MD08G1006900.v1.1

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001376
Physical Location & Seq
Reverse (-)
547108 .. 550399
3292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1006900.v1.1.491

Sequence Viewer

Length: 2277 bp
ATGGCAACGAGGGAAGGAGAAGTGGTAGTCAAGATCGACGATAGCGACGGTTCTTGTAGCACAGAAACAGACACTGCACCGTATAACGGAGAACCAGCTCCAAGGACACCAGTAATTCAGGGCTCCCCAGAAAAACTGACTGAATCTTTCCGACGGAGGAGCAAAGAGCTTCCGGAAGCTCCCTCCAGCTCCGACGGAGCTGAGGCTGTCATCCAATGCAGCTCCAACGCCTCCTTCTCCCGAAGCCCCTGGAAACCCCCCATGAGCAAGACAAAGTCCAGGCTTCTCGACCTACCAGAGGAAGTGTCTCTAAAATCCGACAGAACGGCGGCTTCCGGACGATCGCATGGAAAAGACGACGACGCTTCGGCTTTCGACGACCCCGACCTTGAAGACATTCCAGAGGAGTACAGGAAGTGGAAGTTCAGCGCGCTCACGTTGCTTCAGTGGGTGAGTCTTGTTATTGTTATAGCTGCTTTGGTTTGCAATCTTTGGATTCCGATTTTAAAGAAGCAAACTTTGTGGGATCTTCCGCTGTGGAAGTGGGAAATTCTGGTTCTGGCATTGATTTGTGGTCGTTTGGTTTCTGGTTGGGGAATTCGGGTGGTTGTGTTCTTCGTAGAGCGAAATTTCTTGTTGCGAAAACGGGTTTTGTATTTCGTTTATGGATTAAGGAAATCGGTGCAGAATTGTCTCTGGTTGGGTTTGGTCTTGTTGGCGTGGCACTTGATATTTGATAAGAAGGTGCAGGAGAAGACCAAGAGTAGGATTTTGCCTTATGTGACCAAGATTCTTGTGTGTTTTCTGGTTGGGACATTGATTTGGCTGCTGAAGACGATACTTGTTAAGGTTCTTGCCTTGTCTTTCCATGTCAACGCATTTTTCGATCGAATTCAGGAGGCTTTGTTCAATCAGTATGTGTTGGAGACACTTTCAGGCCCCCCTATGTTTGAAAGGCGACCGTCACTAGAAGAGGAAATAGCCGCGGCAGCTGAGATTCAGGAGTTTCAGAAAGCGGGGGCTGCTATGCCTCGTGATCTCAGGGCAACTCTTCTTCACGGGGCAAGGAGTGGGAGAGTTGTAGGGAGTGGACACCAAAATAGCCCCAGAGTAGGGAAGAGTCCCACGGTTTCTAGGCCAGCATCTGGGAAGCAAGATGAAGGCATCCCGGTTGATCACTTGCATAAGCTAAACCAAAAGAATGTGTCGGCTTGGAATATGAGAAAAATGGTAAACATCATCCGGCACGGGGCCTTAACCACTCTAGATGAACAGATACTGGACTCTGATATGGGAGACAACTCTTCTTTGATGATTACAACTGAATCTCAAGCAAAAGATGCAGCCAGGAAGATATTTTTCAGAGTAGCCAAGCCGGGGTCTCCATACATTTACCTCAAGGATTTGATGCAGTTTTTGGTTAAAGATGAAGCTTTAAAGACATTTCGTCTCTTTGGTGCAGCAAGTGAAGATGACAAAATCAGCAAGACCGCTCTTAGGAATTGGGTGGTCAATGCATTTAAAGACAGAAGAGCACTAGCATTTTCTTTGAACGATACAAAAACAGCTGTGGATGAACTCCATAACATTTTAAACGTCCTTGTAACTATCGTCATTTTGATAATCTGGCTTATTATACTTGATGTTCATTTCACCCACTTCCTTCTCTTAATGAGCTCCCAGCTTCTTTTGGTTGTATTTATCTTCGGGAACACATGCAAGACTGTTTTTGAAGCAATCATCTTTCTATTTGTAATGCACCCGTTTGATGTGGGTGATCGTTGTGAAGTGGATGGAGTCCAGATGGTAGTTGAAGAAATGAATATATTAACTACGGTCTTTCTGAAGTTTGATAACCACAAGATCATATACCCTAACAGTGTTCTTGCTACCAAGGCAATTGCTAACTATCGTCGTAGTCCTGACATGGGCGATGCTATTGATTTTTGTGTGCATATTTCAACTCCTTTGGAGAGGGTGGCTACTATGAAAGGAAGAATACAGAGGTATATTGAAGACAAGAAGGACCACTGGTACGACGCTCCAATGTTGATCATGAGGGATGTGGAGGAGTTGAACAAACTGAAGATATCGGTATGGCTGACACACAAGATGAACCATCAGGACATGCTGGAGAGATGGCACAGGAGGAGCCTTTTGATCGAAGAGATGATCAAAGTGTTCAGAGAGCTAGATATCGAGTACCGGTTACTGCCTCTCGATGTCAACATCCGAGCCATGCCAAGTTCAACGTCCAACAAGTTTCCTTCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0016020 GO:0044464 GO:0051179 GO:0051234 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel

