AT2G17010

mechanosensitive ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001376
Physical Location & Seq
Reverse (-)
7392762 .. 7395985
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G17010.1

Sequence Viewer

Length: 2727 bp
ATGGATTTCAGAAATTCCTTCAAATCTCATAGCTCATACAAACAAATTCGAAGCCCCGGAGATCAAAGCGAGCCAAGCCCTGAACATCTGCCAATTCTCCACGATCACCATCCTGATCATTCCGGCATGGTGGTCGATGACCAGAAACCCGACAGCACTCGTTCTAGCCTCGACGATGGTCGTAACGCGCCTGTAGAACGTGATGCCAGTTACAAATTCTGGCAAGACAACACTACTGGAACGTCAACGGATCACACGGCGGTGAGAACAAGTGATAAAGATCCAATCGCTATTAGTCGAAAAGGCGATAGATTAAGCGGTAGTTTCGATTTCGTGCACGGGAAGCTACCGGTTGATGAATCTCCAACGAAAATGGTCGCTGGAGAGCCTGTGAATCGGCAATGGAGAGGTAGAAATAATGAAGAGATCACACTTGATGTTGATCAAGAAAATGATGATGTAAGTCACCAAACAATGCCTACGCCTACATCAACGGCTCGAACCTCGTTTGATGCGTCTAGAGAAATGCGAGTCTCTTTTAACGTTCGTAGAGCGGGTGGTGCGTTTGTCGCTGGTTCGGTTCCTTCTTCGTCTTCCCATTCGTCTTCTTCTTCGTCCGCGACAATGCGGACGAATCAGGATCAGCCGCAACTGCAGGAGGAAGAGGTTGTGAGATGTACTTCGAACATGTCGTTTCAGAGGAAATCAGAGCTTATATCTAGAGTGAAGACAAGGTCTAGGCTTCAAGATCCGCCACGTGAGGAAGAAACGCCTTACTCGGGTTGGAGATCGGGTCAGTTGAAATCCGGGTTACTTGCGGATATTGATGAAGAGGATGATCCATTAGCGGAAGAAGATGTACCAGATGAGTATAAAAGAGGGAAGCTTGACGCCATTACATTGCTCCAGTGGCTTAGCTTAGTCGCTATTATAGCTGCATTAGCGTGTAGTTTGTCGATTCAGTCTTGGAAGAAAGTTAGAGTTTGGAATCTTCATCTATGGAAATGGGAAGTGTTCTTGTTGGTTCTTATATGTGGGAGGTTGGTTTCGGGTTGGGGAATCCGAATCGTTGTCTTCTTCATCGAGAGAAACTTCCTTTTGCGGAAGCGGGTTCTTTACTTCGTTTACGGTGTGCGTAGAGCTGTTCAGAACTGTCTATGGCTAGGCCTAGTCCTTCTCGCTTGGCACTTCTTGTTCGACAAGAAAGTACAGAGGGAGACAAGGAGCAGGTTTCTTCCTTACGTAACCAAGATCTTGGTGTGTTTCTTGCTAAGTACTATCTTGTGGTTGATCAAGACACTAGTGGTTAAAGTTCTGGCCTCTTCGTTCCACGTCAGTACTTACTTTGATCGGATTCAAGAAGCGCTCTTTAACCAGTACGTGATCGAGACGTTATCGGGTCCTCCCATGATTGAAATGAGCAGGATTGAGGAAGAGGAAGAGCGGGCGCAAGACGAGATATTCAAGATGCAGAACGCAGGTGCTAATTTACCACCAGATCTTTGCGCGGCTGCGTTTCCACCGGGAAAAAGCGGGAGAGTAATGAATCCGAAACTCTCCCCGATAATCCCGAAATCAACAACTGATAATGGAATTAGCATGGAACATCTTCACAGGATGAATCATAAGAACATCTCTGCTTGGAACATGAAGAGACTAATGAAGATTGTGAGAAATGTTTCTCTGACCACACTGGACGAACAGATGCTAGAAAGCACATACGAAGATGAATCCACCCGCCAGATACGAAGCGAAAAAGAGGCTAAAGCAGCTGCAAGGAAGATTTTCAAGAACGTTGAGCAACGTGGCGCAAAGTACATTTACCTAGAGGATTTGATGAGATTTTTGCGGGAAGACGAGGCGATGAAGACCATGGGCCTCTTCGAAGGTGCACCGGAGAATAAAAGGATCAGCAAATCAGCCTTAAAGAACTGGCTGGTCAATGCTTTCAGAGAGCGACGAGCTCTTGCCCTGACACTCAACGACACCAAGACAGCAGTGAACAAGCTCCATCACATGATTAATATTGTCACTGCCATAGTCATTGTTGTCATTTGGCTTGTCCTTCTTGAAATTGCTTCCTCCAAGGTACTTCTCTTTGTAAGTTCACAAGTTGTACTCTTGGCCTTCATCTTTGGGAACACTGTCAAGACCGTCTTCGAGTCCATCATCTTCTTATTCATCGTACACCCTTACGATGTTGGTGATCGGTGTGAGATTGACAGTGTACAGTTGGTGGTGGAGGAGATGAACATACTCACTACCGTGTTCTTACGATACGACAATCTGAAGATTATGTATCCGAATAGTCTTTTGTGGCAGAAATCAATCAACAATTACTACCGCAGTCCGGATATGGGAGATGCAATCGAGTTCTGTGTCCACATTACTACTCCTCTTGAAAAAATCTCCGTGATCAAACAGAGAATATCGAACTACATCGACAACAAGCCGGAGTATTGGTACCCACAAGCCAAAATCATTGTAAAAGATTTGGAAGATTTGCACATAGTAAGACTAGCAATATGGCCATGTCACAGGATTAATCACCAAGACATGGCTGAAAGATGGACAAGAAGAGCCGTCTTAGTCGAAGAAGTGATTAAGATACTCCTCGAGCTCGACATTCAACACCGGTTTTATCCGCTCGATATCAATGTCCGAACAATGCCTACCGTTGTCTCTAGCAGAGTTCCACCTGGCTGGTCACAAAACCAACCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0016020 GO:0044464 GO:0051179 GO:0051234 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel

Protein Analysis

908

Amino Acids

104.31

Weight (kDa)

7.95

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 715 - 781 7.4e-10 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1469
Acc36I ACCTGC 2 cut(s) 1218, 1469
Acc65I GGTACC 1 cut(s) 2463
AccB1I GGYRCC 1 cut(s) 2463
AccB7I CCANNNNNTGG 1 cut(s) 2557
AccBSI CCGCTC 3 cut(s) 554, 1444, 2647
AccII CGCG 3 cut(s) 188, 620, 1508
AccIII TCCGGA 1 cut(s) 2350
AclI AACGTT 2 cut(s) 543, 1794
AclWI GGATC 6 cut(s) 258, 275, 648, 743, 833, 1916
AcoI YGGCCR 1 cut(s) 2528
AcsI RAATTY 3 cut(s) 13, 45, 215
AcuI CTGAAG 1 cut(s) 2309
AcvI CACGTG 1 cut(s) 758
AcyI GRCGYC 1 cut(s) 891
AfeI AGCGCT 1 cut(s) 1365
AfiI CCNNNNNNNGG 4 cut(s) 779, 2350, 2357, 2557
AflIII ACRYGT 1 cut(s) 687
AgeI ACCGGT 2 cut(s) 349, 2634
AhlI ACTAGT 1 cut(s) 1300
AjiI CACGTC 1 cut(s) 1333
AjnI CCWGG 1 cut(s) 2698
AleI CACNNNNGTG 2 cut(s) 260, 2264
Alw21I GWGCWC 4 cut(s) 339, 1894, 1966, 2622
Alw26I GTCTC 5 cut(s) 538, 1211, 1382, 1648, 2686
Alw44I GTGCAC 2 cut(s) 335, 1890
AlwI GGATC 6 cut(s) 258, 275, 648, 743, 833, 1916
Ama87I CYCGRG 2 cut(s) 778, 2615
Aor13HI TCCGGA 1 cut(s) 2350
Aor51HI AGCGCT 1 cut(s) 1365
AoxI GGCC 5 cut(s) 1165, 1317, 1876, 2124, 2528
ApaLI GTGCAC 2 cut(s) 335, 1890
ApeKI GCWGC 4 cut(s) 935, 1511, 1769, 1772
