Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Reverse (-)

25004820 .. 25009378

Gene Structure 4559 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1126700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2814 bp

ATGGAGTTCTCCATCAAGAAACCGTTTAAGTCCCATGGCTCTGCCAAGCATATGAGGAAGATCTCCGCCGGAGAAGAAGACATCAGCCACGAGGAGCTCCCCATTCTTCTGGATCACGACCACCGCCACCACACCCAGCCCATGACGGACGTCGATTTGTCCGACCGCCGGGAGGTCATCGTCAAAATCGACGAAGGCGCAGATTCGTCCACAAGCACCAGCGACCCTGCCAAAAATGGCAGGAAGATTTGGCGGCAGTCCAGCATCGATTTCTGGCACGGGGACGGCAACGGCGCCGGCAATGCCGAGAATTTTGATTTCGTGCAGCGTGGGAAGACGGAGGAGGGTACTGGGGAGGATCCGCCGTCGAAGCTGATTGGCCAGTTCCTGCATAAGCAGCGAGCCTCCGGCGACATGACGCTGGATATGGATTTGGAGATGGAGGAGCTGCAGCAGAACGAGCGCGATTTGCCGCCCCTTGCCGAGTCGCCGAGTCCTAGGAACTCGCGCAACTCGAAGGAGCTCAAAGTCTCGTTCCAATCGCCGGCGTCTGACCTCACCGATACGCCGCAAACCGAGTCGGTAAGGAGGCGATACAGGGAGTCCCCCGACGAGGAGCGGCGCCGCACCGAGAGGTTGAGCAACGGACAGGACGACGTCGTCCGGTGCACTTCCAACGCCTCATTCCGCCGAGAGCCTTCGTTCTCGAACAAGTCCGATTTGTTGAGAATAAAGACCAAGTCCAGACTAATCGACCCGCCCGAGGAGCCGGATTTCAAGTCGGGCCGGATCCCGCGGTCGGGACAGATCCCGAAATCGGGTCAGATGAGGTCTGGGTTGCTGAGCCGCGGCGGCGACGACGACGACGACGACCCGTTTCTGGAGGAGGACGTGCCGCACGAGTACAAGAGGGCGAACTTCAACGCATTGACGCTGCTTCAGTGGGTAAGCTTGGTTTTGATCATTGGGGCAGTGGTTTGCACGCTGACGATTCCAGTTCTGAGGGAGAAGAGCTTGTGGAAACTGAAATTGTGGAAGTGGGAGGTCTTGATTTTGGTTCTGATATGCGGGAGATTGGTTTCGGGTTGGGGGATTAGGATCATAGTGTTTTTTGTGGAGAGGAATTTCCTTCTGCGGAAGAGAGTTTTGTATTTCGTGTATGGAGTCCGAAAGGCGGTGCAGAACTGCCTCTGGCTCGGCCTGGTTCTTTTAGCATGGCATTTCATGTTTGATAAGAAGGTGGAGAGGGAGACCAATACCGAGGTGGTTGCTTATGTGACGAAAATCTTGTTTTGTCTCTTGATTGGAGTGTTGCTGTGGCTGGTGAAGACGTTGATTGTGAAGGTGCTGGCTTCGTCGTTCCATGTGAGGTCGTATTTTGATCGGATTCAGGACTCTCTTTTTAATCAGTATGTGATTGAGACGCTGTCCGGGCGGCCTTTGATTGAGATGCAGAATGCGGAGGAGGAGGAGGATAGGCTGGCGGATGAGGTCAGGAAGCTGCAGAATGCCGGGGCTACTATGCCTCCTGACCTCAAGGCAAATGCATTCCCGTCCGCTAGGATTGGGAGGGTGATTGGGAGTGGTTCGATAAGGAGTGGGAGGGTGATTGCGGGTAGCGGGCTTATTGGGAAGAGCACCAAGTATTCTAGGCCACTTTCCAAGAGGGCAGAGGAGCCCGGGATCACGATTGATCATTTGCATAAGCTCAATCCCAAGAATGTGTCTGCTTGGAATATGAAGAGGCTGATGAATATAGTTAGAAAAGGGCATCTTACGACGCTTGATGAGCAGATTCTGGATGCCACTGGTGAGGATGAGGCTGATACTCAGATCAGGAGTGAGGTTGAGGCAAAGGCTGCTGCTAAAAAGATATTCCAGAATGTGGCTAGGCCTGGTTCCAAGTTCATATACCTGGAGGACTTGATGCGTTTCATGGAAGAAGACGAGGCTGTGAAGACCATGAGCCTTTTTGAAGGGGCATCTGAGCACAAGAGAATCAGCAAATCTGCCTTGAAAAATTGGGTGGTCAGTGCCTTCAGAGAACGGAGAGCACTTGCTCTGACGCTGAATGATACCAAAACAGCTGTGAATACACTTCATCGCGTGGTGAACATAATAGTTGCCATTGTTATAATTGTTATATGGCTTCTTGTTCTGGGAATTGCCACCAGCAAATTTCTCTTGTTTGTGAGTTCTCAGCTTGTGGTTGTGGCATTTGTCTTTGGTAACACCTGCAAGACGATATTTGAAGCAATCATATTTTTATTCGTGATGCATCCATTTGACGTGGGAGATCGTTGCGATATAGATGGAGTTCAGATGGTAGTTGAAGAAATGAACATCTTGACTACAGTTTTCCTAAGGTATGACAACACAAAAATCGTGTACCCAAACAGCATCCTAGCGACTTTGCCCATCCTTAACTACTATCGTAGTCCTGACACGGGAGATACTATTGAGTTCTGCATCCATGTATCAACTCCGCCAGATAGGATTGTTTTAATGAGGCAAAGAATAATCAGTTACATCGAGAACAAGAAGGAGCACTGGTATCCAGCACCAATGGTTATAATGAAGGATGTAGAAGAACTAAATAGGGTACGGTTTGCAATTTGGCCTCAACATAGAATGAACTTCCAGGACATAGGAGAAAGGTTTGTAAGGAGAGGCTATTTGGTCGAAGAGATGGTACGTATATTCCAGGAGCTTGACATCCAGTTCCGCCTTCTGCCTGTTGACATAAACGTCCGCGCCATGCCTGGCATGACTGGCCAGGTTCCCTCTAATTACACGGCAACTAAAGCCGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

938

Amino Acids

106.98

Weight (kDa)

7.97

Isoelectric Point (pI)

44.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	754 - 811	1.8e-09	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2135, 2573
AarI	CACCTGC	1 cut(s)	2243
AasI	GACNNNNNNGTC	2 cut(s)	659, 2747
AatII	GACGTC	2 cut(s)	153, 660
Acc36I	ACCTGC	1 cut(s)	2243
AccB1I	GGYRCC	2 cut(s)	293, 621
AccB7I	CCANNNNNTGG	1 cut(s)	1885
AccBSI	CCGCTC	1 cut(s)	619
AccII	CGCG	6 cut(s)	465, 508, 796, 849, 2106, 2754
AclWI	GGATC	8 cut(s)	120, 353, 366, 784, 797, 802, 1106, 1691
AcoI	YGGCCR	2 cut(s)	379, 2773
AcsI	RAATTY	3 cut(s)	310, 1123, 2177
AcuI	CTGAAG	2 cut(s)	923, 2023
AcyI	GRCGYC	5 cut(s)	150, 294, 548, 622, 657
AfaI	GTAC	5 cut(s)	349, 905, 2390, 2604, 2694
AgsI	TTSAA	6 cut(s)	778, 922, 1976, 2017, 2252, 2333
AjiI	CACGTC	2 cut(s)	892, 2290
AjnI	CCWGG	7 cut(s)	1200, 1894, 1914, 2640, 2703, 2761, 2775
AjuI	GAANNNNNNNTTGG	2 cut(s)	668, 700
Alw21I	GWGCWC	7 cut(s)	99, 525, 671, 1640, 1992, 2056, 2550
Alw26I	GTCTC	4 cut(s)	535, 1244, 1301, 1415
Alw44I	GTGCAC	1 cut(s)	667
AlwI	GGATC	8 cut(s)	120, 353, 366, 784, 797, 802, 1106, 1691
AlwNI	CAGNNNCTG	2 cut(s)	388, 1798
Ama87I	CYCGRG	2 cut(s)	761, 1679
AoxI	GGCC	8 cut(s)	379, 784, 1198, 1436, 1652, 1892, 2618, 2773
ApaLI	GTGCAC	1 cut(s)	667
ApeKI	GCWGC	8 cut(s)	325, 397, 448, 451, 934, 1501, 1859, 1862
ApoI	RAATTY	3 cut(s)	310, 1123, 2177
