Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002489

Physical Location & Seq

Genomic Coordinates Forward (+)

17209220 .. 17221154

Gene Structure 11935 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001539

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4125 bp

ATGACCTCCACCGAACCTCACCACATAGTCGACGACGACGACGAAGACGACGATTTCGATTGGGAAGCAGCTGCCAGAGCCGTCGACGTCGTCACCCAGACCACCGCAAAGCCCTCATCTTCCCATTTTGCCCCTCCTCCCCCAATTCATAAACCACCATTTCCTCCAATCTTCCACAACAAGCCCGGGACTTCACGGCAGACCACTCTCGATAGGTTCATTGGGAAGCCCGCCCCAAAGCCTCAGCCCGAAATTCGAGACTGCGGCGATGAGAGAGTGTCCTGTGTTCAAATTGATGCCGAAAAAGCCAACACTTGGATGTACCCAGTTAATTTTCCTGGGCGTGACTATCAATTTGCTATCACCAAGACAGCACTTTTTTCGAATACGCTGGTGGCATTGCCAACCGGACTCGGGAAGACACTTATCGCTGCAGTTGTTATGTATAACTACTTCAGATGGTTTCCTGATGGAAAGATAGTCTTTGCTGCTCCTTCTCGACCACTTGTTACACAACAAATAAAAGCATGCCATAATATTGTAGGAATACCACAAGAATGGACAATTGATATGACAGGTCAGATAAGTCCTACAAAAAGAGCATGCTTTTGGAAAACTAAACGAGTTTTCTTTGTTACTCCACAAGTGCTGGAGAAGGACATTCAGTCTGGAACATGCTCAGTCGAATATTTGGTATGCTTGGTGATTGATGAAGCTCACCGAGCATTAGGGAACTATGCTTCTAGTGTTGCAGTTCGTGAGTTAATGGCGGCACAAGTGCCAGTGAGAATTTTAGCTTTGACTGCAACACCAGGATCTAAGCAGCAGACTGTTCAGCAAATTATTGATAACTTGTGTATATCATCACTTGAATACCGTGAAGAAAATGATGCTGATGTCAGCCCTTATGTTCACAACAGGGAGGTAGAGTTGATTAAGGTGGCAATGGGTCAAGATGCTGTGGAGATAAGTAATAAGATATATGATGCAATACGACCATATTTAACCAGGCTTAGTGCGGCTGGAGTTCTCCCAAATAGAGACGCTCAAACGTGGAGCCCAGGCGAGTTACTTAGGTTTAGGGAGAAATTTCGTCTTGCACCTCCTCCAGACTTCCCCCAATTTAAATATGGAGAAATTGAAGGTTGGTATGGAGTTCTTCTTACAGGTTACCACATTCTTAAGCTATTATCAAGCCATGGAATAAGACCAGCATATGACATGCTTGAAGAAAAGTTAAAAAAGGGGTTTTTTGCCAAGTATATGAGTAGAAGTGAAGATGTTCATAAAGCAAAGCTAAGGATGCAGCAAAGTCTGTCTCATGGTGGTCCGAGTCCCAAGTTATCCAAAATGTTAGAAGTATTGCTTGATCATTTCAAAACAAAAGATCCACAAAACTCGAGGGTTATGATTTTCTCAAACTTCAGGGGAAGTGTCAGGGAGATAATGGATGCATTGGCAAACATTGGGAATTTAGTTAGAGCTACCGAGTTTATTGGTCAGAGTTCAGGGAAAATATTAAAGGGCCAGTCACAAAAAGTTCAACAAGCTGTGTTGGAGAAATTTCGTGCTGGTGGATACAATGTCATTGTTGCCACATCAATTGGCGAAGAAGGTTTGGATATAATGGAAGTTGATCTTGTTATATGCTTTGATGCAAATGTATCACCATTGAGGATGATTCAACGCATGGGGAGAACTGGAAGGAAGCATGATGGACGATTGAAAGGTTACAAGCGGAAGCTAACAAACAGCAAGAATGTGAGCAAACACATGCGAAGTGGGGGAATAAATAGCTTCAATTTTCATTCTAGCCCGAGGATGATCCCTCACATTTTCAAACCTGAAGTCCAATATGTTGAGTTTTCAATTGAACAATTCGTTTGTCCTGGAAAGAAAGTCAAAGATGATAATGATATTCAGACACCTGTTACTATAGACAAATTAACTGTTGCTGAGACTAGTTTAGTTGCCAAGTATTTCCAGTCACATGACGTAACATGGAAACCATCTCTTATTGCCTTTCCTCACTTCCAAACCTATCCCTCAAGAGTGTATAAAGTAATGCACTCGCCTCGAACAACAATGCTAATTGATACAATGCAATCTCTGCAAGGATTATCCTTTTCCAGTGACAGAAAAATTTCCCTCATCGAGGATGAGGTCTGTTCAGAGGATTGCTTAGCAGTTGACACTGTCGAACAGCGTGACAACAGAGAAGATTTGCTGAATCTTGATGGTTCAAAGAAACAATCACAGGAAATAGTACTGGATTTCGAACTACAAACTACTGGGACATTGGAAACCAAGGAGAAGCATAGTGACCATGATTTATCTGATGAAAATCCTTCTGGTCACTCTTTTCTCTTTGGTTCAGATGTTGTTGATGTTGATGCTTCTGGAAAGGTCCTTATCATATCTGTCCCTATTCTTCATGGCAAAAGTATACCAAATTCCAAGTTTACAAGTGCAAGCAGTGGTAAGTTGGATCATTTGAAGCAAAGTTCTTGCCATGTAAGAACATCAGATGAAGAACAAACAGAAGTAACTACTCGAGCTGGAGCTAATGGGGATCCTGAAATGGCTCAGATAGCATATATAAGGAATGATACACCGCCTTGGTCTAGATTGTGCTACTCTGAAACCTGGATGGAGACAACAATCAATGGGGATGAAGAGAATCCTCAAAAGCCAATTCTCAAAGGAAACTTTCCAAATGGAGATAGTGCTTGTGAGACGCCTGATGTTCTGAAAAGCAAGGCACCGACATTGCTGGCTGATGAAGACGACAACCTTTTCATAGATGGTGAGCTTAGTCCAAGGCTCACTAATTTGATCAAAAGTGGAGTAGTTCCAGAGTCACCTAACAATAATAGTGGAATATTAGATACCATAAATAAGTGTGTAGTTCCTGATGCTGTTTCGCCTGCCCCATTACCTACGGAACTGATAACGAAGTCTTCAAGTCCTGGGAAAAATGGAAAAGCCACTGATATGGATACCAGTGCATGTGAAAGAAATGTGTCTCTTTTTCCTACTGATATTGAAATTCAATCTCCCTTGCACAATAGGAGTACTGCCAGTATAAATGAATCTACCTCCACATCACCGGTTATTGACAGAGGCCAAACTGTTTTAGCAGACCTTACAAACAGTAGCTGTAGCAAAGATTGGTGTATAGGTTCTGGAGACAAGTCGCAGAGTGTGAAACAGACACGCAAGTTTAAAAGGTTGCGCAAATTTGGGGATCATTGGAAAAGTAGGAATCAGGAAAGCATGACAACCAATGATGTCCCAAGAGCAAACCTGACCAGATCTTTTACAAGTACTACTCTTCCTACTAAGCATGTTAGGGGTACCAAAATACGTGCAAAGAAGTCGGTTAATGATGTGAGGGAATTCATCGACGAGGAAGCTGAGGTATCTTCAGAGGATATATCTGATAATGAAGAAGATGAACCAGACAACTATTCAAATGATAGTTTCATAGATGACAGGATAAATTCAACGGCTGCAAGTACTCAGGCTGCGACCAGTGGAATTGACATGATGGCAGTGTACAGGCGTTCATTGCTCACCCAATCACCCATGGAGAAGCAGCTAAGCAGTTCAGCCACATTTTCTCCCAATTCTGTGGCAACGATAACTAGGACACCGGAAACTGGAAGTTCTGGTGGAAAAACATCTTTCTCTCTTCAGACTCCTCAAAGCGACTCTACAAATCTGCCAATTGGAATAGATTCAGAGTCTTTCCATAAGAATTGGAACTCTGAAGCCAGAACTTTTACAACTGGTGTTTCTCCAGGATATGAAAGAGATATTGAAAGTCGAAAAAGAAAATCAAGTTTTCATCATTCAAGATCTATTCCTGCTGTCAACTTGGAGCTAGAATTCTCGTTGCAATCAGAGGCTGCAGGCAGAAATATGCAGTACAGTGATGCGCATGGGGACATGCTTTATGATGATCAGTTTTATGAGGATCTTGATCTTGACGCATTGGAAGCACAAGCTACAATTCTTCTTAAGCAGAGATCAGAATTGCCAGTGCAGAAGCCACAGATGAGTCAGTTAAACCCAGAAAATCCTACTCTTGACAGTGCTCCATCATTTGATCTTGGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0031297 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043240 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045005 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071704 GO:0071821 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

