Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002489

Physical Location & Seq

Genomic Coordinates Forward (+)

26330401 .. 26342204

Gene Structure 11804 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002150

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4122 bp

ATGACCTCCACCGATCCCCACCACATAGTCGACGACGACGAAGACGACGATTTCGATTGGGAAGCAGCTGCCAGAGCCGTCGACGTCGTCACCCAGACCACCGCAAAGCCCTCAACTTCCCATTTTGCCCCTCCTTCCCCAATTCAGAAACCACCATTTCCTCCAATCTCCCACAACAAGCCCAGTACTTCACGGCAGACCACTCTCGATAGGTTCATTGGGAAGCCCGCCCCAAAGCCTCAGCCCGAAATTCGAGACTGCGGCGATGAGAGAGTGTCCCGTGTTCAAATTGATGCCGAAAAAGCCAATACTTGGATGTACCCAGTTAATTTTCCTGAGCGTGACTATCAATTTGCTATCACCAAGACAGCACTTTTTTCGAATACGCTGGTGGCATTGCCAACCGGACTCGGGAAGACGCTTATTGCTGCGGTTGTTATGTATAACTACTTCAGATGGTTCCCTGATGGAAAGATAGTCTTTGCTGCTCCTTCTCGACCACTTGTTACACAACAAATAGAAGCATGCCATAATATTGTAGGAATACCACAAGAATGGACAATTGATATGACAGGTCAGATAAGTCCTACAAAAAGAGCATGCTTTTGGAAAACTAAACGAGTTTTCTTTGTTACTCCACAAGTGCTGGAGAAGGACATTCAGTCTGGAACATGCTCAGTCGAATATTTGGTATGCTTGGTGATTGATGAAGCTCACCGAGCATTAGGGAACTATGCTTCTAGTGTTGCAGTTCGTGAGTTAATGGCAGCACAAGTGCCACTGAGAATTTTAGCTTTGACTGCAACACCAGGATCTAAGCAGCAGACTGTTCAGCAAATTATTGATAACTTGTGTATATCATCACTTGAATACCGTGAAGAAAATGATGCTGATGTCAGCCCTTATGTTCACAACAGGGAGGTAGAGTTGATTAAGGTGGCAATGGGTCAAGATGCTATGGAGATAAGTAATAAGATATATGATGCAATACAACCATATTTAACCAGGCTTAGTGCGGCTGGAGTTCTCCCAAATAGAGACGCTCAAACGTGGAGCCCAGGCGAGTTACTTACGTTTAGGGAGAAATTTCGTCTAGCACCTCCTCCAGACTTCCCCCAATTTAAGTATGGGGAAATTGAAGGTTGGTATGGAGTTCTTCTTACAGGTTACCACATTCTTAAGCTATTATCCAGCCATGGAATAAGACCAGCATATGACATGCTTGAAGAAAAGTTAAAAAAGGGGTTTTTTGCCAAGTATATGAGTAGAAGTGAAGATGTTCATAAAGCAAAGCTAAGGATGCAGCAAAGTCTGTCTCATGGTGGTCTGAGTCCCAAGTTATCCAAAATGTTAGAAGTATTGCTTGATCATTTCAAAACAAAAGATCCACAAAACTCGAGGGTTATGATTTTCTCAAACTTCAGGGGAAGTGTCAGGGAGATAATGGATGCATTGGCAAACATTGGGGATTTAGTTAGAGCTACCGAGTTTATTGGTCAGAGTTCAGGGAAAATATTAAAAGGCCAGTCACAAAAAGTTCAGCAAGCTGTGTTGGAGAAATTTCGTGCTGGTGGATACAATGTCATTGTTGCCACATCAATTGGCGAAGAAGGTTTGGATATAATGGAAGTTGATCTTGTTATATGCTTTGATGCAAATGTATCACCATTGAGGATGATTCAACGCATGGGGAGAACTGGAAGGAAGCATGATGGACGATTGAAAGGTTACAAGCGGAAGCTAACAAACAGCAAGAATGTGAGCAAACACATGCGAAATGGGGGAATAAATAGCTTCAATTTTCATTCTAGCCCGAGGATGATCCCTCACATTTTCAAACCTGAAGTCCAATATGTTGAGTTTTCAATTGAACAATTCGTTCGTCCTGGAAAGAAAGTCAAAGATGATAATGATATTCAGACACCTGTTACTATAGACAAATTAACTGTTGCTGAGACTAGTTTAGTTGCCAAGTATTTCCAGTCACATGACGTAACATGGAAACCATCTCTTATTGCCTTTCCTCACTTCCAAACCTATCCCTCAAGAGTGTATAAAGTAATGCACTCGCCTCGAACAACAATGCTAATTGATACAATGCAATCTTTGCAAGGATTATCCTTTTCCAGTGACAGAAAAATTTCCCTCATCGAGGATGAGGTCTGTTCAGAGGATTGCTTAGCAGTTGACACTGTCGAACAGCGTGACAACAGAGAAGATTTGCTGAATCTTGATGGTTCAAAGAAACAATCACAGGAAATAGTACTGGATTTCGAACTACAAACTACTGGGACATTGGAAACCAAGGAGAAGCATAGCGACCATGATTTATCTGATGAAAATCCTTCTGGTCACTCTTTTCTCTTTGGTTCTGATGTTGTTGATGTTGATGCTTCTGGAAAGGTCCTTATCATATCTGTCCCTATTCTTCATGGCAAAAGTATACCATATTCCAAGTTTACAAGTGCAAGCAGTGGTAAGTTGGATCGTTTGAAGCAAAGTTCTTGCCATGTAAGAACATCAGATGAAGAACAAACAGAAGTAACTACTCGAGCTGGAGCTAATGGGAATCCAGAAATGGCTCAGATAGAATATATAAGGAATGATATACCACCTTGGTCTAGATTGTGCGACTCTGAAACCTGGATGGAGACAACAATCAATGGGGATGCAGAGAATCCTCAAAAGCCAATTCTCAAAGGAAACTTTCCAAATGGAGATAGTGCTTGTGAGACGCCTGATGTTCTGAAAAGCAAGGCACCGACATTGCTGGCTGATGAAGACGACAACCTTTTCAGAGATGGTGAGCTTAGTCCAAGGCTTACTAATTTGATCAAAAGTGGAGTTATTCCAGAGTCACCTATCAATAATAGTGGAATATTAGATACCATAAATAAGTATGTAGTTCCTGATGCTGTTTCGCCTGCCCCATTACCTACGGAACTGATATCGAAGTCTTCAAGTCCTGGGAAAAATGGAAAAGCCACTGATATGGATACCAGTGCATGTGAAAGAAATGTGTCTCTTTTTCCTACTGATATTGAAATTCAATCTCCCTTGCACAATAGGAGTACTGCCAGTATAAATGAATCTACCTCCACGTCACCGGTTATTGACAGAGGCCAAACTGTTTTAGCAGACCTTACAAACAGTAGCTGTAGCAAAGATTGGTGTATAGGTTCTGGAGACAAGTCGCAGAGTGTGAAACAGACACGCAAGCTTAAAAGGTTGCGCAAAGTTGGGGATCATTGGAAAAGTAGGAATCAGGAAAGCTTGACAACCAATGATGTCCCAAGAGCAAACCTGACCAGATCTTTTATAAGTACTACTCTTCCTACTAAGCATCTTAGAGGTACCAAAATACGTGCAAAGAAGTCGGTTAATGATGTGAGGGAATTCATTGACGAGGAAGCTGAGGTATCTTCAGAGGATATATCTGATGATGAAGAAGATGAACCAGACAACTATTCAAACGATAGTTTCATAGATGACAGGATAAATTCTACGGCTGCAAGTACTCAGGCTGCAACCAGTGGAATTGACATGATGGCAGTGTACAGGCGTTCATTGCTCACCCAATCACCCATGGAGAGGCAGCCAAGCAGTTCAGCCACATTTTCTCCCAATTCTGTGGCAATGATAACTAGGACACCGGAAACTGGAAGTTCTGGTGGAAAAACATCTCTCTCTCTTCAGACTCCTCAAGGCGACTCTACAAATCTGCCAATTGGAATAGATTCAAAGTCTTTCCATAAGAATTGGAACTCTGAAGCCAGACCTTTTACAACTGGTGTTTCTCCAGAATATGAAAGAGATATTGAAAGTCGAAAAAGAAAATCGAGTTTTCATCATTCAAGATCTATTCCTGCTGTCAACTTGGAGCTAGAATTCTCGTTGCAATCAGAGGCTGCAGGCAGAAATATGCAGCACAGTGATGCACATGGGGACATGCTTTATGATGATCAGTTTTATGAGGGTCTTGATCTTGACGCATTGGAAGCACAAGCTACAATTCTTCTTAAACAGAAATCAGAATTGCCAGTGCAGAAGCCACAGATGAGTCGGTTAAACCCAGAAAATCCTACCCTTGACAGTGCTCCATCATTTGATCTTGGGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0000712 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007131 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0031297 GO:0031974 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0035825 GO:0036297 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043240 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045005 GO:0045132 GO:0046483 GO:0048285 GO:0050896 GO:0051304 GO:0051307 GO:0051321 GO:0051716 GO:0061982 GO:0070013 GO:0071704 GO:0071821 GO:0071840 GO:0090304 GO:0098813 GO:0140013 GO:1901360 GO:1901576 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko03460 map03460

