Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0002489

Physical Location & Seq

Genomic Coordinates Reverse (-)

54919442 .. 54929815

Gene Structure 10374 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG399900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGACCTCCACCGATCCTCACCACATAGTCGACGACGACGAAGACGACAATTTCGATTGGGAAGCAGCTGCCAGAGCCGTCGACGTCGTCACCCAGACCACCGCAAAGCCCACATCTTCCCATTTTACCCCTCCTCCCCCAATTCAGAAACCACCGTTTCCTCCAACCTTCAACAACAAGCCCAGTACTTCACGGCAGACCACTCTCGATAGGTTCATTGGGAAGCCCGCCCCAAATCCTCAGCCCGAAATTCGAGACTGCGGCGATGAGAGAGTGTCCCGTGTTCAAATTGATGCCGAAAAAGCCAACACTTGGATGTACCCAGTTAATTTTCCTGGGCGTGACTATCAATTTGCTATCACCAAGACAGCACTGTTTTCGAATACGCTGGTGGCATTGCCAACCGGACTCGGGAAGACGCTTATTGCTGCAGTTGTTATGTATAACTACTTCAGATGGTTCCCTGATGGAAAGATAGTCTTTGCTGCTCCTTCTCGACCACTTGTTACACAACAAATAGAAGCATGCCATAATATTGTAGGAATACCACAAGAATGGACAATTGATATGACAGGTCAGATAAGTCCTACAAAAAGAGCATGCTTTTGGAAAACTAAACGAGTTTTCTTTGTTACTCCACAAGTGCTGGAGAAGGACATTCAGTCTGGAACATGCTCAGTCGAATATTTGGTATGCTTGGTGATTGATGAAGCTCACCGAGCATTAGGGAACTATGCTTCTAGTGTTGCAGTTCGTGAGTTAATGGCGGCACAAGTGCCACTGAGAATTTTAGCTTTGACCGCAACACCAGGATCTAAGCAGCAGACTGTTCAGCAAATTATTGATAATTTGTGTATATCATCACTTGAATACCGTGAAGAAAATGATGCTGATGTCAGCCCTTATGTTCACAACAGGGAGGTAGAGTTGATTAAGGTGGCAATGGGTCAAGATGCTGTGGACATAAGTAATAAGATATATGATGCAATACGACCATATTTAACCAGGCTTTGTGCGGCTGGAGTTCTCCCAACTAGAGACGCTCAAACGTGGAGCCCAGGCGAGTTACTTAGGTTTAGGGAGAAATTTCGTCTTGCACCTCCTCCCGAGTTCCCCCAATTTAAATATGGAGAAATTGAAGGTTGGTATGGAGTTCTTCTTACAGGTTACCACATTCTTAAGCTATTATCAAGCCATGGAATAAGACCAGCATATGACATGCTTGAAGAAAAGTTAAAAAGGGGGTTTTTTGCCAAGTATATGAGTAGAAGTGAAGATGTTCATAAAGCAAAGCTAAGGATGCAGCAAAGTCTGTCTCATGGTGGTCCGAGTCCCAAGTTATCCAAAATGTTAGAAGTATTGCTTGATCATTTCAAAACAAAAGATCCACAAAACTCAAGGGTTATGATTTTCTCAAACTTCAGGGGAAGTGTCAGGGAGATAATGGATGCATTGGCAAACATTGGGAATTTAGTTAAAGCTACCGAGTTTATTGGTCAGAGTTCAGGGAAAATATTAAAAGGCCAGTCACAAAAAGTTCAACAAGCTGTGTTGGAGAAATTTCGTGCTGGTGGATACAATGTCATTGTTGCCACATCAATTGGCGAAGAGGGTTTGGATATAATGGAAGTCGATCTTGTTATATGCTTTGATGCAAATGTATCACCATTGAGGATGATTCAACGCATGGGGAGAACTGGAAGGAAGCATGATGGACGAGTTGATATCCTTTTCTTGAGAAACGCATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0031297 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043240 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045005 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071704 GO:0071821 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