Protein Analysis

759

Amino Acids

86.37

Weight (kDa)

8.61

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 581 - 639 7.4e-12 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1145
AccBSI CCGCTC 1 cut(s) 1493
AccII CGCG 2 cut(s) 431, 986
AccIII TCCGGA 2 cut(s) 172, 335
AciI CCGC 6 cut(s) 329, 533, 984, 986, 1016, 1491
AclWI GGATC 1 cut(s) 534
AcsI RAATTY 4 cut(s) 549, 597, 628, 891
AcuI CTGAAG 4 cut(s) 428, 851, 1865, 2105
AfaI GTAC 3 cut(s) 410, 2036, 2204
AgeI ACCGGT 1 cut(s) 2205
AhdI GACNNNNNGTC 1 cut(s) 1446
AjnI CCWGG 3 cut(s) 248, 278, 1346
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AloI GAACNNNNNNTCC 2 cut(s) 890, 922
Alw21I GWGCWC 2 cut(s) 1537, 1679
Alw26I GTCTC 6 cut(s) 312, 698, 920, 1290, 1386, 1454
AlwI GGATC 1 cut(s) 534
AlwNI CAGNNNCTG 3 cut(s) 74, 1145, 1279
Aor13HI TCCGGA 2 cut(s) 172, 335
AoxI GGCC 3 cut(s) 937, 1136, 1251
ApeKI GCWGC 7 cut(s) 219, 473, 826, 989, 1022, 1343, 1460
ApoI RAATTY 4 cut(s) 549, 597, 628, 891
AsiGI ACCGGT 1 cut(s) 2205
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 2 cut(s) 431, 433
AspS9I GGNCC 3 cut(s) 938, 1251, 2026
AsuC2I CCSGG 2 cut(s) 1169, 1377
AsuHPI GGTGA 3 cut(s) 463, 1645, 1787
AvaII GGWCC 1 cut(s) 2026
BanII GRGCYC 2 cut(s) 125, 1679
BauI CACGAG 1 cut(s) 1032
BbsI GAAGAC 4 cut(s) 399, 761, 839, 2022
Bbv12I GWGCWC 2 cut(s) 1537, 1679
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1472
BccI CCATC 4 cut(s) 1787, 1798, 2127, 2133
BceAI ACGGC 1 cut(s) 342
BciT130I CCWGG 3 cut(s) 250, 280, 1348
BclI TGATCA 3 cut(s) 1174, 2052, 2172
BcnI CCSGG 2 cut(s) 1169, 1377
BcoDI GTCTC 6 cut(s) 312, 698, 920, 1290, 1386, 1454
BfaI CTAG 5 cut(s) 968, 1134, 1265, 1538, 2192
Bme1390I CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
Bme18I GGWCC 1 cut(s) 2026
BmeRI GACNNNNNGTC 1 cut(s) 1446
BmgT120I GGNCC 3 cut(s) 938, 1251, 2026
BmiI GGNNCC 4 cut(s) 124, 940, 1252, 2153
BmrFI CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
BmsI GCATC 5 cut(s) 1151, 1173, 1330, 1398, 1924
BpiI GAAGAC 4 cut(s) 399, 761, 839, 2022
BpmI CTGGAG 2 cut(s) 169, 2153
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 1314, 1382
BpuMI CCSGG 2 cut(s) 1169, 1377
BsaI GGTCTC 1 cut(s) 1386
BsaJI CCNNGG 6 cut(s) 101, 248, 984, 1125, 1376, 1893