ApoI RAATTY 3 cut(s) 13, 45, 215
AseI ATTAAT 2 cut(s) 2022, 2544
AsiGI ACCGGT 2 cut(s) 349, 2634
Asp700I GAANNNNTTC 3 cut(s) 1608, 1678, 1784
Asp718I GGTACC 1 cut(s) 2463
AspLEI GCGC 5 cut(s) 190, 1366, 1450, 1508, 1811
AspS9I GGNCC 2 cut(s) 1400, 1876
AsuC2I CCSGG 3 cut(s) 57, 808, 1524
AsuHPI GGTGA 5 cut(s) 98, 274, 458, 2216, 2540
AsuII TTCGAA 3 cut(s) 49, 683, 1884
AvaI CYCGRG 2 cut(s) 778, 2615
AvaII GGWCC 1 cut(s) 1400
BaeGI GKGCMC 2 cut(s) 339, 1894
BalI TGGCCA 1 cut(s) 2530
BanI GGYRCC 1 cut(s) 2463
BanII GRGCYC 2 cut(s) 1966, 2622
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
BbrPI CACGTG 1 cut(s) 758
BbsI GAAGAC 7 cut(s) 585, 597, 734, 1066, 1860, 1874, 2149
Bbv12I GWGCWC 4 cut(s) 339, 1894, 1966, 2622
BbvI GCAGC 4 cut(s) 922, 1498, 1759, 1781
BccI CCATC 5 cut(s) 117, 170, 2019, 2174, 2562
BceAI ACGGC 3 cut(s) 273, 510, 2567
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 1 cut(s) 2700
BciVI GTATCC 1 cut(s) 2310
BclI TGATCA 4 cut(s) 115, 442, 1290, 2415
BcnI CCSGG 3 cut(s) 57, 808, 1524
BcoDI GTCTC 5 cut(s) 538, 1211, 1382, 1648, 2686
BcuI ACTAGT 1 cut(s) 1300
BfmI CTRYAG 2 cut(s) 192, 653
BfoI RGCGCY 1 cut(s) 1367
BfuAI ACCTGC 2 cut(s) 1218, 1469
BfuI GTATCC 1 cut(s) 2310
BglII AGATCT 2 cut(s) 1251, 1498
BisI GCNGC 6 cut(s) 647, 936, 1509, 1512, 1770, 1773
BlpI GCTNAGC 1 cut(s) 914
BlsI GCNGC 6 cut(s) 648, 937, 1510, 1513, 1771, 1774
BmcAI AGTACT 2 cut(s) 1276, 1339
Bme1390I CCNGG 4 cut(s) 57, 808, 1524, 2700
Bme18I GGWCC 1 cut(s) 1400
BmeT110I CYCGRG 2 cut(s) 778, 2615
BmgBI CACGTC 1 cut(s) 1333
BmgT120I GGNCC 2 cut(s) 1400, 1876
BmiI GGNNCC 3 cut(s) 582, 1401, 2465
BmrFI CCNGG 4 cut(s) 57, 808, 1524, 2700
BmsI GCATC 5 cut(s) 193, 502, 1458, 1695, 2353
BoxI GACNNNNGTC 1 cut(s) 177
BpiI GAAGAC 7 cut(s) 585, 597, 734, 1066, 1860, 1874, 2149
BpmI CTGGAG 2 cut(s) 402, 890
Bpu1102I GCTNAGC 1 cut(s) 914
Bpu14I TTCGAA 3 cut(s) 49, 683, 1884
BpuMI CCSGG 3 cut(s) 57, 808, 1524
BsaAI YACGTR 3 cut(s) 758, 1243, 1381
BsaBI GATNNNNATC 3 cut(s) 108, 279, 441
BsaHI GRCGYC 1 cut(s) 891
BsaJI CCNNGG 3 cut(s) 55, 1872, 2085
BsaWI WCCGGW 4 cut(s) 349, 1894, 2350, 2634
BsaXI ACNNNNNCTCC 6 cut(s) 375, 405, 778, 808, 2447, 2477