Helicase_C ResIII

Protein Analysis

583

Amino Acids

65.41

Weight (kDa)

8.44

Isoelectric Point (pI)

47.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	120 - 275	8.1e-18	DEAD/DEAH box helicase
ResIII	PF04851	126 - 270	2.7e-18	Type III restriction enzyme, res subunit
Helicase_C	PF00271	453 - 569	3e-20	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G36020
fragaria_vesca	FvH4_7g03750
malus_domestica	MD15G1404400.v1.1
prunus_persica	Prupe.1G551100_v2.0.a1 Prupe.2G019600_v2.0.a1 Prupe.2G019700_v2.0.a1
pyrus_communis	pycom15g36170 pycom15g36180
rosa_chinensis	RchiOBHm_Chr7g0228871 RchiOBHm_Chr7g0228881 RchiOBHm_Chr7g0228951
rosa_laevigata	RLG00000001539 RLG00000002150 RLG00000022432
rosa_multiflora	Rmu_sc0000721.1_g000006 Rmu_sc0004517.1_g000001 Rmu_sc0005913.1_g000001 Rmu_sc0005913.1_g000026 Rmu_sc0011218.1_g000006 Rmu_sc0013945.1_g000010
rosa_roxburghii	Rroxscaffold_3G00230830 Rroxscaffold_3G00238230
rosa_rugosa	Rorug07G0252600 Rorug07G0254200
rosa_samantha	Rh7AG405800 Rh7BG333000 Rh7BG385100 Rh7BG385200 Rh7BG385300 Rh7CG423400 Rh7DG341000 Rh7DG399800 Rh7DG399900
rosa_wichuraiana	Rw7G033620 Rw7G033650 Rw7G033670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	87
AccB7I	CCANNNNNTGG	1 cut(s)	312
AccI	GTMKAC	2 cut(s)	30, 81
AciI	CCGC	6 cut(s)	102, 228, 261, 767, 801, 1016
AclWI	GGATC	3 cut(s)	8, 820, 1379
AcsI	RAATTY	5 cut(s)	249, 786, 1085, 1468, 1559
AcuI	CTGAAG	2 cut(s)	436, 1405
AcyI	GRCGYC	1 cut(s)	84
AfaI	GTAC	2 cut(s)	187, 320
AfiI	CCNNNNNNNGG	2 cut(s)	312, 411
AflII	CTTAAG	1 cut(s)	1178
AgsI	TTSAA	8 cut(s)	172, 287, 869, 1139, 1226, 1375, 1541, 1682
AhdI	GACNNNNNGTC	1 cut(s)	661
AjnI	CCWGG	4 cut(s)	334, 808, 1004, 1057
AluBI	AGCT	7 cut(s)	68, 713, 794, 1183, 1294, 1481, 1547
AluI	AGCT	7 cut(s)	68, 713, 794, 1183, 1294, 1481, 1547
Alw26I	GTCTC	3 cut(s)	249, 1032, 1320
AlwI	GGATC	3 cut(s)	8, 820, 1379
Ama87I	CYCGRG	2 cut(s)	410, 1106
AoxI	GGCC	1 cut(s)	1522
ApeKI	GCWGC	6 cut(s)	65, 68, 428, 485, 820, 1303
ApoI	RAATTY	5 cut(s)	249, 786, 1085, 1468, 1559
ArsI	GACNNNNNNTTYG	4 cut(s)	35, 67, 335, 367
Asp700I	GAANNNNTTC	1 cut(s)	1278