BsaWI WCCGGW 3 cut(s) 172, 335, 2205
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 890, 920
Bse118I RCCGGY 1 cut(s) 2205
Bse1I ACTGG 3 cut(s) 110, 1284, 2036
BseAI TCCGGA 2 cut(s) 172, 335
BseBI CCWGG 3 cut(s) 250, 280, 1348
BseDI CCNNGG 6 cut(s) 101, 248, 984, 1125, 1376, 1893
BseGI GGATG 7 cut(s) 210, 1164, 1239, 1579, 1798, 2068, 2229
BseMII CTCAG 3 cut(s) 192, 984, 1054
BseNI ACTGG 3 cut(s) 110, 1284, 2036
BsePI GCGCGC 1 cut(s) 429
BseRI GAGGAG 4 cut(s) 172, 419, 2084, 2164
BseXI GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1472
BseYI CCCAGC 1 cut(s) 1680
BsgI GTGCAG 4 cut(s) 60, 704, 767, 1479
Bsh1236I CGCG 2 cut(s) 431, 986
Bsh1285I CGRYCG 3 cut(s) 344, 889, 962
BshFI GGCC 3 cut(s) 939, 1138, 1253
BshTI ACCGGT 1 cut(s) 2205
BsiEI CGRYCG 3 cut(s) 344, 889, 962
BsiHKAI GWGCWC 2 cut(s) 1537, 1679
BsiSI CCGG 6 cut(s) 173, 336, 1169, 1243, 1376, 2206
BslFI GGGAC 2 cut(s) 826, 1107
BsmAI GTCTC 6 cut(s) 312, 698, 920, 1290, 1386, 1454
BsmBI CGTCTC 1 cut(s) 1454
BsmFI GGGAC 2 cut(s) 826, 1107
BsnI GGCC 3 cut(s) 939, 1138, 1253
Bso31I GGTCTC 1 cut(s) 1386
Bsp1286I GDGCHC 3 cut(s) 125, 1537, 1679
Bsp13I TCCGGA 2 cut(s) 172, 335
BspACI CCGC 6 cut(s) 329, 533, 984, 986, 1016, 1491
BspANI GGCC 3 cut(s) 939, 1138, 1253
BspCNI CTCAG 3 cut(s) 193, 985, 1053
BspEI TCCGGA 2 cut(s) 172, 335
BspFNI CGCG 2 cut(s) 431, 986
BspHI TCATGA 1 cut(s) 2055
BspLI GGNNCC 4 cut(s) 124, 940, 1252, 2153
BspPI GGATC 1 cut(s) 534
BspQI GCTCTTC 1 cut(s) 1525
BspTNI GGTCTC 1 cut(s) 1386
BsrBI CCGCTC 1 cut(s) 1493
BsrFI RCCGGY 1 cut(s) 2205
BsrI ACTGG 3 cut(s) 110, 1284, 2036
BssAI RCCGGY 1 cut(s) 2205
BssECI CCNNGG 6 cut(s) 101, 248, 984, 1125, 1376, 1893
BssHII GCGCGC 1 cut(s) 429
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 2 cut(s) 101, 1893
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 3 cut(s) 250, 280, 1348
Bst4CI ACNGT 7 cut(s) 50, 81, 963, 1129, 1726, 1837, 1880
Bst6I CTCTTC 6 cut(s) 966, 1056, 1112, 1309, 1525, 2160
BstAPI GCANNNNNTGC 1 cut(s) 1340
BstC8I GCNNGC 2 cut(s) 431, 1140
BstDEI CTNAG 4 cut(s) 201, 993, 1040, 1496
BstDSI CCRYGG 2 cut(s) 984, 1125
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 7 cut(s) 210, 1164, 1239, 1579, 1798, 2068, 2229
BstFNI CGCG 2 cut(s) 431, 986
BstHHI GCGC 2 cut(s) 431, 433
BstMAI GTCTC 6 cut(s) 312, 698, 920, 1290, 1386, 1454
BstMCI CGRYCG 3 cut(s) 344, 889, 962
BstMWI GCNNNNNNNGC 5 cut(s) 439, 989, 1022, 1340, 1895
BstNI CCWGG 3 cut(s) 250, 280, 1348
