Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Forward (+)

33556410 .. 33563775

Gene Structure 7366 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g39430.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGGATTTTTCGCTGAAGAAATCGTTCAAATCCCATGGCTCGAGCAAACACATGAGGAAAATCTCTGCCGGATCAGCAGAAGACAACAGCCACGAGCAGCTCCCGATTCTAACCGACCACCACAGCCAGCCACTCCCCGCCGACCACCGCAAAGAGGTCATCGTCAAGATCGACGAGGGCAGTTCCTCAGATAACGTCAGGGAAGTGGACCCCGCCAAACATCACGGTGGTAGGATTTGGCGGGAATCGAGTATAGATTTCTGGTCGGAGGGCGACGGGAGAACTGTCGCCGCCGCCGGCAGCGGGGGCTTTCAGTTCACGCAGAGGGGGAAACAGATGACGCCGGAGGAGGATCCGCCGACGAGGCTGATAGGGCAGTTCCTCAACAAACAAAGAGGCTCCAACGGCGGCGGCGACGTGTCGTTGGACATGGATTTGGAGATGGACGAGCTACAACAACCACCGCTGGCGGAGTCGCCGATGACGTCGCGGGAGCTCAAGGTGTCGTTCCAGTCGCAGGCGCCGTCGGACCTTCCGGATACGCCGAACGATTCGGTTCGGCGGCGAAATAGAGACTCGGTGGTTGAGGAGGAACGACGTCGTAACAGTCGGTTGAGTAACGGCGGAGCCGACGTGGTTAAGTGCTCGTCAAACAAGTCGTTCCGGCGAGAAGTGTCGTTTCAGAACAACAATAAGTCTGATTTGTTGAGGATAAAGACTAGGTCTAGATTGATGGACCCGCCTGAAGGCTATGACGACGGTCGGGTTCCGAGATCGGGTCCGATGAAGTCCGGGATGTTGGGGAAAGGCGGCGACGACGACGACGACGACCCCTTTTTGGAGGAGGATTTACCTGATGAGTACAGGAGGGTAAAGTTTAATGCATTGACTTTGTTACAATGGCTTAGTTTGGTTTTGATCGTAGCTGCCTTGGTATGCACGCTATGTATTAGGGTTTTGAGGCAGAAGTACTTGTGGAAACTGATGCTGTGGAAATGGGAGGTGCTGATTTTGGTATCGATTTGCGGGAGGCTGGTTTCGGGTTGGGTGATAAAAATCATAGTGTTCTTCGTGGAAAGGAATTTCCTTCTGCGCAAGAGGGTGTTGTATTTTGTTTATGGGATCAGAAAGCCTGTGCAGAACTGTATTTGGCTTGGCTTTGTTTTGACGGCTTGGCATTTCATGCTAATCAGGAAGGTTGAGGAGGAAACCAATAACAAGACCCTAGAGTATGTGACTAAAGGCTTGCTTTGTCTCTTGATTGGGGTTTTGCTTTGGTTGGTGAAGACATTGATTGTGAAGGTGCTGGCTTCCTCTTTCCATGTTAGATCCTACTTTGATCGGATTCAGGACGCGCTTTTTAACCAGTATGTGATCCAAACTCTCTCGGGGCGGCCCTTGATTGAGGTACAGAATGCGGAGGATGAAGAAGAGAGGCTTAACGATGAGGTTAGGAAGCTGCAGAGTATTGCTGGGACAACTATGCCTCCTGACCTCAGATCAAATGCATTTCCATCTGCGAGAATTGGGAGGGCAGTGTCTGGGAGAAGCGGGATGTTGATTGCTGGAAGTGGGGGCCTTGCAGCCAAGAGTAGCAAGTTTCCGAGGCCAACAATGTCCAAGAAGTCAGAGGAGCAGGCTGGGATCACCATTGATCACTTGCATCGGCTCAATCCAAAGAATGTGTCAGCTTGGAATATGAAGAGGCTAATGAACGTTGTTAGAAAAGGGCATCTGACGACACTTGATGAGCAAATTCTGGATTCCACCGAGCCGGATGAGGGTGATACTCAGATCAGAAGTGAAGTTGAGGCAAAGGCTGCAGCTAAACAGATATTTAGCAATGTGGCTCGGCCTGGCTCCAAGTACATCTCCCTGGAGGATTTGTTGCGTTTCATGGGAGAAGAGGAGGCTTTGAAGACCATGAGTCTCATTGAAGAGTCCTCTGAGACAAGGAGAATCCGCAAAACTTCCTTGAAAAATTGGGTGGTAAACTGCTTTAGAGAACGGAGAGCACTTTCTTTGACACTGAATGACACCAAAACAGCGGTGAACAGACTTCGAATCGTGGTGGACGTATTAGTTGGCATTGTTATATTAGTCATATGGCTTCTTGTGCTGAATATTGTCACCAGCGAAAATCTTGTCTTTGCAACTTCGCAGCTTGTGGTTGTGGCATTTGTTTTTGGTAATACTTGCAAGACAGTATTTGAAGCAGTCATATTTTTATTCGTGATTCACCCCTTTGATGTGGGAGATCGATGTGAAATTGATGGAGTGCAGATGATCGTAGAAGAAATGAACATCTTGACTACAGTTTTTCTGAAGTATGACAACACCAAAATTGTGTACCCGAACAGTACCCTTTCAACTAAGCCCATCAGTAACTATTATCGCAGTCCGGACACACAAGATGCTATTGAGTTCTCTGTCCATATATCTACTCCGGCAGATAAGGTTGCTGCTATGAAGCAGAGAATGATAAGTTATATTGCAAATAAGAAGGAGCACTGGTATGCTGATCAAATGATCATCCTGAAGGATGTAGAAGAATTAAATAGGATGACGTTTGCATTGTGGTTGACACATAAAATGAACTTTCAAGATGTGGGAGAAAGGTGGGAAAGAAGAGCCCTTTTACTTGAAGAGATGGTAAGGATCTTCCAGGAGCTGGACATTCAGTACCGTCTGCTGCCTATTGACATAAATGTCCGCGGCATGCCTTCCACAACCACCACTGGTCTGCCTCCCTCAAATTTCTCAGCAACCACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

926

Amino Acids

105.12

Weight (kDa)

8.47

Isoelectric Point (pI)

48.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	741 - 798	7.9e-12	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2708
AatII	GACGTC	2 cut(s)	488, 601
Acc16I	TGCGCA	1 cut(s)	1094
AccB1I	GGYRCC	1 cut(s)	520
AccB7I	CCANNNNNTGG	1 cut(s)	2671
AccII	CGCG	3 cut(s)	490, 1355, 2715
AccIII	TCCGGA	2 cut(s)	535, 2401
AclI	AACGTT	1 cut(s)	1716
AclWI	GGATC	8 cut(s)	79, 347, 360, 1130, 1323, 1369, 1652, 2666
AcsI	RAATTY	3 cut(s)	1081, 1755, 2755
AcuI	CTGAAG	4 cut(s)	35, 765, 2345, 2558
AcyI	GRCGYC	4 cut(s)	341, 485, 521, 598
AfaI	GTAC	7 cut(s)	863, 971, 1410, 1868, 2351, 2362, 2684
AfiI	CCNNNNNNNGG	8 cut(s)	154, 303, 517, 746, 776, 838, 1781, 2671
AflIII	ACRYGT	1 cut(s)	417
AgsI	TTSAA	8 cut(s)	28, 1918, 1937, 1978, 2213, 2370, 2603, 2645
AhdI	GACNNNNNGTC	1 cut(s)	1926
AjiI	CACGTC	2 cut(s)	418, 634
AjnI	CCWGG	3 cut(s)	1855, 1875, 2664
Alw21I	GWGCWC	4 cut(s)	498, 647, 2017, 2511
Alw26I	GTCTC	4 cut(s)	567, 1259, 1934, 1943
AlwI	GGATC	8 cut(s)	79, 347, 360, 1130, 1323, 1369, 1652, 2666
AlwNI	CAGNNNCTG	2 cut(s)	1541, 2671
Ama87I	CYCGRG	2 cut(s)	40, 1387
Aor13HI	TCCGGA	2 cut(s)	535, 2401
AoxI	GGCC	4 cut(s)	1394, 1576, 1607, 1853
ApoI	RAATTY	3 cut(s)	1081, 1755, 2755
ArsI	GACNNNNNNTTYG	4 cut(s)	1670, 1702, 2130, 2162
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	3 cut(s)	523, 1095, 1357