AspS9I	GGNCC	1 cut(s)	1325
AsuHPI	GGTGA	6 cut(s)	11, 82, 352, 707, 712, 1656
AsuII	TTCGAA	1 cut(s)	380
AvaI	CYCGRG	2 cut(s)	410, 1106
AvaII	GGWCC	1 cut(s)	1325
BanII	GRGCYC	1 cut(s)	1058
BarI	GAAGNNNNNNTAC	2 cut(s)	434, 466
BbsI	GAAGAC	2 cut(s)	48, 422
BbvCI	CCTCAGC	1 cut(s)	240
BbvI	GCAGC	6 cut(s)	55, 77, 415, 472, 832, 1315
BccI	CCATC	3 cut(s)	450, 461, 1706
BceAI	ACGGC	2 cut(s)	62, 209
BcgI	CGANNNNNNTGC	2 cut(s)	360, 394
BciT130I	CCWGG	4 cut(s)	336, 810, 1006, 1059
BciVI	GTATCC	1 cut(s)	1568
BclI	TGATCA	1 cut(s)	1366
BcoDI	GTCTC	3 cut(s)	249, 1032, 1320
BfaI	CTAG	2 cut(s)	741, 1035
BfmI	CTRYAG	1 cut(s)	429
BfrI	CTTAAG	1 cut(s)	1178
BfuI	GTATCC	1 cut(s)	1568
BisI	GCNGC	9 cut(s)	66, 69, 262, 429, 486, 768, 821, 1017, 1304
BlsI	GCNGC	9 cut(s)	67, 70, 263, 430, 487, 769, 822, 1018, 1305
BmcAI	AGTACT	1 cut(s)	187
Bme1390I	CCNGG	4 cut(s)	336, 810, 1006, 1059
Bme18I	GGWCC	1 cut(s)	1325
BmeRI	GACNNNNNGTC	1 cut(s)	661
BmeT110I	CYCGRG	2 cut(s)	410, 1106
BmgT120I	GGNCC	1 cut(s)	1325
BmiI	GGNNCC	2 cut(s)	461, 1055
BmrFI	CCNGG	4 cut(s)	336, 810, 1006, 1059
BmrI	ACTGGG	2 cut(s)	177, 317
BmsI	GCATC	7 cut(s)	283, 877, 943, 973, 1290, 1438, 1642
BmuI	ACTGGG	2 cut(s)	177, 317
BpiI	GAAGAC	2 cut(s)	48, 422
BpmI	CTGGAG	2 cut(s)	668, 1041
Bpu10I	CCTNAGC	2 cut(s)	240, 1295
Bpu14I	TTCGAA	1 cut(s)	380
BpuEI	CTTGAG	1 cut(s)	1381
BsaHI	GRCGYC	1 cut(s)	84
BsaJI	CCNNGG	3 cut(s)	335, 1057, 1195
BsaWI	WCCGGW	1 cut(s)	404
BsaXI	ACNNNNNCTCC	4 cut(s)	118, 148, 911, 941
Bsc4I	CCNNNNNNNGG	2 cut(s)	312, 411
Bse1I	ACTGG	4 cut(s)	183, 323, 1525, 1702
Bse3DI	GCAATG	2 cut(s)	395, 948
BseBI	CCWGG	4 cut(s)	336, 810, 1006, 1059
BseDI	CCNNGG	3 cut(s)	335, 1057, 1195
BseGI	GGATG	4 cut(s)	321, 1305, 1453, 1680
BseLI	CCNNNNNNNGG	2 cut(s)	312, 411
BseMI	GCAATG	2 cut(s)	395, 948
BseMII	CTCAG	3 cut(s)	254, 690, 773
BseNI	ACTGG	4 cut(s)	183, 323, 1525, 1702
BseRI	GAGGAG	2 cut(s)	123, 1092
BseXI	GCAGC	6 cut(s)	55, 77, 415, 472, 832, 1315
BshFI	GGCC	1 cut(s)	1524
BsiHKCI	CYCGRG	2 cut(s)	410, 1106
BsiSI	CCGG	1 cut(s)	405
BslFI	GGGAC	2 cut(s)	262, 1317
BslI	CCNNNNNNNGG	2 cut(s)	312, 411
