pycom08g00530

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001376
Physical Location & Seq
Reverse (-)
457880 .. 461322
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00530.5

Sequence Viewer

Length: 2517 bp
ATGAACAGTCCTTTTCGTCAAAAGAAAGAAAAGATTCGGAAACCGTGGGAAAATGCTGTGATGGCGGTCTCACATTGCCAGCCTGTTGTCGATTTTTCTCAAAGAAGGGACTCAGAAAATGGTGGGGAAAGTGATGAAGAAACTCTTGAGCTACAAGTACCAGAGCAGCAGCAGCAGCAATCGTATCAAGAAGAAGAGGATTATCAAATGGCAACGAGGGAAGGACAAGTTCTACTCAAGGTCGATGGCGGTGATGCTTCTTCCAGCAAAGAAACAGAGACTGCACCAAATATCGACGGAGAAGCAGCTCCAAGGTCGCCAATAATTCACGGCTCCCCAGAGAACCCTACTGAATCAGTCCGACGGAGAAGCAAAGATCTGCCCGGAACCTCCTCCAGCCCGGCCGGAGCTCCCGAGATTGTCCGATGCAGCCCCAACGCCTCCCCCCGAAGCTCCTGGAAACCGCCGGTGAGCAAGACTAAGTCCAGGCTGCTCGACCCACCACCTGAGGAAGCGTGTCTGAAATCGGACAGAGCTGTGGGCTCCGGACGAACTCCGCGGAGAGATGACGACGCTTCGGCTTTAGATGACGACGACCTCGAAGACATTCCAGATGAGTATAAGAAGATCAAGTTCGATGCCCTCACATTGGTTCAGTGGGTGAGTCTTGTTATTGTTATAGCTGCTTTAGTTTGCAATCTATTTATTCCATTAATAAAGAAGAAAACTTTGTGGAATCTTCCGCTGTGGAAGTGGGAAATTCTGGTTCTGGCATTGATTTGTGGTCGTTTGGTTTCGGGTTGGGGAATTCGGGTGATTGTGTTCTTTGTAGAGCGAAATTTCTTGTTGCGAAAACGGGTTTTGTATTTCGTATACGGTTTAAGAAGATCTGTTCAGAATTGTCTGTGGTTGGGATTGGTTTTGTTGGTATGGCGCTTGATTTTTGATAAGAAGATGGAGCAGAAGGCCAAGCATAGGATTTTGCCTTATGTGACCAAGATTTTGGTATGTTTTCTGGTTGGGACATTGATTTGGCTCCTGAAGACAATACTTGTTAAGGTTCTTGCCTTATATTTCCATGTCAACGCTTTCTTCGACCGGATCCAGGAGGTTTTGTTCATTCAGAATGTGTTTGAGACGCTATCTGGTCCCCCTATGTTTGAAAGGCAACACACACGGGAAGAGGAAAAGGCCGCGGCAGCTGAGATTAGGGAGTTTCAGAAGGCCGGGGTAGCTATGCCTCGTGATCTCAGGGCAACTCTTTTTCCGAGGACAAGGAGTGGGAGAGTTGTCGGGAGTGGAAGCCATAATAGCCCCAGAGTAGGGAAGAGTCCTGCGGTTTCTAGGCCAATGTCTGAGAGCCAAGATGAGGAGATCCCGGTAGATCACTTGCAGAAGCTAAATCAGAAGAATGTGTCGGCCTGGAATATGAGGAGGATGGTAAACATCATCCGGCACGGGTCCTTAACCACTCTAGATGAACATATACTGAACTCCAATGTGGGAGATGAGTCTTCTTTGAAGATTACAACTGAATGTCAAGCAAAAGAGGCAGCCAGGAAGATATTCATGAGAGTAGCCAAGCCAGGGTCCCCACACATTTACCTGGAGGATTTGATGCGGTTTATGGATAAAGATGAAGCTTTGAAGACGCTTCAACTCTTTGGTGCAGCAAGTGAACATGACAAAATCGGGAAGACCGCTCTTAAAAATTGGGTGGTCAATGCATTTAAAGAAAGAAGAGCTCTAGCATTGTCTTTGAATGATACAAAAACAGCTGTGGACGAACTCCATAAAATTATAAATGTCCTTGTAGCTATTGTCATTGTGATAATCTGGCTTATTATTCTTGGGGTTCATGTCAGCCACTACCTTCTCTTAATAAGCTCCCAGCTTCTTTTGGTTGCATTCGTCTTTGGGAACACATGTAAGACGGTTTTTGAAGCAATCATCTTTCTGTTTGTAAGGCATCCGTTTGATGTGGGTGATCGCTGTGAAGTGGATGGAGTCCAGATGGTAGTTGACGAGATGAATATATTAACAACAGTCTTTCTGAAGTATGATAACCAGAAGATCATATACCCTAACAGTGTTCTTGCGACCAAGGGAATTGCTAATTATCATCGTAGTCCTGACACGGTTGAATTTATTGATTTCTGTGTTCATATCGCAACTCCTATGGATAAGGTGGCCACTATGAAAGAAAGACTACAGAAGTATATGAAAAGCAGGAAGGATCACTGGCATGATGATCCGTTGCTGGTCACAAGGGATGTGCTGGATTTGAACAAACTGATGATATCGGTATGGCCGACACACAAGATGAACTATCAGGACATAGAGGAGAGATGGAGGAGGAGAAGCCTTTTGGTCGAAGAGATGATCAAAGTGTTCAGAGAGCTTGATATTGAGTACCGGTTACTGCCTATTGACATCAACGTCCGAGCCATGCCAAGTTTAACCTCCAACAGACTTCCTTCAAATTGGACAACCAGTGCCCCGAAGCCAGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0016020 GO:0044464 GO:0051179 GO:0051234 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel

Protein Analysis

839

Amino Acids

95.71

Weight (kDa)

8.77

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1802
AasI GACNNNNNNGTC 1 cut(s) 2438
AccBSI CCGCTC 1 cut(s) 1703
AccI GTMKAC 1 cut(s) 873
AccII CGCG 2 cut(s) 559, 1196
AccIII TCCGGA 1 cut(s) 545
AclWI GGATC 5 cut(s) 1096, 1109, 1369, 2244, 2246
AcoI YGGCCR 3 cut(s) 402, 2190, 2309
AcsI RAATTY 4 cut(s) 759, 807, 838, 2144
AcuI CTGAAG 2 cut(s) 1061, 2075
AfaI GTAC 2 cut(s) 159, 2414
AfiI CCNNNNNNNGG 7 cut(s) 649, 975, 1105, 1322, 1323, 1369, 1587
AflIII ACRYGT 1 cut(s) 1925
AgeI ACCGGT 1 cut(s) 2415
AgsI TTSAA 9 cut(s) 1163, 1522, 1648, 1658, 1762, 1943, 2144, 2287, 2481
AjnI CCWGG 7 cut(s) 455, 485, 1104, 1421, 1556, 1585, 1605
AloI GAACNNNNNNTCC 2 cut(s) 1100, 1132
Alw21I GWGCWC 2 cut(s) 412, 1747
Alw26I GTCTC 3 cut(s) 73, 272, 1130
AlwI GGATC 5 cut(s) 1096, 1109, 1369, 2244, 2246
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 1 cut(s) 413
Aor13HI TCCGGA 1 cut(s) 545
AoxI GGCC 8 cut(s) 402, 966, 1191, 1224, 1346, 1419, 2190, 2309
ApoI RAATTY 4 cut(s) 759, 807, 838, 2144
ArsI GACNNNNNNTTYG 2 cut(s) 985, 1017
AseI ATTAAT 1 cut(s) 713
AsiGI ACCGGT 1 cut(s) 2415
Asp700I GAANNNNTTC 3 cut(s) 33, 606, 1565
AspLEI GCGC 1 cut(s) 936
AspS9I GGNCC 3 cut(s) 1148, 1461, 1590
AsuC2I CCSGG 4 cut(s) 384, 401, 1228, 1379
AsuHPI GGTGA 5 cut(s) 263, 481, 673, 826, 1997
AvaI CYCGRG 1 cut(s) 413
AvaII GGWCC 3 cut(s) 1148, 1461, 1590
AxyI CCTNAGG 1 cut(s) 507
BaeGI GKGCMC 1 cut(s) 2500
BalI TGGCCA 1 cut(s) 2192
BamHI GGATCC 1 cut(s) 1101
BanII GRGCYC 3 cut(s) 412, 545, 1747
BarI GAAGNNNNNNTAC 2 cut(s) 2063, 2095