AspA2I	CCTAGG	1 cut(s)	497
AspLEI	GCGC	6 cut(s)	200, 296, 465, 510, 624, 2756
AspS9I	GGNCC	1 cut(s)	784
AsuC2I	CCSGG	5 cut(s)	170, 1432, 1513, 1680, 1681
AsuHPI	GGTGA	6 cut(s)	550, 1336, 1585, 1618, 1823, 2122
AvaI	CYCGRG	2 cut(s)	761, 1679
AvrII	CCTAGG	1 cut(s)	497
AxyI	CCTNAGG	1 cut(s)	2363
BaeGI	GKGCMC	1 cut(s)	671
BalI	TGGCCA	2 cut(s)	381, 2775
BamHI	GGATCC	2 cut(s)	358, 789
BanI	GGYRCC	2 cut(s)	293, 621
BanII	GRGCYC	3 cut(s)	99, 525, 1680
BarI	GAAGNNNNNNTAC	2 cut(s)	2676, 2708
BauI	CACGAG	2 cut(s)	89, 899
BbsI	GAAGAC	5 cut(s)	84, 341, 1334, 1950, 1964
Bbv12I	GWGCWC	7 cut(s)	99, 525, 671, 1640, 1992, 2056, 2550
BbvI	GCAGC	8 cut(s)	337, 409, 435, 463, 921, 1488, 1846, 1849
BccI	CCATC	6 cut(s)	20, 433, 2306, 2317, 2426, 2683
BceAI	ACGGC	4 cut(s)	301, 307, 349, 2811
BcgI	CGANNNNNNTGC	2 cut(s)	1250, 1284
BciT130I	CCWGG	7 cut(s)	1202, 1896, 1916, 2642, 2705, 2763, 2777
BciVI	GTATCC	1 cut(s)	2565
BclI	TGATCA	2 cut(s)	960, 1693
BcnI	CCSGG	5 cut(s)	170, 1432, 1513, 1680, 1681
BcoDI	GTCTC	4 cut(s)	535, 1244, 1301, 1415
BfaI	CTAG	5 cut(s)	498, 1560, 1650, 1890, 2405
BfmI	CTRYAG	3 cut(s)	449, 1502, 2352
BfoI	RGCGCY	2 cut(s)	297, 625
BfuAI	ACCTGC	1 cut(s)	2243
BfuI	GTATCC	1 cut(s)	2565
BglI	GCCNNNNNGGC	1 cut(s)	852
BglII	AGATCT	1 cut(s)	60
BlnI	CCTAGG	1 cut(s)	497
BlpI	GCTNAGC	1 cut(s)	842
BmeT110I	CYCGRG	2 cut(s)	761, 1679
BmgBI	CACGTC	2 cut(s)	892, 2290
BmgT120I	GGNCC	1 cut(s)	784
BmiI	GGNNCC	8 cut(s)	295, 360, 623, 768, 791, 1677, 1900, 2781
BmrI	ACTGGG	1 cut(s)	360
BmuI	ACTGGG	1 cut(s)	360
BoxI	GACNNNNGTC	1 cut(s)	149
BpiI	GAAGAC	5 cut(s)	84, 341, 1334, 1950, 1964
BpmI	CTGGAG	2 cut(s)	902, 1937
Bpu1102I	GCTNAGC	1 cut(s)	842
BpuEI	CTTGAG	1 cut(s)	1520
BpuMI	CCSGG	5 cut(s)	170, 1432, 1513, 1680, 1681
Bsa29I	ATCGAT	1 cut(s)	267
BsaAI	YACGTR	1 cut(s)	2696
BsaBI	GATNNNNATC	1 cut(s)	1097
BsaHI	GRCGYC	5 cut(s)	150, 294, 548, 622, 657
BsaI	GGTCTC	1 cut(s)	1244
BsaJI	CCNNGG	8 cut(s)	34, 497, 762, 794, 847, 1260, 1512, 1679
BsaWI	WCCGGW	1 cut(s)	663
BsaXI	ACNNNNNCTCC	4 cut(s)	1510, 1540, 2537, 2567
Bse118I	RCCGGY	2 cut(s)	296, 544
Bse1I	ACTGG	7 cut(s)	355, 382, 995, 1813, 2555, 2719, 2776
Bse21I	CCTNAGG	1 cut(s)	2363
Bse3DI	GCAATG	1 cut(s)	307
Bse8I	GATNNNNATC	1 cut(s)	1097