Helicase_C ResIII

Protein Analysis

1375

Amino Acids

152.88

Weight (kDa)

5.77

Isoelectric Point (pI)

49.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	121 - 275	2.2e-17	DEAD/DEAH box helicase
ResIII	PF04851	127 - 271	1.2e-17	Type III restriction enzyme, res subunit
Helicase_C	PF00271	454 - 570	1.3e-19	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G36020
fragaria_vesca	FvH4_7g03750
malus_domestica	MD15G1404400.v1.1
prunus_persica	Prupe.1G551100_v2.0.a1 Prupe.2G019600_v2.0.a1 Prupe.2G019700_v2.0.a1
pyrus_communis	pycom15g36170 pycom15g36180
rosa_chinensis	RchiOBHm_Chr7g0228871 RchiOBHm_Chr7g0228881 RchiOBHm_Chr7g0228951
rosa_laevigata	RLG00000001539 RLG00000002150 RLG00000022432
rosa_multiflora	Rmu_sc0000721.1_g000006 Rmu_sc0004517.1_g000001 Rmu_sc0005913.1_g000001 Rmu_sc0005913.1_g000026 Rmu_sc0011218.1_g000006 Rmu_sc0013945.1_g000010
rosa_roxburghii	Rroxscaffold_3G00230830 Rroxscaffold_3G00238230
rosa_rugosa	Rorug07G0252600 Rorug07G0254200
rosa_samantha	Rh7AG405800 Rh7BG333000 Rh7BG385100 Rh7BG385200 Rh7BG385300 Rh7CG423400 Rh7DG341000 Rh7DG399800 Rh7DG399900
rosa_wichuraiana	Rw7G033620 Rw7G033650 Rw7G033670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	90
Acc16I	TGCGCA	2 cut(s)	3236, 3942
Acc65I	GGTACC	1 cut(s)	3356
AccB1I	GGYRCC	2 cut(s)	2761, 3356
AccB7I	CCANNNNNTGG	1 cut(s)	315
AccI	GTMKAC	3 cut(s)	30, 84, 2446
AciI	CCGC	7 cut(s)	105, 231, 264, 770, 1019, 1738, 2615
AclWI	GGATC	8 cut(s)	823, 1382, 1819, 2496, 2567, 2580, 3255, 3987
AcuI	CTGAAG	6 cut(s)	439, 1408, 1866, 3411, 3680, 3792
AcyI	GRCGYC	2 cut(s)	87, 2738
AfaI	GTAC	8 cut(s)	323, 2268, 3076, 3328, 3358, 3520, 3560, 3932
AfiI	CCNNNNNNNGG	4 cut(s)	315, 414, 2155, 3662
AflII	CTTAAG	2 cut(s)	1181, 4022
AgeI	ACCGGT	1 cut(s)	3109
AhdI	GACNNNNNGTC	1 cut(s)	664
AhlI	ACTAGT	1 cut(s)	1961
AjnI	CCWGG	8 cut(s)	337, 811, 1007, 1060, 1888, 2645, 2968, 3802
Alw21I	GWGCWC	1 cut(s)	4102
Alw26I	GTCTC	8 cut(s)	252, 1035, 1323, 1952, 2648, 2729, 3030, 3183
AlwI	GGATC	8 cut(s)	823, 1382, 1819, 2496, 2567, 2580, 3255, 3987
AlwNI	CAGNNNCTG	2 cut(s)	3159, 3911
Ama87I	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2553
AoxI	GGCC	2 cut(s)	1525, 3124
ArsI	GACNNNNNNTTYG	6 cut(s)	38, 70, 338, 370, 1833, 1865
AsiGI	ACCGGT	1 cut(s)	3109
Asp700I	GAANNNNTTC	3 cut(s)	1281, 2143, 3739
Asp718I	GGTACC	1 cut(s)	3356
AspLEI	GCGC	2 cut(s)	3237, 3943
AspS9I	GGNCC	3 cut(s)	1328, 1525, 2407
AsuC2I	CCSGG	2 cut(s)	186, 187
AsuII	TTCGAA	2 cut(s)	383, 2277