AspS9I	GGNCC	6 cut(s)	208, 529, 736, 779, 1395, 1576
AsuC2I	CCSGG	1 cut(s)	793
AsuHPI	GGTGA	7 cut(s)	1060, 1294, 1639, 1796, 2062, 2122, 2231
AsuII	TTCGAA	1 cut(s)	2062
AvaI	CYCGRG	2 cut(s)	40, 1387
AvaII	GGWCC	4 cut(s)	208, 529, 736, 779
BamHI	GGATCC	1 cut(s)	352
BanI	GGYRCC	1 cut(s)	520
BanII	GRGCYC	2 cut(s)	498, 2635
BauI	CACGAG	1 cut(s)	92
BbsI	GAAGAC	3 cut(s)	87, 1292, 1925
Bbv12I	GWGCWC	4 cut(s)	498, 647, 2017, 2511
BccI	CCATC	6 cut(s)	436, 727, 1522, 2267, 2387, 2644
BceAI	ACGGC	4 cut(s)	421, 508, 637, 1185
BciT130I	CCWGG	3 cut(s)	1857, 1877, 2666
BciVI	GTATCC	1 cut(s)	532
BclI	TGATCA	3 cut(s)	1654, 2521, 2529
BcnI	CCSGG	1 cut(s)	793
BcoDI	GTCTC	4 cut(s)	567, 1259, 1934, 1943
BfaI	CTAG	3 cut(s)	720, 726, 1226
BfmI	CTRYAG	3 cut(s)	1460, 1821, 2313
BfoI	RGCGCY	1 cut(s)	524
BfuI	GTATCC	1 cut(s)	532
BglI	GCCNNNNNGGC	1 cut(s)	364
BmcAI	AGTACT	1 cut(s)	971
Bme1390I	CCNGG	4 cut(s)	793, 1857, 1877, 2666
Bme18I	GGWCC	4 cut(s)	208, 529, 736, 779
BmeRI	GACNNNNNGTC	1 cut(s)	1926
BmeT110I	CYCGRG	2 cut(s)	40, 1387
BmgBI	CACGTC	2 cut(s)	418, 634
BmgT120I	GGNCC	6 cut(s)	208, 529, 736, 779, 1395, 1576
BmrFI	CCNGG	4 cut(s)	793, 1857, 1877, 2666
BmsI	GCATC	4 cut(s)	975, 1672, 1741, 2404
BoxI	GACNNNNGTC	1 cut(s)	759
BpiI	GAAGAC	3 cut(s)	87, 1292, 1925
BpmI	CTGGAG	1 cut(s)	1898
Bpu14I	TTCGAA	1 cut(s)	2062
BpuEI	CTTGAG	1 cut(s)	482
BpuMI	CCSGG	1 cut(s)	793
Bsa29I	ATCGAT	2 cut(s)	1019, 2260
BsaBI	GATNNNNATC	1 cut(s)	1055
BsaHI	GRCGYC	4 cut(s)	341, 485, 521, 598
BsaJI	CCNNGG	5 cut(s)	34, 930, 1604, 1875, 2713
BsaWI	WCCGGW	2 cut(s)	535, 2401
BsaXI	ACNNNNNCTCC	8 cut(s)	618, 648, 1471, 1501, 1871, 1901, 2498, 2528
Bsc4I	CCNNNNNNNGG	8 cut(s)	154, 303, 517, 746, 776, 838, 1781, 2671
Bse118I	RCCGGY	1 cut(s)	296
Bse1I	ACTGG	4 cut(s)	511, 1366, 2516, 2743
Bse3DI	GCAATG	1 cut(s)	1849
Bse8I	GATNNNNATC	1 cut(s)	1055
BseAI	TCCGGA	2 cut(s)	535, 2401
BseBI	CCWGG	3 cut(s)	1857, 1877, 2666
BseCI	ATCGAT	2 cut(s)	1019, 2260
BseDI	CCNNGG	5 cut(s)	34, 930, 1604, 1875, 2713
BseGI	GGATG	7 cut(s)	801, 1429, 1560, 1783, 2532, 2548, 2568
BseJI	GATNNNNATC	1 cut(s)	1055
BseLI	CCNNNNNNNGG	8 cut(s)	154, 303, 517, 746, 776, 838, 1781, 2671
BseMI	GCAATG	1 cut(s)	1849
BseMII	CTCAG	5 cut(s)	201, 1510, 1805, 1938, 2775
