Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Forward (+)

75971002 .. 75974527

Gene Structure 3526 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G043280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2721 bp

ATGGATTTTTCGTTGAAGAAGTCATTCAAATCCCATGGCTCGAGCAAACACATGAGAAAGATCTCAGCCGGAGCAGTAGAAGATAACAGCCACGAGGAGCTCCCGATTCTAATTGATCACCAGAATCAGGCAAGCATCGCCTCAGATTCGCCGGGCCACCACCGCCGAGAGGTCATCGTCAAGATCGACGAGGGCGATTCCTCCGCAACATCCATCAGAGAAAACGTCAGGGAAGTGGAGCATGGCGGGACGATTTGGCGGGAATCGAGTTTAGATTTTTGGACGGAGGACGAAGGGACCACTATCCCCGCCGATGGAAGCGCCGGTAGCGGGGGCTTTCATTTCGTGCAGAGGGGGAAGCAGACTGCGCCGGCGGCGGAGGAGGATCCGCCGTCGAAGCTGATAGGGCAGTACCTTAAGAAGCAGAGAGCCTCGGGCGGCGACGTGTCGTTGGACATGGATTTGGAGATGGACGAGCTACAAAAACCGCCGCTGGCGGAGCCTCCAAGGATCAGCGAGGCGTCGCGGGAGCTCAAGGTGTCGTTCCAATCGCCGGCAAGGTTGGAGATCCCGATCCCCGAGACGGGGAACGATTCTGTTCGGAGGCGAAACAAGGACTCCGTGGTTGACGACGAACGACGTCGTAGCAGTCGGTTGAGTAACGGCGGAGCCGACGTCATTAGGTGCTCGTCGAACAAATCGTTTCGGCGAGAGGCTTCGTTTCAGAACAACAATTTGTTGAGGCTAAAGACCAAGTCTAGATTGATAGACCCTCCTGAAAGCTGGGACGAGGTTCGGGTCCCGAAATCGGGCGGCGGCGGTGACGATGACGACGAGGACCCGTTTTTGGAAGAGGATCTGCCTGATGAGTACAAGAGGGTAAAATTAGATGCATTGACTCTGCTCCAATGGCTTAGTTTGGTTTTGATCATTGGTGCCTTGGTTTGCACGCTTTCTCTAAGAGTTTTGAGACAGAGGCATCTGTGGAAATTGAAGCTGTGGAAATGGGAGGTGCTGATTTTGGTTTCGATTTGCGGGAGGCTGGTTTCGGGTTGGGTGATAAAGATCATGGTGTTCTTCGTGGAAAGGAATTTCCTTCTGCGCAAGAGGGTGTTGTATTTTGTTTATGGGATCAGAAGGGCTGTGCAGAACTGTATTTGGCTTGGTTTTGTTTTGACGGCTTGGCATTTCATGCTAATCAGGAAGGTTGAGCAGGAAACCAATAGCAAAATACTAGAGTATGTGACGAAAGGTTTGCTTTGTCTCTTGATCGGGGTTTTGCTATGGTTGTTAAAGACCCTGGTTGTGAAGGTGCTTGCTTCCTCTTTCCACGTTAGATCCTACTTTGATCGGATTCAGGACGCGCTTTTTAATCAGTATGTGATCCAAACTCTCTCTGGGCGTCCCTTGATTGAGGTACAGAATGCGGAGGATGAAGAAGAGAGGCTTGCTGAGGAGGTTAGGAAGTTACAGAATGCTGGGGCAACTATGCCTCCTGACCTCAGATCAAATGCATTCCCTTCTGCGAGAATTGGGAGGGCGGTTGGAGTGGCTCTGGGAGAAGTGGGAGGTTCATTGCTGCAATTTTCAAGACCAACAATGTCCAAGAAGTCAGAGGAGGCTGCAATTACCATTGATCATTTGCATAGGCTCAATCCAAAAAATGTATCTGCTTGGAATATGAAGAGGCTAATGAACATTGTTAGAAAAGGGCATCTTACGACACTCGATGAGCAAATTCTGGATTCCATTGAGGCAAAGGCTGCAGCTAAAAAGATATTTCGCAATGTGGCACGGCCTGGCTCAAAGCACATCTACCTGGAGGATTTGTTGCGTTTCATGGGAGAAGAGGAGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCCGAGACTCGCAGAATCCGCAAAAATTCCTTGAAAAATTGGGTGGTCAACTGCTTTAGAGAACGGAGAGCACTTTCTTTTACACTGAATGACACCAAAACAGCGGTGAACAGACTTCGACACGTGGTGGATGTAATAGTTGGCATTGTTATTATAGTTATATGGCTTCTTGTGCTGGATATTGTCACCAGCGCCAATCTTGTTTATGCAACTTCTCAGCTTGTGGTCGTGGCATTTGTTTTTGGTAACACTTGCAGGACAGTATTTGAAGCAGTCATATTTTTATTTGTTATGCACCCATTTGACGTGGGAGATCGATGTGAAATTCAAGGAGTACAGATGGTTGTAGAAGAAATGAATATCTTGACTACAGTTTTTCTAAGGTATGACAACATAAAAATCGTGTACCCGAACAGTACACTTTCAACTATGCCCATCAATAACTTCTATCGTAGTCCGGACACAGGAGATGCTGTTGAGTTCTGTGTCCATATATCTACTCCACAAGATAAGATTGCTGTTATGAAACAGAGAATGATAAGTTACATTGAAAGTAAAAAGGAGCACTGGTATCCTCAAGTACAGATCGTATTGAAGGATGTAGAAGAATTAAATAAGATGAGGTATGCATTGTGGCTAACACATAAGATGAACTTCCAAGATGTGGGAGAAAAGTGGGAAAGAAGAGCCCTTTTACTTGAAGAGATGGTAAGGATCTTCCAGGAGCTGGACATTCAGTATCGTTTGCTGCCTATTGACATAAATGTCCGCACCATGCCTCCCACAACCACCACCGGTGTGCCTCCCTCAAATTTTTCAGCAACCACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