AvaI	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2553
AvaII	GGWCC	2 cut(s)	1328, 2407
BamHI	GGATCC	1 cut(s)	2572
BanI	GGYRCC	2 cut(s)	2761, 3356
BanII	GRGCYC	1 cut(s)	1061
BarI	GAAGNNNNNNTAC	2 cut(s)	437, 469
BbsI	GAAGAC	4 cut(s)	51, 425, 2790, 2952
Bbv12I	GWGCWC	1 cut(s)	4102
BbvCI	CCTCAGC	2 cut(s)	243, 3417
BccI	CCATC	9 cut(s)	453, 464, 1709, 2017, 2231, 2644, 2798, 3544, 4111
BceAI	ACGGC	3 cut(s)	65, 212, 3525
BcgI	CGANNNNNNTGC	6 cut(s)	363, 397, 2057, 2091, 3360, 3394
BciT130I	CCWGG	8 cut(s)	339, 813, 1009, 1062, 1890, 2647, 2970, 3804
BciVI	GTATCC	2 cut(s)	1571, 2992
BclI	TGATCA	3 cut(s)	1369, 2835, 3964
BcnI	CCSGG	2 cut(s)	186, 187
BcoDI	GTCTC	8 cut(s)	252, 1035, 1323, 1952, 2648, 2729, 3030, 3183
BcuI	ACTAGT	1 cut(s)	1961
BfaI	CTAG	6 cut(s)	744, 1812, 1962, 2625, 3648, 3887
BfmI	CTRYAG	4 cut(s)	432, 1935, 3160, 3912
BfrI	CTTAAG	2 cut(s)	1181, 4022
BfuI	GTATCC	2 cut(s)	1571, 2992
BglII	AGATCT	2 cut(s)	3314, 3860
BlpI	GCTNAGC	2 cut(s)	2182, 3602
BmcAI	AGTACT	4 cut(s)	2268, 3076, 3328, 3520
Bme18I	GGWCC	2 cut(s)	1328, 2407
BmeRI	GACNNNNNGTC	1 cut(s)	664
BmeT110I	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2553
BmgT120I	GGNCC	3 cut(s)	1328, 1525, 2407
BmiI	GGNNCC	4 cut(s)	1058, 2574, 2763, 3358
BmrI	ACTGGG	2 cut(s)	320, 2301
BmuI	ACTGGG	2 cut(s)	320, 2301
BpiI	GAAGAC	4 cut(s)	51, 425, 2790, 2952
BpmI	CTGGAG	6 cut(s)	671, 1044, 1092, 2580, 3207, 3786
Bpu10I	CCTNAGC	3 cut(s)	243, 1298, 3417
Bpu1102I	GCTNAGC	2 cut(s)	2182, 3602
Bpu14I	TTCGAA	2 cut(s)	383, 2277
BpuEI	CTTGAG	1 cut(s)	2032
BpuMI	CCSGG	2 cut(s)	186, 187
BsaAI	YACGTR	1 cut(s)	3368
BsaBI	GATNNNNATC	2 cut(s)	2343, 3984
BsaHI	GRCGYC	2 cut(s)	87, 2738
BsaWI	WCCGGW	3 cut(s)	407, 3109, 3655
BsaXI	ACNNNNNCTCC	4 cut(s)	914, 944, 3607, 3637
Bsc4I	CCNNNNNNNGG	4 cut(s)	315, 414, 2155, 3662
Bse118I	RCCGGY	1 cut(s)	3109
Bse3DI	GCAATG	4 cut(s)	398, 951, 2768, 3569
Bse8I	GATNNNNATC	2 cut(s)	2343, 3984
BseBI	CCWGG	8 cut(s)	339, 813, 1009, 1062, 1890, 2647, 2970, 3804
BseGI	GGATG	8 cut(s)	324, 1308, 1456, 1683, 1827, 2164, 2655, 2677
BseJI	GATNNNNATC	2 cut(s)	2343, 3984
BseLI	CCNNNNNNNGG	4 cut(s)	315, 414, 2155, 3662
BseMI	GCAATG	4 cut(s)	398, 951, 2768, 3569
BseMII	CTCAG	6 cut(s)	257, 693, 1947, 2600, 3408, 3536
BseRI	GAGGAG	3 cut(s)	126, 1095, 3693
BsgI	GTGCAG	1 cut(s)	4067
BshFI	GGCC	2 cut(s)	1527, 3126
BshNI	GGYRCC	2 cut(s)	2761, 3356
BshTI	ACCGGT	1 cut(s)	3109
BsiHKAI	GWGCWC	1 cut(s)	4102