Pfam Domains

Protein Families

Helicase_C ResIII

Protein Analysis

1374

Amino Acids

152.92

Weight (kDa)

5.78

Isoelectric Point (pI)

48.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	120 - 274	3.1e-17	DEAD/DEAH box helicase
ResIII	PF04851	126 - 270	1e-17	Type III restriction enzyme, res subunit
Helicase_C	PF00271	453 - 569	1.3e-19	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G35530 AT1G36020
fragaria_vesca	FvH4_7g03750
malus_domestica	MD15G1404400.v1.1
prunus_persica	Prupe.1G551100_v2.0.a1 Prupe.2G019600_v2.0.a1 Prupe.2G019700_v2.0.a1
pyrus_communis	pycom15g36170 pycom15g36180
rosa_chinensis	RchiOBHm_Chr7g0228871 RchiOBHm_Chr7g0228881 RchiOBHm_Chr7g0228951
rosa_laevigata	RLG00000001539 RLG00000002150 RLG00000022432
rosa_multiflora	Rmu_sc0000721.1_g000006 Rmu_sc0004517.1_g000001 Rmu_sc0005913.1_g000001 Rmu_sc0005913.1_g000026 Rmu_sc0011218.1_g000006 Rmu_sc0013945.1_g000010
rosa_roxburghii	Rroxscaffold_3G00230830 Rroxscaffold_3G00238230
rosa_rugosa	Rorug07G0252600 Rorug07G0254200
rosa_samantha	Rh7AG405800 Rh7BG333000 Rh7BG385100 Rh7BG385200 Rh7BG385300 Rh7CG423400 Rh7DG341000 Rh7DG399800 Rh7DG399900
rosa_wichuraiana	Rw7G033620 Rw7G033650 Rw7G033670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3320
AatII	GACGTC	1 cut(s)	87
Acc16I	TGCGCA	1 cut(s)	3233
Acc65I	GGTACC	1 cut(s)	3353
AccB1I	GGYRCC	2 cut(s)	2758, 3353
AccB7I	CCANNNNNTGG	1 cut(s)	312
AccI	GTMKAC	3 cut(s)	30, 81, 2443
AciI	CCGC	6 cut(s)	102, 228, 261, 431, 1016, 1735
AclWI	GGATC	6 cut(s)	8, 820, 1379, 1816, 2493, 3252
AcsI	RAATTY	9 cut(s)	249, 786, 1085, 1559, 2139, 3045, 3395, 3499, 3887
AcuI	CTGAAG	6 cut(s)	436, 1405, 1863, 3408, 3677, 3789
AcyI	GRCGYC	2 cut(s)	84, 2735
AfaI	GTAC	8 cut(s)	187, 320, 2265, 3073, 3325, 3355, 3517, 3557
AfiI	CCNNNNNNNGG	5 cut(s)	312, 411, 2152, 3591, 3659
AflII	CTTAAG	1 cut(s)	1178
AgeI	ACCGGT	1 cut(s)	3106
AhdI	GACNNNNNGTC	1 cut(s)	661
AhlI	ACTAGT	1 cut(s)	1958
AjiI	CACGTC	1 cut(s)	3102
AjnI	CCWGG	6 cut(s)	808, 1004, 1057, 1885, 2642, 2965
Alw21I	GWGCWC	1 cut(s)	4099
Alw26I	GTCTC	8 cut(s)	249, 1032, 1320, 1949, 2645, 2726, 3027, 3180
AlwI	GGATC	6 cut(s)	8, 820, 1379, 1816, 2493, 3252
AlwNI	CAGNNNCTG	2 cut(s)	3156, 3908
Ama87I	CYCGRG	4 cut(s)	410, 1396, 1813, 2550
AoxI	GGCC	2 cut(s)	1522, 3121
ApoI	RAATTY	9 cut(s)	249, 786, 1085, 1559, 2139, 3045, 3395, 3499, 3887
ArsI	GACNNNNNNTTYG	6 cut(s)	35, 67, 335, 367, 1830, 1862
AsiGI	ACCGGT	1 cut(s)	3106
Asp700I	GAANNNNTTC	3 cut(s)	1278, 2140, 3736
Asp718I	GGTACC	1 cut(s)	3353
AspLEI	GCGC	1 cut(s)	3234