BseBI	CCWGG	7 cut(s)	1202, 1896, 1916, 2642, 2705, 2763, 2777
BseCI	ATCGAT	1 cut(s)	267
BseDI	CCNNGG	8 cut(s)	34, 497, 762, 794, 847, 1260, 1512, 1679
BseGI	GGATG	9 cut(s)	1492, 1807, 1822, 2278, 2400, 2418, 2469, 2587, 2715
BseJI	GATNNNNATC	1 cut(s)	1097
BseMI	GCAATG	1 cut(s)	307
BseMII	CTCAG	5 cut(s)	833, 992, 1844, 1977, 2213
BseNI	ACTGG	7 cut(s)	355, 382, 995, 1813, 2555, 2719, 2776
BseSI	GKGCMC	1 cut(s)	671
BseXI	GCAGC	8 cut(s)	337, 409, 435, 463, 921, 1488, 1846, 1849
BseYI	CCCAGC	1 cut(s)	135
BsgI	GTGCAG	2 cut(s)	344, 1199
Bsh1236I	CGCG	6 cut(s)	465, 508, 796, 849, 2106, 2754
Bsh1285I	CGRYCG	2 cut(s)	166, 800
BshFI	GGCC	8 cut(s)	381, 786, 1200, 1438, 1654, 1894, 2620, 2775
BshNI	GGYRCC	2 cut(s)	293, 621
BshVI	ATCGAT	1 cut(s)	267
BsiEI	CGRYCG	2 cut(s)	166, 800
BsiHKAI	GWGCWC	7 cut(s)	99, 525, 671, 1640, 1992, 2056, 2550
BsiHKCI	CYCGRG	2 cut(s)	761, 1679
BslFI	GGGAC	4 cut(s)	16, 296, 589, 816
BsmAI	GTCTC	4 cut(s)	535, 1244, 1301, 1415
BsmBI	CGTCTC	1 cut(s)	1415
BsmFI	GGGAC	4 cut(s)	16, 296, 589, 816
BsmI	GAATGC	3 cut(s)	1462, 1513, 1547
BsnI	GGCC	8 cut(s)	381, 786, 1200, 1438, 1654, 1894, 2620, 2775
Bso31I	GGTCTC	1 cut(s)	1244
BsoBI	CYCGRG	2 cut(s)	761, 1679
Bsp1286I	GDGCHC	8 cut(s)	99, 525, 671, 1640, 1680, 1992, 2056, 2550
Bsp1720I	GCTNAGC	1 cut(s)	842
Bsp19I	CCATGG	1 cut(s)	34
BspANI	GGCC	8 cut(s)	381, 786, 1200, 1438, 1654, 1894, 2620, 2775
BspCNI	CTCAG	5 cut(s)	834, 993, 1843, 1978, 2212
BspDI	ATCGAT	1 cut(s)	267
BspFNI	CGCG	6 cut(s)	465, 508, 796, 849, 2106, 2754
BspLI	GGNNCC	8 cut(s)	295, 360, 623, 768, 791, 1677, 1900, 2781
BspMAI	CTGCAG	2 cut(s)	453, 1506
BspMI	ACCTGC	1 cut(s)	2243
BspPI	GGATC	8 cut(s)	120, 353, 366, 784, 797, 802, 1106, 1691
BspQI	GCTCTTC	2 cut(s)	1004, 1628
BspT107I	GGYRCC	2 cut(s)	293, 621
BspTNI	GGTCTC	1 cut(s)	1244
BsrBI	CCGCTC	1 cut(s)	619
BsrDI	GCAATG	1 cut(s)	307
BsrFI	RCCGGY	2 cut(s)	296, 544
BsrI	ACTGG	7 cut(s)	355, 382, 995, 1813, 2555, 2719, 2776
BssAI	RCCGGY	2 cut(s)	296, 544
BssECI	CCNNGG	8 cut(s)	34, 497, 762, 794, 847, 1260, 1512, 1679
BssNI	GRCGYC	5 cut(s)	150, 294, 548, 622, 657
BssSI	CACGAG	2 cut(s)	89, 899
BssT1I	CCWWGG	2 cut(s)	34, 497
Bst2BI	CACGAG	2 cut(s)	89, 899
Bst2UI	CCWGG	7 cut(s)	1202, 1896, 1916, 2642, 2705, 2763, 2777
Bst4CI	ACNGT	3 cut(s)	24, 2356, 2607
