Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Forward (+)

26920703 .. 26924726

Gene Structure 4024 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000013165

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2772 bp

ATGGATTTCTCACTCAAGAAATCCTTCAAATCTCTCGGCTCCGGTAAACACATCTCTGCTGAAGCAGACCACAGCAGCCACGAGAAGCTCCCCATTCTAATGGATCACCACAACGAACAGCACGCAATGAGCGCCGTCGATTCCTCCGACCGCCGCGAGGTCATCGTCAAGATCGACGAGGGCGGCGCTTCCTCCACCACCACTACTAGAGAAGCCGACCCCGCCAAACGCGGCGCCGGGATTTGGAGGGAGTCCAGTATCGATTTCTGGAAGGACGACGGGAGCAATGCCGGCGCCGGCGGGGAATTTGAGTTTGCTCAGAAGAAGAAGACGGCGGCGAAGACGGAGAAGGAGGAGGACGATCCACCGTCGAAGCTGATAGGGAGGTTCCTGCACAAGCAGAGAGCCTCCGGCGACTCGTCATTGGACATGGATTTGGAGATGGACGAGCTACGGCCGCAGGATTTGCCGCCGCTGGCGGAGTCCCCGAGGAACTCTCGTATGTCGAGGGAGATCAAGGTGTCGTTCCAGTCGTTCCAGTCGCCGGTGTCGGACGTCGCCGAGACCGAGTCGATTCGGCGGCGGCGCAAGGACTCCGAGGACGAGGAACGACGTCGTAGCAGTCGACTGAGTGACGGCGGCGCCGACGTCGTTAGGTGCTCGTCGAACGCGTCGTTTAAGAGCAGGTCCGATTTGTTGAGGCTGAAGACGAGGTCCAGATTGATGGACCCGCCCGAGGGGTGGGACGAGGGTGCGGGTCGGGTCCCGAAATCGGGTATGATGAGGTCTGGGATGTTGGGCCGGGCCGGAGGTGACGATGAGGAGGACGACCCGTTTCTGGAGGAGGATTTGCCTGATGAGTACAAGAGGGCTAAGTTCAATGCACTGACTCTGCTCCAGTGGCTGAGTTTGGTTTTGATTATTGGGGCATTGGCTTGCACTCTTTCCATAAGAGTTTTGAGGGAGAAGAATCTGTGGAAATTGAAGCTGTGGAAATGGGAGGTGCTGATTTTGGTTTCGATTTGTGGGAGGCTGGTTTCGGGTTGGATGATAAAAATTATGGTGTTCTTTGTGGAGAGGAATTTCATTTTGAGGAAGAGGGTGTTGTATTTTGTTTATGGAATCAGGAAACCTGTTCAGAACTGTATTTGGCTTGGCCTTGTTTTGACGGGTTGGCATTTCTTGCTGATCAAGAAGGTTGAGAAGGAAACTAATGGTAGAATCCTTGAGTATGTGACTAAAGTGTTGCTTTGTCTCTTGATTGGGGGTTTGATTTGGTTGGTGAAGACCCTGGTTGTAAAAGTGCTTGCTTCGTCTTTCCATGTTAGATCATATTTTGATCGGATTCAGGATGCGCTTTTTAATCAGTATGTGATCCAAACACTGTCGGGGCGGCCTTGGATTGAGATAAAAAATGCAGAGGATGAAGAGGAGAGACTTGCTGATGAGGTTAGGAAGCTACAGAATGCTGGGGCTAATATGCCTGCTGACCTCAGAGCAAGTGCATTCCCTTCTGGGACAACTGGGAAGGCGGTTGGGAATGGCTCTGGTAGAAGTGGGCGGTTGATTGCTGCAAGTGGAGTGCTTGTGGGGAAGAGTGGCATGTTTTCAAAGCCACTTTCCAAGAAGTCAGGGGAGGTCGGGATCACAATTGATCACTTGCATAGGCTCAATCCAAAGAATGTGTCTGCTTGGAATATGAAGAGGCTAATGAATATAGTAAAATATGGGCATCTTACCACACTGGATGAGCAGATTCTGGATTCCACCAAGGATGAGGGTGATACTACGATCAAAAGTGAAGTTGAGGCAAAGGCTGCCGCGAAAAAGATATTTCGCAATGTGGCTAGGCCTGGCTCCAAGCACATCTACCTGGAGGATTTGTTGCGTTTCATGCAAGAAGACGAGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCTGAGAGCAAGAGAATCAGCAAAACGTCCTTGAAAAATTGGGTGGTCAATTGCTTTAGAGAACGGAGAGCACTTGCTTTGACTCTAACTGACACCAAAACAGCGGTGAACAGACTTCGACGTGTGGTGGACGTATTAGTTAGCATTATTATTATAATTATATGGCTTCTTGTGCTGGATATTGTCACCAGAGCCAATATTGTTTATGCAACTTCTCAGCTTGTGATTGTGGCATTTGTTTTTGGTAACTCCTGCAAGACAGTCTTTGAAGCAGTCATATTTCTATTCGTGATGCACCCCTTTGACGTGGGAGATCGATGTGAAATTGAAGGAGTTCAGATGGTTGTAGAGGAGATGAATATCTTAACTACAGTTTTTCTAAGGTATGACAATGTAAAAATCGTGTACCCAAACAGTACCCTTGCAACTAAGCCCATCAATAACTTCTATCGTAGTCCGGACTCAGGAGATGCTGTTGAGTTCTGTGTCCATATAGCCACTCCAGCAGATAAGATTGCTGTTATGAAGCAAAGAATGATAAGTTACATTGAAAATAAGAAGGAGCACTGGTATCCTGCACCAATGTTCATATTAAAGGAAGTGGAAGAATTAAATAGGATGAGGTTTGCCATATGGCTGTCACATAGAATGAACTTCCAAGACATGGGAGAAAAGTGGGCAAGGAGAGCCCTTTTACTTGAAGAGATAGTACGGATCCTCCAGGAGCTTGACATTGAGTATCGTTTGCGGCCCATTGACATAAACGTCCGTGGCATGCCTCCCACGGCCACCACTCTACCTCCCTCTAATTTCACAGCAACCACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

924

Amino Acids

104.54

Weight (kDa)

9.01

Isoelectric Point (pI)

49.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	740 - 796	1.1e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2096
AasI	GACNNNNNNGTC	1 cut(s)	2705
AatII	GACGTC	3 cut(s)	558, 616, 651
Acc36I	ACCTGC	1 cut(s)	675
AccB1I	GGYRCC	3 cut(s)	233, 293, 641
AccB7I	CCANNNNNTGG	1 cut(s)	2605
AccI	GTMKAC	1 cut(s)	625
AccII	CGCG	4 cut(s)	156, 231, 671, 1823
AccIII	TCCGGA	1 cut(s)	2398
AclWI	GGATC	6 cut(s)	111, 356, 1369, 1652, 2650, 2663
AcoI	YGGCCR	2 cut(s)	455, 2727
AcsI	RAATTY	2 cut(s)	305, 1081
AcuI	CTGAAG	2 cut(s)	81, 725
AcyI	GRCGYC	6 cut(s)	234, 294, 555, 613, 642, 648
AfaI	GTAC	4 cut(s)	863, 2348, 2359, 2652
AflIII	ACRYGT	2 cut(s)	669, 2062
AhdI	GACNNNNNGTC	1 cut(s)	1923
AjiI	CACGTC	2 cut(s)	2063, 2248
AjnI	CCWGG	4 cut(s)	1290, 1852, 1872, 2661
AluBI	AGCT	8 cut(s)	88, 376, 451, 988, 1459, 2161, 2668, 2769
AluI	AGCT	8 cut(s)	88, 376, 451, 988, 1459, 2161, 2668, 2769
Alw21I	GWGCWC	3 cut(s)	662, 2014, 2508
Alw26I	GTCTC	4 cut(s)	557, 1259, 1429, 1931
AlwI	GGATC	6 cut(s)	111, 356, 1369, 1652, 2650, 2663
AlwNI	CAGNNNCTG	2 cut(s)	904, 1759
Ama87I	CYCGRG	2 cut(s)	487, 734
Aor13HI	TCCGGA	1 cut(s)	2398
AoxI	GGCC	9 cut(s)	455, 799, 804, 1156, 1394, 1850, 1938, 2690, 2727
ApeKI	GCWGC	3 cut(s)	75, 1571, 1817