BseNI	ACTGG	4 cut(s)	511, 1366, 2516, 2743
BseRI	GAGGAG	6 cut(s)	362, 602, 857, 1217, 1646, 1922
BseYI	CCCAGC	2 cut(s)	1472, 1640
BsgI	GTGCAG	2 cut(s)	1157, 2300
Bsh1236I	CGCG	3 cut(s)	490, 1355, 2715
Bsh1285I	CGRYCG	1 cut(s)	763
BshFI	GGCC	4 cut(s)	1396, 1578, 1609, 1855
BshNI	GGYRCC	1 cut(s)	520
BshVI	ATCGAT	2 cut(s)	1019, 2260
BsiEI	CGRYCG	1 cut(s)	763
BsiHKAI	GWGCWC	4 cut(s)	498, 647, 2017, 2511
BsiHKCI	CYCGRG	2 cut(s)	40, 1387
BsiSI	CCGG	9 cut(s)	69, 297, 344, 536, 664, 792, 1775, 2402, 2447
BslFI	GGGAC	1 cut(s)	1489
BslI	CCNNNNNNNGG	8 cut(s)	154, 303, 517, 746, 776, 838, 1781, 2671
BsmAI	GTCTC	4 cut(s)	567, 1259, 1934, 1943
BsmFI	GGGAC	1 cut(s)	1489
BsmI	GAATGC	1 cut(s)	1420
BsnI	GGCC	4 cut(s)	1396, 1578, 1609, 1855
BsoBI	CYCGRG	2 cut(s)	40, 1387
Bsp119I	TTCGAA	1 cut(s)	2062
Bsp1286I	GDGCHC	5 cut(s)	498, 647, 2017, 2511, 2635
Bsp13I	TCCGGA	2 cut(s)	535, 2401
Bsp19I	CCATGG	1 cut(s)	34
BspANI	GGCC	4 cut(s)	1396, 1578, 1609, 1855
BspCNI	CTCAG	5 cut(s)	200, 1509, 1804, 1939, 2774
BspDI	ATCGAT	2 cut(s)	1019, 2260
BspEI	TCCGGA	2 cut(s)	535, 2401
BspFNI	CGCG	3 cut(s)	490, 1355, 2715
BspMAI	CTGCAG	2 cut(s)	1464, 1825
BspPI	GGATC	8 cut(s)	79, 347, 360, 1130, 1323, 1369, 1652, 2666
BspQI	GCTCTTC	1 cut(s)	2623
BspT104I	TTCGAA	1 cut(s)	2062
BspT107I	GGYRCC	1 cut(s)	520
BsrDI	GCAATG	1 cut(s)	1849
BsrFI	RCCGGY	1 cut(s)	296
BsrI	ACTGG	4 cut(s)	511, 1366, 2516, 2743
BssAI	RCCGGY	1 cut(s)	296
BssECI	CCNNGG	5 cut(s)	34, 930, 1604, 1875, 2713
BssNI	GRCGYC	4 cut(s)	341, 485, 521, 598
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	2 cut(s)	34, 930
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	3 cut(s)	1857, 1877, 2666
Bst4CI	ACNGT	9 cut(s)	227, 286, 608, 761, 1145, 2206, 2317, 2360, 2687
Bst6I	CTCTTC	6 cut(s)	1425, 1697, 1899, 1932, 2623, 2640
BstACI	GRCGYC	4 cut(s)	341, 485, 521, 598
BstAPI	GCANNNNNTGC	2 cut(s)	1183, 1820
BstBI	TTCGAA	1 cut(s)	2062
BstC8I	GCNNGC	9 cut(s)	128, 298, 468, 519, 941, 1247, 1308, 1638, 2720
BstDEI	CTNAG	7 cut(s)	187, 905, 1496, 1791, 1947, 2373, 2761
BstDSI	CCRYGG	2 cut(s)	34, 2713
BstF5I	GGATG	7 cut(s)	801, 1429, 1560, 1783, 2532, 2548, 2568
BstFNI	CGCG	3 cut(s)	490, 1355, 2715
BstH2I	RGCGCY	1 cut(s)	524
BstHHI	GCGC	3 cut(s)	523, 1095, 1357