BstNSI RCATGY 2 cut(s) 1719, 2131
BstSCI CCNGG 5 cut(s) 248, 278, 1167, 1346, 1375
BstUI CGCG 2 cut(s) 431, 986
BstV1I GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1472
BstV2I GAAGAC 4 cut(s) 399, 761, 839, 2022
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BsuRI GGCC 3 cut(s) 939, 1138, 1253
BtgI CCRYGG 2 cut(s) 984, 1125
BtgZI GCGATG 1 cut(s) 1947
BtsCI GGATG 7 cut(s) 210, 1164, 1239, 1579, 1798, 2068, 2229
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 4 cut(s) 72, 452, 1885, 2029
Cac8I GCNNGC 2 cut(s) 431, 1140
CaiI CAGNNNCTG 3 cut(s) 74, 1145, 1279
CciI TCATGA 1 cut(s) 2055
CfoI GCGC 2 cut(s) 431, 433
Cfr10I RCCGGY 1 cut(s) 2205
Cfr13I GGNCC 3 cut(s) 938, 1251, 2026
Cfr42I CCGCGG 1 cut(s) 987
CseI GACGC 2 cut(s) 371, 2048
Csp6I GTAC 3 cut(s) 409, 2035, 2203
CspAI ACCGGT 1 cut(s) 2205
CspCI CAANNNNNGTGG 2 cut(s) 503, 538
CviAII CATG 8 cut(s) 262, 347, 869, 1716, 1927, 2056, 2128, 2239
CviQI GTAC 3 cut(s) 409, 2035, 2203
DdeI CTNAG 4 cut(s) 201, 993, 1040, 1496
DraI TTTAAA 4 cut(s) 507, 1437, 1522, 1593
DriI GACNNNNNGTC 1 cut(s) 1446
Eam1104I CTCTTC 6 cut(s) 966, 1056, 1112, 1309, 1525, 2160
Eam1105I GACNNNNNGTC 1 cut(s) 1446
EarI CTCTTC 6 cut(s) 966, 1056, 1112, 1309, 1525, 2160
Ecl136II GAGCTC 1 cut(s) 1677
Eco130I CCWWGG 2 cut(s) 101, 1893
Eco24I GRGCYC 2 cut(s) 125, 1679
Eco31I GGTCTC 1 cut(s) 1386
Eco32I GATATC 2 cut(s) 2091, 2197
Eco47I GGWCC 1 cut(s) 2026
Eco53kI GAGCTC 1 cut(s) 1677
Eco57I CTGAAG 4 cut(s) 428, 851, 1865, 2105
EcoICRI GAGCTC 1 cut(s) 1677
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoO109I RGGNCCY 2 cut(s) 938, 1251
EcoRI GAATTC 2 cut(s) 597, 891
EcoRII CCWGG 3 cut(s) 248, 278, 1346
EcoRV GATATC 2 cut(s) 2091, 2197
EcoT14I CCWWGG 2 cut(s) 101, 1893
EcoT22I ATGCAT 1 cut(s) 1519
EcoT38I GRGCYC 2 cut(s) 125, 1679
ErhI CCWWGG 2 cut(s) 101, 1893
Esp3I CGTCTC 1 cut(s) 1454
FaeI CATG 8 cut(s) 265, 350, 872, 1719, 1930, 2059, 2131, 2242
FaqI GGGAC 2 cut(s) 826, 1107
FatI CATG 8 cut(s) 261, 346, 868, 1715, 1926, 2055, 2127, 2238
FauI CCCGC 1 cut(s) 1009
FbaI TGATCA 3 cut(s) 1174, 2052, 2172
FokI GGATG 7 cut(s) 197, 1151, 1226, 1586, 1805, 2075, 2216
FriOI GRGCYC 2 cut(s) 125, 1679
FspBI CTAG 5 cut(s) 968, 1134, 1265, 1538, 2192
GlaI GCGC 2 cut(s) 430, 432
GsaI CCCAGC 1 cut(s) 1684
GsuI CTGGAG 2 cut(s) 169, 2153
HaeIII GGCC 3 cut(s) 939, 1138, 1253
HapII CCGG 6 cut(s) 173, 336, 1169, 1243, 1376, 2206
HgaI GACGC 2 cut(s) 371, 2048
