Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Reverse (-)

15725504 .. 15729343

Gene Structure 3840 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g12260.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2811 bp

ATGGAGTTCTCCATCAAGAAACCGTTTAAATCCCATGGCTCTGCCAAGCATATGAGGAAGATCTCCGCCGGAGCAGAAGACATCAGCCACGAGGAGCTCCCCATTCTTCTGGATCACGACCACCGCCACCACACCCAGCCGATGACGGACGTCGATTTGGCCGACCGCCGGGAAGTCATCGTCAAAATCGACGAAGGCGCAGATTCGTCCACAAGCACCAGCGACCCTGCCAAAAATGGCAGGAAGGTTTGGCGGCAGTCCAGCATCGATTTCTGGCACGGGGAAGGCAACGGCACTGGCAATGCCGAGAATTTTGATTTCGTGCAGCGTGGGAAGACGGATGAGGGGACTGGGGAGGATCCGCCGTCGAAGCTGATTGGGCAGTTCCTGCATAAGCAGCGAGCCTCCGGCGACATGACGCTGGATATGGATTTGGAGATGGAGGAGCTGCAGAGCGAGCGCGATTTGCCGCCCCTCGCCGAGTCGCCGAGTCCGAGGAACTCGCGCAACTCGAAGGAGCTCAAAGTCTCGTTCCAATCGCCGGCGCCGGACCTCACCGATACGCCGCAAGCCGAGTCCGTTCGGAGGCGATACAGGGAGTCCCCCGATGAGGAGCGGCGCCGCAGCGAGAGGTTGAGCAACGGACAGGACGACGTCGTCCGGTGCACTTCCAACGCCTCATTCCGCCGAGAGCCTTCGTTCTCGAACAAGTCCGATTTGTTGAGAATAAAGACCAAGTCCAGACTAATGGACCCGCCCGAAGAGCCGGATTTCAAGTCGGGCCGGATCCCGCGGTCGGGACAGATCCCGAAATCGGGTCAGATGAGGTCCGGGTTGCTGAGCCGCGGCGGCGACGACGACGACGACGACCCGTTTCTGGAGGAGGACGTGCCGCACGAGTACAAGAGGGCGAACTTCAACGCATTGACGCTGCTTCAGTGGGTAAGCTTGGTTTTGATCATTGGGGCGGTGGTTTGCACGCTGACGATTCCAGTTCTGAGGGAGAAGAGCTTGTGGAAACTGAAATTGTGGAAGTGGGAGGTCTTGATTTTGGTTCTGATATGCGGGAGATTGGTTTCGGGTTGGGGGATTAGGATCATAGTGTTTTTTGTGGAGAGGAATTTCCTTCTGCGGAAGAGAGTTTTGTATTTCGTGTATGGAGTCCGAAAGGCGGTGCAGAACTGCCTCTGGCTCGGCCTGGTTCTGATAGCATGGCATTTCATGTTTGATAAGAAGGTGGAAAGGGAGACCAATAGCGAGGTGGTCGCTTATGTGACGAAAATCTTGTTCTGTCTCTTGATTGGAGTGTTGCTGTGGCTGGTGAAGACGTTGATTGTGAAGGTGCTGGCTTCGTCATTCCATGTGAGGTCGTATTTTGATCGGATTCAGGACTCGCTTTTTAATCAGTATGTGATTGAGACGCTGTCCGGGCGGCCTTTGATTGAGATGCAGAATGCGGAGGAGGAGGACGATAGGCTGGCGGATGAGGTCAGGAAGCTGCAGAATGCCGGGGCTACTATGCCTCCTGACCTCAAGGCAAATGCATTCCCGTCCGCTAGGATTGGGAGGGTGATTGGGAGTGGTTCGATAAGGAGTGGGAGGGTGATTGCGGGTAGCGGGCTTATTGGGAAGAGCACCAAGTATTCTAAGCCACTTTCCAAGAAGGCAGAGGAGACCGGGATCACGATTGACCACTTGCATAAGCTCAATCCCAAGAATGTGTCTGCTTGGAATATGAAGAGGCTGATGAATATTGTTAGAAAAGGGCATCTTAAGACGCTTGATGAGCAGATTCTGGATGCCACTGGTGAGGATGAAGCTGATACTCAGATCAGGAGTGAGGTTGAGGCAAAGGCTGCTGCTAGAAAGATATTCCAGAATGTGGCTAGGCCTGGTTCCAAGTTCATATACCTGGAGGACTTGATGCGTTTCATGGAAGAAGACGAGGCTGTGAAGACCATGAGTCTTTTTGAAGGGGCATCTGAGCACAAGAGAATCAGCAAATCTGCCTTGAAAAATTGGGTGGTCAGTGCCTTCAGAGAACGGAGAGCACTTGCTCTGACGCTGAATGATACCAAAACAGCTGTGAATACACTTCATCGTGTGGTGAACATAATAGTTGCCATTGTTATAATTGTTATATGGCTTCTGGTTCTAGGAATTGCCACCAGCAAATTTCTCTTGTTTGTGAGTTCTCAGCTTGTGGTTGTGGCATTTGTCTTTGGTAACACCTGCAAGACGATATTTGAAGCAATCATATTTTTATTCGTGATGCATCCATTTGACGTGGGAGATCGTTGCGAAATAGACGGAGTTCAGATGGTAGTTGAAGAAATGAACATCTTGAGTACAGTTTTCCTAAGGTATGACAACACAAAAATCGTGTACCCAAACAGCATCCTCGCGACTTTGCCCATCCTTAACTACTATCGTAGTCCTGACACGGGAGATGCTATTGAGTTCTGCATCCATGTATCAACTCCGCCAGATAGGATTGTTTTAATGAGGCAAAGAATAATCAGTTACATCGAGAACAAGAAGGAGCACTGGTATCCAGCACCAATGATTATAACGAAGGATGTAGAAGAACTAAATAGGGTACGGTTTGCAATTTGGCCTCAACATAGAATGAACTTCCAGGACATAGGAGAAAGGTTTGTAAGGAGAGGCTATTTGGTCGAAGAGATGGTACGCATATTCCAGGAGCTTGACATCCAGTTCCGCCTTCTGCCTATTGACATAAACGTCCGAGCCATGCCTGGCATGACTGGCCAGGTTCCCTCTAATTACACGGCAACTAAAGCCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

937

Amino Acids

106.7

Weight (kDa)

8.36

Isoelectric Point (pI)

43.07

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2132, 2570
AarI	CACCTGC	1 cut(s)	2240
AasI	GACNNNNNNGTC	2 cut(s)	656, 2744
AatII	GACGTC	2 cut(s)	153, 657
Acc36I	ACCTGC	1 cut(s)	2240
AccB1I	GGYRCC	2 cut(s)	544, 618
AccB7I	CCANNNNNTGG	1 cut(s)	1882
AccBSI	CCGCTC	1 cut(s)	616
AccII	CGCG	5 cut(s)	462, 505, 793, 846, 2405
AclWI	GGATC	8 cut(s)	120, 353, 366, 781, 794, 799, 1103, 1688
AcoI	YGGCCR	2 cut(s)	159, 2770
AcsI	RAATTY	3 cut(s)	310, 1120, 2174
AcuI	CTGAAG	2 cut(s)	920, 2020
AcyI	GRCGYC	4 cut(s)	150, 545, 619, 654
AfaI	GTAC	5 cut(s)	902, 2350, 2387, 2601, 2691
AflII	CTTAAG	1 cut(s)	1772
AgsI	TTSAA	6 cut(s)	775, 919, 1973, 2014, 2249, 2330
AhdI	GACNNNNNGTC	1 cut(s)	1962
AjiI	CACGTC	2 cut(s)	889, 2287
AjnI	CCWGG	7 cut(s)	1197, 1891, 1911, 2637, 2700, 2758, 2772
AjuI	GAANNNNNNNTTGG	2 cut(s)	665, 697
Alw21I	GWGCWC	7 cut(s)	99, 522, 668, 1637, 1989, 2053, 2547
Alw26I	GTCTC	5 cut(s)	532, 1241, 1298, 1412, 1667
Alw44I	GTGCAC	1 cut(s)	664
AlwI	GGATC	8 cut(s)	120, 353, 366, 781, 794, 799, 1103, 1688
AlwNI	CAGNNNCTG	2 cut(s)	388, 1795
AoxI	GGCC	7 cut(s)	159, 781, 1195, 1433, 1889, 2615, 2770
ApaLI	GTGCAC	1 cut(s)	664