BauI CACGAG 1 cut(s) 1242
BbsI GAAGAC 5 cut(s) 609, 1049, 1506, 1655, 1703
Bbv12I GWGCWC 2 cut(s) 412, 1747
BccI CCATC 7 cut(s) 55, 239, 949, 1432, 1997, 2008, 2343
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 7 cut(s) 457, 487, 1106, 1423, 1558, 1587, 1607
BclI TGATCA 1 cut(s) 2382
BcnI CCSGG 4 cut(s) 384, 401, 1228, 1379
BcoDI GTCTC 3 cut(s) 73, 272, 1130
BfaI CTAG 3 cut(s) 1344, 1475, 1748
BfmI CTRYAG 1 cut(s) 2210
BfoI RGCGCY 1 cut(s) 937
BglII AGATCT 2 cut(s) 376, 887
Bme18I GGWCC 3 cut(s) 1148, 1461, 1590
BmeT110I CYCGRG 1 cut(s) 413
BmgT120I GGNCC 3 cut(s) 1148, 1461, 1590
BmiI GGNNCC 9 cut(s) 334, 388, 544, 1037, 1103, 1150, 1462, 1591, 1592
BmsI GCATC 5 cut(s) 244, 416, 628, 1608, 1978
BpiI GAAGAC 5 cut(s) 609, 1049, 1506, 1655, 1703
BpmI CTGGAG 2 cut(s) 379, 1628
BpuEI CTTGAG 2 cut(s) 167, 221
BpuMI CCSGG 4 cut(s) 384, 401, 1228, 1379
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 8 cut(s) 44, 311, 557, 1194, 1227, 1268, 1586, 2103
BsaWI WCCGGW 3 cut(s) 545, 1098, 2415
BsaXI ACNNNNNCTCC 2 cut(s) 1100, 1130
Bsc4I CCNNNNNNNGG 7 cut(s) 649, 975, 1105, 1322, 1323, 1369, 1587
Bse118I RCCGGY 2 cut(s) 466, 2415
Bse1I ACTGG 2 cut(s) 2246, 2493
Bse21I CCTNAGG 1 cut(s) 507
Bse3DI GCAATG 1 cut(s) 73
BseAI TCCGGA 1 cut(s) 545
BseBI CCWGG 7 cut(s) 457, 487, 1106, 1423, 1558, 1587, 1607
BseDI CCNNGG 8 cut(s) 44, 311, 557, 1194, 1227, 1268, 1586, 2103
BseGI GGATG 5 cut(s) 1443, 1449, 1969, 2008, 2278
BseLI CCNNNNNNNGG 7 cut(s) 649, 975, 1105, 1322, 1323, 1369, 1587
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 5 cut(s) 126, 498, 1194, 1264, 1347
BseNI ACTGG 2 cut(s) 2246, 2493
BseRI GAGGAG 6 cut(s) 382, 1385, 1447, 2357, 2368, 2371
BseSI GKGCMC 1 cut(s) 2500
BseX3I CGGCCG 1 cut(s) 402
BseYI CCCAGC 1 cut(s) 1890
BsgI GTGCAG 2 cut(s) 267, 1689
Bsh1236I CGCG 2 cut(s) 559, 1196
Bsh1285I CGRYCG 2 cut(s) 405, 1099
BshFI GGCC 8 cut(s) 404, 968, 1193, 1226, 1348, 1421, 2192, 2311
BshTI ACCGGT 1 cut(s) 2415
BsiEI CGRYCG 2 cut(s) 405, 1099
BsiHKAI GWGCWC 2 cut(s) 412, 1747
BsiHKCI CYCGRG 1 cut(s) 413
BslFI GGGAC 4 cut(s) 122, 1036, 1134, 1576
BslI CCNNNNNNNGG 7 cut(s) 649, 975, 1105, 1322, 1323, 1369, 1587
BsmAI GTCTC 3 cut(s) 73, 272, 1130
BsmBI CGTCTC 1 cut(s) 1130
BsmFI GGGAC 4 cut(s) 122, 1036, 1134, 1576