BsmAI	GTCTC	3 cut(s)	249, 1032, 1320
BsmBI	CGTCTC	1 cut(s)	1032
BsmFI	GGGAC	2 cut(s)	262, 1317
BsnI	GGCC	1 cut(s)	1524
BsoBI	CYCGRG	2 cut(s)	410, 1106
Bsp119I	TTCGAA	1 cut(s)	380
Bsp1286I	GDGCHC	1 cut(s)	1058
Bsp143I	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
Bsp19I	CCATGG	1 cut(s)	1195
BspACI	CCGC	6 cut(s)	102, 228, 261, 767, 801, 1016
BspANI	GGCC	1 cut(s)	1524
BspCNI	CTCAG	3 cut(s)	253, 689, 774
BspLI	GGNNCC	2 cut(s)	461, 1055
BspMAI	CTGCAG	1 cut(s)	433
BspPI	GGATC	3 cut(s)	8, 820, 1379
BspT104I	TTCGAA	1 cut(s)	380
BspTI	CTTAAG	1 cut(s)	1178
BsrDI	GCAATG	2 cut(s)	395, 948
BsrI	ACTGG	4 cut(s)	183, 323, 1525, 1702
BssECI	CCNNGG	3 cut(s)	335, 1057, 1195
BssMI	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
BssNI	GRCGYC	1 cut(s)	84
BssT1I	CCWWGG	1 cut(s)	1195
Bst2UI	CCWGG	4 cut(s)	336, 810, 1006, 1059
Bst4CI	ACNGT	4 cut(s)	156, 375, 829, 875
Bst6I	CTCTTC	1 cut(s)	1602
BstACI	GRCGYC	1 cut(s)	84
BstAFI	CTTAAG	1 cut(s)	1178
BstBI	TTCGAA	1 cut(s)	380
BstC8I	GCNNGC	3 cut(s)	228, 526, 601
BstDEI	CTNAG	6 cut(s)	240, 676, 782, 816, 1070, 1295
BstDSI	CCRYGG	1 cut(s)	1195
BstEII	GGTNACC	1 cut(s)	1166
BstF5I	GGATG	4 cut(s)	321, 1305, 1453, 1680
BstKTI	GATC	5 cut(s)	16, 815, 1369, 1387, 1636
BstMAI	GTCTC	3 cut(s)	249, 1032, 1320
BstMBI	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 302, 719, 800, 1300
BstNI	CCWGG	4 cut(s)	336, 810, 1006, 1059
BstNSI	RCATGY	4 cut(s)	528, 603, 675, 1222
BstPI	GGTNACC	1 cut(s)	1166
BstSCI	CCNGG	4 cut(s)	334, 808, 1004, 1057
BstSFI	CTRYAG	1 cut(s)	429
BstV1I	GCAGC	6 cut(s)	55, 77, 415, 472, 832, 1315
BstV2I	GAAGAC	2 cut(s)	48, 422
BstX2I	RGATCY	2 cut(s)	812, 1384
BstXI	CCANNNNNNTGG	1 cut(s)	555
BstYI	RGATCY	2 cut(s)	812, 1384
BsuI	GTATCC	1 cut(s)	1568
BsuRI	GGCC	1 cut(s)	1524
BtgI	CCRYGG	1 cut(s)	1195
BtgZI	GCGATG	1 cut(s)	279
BtsCI	GGATG	4 cut(s)	321, 1305, 1453, 1680
BtsIMutI	CAGTG	2 cut(s)	371, 779
Cac8I	GCNNGC	3 cut(s)	228, 526, 601
Cfr13I	GGNCC	1 cut(s)	1325
CseI	GACGC	2 cut(s)	427, 1049
Csp6I	GTAC	2 cut(s)	186, 319
CspCI	CAANNNNNGTGG	2 cut(s)	1582, 1617
CviAII	CATG	8 cut(s)	525, 600, 672, 1196, 1219, 1319, 1687, 1709