906

Amino Acids

103.22

Weight (kDa)

8.48

Isoelectric Point (pI)

48.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	722 - 778	6.5e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2651
AatII	GACGTC	2 cut(s)	643, 678
Acc16I	TGCGCA	1 cut(s)	1103
AccB1I	GGYRCC	1 cut(s)	935
AccB7I	CCANNNNNTGG	2 cut(s)	2551, 2614
AccII	CGCG	2 cut(s)	526, 1364
AccIII	TCCGGA	1 cut(s)	2344
AcsI	RAATTY	5 cut(s)	1090, 1737, 1912, 2211, 2698
AcvI	CACGTG	1 cut(s)	2011
AcyI	GRCGYC	4 cut(s)	521, 640, 675, 1402
AdeI	CACNNNGTG	1 cut(s)	2014
AfaI	GTAC	7 cut(s)	413, 872, 1419, 2223, 2294, 2305, 2469
AflII	CTTAAG	1 cut(s)	416
AflIII	ACRYGT	2 cut(s)	444, 2008
AgeI	ACCGGT	1 cut(s)	2681
AhdI	GACNNNNNGTC	1 cut(s)	1869
AjiI	CACGTC	2 cut(s)	445, 2194
AjnI	CCWGG	4 cut(s)	1299, 1798, 1818, 2607
AleI	CACNNNNGTG	1 cut(s)	2684
Alw21I	GWGCWC	5 cut(s)	102, 534, 689, 1960, 2454
Alw26I	GTCTC	5 cut(s)	575, 964, 1268, 1877, 1886
AlwNI	CAGNNNCTG	1 cut(s)	2614
Ama87I	CYCGRG	3 cut(s)	40, 433, 578
Aor13HI	TCCGGA	1 cut(s)	2344
AoxI	GGCC	3 cut(s)	154, 1796, 1884
ApeKI	GCWGC	5 cut(s)	1579, 1622, 1763, 1766, 2635
ApoI	RAATTY	5 cut(s)	1090, 1737, 1912, 2211, 2698
ArsI	GACNNNNNNTTYG	2 cut(s)	1237, 1269
AsiGI	ACCGGT	1 cut(s)	2681
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	5 cut(s)	323, 370, 1104, 1366, 2081
AspS9I	GGNCC	5 cut(s)	154, 297, 799, 838, 1884
AsuC2I	CCSGG	1 cut(s)	153
AsuHPI	GGTGA	5 cut(s)	110, 833, 1069, 2005, 2065
AvaI	CYCGRG	3 cut(s)	40, 433, 578
AvaII	GGWCC	3 cut(s)	297, 799, 838
BamHI	GGATCC	1 cut(s)	385
BanI	GGYRCC	1 cut(s)	935
BanII	GRGCYC	3 cut(s)	102, 534, 2578
BauI	CACGAG	1 cut(s)	92
BbrPI	CACGTG	1 cut(s)	2011
Bbv12I	GWGCWC	5 cut(s)	102, 534, 689, 1960, 2454
BbvCI	CCTCAGC	1 cut(s)	1452
BbvI	GCAGC	5 cut(s)	1566, 1609, 1750, 1778, 2622
BccI	CCATC	6 cut(s)	221, 308, 463, 2221, 2330, 2587
BceAI	ACGGC	4 cut(s)	376, 679, 1194, 1811
BcgI	CGANNNNNNTGC	2 cut(s)	1237, 1271
BciT130I	CCWGG	4 cut(s)	1301, 1800, 1820, 2609
