTTTACCACATTCGTAAACTAATATCAAGCCATGGAATAAGGCCAGCATATGAAATGCTTGAAGAAAAATTAAAACAAGGGTCTTTTGCAAGGTATATGAGCAGAAATGAAGATATTTGTAAAGCAAAGCTCATAATGCAGCAAAGTTTGTCACATGGTGCTCCGAGTCCCAAATTGTCCAAAATGTTAGAAGTACTGCATGATCATTTCAAAACAAATGATCCACAGAAATCAAGGGTTATAATTTTCTCAAATTTTCGGGGAAGTGTCAGGGACATAATGGATGCATTAGCAAACCTTGGCAATTTAGTCAAAGCTACCCAGTTTATTGGTCAAAGTTCAGGGAAAACATTAAAAGGCCAGTCACAAAAAGTTCAACAAGCTGTTTTAGAGAAATTTCGTGCTGGTGGATACAATGTCATTGTTGCCACATCTATTGGTGAAGAAGGCCTGGATATTATGGAAGTTGATCTGGTTATATGCTTTGATGCTAACGTATCACCATTGAGAATGATTCAACGCATGGGGAGAACTGGAAGGAAGCATGATGGACGAGTTGTAGTTCTAGCCTGTGAAGGGTCAGAGTTGAAGGGTTACCATCGTAAGAAAGGAAACAGTAAGAATATGATGAAACACATGCGAAATGGGGGAATAAATAGCTTCAATTTCCATTCTAGTCCAAGGATGGTGGGATCTTCTAACAGATGCTGCATCTTTATAACTTTTAATCCTGCAGTCTATTTTTTAGCTGTTGATTGCATCAAATTTTATTGCAGATTCCTCATATTTTCAAACCCGAAGTCCATGATTGATGATAACACTGTTCAGACTCCTGTGATCGCAGAAACGTTAACTGTATCTGAGACTAACTTAATTGCCAAGTATTTCCACCCTCATGGACTTACATGGAAACCATCTCTTATTGCCTTTCCTCACTTTCAAACGTATCCGTCAAGAGTATATAAAGTAATGCATTCACGTCGGACAACCATGCTGATTGATATGATGCAATGTTTGCAAGGACTAACCTTTTCCAGGGACAGCAATATCTCTCTTGTTGAGGATGAATTCTATGCAGAGGGAATTGACACTACCGAGCCACATGTCATTGACGCTGCTGAACATCATGACAACAACAGACAAGGTTTTCTGAATCCTGATGATTCTTTAGAGAAACAGTCGGAAAGAAAAGTATTGGATTCTGAATTATCACCTAATAGTACATTGAAAACCAAGGAGAAGCATTACAGACTTAATTTCCCTGGTGAAAATCTTTATGAGCACTCTTACCTCTTTGGTTCAGATGTTGTAAATGTGGATGCTTCTGGAAAGGTCTTAATCACATCTGTTCCTGTATTTCGATGGAAAGACTTGTCACATTCCATGTTCACAAGTGAAAGTAGTATTAAGTTGGATAGCTTGAAGCAAAATTCTTACCATGGAAGGACTTCAGATGAAGACCACACAGAACTAACTACTCAAGGTGGTGCTAGTGGAGATGTTAAAACTATTCAGATGAAAAGCATAAAATATGAGAATTCACTTAGCTCTAGATTGTGCAAGTCTGAAATCTGGACAGAGAAAACATTCAATGGTGCTGAAAAAATTCTTCAAACTACAATTTTGAAGGGAGATTTGTTAAGTAAAGGAGATAGTGTTTCCGACACACCTGATGTTCTGGAAAGCAAGGGATCATTGTTGCTCACAGATGAAGACAATAGTATTTTGAGAGATGGTGAGCTCAGTCCACGGCTTACTAATTTAATGAAAAGTGGAGTTGTTCCAGAGTCTCCTATCAACACTAGTGGACCATCAAATAACGCAAATGAGTGTGCAGTTCCTGTCCCCGTTTCACCTGCCCAATTACATCCTGAAAATAGTGAAAAGGTCAGTATGGAAAACAATGCCTGTGGAGGAAAAGTATCTGTTTCTTCTATGGATACTGAAATCCGAACTCCTTTCCATAACAAGAGTAATAGTGCCAGTATAAGAGGTTGTACCTCCGCATCACCAATTAGTGGAAGAGCCAATACTGTTTTAGCTGACCTTACAAATAGTTGTGGCAAAGATTGGAATTTAATTTCCGGAGACAAGTCGGAAAGTATAAAAGAGGCACGCAAGTTCAGAAGGTTGCACAAAGTTGGGGATCGTTGGAAAAACAGGAAACCAGAAAGCGTGACAAATAATATGGGGTCAACAAAAAACCTTGCCAGATCATTTTCTAAAACTGGTTCCCCCCATTCGAAGCACAATAGAGGTAAAAAGAATTCAGTTGATGATGTAAGGGCCTTCATCGAGGAGGAAGCTGAGGTATCTTCAGAAGCTGGTATGTCTGATGACGAGGAAGATGATCAAGACAACTATTCAAATGATAGTTTCATAGATGACAGAATAAATCCTACAGCTGCGGCTACTCAGTCTGCAAGTGGAGGAAATGATATGATGGCAATATACAGACGTTCATTGCTCACCCAATCACCCATGGAGAGGCAGCCAGTTAGTTCTGCGGGATATTCACCTGATTCTGTGGCAGCGACAACGAGGACAACTGAAACTGGAAGTTCCTGTGGCAAAACATCGTTCTCTCTCCAAACGCCTCAAAGTGATGGTACAAATCAGTCAACCAGGATGGATTCAAAGTCCTTGGAAATGAACCGGGTAAGGGTAGACATACCTTGTACTGGTGGTGCTTCCCCAGAATACGAGAGAGATGTTGAAAGCCGTAAAAGAAAATTGAGTTGTCATCATTCAAGATATCCTGCAGTCAACTTGGAGCGAGAGTTCTCGCTCGAGTCAGAGGCTGCAGGCAGAGACTTGCAGCGCATTGATGCAAACGATGACATGTTTGACGATCAGTTTTTCGAGGGTCTTGATCTTGACGCAATGGAAGCGCAAGCTACATTGCTTCTTAAACAGAAAACAGAAGCGCCAAGGCCGCAGGAGCAGCATCTGGTTCCCAAGTTGTTCGAGCCCACATTTGATCTTGGTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0031297 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043240 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045005 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071704 GO:0071821 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

Helicase_C ResIII

Protein Analysis

1401

Amino Acids

155.82

Weight (kDa)

6.2

Isoelectric Point (pI)

46.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	138 - 290	1.1e-18	DEAD/DEAH box helicase
ResIII	PF04851	142 - 286	2.1e-16	Type III restriction enzyme, res subunit
Helicase_C	PF00271	468 - 584	1.8e-19	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G36020
fragaria_vesca	FvH4_7g03750
malus_domestica	MD15G1404400.v1.1
prunus_persica	Prupe.1G551100_v2.0.a1 Prupe.2G019600_v2.0.a1 Prupe.2G019700_v2.0.a1
pyrus_communis	pycom15g36170 pycom15g36180
rosa_chinensis	RchiOBHm_Chr7g0228871 RchiOBHm_Chr7g0228881 RchiOBHm_Chr7g0228951
rosa_laevigata	RLG00000001539 RLG00000002150 RLG00000022432
rosa_multiflora	Rmu_sc0000721.1_g000006 Rmu_sc0004517.1_g000001 Rmu_sc0005913.1_g000001 Rmu_sc0005913.1_g000026 Rmu_sc0011218.1_g000006 Rmu_sc0013945.1_g000010
rosa_roxburghii	Rroxscaffold_3G00230830 Rroxscaffold_3G00238230
rosa_rugosa	Rorug07G0252600 Rorug07G0254200
rosa_samantha	Rh7AG405800 Rh7BG333000 Rh7BG385100 Rh7BG385200 Rh7BG385300 Rh7CG423400 Rh7DG341000 Rh7DG399800 Rh7DG399900