AspS9I	GGNCC	1 cut(s)	2404
AsuII	TTCGAA	2 cut(s)	380, 2274
AvaI	CYCGRG	4 cut(s)	410, 1396, 1813, 2550
AvaII	GGWCC	1 cut(s)	2404
BanI	GGYRCC	2 cut(s)	2758, 3353
BanII	GRGCYC	1 cut(s)	1058
BarI	GAAGNNNNNNTAC	2 cut(s)	434, 466
BbsI	GAAGAC	4 cut(s)	48, 422, 2787, 2949
Bbv12I	GWGCWC	1 cut(s)	4099
BbvCI	CCTCAGC	2 cut(s)	240, 3414
BccI	CCATC	9 cut(s)	450, 461, 1706, 2014, 2228, 2641, 2795, 3541, 4108
BceAI	ACGGC	3 cut(s)	62, 209, 3522
BcgI	CGANNNNNNTGC	6 cut(s)	360, 394, 2054, 2088, 3357, 3391
BciT130I	CCWGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
BciVI	GTATCC	2 cut(s)	1568, 2989
BclI	TGATCA	3 cut(s)	1366, 2832, 3961
BcoDI	GTCTC	8 cut(s)	249, 1032, 1320, 1949, 2645, 2726, 3027, 3180
BcuI	ACTAGT	1 cut(s)	1958
BfaI	CTAG	7 cut(s)	741, 1094, 1809, 1959, 2622, 3645, 3884
BfmI	CTRYAG	3 cut(s)	1932, 3157, 3909
BfrI	CTTAAG	1 cut(s)	1178
BfuI	GTATCC	2 cut(s)	1568, 2989
BglII	AGATCT	2 cut(s)	3311, 3857
BlpI	GCTNAGC	1 cut(s)	2179
BmcAI	AGTACT	5 cut(s)	187, 2265, 3073, 3325, 3517
Bme1390I	CCNGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
Bme18I	GGWCC	1 cut(s)	2404
BmeRI	GACNNNNNGTC	1 cut(s)	661
BmeT110I	CYCGRG	4 cut(s)	410, 1396, 1813, 2550
BmgBI	CACGTC	1 cut(s)	3102
BmgT120I	GGNCC	1 cut(s)	2404
BmiI	GGNNCC	4 cut(s)	461, 1055, 2760, 3355
BmrFI	CCNGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
BmrI	ACTGGG	3 cut(s)	177, 317, 2298
BmuI	ACTGGG	3 cut(s)	177, 317, 2298
BpiI	GAAGAC	4 cut(s)	48, 422, 2787, 2949
BpmI	CTGGAG	6 cut(s)	668, 1041, 1089, 2577, 3204, 3783
Bpu10I	CCTNAGC	4 cut(s)	240, 336, 1295, 3414
Bpu1102I	GCTNAGC	1 cut(s)	2179
Bpu14I	TTCGAA	2 cut(s)	380, 2274
BpuEI	CTTGAG	2 cut(s)	2029, 3687
BsaAI	YACGTR	1 cut(s)	3365
BsaBI	GATNNNNATC	1 cut(s)	2340
BsaHI	GRCGYC	2 cut(s)	84, 2735
BsaJI	CCNNGG	8 cut(s)	1057, 1195, 1814, 2304, 2615, 2816, 2966, 3585
BsaWI	WCCGGW	3 cut(s)	404, 3106, 3652
BsaXI	ACNNNNNCTCC	4 cut(s)	911, 941, 3604, 3634
Bsc4I	CCNNNNNNNGG	5 cut(s)	312, 411, 2152, 3591, 3659
Bse118I	RCCGGY	1 cut(s)	3106
Bse3DI	GCAATG	5 cut(s)	395, 948, 2765, 3566, 3642
Bse8I	GATNNNNATC	1 cut(s)	2340
BseBI	CCWGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
BseDI	CCNNGG	8 cut(s)	1057, 1195, 1814, 2304, 2615, 2816, 2966, 3585
BseGI	GGATG	8 cut(s)	321, 1305, 1453, 1680, 1824, 2161, 2652, 2674
BseJI	GATNNNNATC	1 cut(s)	2340
BseLI	CCNNNNNNNGG	5 cut(s)	312, 411, 2152, 3591, 3659
BseMI	GCAATG	5 cut(s)	395, 948, 2765, 3566, 3642
BseMII	CTCAG	9 cut(s)	254, 327, 690, 773, 1319, 1944, 2597, 3405, 3533
BseRI	GAGGAG	2 cut(s)	1092, 3690