BsiHKCI	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2553
BsiSI	CCGG	4 cut(s)	186, 408, 3110, 3656
BslFI	GGGAC	6 cut(s)	202, 1320, 2308, 2408, 3278, 3962
BslI	CCNNNNNNNGG	4 cut(s)	315, 414, 2155, 3662
BsmAI	GTCTC	8 cut(s)	252, 1035, 1323, 1952, 2648, 2729, 3030, 3183
BsmBI	CGTCTC	2 cut(s)	1035, 2729
BsmFI	GGGAC	6 cut(s)	202, 1320, 2308, 2408, 3278, 3962
BsnI	GGCC	2 cut(s)	1527, 3126
BsoBI	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2553
Bsp119I	TTCGAA	2 cut(s)	383, 2277
Bsp1286I	GDGCHC	2 cut(s)	1061, 4102
Bsp1407I	TGTACA	1 cut(s)	3558
Bsp1720I	GCTNAGC	2 cut(s)	2182, 3602
Bsp19I	CCATGG	2 cut(s)	1198, 3588
BspACI	CCGC	7 cut(s)	105, 231, 264, 770, 1019, 1738, 2615
BspANI	GGCC	2 cut(s)	1527, 3126
BspCNI	CTCAG	6 cut(s)	256, 692, 1948, 2599, 3409, 3535
BspLI	GGNNCC	4 cut(s)	1058, 2574, 2763, 3358
BspMAI	CTGCAG	2 cut(s)	436, 3916
BspPI	GGATC	8 cut(s)	823, 1382, 1819, 2496, 2567, 2580, 3255, 3987
BspT104I	TTCGAA	2 cut(s)	383, 2277
BspT107I	GGYRCC	2 cut(s)	2761, 3356
BspTI	CTTAAG	2 cut(s)	1181, 4022
BsrDI	GCAATG	4 cut(s)	398, 951, 2768, 3569
BsrFI	RCCGGY	1 cut(s)	3109
BsrGI	TGTACA	1 cut(s)	3558
BssAI	RCCGGY	1 cut(s)	3109
BssNAI	GTATAC	1 cut(s)	2447
BssNI	GRCGYC	2 cut(s)	87, 2738
BssT1I	CCWWGG	5 cut(s)	1198, 2307, 2618, 2819, 3588
Bst1107I	GTATAC	1 cut(s)	2447
Bst2UI	CCWGG	8 cut(s)	339, 813, 1009, 1062, 1890, 2647, 2970, 3804
Bst4CI	ACNGT	8 cut(s)	832, 878, 1951, 2197, 3133, 3155, 3935, 4097
Bst6I	CTCTTC	3 cut(s)	2670, 3339, 3699
BstACI	GRCGYC	2 cut(s)	87, 2738
BstAFI	CTTAAG	2 cut(s)	1181, 4022
BstAPI	GCANNNNNTGC	1 cut(s)	2110
BstAUI	TGTACA	1 cut(s)	3558
BstBAI	YACGTR	1 cut(s)	3368
BstBI	TTCGAA	2 cut(s)	383, 2277
BstC8I	GCNNGC	7 cut(s)	231, 529, 604, 2473, 2775, 2928, 3916
BstDSI	CCRYGG	2 cut(s)	1198, 3588
BstEII	GGTNACC	1 cut(s)	1169
BstENI	CCTNNNNNAGG	1 cut(s)	2153
BstF5I	GGATG	8 cut(s)	324, 1308, 1456, 1683, 1827, 2164, 2655, 2677
BstHHI	GCGC	2 cut(s)	3237, 3943
BstMAI	GTCTC	8 cut(s)	252, 1035, 1323, 1952, 2648, 2729, 3030, 3183
BstMWI	GCNNNNNNNGC	9 cut(s)	77, 305, 722, 803, 1303, 2110, 2591, 3571, 4001
BstNI	CCWGG	8 cut(s)	339, 813, 1009, 1062, 1890, 2647, 2970, 3804
BstNSI	RCATGY	8 cut(s)	531, 606, 678, 1225, 1777, 3012, 3350, 3955
BstPI	GGTNACC	1 cut(s)	1169
BstSFI	CTRYAG	4 cut(s)	432, 1935, 3160, 3912
BstV2I	GAAGAC	4 cut(s)	51, 425, 2790, 2952
BstX2I	RGATCY	6 cut(s)	815, 1387, 2572, 3314, 3860, 3979
BstXI	CCANNNNNNTGG	3 cut(s)	558, 2995, 3634
BstYI	RGATCY	6 cut(s)	815, 1387, 2572, 3314, 3860, 3979