Bsc4I CCNNNNNNNGG 4 cut(s) 779, 2350, 2357, 2557
Bse118I RCCGGY 2 cut(s) 349, 2634
Bse1I ACTGG 6 cut(s) 207, 241, 907, 1375, 1698, 1937
Bse3DI GCAATG 2 cut(s) 407, 899
Bse8I GATNNNNATC 3 cut(s) 108, 279, 441
BseAI TCCGGA 1 cut(s) 2350
BseBI CCWGG 1 cut(s) 2700
BseDI CCNNGG 3 cut(s) 55, 1872, 2085
BseGI GGATG 3 cut(s) 109, 841, 1623
BseJI GATNNNNATC 3 cut(s) 108, 279, 441
BseLI CCNNNNNNNGG 4 cut(s) 779, 2350, 2357, 2557
BseMI GCAATG 2 cut(s) 407, 899
BseNI ACTGG 6 cut(s) 207, 241, 907, 1375, 1698, 1937
BseRI GAGGAG 3 cut(s) 2258, 2385, 2603
BseSI GKGCMC 2 cut(s) 339, 1894
BseXI GCAGC 4 cut(s) 922, 1498, 1759, 1781
Bsh1236I CGCG 3 cut(s) 188, 620, 1508
BshFI GGCC 5 cut(s) 1167, 1319, 1878, 2126, 2530
BshNI GGYRCC 1 cut(s) 2463
BshTI ACCGGT 2 cut(s) 349, 2634
BsiHKAI GWGCWC 4 cut(s) 339, 1894, 1966, 2622
BsiHKCI CYCGRG 2 cut(s) 778, 2615
BsiSI CCGG 9 cut(s) 57, 123, 350, 807, 1523, 1895, 2351, 2453, 2635
BslI CCNNNNNNNGG 4 cut(s) 779, 2350, 2357, 2557
BsmAI GTCTC 5 cut(s) 538, 1211, 1382, 1648, 2686
BsmBI CGTCTC 1 cut(s) 1382
BsnI GGCC 5 cut(s) 1167, 1319, 1878, 2126, 2530
BsoBI CYCGRG 2 cut(s) 778, 2615
Bsp119I TTCGAA 3 cut(s) 49, 683, 1884
Bsp1286I GDGCHC 4 cut(s) 339, 1894, 1966, 2622
Bsp13I TCCGGA 1 cut(s) 2350
Bsp1407I TGTACA 1 cut(s) 2227
Bsp1720I GCTNAGC 1 cut(s) 914
Bsp19I CCATGG 1 cut(s) 1872
BspANI GGCC 5 cut(s) 1167, 1319, 1878, 2126, 2530
BspEI TCCGGA 1 cut(s) 2350
BspFNI CGCG 3 cut(s) 188, 620, 1508
BspLI GGNNCC 3 cut(s) 582, 1401, 2465
BspMAI CTGCAG 1 cut(s) 657
BspMI ACCTGC 2 cut(s) 1218, 1469
BspPI GGATC 6 cut(s) 258, 275, 648, 743, 833, 1916
BspQI GCTCTTC 2 cut(s) 1434, 2572
BspT104I TTCGAA 3 cut(s) 49, 683, 1884
BspT107I GGYRCC 1 cut(s) 2463
BsrBI CCGCTC 3 cut(s) 554, 1444, 2647
BsrDI GCAATG 2 cut(s) 407, 899
BsrFI RCCGGY 2 cut(s) 349, 2634
BsrGI TGTACA 1 cut(s) 2227
BsrI ACTGG 6 cut(s) 207, 241, 907, 1375, 1698, 1937
BssAI RCCGGY 2 cut(s) 349, 2634
BssECI CCNNGG 3 cut(s) 55, 1872, 2085
BssNI GRCGYC 1 cut(s) 891
BssT1I CCWWGG 2 cut(s) 1872, 2085
Bst2UI CCWGG 1 cut(s) 2700
Bst4CI ACNGT 8 cut(s) 1130, 1154, 2146, 2155, 2225, 2232, 2266, 2677
Bst6I CTCTTC 9 cut(s) 417, 657, 825, 1327, 1428, 1434, 1646, 1886, 2572
BstACI GRCGYC 1 cut(s) 891
BstAUI TGTACA 1 cut(s) 2227