ApoI	RAATTY	2 cut(s)	305, 1081
ArsI	GACNNNNNNTTYG	2 cut(s)	1670, 1702
Asp700I	GAANNNNTTC	2 cut(s)	23, 2274
AspLEI	GCGC	7 cut(s)	134, 188, 236, 296, 588, 644, 1357
AspS9I	GGNCC	8 cut(s)	687, 714, 727, 763, 799, 804, 1938, 2691
AsuC2I	CCSGG	2 cut(s)	238, 803
AsuHPI	GGTGA	6 cut(s)	98, 824, 1294, 1793, 2059, 2119
AvaI	CYCGRG	2 cut(s)	487, 734
AvaII	GGWCC	4 cut(s)	687, 714, 727, 763
BamHI	GGATCC	1 cut(s)	2655
BanI	GGYRCC	3 cut(s)	233, 293, 641
BanII	GRGCYC	1 cut(s)	2632
BarI	GAAGNNNNNNTAC	2 cut(s)	2634, 2666
BauI	CACGAG	1 cut(s)	80
BbsI	GAAGAC	5 cut(s)	335, 347, 713, 1292, 1908
Bbv12I	GWGCWC	3 cut(s)	662, 2014, 2508
BbvI	GCAGC	3 cut(s)	87, 1558, 1804
BccI	CCATC	4 cut(s)	436, 718, 2275, 2384
BceAI	ACGGC	5 cut(s)	119, 348, 470, 652, 2742
BciT130I	CCWGG	4 cut(s)	1292, 1854, 1874, 2663
BciVI	GTATCC	1 cut(s)	2523
BclI	TGATCA	2 cut(s)	1188, 1654
BcnI	CCSGG	2 cut(s)	238, 803
BcoDI	GTCTC	4 cut(s)	557, 1259, 1429, 1931
BfaI	CTAG	2 cut(s)	207, 1848
BfmI	CTRYAG	2 cut(s)	1460, 2310
BfoI	RGCGCY	5 cut(s)	135, 189, 237, 297, 645
BfuAI	ACCTGC	1 cut(s)	675
BfuI	GTATCC	1 cut(s)	2523
Bme1390I	CCNGG	6 cut(s)	238, 803, 1292, 1854, 1874, 2663
Bme18I	GGWCC	4 cut(s)	687, 714, 727, 763
BmeRI	GACNNNNNGTC	1 cut(s)	1923
BmeT110I	CYCGRG	2 cut(s)	487, 734
BmgBI	CACGTC	2 cut(s)	2063, 2248
BmgT120I	GGNCC	8 cut(s)	687, 714, 727, 763, 799, 804, 1938, 2691
BmrFI	CCNGG	6 cut(s)	238, 803, 1292, 1854, 1874, 2663
BmrI	ACTGGG	1 cut(s)	1533
BmsI	GCATC	4 cut(s)	1342, 1741, 2223, 2401
BmuI	ACTGGG	1 cut(s)	1533
BpiI	GAAGAC	5 cut(s)	335, 347, 713, 1292, 1908
BplI	GAGNNNNNCTC	2 cut(s)	481, 513
BpmI	CTGGAG	5 cut(s)	860, 881, 1895, 2427, 2645
BpuEI	CTTGAG	1 cut(s)	1247
BpuMI	CCSGG	2 cut(s)	238, 803
Bsa29I	ATCGAT	2 cut(s)	261, 2257
BsaBI	GATNNNNATC	1 cut(s)	2300
BsaHI	GRCGYC	6 cut(s)	234, 294, 555, 613, 642, 648
BsaI	GGTCTC	1 cut(s)	557
BsaJI	CCNNGG	8 cut(s)	488, 597, 735, 1290, 1397, 1770, 2710, 2724
BsaWI	WCCGGW	2 cut(s)	41, 2398
Bse118I	RCCGGY	3 cut(s)	290, 296, 544
Bse1I	ACTGG	7 cut(s)	255, 529, 538, 898, 1528, 1749, 2513
Bse3DI	GCAATG	3 cut(s)	132, 292, 1846
Bse8I	GATNNNNATC	1 cut(s)	2300
BseAI	TCCGGA	1 cut(s)	2398
BseBI	CCWGG	4 cut(s)	1292, 1854, 1874, 2663
BseCI	ATCGAT	2 cut(s)	261, 2257