BstZ17I	GTATAC	1 cut(s)	2447
BsuI	GTATCC	2 cut(s)	1571, 2992
BsuRI	GGCC	2 cut(s)	1527, 3126
BtgI	CCRYGG	2 cut(s)	1198, 3588
BtgZI	GCGATG	1 cut(s)	282
BtsCI	GGATG	8 cut(s)	324, 1308, 1456, 1683, 1827, 2164, 2655, 2677
BtsI	GCAGTG	2 cut(s)	2482, 3561
Cac8I	GCNNGC	7 cut(s)	231, 529, 604, 2473, 2775, 2928, 3916
CaiI	CAGNNNCTG	2 cut(s)	3159, 3911
CfoI	GCGC	2 cut(s)	3237, 3943
Cfr10I	RCCGGY	1 cut(s)	3109
Cfr13I	GGNCC	3 cut(s)	1328, 1525, 2407
Cfr9I	CCCGGG	1 cut(s)	185
CseI	GACGC	3 cut(s)	1052, 2746, 4001
Csp6I	GTAC	8 cut(s)	322, 2267, 3075, 3327, 3357, 3519, 3559, 3931
CspAI	ACCGGT	1 cut(s)	3109
CspCI	CAANNNNNGTGG	4 cut(s)	1585, 1620, 2857, 2892
CviQI	GTAC	8 cut(s)	322, 2267, 3075, 3327, 3357, 3519, 3559, 3931
DraI	TTTAAA	2 cut(s)	1126, 3226
DriI	GACNNNNNGTC	1 cut(s)	664
Eam1104I	CTCTTC	3 cut(s)	2670, 3339, 3699
Eam1105I	GACNNNNNGTC	1 cut(s)	664
EarI	CTCTTC	3 cut(s)	2670, 3339, 3699
Eco130I	CCWWGG	5 cut(s)	1198, 2307, 2618, 2819, 3588
Eco24I	GRGCYC	1 cut(s)	1061
Eco47I	GGWCC	2 cut(s)	1328, 2407
Eco57I	CTGAAG	6 cut(s)	439, 1408, 1866, 3411, 3680, 3792
Eco88I	CYCGRG	5 cut(s)	185, 413, 1399, 1816, 2553
Eco91I	GGTNACC	1 cut(s)	1169
EcoNI	CCTNNNNNAGG	1 cut(s)	2153
EcoO109I	RGGNCCY	1 cut(s)	2407
EcoO65I	GGTNACC	1 cut(s)	1169
EcoRI	GAATTC	2 cut(s)	3398, 3890
EcoRII	CCWGG	8 cut(s)	337, 811, 1007, 1060, 1888, 2645, 2968, 3802
EcoT14I	CCWWGG	5 cut(s)	1198, 2307, 2618, 2819, 3588
EcoT22I	ATGCAT	1 cut(s)	1456
EcoT38I	GRGCYC	1 cut(s)	1061
ErhI	CCWWGG	5 cut(s)	1198, 2307, 2618, 2819, 3588
Esp3I	CGTCTC	2 cut(s)	1035, 2729
FalI	AAGNNNNNCTT	6 cut(s)	467, 499, 1350, 1382, 1623, 1655
FaqI	GGGAC	6 cut(s)	202, 1320, 2308, 2408, 3278, 3962
FauI	CCCGC	1 cut(s)	238
FauNDI	CATATG	1 cut(s)	1216
FbaI	TGATCA	3 cut(s)	1369, 2835, 3964
FblI	GTMKAC	3 cut(s)	30, 84, 2446
FokI	GGATG	8 cut(s)	331, 1315, 1463, 1690, 1834, 2171, 2662, 2684
FriOI	GRGCYC	1 cut(s)	1061
FspAI	RTGCGCAY	1 cut(s)	3942
FspBI	CTAG	6 cut(s)	744, 1812, 1962, 2625, 3648, 3887
FspI	TGCGCA	2 cut(s)	3236, 3942
GlaI	GCGC	2 cut(s)	3236, 3942
GsuI	CTGGAG	6 cut(s)	671, 1044, 1092, 2580, 3207, 3786
HaeIII	GGCC	2 cut(s)	1527, 3126
HapII	CCGG	4 cut(s)	186, 408, 3110, 3656
HgaI	GACGC	3 cut(s)	1052, 2746, 4001
HhaI	GCGC	2 cut(s)	3237, 3943
Hin1I	GRCGYC	2 cut(s)	87, 2738
Hin6I	GCGC	2 cut(s)	3235, 3941
HinP1I	GCGC	2 cut(s)	3235, 3941
HincII	GTYRAC	4 cut(s)	31, 85, 2191, 3877
HindII	GTYRAC	4 cut(s)	31, 85, 2191, 3877
HpaII	CCGG	4 cut(s)	186, 408, 3110, 3656