rosa_wichuraiana	Rw7G033620 Rw7G033650 Rw7G033670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1451, 1928
AarI	CACCTGC	1 cut(s)	3073
AasI	GACNNNNNNGTC	1 cut(s)	941
AatII	GACGTC	2 cut(s)	282, 1158
Acc36I	ACCTGC	1 cut(s)	3073
AccB7I	CCANNNNNTGG	1 cut(s)	3227
AccI	GTMKAC	1 cut(s)	3876
AccIII	TCCGGA	1 cut(s)	3293
AciI	CCGC	7 cut(s)	62, 65, 263, 3213, 3617, 3716, 4145
AclI	AACGTT	1 cut(s)	2057
AclWI	GGATC	6 cut(s)	187, 868, 1424, 1909, 2908, 3363
AcuI	CTGAAG	3 cut(s)	105, 2643, 3510
AcyI	GRCGYC	2 cut(s)	279, 1155
AdeI	CACNNNGTG	1 cut(s)	1367
AfaI	GTAC	7 cut(s)	368, 444, 1404, 2431, 3208, 3820, 3889
AflIII	ACRYGT	3 cut(s)	719, 2311, 4050
AhdI	GACNNNNNGTC	1 cut(s)	709
AhlI	ACTAGT	1 cut(s)	3011
AjiI	CACGTC	2 cut(s)	82, 2189
AjuI	GAANNNNNNNTTGG	2 cut(s)	1176, 1208
AleI	CACNNNNGTG	1 cut(s)	170
AloI	GAACNNNNNNTCC	2 cut(s)	3974, 4006
Alw21I	GWGCWC	5 cut(s)	236, 1026, 1372, 2493, 2952
Alw26I	GTCTC	7 cut(s)	270, 1080, 1162, 2066, 3003, 3291, 4014
AlwI	GGATC	6 cut(s)	187, 868, 1424, 1909, 2908, 3363
AlwNI	CAGNNNCTG	5 cut(s)	1917, 3050, 3533, 4010, 4159
Ama87I	CYCGRG	2 cut(s)	1083, 3998
Aor13HI	TCCGGA	1 cut(s)	3293
AoxI	GGCC	8 cut(s)	107, 213, 257, 1250, 1567, 1657, 3495, 4142
ArsI	GACNNNNNNTTYG	6 cut(s)	32, 64, 939, 971, 1994, 2026
Asp700I	GAANNNNTTC	4 cut(s)	781, 2656, 2795, 2814
AspLEI	GCGC	3 cut(s)	4032, 4102, 4138
AspS9I	GGNCC	3 cut(s)	108, 3017, 3495
AsuC2I	CCSGG	3 cut(s)	226, 858, 3866
AsuII	TTCGAA	1 cut(s)	3452
AvaI	CYCGRG	2 cut(s)	1083, 3998
AvaII	GGWCC	1 cut(s)	3017
BanII	GRGCYC	3 cut(s)	1026, 2952, 4182
BarI	GAAGNNNNNNTAC	2 cut(s)	3223, 3255
BbsI	GAAGAC	2 cut(s)	2673, 2928
Bbv12I	GWGCWC	5 cut(s)	236, 1026, 1372, 2493, 2952
BbvCI	CCTCAGC	2 cut(s)	345, 3516
BceAI	ACGGC	3 cut(s)	432, 2975, 3915
BcgI	CGANNNNNNTGC	2 cut(s)	2171, 2205
BciVI	GTATCC	3 cut(s)	1613, 2166, 3142
BclI	TGATCA	2 cut(s)	1411, 3559
BcnI	CCSGG	3 cut(s)	226, 858, 3866
BcoDI	GTCTC	7 cut(s)	270, 1080, 1162, 2066, 3003, 3291, 4014
BcuI	ACTAGT	1 cut(s)	3011
BfaI	CTAG	5 cut(s)	1775, 1884, 2700, 2760, 3012
BfmI	CTRYAG	5 cut(s)	477, 1941, 3609, 3969, 4011
BfoI	RGCGCY	1 cut(s)	4139
BfuAI	ACCTGC	1 cut(s)	3073
BfuI	GTATCC	3 cut(s)	1613, 2166, 3142
BmcAI	AGTACT	1 cut(s)	1404