BciVI	GTATCC	1 cut(s)	2469
BclI	TGATCA	3 cut(s)	115, 927, 1636
BcnI	CCSGG	1 cut(s)	153
BcoDI	GTCTC	5 cut(s)	575, 964, 1268, 1877, 1886
BfaI	CTAG	2 cut(s)	759, 1235
BfmI	CTRYAG	2 cut(s)	1764, 2256
BfoI	RGCGCY	2 cut(s)	324, 2082
BfrI	CTTAAG	1 cut(s)	416
BfuI	GTATCC	1 cut(s)	2469
BglII	AGATCT	1 cut(s)	60
Bme1390I	CCNGG	5 cut(s)	153, 1301, 1800, 1820, 2609
Bme18I	GGWCC	3 cut(s)	297, 799, 838
BmeRI	GACNNNNNGTC	1 cut(s)	1869
BmeT110I	CYCGRG	3 cut(s)	40, 433, 578
BmgBI	CACGTC	2 cut(s)	445, 2194
BmgT120I	GGNCC	5 cut(s)	154, 297, 799, 838, 1884
BmiI	GGNNCC	9 cut(s)	298, 387, 501, 670, 800, 801, 840, 937, 1885
BmrFI	CCNGG	5 cut(s)	153, 1301, 1800, 1820, 2609
BmsI	GCATC	5 cut(s)	144, 880, 988, 1723, 2347
BpmI	CTGGAG	1 cut(s)	1841
Bpu10I	CCTNAGC	1 cut(s)	1452
BpuEI	CTTGAG	2 cut(s)	518, 2448
BpuMI	CCSGG	1 cut(s)	153
Bsa29I	ATCGAT	1 cut(s)	2203
BsaAI	YACGTR	1 cut(s)	2011
BsaBI	GATNNNNATC	2 cut(s)	572, 1064
BsaHI	GRCGYC	4 cut(s)	521, 640, 675, 1402
BsaJI	CCNNGG	6 cut(s)	34, 432, 506, 621, 939, 1299
BsaWI	WCCGGW	2 cut(s)	2344, 2681
Bse118I	RCCGGY	4 cut(s)	323, 370, 553, 2681
Bse1I	ACTGG	1 cut(s)	2459
Bse3DI	GCAATG	2 cut(s)	1574, 1792
Bse8I	GATNNNNATC	2 cut(s)	572, 1064
BseAI	TCCGGA	1 cut(s)	2344
BseBI	CCWGG	4 cut(s)	1301, 1800, 1820, 2609
BseCI	ATCGAT	1 cut(s)	2203
BseDI	CCNNGG	6 cut(s)	34, 432, 506, 621, 939, 1299
BseGI	GGATG	4 cut(s)	209, 1438, 2023, 2491
BseJI	GATNNNNATC	2 cut(s)	572, 1064
BseMI	GCAATG	2 cut(s)	1574, 1792
BseMII	CTCAG	5 cut(s)	78, 156, 1443, 1516, 2117
BseNI	ACTGG	1 cut(s)	2459
BseRI	GAGGAG	5 cut(s)	110, 395, 1469, 1631, 1865
BseXI	GCAGC	5 cut(s)	1566, 1609, 1750, 1778, 2622
BseYI	CCCAGC	2 cut(s)	783, 1478
BsgI	GTGCAG	2 cut(s)	368, 1166
Bsh1236I	CGCG	2 cut(s)	526, 1364
BshFI	GGCC	3 cut(s)	156, 1798, 1886
BshNI	GGYRCC	1 cut(s)	935
BshTI	ACCGGT	1 cut(s)	2681
BshVI	ATCGAT	1 cut(s)	2203
BsiHKAI	GWGCWC	5 cut(s)	102, 534, 689, 1960, 2454
BsiHKCI	CYCGRG	3 cut(s)	40, 433, 578
BsiSI	CCGG	7 cut(s)	69, 152, 324, 371, 554, 2345, 2682
