Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Reverse (-)

21018243 .. 21022219

Gene Structure 3977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1129900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGGATTTCTCCATCAAGAAATCGTTTAAATCCCATGGCTCTGCCAAGCACACGAGGAAGATCTCCGCCGGAGCAGACGACCCCAACCACGAGGAGCTCCCTATTCTTTTAGATCACGACCACCGCCAACACAGCCAGCCGATGACTGACCTCGACTTGTCCGACCGGCGGGAAGTCATTGTCAAGATCGACGACGGTGCTGATTCCTCCACCAGCGCAACCATGGACCCCGCCAAAAATGGCGGGAAGATTTGGCGGGAGTCCAGCATCGATTTCTGGAACGGGGACAGCAACGACGACGGCAATGCCGAGAGTTTCGATTTCGTGCAGCGCGGGAAGACGGCCACGGTGCCGGGGGAGGATCCGCCGTCGAAGCTGATTGGGCAGTTCCTGCACAAGCAGAGAGCCTCCGGCGACATGACGCTTGACATGGATTTGGATATGGAGGAGCTGCGGCAGAACGAGCGCGAGTTGCCTCCGCTTGCCGAGTTGCCGAGTCCGATGGACTCGCGAAAAGTTTCGTTCCAATCGCCGGCGTCGGACCTCATCGATACACCGCAAACCGAGTCGGTTCGGAGGCGATACAGGGAGTCCCCAAACGAGGAGCGGCGCCGCAGCGGGAGGTTGAGCAATGGACAGGACGACGTCGTGCGCTGTACTTCCAACGCCTCATTCCGCCGAGAGCCTTCGTTCGCGAACAAGTCCGATTTGCTGAGAATAAGGACCAAGTCCAGACTAATCGACCCGCCCGAGGAGCCGGACTTCAAATCGGGCAGAATCCCGCGGTCAGGCCAGATCCCGAGATCCGGCCTGATGAGGTCCGGGTTGCTGAGCCGCGGCGGCGACGACGAGGACGACGACCCGTTTCTGGAGGAGGACGTGCCGCACAAGTACAAGAGGGCGAAGTTCAATGCATGGACGCTGCTTCAATGGGTAAGCTTGATTTTGATCATTGGGGCTCTGGTTTGCACGCTGACGATTCCAGTTCTGAGGCAGAAGAGCTTGTGGAAATTGATATTGTGGAAGTGGGAGGTCTTGATTTTGGTTCTGATTTGCGGGAGATTGGTTTCGGGTTGGGGGATTAGGATCATAGTGTTTTTTGTGGAGAGGAATTTCTATCTGCGGAAGAGGATTTTGTATTTCGTATATGGAGTCCGAAAGGCGGTGCAGAACTGCCTCTGGCTCGGCCTGGTTCTAATCGCATGGCATTTCATGTTTGATAAGAAGGTGGAGAGGGAGACCAAGAGCGAGGTGCCTGCCTATGTGACGAAAATCCTGCTTTGTCTCTTGATTGGAGTGTTGTTGTGGCTGGTGAAGACGTTGATTGTGAAGGTGTTGGCTTCGTCGTTCCATGTGAACTCGTATTTTGATCGGATTCAGGACGCACTTTTTAATCAGTATGTGATTGAGACGCTGTCCGGGCGGCCTTTGATTGAGATACAGAATGCGGAGGAGGAGGAGGAGAAGTTGGCGGATGAGGTCAGGAAGCTGCAGAGTGCCGGGGCTACTATGCCTCCTGCCCTCAAGGCAAATGCATTCCCGTCCCCGAGGATTGGGAGGGATTCGATGAGGAGTGAGAGGGTGATTGCGAGTAGTGGGCTTATTGGGAAGAGCACCAAGTATTCTAGGCCCCTTTCCAAGAGGACAGAAGAGACCGGGATCACGATTGATCACTTGCATAAGCTCAATCCCAAGAATGTGTCTGCTTGGAATATGAAGAGGCTGATGAATATAGTTAGAAAGGGGCATCTTACGACACTTGATGAGCAGATTCTGGATGCCACTGGTGAGGATGAGGCCGATACCCAGATCAGGAGTGAAGTTGAAGCAAAGGCTGCTGCTAAAAAGATATTTCAAAATGTAGCTAGGCGTGGTTCCAAGTACATCTACCAGGAGGATTTGATGTGTTTCATGGAAGAAGACGAGGCTGTGAAGACCATGAGTCTCTTTGAAGGGGCATCTGAGCACAAAAGAATCAGCAAATCTGCCTTGAAAAATTGGGTGGTCAATGCCTTCAGAGAACGGAGAGCACTTGCTTTGACGCTGAATGACACCAAAACAGCTGTGAATACACTTCATCGCATGGTGAACATAATAGTTGCCATTGTTATAGTAGTAATATGGCTAATTGTGCTGGGAATTGCCACCACCAAGTTTCTCTTGTTTGTGAGTTCTCAGCTTGTGGTTTTGGCATTTGTCTTTGGCAACACCTGTAAGACGATATTTGAAGCAGTCATATTTTTATTCGTGAGGCACCCCTTTGACGTGGGAGATCGTTGCGAAATAGATGGAGTTCAGATGGTAGTTGAAGAAATGAACATCTTGTCTACAGTTTTCCTAAGGTATGACAACACGAAAATCGTGTACCCAAACAGCATCCTCGCGACTCTGCCCATATTTAACTTCTATCGTAGTCCTGACACGGGTGATGCTATTGAGTTCTGCATTCACATATCAACTCCACCAGATAGGATTGTTTTAATGAGGCAAAGAATAATCAGTTACATCGAGAACAAGAAGGAGCACTGGTATCCAGCACCAATGATAATAATGAAGGATGTAGAACAACTAAACAGGGTAAGGTTTGCAATTTGGCCTCAACATAGAATGAACTTCCAGGACATAGGAGAAAGGTGGGTAAGGAGAGGCTATTTGGTCGAAGAGATGGTACGTATATTCCAGGAGCTTGACATCCAGTACCGCCTTCTGCCTATTGACATAAATGTCCGCGGCATGCCTGGCATGACTGGCCAGGCTCCCTCTAATTACGCGGCAACTAAAGCCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

930

Amino Acids

106.3

Weight (kDa)

8.57

Isoelectric Point (pI)

49.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	746 - 802	1e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2723
AatII	GACGTC	1 cut(s)	648
AccB1I	GGYRCC	4 cut(s)	349, 609, 1252, 2253
AccBSI	CCGCTC	1 cut(s)	607
AccI	GTMKAC	1 cut(s)	2327
AccII	CGCG	9 cut(s)	333, 468, 511, 695, 784, 837, 2384, 2730, 2771
AclWI	GGATC	6 cut(s)	356, 369, 790, 798, 1094, 1667
AcoI	YGGCCR	2 cut(s)	342, 2749
AcsI	RAATTY	1 cut(s)	1111
AcuI	CTGAAG	1 cut(s)	1999
AcyI	GRCGYC	3 cut(s)	536, 610, 645
AfaI	GTAC	6 cut(s)	658, 893, 1883, 2366, 2670, 2699
AgsI	TTSAA	9 cut(s)	766, 910, 929, 1826, 1856, 1952, 1993, 2228, 2309
AhdI	GACNNNNNGTC	1 cut(s)	1941
AjiI	CACGTC	2 cut(s)	880, 2266
AjnI	CCWGG	6 cut(s)	1188, 1890, 2616, 2679, 2737, 2751
AjuI	GAANNNNNNNTTGG	2 cut(s)	656, 688
Alw21I	GWGCWC	5 cut(s)	99, 1616, 1968, 2032, 2526
Alw26I	GTCTC	5 cut(s)	1232, 1289, 1403, 1646, 1949
AlwI	GGATC	6 cut(s)	356, 369, 790, 798, 1094, 1667
AlwNI	CAGNNNCTG	2 cut(s)	391, 1774
Ama87I	CYCGRG	3 cut(s)	749, 799, 1546
AoxI	GGCC	9 cut(s)	342, 790, 808, 1186, 1424, 1628, 1797, 2594, 2749
ApeKI	GCWGC	7 cut(s)	328, 451, 615, 922, 1489, 1835, 1838