BsgI	GTGCAG	1 cut(s)	4064
BshFI	GGCC	2 cut(s)	1524, 3123
BshNI	GGYRCC	2 cut(s)	2758, 3353
BshTI	ACCGGT	1 cut(s)	3106
BsiHKAI	GWGCWC	1 cut(s)	4099
BsiHKCI	CYCGRG	4 cut(s)	410, 1396, 1813, 2550
BsiSI	CCGG	3 cut(s)	405, 3107, 3653
BslFI	GGGAC	6 cut(s)	262, 1317, 2305, 2405, 3275, 3959
BslI	CCNNNNNNNGG	5 cut(s)	312, 411, 2152, 3591, 3659
BsmAI	GTCTC	8 cut(s)	249, 1032, 1320, 1949, 2645, 2726, 3027, 3180
BsmBI	CGTCTC	2 cut(s)	1032, 2726
BsmFI	GGGAC	6 cut(s)	262, 1317, 2305, 2405, 3275, 3959
BsnI	GGCC	2 cut(s)	1524, 3123
BsoBI	CYCGRG	4 cut(s)	410, 1396, 1813, 2550
Bsp119I	TTCGAA	2 cut(s)	380, 2274
Bsp1286I	GDGCHC	2 cut(s)	1058, 4099
Bsp1407I	TGTACA	1 cut(s)	3555
Bsp1720I	GCTNAGC	1 cut(s)	2179
Bsp19I	CCATGG	2 cut(s)	1195, 3585
BspACI	CCGC	6 cut(s)	102, 228, 261, 431, 1016, 1735
BspANI	GGCC	2 cut(s)	1524, 3123
BspCNI	CTCAG	9 cut(s)	253, 328, 689, 774, 1320, 1945, 2596, 3406, 3532
BspLI	GGNNCC	4 cut(s)	461, 1055, 2760, 3355
BspMAI	CTGCAG	1 cut(s)	3913
BspPI	GGATC	6 cut(s)	8, 820, 1379, 1816, 2493, 3252
BspT104I	TTCGAA	2 cut(s)	380, 2274
BspT107I	GGYRCC	2 cut(s)	2758, 3353
BspTI	CTTAAG	1 cut(s)	1178
BsrDI	GCAATG	5 cut(s)	395, 948, 2765, 3566, 3642
BsrFI	RCCGGY	1 cut(s)	3106
BsrGI	TGTACA	1 cut(s)	3555
BssAI	RCCGGY	1 cut(s)	3106
BssECI	CCNNGG	8 cut(s)	1057, 1195, 1814, 2304, 2615, 2816, 2966, 3585
BssNAI	GTATAC	1 cut(s)	2444
BssNI	GRCGYC	2 cut(s)	84, 2735
BssT1I	CCWWGG	5 cut(s)	1195, 2304, 2615, 2816, 3585
Bst1107I	GTATAC	1 cut(s)	2444
Bst2UI	CCWGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
Bst4CI	ACNGT	8 cut(s)	829, 875, 1948, 2194, 3130, 3152, 3932, 4094
Bst6I	CTCTTC	2 cut(s)	3336, 3696
BstACI	GRCGYC	2 cut(s)	84, 2735
BstAFI	CTTAAG	1 cut(s)	1178
BstAPI	GCANNNNNTGC	1 cut(s)	2107
BstAUI	TGTACA	1 cut(s)	3555
BstBAI	YACGTR	1 cut(s)	3365
BstBI	TTCGAA	2 cut(s)	380, 2274
BstC8I	GCNNGC	9 cut(s)	228, 526, 601, 1545, 2470, 2772, 2925, 3218, 3913
BstDSI	CCRYGG	2 cut(s)	1195, 3585
BstEII	GGTNACC	1 cut(s)	1166
BstENI	CCTNNNNNAGG	1 cut(s)	2150
BstF5I	GGATG	8 cut(s)	321, 1305, 1453, 1680, 1824, 2161, 2652, 2674
BstHHI	GCGC	1 cut(s)	3234
BstMAI	GTCTC	8 cut(s)	249, 1032, 1320, 1949, 2645, 2726, 3027, 3180
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 302, 719, 800, 1300, 2107, 3568, 3998
BstNI	CCWGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
BstNSI	RCATGY	7 cut(s)	528, 603, 675, 1222, 1774, 3009, 3952
BstPI	GGTNACC	1 cut(s)	1166
BstSCI	CCNGG	6 cut(s)	808, 1004, 1057, 1885, 2642, 2965
BstSFI	CTRYAG	3 cut(s)	1932, 3157, 3909
BstV2I	GAAGAC	4 cut(s)	48, 422, 2787, 2949