ApeKI	GCWGC	8 cut(s)	325, 397, 448, 624, 931, 1498, 1856, 1859
ApoI	RAATTY	3 cut(s)	310, 1120, 2174
AspLEI	GCGC	5 cut(s)	200, 462, 507, 547, 621
AspS9I	GGNCC	4 cut(s)	550, 751, 781, 828
AsuC2I	CCSGG	5 cut(s)	170, 832, 1429, 1510, 1678
AsuHPI	GGTGA	6 cut(s)	547, 1333, 1582, 1615, 1820, 2119
AvaII	GGWCC	3 cut(s)	550, 751, 828
AxyI	CCTNAGG	1 cut(s)	2360
BaeGI	GKGCMC	1 cut(s)	668
BalI	TGGCCA	1 cut(s)	2772
BamHI	GGATCC	2 cut(s)	358, 786
BanI	GGYRCC	2 cut(s)	544, 618
BanII	GRGCYC	2 cut(s)	99, 522
BarI	GAAGNNNNNNTAC	2 cut(s)	2673, 2705
BauI	CACGAG	2 cut(s)	89, 896
BbsI	GAAGAC	5 cut(s)	84, 341, 1331, 1947, 1961
Bbv12I	GWGCWC	7 cut(s)	99, 522, 668, 1637, 1989, 2053, 2547
BbvI	GCAGC	8 cut(s)	337, 409, 435, 636, 918, 1485, 1843, 1846
BccI	CCATC	5 cut(s)	20, 433, 2314, 2423, 2680
BceAI	ACGGC	3 cut(s)	307, 349, 2808
BciT130I	CCWGG	7 cut(s)	1199, 1893, 1913, 2639, 2702, 2760, 2774
BciVI	GTATCC	1 cut(s)	2562
BclI	TGATCA	1 cut(s)	957
BcnI	CCSGG	5 cut(s)	170, 832, 1429, 1510, 1678
BcoDI	GTCTC	5 cut(s)	532, 1241, 1298, 1412, 1667
BfaI	CTAG	4 cut(s)	1557, 1863, 1887, 2156
BfmI	CTRYAG	2 cut(s)	449, 1499
BfoI	RGCGCY	2 cut(s)	548, 622
BfrI	CTTAAG	1 cut(s)	1772
BfuAI	ACCTGC	1 cut(s)	2240
BfuI	GTATCC	1 cut(s)	2562
BglI	GCCNNNNNGGC	1 cut(s)	849
BglII	AGATCT	1 cut(s)	60
BlpI	GCTNAGC	1 cut(s)	839
Bme18I	GGWCC	3 cut(s)	550, 751, 828
BmeRI	GACNNNNNGTC	1 cut(s)	1962
BmgBI	CACGTC	2 cut(s)	889, 2287
BmgT120I	GGNCC	4 cut(s)	550, 751, 781, 828
BmiI	GGNNCC	7 cut(s)	360, 546, 620, 753, 788, 1897, 2778
BmrI	ACTGGG	1 cut(s)	360
BmuI	ACTGGG	1 cut(s)	360
BoxI	GACNNNNGTC	1 cut(s)	149
BpiI	GAAGAC	5 cut(s)	84, 341, 1331, 1947, 1961
BpmI	CTGGAG	2 cut(s)	899, 1934
Bpu1102I	GCTNAGC	1 cut(s)	839
BpuEI	CTTGAG	2 cut(s)	1517, 2365
BpuMI	CCSGG	5 cut(s)	170, 832, 1429, 1510, 1678
Bsa29I	ATCGAT	1 cut(s)	267
BsaBI	GATNNNNATC	1 cut(s)	1094
BsaHI	GRCGYC	4 cut(s)	150, 545, 619, 654
BsaI	GGTCTC	2 cut(s)	1241, 1667
BsaJI	CCNNGG	5 cut(s)	34, 494, 791, 844, 1509
BsaWI	WCCGGW	1 cut(s)	660
BsaXI	ACNNNNNCTCC	4 cut(s)	1507, 1537, 2534, 2564
Bse118I	RCCGGY	1 cut(s)	541
Bse1I	ACTGG	7 cut(s)	301, 355, 992, 1810, 2552, 2716, 2773
Bse21I	CCTNAGG	1 cut(s)	2360
Bse3DI	GCAATG	1 cut(s)	307
Bse8I	GATNNNNATC	1 cut(s)	1094