Bme18I	GGWCC	1 cut(s)	3017
BmeRI	GACNNNNNGTC	1 cut(s)	709
BmeT110I	CYCGRG	2 cut(s)	1083, 3998
BmgBI	CACGTC	2 cut(s)	82, 2189
BmgT120I	GGNCC	3 cut(s)	108, 3017, 3495
BmiI	GGNNCC	3 cut(s)	509, 3442, 4164
BmrI	ACTGGG	4 cut(s)	147, 164, 365, 1525
BmuI	ACTGGG	4 cut(s)	147, 164, 365, 1525
BpiI	GAAGAC	2 cut(s)	2673, 2928
BpmI	CTGGAG	3 cut(s)	716, 1134, 1194
Bpu10I	CCTNAGC	2 cut(s)	345, 3516
Bpu14I	TTCGAA	1 cut(s)	3452
BpuEI	CTTGAG	1 cut(s)	2673
BpuMI	CCSGG	3 cut(s)	226, 858, 3866
BsaHI	GRCGYC	2 cut(s)	279, 1155
BsaWI	WCCGGW	1 cut(s)	3293
BsaXI	ACNNNNNCTCC	6 cut(s)	2850, 2880, 3114, 3144, 3974, 4004
Bse118I	RCCGGY	1 cut(s)	215
Bse3DI	GCAATG	7 cut(s)	931, 996, 1041, 2225, 3671, 4098, 4109
BseAI	TCCGGA	1 cut(s)	3293
BseGI	GGATG	9 cut(s)	369, 1039, 1049, 1498, 1899, 2278, 2533, 3076, 3843
BseMI	GCAATG	7 cut(s)	931, 996, 1041, 2225, 3671, 4098, 4109
BseMII	CTCAG	5 cut(s)	336, 2061, 2965, 3507, 3638
BseRI	GAGGAG	3 cut(s)	103, 138, 3521
BsgI	GTGCAG	2 cut(s)	744, 3063
BshFI	GGCC	8 cut(s)	109, 215, 259, 1252, 1569, 1659, 3497, 4144
BsiHKAI	GWGCWC	5 cut(s)	236, 1026, 1372, 2493, 2952
BsiHKCI	CYCGRG	2 cut(s)	1083, 3998
BsiSI	CCGG	5 cut(s)	216, 226, 857, 3294, 3865
BslFI	GGGAC	5 cut(s)	980, 1362, 1496, 2261, 3038
BsmAI	GTCTC	7 cut(s)	270, 1080, 1162, 2066, 3003, 3291, 4014
BsmBI	CGTCTC	2 cut(s)	270, 1162
BsmFI	GGGAC	5 cut(s)	980, 1362, 1496, 2261, 3038
BsmI	GAATGC	1 cut(s)	2182
BsnI	GGCC	8 cut(s)	109, 215, 259, 1252, 1569, 1659, 3497, 4144
BsoBI	CYCGRG	2 cut(s)	1083, 3998
Bsp119I	TTCGAA	1 cut(s)	3452
Bsp1286I	GDGCHC	6 cut(s)	236, 1026, 1372, 2493, 2952, 4182
Bsp13I	TCCGGA	1 cut(s)	3293
Bsp19I	CCATGG	3 cut(s)	1240, 2647, 3690
BspACI	CCGC	7 cut(s)	62, 65, 263, 3213, 3617, 3716, 4145
BspANI	GGCC	8 cut(s)	109, 215, 259, 1252, 1569, 1659, 3497, 4144
BspCNI	CTCAG	5 cut(s)	337, 2062, 2964, 3508, 3637
BspEI	TCCGGA	1 cut(s)	3293
BspHI	TCATGA	1 cut(s)	2335
BspLI	GGNNCC	3 cut(s)	509, 3442, 4164
BspMAI	CTGCAG	4 cut(s)	481, 1945, 3973, 4015
BspMI	ACCTGC	1 cut(s)	3073
BspPI	GGATC	6 cut(s)	187, 868, 1424, 1909, 2908, 3363
BspQI	GCTCTTC	1 cut(s)	3226
BspT104I	TTCGAA	1 cut(s)	3452
BsrDI	GCAATG	7 cut(s)	931, 996, 1041, 2225, 3671, 4098, 4109
BsrFI	RCCGGY	1 cut(s)	215
BssAI	RCCGGY	1 cut(s)	215