BstMAI	GTCTC	4 cut(s)	567, 1259, 1934, 1943
BstMCI	CGRYCG	1 cut(s)	763
BstMWI	GCNNNNNNNGC	8 cut(s)	74, 306, 364, 373, 405, 1183, 1820, 2116
BstNI	CCWGG	3 cut(s)	1857, 1877, 2666
BstNSI	RCATGY	1 cut(s)	2722
BstPAI	GACNNNNGTC	1 cut(s)	759
BstSCI	CCNGG	4 cut(s)	791, 1855, 1875, 2664
BstSFI	CTRYAG	3 cut(s)	1460, 1821, 2313
BstUI	CGCG	3 cut(s)	490, 1355, 2715
BstV2I	GAAGAC	3 cut(s)	87, 1292, 1925
BstX2I	RGATCY	3 cut(s)	352, 1328, 2658
BstYI	RGATCY	3 cut(s)	352, 1328, 2658
Bsu15I	ATCGAT	2 cut(s)	1019, 2260
BsuI	GTATCC	1 cut(s)	532
BsuRI	GGCC	4 cut(s)	1396, 1578, 1609, 1855
BsuTUI	ATCGAT	2 cut(s)	1019, 2260
BtgI	CCRYGG	2 cut(s)	34, 2713
BtrI	CACGTC	2 cut(s)	418, 634
BtsCI	GGATG	7 cut(s)	801, 1429, 1560, 1783, 2532, 2548, 2568
BtsI	GCAGTG	1 cut(s)	1542
BtsIMutI	CAGTG	4 cut(s)	1542, 2027, 2509, 2736
Cac8I	GCNNGC	9 cut(s)	128, 298, 468, 519, 941, 1247, 1308, 1638, 2720
CaiI	CAGNNNCTG	2 cut(s)	1541, 2671
CfoI	GCGC	3 cut(s)	523, 1095, 1357
Cfr10I	RCCGGY	1 cut(s)	296
Cfr13I	GGNCC	6 cut(s)	208, 529, 736, 779, 1395, 1576
Cfr42I	CCGCGG	1 cut(s)	2716
ClaI	ATCGAT	2 cut(s)	1019, 2260
CseI	GACGC	2 cut(s)	349, 1361
Csp6I	GTAC	7 cut(s)	862, 970, 1409, 1867, 2350, 2361, 2683
CviAII	CATG	8 cut(s)	35, 52, 430, 1183, 1322, 1897, 1924, 2719
CviQI	GTAC	7 cut(s)	862, 970, 1409, 1867, 2350, 2361, 2683
DdeI	CTNAG	7 cut(s)	187, 905, 1496, 1791, 1947, 2373, 2761
DinI	GGCGCC	1 cut(s)	522
DrdI	GACNNNNNNGTC	1 cut(s)	2708
DriI	GACNNNNNGTC	1 cut(s)	1926
DseDI	GACNNNNNNGTC	1 cut(s)	2708
Eam1104I	CTCTTC	6 cut(s)	1425, 1697, 1899, 1932, 2623, 2640
Eam1105I	GACNNNNNGTC	1 cut(s)	1926
EarI	CTCTTC	6 cut(s)	1425, 1697, 1899, 1932, 2623, 2640
EciI	GGCGGA	3 cut(s)	345, 485, 639
Ecl136II	GAGCTC	1 cut(s)	496
Eco130I	CCWWGG	2 cut(s)	34, 930
Eco24I	GRGCYC	2 cut(s)	498, 2635
Eco47I	GGWCC	4 cut(s)	208, 529, 736, 779
Eco53kI	GAGCTC	1 cut(s)	496
Eco57I	CTGAAG	4 cut(s)	35, 765, 2345, 2558
Eco88I	CYCGRG	2 cut(s)	40, 1387
EcoICRI	GAGCTC	1 cut(s)	496
EcoO109I	RGGNCCY	1 cut(s)	1576
EcoRII	CCWGG	3 cut(s)	1855, 1875, 2664
EcoT14I	CCWWGG	2 cut(s)	34, 930
EcoT22I	ATGCAT	2 cut(s)	886, 1510
EcoT38I	GRGCYC	2 cut(s)	498, 2635
EgeI	GGCGCC	1 cut(s)	522
EheI	GGCGCC	1 cut(s)	522
ErhI	CCWWGG	2 cut(s)	34, 930
FaeI	CATG	8 cut(s)	38, 55, 433, 1186, 1325, 1900, 1927, 2722