BseBI	CCWGG	7 cut(s)	1199, 1893, 1913, 2639, 2702, 2760, 2774
BseCI	ATCGAT	1 cut(s)	267
BseDI	CCNNGG	5 cut(s)	34, 494, 791, 844, 1509
BseJI	GATNNNNATC	1 cut(s)	1094
BseMI	GCAATG	1 cut(s)	307
BseMII	CTCAG	5 cut(s)	830, 989, 1841, 1974, 2210
BseNI	ACTGG	7 cut(s)	301, 355, 992, 1810, 2552, 2716, 2773
BseRI	GAGGAG	7 cut(s)	107, 458, 626, 896, 1475, 1478, 1685
BseSI	GKGCMC	1 cut(s)	668
BseXI	GCAGC	8 cut(s)	337, 409, 435, 636, 918, 1485, 1843, 1846
BseYI	CCCAGC	1 cut(s)	135
BsgI	GTGCAG	2 cut(s)	344, 1196
Bsh1236I	CGCG	5 cut(s)	462, 505, 793, 846, 2405
Bsh1285I	CGRYCG	2 cut(s)	166, 797
BshFI	GGCC	7 cut(s)	161, 783, 1197, 1435, 1891, 2617, 2772
BshNI	GGYRCC	2 cut(s)	544, 618
BshVI	ATCGAT	1 cut(s)	267
BsiEI	CGRYCG	2 cut(s)	166, 797
BsiHKAI	GWGCWC	7 cut(s)	99, 522, 668, 1637, 1989, 2053, 2547
BslFI	GGGAC	3 cut(s)	361, 586, 813
BsmAI	GTCTC	5 cut(s)	532, 1241, 1298, 1412, 1667
BsmBI	CGTCTC	1 cut(s)	1412
BsmFI	GGGAC	3 cut(s)	361, 586, 813
BsmI	GAATGC	3 cut(s)	1459, 1510, 1544
BsnI	GGCC	7 cut(s)	161, 783, 1197, 1435, 1891, 2617, 2772
Bso31I	GGTCTC	2 cut(s)	1241, 1667
Bsp1286I	GDGCHC	7 cut(s)	99, 522, 668, 1637, 1989, 2053, 2547
Bsp1720I	GCTNAGC	1 cut(s)	839
Bsp19I	CCATGG	1 cut(s)	34
Bsp68I	TCGCGA	1 cut(s)	2405
BspANI	GGCC	7 cut(s)	161, 783, 1197, 1435, 1891, 2617, 2772
BspCNI	CTCAG	5 cut(s)	831, 990, 1840, 1975, 2209
BspDI	ATCGAT	1 cut(s)	267
BspFNI	CGCG	5 cut(s)	462, 505, 793, 846, 2405
BspLI	GGNNCC	7 cut(s)	360, 546, 620, 753, 788, 1897, 2778
BspMAI	CTGCAG	2 cut(s)	453, 1503
BspMI	ACCTGC	1 cut(s)	2240
BspPI	GGATC	8 cut(s)	120, 353, 366, 781, 794, 799, 1103, 1688
BspQI	GCTCTTC	3 cut(s)	756, 1001, 1625
BspT107I	GGYRCC	2 cut(s)	544, 618
BspTI	CTTAAG	1 cut(s)	1772
BspTNI	GGTCTC	2 cut(s)	1241, 1667
BsrBI	CCGCTC	1 cut(s)	616
BsrDI	GCAATG	1 cut(s)	307
BsrFI	RCCGGY	1 cut(s)	541
BsrI	ACTGG	7 cut(s)	301, 355, 992, 1810, 2552, 2716, 2773
BssAI	RCCGGY	1 cut(s)	541
BssECI	CCNNGG	5 cut(s)	34, 494, 791, 844, 1509
BssNI	GRCGYC	4 cut(s)	150, 545, 619, 654
BssSI	CACGAG	2 cut(s)	89, 896
BssT1I	CCWWGG	1 cut(s)	34
Bst2BI	CACGAG	2 cut(s)	89, 896
Bst2UI	CCWGG	7 cut(s)	1199, 1893, 1913, 2639, 2702, 2760, 2774
Bst4CI	ACNGT	3 cut(s)	24, 2353, 2604