CviQI	GTAC	2 cut(s)	186, 319
DdeI	CTNAG	6 cut(s)	240, 676, 782, 816, 1070, 1295
DpnI	GATC	5 cut(s)	15, 814, 1368, 1386, 1635
DpnII	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
DraI	TTTAAA	1 cut(s)	1123
DriI	GACNNNNNGTC	1 cut(s)	661
Eam1104I	CTCTTC	1 cut(s)	1602
Eam1105I	GACNNNNNGTC	1 cut(s)	661
EarI	CTCTTC	1 cut(s)	1602
Eco130I	CCWWGG	1 cut(s)	1195
Eco24I	GRGCYC	1 cut(s)	1058
Eco32I	GATATC	1 cut(s)	1726
Eco47I	GGWCC	1 cut(s)	1325
Eco57I	CTGAAG	2 cut(s)	436, 1405
Eco88I	CYCGRG	2 cut(s)	410, 1106
Eco91I	GGTNACC	1 cut(s)	1166
EcoO65I	GGTNACC	1 cut(s)	1166
EcoRII	CCWGG	4 cut(s)	334, 808, 1004, 1057
EcoRV	GATATC	1 cut(s)	1726
EcoT14I	CCWWGG	1 cut(s)	1195
EcoT22I	ATGCAT	1 cut(s)	1453
EcoT38I	GRGCYC	1 cut(s)	1058
ErhI	CCWWGG	1 cut(s)	1195
Esp3I	CGTCTC	1 cut(s)	1032
FaeI	CATG	8 cut(s)	528, 603, 675, 1199, 1222, 1322, 1690, 1712
FalI	AAGNNNNNCTT	6 cut(s)	464, 496, 1347, 1379, 1620, 1652
FaqI	GGGAC	2 cut(s)	262, 1317
FatI	CATG	8 cut(s)	524, 599, 671, 1195, 1218, 1318, 1686, 1708
FauI	CCCGC	1 cut(s)	235
FauNDI	CATATG	1 cut(s)	1213
FbaI	TGATCA	1 cut(s)	1366
FblI	GTMKAC	2 cut(s)	30, 81
Fnu4HI	GCNGC	9 cut(s)	66, 69, 262, 429, 486, 768, 821, 1017, 1304
FokI	GGATG	4 cut(s)	328, 1312, 1460, 1687
FriOI	GRGCYC	1 cut(s)	1058
Fsp4HI	GCNGC	9 cut(s)	66, 69, 262, 429, 486, 768, 821, 1017, 1304
FspBI	CTAG	2 cut(s)	741, 1035
GluI	GCNGC	9 cut(s)	66, 69, 262, 429, 486, 768, 821, 1017, 1304
GsuI	CTGGAG	2 cut(s)	668, 1041
HaeIII	GGCC	1 cut(s)	1524
HapII	CCGG	1 cut(s)	405
HgaI	GACGC	2 cut(s)	427, 1049
Hin1I	GRCGYC	1 cut(s)	84
Hin1II	CATG	8 cut(s)	528, 603, 675, 1199, 1222, 1322, 1690, 1712
HincII	GTYRAC	2 cut(s)	31, 82
HindII	GTYRAC	2 cut(s)	31, 82
HinfI	GANTC	3 cut(s)	408, 1330, 1678
HpaII	CCGG	1 cut(s)	405
HphI	GGTGA	6 cut(s)	11, 82, 352, 707, 712, 1656
Hpy166II	GTNNAC	4 cut(s)	31, 82, 910, 961
Hpy188I	TCNGA	5 cut(s)	147, 455, 579, 1329, 1500
Hpy188III	TCNNGA	9 cut(s)	206, 254, 412, 495, 666, 755, 950, 1106, 1735
Hpy8I	GTNNAC	4 cut(s)	31, 82, 910, 961
Hpy99I	CGWCG	6 cut(s)	35, 38, 41, 83, 86, 89
HpyAV	CCTTC	5 cut(s)	178, 501, 646, 1133, 1695