BstBAI YACGTR 3 cut(s) 758, 1243, 1381
BstBI TTCGAA 3 cut(s) 49, 683, 1884
BstC8I GCNNGC 2 cut(s) 71, 1446
BstDEI CTNAG 4 cut(s) 914, 919, 1271, 2587
BstDSI CCRYGG 1 cut(s) 1872
BstF5I GGATG 3 cut(s) 109, 841, 1623
BstFNI CGCG 3 cut(s) 188, 620, 1508
BstH2I RGCGCY 1 cut(s) 1367
BstHHI GCGC 5 cut(s) 190, 1366, 1450, 1508, 1811
BstMAI GTCTC 5 cut(s) 538, 1211, 1382, 1648, 2686
BstMWI GCNNNNNNNGC 9 cut(s) 75, 343, 560, 569, 652, 910, 932, 941, 1769
BstNI CCWGG 1 cut(s) 2700
BstNSI RCATGY 1 cut(s) 691
BstPAI GACNNNNGTC 1 cut(s) 177
BstSCI CCNGG 4 cut(s) 55, 806, 1522, 2698
BstSFI CTRYAG 2 cut(s) 192, 653
BstSLI GKGCMC 2 cut(s) 339, 1894
BstSNI TACGTA 1 cut(s) 1243
BstUI CGCG 3 cut(s) 188, 620, 1508
BstV1I GCAGC 4 cut(s) 922, 1498, 1759, 1781
BstV2I GAAGAC 7 cut(s) 585, 597, 734, 1066, 1860, 1874, 2149
BstX2I RGATCY 4 cut(s) 280, 748, 1251, 1498
BstXI CCANNNNNNTGG 2 cut(s) 1255, 2703
BstYI RGATCY 4 cut(s) 280, 748, 1251, 1498
BsuI GTATCC 1 cut(s) 2310
BsuRI GGCC 5 cut(s) 1167, 1319, 1878, 2126, 2530
BtgI CCRYGG 1 cut(s) 1872
BtgZI GCGATG 1 cut(s) 1877
BtrI CACGTC 1 cut(s) 1333
BtsCI GGATG 3 cut(s) 109, 841, 1623
BtsI GCAGTG 2 cut(s) 2004, 2031
BtsIMutI CAGTG 6 cut(s) 914, 1691, 2004, 2031, 2142, 2230
BveI ACCTGC 2 cut(s) 1218, 1469
Cac8I GCNNGC 2 cut(s) 71, 1446
CfoI GCGC 5 cut(s) 190, 1366, 1450, 1508, 1811
Cfr10I RCCGGY 2 cut(s) 349, 2634
Cfr13I GGNCC 2 cut(s) 1400, 1876
CseI GACGC 2 cut(s) 504, 899
CspAI ACCGGT 2 cut(s) 349, 2634
CviAII CATG 9 cut(s) 127, 688, 1408, 1600, 1648, 1873, 2017, 2532, 2557
DdeI CTNAG 4 cut(s) 914, 919, 1271, 2587
EaeI YGGCCR 1 cut(s) 2528
Eam1104I CTCTTC 9 cut(s) 417, 657, 825, 1327, 1428, 1434, 1646, 1886, 2572
EarI CTCTTC 9 cut(s) 417, 657, 825, 1327, 1428, 1434, 1646, 1886, 2572
EciI GGCGGA 1 cut(s) 741
Ecl136II GAGCTC 2 cut(s) 1964, 2620
Eco105I TACGTA 1 cut(s) 1243
Eco130I CCWWGG 2 cut(s) 1872, 2085
Eco147I AGGCCT 1 cut(s) 1167
Eco24I GRGCYC 2 cut(s) 1966, 2622
Eco32I GATATC 1 cut(s) 2653
Eco47I GGWCC 1 cut(s) 1400
Eco47III AGCGCT 1 cut(s) 1365
Eco53kI GAGCTC 2 cut(s) 1964, 2620
Eco57I CTGAAG 1 cut(s) 2309
Eco72I CACGTG 1 cut(s) 758
Eco88I CYCGRG 2 cut(s) 778, 2615
EcoICRI GAGCTC 2 cut(s) 1964, 2620