Bst6I	CTCTTC	6 cut(s)	756, 1001, 1130, 1625, 1733, 2676
BstACI	GRCGYC	4 cut(s)	150, 545, 619, 654
BstAFI	CTTAAG	1 cut(s)	1772
BstAPI	GCANNNNNTGC	2 cut(s)	388, 1856
BstC8I	GCNNGC	8 cut(s)	402, 458, 543, 570, 980, 1347, 1479, 1619
BstDEI	CTNAG	7 cut(s)	839, 998, 1647, 1827, 1983, 2196, 2360
BstDSI	CCRYGG	3 cut(s)	34, 791, 844
BstFNI	CGCG	5 cut(s)	462, 505, 793, 846, 2405
BstH2I	RGCGCY	2 cut(s)	548, 622
BstHHI	GCGC	5 cut(s)	200, 462, 507, 547, 621
BstMAI	GTCTC	5 cut(s)	532, 1241, 1298, 1412, 1667
BstMCI	CGRYCG	2 cut(s)	166, 797
BstNI	CCWGG	7 cut(s)	1199, 1893, 1913, 2639, 2702, 2760, 2774
BstPAI	GACNNNNGTC	1 cut(s)	149
BstSFI	CTRYAG	2 cut(s)	449, 1499
BstSLI	GKGCMC	1 cut(s)	668
BstUI	CGCG	5 cut(s)	462, 505, 793, 846, 2405
BstV1I	GCAGC	8 cut(s)	337, 409, 435, 636, 918, 1485, 1843, 1846
BstV2I	GAAGAC	5 cut(s)	84, 341, 1331, 1947, 1961
BstX2I	RGATCY	4 cut(s)	60, 358, 786, 804
BstXI	CCANNNNNNTGG	2 cut(s)	109, 748
BstYI	RGATCY	4 cut(s)	60, 358, 786, 804
Bsu15I	ATCGAT	1 cut(s)	267
Bsu36I	CCTNAGG	1 cut(s)	2360
BsuI	GTATCC	1 cut(s)	2562
BsuRI	GGCC	7 cut(s)	161, 783, 1197, 1435, 1891, 2617, 2772
BsuTUI	ATCGAT	1 cut(s)	267
BtgI	CCRYGG	3 cut(s)	34, 791, 844
BtrI	CACGTC	2 cut(s)	889, 2287
BtsIMutI	CAGTG	5 cut(s)	294, 944, 1803, 2035, 2545
BtuMI	TCGCGA	1 cut(s)	2405
BveI	ACCTGC	1 cut(s)	2240
Cac8I	GCNNGC	8 cut(s)	402, 458, 543, 570, 980, 1347, 1479, 1619
CaiI	CAGNNNCTG	2 cut(s)	388, 1795
CfoI	GCGC	5 cut(s)	200, 462, 507, 547, 621
Cfr10I	RCCGGY	1 cut(s)	541
Cfr13I	GGNCC	4 cut(s)	550, 751, 781, 828
Cfr42I	CCGCGG	2 cut(s)	794, 847
ClaI	ATCGAT	1 cut(s)	267
CseI	GACGC	5 cut(s)	427, 937, 1429, 1786, 2071
Csp6I	GTAC	5 cut(s)	901, 2349, 2386, 2600, 2690
CviQI	GTAC	5 cut(s)	901, 2349, 2386, 2600, 2690
DdeI	CTNAG	7 cut(s)	839, 998, 1647, 1827, 1983, 2196, 2360
DinI	GGCGCC	2 cut(s)	546, 620
DraI	TTTAAA	1 cut(s)	28
DrdI	GACNNNNNNGTC	2 cut(s)	656, 2744
DriI	GACNNNNNGTC	1 cut(s)	1962
DseDI	GACNNNNNNGTC	2 cut(s)	656, 2744
EaeI	YGGCCR	2 cut(s)	159, 2770
Eam1104I	CTCTTC	6 cut(s)	756, 1001, 1130, 1625, 1733, 2676
Eam1105I	GACNNNNNGTC	1 cut(s)	1962
EarI	CTCTTC	6 cut(s)	756, 1001, 1130, 1625, 1733, 2676
EciI	GGCGGA	6 cut(s)	55, 351, 674, 1496, 2472, 2711