BstX2I	RGATCY	4 cut(s)	812, 1384, 3311, 3857
BstXI	CCANNNNNNTGG	3 cut(s)	555, 2992, 3631
BstYI	RGATCY	4 cut(s)	812, 1384, 3311, 3857
BstZ17I	GTATAC	1 cut(s)	2444
BsuI	GTATCC	2 cut(s)	1568, 2989
BsuRI	GGCC	2 cut(s)	1524, 3123
BtgI	CCRYGG	2 cut(s)	1195, 3585
BtgZI	GCGATG	1 cut(s)	279
BtrI	CACGTC	1 cut(s)	3102
BtsCI	GGATG	8 cut(s)	321, 1305, 1453, 1680, 1824, 2161, 2652, 2674
BtsI	GCAGTG	2 cut(s)	2479, 3558
Cac8I	GCNNGC	9 cut(s)	228, 526, 601, 1545, 2470, 2772, 2925, 3218, 3913
CaiI	CAGNNNCTG	2 cut(s)	3156, 3908
CfoI	GCGC	1 cut(s)	3234
Cfr10I	RCCGGY	1 cut(s)	3106
Cfr13I	GGNCC	1 cut(s)	2404
CseI	GACGC	4 cut(s)	427, 1049, 2743, 3998
Csp6I	GTAC	8 cut(s)	186, 319, 2264, 3072, 3324, 3354, 3516, 3556
CspAI	ACCGGT	1 cut(s)	3106
CspCI	CAANNNNNGTGG	4 cut(s)	1582, 1617, 2854, 2889
CviQI	GTAC	8 cut(s)	186, 319, 2264, 3072, 3324, 3354, 3516, 3556
DriI	GACNNNNNGTC	1 cut(s)	661
Eam1104I	CTCTTC	2 cut(s)	3336, 3696
Eam1105I	GACNNNNNGTC	1 cut(s)	661
EarI	CTCTTC	2 cut(s)	3336, 3696
Eco130I	CCWWGG	5 cut(s)	1195, 2304, 2615, 2816, 3585
Eco24I	GRGCYC	1 cut(s)	1058
Eco32I	GATATC	1 cut(s)	2949
Eco47I	GGWCC	1 cut(s)	2404
Eco57I	CTGAAG	6 cut(s)	436, 1405, 1863, 3408, 3677, 3789
Eco88I	CYCGRG	4 cut(s)	410, 1396, 1813, 2550
Eco91I	GGTNACC	1 cut(s)	1166
EcoNI	CCTNNNNNAGG	1 cut(s)	2150
EcoO109I	RGGNCCY	1 cut(s)	2404
EcoO65I	GGTNACC	1 cut(s)	1166
EcoRI	GAATTC	2 cut(s)	3395, 3887
EcoRII	CCWGG	6 cut(s)	808, 1004, 1057, 1885, 2642, 2965
EcoRV	GATATC	1 cut(s)	2949
EcoT14I	CCWWGG	5 cut(s)	1195, 2304, 2615, 2816, 3585
EcoT22I	ATGCAT	1 cut(s)	1453
EcoT38I	GRGCYC	1 cut(s)	1058
ErhI	CCWWGG	5 cut(s)	1195, 2304, 2615, 2816, 3585
Esp3I	CGTCTC	2 cut(s)	1032, 2726
FalI	AAGNNNNNCTT	6 cut(s)	464, 496, 1347, 1379, 1620, 1652
FaqI	GGGAC	6 cut(s)	262, 1317, 2305, 2405, 3275, 3959
FauI	CCCGC	1 cut(s)	235
FauNDI	CATATG	1 cut(s)	1213
FbaI	TGATCA	3 cut(s)	1366, 2832, 3961
FblI	GTMKAC	3 cut(s)	30, 81, 2443
FokI	GGATG	8 cut(s)	328, 1312, 1460, 1687, 1831, 2168, 2659, 2681
FriOI	GRGCYC	1 cut(s)	1058
FspBI	CTAG	7 cut(s)	741, 1094, 1809, 1959, 2622, 3645, 3884
FspI	TGCGCA	1 cut(s)	3233
GlaI	GCGC	1 cut(s)	3233
GsuI	CTGGAG	6 cut(s)	668, 1041, 1089, 2577, 3204, 3783
HaeIII	GGCC	2 cut(s)	1524, 3123
HapII	CCGG	3 cut(s)	405, 3107, 3653
HgaI	GACGC	4 cut(s)	427, 1049, 2743, 3998
HhaI	GCGC	1 cut(s)	3234
Hin1I	GRCGYC	2 cut(s)	84, 2735
Hin6I	GCGC	1 cut(s)	3232
HinP1I	GCGC	1 cut(s)	3232
HincII	GTYRAC	4 cut(s)	31, 82, 2188, 3874
HindII	GTYRAC	4 cut(s)	31, 82, 2188, 3874
HindIII	AAGCTT	2 cut(s)	3218, 3271