BsmI GAATGC 1 cut(s) 1907
BsnI GGCC 8 cut(s) 404, 968, 1193, 1226, 1348, 1421, 2192, 2311
Bso31I GGTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 413
Bsp1286I GDGCHC 4 cut(s) 412, 545, 1747, 2500
Bsp13I TCCGGA 1 cut(s) 545
BspANI GGCC 8 cut(s) 404, 968, 1193, 1226, 1348, 1421, 2192, 2311
BspCNI CTCAG 5 cut(s) 125, 499, 1195, 1263, 1348
BspEI TCCGGA 1 cut(s) 545
BspFNI CGCG 2 cut(s) 559, 1196
BspHI TCATGA 1 cut(s) 1569
BspLI GGNNCC 9 cut(s) 334, 388, 544, 1037, 1103, 1150, 1462, 1591, 1592
BspPI GGATC 5 cut(s) 1096, 1109, 1369, 2244, 2246
BspQI GCTCTTC 1 cut(s) 1735
BspTNI GGTCTC 1 cut(s) 73
BsrBI CCGCTC 1 cut(s) 1703
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 2 cut(s) 466, 2415
BsrI ACTGG 2 cut(s) 2246, 2493
BssAI RCCGGY 2 cut(s) 466, 2415
BssECI CCNNGG 8 cut(s) 44, 311, 557, 1194, 1227, 1268, 1586, 2103
BssNAI GTATAC 1 cut(s) 874
BssSI CACGAG 1 cut(s) 1242
BssT1I CCWWGG 2 cut(s) 311, 2103
Bst1107I GTATAC 1 cut(s) 874
Bst2BI CACGAG 1 cut(s) 1242
Bst2UI CCWGG 7 cut(s) 457, 487, 1106, 1423, 1558, 1587, 1607
Bst4CI ACNGT 7 cut(s) 8, 45, 878, 1936, 2047, 2090, 2140
Bst6I CTCTTC 5 cut(s) 189, 1176, 1322, 1735, 2370
BstC8I GCNNGC 2 cut(s) 80, 2508
BstDEI CTNAG 6 cut(s) 112, 480, 507, 1203, 1250, 1356
BstDSI CCRYGG 3 cut(s) 44, 557, 1194
BstF5I GGATG 5 cut(s) 1443, 1449, 1969, 2008, 2278
BstFNI CGCG 2 cut(s) 559, 1196
BstH2I RGCGCY 1 cut(s) 937
BstHHI GCGC 1 cut(s) 936
BstMAI GTCTC 3 cut(s) 73, 272, 1130
BstMCI CGRYCG 2 cut(s) 405, 1099
BstMWI GCNNNNNNNGC 7 cut(s) 62, 172, 175, 1199, 1232, 1311, 1550
BstNI CCWGG 7 cut(s) 457, 487, 1106, 1423, 1558, 1587, 1607
BstNSI RCATGY 1 cut(s) 1929
BstSFI CTRYAG 1 cut(s) 2210
BstSLI GKGCMC 1 cut(s) 2500
BstUI CGCG 2 cut(s) 559, 1196
BstV2I GAAGAC 5 cut(s) 609, 1049, 1506, 1655, 1703
BstX2I RGATCY 4 cut(s) 376, 887, 1101, 1374
BstXI CCANNNNNNTGG 1 cut(s) 1003
BstYI RGATCY 4 cut(s) 376, 887, 1101, 1374
BstZ17I GTATAC 1 cut(s) 874
BstZI CGGCCG 1 cut(s) 402
Bsu36I CCTNAGG 1 cut(s) 507
BsuRI GGCC 8 cut(s) 404, 968, 1193, 1226, 1348, 1421, 2192, 2311
BtgI CCRYGG 3 cut(s) 44, 557, 1194
BtsCI GGATG 5 cut(s) 1443, 1449, 1969, 2008, 2278
BtsIMutI CAGTG 4 cut(s) 662, 2095, 2239, 2500
Cac8I GCNNGC 2 cut(s) 80, 2508