ApoI	RAATTY	1 cut(s)	1111
Asp700I	GAANNNNTTC	1 cut(s)	517
AspLEI	GCGC	5 cut(s)	218, 333, 468, 612, 654
AspS9I	GGNCC	5 cut(s)	226, 541, 723, 819, 1629
AsuC2I	CCSGG	5 cut(s)	354, 823, 1420, 1501, 1657
AsuHPI	GGTGA	5 cut(s)	1324, 1594, 1799, 2098, 2438
AvaI	CYCGRG	3 cut(s)	749, 799, 1546
AvaII	GGWCC	4 cut(s)	226, 541, 723, 819
AxyI	CCTNAGG	1 cut(s)	2339
BalI	TGGCCA	1 cut(s)	2751
BamHI	GGATCC	1 cut(s)	361
BanI	GGYRCC	4 cut(s)	349, 609, 1252, 2253
BanII	GRGCYC	2 cut(s)	99, 961
BarI	GAAGNNNNNNTAC	2 cut(s)	2652, 2684
BauI	CACGAG	2 cut(s)	52, 89
BbsI	GAAGAC	4 cut(s)	344, 1322, 1926, 1940
Bbv12I	GWGCWC	5 cut(s)	99, 1616, 1968, 2032, 2526
BbvI	GCAGC	7 cut(s)	340, 438, 627, 909, 1476, 1822, 1825
BccI	CCATC	5 cut(s)	20, 496, 2282, 2293, 2659
BceAI	ACGGC	3 cut(s)	316, 352, 357
BcgI	CGANNNNNNTGC	8 cut(s)	179, 213, 287, 321, 1238, 1258, 1272, 1292
BciT130I	CCWGG	6 cut(s)	1190, 1892, 2618, 2681, 2739, 2753
BciVI	GTATCC	1 cut(s)	2541
BclI	TGATCA	2 cut(s)	948, 1669
BcnI	CCSGG	5 cut(s)	354, 823, 1420, 1501, 1657
BcoDI	GTCTC	5 cut(s)	1232, 1289, 1403, 1646, 1949
BfaI	CTAG	2 cut(s)	1626, 1866
BfmI	CTRYAG	2 cut(s)	1490, 2328
BfoI	RGCGCY	1 cut(s)	613
BfuI	GTATCC	1 cut(s)	2541
BglI	GCCNNNNNGGC	2 cut(s)	840, 1526
BglII	AGATCT	1 cut(s)	60
BlpI	GCTNAGC	1 cut(s)	830
Bme18I	GGWCC	4 cut(s)	226, 541, 723, 819
BmeRI	GACNNNNNGTC	1 cut(s)	1941
BmeT110I	CYCGRG	3 cut(s)	749, 799, 1546
BmgBI	CACGTC	2 cut(s)	880, 2266
BmgT120I	GGNCC	5 cut(s)	226, 541, 723, 819, 1629
BmsI	GCATC	6 cut(s)	276, 1756, 1768, 1967, 2385, 2419
BpiI	GAAGAC	4 cut(s)	344, 1322, 1926, 1940
BpmI	CTGGAG	1 cut(s)	890
Bpu1102I	GCTNAGC	1 cut(s)	830
BpuEI	CTTGAG	1 cut(s)	1508
BpuMI	CCSGG	5 cut(s)	354, 823, 1420, 1501, 1657
Bsa29I	ATCGAT	2 cut(s)	270, 549
BsaAI	YACGTR	1 cut(s)	2672
BsaBI	GATNNNNATC	2 cut(s)	947, 1085
BsaHI	GRCGYC	3 cut(s)	536, 610, 645
BsaI	GGTCTC	2 cut(s)	1232, 1646
BsaXI	ACNNNNNCTCC	6 cut(s)	1451, 1481, 1498, 1528, 2513, 2543
Bse118I	RCCGGY	2 cut(s)	165, 532
Bse1I	ACTGG	5 cut(s)	983, 1789, 2531, 2695, 2752
Bse21I	CCTNAGG	1 cut(s)	2339
Bse3DI	GCAATG	2 cut(s)	310, 637
Bse8I	GATNNNNATC	2 cut(s)	947, 1085