Ecl136II	GAGCTC	2 cut(s)	97, 520
Eco130I	CCWWGG	1 cut(s)	34
Eco147I	AGGCCT	1 cut(s)	1891
Eco24I	GRGCYC	2 cut(s)	99, 522
Eco31I	GGTCTC	2 cut(s)	1241, 1667
Eco47I	GGWCC	3 cut(s)	550, 751, 828
Eco53kI	GAGCTC	2 cut(s)	97, 520
Eco57I	CTGAAG	2 cut(s)	920, 2020
Eco81I	CCTNAGG	1 cut(s)	2360
EcoICRI	GAGCTC	2 cut(s)	97, 520
EcoRII	CCWGG	7 cut(s)	1197, 1891, 1911, 2637, 2700, 2758, 2772
EcoT14I	CCWWGG	1 cut(s)	34
EcoT22I	ATGCAT	2 cut(s)	1546, 2277
EcoT38I	GRGCYC	2 cut(s)	99, 522
EgeI	GGCGCC	2 cut(s)	546, 620
EheI	GGCGCC	2 cut(s)	546, 620
ErhI	CCWWGG	1 cut(s)	34
Esp3I	CGTCTC	1 cut(s)	1412
FalI	AAGNNNNNCTT	2 cut(s)	1755, 1787
FaqI	GGGAC	3 cut(s)	361, 586, 813
FauI	CCCGC	5 cut(s)	762, 798, 1058, 1603, 1610
FauNDI	CATATG	1 cut(s)	51
FbaI	TGATCA	1 cut(s)	957
FriOI	GRGCYC	2 cut(s)	99, 522
FspBI	CTAG	4 cut(s)	1557, 1863, 1887, 2156
GlaI	GCGC	5 cut(s)	199, 461, 506, 546, 620
GsaI	CCCAGC	1 cut(s)	139
GsuI	CTGGAG	2 cut(s)	899, 1934
HaeII	RGCGCY	2 cut(s)	548, 622
HaeIII	GGCC	7 cut(s)	161, 783, 1197, 1435, 1891, 2617, 2772
HgaI	GACGC	5 cut(s)	427, 937, 1429, 1786, 2071
HhaI	GCGC	5 cut(s)	200, 462, 507, 547, 621
Hin1I	GRCGYC	4 cut(s)	150, 545, 619, 654
Hin6I	GCGC	5 cut(s)	198, 460, 505, 545, 619
HinP1I	GCGC	5 cut(s)	198, 460, 505, 545, 619
HindIII	AAGCTT	1 cut(s)	946
HphI	GGTGA	6 cut(s)	547, 1333, 1582, 1615, 1820, 2119
Hpy166II	GTNNAC	4 cut(s)	210, 666, 2110, 2386
Hpy8I	GTNNAC	4 cut(s)	210, 666, 2110, 2386
HpyCH4III	ACNGT	3 cut(s)	24, 2353, 2604
HpyCH4IV	ACGT	6 cut(s)	150, 654, 888, 1328, 2286, 2745
HpyF3I	CTNAG	7 cut(s)	839, 998, 1647, 1827, 1983, 2196, 2360
HpySE526I	ACGT	6 cut(s)	150, 654, 888, 1328, 2286, 2745
Hsp92I	GRCGYC	4 cut(s)	150, 545, 619, 654
HspAI	GCGC	5 cut(s)	198, 460, 505, 545, 619
KasI	GGCGCC	2 cut(s)	544, 618
KroI	GCCGGC	1 cut(s)	541
KroNI	GCCGGC	1 cut(s)	543
Ksp22I	TGATCA	1 cut(s)	957
KspI	CCGCGG	2 cut(s)	794, 847
LguI	GCTCTTC	3 cut(s)	756, 1001, 1625
LmnI	GCTCC	8 cut(s)	71, 94, 102, 445, 517, 613, 2542, 2704
Lsp1109I	GCAGC	8 cut(s)	337, 409, 435, 636, 918, 1485, 1843, 1846
MaeI	CTAG	4 cut(s)	1557, 1863, 1887, 2156
MaeII	ACGT	6 cut(s)	150, 654, 888, 1328, 2286, 2745
MaeIII	GTNAC	3 cut(s)	1273, 2225, 2522
MbiI	CCGCTC	1 cut(s)	616