BssNI	GRCGYC	2 cut(s)	279, 1155
BssT1I	CCWWGG	8 cut(s)	1240, 1507, 1889, 2442, 2647, 3690, 3852, 4139
Bst4CI	ACNGT	7 cut(s)	791, 877, 1826, 2032, 2065, 2388, 3244
Bst6I	CTCTTC	1 cut(s)	3226
BstACI	GRCGYC	2 cut(s)	279, 1155
BstAPI	GCANNNNNTGC	1 cut(s)	2224
BstBI	TTCGAA	1 cut(s)	3452
BstC8I	GCNNGC	6 cut(s)	217, 649, 1254, 3325, 4015, 4104
BstDEI	CTNAG	6 cut(s)	345, 2070, 2753, 2951, 3516, 3624
BstDSI	CCRYGG	4 cut(s)	1240, 2647, 2957, 3690
BstEII	GGTNACC	1 cut(s)	1802
BstENI	CCTNNNNNAGG	2 cut(s)	264, 2242
BstF5I	GGATG	9 cut(s)	369, 1039, 1049, 1498, 1899, 2278, 2533, 3076, 3843
BstH2I	RGCGCY	1 cut(s)	4139
BstHHI	GCGC	3 cut(s)	4032, 4102, 4138
BstMAI	GTCTC	7 cut(s)	270, 1080, 1162, 2066, 3003, 3291, 4014
BstMWI	GCNNNNNNNGC	9 cut(s)	350, 767, 848, 1345, 2224, 4097, 4144, 4150, 4153
BstNSI	RCATGY	4 cut(s)	723, 1849, 2315, 4054
BstPI	GGTNACC	1 cut(s)	1802
BstSFI	CTRYAG	5 cut(s)	477, 1941, 3609, 3969, 4011
BstV2I	GAAGAC	2 cut(s)	2673, 2928
BstX2I	RGATCY	1 cut(s)	1901
BstXI	CCANNNNNNTGG	3 cut(s)	603, 1538, 2105
BstYI	RGATCY	1 cut(s)	1901
BsuI	GTATCC	3 cut(s)	1613, 2166, 3142
BsuRI	GGCC	8 cut(s)	109, 215, 259, 1252, 1569, 1659, 3497, 4144
BtgI	CCRYGG	4 cut(s)	1240, 2647, 2957, 3690
BtrI	CACGTC	2 cut(s)	82, 2189
BtsCI	GGATG	9 cut(s)	369, 1039, 1049, 1498, 1899, 2278, 2533, 3076, 3843
BtsI	GCAGTG	1 cut(s)	1199
BtsIMutI	CAGTG	4 cut(s)	177, 796, 1199, 2028
BveI	ACCTGC	1 cut(s)	3073
Cac8I	GCNNGC	6 cut(s)	217, 649, 1254, 3325, 4015, 4104
CaiI	CAGNNNCTG	5 cut(s)	1917, 3050, 3533, 4010, 4159
CciI	TCATGA	1 cut(s)	2335
CfoI	GCGC	3 cut(s)	4032, 4102, 4138
Cfr10I	RCCGGY	1 cut(s)	215
Cfr13I	GGNCC	3 cut(s)	108, 3017, 3495
CseI	GACGC	2 cut(s)	2330, 4097
CsiI	ACCWGGT	1 cut(s)	735
Csp6I	GTAC	7 cut(s)	367, 443, 1403, 2430, 3207, 3819, 3888
CspCI	CAANNNNNGTGG	8 cut(s)	1627, 1662, 1878, 1913, 2995, 3030, 3100, 3135
CviQI	GTAC	7 cut(s)	367, 443, 1403, 2430, 3207, 3819, 3888
DdeI	CTNAG	6 cut(s)	345, 2070, 2753, 2951, 3516, 3624
DraIII	CACNNNGTG	1 cut(s)	1367
DrdI	GACNNNNNNGTC	1 cut(s)	941
DriI	GACNNNNNGTC	1 cut(s)	709
DseDI	GACNNNNNNGTC	1 cut(s)	941
Eam1104I	CTCTTC	1 cut(s)	3226
Eam1105I	GACNNNNNGTC	1 cut(s)	709
EarI	CTCTTC	1 cut(s)	3226
EciI	GGCGGA	1 cut(s)	252