BseBI	CCWGG	6 cut(s)	1190, 1892, 2618, 2681, 2739, 2753
BseCI	ATCGAT	2 cut(s)	270, 549
BseGI	GGATG	6 cut(s)	1480, 1783, 1798, 2376, 2563, 2691
BseJI	GATNNNNATC	2 cut(s)	947, 1085
BseMI	GCAATG	2 cut(s)	310, 637
BseMII	CTCAG	5 cut(s)	704, 821, 980, 1953, 2189
BseNI	ACTGG	5 cut(s)	983, 1789, 2531, 2695, 2752
BseXI	GCAGC	7 cut(s)	340, 438, 627, 909, 1476, 1822, 1825
BseYI	CCCAGC	1 cut(s)	2134
BsgI	GTGCAG	3 cut(s)	347, 377, 1187
Bsh1236I	CGCG	9 cut(s)	333, 468, 511, 695, 784, 837, 2384, 2730, 2771
Bsh1285I	CGRYCG	1 cut(s)	166
BshFI	GGCC	9 cut(s)	344, 792, 810, 1188, 1426, 1630, 1799, 2596, 2751
BshNI	GGYRCC	4 cut(s)	349, 609, 1252, 2253
BshVI	ATCGAT	2 cut(s)	270, 549
BsiEI	CGRYCG	1 cut(s)	166
BsiHKAI	GWGCWC	5 cut(s)	99, 1616, 1968, 2032, 2526
BsiHKCI	CYCGRG	3 cut(s)	749, 799, 1546
BslFI	GGGAC	3 cut(s)	299, 577, 1528
BsmAI	GTCTC	5 cut(s)	1232, 1289, 1403, 1646, 1949
BsmBI	CGTCTC	1 cut(s)	1403
BsmFI	GGGAC	3 cut(s)	299, 577, 1528
BsmI	GAATGC	3 cut(s)	1450, 1535, 2445
BsnI	GGCC	9 cut(s)	344, 792, 810, 1188, 1426, 1630, 1799, 2596, 2751
Bso31I	GGTCTC	2 cut(s)	1232, 1646
BsoBI	CYCGRG	3 cut(s)	749, 799, 1546
Bsp1286I	GDGCHC	6 cut(s)	99, 961, 1616, 1968, 2032, 2526
Bsp1720I	GCTNAGC	1 cut(s)	830
Bsp19I	CCATGG	2 cut(s)	34, 222
Bsp68I	TCGCGA	3 cut(s)	511, 695, 2384
BspANI	GGCC	9 cut(s)	344, 792, 810, 1188, 1426, 1630, 1799, 2596, 2751
BspCNI	CTCAG	5 cut(s)	705, 822, 981, 1954, 2188
BspDI	ATCGAT	2 cut(s)	270, 549
BspFNI	CGCG	9 cut(s)	333, 468, 511, 695, 784, 837, 2384, 2730, 2771
BspMAI	CTGCAG	1 cut(s)	1494
BspPI	GGATC	6 cut(s)	356, 369, 790, 798, 1094, 1667
BspQI	GCTCTTC	2 cut(s)	992, 1604
BspT107I	GGYRCC	4 cut(s)	349, 609, 1252, 2253
BspTNI	GGTCTC	2 cut(s)	1232, 1646
BsrBI	CCGCTC	1 cut(s)	607
BsrDI	GCAATG	2 cut(s)	310, 637
BsrFI	RCCGGY	2 cut(s)	165, 532
BsrI	ACTGG	5 cut(s)	983, 1789, 2531, 2695, 2752
BssAI	RCCGGY	2 cut(s)	165, 532
BssNI	GRCGYC	3 cut(s)	536, 610, 645
BssSI	CACGAG	2 cut(s)	52, 89
BssT1I	CCWWGG	2 cut(s)	34, 222
Bst2BI	CACGAG	2 cut(s)	52, 89
Bst2UI	CCWGG	6 cut(s)	1190, 1892, 2618, 2681, 2739, 2753
Bst4CI	ACNGT	3 cut(s)	197, 349, 2332
Bst6I	CTCTTC	6 cut(s)	992, 1121, 1604, 1644, 1712, 2655
BstACI	GRCGYC	3 cut(s)	536, 610, 645