Hpy166II	GTNNAC	8 cut(s)	31, 85, 913, 2191, 2447, 2463, 3559, 3877
Hpy8I	GTNNAC	8 cut(s)	31, 85, 913, 2191, 2447, 2463, 3559, 3877
Hpy99I	CGWCG	9 cut(s)	35, 38, 41, 44, 53, 86, 89, 92, 3410
HpyAV	CCTTC	6 cut(s)	504, 649, 1136, 1607, 1698, 2358
HpyCH4III	ACNGT	8 cut(s)	832, 878, 1951, 2197, 3133, 3155, 3935, 4097
HpyCH4IV	ACGT	4 cut(s)	87, 1052, 1995, 3367
HpyF10VI	GCNNNNNNNGC	9 cut(s)	77, 305, 722, 803, 1303, 2110, 2591, 3571, 4001
HpySE526I	ACGT	4 cut(s)	87, 1052, 1995, 3367
Hsp92I	GRCGYC	2 cut(s)	87, 2738
HspAI	GCGC	2 cut(s)	3235, 3941
KpnI	GGTACC	1 cut(s)	3360
Ksp22I	TGATCA	3 cut(s)	1369, 2835, 3964
LmnI	GCTCC	5 cut(s)	496, 1056, 2561, 3883, 4105
MaeI	CTAG	6 cut(s)	744, 1812, 1962, 2625, 3648, 3887
MaeII	ACGT	4 cut(s)	87, 1052, 1995, 3367
MfeI	CAATTG	4 cut(s)	564, 1602, 1869, 3729
MflI	RGATCY	6 cut(s)	815, 1387, 2572, 3314, 3860, 3979
MhlI	GDGCHC	2 cut(s)	1061, 4102
MlyI	GAGTC	7 cut(s)	405, 1342, 2867, 3694, 3707, 3755, 4072
MmeI	TCCRAC	2 cut(s)	1536, 2466
Mph1103I	ATGCAT	1 cut(s)	1456
MroXI	GAANNNNTTC	3 cut(s)	1281, 2143, 3739
MslI	CAYNNNNRTG	3 cut(s)	317, 556, 2993
MspA1I	CMGCKG	1 cut(s)	71
MspCI	CTTAAG	2 cut(s)	1181, 4022
MspI	CCGG	4 cut(s)	186, 408, 3110, 3656
MunI	CAATTG	4 cut(s)	564, 1602, 1869, 3729
MvaI	CCWGG	8 cut(s)	339, 813, 1009, 1062, 1890, 2647, 2970, 3804
MwoI	GCNNNNNNNGC	9 cut(s)	77, 305, 722, 803, 1303, 2110, 2591, 3571, 4001
NciI	CCSGG	2 cut(s)	186, 187
NcoI	CCATGG	2 cut(s)	1198, 3588
NdeI	CATATG	1 cut(s)	1216
NlaIV	GGNNCC	4 cut(s)	1058, 2574, 2763, 3358
NmuCI	GTSAC	9 cut(s)	91, 344, 1530, 1986, 2132, 2207, 2321, 2354, 2859
NsbI	TGCGCA	2 cut(s)	3236, 3942
NsiI	ATGCAT	1 cut(s)	1456
NspI	RCATGY	8 cut(s)	531, 606, 678, 1225, 1777, 3012, 3350, 3955
NspV	TTCGAA	2 cut(s)	383, 2277
PaeI	GCATGC	2 cut(s)	531, 606
PaeR7I	CTCGAG	2 cut(s)	1399, 2553
PcsI	WCGNNNNNNNCGW	5 cut(s)	36, 39, 45, 48, 54
PdmI	GAANNNNTTC	3 cut(s)	1281, 2143, 3739
PfeI	GAWTC	6 cut(s)	1681, 2230, 2680, 3092, 3265, 3740
PflFI	GACNNNGTC	2 cut(s)	89, 2195
PflMI	CCANNNNNTGG	1 cut(s)	315
PfoI	TCCNGGA	2 cut(s)	1888, 3802
PinAI	ACCGGT	1 cut(s)	3109
PleI	GAGTC	7 cut(s)	405, 1341, 2866, 3694, 3707, 3754, 4071
PpsI	GAGTC	7 cut(s)	405, 1341, 2866, 3694, 3707, 3754, 4071
Ppu21I	YACGTR	1 cut(s)	3368
PpuMI	RGGWCCY	1 cut(s)	2407
Psp5II	RGGWCCY	1 cut(s)	2407
Psp6I	CCWGG	8 cut(s)	337, 811, 1007, 1060, 1888, 2645, 2968, 3802
PspEI	GGTNACC	1 cut(s)	1169
PspGI	CCWGG	8 cut(s)	337, 811, 1007, 1060, 1888, 2645, 2968, 3802
PspN4I	GGNNCC	4 cut(s)	1058, 2574, 2763, 3358