HpaII	CCGG	3 cut(s)	405, 3107, 3653
Hpy166II	GTNNAC	8 cut(s)	31, 82, 910, 2188, 2444, 2460, 3556, 3874
Hpy8I	GTNNAC	8 cut(s)	31, 82, 910, 2188, 2444, 2460, 3556, 3874
Hpy99I	CGWCG	7 cut(s)	35, 38, 41, 50, 83, 86, 89
HpyAV	CCTTC	7 cut(s)	144, 501, 646, 1133, 1604, 1695, 2355
HpyCH4III	ACNGT	8 cut(s)	829, 875, 1948, 2194, 3130, 3152, 3932, 4094
HpyCH4IV	ACGT	6 cut(s)	84, 1049, 1073, 1992, 3101, 3364
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 302, 719, 800, 1300, 2107, 3568, 3998
HpySE526I	ACGT	6 cut(s)	84, 1049, 1073, 1992, 3101, 3364
Hsp92I	GRCGYC	2 cut(s)	84, 2735
HspAI	GCGC	1 cut(s)	3232
KpnI	GGTACC	1 cut(s)	3357
Ksp22I	TGATCA	3 cut(s)	1366, 2832, 3961
LmnI	GCTCC	5 cut(s)	493, 1053, 2558, 3880, 4102
MaeI	CTAG	7 cut(s)	741, 1094, 1809, 1959, 2622, 3645, 3884
MaeII	ACGT	6 cut(s)	84, 1049, 1073, 1992, 3101, 3364
MfeI	CAATTG	4 cut(s)	561, 1599, 1866, 3726
MflI	RGATCY	4 cut(s)	812, 1384, 3311, 3857
MhlI	GDGCHC	2 cut(s)	1058, 4099
MlyI	GAGTC	7 cut(s)	402, 1339, 2627, 2864, 3691, 3704, 4069
MmeI	TCCRAC	2 cut(s)	1533, 2463
Mph1103I	ATGCAT	1 cut(s)	1453
MroXI	GAANNNNTTC	3 cut(s)	1278, 2140, 3736
MslI	CAYNNNNRTG	3 cut(s)	553, 2990, 3933
MspA1I	CMGCKG	1 cut(s)	68
MspCI	CTTAAG	1 cut(s)	1178
MspI	CCGG	3 cut(s)	405, 3107, 3653
MspR9I	CCNGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
MunI	CAATTG	4 cut(s)	561, 1599, 1866, 3726
MvaI	CCWGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
MwoI	GCNNNNNNNGC	8 cut(s)	74, 302, 719, 800, 1300, 2107, 3568, 3998
NcoI	CCATGG	2 cut(s)	1195, 3585
NdeI	CATATG	1 cut(s)	1213
NlaIV	GGNNCC	4 cut(s)	461, 1055, 2760, 3355
NmuCI	GTSAC	9 cut(s)	88, 341, 1527, 1983, 2129, 2204, 2351, 2856, 3102
NsbI	TGCGCA	1 cut(s)	3233
NsiI	ATGCAT	1 cut(s)	1453
NspI	RCATGY	7 cut(s)	528, 603, 675, 1222, 1774, 3009, 3952
NspV	TTCGAA	2 cut(s)	380, 2274
PaeI	GCATGC	2 cut(s)	528, 603
PaeR7I	CTCGAG	2 cut(s)	1396, 2550
PcsI	WCGNNNNNNNCGW	4 cut(s)	36, 42, 45, 51
PdmI	GAANNNNTTC	3 cut(s)	1278, 2140, 3736
PfeI	GAWTC	7 cut(s)	1678, 2227, 2569, 2677, 3089, 3262, 3737
PflFI	GACNNNGTC	2 cut(s)	86, 2192
PflMI	CCANNNNNTGG	1 cut(s)	312
PfoI	TCCNGGA	1 cut(s)	1885
PinAI	ACCGGT	1 cut(s)	3106
PleI	GAGTC	7 cut(s)	402, 1338, 2627, 2863, 3691, 3704, 4068
PpsI	GAGTC	7 cut(s)	402, 1338, 2627, 2863, 3691, 3704, 4068
Ppu21I	YACGTR	1 cut(s)	3365
PpuMI	RGGWCCY	1 cut(s)	2404
PsiI	TTATAA	1 cut(s)	3320
Psp5II	RGGWCCY	1 cut(s)	2404
Psp6I	CCWGG	6 cut(s)	808, 1004, 1057, 1885, 2642, 2965
PspEI	GGTNACC	1 cut(s)	1166
PspGI	CCWGG	6 cut(s)	808, 1004, 1057, 1885, 2642, 2965