Ecl136II	GAGCTC	2 cut(s)	1024, 2950
Eco130I	CCWWGG	8 cut(s)	1240, 1507, 1889, 2442, 2647, 3690, 3852, 4139
Eco147I	AGGCCT	2 cut(s)	259, 1659
Eco24I	GRGCYC	3 cut(s)	1026, 2952, 4182
Eco32I	GATATC	1 cut(s)	3965
Eco47I	GGWCC	1 cut(s)	3017
Eco53kI	GAGCTC	2 cut(s)	1024, 2950
Eco57I	CTGAAG	3 cut(s)	105, 2643, 3510
Eco88I	CYCGRG	2 cut(s)	1083, 3998
Eco91I	GGTNACC	1 cut(s)	1802
EcoICRI	GAGCTC	2 cut(s)	1024, 2950
EcoNI	CCTNNNNNAGG	2 cut(s)	264, 2242
EcoO109I	RGGNCCY	2 cut(s)	108, 3495
EcoO65I	GGTNACC	1 cut(s)	1802
EcoRI	GAATTC	3 cut(s)	2276, 2746, 3475
EcoRV	GATATC	1 cut(s)	3965
EcoT14I	CCWWGG	8 cut(s)	1240, 1507, 1889, 2442, 2647, 3690, 3852, 4139
EcoT22I	ATGCAT	3 cut(s)	1041, 1498, 2184
EcoT38I	GRGCYC	3 cut(s)	1026, 2952, 4182
ErhI	CCWWGG	8 cut(s)	1240, 1507, 1889, 2442, 2647, 3690, 3852, 4139
Esp3I	CGTCTC	2 cut(s)	270, 1162
FaqI	GGGAC	5 cut(s)	980, 1362, 1496, 2261, 3038
FauI	CCCGC	1 cut(s)	3709
FauNDI	CATATG	2 cut(s)	952, 1258
FbaI	TGATCA	2 cut(s)	1411, 3559
FblI	GTMKAC	1 cut(s)	3876
FokI	GGATG	9 cut(s)	376, 1026, 1036, 1505, 1906, 2285, 2540, 3063, 3850
FriOI	GRGCYC	3 cut(s)	1026, 2952, 4182
FspBI	CTAG	5 cut(s)	1775, 1884, 2700, 2760, 3012
GlaI	GCGC	3 cut(s)	4031, 4101, 4137
GsuI	CTGGAG	3 cut(s)	716, 1134, 1194
HaeII	RGCGCY	1 cut(s)	4139
HaeIII	GGCC	8 cut(s)	109, 215, 259, 1252, 1569, 1659, 3497, 4144
HapII	CCGG	5 cut(s)	216, 226, 857, 3294, 3865
HgaI	GACGC	2 cut(s)	2330, 4097
HhaI	GCGC	3 cut(s)	4032, 4102, 4138
Hin1I	GRCGYC	2 cut(s)	279, 1155
Hin6I	GCGC	3 cut(s)	4030, 4100, 4136
HinP1I	GCGC	3 cut(s)	4030, 4100, 4136
HincII	GTYRAC	4 cut(s)	2061, 3405, 3831, 3976
HindII	GTYRAC	4 cut(s)	2061, 3405, 3831, 3976
HpaI	GTTAAC	1 cut(s)	2061
HpaII	CCGG	5 cut(s)	216, 226, 857, 3294, 3865
Hpy99I	CGWCG	7 cut(s)	35, 38, 41, 44, 83, 284, 2193
HpyCH4III	ACNGT	7 cut(s)	791, 877, 1826, 2032, 2065, 2388, 3244
HpyCH4IV	ACGT	9 cut(s)	81, 279, 969, 1155, 1704, 2057, 2153, 2188, 3667
HpyF10VI	GCNNNNNNNGC	9 cut(s)	350, 767, 848, 1345, 2224, 4097, 4144, 4150, 4153
HpyF3I	CTNAG	6 cut(s)	345, 2070, 2753, 2951, 3516, 3624
HpySE526I	ACGT	9 cut(s)	81, 279, 969, 1155, 1704, 2057, 2153, 2188, 3667
Hsp92I	GRCGYC	2 cut(s)	279, 1155
HspAI	GCGC	3 cut(s)	4030, 4100, 4136
Kpn2I	TCCGGA	1 cut(s)	3293