Bst6I	CTCTTC	5 cut(s)	1004, 1133, 1628, 1736, 2679
BstACI	GRCGYC	5 cut(s)	150, 294, 548, 622, 657
BstAPI	GCANNNNNTGC	1 cut(s)	1859
BstBAI	YACGTR	1 cut(s)	2696
BstC8I	GCNNGC	7 cut(s)	298, 402, 546, 983, 1350, 1482, 1622
BstDEI	CTNAG	6 cut(s)	842, 1001, 1830, 1986, 2199, 2363
BstDSI	CCRYGG	3 cut(s)	34, 794, 847
BstF5I	GGATG	9 cut(s)	1492, 1807, 1822, 2278, 2400, 2418, 2469, 2587, 2715
BstFNI	CGCG	6 cut(s)	465, 508, 796, 849, 2106, 2754
BstH2I	RGCGCY	2 cut(s)	297, 625
BstHHI	GCGC	6 cut(s)	200, 296, 465, 510, 624, 2756
BstMAI	GTCTC	4 cut(s)	535, 1244, 1301, 1415
BstMCI	CGRYCG	2 cut(s)	166, 800
BstNI	CCWGG	7 cut(s)	1202, 1896, 1916, 2642, 2705, 2763, 2777
BstPAI	GACNNNNGTC	1 cut(s)	149
BstSFI	CTRYAG	3 cut(s)	449, 1502, 2352
BstSLI	GKGCMC	1 cut(s)	671
BstSNI	TACGTA	1 cut(s)	2696
BstUI	CGCG	6 cut(s)	465, 508, 796, 849, 2106, 2754
BstV1I	GCAGC	8 cut(s)	337, 409, 435, 463, 921, 1488, 1846, 1849
BstV2I	GAAGAC	5 cut(s)	84, 341, 1334, 1950, 1964
BstX2I	RGATCY	4 cut(s)	60, 358, 789, 807
BstXI	CCANNNNNNTGG	1 cut(s)	109
BstYI	RGATCY	4 cut(s)	60, 358, 789, 807
Bsu15I	ATCGAT	1 cut(s)	267
Bsu36I	CCTNAGG	1 cut(s)	2363
BsuI	GTATCC	1 cut(s)	2565
BsuRI	GGCC	8 cut(s)	381, 786, 1200, 1438, 1654, 1894, 2620, 2775
BsuTUI	ATCGAT	1 cut(s)	267
BtgI	CCRYGG	3 cut(s)	34, 794, 847
BtgZI	GCGATG	1 cut(s)	2087
BtrI	CACGTC	2 cut(s)	892, 2290
BtsCI	GGATG	9 cut(s)	1492, 1807, 1822, 2278, 2400, 2418, 2469, 2587, 2715
BtsI	GCAGTG	1 cut(s)	978
BtsIMutI	CAGTG	5 cut(s)	947, 978, 1806, 2038, 2548
BveI	ACCTGC	1 cut(s)	2243
Cac8I	GCNNGC	7 cut(s)	298, 402, 546, 983, 1350, 1482, 1622
CaiI	CAGNNNCTG	2 cut(s)	388, 1798
CfoI	GCGC	6 cut(s)	200, 296, 465, 510, 624, 2756
Cfr10I	RCCGGY	2 cut(s)	296, 544
Cfr13I	GGNCC	1 cut(s)	784
Cfr42I	CCGCGG	2 cut(s)	797, 850
Cfr9I	CCCGGG	1 cut(s)	1679
ClaI	ATCGAT	1 cut(s)	267
CseI	GACGC	6 cut(s)	427, 537, 940, 1432, 1789, 2074
Csp6I	GTAC	5 cut(s)	348, 904, 2389, 2603, 2693
CviQI	GTAC	5 cut(s)	348, 904, 2389, 2603, 2693
DdeI	CTNAG	6 cut(s)	842, 1001, 1830, 1986, 2199, 2363
DinI	GGCGCC	2 cut(s)	295, 623
DrdI	GACNNNNNNGTC	2 cut(s)	659, 2747
DseDI	GACNNNNNNGTC	2 cut(s)	659, 2747
EaeI	YGGCCR	2 cut(s)	379, 2773
Eam1104I	CTCTTC	5 cut(s)	1004, 1133, 1628, 1736, 2679
EarI	CTCTTC	5 cut(s)	1004, 1133, 1628, 1736, 2679