BstAPI	GCANNNNNTGC	2 cut(s)	391, 1835
BstBAI	YACGTR	1 cut(s)	2672
BstC8I	GCNNGC	6 cut(s)	137, 483, 534, 971, 1257, 2735
BstDEI	CTNAG	6 cut(s)	713, 830, 989, 1962, 2175, 2339
BstDSI	CCRYGG	6 cut(s)	34, 222, 345, 782, 835, 2728
BstF5I	GGATG	6 cut(s)	1480, 1783, 1798, 2376, 2563, 2691
BstFNI	CGCG	9 cut(s)	333, 468, 511, 695, 784, 837, 2384, 2730, 2771
BstH2I	RGCGCY	1 cut(s)	613
BstHHI	GCGC	5 cut(s)	218, 333, 468, 612, 654
BstMAI	GTCTC	5 cut(s)	1232, 1289, 1403, 1646, 1949
BstMCI	CGRYCG	1 cut(s)	166
BstNI	CCWGG	6 cut(s)	1190, 1892, 2618, 2681, 2739, 2753
BstNSI	RCATGY	1 cut(s)	2737
BstSFI	CTRYAG	2 cut(s)	1490, 2328
BstSNI	TACGTA	1 cut(s)	2672
BstUI	CGCG	9 cut(s)	333, 468, 511, 695, 784, 837, 2384, 2730, 2771
BstV1I	GCAGC	7 cut(s)	340, 438, 627, 909, 1476, 1822, 1825
BstV2I	GAAGAC	4 cut(s)	344, 1322, 1926, 1940
BstX2I	RGATCY	4 cut(s)	60, 361, 795, 803
BstYI	RGATCY	4 cut(s)	60, 361, 795, 803
Bsu15I	ATCGAT	2 cut(s)	270, 549
Bsu36I	CCTNAGG	1 cut(s)	2339
BsuI	GTATCC	1 cut(s)	2541
BsuRI	GGCC	9 cut(s)	344, 792, 810, 1188, 1426, 1630, 1799, 2596, 2751
BsuTUI	ATCGAT	2 cut(s)	270, 549
BtgI	CCRYGG	6 cut(s)	34, 222, 345, 782, 835, 2728
BtgZI	GCGATG	1 cut(s)	2063
BtrI	CACGTC	2 cut(s)	880, 2266
BtsCI	GGATG	6 cut(s)	1480, 1783, 1798, 2376, 2563, 2691
BtsIMutI	CAGTG	2 cut(s)	1782, 2524
BtuMI	TCGCGA	3 cut(s)	511, 695, 2384
Cac8I	GCNNGC	6 cut(s)	137, 483, 534, 971, 1257, 2735
CaiI	CAGNNNCTG	2 cut(s)	391, 1774
CfoI	GCGC	5 cut(s)	218, 333, 468, 612, 654
Cfr10I	RCCGGY	2 cut(s)	165, 532
Cfr13I	GGNCC	5 cut(s)	226, 541, 723, 819, 1629
Cfr42I	CCGCGG	3 cut(s)	785, 838, 2731
ClaI	ATCGAT	2 cut(s)	270, 549
CseI	GACGC	6 cut(s)	430, 525, 928, 1391, 1420, 2050
Csp6I	GTAC	6 cut(s)	657, 892, 1882, 2365, 2669, 2698
CviQI	GTAC	6 cut(s)	657, 892, 1882, 2365, 2669, 2698
DdeI	CTNAG	6 cut(s)	713, 830, 989, 1962, 2175, 2339
DinI	GGCGCC	1 cut(s)	611
DraI	TTTAAA	1 cut(s)	28
DrdI	GACNNNNNNGTC	1 cut(s)	2723
DriI	GACNNNNNGTC	1 cut(s)	1941
DseDI	GACNNNNNNGTC	1 cut(s)	2723
EaeI	YGGCCR	2 cut(s)	342, 2749
Eam1104I	CTCTTC	6 cut(s)	992, 1121, 1604, 1644, 1712, 2655
Eam1105I	GACNNNNNGTC	1 cut(s)	1941