FalI	AAGNNNNNCTT	4 cut(s)	1233, 1265, 1422, 1454
FaqI	GGGAC	1 cut(s)	1489
FatI	CATG	8 cut(s)	34, 51, 429, 1182, 1321, 1896, 1923, 2718
FauI	CCCGC	8 cut(s)	145, 220, 234, 296, 483, 747, 1019, 1544
FauNDI	CATATG	1 cut(s)	2105
FbaI	TGATCA	3 cut(s)	1654, 2521, 2529
FokI	GGATG	7 cut(s)	808, 1436, 1567, 1790, 2519, 2555, 2575
FriOI	GRGCYC	2 cut(s)	498, 2635
FspBI	CTAG	3 cut(s)	720, 726, 1226
FspI	TGCGCA	1 cut(s)	1094
GlaI	GCGC	3 cut(s)	522, 1094, 1356
GsaI	CCCAGC	2 cut(s)	1476, 1644
GsuI	CTGGAG	1 cut(s)	1898
HaeII	RGCGCY	1 cut(s)	524
HaeIII	GGCC	4 cut(s)	1396, 1578, 1609, 1855
HapII	CCGG	9 cut(s)	69, 297, 344, 536, 664, 792, 1775, 2402, 2447
HgaI	GACGC	2 cut(s)	349, 1361
HhaI	GCGC	3 cut(s)	523, 1095, 1357
Hin1I	GRCGYC	4 cut(s)	341, 485, 521, 598
Hin1II	CATG	8 cut(s)	38, 55, 433, 1186, 1325, 1900, 1927, 2722
Hin6I	GCGC	3 cut(s)	521, 1093, 1355
HinP1I	GCGC	3 cut(s)	521, 1093, 1355
HincII	GTYRAC	1 cut(s)	2583
HindII	GTYRAC	1 cut(s)	2583
HpaII	CCGG	9 cut(s)	69, 297, 344, 536, 664, 792, 1775, 2402, 2447
HphI	GGTGA	7 cut(s)	1060, 1294, 1639, 1796, 2062, 2122, 2231
Hpy166II	GTNNAC	7 cut(s)	208, 318, 1993, 2053, 2074, 2350, 2583
Hpy8I	GTNNAC	7 cut(s)	208, 318, 1993, 2053, 2074, 2350, 2583
HpyAV	CCTTC	8 cut(s)	542, 740, 1097, 1189, 1294, 2497, 2533, 2733
HpyCH4III	ACNGT	9 cut(s)	227, 286, 608, 761, 1145, 2206, 2317, 2360, 2687
HpyCH4IV	ACGT	8 cut(s)	195, 417, 485, 598, 633, 1716, 2076, 2567
HpyF10VI	GCNNNNNNNGC	8 cut(s)	74, 306, 364, 373, 405, 1183, 1820, 2116
HpyF3I	CTNAG	7 cut(s)	187, 905, 1496, 1791, 1947, 2373, 2761
HpySE526I	ACGT	8 cut(s)	195, 417, 485, 598, 633, 1716, 2076, 2567
Hsp92I	GRCGYC	4 cut(s)	341, 485, 521, 598
Hsp92II	CATG	8 cut(s)	38, 55, 433, 1186, 1325, 1900, 1927, 2722
HspAI	GCGC	3 cut(s)	521, 1093, 1355
KasI	GGCGCC	1 cut(s)	520
Kpn2I	TCCGGA	2 cut(s)	535, 2401
KroI	GCCGGC	1 cut(s)	296
KroNI	GCCGGC	1 cut(s)	298
Ksp22I	TGATCA	3 cut(s)	1654, 2521, 2529
KspI	CCGCGG	1 cut(s)	2716
LguI	GCTCTTC	1 cut(s)	2623
LmnI	GCTCC	8 cut(s)	105, 404, 493, 626, 1633, 1865, 2506, 2668
LweI	GCATC	4 cut(s)	975, 1672, 1741, 2404
MaeI	CTAG	3 cut(s)	720, 726, 1226
MaeII	ACGT	8 cut(s)	195, 417, 485, 598, 633, 1716, 2076, 2567
MaeIII	GTNAC	6 cut(s)	602, 617, 894, 1234, 2128, 2384