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 1 cut(s) 1569
CfoI GCGC 1 cut(s) 936
Cfr10I RCCGGY 2 cut(s) 466, 2415
Cfr13I GGNCC 3 cut(s) 1148, 1461, 1590
Cfr42I CCGCGG 2 cut(s) 560, 1197
CseI GACGC 3 cut(s) 581, 1147, 1660
Csp6I GTAC 2 cut(s) 158, 2413
CspAI ACCGGT 1 cut(s) 2415
CviAII CATG 7 cut(s) 1079, 1570, 1682, 1859, 1926, 2246, 2449
CviQI GTAC 2 cut(s) 158, 2413
DdeI CTNAG 6 cut(s) 112, 480, 507, 1203, 1250, 1356
DraI TTTAAA 1 cut(s) 1732
DrdI GACNNNNNNGTC 1 cut(s) 2438
DseDI GACNNNNNNGTC 1 cut(s) 2438
EaeI YGGCCR 3 cut(s) 402, 2190, 2309
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 5 cut(s) 189, 1176, 1322, 1735, 2370
EarI CTCTTC 5 cut(s) 189, 1176, 1322, 1735, 2370
Ecl136II GAGCTC 2 cut(s) 410, 1745
EclXI CGGCCG 1 cut(s) 402
Eco130I CCWWGG 2 cut(s) 311, 2103
Eco24I GRGCYC 3 cut(s) 412, 545, 1747
Eco31I GGTCTC 1 cut(s) 73
Eco32I GATATC 1 cut(s) 2301
Eco47I GGWCC 3 cut(s) 1148, 1461, 1590
Eco52I CGGCCG 1 cut(s) 402
Eco53kI GAGCTC 2 cut(s) 410, 1745
Eco57I CTGAAG 2 cut(s) 1061, 2075
Eco81I CCTNAGG 1 cut(s) 507
Eco88I CYCGRG 1 cut(s) 413
EcoICRI GAGCTC 2 cut(s) 410, 1745
EcoO109I RGGNCCY 2 cut(s) 1461, 1590
EcoRI GAATTC 1 cut(s) 807
EcoRII CCWGG 7 cut(s) 455, 485, 1104, 1421, 1556, 1585, 1605
EcoRV GATATC 1 cut(s) 2301
EcoT14I CCWWGG 2 cut(s) 311, 2103
EcoT22I ATGCAT 1 cut(s) 1729
EcoT38I GRGCYC 3 cut(s) 412, 545, 1747
ErhI CCWWGG 2 cut(s) 311, 2103
Esp3I CGTCTC 1 cut(s) 1130
FaeI CATG 7 cut(s) 1082, 1573, 1685, 1862, 1929, 2249, 2452
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 4 cut(s) 122, 1036, 1134, 1576
FatI CATG 7 cut(s) 1078, 1569, 1681, 1858, 1925, 2245, 2448
FbaI TGATCA 1 cut(s) 2382
FblI GTMKAC 1 cut(s) 873
FokI GGATG 5 cut(s) 1436, 1450, 1956, 2015, 2285
FriOI GRGCYC 3 cut(s) 412, 545, 1747
FspBI CTAG 3 cut(s) 1344, 1475, 1748
GlaI GCGC 1 cut(s) 935
GsaI CCCAGC 1 cut(s) 1894
GsuI CTGGAG 2 cut(s) 379, 1628
HaeII RGCGCY 1 cut(s) 937
HaeIII GGCC 8 cut(s) 404, 968, 1193, 1226, 1348, 1421, 2192, 2311
HgaI GACGC 3 cut(s) 581, 1147, 1660
HhaI GCGC 1 cut(s) 936
Hin1II CATG 7 cut(s) 1082, 1573, 1685, 1862, 1929, 2249, 2452
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HincII GTYRAC 2 cut(s) 1084, 2023
HindII GTYRAC 2 cut(s) 1084, 2023
HindIII AAGCTT 1 cut(s) 1641