EciI	GGCGGA	6 cut(s)	55, 351, 677, 1499, 2475, 2714
Ecl136II	GAGCTC	2 cut(s)	97, 523
Eco105I	TACGTA	1 cut(s)	2696
Eco130I	CCWWGG	2 cut(s)	34, 497
Eco147I	AGGCCT	1 cut(s)	1894
Eco24I	GRGCYC	3 cut(s)	99, 525, 1680
Eco31I	GGTCTC	1 cut(s)	1244
Eco53kI	GAGCTC	2 cut(s)	97, 523
Eco57I	CTGAAG	2 cut(s)	923, 2023
Eco81I	CCTNAGG	1 cut(s)	2363
Eco88I	CYCGRG	2 cut(s)	761, 1679
EcoICRI	GAGCTC	2 cut(s)	97, 523
EcoRII	CCWGG	7 cut(s)	1200, 1894, 1914, 2640, 2703, 2761, 2775
EcoT14I	CCWWGG	2 cut(s)	34, 497
EcoT22I	ATGCAT	2 cut(s)	1549, 2280
EcoT38I	GRGCYC	3 cut(s)	99, 525, 1680
EgeI	GGCGCC	2 cut(s)	295, 623
EheI	GGCGCC	2 cut(s)	295, 623
ErhI	CCWWGG	2 cut(s)	34, 497
Esp3I	CGTCTC	1 cut(s)	1415
FalI	AAGNNNNNCTT	2 cut(s)	1758, 1790
FaqI	GGGAC	4 cut(s)	16, 296, 589, 816
FauI	CCCGC	5 cut(s)	765, 801, 1061, 1606, 1613
FauNDI	CATATG	1 cut(s)	51
FbaI	TGATCA	2 cut(s)	960, 1693
FokI	GGATG	9 cut(s)	1499, 1814, 1829, 2265, 2387, 2405, 2456, 2594, 2702
FriOI	GRGCYC	3 cut(s)	99, 525, 1680
FspBI	CTAG	5 cut(s)	498, 1560, 1650, 1890, 2405
GlaI	GCGC	6 cut(s)	199, 295, 464, 509, 623, 2755
GsaI	CCCAGC	1 cut(s)	139
GsuI	CTGGAG	2 cut(s)	902, 1937
HaeII	RGCGCY	2 cut(s)	297, 625
HaeIII	GGCC	8 cut(s)	381, 786, 1200, 1438, 1654, 1894, 2620, 2775
HgaI	GACGC	6 cut(s)	427, 537, 940, 1432, 1789, 2074
HhaI	GCGC	6 cut(s)	200, 296, 465, 510, 624, 2756
Hin1I	GRCGYC	5 cut(s)	150, 294, 548, 622, 657
Hin6I	GCGC	6 cut(s)	198, 294, 463, 508, 622, 2754
HinP1I	GCGC	6 cut(s)	198, 294, 463, 508, 622, 2754
HincII	GTYRAC	1 cut(s)	2740
HindII	GTYRAC	1 cut(s)	2740
HindIII	AAGCTT	1 cut(s)	949
HphI	GGTGA	6 cut(s)	550, 1336, 1585, 1618, 1823, 2122
Hpy166II	GTNNAC	5 cut(s)	210, 669, 2113, 2389, 2740
Hpy8I	GTNNAC	5 cut(s)	210, 669, 2113, 2389, 2740
HpyCH4III	ACNGT	3 cut(s)	24, 2356, 2607
HpyCH4IV	ACGT	7 cut(s)	150, 657, 891, 1331, 2289, 2695, 2748
HpyF3I	CTNAG	6 cut(s)	842, 1001, 1830, 1986, 2199, 2363
HpySE526I	ACGT	7 cut(s)	150, 657, 891, 1331, 2289, 2695, 2748
Hsp92I	GRCGYC	5 cut(s)	150, 294, 548, 622, 657
HspAI	GCGC	6 cut(s)	198, 294, 463, 508, 622, 2754
KasI	GGCGCC	2 cut(s)	293, 621
KroI	GCCGGC	2 cut(s)	296, 544
KroNI	GCCGGC	2 cut(s)	298, 546
Ksp22I	TGATCA	2 cut(s)	960, 1693
KspI	CCGCGG	2 cut(s)	797, 850
LguI	GCTCTTC	2 cut(s)	1004, 1628