MflI	RGATCY	3 cut(s)	352, 1328, 2658
MhlI	GDGCHC	5 cut(s)	498, 647, 2017, 2511, 2635
MluCI	AATT	8 cut(s)	1081, 1524, 1755, 1981, 2268, 2343, 2552, 2755
Mly113I	GGCGCC	1 cut(s)	521
MlyI	GAGTC	4 cut(s)	482, 569, 1936, 1949
MmeI	TCCRAC	4 cut(s)	246, 405, 426, 507
Mph1103I	ATGCAT	2 cut(s)	886, 1510
MroI	TCCGGA	2 cut(s)	535, 2401
MroNI	GCCGGC	1 cut(s)	296
MroXI	GAANNNNTTC	1 cut(s)	23
MseI	TTAA	5 cut(s)	639, 879, 1362, 1440, 2555
MslI	CAYNNNNRTG	2 cut(s)	225, 2514
MspA1I	CMGCKG	4 cut(s)	303, 466, 2048, 2715
MspI	CCGG	9 cut(s)	69, 297, 344, 536, 664, 792, 1775, 2402, 2447
MspR9I	CCNGG	4 cut(s)	793, 1857, 1877, 2666
Mva1269I	GAATGC	1 cut(s)	1420
MvaI	CCWGG	3 cut(s)	1857, 1877, 2666
MvnI	CGCG	3 cut(s)	490, 1355, 2715
MwoI	GCNNNNNNNGC	8 cut(s)	74, 306, 364, 373, 405, 1183, 1820, 2116
NaeI	GCCGGC	1 cut(s)	298
NarI	GGCGCC	1 cut(s)	521
NciI	CCSGG	1 cut(s)	793
NcoI	CCATGG	1 cut(s)	34
NdeI	CATATG	1 cut(s)	2105
NgoMIV	GCCGGC	1 cut(s)	296
NlaIII	CATG	8 cut(s)	38, 55, 433, 1186, 1325, 1900, 1927, 2722
NmeAIII	GCCGAG	1 cut(s)	1831
NmuCI	GTSAC	2 cut(s)	1234, 2128
NsbI	TGCGCA	1 cut(s)	1094
NsiI	ATGCAT	2 cut(s)	886, 1510
NspI	RCATGY	1 cut(s)	2722
NspV	TTCGAA	1 cut(s)	2062
PaeI	GCATGC	1 cut(s)	2722
PaeR7I	CTCGAG	1 cut(s)	40
PciSI	GCTCTTC	1 cut(s)	2623
PcsI	WCGNNNNNNNCGW	8 cut(s)	168, 411, 482, 521, 627, 822, 825, 2073
PctI	GAATGC	1 cut(s)	1420
PdiI	GCCGGC	1 cut(s)	298
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	8 cut(s)	106, 245, 551, 1345, 1763, 1959, 2064, 2236
PflFI	GACNNNGTC	1 cut(s)	721
PflMI	CCANNNNNTGG	1 cut(s)	2671
PfoI	TCCNGGA	2 cut(s)	791, 2664
PleI	GAGTC	4 cut(s)	481, 569, 1935, 1948
PluTI	GGCGCC	1 cut(s)	524
PpsI	GAGTC	4 cut(s)	481, 569, 1935, 1948
PshAI	GACNNNNGTC	1 cut(s)	759
Psp124BI	GAGCTC	1 cut(s)	498
Psp1406I	AACGTT	1 cut(s)	1716
Psp6I	CCWGG	3 cut(s)	1855, 1875, 2664
PspFI	CCCAGC	2 cut(s)	1472, 1640
PspGI	CCWGG	3 cut(s)	1855, 1875, 2664
PspPI	GGNCC	6 cut(s)	208, 529, 736, 779, 1395, 1576
PspXI	VCTCGAGB	1 cut(s)	40
PstI	CTGCAG	2 cut(s)	1464, 1825
PstNI	CAGNNNCTG	2 cut(s)	1541, 2671
PsuI	RGATCY	3 cut(s)	352, 1328, 2658
PsyI	GACNNNGTC	1 cut(s)	721
RsaI	GTAC	7 cut(s)	863, 971, 1410, 1868, 2351, 2362, 2684
RsaNI	GTAC	7 cut(s)	862, 970, 1409, 1867, 2350, 2361, 2683