HinfI GANTC 8 cut(s) 34, 110, 353, 664, 736, 1330, 1511, 2007
HphI GGTGA 5 cut(s) 263, 481, 673, 826, 1997
Hpy166II GTNNAC 6 cut(s) 874, 1084, 1444, 1679, 1783, 2023
Hpy8I GTNNAC 6 cut(s) 874, 1084, 1444, 1679, 1783, 2023
Hpy99I CGWCG 4 cut(s) 299, 366, 575, 596
HpyAV CCTTC 7 cut(s) 99, 215, 958, 1216, 1883, 2227, 2487
HpyCH4III ACNGT 7 cut(s) 8, 45, 878, 1936, 2047, 2090, 2140
HpyCH4IV ACGT 1 cut(s) 2439
HpyCH4V TGCA 7 cut(s) 284, 429, 696, 1393, 1670, 1727, 1907
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 172, 175, 1199, 1232, 1311, 1550
HpyF3I CTNAG 6 cut(s) 112, 480, 507, 1203, 1250, 1356
HpySE526I ACGT 1 cut(s) 2439
Hsp92II CATG 7 cut(s) 1082, 1573, 1685, 1862, 1929, 2249, 2452
HspAI GCGC 1 cut(s) 934
KflI GGGWCCC 1 cut(s) 1590
Kpn2I TCCGGA 1 cut(s) 545
Ksp22I TGATCA 1 cut(s) 2382
KspI CCGCGG 2 cut(s) 560, 1197
LguI GCTCTTC 1 cut(s) 1735
LmnI GCTCC 9 cut(s) 313, 338, 407, 415, 458, 548, 958, 1041, 1892
LweI GCATC 5 cut(s) 244, 416, 628, 1608, 1978
MaeI CTAG 3 cut(s) 1344, 1475, 1748
MaeII ACGT 1 cut(s) 2439
MaeIII GTNAC 3 cut(s) 991, 2263, 2418
MbiI CCGCTC 1 cut(s) 1703
MflI RGATCY 4 cut(s) 376, 887, 1101, 1374
MhlI GDGCHC 4 cut(s) 412, 545, 1747, 2500
MlsI TGGCCA 1 cut(s) 2192
MluNI TGGCCA 1 cut(s) 2192
MlyI GAGTC 5 cut(s) 104, 673, 1339, 1520, 2016
MmeI TCCRAC 2 cut(s) 385, 2490
Mox20I TGGCCA 1 cut(s) 2192
Mph1103I ATGCAT 1 cut(s) 1729
MroI TCCGGA 1 cut(s) 545
MroXI GAANNNNTTC 3 cut(s) 33, 606, 1565
MscI TGGCCA 1 cut(s) 2192
MslI CAYNNNNRTG 1 cut(s) 2244
Msp20I TGGCCA 1 cut(s) 2192
MspA1I CMGCKG 5 cut(s) 559, 745, 1196, 1202, 1778
Mva1269I GAATGC 1 cut(s) 1907
MvaI CCWGG 7 cut(s) 457, 487, 1106, 1423, 1558, 1587, 1607
MvnI CGCG 2 cut(s) 559, 1196
MwoI GCNNNNNNNGC 7 cut(s) 62, 172, 175, 1199, 1232, 1311, 1550
NciI CCSGG 4 cut(s) 384, 401, 1228, 1379
NlaIII CATG 7 cut(s) 1082, 1573, 1685, 1862, 1929, 2249, 2452
NlaIV GGNNCC 9 cut(s) 334, 388, 544, 1037, 1103, 1150, 1462, 1591, 1592
NmuCI GTSAC 2 cut(s) 991, 2263
NsiI ATGCAT 1 cut(s) 1729
NspI RCATGY 1 cut(s) 1929
PagI TCATGA 1 cut(s) 1569
PciI ACATGT 1 cut(s) 1925
PciSI GCTCTTC 1 cut(s) 1735
PcsI WCGNNNNNNNCGW 3 cut(s) 597, 1092, 2309
PctI GAATGC 1 cut(s) 1907
PdmI GAANNNNTTC 3 cut(s) 33, 606, 1565
PfeI GAWTC 3 cut(s) 34, 353, 736
PflFI GACNNNGTC 1 cut(s) 481