BseDI	CCNNGG	8 cut(s)	488, 597, 735, 1290, 1397, 1770, 2710, 2724
BseGI	GGATG	7 cut(s)	798, 1053, 1357, 1429, 1753, 1780, 2565
BseJI	GATNNNNATC	1 cut(s)	2300
BseMI	GCAATG	3 cut(s)	132, 292, 1846
BseMII	CTCAG	7 cut(s)	332, 620, 896, 1507, 1935, 2171, 2418
BseNI	ACTGG	7 cut(s)	255, 529, 538, 898, 1528, 1749, 2513
BseRI	GAGGAG	5 cut(s)	368, 836, 857, 1445, 2306
BseX3I	CGGCCG	1 cut(s)	455
BseXI	GCAGC	3 cut(s)	87, 1558, 1804
BseYI	CCCAGC	1 cut(s)	1469
BsgI	GTGCAG	2 cut(s)	377, 2502
Bsh1236I	CGCG	4 cut(s)	156, 231, 671, 1823
Bsh1285I	CGRYCG	2 cut(s)	151, 458
BshFI	GGCC	9 cut(s)	457, 801, 806, 1158, 1396, 1852, 1940, 2692, 2729
BshNI	GGYRCC	3 cut(s)	233, 293, 641
BshVI	ATCGAT	2 cut(s)	261, 2257
BsiEI	CGRYCG	2 cut(s)	151, 458
BsiHKAI	GWGCWC	3 cut(s)	662, 2014, 2508
BsiHKCI	CYCGRG	2 cut(s)	487, 734
BsiSI	CCGG	9 cut(s)	42, 237, 291, 297, 411, 545, 802, 807, 2399
BslFI	GGGAC	4 cut(s)	469, 749, 758, 1531
BsmAI	GTCTC	4 cut(s)	557, 1259, 1429, 1931
BsmFI	GGGAC	4 cut(s)	469, 749, 758, 1531
BsmI	GAATGC	2 cut(s)	1471, 1505
BsnI	GGCC	9 cut(s)	457, 801, 806, 1158, 1396, 1852, 1940, 2692, 2729
Bso31I	GGTCTC	1 cut(s)	557
BsoBI	CYCGRG	2 cut(s)	487, 734
Bsp1286I	GDGCHC	4 cut(s)	662, 2014, 2508, 2632
Bsp13I	TCCGGA	1 cut(s)	2398
BspANI	GGCC	9 cut(s)	457, 801, 806, 1158, 1396, 1852, 1940, 2692, 2729
BspCNI	CTCAG	7 cut(s)	331, 621, 897, 1506, 1936, 2170, 2417
BspDI	ATCGAT	2 cut(s)	261, 2257
BspEI	TCCGGA	1 cut(s)	2398
BspFNI	CGCG	4 cut(s)	156, 231, 671, 1823
BspMI	ACCTGC	1 cut(s)	675
BspPI	GGATC	6 cut(s)	111, 356, 1369, 1652, 2650, 2663
BspT107I	GGYRCC	3 cut(s)	233, 293, 641
BspTNI	GGTCTC	1 cut(s)	557
BsrDI	GCAATG	3 cut(s)	132, 292, 1846
BsrFI	RCCGGY	3 cut(s)	290, 296, 544
BsrI	ACTGG	7 cut(s)	255, 529, 538, 898, 1528, 1749, 2513
BssAI	RCCGGY	3 cut(s)	290, 296, 544
BssECI	CCNNGG	8 cut(s)	488, 597, 735, 1290, 1397, 1770, 2710, 2724
BssNI	GRCGYC	6 cut(s)	234, 294, 555, 613, 642, 648
BssSI	CACGAG	1 cut(s)	80
BssT1I	CCWWGG	2 cut(s)	1397, 1770
Bst2BI	CACGAG	1 cut(s)	80
Bst2UI	CCWGG	4 cut(s)	1292, 1854, 1874, 2663
Bst4CI	ACNGT	6 cut(s)	369, 1145, 1386, 2203, 2314, 2357
Bst6I	CTCTTC	5 cut(s)	1091, 1422, 1589, 1697, 2637
BstACI	GRCGYC	6 cut(s)	234, 294, 555, 613, 642, 648