BslFI	GGGAC	5 cut(s)	262, 310, 785, 800, 1389
BsmAI	GTCTC	5 cut(s)	575, 964, 1268, 1877, 1886
BsmBI	CGTCTC	1 cut(s)	575
BsmFI	GGGAC	5 cut(s)	262, 310, 785, 800, 1389
BsmI	GAATGC	3 cut(s)	1429, 1480, 1514
BsnI	GGCC	3 cut(s)	156, 1798, 1886
BsoBI	CYCGRG	3 cut(s)	40, 433, 578
Bsp1286I	GDGCHC	6 cut(s)	102, 534, 689, 1960, 2454, 2578
Bsp13I	TCCGGA	1 cut(s)	2344
Bsp19I	CCATGG	1 cut(s)	34
BspANI	GGCC	3 cut(s)	156, 1798, 1886
BspCNI	CTCAG	5 cut(s)	77, 155, 1444, 1515, 2116
BspDI	ATCGAT	1 cut(s)	2203
BspEI	TCCGGA	1 cut(s)	2344
BspFNI	CGCG	2 cut(s)	526, 1364
BspLI	GGNNCC	9 cut(s)	298, 387, 501, 670, 800, 801, 840, 937, 1885
BspMAI	CTGCAG	1 cut(s)	1768
BspQI	GCTCTTC	1 cut(s)	2566
BspT107I	GGYRCC	1 cut(s)	935
BspTI	CTTAAG	1 cut(s)	416
BsrDI	GCAATG	2 cut(s)	1574, 1792
BsrFI	RCCGGY	4 cut(s)	323, 370, 553, 2681
BsrI	ACTGG	1 cut(s)	2459
BssAI	RCCGGY	4 cut(s)	323, 370, 553, 2681
BssECI	CCNNGG	6 cut(s)	34, 432, 506, 621, 939, 1299
BssNI	GRCGYC	4 cut(s)	521, 640, 675, 1402
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	3 cut(s)	34, 506, 939
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	4 cut(s)	1301, 1800, 1820, 2609
Bst4CI	ACNGT	4 cut(s)	1154, 2149, 2260, 2303
Bst6I	CTCTTC	6 cut(s)	846, 1434, 1679, 1842, 2566, 2583
BstACI	GRCGYC	4 cut(s)	521, 640, 675, 1402
BstAFI	CTTAAG	1 cut(s)	416
BstAPI	GCANNNNNTGC	2 cut(s)	1192, 1763
BstBAI	YACGTR	1 cut(s)	2011
BstC8I	GCNNGC	7 cut(s)	133, 372, 495, 555, 950, 1317, 1449
BstDEI	CTNAG	8 cut(s)	64, 142, 914, 959, 1452, 1502, 2103, 2267
BstDSI	CCRYGG	2 cut(s)	34, 621
BstF5I	GGATG	4 cut(s)	209, 1438, 2023, 2491
BstFNI	CGCG	2 cut(s)	526, 1364
BstH2I	RGCGCY	2 cut(s)	324, 2082
BstHHI	GCGC	5 cut(s)	323, 370, 1104, 1366, 2081
BstMAI	GTCTC	5 cut(s)	575, 964, 1268, 1877, 1886
BstNI	CCWGG	4 cut(s)	1301, 1800, 1820, 2609
BstSCI	CCNGG	5 cut(s)	151, 1299, 1798, 1818, 2607
BstSFI	CTRYAG	2 cut(s)	1764, 2256
BstUI	CGCG	2 cut(s)	526, 1364