MflI	RGATCY	4 cut(s)	60, 358, 786, 804
MhlI	GDGCHC	7 cut(s)	99, 522, 668, 1637, 1989, 2053, 2547
MlsI	TGGCCA	1 cut(s)	2772
MluCI	AATT	9 cut(s)	310, 1025, 1120, 2017, 2133, 2160, 2174, 2610, 2785
MluNI	TGGCCA	1 cut(s)	2772
Mly113I	GGCGCC	2 cut(s)	545, 619
MlyI	GAGTC	7 cut(s)	491, 499, 584, 608, 1170, 1385, 1972
MmeI	TCCRAC	1 cut(s)	696
Mox20I	TGGCCA	1 cut(s)	2772
Mph1103I	ATGCAT	2 cut(s)	1546, 2277
MreI	CGCCGGCG	1 cut(s)	541
MroNI	GCCGGC	1 cut(s)	541
MscI	TGGCCA	1 cut(s)	2772
MseI	TTAA	5 cut(s)	27, 1401, 1773, 2421, 2501
Msp20I	TGGCCA	1 cut(s)	2772
MspA1I	CMGCKG	3 cut(s)	793, 846, 2084
MspCI	CTTAAG	1 cut(s)	1772
Mva1269I	GAATGC	3 cut(s)	1459, 1510, 1544
MvaI	CCWGG	7 cut(s)	1199, 1893, 1913, 2639, 2702, 2760, 2774
MvnI	CGCG	5 cut(s)	462, 505, 793, 846, 2405
NaeI	GCCGGC	1 cut(s)	543
NarI	GGCGCC	2 cut(s)	545, 619
NciI	CCSGG	5 cut(s)	170, 832, 1429, 1510, 1678
NcoI	CCATGG	1 cut(s)	34
NdeI	CATATG	1 cut(s)	51
NgoMIV	GCCGGC	1 cut(s)	541
NlaIV	GGNNCC	7 cut(s)	360, 546, 620, 753, 788, 1897, 2778
NmeAIII	GCCGAG	6 cut(s)	331, 505, 513, 598, 713, 1173
NmuCI	GTSAC	1 cut(s)	1273
NruI	TCGCGA	1 cut(s)	2405
NsiI	ATGCAT	2 cut(s)	1546, 2277
PaqCI	CACCTGC	1 cut(s)	2240
PceI	AGGCCT	1 cut(s)	1891
PciSI	GCTCTTC	3 cut(s)	756, 1001, 1625
PcsI	WCGNNNNNNNCGW	8 cut(s)	159, 186, 491, 509, 648, 861, 864, 894
PctI	GAATGC	3 cut(s)	1459, 1510, 1544
PdiI	GCCGGC	1 cut(s)	543
PfeI	GAWTC	5 cut(s)	203, 988, 1384, 1792, 1995
PflFI	GACNNNGTC	4 cut(s)	653, 656, 736, 1423
PflMI	CCANNNNNTGG	1 cut(s)	1882
PfoI	TCCNGGA	2 cut(s)	2637, 2700
PleI	GAGTC	7 cut(s)	490, 498, 583, 607, 1169, 1385, 1971
PluTI	GGCGCC	2 cut(s)	548, 622
PpsI	GAGTC	7 cut(s)	490, 498, 583, 607, 1169, 1385, 1971
PshAI	GACNNNNGTC	1 cut(s)	149
PsiI	TTATAA	2 cut(s)	2132, 2570
Psp124BI	GAGCTC	2 cut(s)	99, 522
Psp6I	CCWGG	7 cut(s)	1197, 1891, 1911, 2637, 2700, 2758, 2772
PspFI	CCCAGC	1 cut(s)	135
PspGI	CCWGG	7 cut(s)	1197, 1891, 1911, 2637, 2700, 2758, 2772
PspN4I	GGNNCC	7 cut(s)	360, 546, 620, 753, 788, 1897, 2778
PspPI	GGNCC	4 cut(s)	550, 751, 781, 828
PstI	CTGCAG	2 cut(s)	453, 1503
PstNI	CAGNNNCTG	2 cut(s)	388, 1795
PsuI	RGATCY	4 cut(s)	60, 358, 786, 804
PsyI	GACNNNGTC	4 cut(s)	653, 656, 736, 1423