KroI	GCCGGC	1 cut(s)	215
KroNI	GCCGGC	1 cut(s)	217
Ksp22I	TGATCA	2 cut(s)	1411, 3559
KspAI	GTTAAC	1 cut(s)	2061
LguI	GCTCTTC	1 cut(s)	3226
LmnI	GCTCC	4 cut(s)	541, 1375, 3982, 4150
MabI	ACCWGGT	1 cut(s)	735
MaeI	CTAG	5 cut(s)	1775, 1884, 2700, 2760, 3012
MaeII	ACGT	9 cut(s)	81, 279, 969, 1155, 1704, 2057, 2153, 2188, 3667
MaeIII	GTNAC	7 cut(s)	155, 679, 1359, 1572, 1802, 2583, 3385
MfeI	CAATTG	2 cut(s)	336, 609
MflI	RGATCY	1 cut(s)	1901
MhlI	GDGCHC	6 cut(s)	236, 1026, 1372, 2493, 2952, 4182
MlyI	GAGTC	5 cut(s)	33, 1384, 2032, 3005, 4010
MmeI	TCCRAC	6 cut(s)	2171, 2370, 2601, 2895, 3285, 3341
Mph1103I	ATGCAT	3 cut(s)	1041, 1498, 2184
MroI	TCCGGA	1 cut(s)	3293
MroNI	GCCGGC	1 cut(s)	215
MroXI	GAANNNNTTC	4 cut(s)	781, 2656, 2795, 2814
MslI	CAYNNNNRTG	3 cut(s)	170, 601, 2103
MspA1I	CMGCKG	1 cut(s)	3614
MspI	CCGG	5 cut(s)	216, 226, 857, 3294, 3865
MunI	CAATTG	2 cut(s)	336, 609
Mva1269I	GAATGC	1 cut(s)	2182
MwoI	GCNNNNNNNGC	9 cut(s)	350, 767, 848, 1345, 2224, 4097, 4144, 4150, 4153
NaeI	GCCGGC	1 cut(s)	217
NciI	CCSGG	3 cut(s)	226, 858, 3866
NcoI	CCATGG	3 cut(s)	1240, 2647, 3690
NdeI	CATATG	2 cut(s)	952, 1258
NgoMIV	GCCGGC	1 cut(s)	215
NlaIV	GGNNCC	3 cut(s)	509, 3442, 4164
NmeAIII	GCCGAG	1 cut(s)	364
NmuCI	GTSAC	4 cut(s)	1359, 1572, 2583, 3385
NsiI	ATGCAT	3 cut(s)	1041, 1498, 2184
NspI	RCATGY	4 cut(s)	723, 1849, 2315, 4054
NspV	TTCGAA	1 cut(s)	3452
OliI	CACNNNNGTG	1 cut(s)	170
PaeR7I	CTCGAG	2 cut(s)	1083, 3998
PagI	TCATGA	1 cut(s)	2335
PaqCI	CACCTGC	1 cut(s)	3073
PceI	AGGCCT	2 cut(s)	259, 1659
PciI	ACATGT	3 cut(s)	719, 2311, 4050
PciSI	GCTCTTC	1 cut(s)	3226
PcsI	WCGNNNNNNNCGW	2 cut(s)	36, 39
PctI	GAATGC	1 cut(s)	2182
PdiI	GCCGGC	1 cut(s)	217
PdmI	GAANNNNTTC	4 cut(s)	781, 2656, 2795, 2814
PfeI	GAWTC	9 cut(s)	271, 979, 1723, 1986, 2362, 2372, 2408, 3731, 3842
PflMI	CCANNNNNTGG	1 cut(s)	3227
PleI	GAGTC	5 cut(s)	32, 1383, 2032, 3004, 4009
PpsI	GAGTC	5 cut(s)	32, 1383, 2032, 3004, 4009
PscI	ACATGT	3 cut(s)	719, 2311, 4050
PsiI	TTATAA	2 cut(s)	1451, 1928
Psp124BI	GAGCTC	2 cut(s)	1026, 2952
Psp1406I	AACGTT	1 cut(s)	2057
PspEI	GGTNACC	1 cut(s)	1802
PspN4I	GGNNCC	3 cut(s)	509, 3442, 4164
PspPI	GGNCC	3 cut(s)	108, 3017, 3495
PspXI	VCTCGAGB	1 cut(s)	3998