HhaI GCGC 2 cut(s) 431, 433
Hin1II CATG 8 cut(s) 265, 350, 872, 1719, 1930, 2059, 2131, 2242
Hin6I GCGC 2 cut(s) 429, 431
HinP1I GCGC 2 cut(s) 429, 431
HincII GTYRAC 2 cut(s) 874, 2227
HindII GTYRAC 2 cut(s) 874, 2227
HindIII AAGCTT 1 cut(s) 1431
HinfI GANTC 9 cut(s) 143, 454, 496, 790, 997, 1120, 1283, 1325, 1797
HpaII CCGG 6 cut(s) 173, 336, 1169, 1243, 1376, 2206
HphI GGTGA 3 cut(s) 463, 1645, 1787
Hpy166II GTNNAC 4 cut(s) 874, 1091, 1234, 2227
Hpy8I GTNNAC 4 cut(s) 874, 1091, 1234, 2227
Hpy99I CGWCG 9 cut(s) 41, 50, 156, 197, 362, 365, 380, 1917, 2042
HpyAV CCTTC 7 cut(s) 8, 244, 736, 1154, 1673, 2017, 2277
HpyCH4III ACNGT 7 cut(s) 50, 81, 963, 1129, 1726, 1837, 1880
HpyCH4IV ACGT 3 cut(s) 437, 1596, 2252
HpyF10VI GCNNNNNNNGC 5 cut(s) 439, 989, 1022, 1340, 1895
HpyF3I CTNAG 4 cut(s) 201, 993, 1040, 1496
HpySE526I ACGT 3 cut(s) 437, 1596, 2252
Hsp92II CATG 8 cut(s) 265, 350, 872, 1719, 1930, 2059, 2131, 2242
HspAI GCGC 2 cut(s) 429, 431
Kpn2I TCCGGA 2 cut(s) 172, 335
Ksp22I TGATCA 3 cut(s) 1174, 2052, 2172
KspI CCGCGG 1 cut(s) 987
LguI GCTCTTC 1 cut(s) 1525
Lsp1109I GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1472
LweI GCATC 5 cut(s) 1151, 1173, 1330, 1398, 1924
MaeI CTAG 5 cut(s) 968, 1134, 1265, 1538, 2192
MaeII ACGT 3 cut(s) 437, 1596, 2252
MaeIII GTNAC 4 cut(s) 781, 963, 1603, 2208
MbiI CCGCTC 1 cut(s) 1493
MfeI CAATTG 1 cut(s) 1899
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 3 cut(s) 125, 1537, 1679
MluCI AATT 9 cut(s) 114, 549, 597, 628, 688, 891, 1501, 1899, 2272
MlyI GAGTC 4 cut(s) 463, 1129, 1277, 1806
MmeI TCCRAC 5 cut(s) 175, 216, 249, 342, 903
Mph1103I ATGCAT 1 cut(s) 1519
MroI TCCGGA 2 cut(s) 172, 335
MroXI GAANNNNTTC 1 cut(s) 396
MspA1I CMGCKG 4 cut(s) 535, 986, 992, 1568
MspI CCGG 6 cut(s) 173, 336, 1169, 1243, 1376, 2206
MspR9I CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
MunI CAATTG 1 cut(s) 1899
MvaI CCWGG 3 cut(s) 250, 280, 1348
MvnI CGCG 2 cut(s) 431, 986
MwoI GCNNNNNNNGC 5 cut(s) 439, 989, 1022, 1340, 1895
NciI CCSGG 2 cut(s) 1169, 1377
NlaIII CATG 8 cut(s) 265, 350, 872, 1719, 1930, 2059, 2131, 2242
NlaIV GGNNCC 4 cut(s) 124, 940, 1252, 2153
NmuCI GTSAC 2 cut(s) 781, 963
NsiI ATGCAT 1 cut(s) 1519
NspI RCATGY 2 cut(s) 1719, 2131
PagI TCATGA 1 cut(s) 2055
PauI GCGCGC 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 1525
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 381, 882
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 5 cut(s) 143, 496, 790, 997, 1325