HpyCH4III	ACNGT	4 cut(s)	156, 375, 829, 875
HpyCH4IV	ACGT	2 cut(s)	84, 1049
HpyCH4V	TGCA	7 cut(s)	431, 749, 986, 1097, 1303, 1451, 1655
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 302, 719, 800, 1300
HpyF3I	CTNAG	6 cut(s)	240, 676, 782, 816, 1070, 1295
HpySE526I	ACGT	2 cut(s)	84, 1049
Hsp92I	GRCGYC	1 cut(s)	84
Hsp92II	CATG	8 cut(s)	528, 603, 675, 1199, 1222, 1322, 1690, 1712
Ksp22I	TGATCA	1 cut(s)	1366
Kzo9I	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
LmnI	GCTCC	2 cut(s)	493, 1053
Lsp1109I	GCAGC	6 cut(s)	55, 77, 415, 472, 832, 1315
LweI	GCATC	7 cut(s)	283, 877, 943, 973, 1290, 1438, 1642
MaeI	CTAG	2 cut(s)	741, 1035
MaeII	ACGT	2 cut(s)	84, 1049
MaeIII	GTNAC	7 cut(s)	88, 341, 505, 631, 1065, 1166, 1527
MalI	GATC	5 cut(s)	15, 814, 1368, 1386, 1635
MboI	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
MboII	GAAGA	8 cut(s)	53, 108, 427, 890, 1148, 1238, 1286, 1619
MfeI	CAATTG	2 cut(s)	561, 1599
MflI	RGATCY	2 cut(s)	812, 1384
MhlI	GDGCHC	1 cut(s)	1058
MlyI	GAGTC	2 cut(s)	402, 1339
MmeI	TCCRAC	2 cut(s)	188, 1533
Mph1103I	ATGCAT	1 cut(s)	1453
MroXI	GAANNNNTTC	1 cut(s)	1278
MslI	CAYNNNNRTG	2 cut(s)	314, 553
MspA1I	CMGCKG	1 cut(s)	68
MspCI	CTTAAG	1 cut(s)	1178
MspI	CCGG	1 cut(s)	405
MspR9I	CCNGG	4 cut(s)	336, 810, 1006, 1059
MunI	CAATTG	2 cut(s)	561, 1599
MvaI	CCWGG	4 cut(s)	336, 810, 1006, 1059
MwoI	GCNNNNNNNGC	5 cut(s)	74, 302, 719, 800, 1300
NcoI	CCATGG	1 cut(s)	1195
NdeI	CATATG	1 cut(s)	1213
NdeII	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
NlaIII	CATG	8 cut(s)	528, 603, 675, 1199, 1222, 1322, 1690, 1712
NlaIV	GGNNCC	2 cut(s)	461, 1055
NmuCI	GTSAC	3 cut(s)	88, 341, 1527
NsiI	ATGCAT	1 cut(s)	1453
NspI	RCATGY	4 cut(s)	528, 603, 675, 1222
NspV	TTCGAA	1 cut(s)	380
PaeI	GCATGC	2 cut(s)	528, 603
PcsI	WCGNNNNNNNCGW	3 cut(s)	36, 42, 51
PdmI	GAANNNNTTC	1 cut(s)	1278
PfeI	GAWTC	1 cut(s)	1678
PflFI	GACNNNGTC	1 cut(s)	86
PflMI	CCANNNNNTGG	1 cut(s)	312
PkrI	GCNGC	9 cut(s)	67, 70, 263, 430, 487, 769, 822, 1018, 1305
PleI	GAGTC	2 cut(s)	402, 1338
PpsI	GAGTC	2 cut(s)	402, 1338
Psp6I	CCWGG	4 cut(s)	334, 808, 1004, 1057