BstAPI	GCANNNNNTGC	3 cut(s)	466, 1183, 1817
BstC8I	GCNNGC	8 cut(s)	123, 292, 298, 477, 937, 1308, 1485, 2717
BstDSI	CCRYGG	2 cut(s)	2710, 2724
BstF5I	GGATG	7 cut(s)	798, 1053, 1357, 1429, 1753, 1780, 2565
BstFNI	CGCG	4 cut(s)	156, 231, 671, 1823
BstH2I	RGCGCY	5 cut(s)	135, 189, 237, 297, 645
BstHHI	GCGC	7 cut(s)	134, 188, 236, 296, 588, 644, 1357
BstMAI	GTCTC	4 cut(s)	557, 1259, 1429, 1931
BstMCI	CGRYCG	2 cut(s)	151, 458
BstNI	CCWGG	4 cut(s)	1292, 1854, 1874, 2663
BstNSI	RCATGY	2 cut(s)	1606, 2719
BstSCI	CCNGG	6 cut(s)	236, 801, 1290, 1852, 1872, 2661
BstSFI	CTRYAG	2 cut(s)	1460, 2310
BstUI	CGCG	4 cut(s)	156, 231, 671, 1823
BstV1I	GCAGC	3 cut(s)	87, 1558, 1804
BstV2I	GAAGAC	5 cut(s)	335, 347, 713, 1292, 1908
BstX2I	RGATCY	1 cut(s)	2655
BstXI	CCANNNNNNTGG	2 cut(s)	100, 724
BstYI	RGATCY	1 cut(s)	2655
BstZI	CGGCCG	1 cut(s)	455
Bsu15I	ATCGAT	2 cut(s)	261, 2257
BsuI	GTATCC	1 cut(s)	2523
BsuRI	GGCC	9 cut(s)	457, 801, 806, 1158, 1396, 1852, 1940, 2692, 2729
BsuTUI	ATCGAT	2 cut(s)	261, 2257
BtgI	CCRYGG	2 cut(s)	2710, 2724
BtrI	CACGTC	2 cut(s)	2063, 2248
BtsCI	GGATG	7 cut(s)	798, 1053, 1357, 1429, 1753, 1780, 2565
BtsIMutI	CAGTG	5 cut(s)	884, 905, 1382, 1742, 2506
BveI	ACCTGC	1 cut(s)	675
Cac8I	GCNNGC	8 cut(s)	123, 292, 298, 477, 937, 1308, 1485, 2717
CaiI	CAGNNNCTG	2 cut(s)	904, 1759
CfoI	GCGC	7 cut(s)	134, 188, 236, 296, 588, 644, 1357
Cfr10I	RCCGGY	3 cut(s)	290, 296, 544
Cfr13I	GGNCC	8 cut(s)	687, 714, 727, 763, 799, 804, 1938, 2691
ClaI	ATCGAT	2 cut(s)	261, 2257
CseI	GACGC	1 cut(s)	660
Csp6I	GTAC	4 cut(s)	862, 2347, 2358, 2651
CviAII	CATG	7 cut(s)	430, 1322, 1603, 1894, 1921, 2605, 2716
CviQI	GTAC	4 cut(s)	862, 2347, 2358, 2651
DinI	GGCGCC	3 cut(s)	235, 295, 643
DraI	TTTAAA	1 cut(s)	1914
DrdI	GACNNNNNNGTC	1 cut(s)	2705
DriI	GACNNNNNGTC	1 cut(s)	1923
DseDI	GACNNNNNNGTC	1 cut(s)	2705
EaeI	YGGCCR	2 cut(s)	455, 2727
EagI	CGGCCG	1 cut(s)	455
Eam1104I	CTCTTC	5 cut(s)	1091, 1422, 1589, 1697, 2637
Eam1105I	GACNNNNNGTC	1 cut(s)	1923
EarI	CTCTTC	5 cut(s)	1091, 1422, 1589, 1697, 2637
EciI	GGCGGA	1 cut(s)	494
EclXI	CGGCCG	1 cut(s)	455
Eco130I	CCWWGG	2 cut(s)	1397, 1770
Eco147I	AGGCCT	1 cut(s)	1852
Eco24I	GRGCYC	1 cut(s)	2632
Eco31I	GGTCTC	1 cut(s)	557