EcoO109I RGGNCCY 1 cut(s) 1400
EcoRII CCWGG 1 cut(s) 2698
EcoRV GATATC 1 cut(s) 2653
EcoT14I CCWWGG 2 cut(s) 1872, 2085
EcoT38I GRGCYC 2 cut(s) 1966, 2622
ErhI CCWWGG 2 cut(s) 1872, 2085
Esp3I CGTCTC 1 cut(s) 1382
FaeI CATG 9 cut(s) 130, 691, 1411, 1603, 1651, 1876, 2020, 2535, 2560
FalI AAGNNNNNCTT 2 cut(s) 2141, 2173
FatI CATG 9 cut(s) 126, 687, 1407, 1599, 1647, 1872, 2016, 2531, 2556
FauI CCCGC 6 cut(s) 547, 1101, 1437, 1526, 1745, 1842
FbaI TGATCA 4 cut(s) 115, 442, 1290, 2415
Fnu4HI GCNGC 6 cut(s) 647, 936, 1509, 1512, 1770, 1773
FokI GGATG 3 cut(s) 96, 848, 1630
FriOI GRGCYC 2 cut(s) 1966, 2622
Fsp4HI GCNGC 6 cut(s) 647, 936, 1509, 1512, 1770, 1773
GlaI GCGC 5 cut(s) 189, 1365, 1449, 1507, 1810
GluI GCNGC 6 cut(s) 647, 936, 1509, 1512, 1770, 1773
GsuI CTGGAG 2 cut(s) 402, 890
HaeII RGCGCY 1 cut(s) 1367
HaeIII GGCC 5 cut(s) 1167, 1319, 1878, 2126, 2530
HapII CCGG 9 cut(s) 57, 123, 350, 807, 1523, 1895, 2351, 2453, 2635
HgaI GACGC 2 cut(s) 504, 899
HhaI GCGC 5 cut(s) 190, 1366, 1450, 1508, 1811
Hin1I GRCGYC 1 cut(s) 891
Hin1II CATG 9 cut(s) 130, 691, 1411, 1603, 1651, 1876, 2020, 2535, 2560
Hin6I GCGC 5 cut(s) 188, 1364, 1448, 1506, 1809
HinP1I GCGC 5 cut(s) 188, 1364, 1448, 1506, 1809
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 884
HpaII CCGG 9 cut(s) 57, 123, 350, 807, 1523, 1895, 2351, 2453, 2635
HphI GGTGA 5 cut(s) 98, 274, 458, 2216, 2540
Hpy166II GTNNAC 9 cut(s) 246, 337, 1126, 1892, 2002, 2108, 2188, 2228, 2383
Hpy8I GTNNAC 9 cut(s) 246, 337, 1126, 1892, 2002, 2108, 2188, 2228, 2383
Hpy99I CGWCG 2 cut(s) 176, 1962
HpyAV CCTTC 6 cut(s) 28, 594, 1184, 1880, 2075, 2137
HpyCH4III ACNGT 8 cut(s) 1130, 1154, 2146, 2155, 2225, 2232, 2266, 2677
HpyCH4V TGCA 8 cut(s) 337, 655, 938, 1471, 1775, 1892, 2366, 2506
HpyF10VI GCNNNNNNNGC 9 cut(s) 75, 343, 560, 569, 652, 910, 932, 941, 1769
HpyF3I CTNAG 4 cut(s) 914, 919, 1271, 2587
Hsp92I GRCGYC 1 cut(s) 891
Hsp92II CATG 9 cut(s) 130, 691, 1411, 1603, 1651, 1876, 2020, 2535, 2560
HspAI GCGC 5 cut(s) 188, 1364, 1448, 1506, 1809
Kpn2I TCCGGA 1 cut(s) 2350
KpnI GGTACC 1 cut(s) 2467
Ksp22I TGATCA 4 cut(s) 115, 442, 1290, 2415
LguI GCTCTTC 2 cut(s) 1434, 2572
LmnI GCTCC 3 cut(s) 909, 1224, 2013