RseI	CAYNNNNRTG	2 cut(s)	225, 2514
SacI	GAGCTC	1 cut(s)	498
SacII	CCGCGG	1 cut(s)	2716
SapI	GCTCTTC	1 cut(s)	2623
SaqAI	TTAA	5 cut(s)	639, 879, 1362, 1440, 2555
Sau96I	GGNCC	6 cut(s)	208, 529, 736, 779, 1395, 1576
ScaI	AGTACT	1 cut(s)	971
SchI	GAGTC	4 cut(s)	482, 569, 1936, 1949
ScrFI	CCNGG	4 cut(s)	793, 1857, 1877, 2666
SduI	GDGCHC	5 cut(s)	498, 647, 2017, 2511, 2635
SfaNI	GCATC	4 cut(s)	975, 1672, 1741, 2404
SfcI	CTRYAG	3 cut(s)	1460, 1821, 2313
SfoI	GGCGCC	1 cut(s)	522
Sfr274I	CTCGAG	1 cut(s)	40
Sfr303I	CCGCGG	1 cut(s)	2716
SfuI	TTCGAA	1 cut(s)	2062
SgrBI	CCGCGG	1 cut(s)	2716
SinI	GGWCC	4 cut(s)	208, 529, 736, 779
SlaI	CTCGAG	1 cut(s)	40
SmiMI	CAYNNNNRTG	2 cut(s)	225, 2514
SmlI	CTYRAG	2 cut(s)	40, 497
SmoI	CTYRAG	2 cut(s)	40, 497
SphI	GCATGC	1 cut(s)	2722
Sse9I	AATT	8 cut(s)	1081, 1524, 1755, 1981, 2268, 2343, 2552, 2755
SspDI	GGCGCC	1 cut(s)	520
SspI	AATATT	1 cut(s)	2125
SspMI	CTAG	3 cut(s)	720, 726, 1226
SstI	GAGCTC	1 cut(s)	498
StyD4I	CCNGG	4 cut(s)	791, 1855, 1875, 2664
StyI	CCWWGG	2 cut(s)	34, 930
TaaI	ACNGT	9 cut(s)	227, 286, 608, 761, 1145, 2206, 2317, 2360, 2687
TaiI	ACGT	8 cut(s)	198, 420, 488, 601, 636, 1719, 2079, 2570
TaqI	TCGA	6 cut(s)	41, 171, 248, 1019, 2062, 2260
TasI	AATT	8 cut(s)	1081, 1524, 1755, 1981, 2268, 2343, 2552, 2755
TatI	WGTACW	3 cut(s)	861, 969, 1866
TauI	GCSGC	8 cut(s)	293, 296, 411, 414, 565, 813, 1396, 2718
TfiI	GAWTC	8 cut(s)	106, 245, 551, 1345, 1763, 1959, 2064, 2236
Tru1I	TTAA	5 cut(s)	639, 879, 1362, 1440, 2555
Tru9I	TTAA	5 cut(s)	639, 879, 1362, 1440, 2555
TscAI	CASTG	4 cut(s)	1542, 2034, 2516, 2743
TseFI	GTSAC	2 cut(s)	1234, 2128
Tsp45I	GTSAC	2 cut(s)	1234, 2128
TspDTI	ATGAA	9 cut(s)	800, 1171, 1440, 1715, 1727, 1885, 2315, 2483, 2609
TspGWI	ACGGA	1 cut(s)	2023
TspRI	CASTG	4 cut(s)	1542, 2034, 2516, 2743
Tth111I	GACNNNGTC	1 cut(s)	721
Van91I	CCANNNNNTGG	1 cut(s)	2671
VpaK11BI	GGWCC	4 cut(s)	208, 529, 736, 779
XapI	RAATTY	3 cut(s)	1081, 1755, 2755
XbaI	TCTAGA	1 cut(s)	725
XceI	RCATGY	1 cut(s)	2722
XhoI	CTCGAG	1 cut(s)	40
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	3 cut(s)	720, 726, 1226
ZraI	GACGTC	2 cut(s)	486, 599
ZrmI	AGTACT	1 cut(s)	971
Zsp2I	ATGCAT	2 cut(s)	886, 1510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.