PflFI GACNNNGTC 1 cut(s) 274
PflMI CCANNNNNTGG 1 cut(s) 1145
PinAI ACCGGT 1 cut(s) 2205
Ple19I CGATCG 2 cut(s) 344, 889
PleI GAGTC 4 cut(s) 462, 1128, 1277, 1805
PpsI GAGTC 4 cut(s) 462, 1128, 1277, 1805
Psp124BI GAGCTC 1 cut(s) 1679
Psp6I CCWGG 3 cut(s) 248, 278, 1346
PspFI CCCAGC 1 cut(s) 1680
PspGI CCWGG 3 cut(s) 248, 278, 1346
PspN4I GGNNCC 4 cut(s) 124, 940, 1252, 2153
PspPI GGNCC 3 cut(s) 938, 1251, 2026
PstNI CAGNNNCTG 3 cut(s) 74, 1145, 1279
PsuI RGATCY 1 cut(s) 526
PsyI GACNNNGTC 1 cut(s) 274
PteI GCGCGC 1 cut(s) 429
PvuI CGATCG 2 cut(s) 344, 889
PvuII CAGCTG 2 cut(s) 992, 1568
RsaI GTAC 3 cut(s) 410, 2036, 2204
RsaNI GTAC 3 cut(s) 409, 2035, 2203
SacI GAGCTC 1 cut(s) 1679
SacII CCGCGG 1 cut(s) 987
SapI GCTCTTC 1 cut(s) 1525
Sau96I GGNCC 3 cut(s) 938, 1251, 2026
SchI GAGTC 4 cut(s) 463, 1129, 1277, 1806
ScrFI CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
SduI GDGCHC 3 cut(s) 125, 1537, 1679
SfaNI GCATC 5 cut(s) 1151, 1173, 1330, 1398, 1924
Sfr303I CCGCGG 1 cut(s) 987
SgrBI CCGCGG 1 cut(s) 987
SinI GGWCC 1 cut(s) 2026
SmlI CTYRAG 2 cut(s) 1329, 1397
SmoI CTYRAG 2 cut(s) 1329, 1397
Sse9I AATT 9 cut(s) 114, 549, 597, 628, 688, 891, 1501, 1899, 2272
SsiI CCGC 6 cut(s) 329, 533, 984, 986, 1016, 1491
SspMI CTAG 5 cut(s) 968, 1134, 1265, 1538, 2192
SstI GAGCTC 1 cut(s) 1679
StyD4I CCNGG 5 cut(s) 248, 278, 1167, 1346, 1375
StyI CCWWGG 2 cut(s) 101, 1893
TaaI ACNGT 7 cut(s) 50, 81, 963, 1129, 1726, 1837, 1880
TaiI ACGT 3 cut(s) 440, 1599, 2255
TaqI TCGA 8 cut(s) 36, 288, 375, 885, 889, 2163, 2199, 2220
TasI AATT 9 cut(s) 114, 549, 597, 628, 688, 891, 1501, 1899, 2272
TatI WGTACW 1 cut(s) 408
TauI GCSGC 3 cut(s) 332, 986, 989
TfiI GAWTC 5 cut(s) 143, 496, 790, 997, 1325
TscAI CASTG 4 cut(s) 79, 452, 1885, 2036
TseFI GTSAC 2 cut(s) 781, 963
TseI GCWGC 7 cut(s) 219, 473, 826, 989, 1022, 1343, 1460
Tsp45I GTSAC 2 cut(s) 781, 963
TspDTI ATGAA 8 cut(s) 1173, 1284, 1443, 1590, 1637, 1835, 2003, 2129
TspGWI ACGGA 3 cut(s) 102, 169, 210
TspRI CASTG 4 cut(s) 79, 452, 1885, 2036
Tth111I GACNNNGTC 1 cut(s) 274
Van91I CCANNNNNTGG 1 cut(s) 1145
VpaK11BI GGWCC 1 cut(s) 2026
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 4 cut(s) 549, 597, 628, 891
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 2 cut(s) 1719, 2131
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 5 cut(s) 968, 1134, 1265, 1538, 2192
Zsp2I ATGCAT 1 cut(s) 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.