PspPI	GGNCC	3 cut(s)	1328, 1525, 2407
PspPPI	RGGWCCY	1 cut(s)	2407
PspXI	VCTCGAGB	2 cut(s)	1399, 2553
PstI	CTGCAG	2 cut(s)	436, 3916
PstNI	CAGNNNCTG	2 cut(s)	3159, 3911
PsuI	RGATCY	6 cut(s)	815, 1387, 2572, 3314, 3860, 3979
PsyI	GACNNNGTC	2 cut(s)	89, 2195
PvuII	CAGCTG	1 cut(s)	71
RsaI	GTAC	8 cut(s)	323, 2268, 3076, 3328, 3358, 3520, 3560, 3932
RsaNI	GTAC	8 cut(s)	322, 2267, 3075, 3327, 3357, 3519, 3559, 3931
RseI	CAYNNNNRTG	3 cut(s)	317, 556, 2993
SalI	GTCGAC	2 cut(s)	29, 83
Sau96I	GGNCC	3 cut(s)	1328, 1525, 2407
ScaI	AGTACT	4 cut(s)	2268, 3076, 3328, 3520
SchI	GAGTC	7 cut(s)	405, 1342, 2867, 3694, 3707, 3755, 4072
SduI	GDGCHC	2 cut(s)	1061, 4102
SfcI	CTRYAG	4 cut(s)	432, 1935, 3160, 3912
Sfr274I	CTCGAG	2 cut(s)	1399, 2553
SfuI	TTCGAA	2 cut(s)	383, 2277
SgrDI	CGTCGACG	1 cut(s)	83
SinI	GGWCC	2 cut(s)	1328, 2407
SlaI	CTCGAG	2 cut(s)	1399, 2553
SmaI	CCCGGG	1 cut(s)	187
SmiI	ATTTAAAT	1 cut(s)	1126
SmiMI	CAYNNNNRTG	3 cut(s)	317, 556, 2993
SmlI	CTYRAG	5 cut(s)	1181, 1399, 2047, 2553, 4022
SmoI	CTYRAG	5 cut(s)	1181, 1399, 2047, 2553, 4022
SpeI	ACTAGT	1 cut(s)	1961
SphI	GCATGC	2 cut(s)	531, 606
SsiI	CCGC	7 cut(s)	105, 231, 264, 770, 1019, 1738, 2615
SspI	AATATT	4 cut(s)	538, 689, 1518, 2883
SspMI	CTAG	6 cut(s)	744, 1812, 1962, 2625, 3648, 3887
StyI	CCWWGG	5 cut(s)	1198, 2307, 2618, 2819, 3588
SwaI	ATTTAAAT	1 cut(s)	1126
TaaI	ACNGT	8 cut(s)	832, 878, 1951, 2197, 3133, 3155, 3935, 4097
TaiI	ACGT	4 cut(s)	90, 1055, 1998, 3370
TatI	WGTACW	6 cut(s)	2266, 3074, 3326, 3518, 3558, 3930
TauI	GCSGC	3 cut(s)	267, 773, 1022
TfiI	GAWTC	6 cut(s)	1681, 2230, 2680, 3092, 3265, 3740
TseFI	GTSAC	9 cut(s)	91, 344, 1530, 1986, 2132, 2207, 2321, 2354, 2859
Tsp45I	GTSAC	9 cut(s)	91, 344, 1530, 1986, 2132, 2207, 2321, 2354, 2859
TspGWI	ACGGA	1 cut(s)	2957
TspMI	CCCGGG	1 cut(s)	185
Tth111I	GACNNNGTC	2 cut(s)	89, 2195
Van91I	CCANNNNNTGG	1 cut(s)	315
Vha464I	CTTAAG	2 cut(s)	1181, 4022
VpaK11BI	GGWCC	2 cut(s)	1328, 2407
XagI	CCTNNNNNAGG	1 cut(s)	2153
XbaI	TCTAGA	1 cut(s)	2624
XceI	RCATGY	8 cut(s)	531, 606, 678, 1225, 1777, 3012, 3350, 3955
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1127
XhoI	CTCGAG	2 cut(s)	1399, 2553
XmaI	CCCGGG	1 cut(s)	185
XmiI	GTMKAC	3 cut(s)	30, 84, 2446
XmnI	GAANNNNTTC	3 cut(s)	1281, 2143, 3739
XspI	CTAG	6 cut(s)	744, 1812, 1962, 2625, 3648, 3887
ZraI	GACGTC	1 cut(s)	88
ZrmI	AGTACT	4 cut(s)	2268, 3076, 3328, 3520
Zsp2I	ATGCAT	1 cut(s)	1456

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.