Eco47I	GGWCC	4 cut(s)	687, 714, 727, 763
Eco52I	CGGCCG	1 cut(s)	455
Eco57I	CTGAAG	2 cut(s)	81, 725
Eco88I	CYCGRG	2 cut(s)	487, 734
EcoO109I	RGGNCCY	2 cut(s)	763, 1938
EcoRII	CCWGG	4 cut(s)	1290, 1852, 1872, 2661
EcoT14I	CCWWGG	2 cut(s)	1397, 1770
EcoT38I	GRGCYC	1 cut(s)	2632
EgeI	GGCGCC	3 cut(s)	235, 295, 643
EheI	GGCGCC	3 cut(s)	235, 295, 643
ErhI	CCWWGG	2 cut(s)	1397, 1770
FaeI	CATG	7 cut(s)	433, 1325, 1606, 1897, 1924, 2608, 2719
FalI	AAGNNNNNCTT	6 cut(s)	8, 40, 1233, 1265, 2189, 2221
FaqI	GGGAC	4 cut(s)	469, 749, 758, 1531
FatI	CATG	7 cut(s)	429, 1321, 1602, 1893, 1920, 2604, 2715
FauI	CCCGC	4 cut(s)	229, 293, 738, 748
FauNDI	CATATG	1 cut(s)	2573
FbaI	TGATCA	2 cut(s)	1188, 1654
FblI	GTMKAC	1 cut(s)	625
FokI	GGATG	7 cut(s)	805, 1060, 1364, 1436, 1760, 1787, 2572
FriOI	GRGCYC	1 cut(s)	2632
FspBI	CTAG	2 cut(s)	207, 1848
GlaI	GCGC	7 cut(s)	133, 187, 235, 295, 587, 643, 1356
GsaI	CCCAGC	1 cut(s)	1473
GsuI	CTGGAG	5 cut(s)	860, 881, 1895, 2427, 2645
HaeII	RGCGCY	5 cut(s)	135, 189, 237, 297, 645
HaeIII	GGCC	9 cut(s)	457, 801, 806, 1158, 1396, 1852, 1940, 2692, 2729
HapII	CCGG	9 cut(s)	42, 237, 291, 297, 411, 545, 802, 807, 2399
HgaI	GACGC	1 cut(s)	660
HhaI	GCGC	7 cut(s)	134, 188, 236, 296, 588, 644, 1357
Hin1I	GRCGYC	6 cut(s)	234, 294, 555, 613, 642, 648
Hin1II	CATG	7 cut(s)	433, 1325, 1606, 1897, 1924, 2608, 2719
Hin6I	GCGC	7 cut(s)	132, 186, 234, 294, 586, 642, 1355
HinP1I	GCGC	7 cut(s)	132, 186, 234, 294, 586, 642, 1355
HincII	GTYRAC	1 cut(s)	626
HindII	GTYRAC	1 cut(s)	626
HpaII	CCGG	9 cut(s)	42, 237, 291, 297, 411, 545, 802, 807, 2399
HphI	GGTGA	6 cut(s)	98, 824, 1294, 1793, 2059, 2119
Hpy166II	GTNNAC	5 cut(s)	47, 626, 2050, 2071, 2347
Hpy8I	GTNNAC	5 cut(s)	47, 626, 2050, 2071, 2347
HpyCH4III	ACNGT	6 cut(s)	369, 1145, 1386, 2203, 2314, 2357
HpyCH4IV	ACGT	8 cut(s)	555, 613, 648, 1967, 2062, 2073, 2247, 2706
HpySE526I	ACGT	8 cut(s)	555, 613, 648, 1967, 2062, 2073, 2247, 2706
Hsp92I	GRCGYC	6 cut(s)	234, 294, 555, 613, 642, 648
Hsp92II	CATG	7 cut(s)	433, 1325, 1606, 1897, 1924, 2608, 2719
HspAI	GCGC	7 cut(s)	132, 186, 234, 294, 586, 642, 1355
KasI	GGCGCC	3 cut(s)	233, 293, 641
KflI	GGGWCCC	1 cut(s)	763
Kpn2I	TCCGGA	1 cut(s)	2398
KroI	GCCGGC	2 cut(s)	290, 296