Lsp1109I GCAGC 4 cut(s) 922, 1498, 1759, 1781
LweI GCATC 5 cut(s) 193, 502, 1458, 1695, 2353
MaeIII GTNAC 8 cut(s) 182, 209, 464, 810, 1243, 2029, 2534, 2706
MbiI CCGCTC 3 cut(s) 554, 1444, 2647
MflI RGATCY 4 cut(s) 280, 748, 1251, 1498
MhlI GDGCHC 4 cut(s) 339, 1894, 1966, 2622
MlsI TGGCCA 1 cut(s) 2530
MluCI AATT 8 cut(s) 13, 45, 93, 215, 1486, 1593, 2073, 2335
MluNI TGGCCA 1 cut(s) 2530
MlyI GAGTC 2 cut(s) 540, 2171
MmeI TCCRAC 2 cut(s) 389, 764
Mox20I TGGCCA 1 cut(s) 2530
MroI TCCGGA 1 cut(s) 2350
MroXI GAANNNNTTC 3 cut(s) 1608, 1678, 1784
MscI TGGCCA 1 cut(s) 2530
MseI TTAA 8 cut(s) 314, 540, 1308, 1371, 1925, 2022, 2544, 2604
MslI CAYNNNNRTG 3 cut(s) 260, 943, 2264
Msp20I TGGCCA 1 cut(s) 2530
MspA1I CMGCKG 1 cut(s) 1772
MspI CCGG 9 cut(s) 57, 123, 350, 807, 1523, 1895, 2351, 2453, 2635
MspR9I CCNGG 4 cut(s) 57, 808, 1524, 2700
MvaI CCWGG 1 cut(s) 2700
MvnI CGCG 3 cut(s) 188, 620, 1508
MwoI GCNNNNNNNGC 9 cut(s) 75, 343, 560, 569, 652, 910, 932, 941, 1769
NciI CCSGG 3 cut(s) 57, 808, 1524
NcoI CCATGG 1 cut(s) 1872
NlaIII CATG 9 cut(s) 130, 691, 1411, 1603, 1651, 1876, 2020, 2535, 2560
NlaIV GGNNCC 3 cut(s) 582, 1401, 2465
NmuCI GTSAC 4 cut(s) 464, 2029, 2534, 2706
NspI RCATGY 1 cut(s) 691
NspV TTCGAA 3 cut(s) 49, 683, 1884
OliI CACNNNNGTG 2 cut(s) 260, 2264
PaeR7I CTCGAG 1 cut(s) 2615
PaqCI CACCTGC 1 cut(s) 1469
PceI AGGCCT 1 cut(s) 1167
PciI ACATGT 1 cut(s) 687
PciSI GCTCTTC 2 cut(s) 1434, 2572
PcsI WCGNNNNNNNCGW 2 cut(s) 512, 689
PdmI GAANNNNTTC 3 cut(s) 1608, 1678, 1784
PflFI GACNNNGTC 1 cut(s) 733
PflMI CCANNNNNTGG 1 cut(s) 2557
PinAI ACCGGT 2 cut(s) 349, 2634
PkrI GCNGC 6 cut(s) 648, 937, 1510, 1513, 1771, 1774
PleI GAGTC 2 cut(s) 539, 2170
PmaCI CACGTG 1 cut(s) 758
PmlI CACGTG 1 cut(s) 758
PpsI GAGTC 2 cut(s) 539, 2170
Ppu21I YACGTR 3 cut(s) 758, 1243, 1381
PpuMI RGGWCCY 1 cut(s) 1400
PscI ACATGT 1 cut(s) 687
PshAI GACNNNNGTC 1 cut(s) 177
PshBI ATTAAT 2 cut(s) 2022, 2544
Psp124BI GAGCTC 2 cut(s) 1966, 2622
Psp1406I AACGTT 2 cut(s) 543, 1794
Psp5II RGGWCCY 1 cut(s) 1400
Psp6I CCWGG 1 cut(s) 2698
PspCI CACGTG 1 cut(s) 758
PspGI CCWGG 1 cut(s) 2698
PspN4I GGNNCC 3 cut(s) 582, 1401, 2465
PspPI GGNCC 2 cut(s) 1400, 1876
PspPPI RGGWCCY 1 cut(s) 1400
PspXI VCTCGAGB 1 cut(s) 2615