EarI	CTCTTC	6 cut(s)	992, 1121, 1604, 1644, 1712, 2655
EciI	GGCGGA	4 cut(s)	55, 354, 665, 1487
Ecl136II	GAGCTC	1 cut(s)	97
Eco105I	TACGTA	1 cut(s)	2672
Eco130I	CCWWGG	2 cut(s)	34, 222
Eco24I	GRGCYC	2 cut(s)	99, 961
Eco31I	GGTCTC	2 cut(s)	1232, 1646
Eco47I	GGWCC	4 cut(s)	226, 541, 723, 819
Eco53kI	GAGCTC	1 cut(s)	97
Eco57I	CTGAAG	1 cut(s)	1999
Eco81I	CCTNAGG	1 cut(s)	2339
Eco88I	CYCGRG	3 cut(s)	749, 799, 1546
EcoICRI	GAGCTC	1 cut(s)	97
EcoO109I	RGGNCCY	1 cut(s)	1629
EcoRII	CCWGG	6 cut(s)	1188, 1890, 2616, 2679, 2737, 2751
EcoT14I	CCWWGG	2 cut(s)	34, 222
EcoT22I	ATGCAT	2 cut(s)	916, 1537
EcoT38I	GRGCYC	2 cut(s)	99, 961
EgeI	GGCGCC	1 cut(s)	611
EheI	GGCGCC	1 cut(s)	611
ErhI	CCWWGG	2 cut(s)	34, 222
Esp3I	CGTCTC	1 cut(s)	1403
FalI	AAGNNNNNCTT	2 cut(s)	2144, 2176
FaqI	GGGAC	3 cut(s)	299, 577, 1528
FauI	CCCGC	9 cut(s)	162, 236, 238, 249, 326, 611, 753, 789, 1049
FbaI	TGATCA	2 cut(s)	948, 1669
FblI	GTMKAC	1 cut(s)	2327
FokI	GGATG	6 cut(s)	1487, 1790, 1805, 2363, 2570, 2678
FriOI	GRGCYC	2 cut(s)	99, 961
FspBI	CTAG	2 cut(s)	1626, 1866
GlaI	GCGC	5 cut(s)	217, 332, 467, 611, 653
GsaI	CCCAGC	1 cut(s)	2138
GsuI	CTGGAG	1 cut(s)	890
HaeII	RGCGCY	1 cut(s)	613
HaeIII	GGCC	9 cut(s)	344, 792, 810, 1188, 1426, 1630, 1799, 2596, 2751
HgaI	GACGC	6 cut(s)	430, 525, 928, 1391, 1420, 2050
HhaI	GCGC	5 cut(s)	218, 333, 468, 612, 654
Hin1I	GRCGYC	3 cut(s)	536, 610, 645
Hin6I	GCGC	5 cut(s)	216, 331, 466, 610, 652
HinP1I	GCGC	5 cut(s)	216, 331, 466, 610, 652
HindIII	AAGCTT	1 cut(s)	937
HphI	GGTGA	5 cut(s)	1324, 1594, 1799, 2098, 2438
Hpy166II	GTNNAC	4 cut(s)	1357, 2089, 2328, 2365
Hpy8I	GTNNAC	4 cut(s)	1357, 2089, 2328, 2365
HpyAV	CCTTC	8 cut(s)	696, 1219, 1324, 1946, 2023, 2512, 2548, 2714
HpyCH4III	ACNGT	3 cut(s)	197, 349, 2332
HpyCH4IV	ACGT	5 cut(s)	645, 879, 1319, 2265, 2671
HpyF3I	CTNAG	6 cut(s)	713, 830, 989, 1962, 2175, 2339
HpySE526I	ACGT	5 cut(s)	645, 879, 1319, 2265, 2671
Hsp92I	GRCGYC	3 cut(s)	536, 610, 645
HspAI	GCGC	5 cut(s)	216, 331, 466, 610, 652
KasI	GGCGCC	1 cut(s)	609
KroI	GCCGGC	1 cut(s)	532
KroNI	GCCGGC	1 cut(s)	534
Ksp22I	TGATCA	2 cut(s)	948, 1669
KspI	CCGCGG	3 cut(s)	785, 838, 2731
LguI	GCTCTTC	2 cut(s)	992, 1604