LmnI	GCTCC	9 cut(s)	94, 102, 445, 520, 616, 766, 1675, 2545, 2707
Lsp1109I	GCAGC	8 cut(s)	337, 409, 435, 463, 921, 1488, 1846, 1849
MaeI	CTAG	5 cut(s)	498, 1560, 1650, 1890, 2405
MaeII	ACGT	7 cut(s)	150, 657, 891, 1331, 2289, 2695, 2748
MaeIII	GTNAC	3 cut(s)	1276, 2228, 2525
MbiI	CCGCTC	1 cut(s)	619
MflI	RGATCY	4 cut(s)	60, 358, 789, 807
MhlI	GDGCHC	8 cut(s)	99, 525, 671, 1640, 1680, 1992, 2056, 2550
MlsI	TGGCCA	2 cut(s)	381, 2775
MluCI	AATT	9 cut(s)	310, 1028, 1123, 2020, 2136, 2163, 2177, 2613, 2788
MluNI	TGGCCA	2 cut(s)	381, 2775
Mly113I	GGCGCC	2 cut(s)	294, 622
MlyI	GAGTC	6 cut(s)	494, 502, 587, 611, 1173, 1388
MmeI	TCCRAC	2 cut(s)	186, 699
Mox20I	TGGCCA	2 cut(s)	381, 2775
Mph1103I	ATGCAT	2 cut(s)	1549, 2280
MreI	CGCCGGCG	1 cut(s)	544
MroNI	GCCGGC	2 cut(s)	296, 544
MscI	TGGCCA	2 cut(s)	381, 2775
MseI	TTAA	4 cut(s)	27, 1404, 2424, 2504
Msp20I	TGGCCA	2 cut(s)	381, 2775
MspA1I	CMGCKG	3 cut(s)	796, 849, 2087
Mva1269I	GAATGC	3 cut(s)	1462, 1513, 1547
MvaI	CCWGG	7 cut(s)	1202, 1896, 1916, 2642, 2705, 2763, 2777
MvnI	CGCG	6 cut(s)	465, 508, 796, 849, 2106, 2754
NaeI	GCCGGC	2 cut(s)	298, 546
NarI	GGCGCC	2 cut(s)	294, 622
NciI	CCSGG	5 cut(s)	170, 1432, 1513, 1680, 1681
NcoI	CCATGG	1 cut(s)	34
NdeI	CATATG	1 cut(s)	51
NgoMIV	GCCGGC	2 cut(s)	296, 544
NlaIV	GGNNCC	8 cut(s)	295, 360, 623, 768, 791, 1677, 1900, 2781
NmeAIII	GCCGAG	5 cut(s)	331, 508, 516, 716, 1176
NmuCI	GTSAC	1 cut(s)	1276
NsiI	ATGCAT	2 cut(s)	1549, 2280
PaqCI	CACCTGC	1 cut(s)	2243
PceI	AGGCCT	1 cut(s)	1894
PciSI	GCTCTTC	2 cut(s)	1004, 1628
PcsI	WCGNNNNNNNCGW	8 cut(s)	159, 186, 512, 651, 759, 864, 867, 897
PctI	GAATGC	3 cut(s)	1462, 1513, 1547
PdiI	GCCGGC	2 cut(s)	298, 546
PfeI	GAWTC	5 cut(s)	203, 991, 1387, 1795, 1998
PflFI	GACNNNGTC	4 cut(s)	656, 659, 739, 1426
PflMI	CCANNNNNTGG	1 cut(s)	1885
PfoI	TCCNGGA	2 cut(s)	2640, 2703
PleI	GAGTC	6 cut(s)	493, 501, 586, 610, 1172, 1388
PluTI	GGCGCC	2 cut(s)	297, 625
PpsI	GAGTC	6 cut(s)	493, 501, 586, 610, 1172, 1388
Ppu21I	YACGTR	1 cut(s)	2696
PshAI	GACNNNNGTC	1 cut(s)	149
PsiI	TTATAA	2 cut(s)	2135, 2573
Psp124BI	GAGCTC	2 cut(s)	99, 525
Psp6I	CCWGG	7 cut(s)	1200, 1894, 1914, 2640, 2703, 2761, 2775
PspFI	CCCAGC	1 cut(s)	135
PspGI	CCWGG	7 cut(s)	1200, 1894, 1914, 2640, 2703, 2761, 2775