PvuII	CAGCTG	1 cut(s)	2084
RruI	TCGCGA	1 cut(s)	2405
RsaI	GTAC	5 cut(s)	902, 2350, 2387, 2601, 2691
RsaNI	GTAC	5 cut(s)	901, 2349, 2386, 2600, 2690
SacI	GAGCTC	2 cut(s)	99, 522
SacII	CCGCGG	2 cut(s)	794, 847
SapI	GCTCTTC	3 cut(s)	756, 1001, 1625
SaqAI	TTAA	5 cut(s)	27, 1401, 1773, 2421, 2501
Sau96I	GGNCC	4 cut(s)	550, 751, 781, 828
SchI	GAGTC	7 cut(s)	491, 499, 584, 608, 1170, 1385, 1972
SduI	GDGCHC	7 cut(s)	99, 522, 668, 1637, 1989, 2053, 2547
SfcI	CTRYAG	2 cut(s)	449, 1499
SfoI	GGCGCC	2 cut(s)	546, 620
Sfr303I	CCGCGG	2 cut(s)	794, 847
SgrAI	CRCCGGYG	1 cut(s)	541
SgrBI	CCGCGG	2 cut(s)	794, 847
SinI	GGWCC	3 cut(s)	550, 751, 828
SmlI	CTYRAG	3 cut(s)	1532, 1772, 2344
SmoI	CTYRAG	3 cut(s)	1532, 1772, 2344
Sse9I	AATT	9 cut(s)	310, 1025, 1120, 2017, 2133, 2160, 2174, 2610, 2785
SseBI	AGGCCT	1 cut(s)	1891
SspDI	GGCGCC	2 cut(s)	544, 618
SspI	AATATT	1 cut(s)	1753
SspMI	CTAG	4 cut(s)	1557, 1863, 1887, 2156
SstI	GAGCTC	2 cut(s)	99, 522
StuI	AGGCCT	1 cut(s)	1891
StyI	CCWWGG	1 cut(s)	34
TaaI	ACNGT	3 cut(s)	24, 2353, 2604
TaiI	ACGT	6 cut(s)	153, 657, 891, 1331, 2289, 2748
TaqI	TCGA	9 cut(s)	153, 189, 267, 368, 512, 704, 1586, 2529, 2679
TasI	AATT	9 cut(s)	310, 1025, 1120, 2017, 2133, 2160, 2174, 2610, 2785
TatI	WGTACW	2 cut(s)	900, 2348
TfiI	GAWTC	5 cut(s)	203, 988, 1384, 1792, 1995
Tru1I	TTAA	5 cut(s)	27, 1401, 1773, 2421, 2501
Tru9I	TTAA	5 cut(s)	27, 1401, 1773, 2421, 2501
TscAI	CASTG	5 cut(s)	301, 944, 1810, 2035, 2552
TseFI	GTSAC	1 cut(s)	1273
TseI	GCWGC	8 cut(s)	325, 397, 448, 624, 931, 1498, 1856, 1859
Tsp45I	GTSAC	1 cut(s)	1273
TspDTI	ATGAA	9 cut(s)	1210, 1751, 1763, 1830, 1894, 1921, 2087, 2351, 2645
TspGWI	ACGGA	6 cut(s)	161, 353, 568, 657, 2059, 2325
TspRI	CASTG	5 cut(s)	301, 944, 1810, 2035, 2552
Tth111I	GACNNNGTC	4 cut(s)	653, 656, 736, 1423
Van91I	CCANNNNNTGG	1 cut(s)	1882
Vha464I	CTTAAG	1 cut(s)	1772
VneI	GTGCAC	1 cut(s)	664
VpaK11BI	GGWCC	3 cut(s)	550, 751, 828
XapI	RAATTY	3 cut(s)	310, 1120, 2174
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1258
XspI	CTAG	4 cut(s)	1557, 1863, 1887, 2156
ZraI	GACGTC	2 cut(s)	151, 655
Zsp2I	ATGCAT	2 cut(s)	1546, 2277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.