KroNI	GCCGGC	2 cut(s)	292, 298
Ksp22I	TGATCA	2 cut(s)	1188, 1654
LmnI	GCTCC	7 cut(s)	44, 93, 282, 900, 1862, 2503, 2665
Lsp1109I	GCAGC	3 cut(s)	87, 1558, 1804
LweI	GCATC	4 cut(s)	1342, 1741, 2223, 2401
MaeI	CTAG	2 cut(s)	207, 1848
MaeII	ACGT	8 cut(s)	555, 613, 648, 1967, 2062, 2073, 2247, 2706
MaeIII	GTNAC	7 cut(s)	632, 812, 1234, 2125, 2186, 2483, 2580
MfeI	CAATTG	2 cut(s)	1650, 1990
MflI	RGATCY	1 cut(s)	2655
MhlI	GDGCHC	4 cut(s)	662, 2014, 2508, 2632
MluI	ACGCGT	1 cut(s)	669
Mly113I	GGCGCC	3 cut(s)	234, 294, 642
MlyI	GAGTC	9 cut(s)	260, 410, 491, 578, 587, 883, 1933, 2017, 2396
MmeI	TCCRAC	3 cut(s)	171, 531, 1025
MreI	CGCCGGCG	1 cut(s)	296
MroI	TCCGGA	1 cut(s)	2398
MroNI	GCCGGC	2 cut(s)	290, 296
MroXI	GAANNNNTTC	2 cut(s)	23, 2274
MseI	TTAA	6 cut(s)	678, 1362, 1913, 2306, 2534, 2552
MslI	CAYNNNNRTG	1 cut(s)	98
MspA1I	CMGCKG	2 cut(s)	475, 2045
MspI	CCGG	9 cut(s)	42, 237, 291, 297, 411, 545, 802, 807, 2399
MspR9I	CCNGG	6 cut(s)	238, 803, 1292, 1854, 1874, 2663
MunI	CAATTG	2 cut(s)	1650, 1990
Mva1269I	GAATGC	2 cut(s)	1471, 1505
MvaI	CCWGG	4 cut(s)	1292, 1854, 1874, 2663
MvnI	CGCG	4 cut(s)	156, 231, 671, 1823
NaeI	GCCGGC	2 cut(s)	292, 298
NarI	GGCGCC	3 cut(s)	234, 294, 642
NciI	CCSGG	2 cut(s)	238, 803
NdeI	CATATG	1 cut(s)	2573
NgoMIV	GCCGGC	2 cut(s)	290, 296
NlaIII	CATG	7 cut(s)	433, 1325, 1606, 1897, 1924, 2608, 2719
NmeAIII	GCCGAG	2 cut(s)	15, 586
NmuCI	GTSAC	5 cut(s)	632, 812, 1234, 2125, 2580
NspI	RCATGY	2 cut(s)	1606, 2719
PaeI	GCATGC	1 cut(s)	2719
PceI	AGGCCT	1 cut(s)	1852
PcsI	WCGNNNNNNNCGW	8 cut(s)	144, 171, 530, 564, 622, 642, 668, 671
PctI	GAATGC	2 cut(s)	1471, 1505
PdiI	GCCGGC	2 cut(s)	292, 298
PdmI	GAANNNNTTC	2 cut(s)	23, 2274
PfeI	GAWTC	9 cut(s)	140, 574, 970, 1122, 1221, 1345, 1756, 1763, 1956
PflFI	GACNNNGTC	2 cut(s)	568, 712
PflMI	CCANNNNNTGG	1 cut(s)	2605
PfoI	TCCNGGA	1 cut(s)	2661
PleI	GAGTC	9 cut(s)	259, 410, 490, 577, 587, 883, 1932, 2017, 2396
PluTI	GGCGCC	3 cut(s)	237, 297, 645
PpsI	GAGTC	9 cut(s)	259, 410, 490, 577, 587, 883, 1932, 2017, 2396
PpuMI	RGGWCCY	1 cut(s)	763
PsiI	TTATAA	1 cut(s)	2096
Psp5II	RGGWCCY	1 cut(s)	763
Psp6I	CCWGG	4 cut(s)	1290, 1852, 1872, 2661
PspFI	CCCAGC	1 cut(s)	1469
PspGI	CCWGG	4 cut(s)	1290, 1852, 1872, 2661