PspN4I	GGNNCC	8 cut(s)	295, 360, 623, 768, 791, 1677, 1900, 2781
PspPI	GGNCC	1 cut(s)	784
PstI	CTGCAG	2 cut(s)	453, 1506
PstNI	CAGNNNCTG	2 cut(s)	388, 1798
PsuI	RGATCY	4 cut(s)	60, 358, 789, 807
PsyI	GACNNNGTC	4 cut(s)	656, 659, 739, 1426
PvuII	CAGCTG	1 cut(s)	2087
RsaI	GTAC	5 cut(s)	349, 905, 2390, 2604, 2694
RsaNI	GTAC	5 cut(s)	348, 904, 2389, 2603, 2693
SacI	GAGCTC	2 cut(s)	99, 525
SacII	CCGCGG	2 cut(s)	797, 850
SapI	GCTCTTC	2 cut(s)	1004, 1628
SaqAI	TTAA	4 cut(s)	27, 1404, 2424, 2504
Sau96I	GGNCC	1 cut(s)	784
SchI	GAGTC	6 cut(s)	494, 502, 587, 611, 1173, 1388
SduI	GDGCHC	8 cut(s)	99, 525, 671, 1640, 1680, 1992, 2056, 2550
SfcI	CTRYAG	3 cut(s)	449, 1502, 2352
SfoI	GGCGCC	2 cut(s)	295, 623
Sfr303I	CCGCGG	2 cut(s)	797, 850
SgrAI	CRCCGGYG	1 cut(s)	544
SgrBI	CCGCGG	2 cut(s)	797, 850
SmaI	CCCGGG	1 cut(s)	1681
SmlI	CTYRAG	1 cut(s)	1535
SmoI	CTYRAG	1 cut(s)	1535
SnaBI	TACGTA	1 cut(s)	2696
Sse9I	AATT	9 cut(s)	310, 1028, 1123, 2020, 2136, 2163, 2177, 2613, 2788
SseBI	AGGCCT	1 cut(s)	1894
SspDI	GGCGCC	2 cut(s)	293, 621
SspMI	CTAG	5 cut(s)	498, 1560, 1650, 1890, 2405
SstI	GAGCTC	2 cut(s)	99, 525
StuI	AGGCCT	1 cut(s)	1894
StyI	CCWWGG	2 cut(s)	34, 497
TaaI	ACNGT	3 cut(s)	24, 2356, 2607
TaiI	ACGT	7 cut(s)	153, 660, 894, 1334, 2292, 2698, 2751
TasI	AATT	9 cut(s)	310, 1028, 1123, 2020, 2136, 2163, 2177, 2613, 2788
TatI	WGTACW	1 cut(s)	903
TfiI	GAWTC	5 cut(s)	203, 991, 1387, 1795, 1998
Tru1I	TTAA	4 cut(s)	27, 1404, 2424, 2504
Tru9I	TTAA	4 cut(s)	27, 1404, 2424, 2504
TscAI	CASTG	5 cut(s)	947, 978, 1813, 2038, 2555
TseFI	GTSAC	1 cut(s)	1276
TseI	GCWGC	8 cut(s)	325, 397, 448, 451, 934, 1501, 1859, 1862
Tsp45I	GTSAC	1 cut(s)	1276
TspDTI	ATGAA	9 cut(s)	1213, 1754, 1766, 1897, 1924, 2090, 2354, 2591, 2648
TspGWI	ACGGA	4 cut(s)	161, 353, 660, 2062
TspMI	CCCGGG	1 cut(s)	1679
TspRI	CASTG	5 cut(s)	947, 978, 1813, 2038, 2555
Tth111I	GACNNNGTC	4 cut(s)	656, 659, 739, 1426
Van91I	CCANNNNNTGG	1 cut(s)	1885
VneI	GTGCAC	1 cut(s)	667
XapI	RAATTY	3 cut(s)	310, 1123, 2177
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1261
XmaI	CCCGGG	1 cut(s)	1679
XmaJI	CCTAGG	1 cut(s)	497
XspI	CTAG	5 cut(s)	498, 1560, 1650, 1890, 2405
ZraI	GACGTC	2 cut(s)	151, 658
Zsp2I	ATGCAT	2 cut(s)	1549, 2280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.