PspN4I	GGNNCC	4 cut(s)	461, 1055, 2760, 3355
PspPI	GGNCC	1 cut(s)	2404
PspPPI	RGGWCCY	1 cut(s)	2404
PspXI	VCTCGAGB	2 cut(s)	1396, 2550
PstI	CTGCAG	1 cut(s)	3913
PstNI	CAGNNNCTG	2 cut(s)	3156, 3908
PsuI	RGATCY	4 cut(s)	812, 1384, 3311, 3857
PsyI	GACNNNGTC	2 cut(s)	86, 2192
PvuII	CAGCTG	1 cut(s)	68
RsaI	GTAC	8 cut(s)	187, 320, 2265, 3073, 3325, 3355, 3517, 3557
RsaNI	GTAC	8 cut(s)	186, 319, 2264, 3072, 3324, 3354, 3516, 3556
RseI	CAYNNNNRTG	3 cut(s)	553, 2990, 3933
SalI	GTCGAC	2 cut(s)	29, 80
Sau96I	GGNCC	1 cut(s)	2404
ScaI	AGTACT	5 cut(s)	187, 2265, 3073, 3325, 3517
SchI	GAGTC	7 cut(s)	402, 1339, 2627, 2864, 3691, 3704, 4069
ScrFI	CCNGG	6 cut(s)	810, 1006, 1059, 1887, 2644, 2967
SduI	GDGCHC	2 cut(s)	1058, 4099
SfcI	CTRYAG	3 cut(s)	1932, 3157, 3909
Sfr274I	CTCGAG	2 cut(s)	1396, 2550
SfuI	TTCGAA	2 cut(s)	380, 2274
SgrDI	CGTCGACG	1 cut(s)	80
SinI	GGWCC	1 cut(s)	2404
SlaI	CTCGAG	2 cut(s)	1396, 2550
SmiMI	CAYNNNNRTG	3 cut(s)	553, 2990, 3933
SmlI	CTYRAG	5 cut(s)	1178, 1396, 2044, 2550, 3702
SmoI	CTYRAG	5 cut(s)	1178, 1396, 2044, 2550, 3702
SpeI	ACTAGT	1 cut(s)	1958
SphI	GCATGC	2 cut(s)	528, 603
SsiI	CCGC	6 cut(s)	102, 228, 261, 431, 1016, 1735
SspI	AATATT	4 cut(s)	535, 686, 1515, 2880
SspMI	CTAG	7 cut(s)	741, 1094, 1809, 1959, 2622, 3645, 3884
StyD4I	CCNGG	6 cut(s)	808, 1004, 1057, 1885, 2642, 2965
StyI	CCWWGG	5 cut(s)	1195, 2304, 2615, 2816, 3585
TaaI	ACNGT	8 cut(s)	829, 875, 1948, 2194, 3130, 3152, 3932, 4094
TaiI	ACGT	6 cut(s)	87, 1052, 1076, 1995, 3104, 3367
TatI	WGTACW	6 cut(s)	185, 2263, 3071, 3323, 3515, 3555
TauI	GCSGC	2 cut(s)	264, 1019
TfiI	GAWTC	7 cut(s)	1678, 2227, 2569, 2677, 3089, 3262, 3737
TseFI	GTSAC	9 cut(s)	88, 341, 1527, 1983, 2129, 2204, 2351, 2856, 3102
Tsp45I	GTSAC	9 cut(s)	88, 341, 1527, 1983, 2129, 2204, 2351, 2856, 3102
TspGWI	ACGGA	1 cut(s)	2954
Tth111I	GACNNNGTC	2 cut(s)	86, 2192
Van91I	CCANNNNNTGG	1 cut(s)	312
Vha464I	CTTAAG	1 cut(s)	1178
VpaK11BI	GGWCC	1 cut(s)	2404
XagI	CCTNNNNNAGG	1 cut(s)	2150
XapI	RAATTY	9 cut(s)	249, 786, 1085, 1559, 2139, 3045, 3395, 3499, 3887
XbaI	TCTAGA	1 cut(s)	2621
XceI	RCATGY	7 cut(s)	528, 603, 675, 1222, 1774, 3009, 3952
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1124
XhoI	CTCGAG	2 cut(s)	1396, 2550
XmiI	GTMKAC	3 cut(s)	30, 81, 2443
XmnI	GAANNNNTTC	3 cut(s)	1278, 2140, 3736
XspI	CTAG	7 cut(s)	741, 1094, 1809, 1959, 2622, 3645, 3884
ZraI	GACGTC	1 cut(s)	85
ZrmI	AGTACT	5 cut(s)	187, 2265, 3073, 3325, 3517
Zsp2I	ATGCAT	1 cut(s)	1453

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.