LmnI	GCTCC	9 cut(s)	71, 94, 102, 448, 604, 754, 2521, 2683, 2761
Lsp1109I	GCAGC	7 cut(s)	340, 438, 627, 909, 1476, 1822, 1825
LweI	GCATC	6 cut(s)	276, 1756, 1768, 1967, 2385, 2419
MaeI	CTAG	2 cut(s)	1626, 1866
MaeII	ACGT	5 cut(s)	645, 879, 1319, 2265, 2671
MaeIII	GTNAC	2 cut(s)	1264, 2501
MbiI	CCGCTC	1 cut(s)	607
MflI	RGATCY	4 cut(s)	60, 361, 795, 803
MhlI	GDGCHC	6 cut(s)	99, 961, 1616, 1968, 2032, 2526
MlsI	TGGCCA	1 cut(s)	2751
MluCI	AATT	7 cut(s)	1010, 1111, 1996, 2127, 2139, 2589, 2764
MluNI	TGGCCA	1 cut(s)	2751
Mly113I	GGCGCC	1 cut(s)	610
MlyI	GAGTC	8 cut(s)	269, 500, 505, 575, 599, 1161, 1951, 2380
MmeI	TCCRAC	3 cut(s)	186, 519, 687
Mox20I	TGGCCA	1 cut(s)	2751
Mph1103I	ATGCAT	2 cut(s)	916, 1537
MreI	CGCCGGCG	1 cut(s)	532
MroNI	GCCGGC	1 cut(s)	532
MroXI	GAANNNNTTC	1 cut(s)	517
MscI	TGGCCA	1 cut(s)	2751
MseI	TTAA	4 cut(s)	27, 1392, 2400, 2480
Msp20I	TGGCCA	1 cut(s)	2751
MspA1I	CMGCKG	5 cut(s)	618, 784, 837, 2063, 2730
Mva1269I	GAATGC	3 cut(s)	1450, 1535, 2445
MvaI	CCWGG	6 cut(s)	1190, 1892, 2618, 2681, 2739, 2753
MvnI	CGCG	9 cut(s)	333, 468, 511, 695, 784, 837, 2384, 2730, 2771
NaeI	GCCGGC	1 cut(s)	534
NarI	GGCGCC	1 cut(s)	610
NciI	CCSGG	5 cut(s)	354, 823, 1420, 1501, 1657
NcoI	CCATGG	2 cut(s)	34, 222
NgoMIV	GCCGGC	1 cut(s)	532
NmeAIII	GCCGAG	5 cut(s)	334, 511, 519, 704, 1164
NmuCI	GTSAC	1 cut(s)	1264
NruI	TCGCGA	3 cut(s)	511, 695, 2384
NsiI	ATGCAT	2 cut(s)	916, 1537
NspI	RCATGY	1 cut(s)	2737
PaeI	GCATGC	1 cut(s)	2737
PciSI	GCTCTTC	2 cut(s)	992, 1604
PcsI	WCGNNNNNNNCGW	5 cut(s)	159, 306, 747, 852, 855
PctI	GAATGC	3 cut(s)	1450, 1535, 2445
PdiI	GCCGGC	1 cut(s)	534
PdmI	GAANNNNTTC	1 cut(s)	517
PfeI	GAWTC	7 cut(s)	203, 777, 979, 1375, 1562, 1771, 1974
PflFI	GACNNNGTC	3 cut(s)	644, 727, 1414
PfoI	TCCNGGA	2 cut(s)	2616, 2679
PleI	GAGTC	8 cut(s)	268, 500, 504, 574, 598, 1160, 1950, 2380
PluTI	GGCGCC	1 cut(s)	613
PpsI	GAGTC	8 cut(s)	268, 500, 504, 574, 598, 1160, 1950, 2380
Ppu21I	YACGTR	1 cut(s)	2672
Psp124BI	GAGCTC	1 cut(s)	99
Psp6I	CCWGG	6 cut(s)	1188, 1890, 2616, 2679, 2737, 2751
PspFI	CCCAGC	1 cut(s)	2134
PspGI	CCWGG	6 cut(s)	1188, 1890, 2616, 2679, 2737, 2751