PspEI	GGTNACC	1 cut(s)	1166
PspGI	CCWGG	4 cut(s)	334, 808, 1004, 1057
PspN4I	GGNNCC	2 cut(s)	461, 1055
PspPI	GGNCC	1 cut(s)	1325
PstI	CTGCAG	1 cut(s)	433
PsuI	RGATCY	2 cut(s)	812, 1384
PsyI	GACNNNGTC	1 cut(s)	86
PvuII	CAGCTG	1 cut(s)	68
RsaI	GTAC	2 cut(s)	187, 320
RsaNI	GTAC	2 cut(s)	186, 319
RseI	CAYNNNNRTG	2 cut(s)	314, 553
SalI	GTCGAC	2 cut(s)	29, 80
SatI	GCNGC	9 cut(s)	66, 69, 262, 429, 486, 768, 821, 1017, 1304
Sau3AI	GATC	5 cut(s)	13, 812, 1366, 1384, 1633
Sau96I	GGNCC	1 cut(s)	1325
ScaI	AGTACT	1 cut(s)	187
SchI	GAGTC	2 cut(s)	402, 1339
ScrFI	CCNGG	4 cut(s)	336, 810, 1006, 1059
SduI	GDGCHC	1 cut(s)	1058
SfaNI	GCATC	7 cut(s)	283, 877, 943, 973, 1290, 1438, 1642
SfcI	CTRYAG	1 cut(s)	429
SfuI	TTCGAA	1 cut(s)	380
SgrDI	CGTCGACG	1 cut(s)	80
SinI	GGWCC	1 cut(s)	1325
SmiI	ATTTAAAT	1 cut(s)	1123
SmiMI	CAYNNNNRTG	2 cut(s)	314, 553
SmlI	CTYRAG	3 cut(s)	1178, 1396, 1735
SmoI	CTYRAG	3 cut(s)	1178, 1396, 1735
SphI	GCATGC	2 cut(s)	528, 603
SsiI	CCGC	6 cut(s)	102, 228, 261, 767, 801, 1016
SspI	AATATT	3 cut(s)	535, 686, 1515
SspMI	CTAG	2 cut(s)	741, 1035
StyD4I	CCNGG	4 cut(s)	334, 808, 1004, 1057
StyI	CCWWGG	1 cut(s)	1195
SwaI	ATTTAAAT	1 cut(s)	1123
TaaI	ACNGT	4 cut(s)	156, 375, 829, 875
TaiI	ACGT	2 cut(s)	87, 1052
TaqI	TCGA	9 cut(s)	30, 54, 81, 207, 253, 380, 496, 681, 1632
TatI	WGTACW	1 cut(s)	185
TauI	GCSGC	3 cut(s)	264, 770, 1019
TfiI	GAWTC	1 cut(s)	1678
TscAI	CASTG	2 cut(s)	378, 786
TseFI	GTSAC	3 cut(s)	88, 341, 1527
TseI	GCWGC	6 cut(s)	65, 68, 428, 485, 820, 1303
Tsp45I	GTSAC	3 cut(s)	88, 341, 1527
TspDTI	ATGAA	3 cut(s)	205, 723, 1271
TspRI	CASTG	2 cut(s)	378, 786
Tth111I	GACNNNGTC	1 cut(s)	86
Van91I	CCANNNNNTGG	1 cut(s)	312
Vha464I	CTTAAG	1 cut(s)	1178
VpaK11BI	GGWCC	1 cut(s)	1325
XapI	RAATTY	5 cut(s)	249, 786, 1085, 1468, 1559
XceI	RCATGY	4 cut(s)	528, 603, 675, 1222
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1124
XmiI	GTMKAC	2 cut(s)	30, 81
XmnI	GAANNNNTTC	1 cut(s)	1278
XspI	CTAG	2 cut(s)	741, 1035
ZraI	GACGTC	1 cut(s)	85
ZrmI	AGTACT	1 cut(s)	187
Zsp2I	ATGCAT	1 cut(s)	1453

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.