PspPI	GGNCC	8 cut(s)	687, 714, 727, 763, 799, 804, 1938, 2691
PspPPI	RGGWCCY	1 cut(s)	763
PstNI	CAGNNNCTG	2 cut(s)	904, 1759
PsuI	RGATCY	1 cut(s)	2655
PsyI	GACNNNGTC	2 cut(s)	568, 712
RsaI	GTAC	4 cut(s)	863, 2348, 2359, 2652
RsaNI	GTAC	4 cut(s)	862, 2347, 2358, 2651
RseI	CAYNNNNRTG	1 cut(s)	98
SalI	GTCGAC	1 cut(s)	624
SaqAI	TTAA	6 cut(s)	678, 1362, 1913, 2306, 2534, 2552
Sau96I	GGNCC	8 cut(s)	687, 714, 727, 763, 799, 804, 1938, 2691
SchI	GAGTC	9 cut(s)	260, 410, 491, 578, 587, 883, 1933, 2017, 2396
ScrFI	CCNGG	6 cut(s)	238, 803, 1292, 1854, 1874, 2663
SduI	GDGCHC	4 cut(s)	662, 2014, 2508, 2632
SfaNI	GCATC	4 cut(s)	1342, 1741, 2223, 2401
SfcI	CTRYAG	2 cut(s)	1460, 2310
SfoI	GGCGCC	3 cut(s)	235, 295, 643
SgrAI	CRCCGGYG	2 cut(s)	296, 544
SinI	GGWCC	4 cut(s)	687, 714, 727, 763
SmiMI	CAYNNNNRTG	1 cut(s)	98
SmlI	CTYRAG	2 cut(s)	14, 1226
SmoI	CTYRAG	2 cut(s)	14, 1226
SphI	GCATGC	1 cut(s)	2719
SseBI	AGGCCT	1 cut(s)	1852
SspDI	GGCGCC	3 cut(s)	233, 293, 641
SspI	AATATT	1 cut(s)	2140
SspMI	CTAG	2 cut(s)	207, 1848
StuI	AGGCCT	1 cut(s)	1852
StyD4I	CCNGG	6 cut(s)	236, 801, 1290, 1852, 1872, 2661
StyI	CCWWGG	2 cut(s)	1397, 1770
TaaI	ACNGT	6 cut(s)	369, 1145, 1386, 2203, 2314, 2357
TaiI	ACGT	8 cut(s)	558, 616, 651, 1970, 2065, 2076, 2250, 2709
TaqII	GACCGA	1 cut(s)	581
TatI	WGTACW	1 cut(s)	861
TfiI	GAWTC	9 cut(s)	140, 574, 970, 1122, 1221, 1345, 1756, 1763, 1956
Tru1I	TTAA	6 cut(s)	678, 1362, 1913, 2306, 2534, 2552
Tru9I	TTAA	6 cut(s)	678, 1362, 1913, 2306, 2534, 2552
TscAI	CASTG	5 cut(s)	891, 905, 1389, 1749, 2513
TseFI	GTSAC	5 cut(s)	632, 812, 1234, 2125, 2580
TseI	GCWGC	3 cut(s)	75, 1571, 1817
Tsp45I	GTSAC	5 cut(s)	632, 812, 1234, 2125, 2580
TspDTI	ATGAA	9 cut(s)	1075, 1440, 1715, 1727, 1882, 2312, 2480, 2518, 2606
TspGWI	ACGGA	4 cut(s)	359, 2020, 2668, 2699
TspRI	CASTG	5 cut(s)	891, 905, 1389, 1749, 2513
Tth111I	GACNNNGTC	2 cut(s)	568, 712
Van91I	CCANNNNNTGG	1 cut(s)	2605
VpaK11BI	GGWCC	4 cut(s)	687, 714, 727, 763
XapI	RAATTY	2 cut(s)	305, 1081
XceI	RCATGY	2 cut(s)	1606, 2719
XmiI	GTMKAC	1 cut(s)	625
XmnI	GAANNNNTTC	2 cut(s)	23, 2274
XspI	CTAG	2 cut(s)	207, 1848
ZraI	GACGTC	3 cut(s)	556, 614, 649

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.