PspPI	GGNCC	5 cut(s)	226, 541, 723, 819, 1629
PstI	CTGCAG	1 cut(s)	1494
PstNI	CAGNNNCTG	2 cut(s)	391, 1774
PsuI	RGATCY	4 cut(s)	60, 361, 795, 803
PsyI	GACNNNGTC	3 cut(s)	644, 727, 1414
PvuII	CAGCTG	1 cut(s)	2063
RruI	TCGCGA	3 cut(s)	511, 695, 2384
RsaI	GTAC	6 cut(s)	658, 893, 1883, 2366, 2670, 2699
RsaNI	GTAC	6 cut(s)	657, 892, 1882, 2365, 2669, 2698
SacI	GAGCTC	1 cut(s)	99
SacII	CCGCGG	3 cut(s)	785, 838, 2731
SapI	GCTCTTC	2 cut(s)	992, 1604
SaqAI	TTAA	4 cut(s)	27, 1392, 2400, 2480
Sau96I	GGNCC	5 cut(s)	226, 541, 723, 819, 1629
SchI	GAGTC	8 cut(s)	269, 500, 505, 575, 599, 1161, 1951, 2380
SduI	GDGCHC	6 cut(s)	99, 961, 1616, 1968, 2032, 2526
SfaNI	GCATC	6 cut(s)	276, 1756, 1768, 1967, 2385, 2419
SfcI	CTRYAG	2 cut(s)	1490, 2328
SfoI	GGCGCC	1 cut(s)	611
Sfr303I	CCGCGG	3 cut(s)	785, 838, 2731
SgrAI	CRCCGGYG	1 cut(s)	532
SgrBI	CCGCGG	3 cut(s)	785, 838, 2731
SinI	GGWCC	4 cut(s)	226, 541, 723, 819
SmlI	CTYRAG	1 cut(s)	1523
SmoI	CTYRAG	1 cut(s)	1523
SnaBI	TACGTA	1 cut(s)	2672
SphI	GCATGC	1 cut(s)	2737
Sse9I	AATT	7 cut(s)	1010, 1111, 1996, 2127, 2139, 2589, 2764
SspDI	GGCGCC	1 cut(s)	609
SspMI	CTAG	2 cut(s)	1626, 1866
SstI	GAGCTC	1 cut(s)	99
StyI	CCWWGG	2 cut(s)	34, 222
TaaI	ACNGT	3 cut(s)	197, 349, 2332
TaiI	ACGT	5 cut(s)	648, 882, 1322, 2268, 2674
TasI	AATT	7 cut(s)	1010, 1111, 1996, 2127, 2139, 2589, 2764
TatI	WGTACW	3 cut(s)	656, 891, 1881
TfiI	GAWTC	7 cut(s)	203, 777, 979, 1375, 1562, 1771, 1974
Tru1I	TTAA	4 cut(s)	27, 1392, 2400, 2480
Tru9I	TTAA	4 cut(s)	27, 1392, 2400, 2480
TscAI	CASTG	2 cut(s)	1789, 2531
TseFI	GTSAC	1 cut(s)	1264
TseI	GCWGC	7 cut(s)	328, 451, 615, 922, 1489, 1835, 1838
Tsp45I	GTSAC	1 cut(s)	1264
TspDTI	ATGAA	8 cut(s)	1201, 1730, 1742, 1900, 2066, 2330, 2567, 2624
TspGWI	ACGGA	1 cut(s)	2038
TspRI	CASTG	2 cut(s)	1789, 2531
Tth111I	GACNNNGTC	3 cut(s)	644, 727, 1414
VpaK11BI	GGWCC	4 cut(s)	226, 541, 723, 819
XapI	RAATTY	1 cut(s)	1111
XceI	RCATGY	1 cut(s)	2737
XcmI	CCANNNNNNNNNTGG	1 cut(s)	220
XmiI	GTMKAC	1 cut(s)	2327
XmnI	GAANNNNTTC	1 cut(s)	517
XspI	CTAG	2 cut(s)	1626, 1866
ZraI	GACGTC	1 cut(s)	646
Zsp2I	ATGCAT	2 cut(s)	916, 1537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.