PsrI GAACNNNNNNTAC 2 cut(s) 2656, 2688
PstI CTGCAG 1 cut(s) 657
PsuI RGATCY 4 cut(s) 280, 748, 1251, 1498
PsyI GACNNNGTC 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 1772
RseI CAYNNNNRTG 3 cut(s) 260, 943, 2264
SacI GAGCTC 2 cut(s) 1966, 2622
SapI GCTCTTC 2 cut(s) 1434, 2572
SaqAI TTAA 8 cut(s) 314, 540, 1308, 1371, 1925, 2022, 2544, 2604
SatI GCNGC 6 cut(s) 647, 936, 1509, 1512, 1770, 1773
Sau96I GGNCC 2 cut(s) 1400, 1876
ScaI AGTACT 2 cut(s) 1276, 1339
SchI GAGTC 2 cut(s) 540, 2171
ScrFI CCNGG 4 cut(s) 57, 808, 1524, 2700
SduI GDGCHC 4 cut(s) 339, 1894, 1966, 2622
SfaNI GCATC 5 cut(s) 193, 502, 1458, 1695, 2353
SfcI CTRYAG 2 cut(s) 192, 653
Sfr274I CTCGAG 1 cut(s) 2615
SfuI TTCGAA 3 cut(s) 49, 683, 1884
SinI GGWCC 1 cut(s) 1400
SlaI CTCGAG 1 cut(s) 2615
SmiMI CAYNNNNRTG 3 cut(s) 260, 943, 2264
SmlI CTYRAG 1 cut(s) 2615
SmoI CTYRAG 1 cut(s) 2615
SnaBI TACGTA 1 cut(s) 1243
SpeI ACTAGT 1 cut(s) 1300
Sse9I AATT 8 cut(s) 13, 45, 93, 215, 1486, 1593, 2073, 2335
SseBI AGGCCT 1 cut(s) 1167
SspI AATATT 1 cut(s) 2026
SstI GAGCTC 2 cut(s) 1966, 2622
StuI AGGCCT 1 cut(s) 1167
StyD4I CCNGG 4 cut(s) 55, 806, 1522, 2698
StyI CCWWGG 2 cut(s) 1872, 2085
TaaI ACNGT 8 cut(s) 1130, 1154, 2146, 2155, 2225, 2232, 2266, 2677
TasI AATT 8 cut(s) 13, 45, 93, 215, 1486, 1593, 2073, 2335
TatI WGTACW 7 cut(s) 677, 1207, 1274, 1337, 1815, 2116, 2227
TauI GCSGC 2 cut(s) 649, 1511
Tru1I TTAA 8 cut(s) 314, 540, 1308, 1371, 1925, 2022, 2544, 2604
Tru9I TTAA 8 cut(s) 314, 540, 1308, 1371, 1925, 2022, 2544, 2604
TscAI CASTG 6 cut(s) 914, 1698, 2004, 2038, 2149, 2230
TseFI GTSAC 4 cut(s) 464, 2029, 2534, 2706
TseI GCWGC 4 cut(s) 935, 1511, 1769, 1772
Tsp45I GTSAC 4 cut(s) 464, 2029, 2534, 2706
TspGWI ACGGA 2 cut(s) 263, 2401
TspRI CASTG 6 cut(s) 914, 1698, 2004, 2038, 2149, 2230
Tth111I GACNNNGTC 1 cut(s) 733
Van91I CCANNNNNTGG 1 cut(s) 2557
VneI GTGCAC 2 cut(s) 335, 1890
VpaK11BI GGWCC 1 cut(s) 1400
VspI ATTAAT 2 cut(s) 2022, 2544
XapI RAATTY 3 cut(s) 13, 45, 215
XbaI TCTAGA 2 cut(s) 518, 719
XceI RCATGY 1 cut(s) 691
XhoI CTCGAG 1 cut(s) 2615
XmnI GAANNNNTTC 3 cut(s) 1608, 1678, 1784
ZrmI AGTACT 2 cut(s) 1276, 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.