PfoI TCCNGGA 2 cut(s) 455, 1104
PinAI ACCGGT 1 cut(s) 2415
PleI GAGTC 5 cut(s) 104, 672, 1338, 1519, 2015
PpsI GAGTC 5 cut(s) 104, 672, 1338, 1519, 2015
PpuMI RGGWCCY 2 cut(s) 1461, 1590
PscI ACATGT 1 cut(s) 1925
PshBI ATTAAT 1 cut(s) 713
PsiI TTATAA 1 cut(s) 1802
Psp124BI GAGCTC 2 cut(s) 412, 1747
Psp5II RGGWCCY 2 cut(s) 1461, 1590
Psp6I CCWGG 7 cut(s) 455, 485, 1104, 1421, 1556, 1585, 1605
PspFI CCCAGC 1 cut(s) 1890
PspGI CCWGG 7 cut(s) 455, 485, 1104, 1421, 1556, 1585, 1605
PspN4I GGNNCC 9 cut(s) 334, 388, 544, 1037, 1103, 1150, 1462, 1591, 1592
PspPI GGNCC 3 cut(s) 1148, 1461, 1590
PspPPI RGGWCCY 2 cut(s) 1461, 1590
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 4 cut(s) 376, 887, 1101, 1374
PsyI GACNNNGTC 1 cut(s) 481
PvuII CAGCTG 2 cut(s) 1202, 1778
RsaI GTAC 2 cut(s) 159, 2414
RsaNI GTAC 2 cut(s) 158, 2413
RseI CAYNNNNRTG 1 cut(s) 2244
SacI GAGCTC 2 cut(s) 412, 1747
SacII CCGCGG 2 cut(s) 560, 1197
SapI GCTCTTC 1 cut(s) 1735
Sau96I GGNCC 3 cut(s) 1148, 1461, 1590
SchI GAGTC 5 cut(s) 104, 673, 1339, 1520, 2016
SduI GDGCHC 4 cut(s) 412, 545, 1747, 2500
SfaNI GCATC 5 cut(s) 244, 416, 628, 1608, 1978
SfcI CTRYAG 1 cut(s) 2210
Sfr303I CCGCGG 2 cut(s) 560, 1197
SgrAI CRCCGGYG 1 cut(s) 466
SgrBI CCGCGG 2 cut(s) 560, 1197
SinI GGWCC 3 cut(s) 1148, 1461, 1590
SmiMI CAYNNNNRTG 1 cut(s) 2244
SmlI CTYRAG 2 cut(s) 146, 236
SmoI CTYRAG 2 cut(s) 146, 236
SspMI CTAG 3 cut(s) 1344, 1475, 1748
SstI GAGCTC 2 cut(s) 412, 1747
StyI CCWWGG 2 cut(s) 311, 2103
TaaI ACNGT 7 cut(s) 8, 45, 878, 1936, 2047, 2090, 2140
TaiI ACGT 1 cut(s) 2442
TaqI TCGA 8 cut(s) 90, 243, 294, 495, 600, 636, 1095, 2373
TauI GCSGC 2 cut(s) 1196, 1199
TfiI GAWTC 3 cut(s) 34, 353, 736
TscAI CASTG 4 cut(s) 662, 2095, 2246, 2500
TseFI GTSAC 2 cut(s) 991, 2263
Tsp45I GTSAC 2 cut(s) 991, 2263
TspGWI ACGGA 4 cut(s) 312, 379, 1962, 2244
TspRI CASTG 4 cut(s) 662, 2095, 2246, 2500
Tth111I GACNNNGTC 1 cut(s) 481
VpaK11BI GGWCC 3 cut(s) 1148, 1461, 1590
VspI ATTAAT 1 cut(s) 713
XapI RAATTY 4 cut(s) 759, 807, 838, 2144
XbaI TCTAGA 1 cut(s) 1474
XceI RCATGY 1 cut(s) 1929
XmiI GTMKAC 1 cut(s) 873
XmnI GAANNNNTTC 3 cut(s) 33, 606, 1565
XspI CTAG 3 cut(s) 1344, 1475, 1748
Zsp2I ATGCAT 1 cut(s) 1729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.