PsrI	GAACNNNNNNTAC	2 cut(s)	1189, 1221
PstI	CTGCAG	4 cut(s)	481, 1945, 3973, 4015
PstNI	CAGNNNCTG	5 cut(s)	1917, 3050, 3533, 4010, 4159
PsuI	RGATCY	1 cut(s)	1901
PvuII	CAGCTG	1 cut(s)	3614
RsaI	GTAC	7 cut(s)	368, 444, 1404, 2431, 3208, 3820, 3889
RsaNI	GTAC	7 cut(s)	367, 443, 1403, 2430, 3207, 3819, 3888
RseI	CAYNNNNRTG	3 cut(s)	170, 601, 2103
SacI	GAGCTC	2 cut(s)	1026, 2952
SapI	GCTCTTC	1 cut(s)	3226
Sau96I	GGNCC	3 cut(s)	108, 3017, 3495
ScaI	AGTACT	1 cut(s)	1404
SchI	GAGTC	5 cut(s)	33, 1384, 2032, 3005, 4010
SduI	GDGCHC	6 cut(s)	236, 1026, 1372, 2493, 2952, 4182
SexAI	ACCWGGT	1 cut(s)	735
SfcI	CTRYAG	5 cut(s)	477, 1941, 3609, 3969, 4011
Sfr274I	CTCGAG	2 cut(s)	1083, 3998
SfuI	TTCGAA	1 cut(s)	3452
SinI	GGWCC	1 cut(s)	3017
SlaI	CTCGAG	2 cut(s)	1083, 3998
SmiMI	CAYNNNNRTG	3 cut(s)	170, 601, 2103
SmlI	CTYRAG	3 cut(s)	1083, 2688, 3998
SmoI	CTYRAG	3 cut(s)	1083, 2688, 3998
SpeI	ACTAGT	1 cut(s)	3011
SseBI	AGGCCT	2 cut(s)	259, 1659
SsiI	CCGC	7 cut(s)	62, 65, 263, 3213, 3617, 3716, 4145
SspI	AATATT	1 cut(s)	583
SspMI	CTAG	5 cut(s)	1775, 1884, 2700, 2760, 3012
SstI	GAGCTC	2 cut(s)	1026, 2952
StuI	AGGCCT	2 cut(s)	259, 1659
StyI	CCWWGG	8 cut(s)	1240, 1507, 1889, 2442, 2647, 3690, 3852, 4139
TaaI	ACNGT	7 cut(s)	791, 877, 1826, 2032, 2065, 2388, 3244
TaiI	ACGT	9 cut(s)	84, 282, 972, 1158, 1707, 2060, 2156, 2191, 3670
TatI	WGTACW	3 cut(s)	1402, 2429, 3887
TauI	GCSGC	4 cut(s)	65, 68, 3620, 4147
TfiI	GAWTC	9 cut(s)	271, 979, 1723, 1986, 2362, 2372, 2408, 3731, 3842
TscAI	CASTG	4 cut(s)	177, 796, 1206, 2035
TseFI	GTSAC	4 cut(s)	1359, 1572, 2583, 3385
Tsp45I	GTSAC	4 cut(s)	1359, 1572, 2583, 3385
TspGWI	ACGGA	1 cut(s)	2148
TspRI	CASTG	4 cut(s)	177, 796, 1206, 2035
Van91I	CCANNNNNTGG	1 cut(s)	3227
VpaK11BI	GGWCC	1 cut(s)	3017
XagI	CCTNNNNNAGG	2 cut(s)	264, 2242
XbaI	TCTAGA	1 cut(s)	2759
XceI	RCATGY	4 cut(s)	723, 1849, 2315, 4054
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1169
XhoI	CTCGAG	2 cut(s)	1083, 3998
XmiI	GTMKAC	1 cut(s)	3876
XmnI	GAANNNNTTC	4 cut(s)	781, 2656, 2795, 2814
XspI	CTAG	5 cut(s)	1775, 1884, 2700, 2760, 3012
ZraI	GACGTC	2 cut(s)	280, 1156
ZrmI	AGTACT	1 cut(s)	1404
Zsp2I	ATGCAT	3 cut(s)	1041, 1498, 2184

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.