BstV1I	GCAGC	5 cut(s)	1566, 1609, 1750, 1778, 2622
BstX2I	RGATCY	6 cut(s)	60, 385, 567, 856, 1337, 2601
BstYI	RGATCY	6 cut(s)	60, 385, 567, 856, 1337, 2601
Bsu15I	ATCGAT	1 cut(s)	2203
BsuI	GTATCC	1 cut(s)	2469
BsuRI	GGCC	3 cut(s)	156, 1798, 1886
BsuTUI	ATCGAT	1 cut(s)	2203
BtgI	CCRYGG	2 cut(s)	34, 621
BtgZI	GCGATG	1 cut(s)	121
BtrI	CACGTC	2 cut(s)	445, 2194
BtsCI	GGATG	4 cut(s)	209, 1438, 2023, 2491
BtsIMutI	CAGTG	2 cut(s)	1970, 2452
Cac8I	GCNNGC	7 cut(s)	133, 372, 495, 555, 950, 1317, 1449
CaiI	CAGNNNCTG	1 cut(s)	2614
CfoI	GCGC	5 cut(s)	323, 370, 1104, 1366, 2081
Cfr10I	RCCGGY	4 cut(s)	323, 370, 553, 2681
Cfr13I	GGNCC	5 cut(s)	154, 297, 799, 838, 1884
ClaI	ATCGAT	1 cut(s)	2203
CseI	GACGC	3 cut(s)	510, 1370, 1391
Csp6I	GTAC	7 cut(s)	412, 871, 1418, 2222, 2293, 2304, 2468
CspAI	ACCGGT	1 cut(s)	2681
CviAII	CATG	9 cut(s)	35, 52, 242, 457, 1069, 1192, 1840, 1867, 2662
CviQI	GTAC	7 cut(s)	412, 871, 1418, 2222, 2293, 2304, 2468
DdeI	CTNAG	8 cut(s)	64, 142, 914, 959, 1452, 1502, 2103, 2267
DraI	TTTAAA	1 cut(s)	1860
DraIII	CACNNNGTG	1 cut(s)	2014
DrdI	GACNNNNNNGTC	1 cut(s)	2651
DriI	GACNNNNNGTC	1 cut(s)	1869
DseDI	GACNNNNNNGTC	1 cut(s)	2651
Eam1104I	CTCTTC	6 cut(s)	846, 1434, 1679, 1842, 2566, 2583
Eam1105I	GACNNNNNGTC	1 cut(s)	1869
EarI	CTCTTC	6 cut(s)	846, 1434, 1679, 1842, 2566, 2583
EciI	GGCGGA	4 cut(s)	378, 392, 512, 681
Ecl136II	GAGCTC	2 cut(s)	100, 532
Eco130I	CCWWGG	3 cut(s)	34, 506, 939
Eco24I	GRGCYC	3 cut(s)	102, 534, 2578
Eco47I	GGWCC	3 cut(s)	297, 799, 838
Eco53kI	GAGCTC	2 cut(s)	100, 532
Eco72I	CACGTG	1 cut(s)	2011
Eco88I	CYCGRG	3 cut(s)	40, 433, 578
EcoICRI	GAGCTC	2 cut(s)	100, 532
EcoO109I	RGGNCCY	3 cut(s)	799, 838, 1884
EcoRII	CCWGG	4 cut(s)	1299, 1798, 1818, 2607
EcoT14I	CCWWGG	3 cut(s)	34, 506, 939
EcoT22I	ATGCAT	3 cut(s)	895, 1516, 2518
EcoT38I	GRGCYC	3 cut(s)	102, 534, 2578
ErhI	CCWWGG	3 cut(s)	34, 506, 939
Esp3I	CGTCTC	1 cut(s)	575
FaeI	CATG	9 cut(s)	38, 55, 245, 460, 1072, 1195, 1843, 1870, 2665
FalI	AAGNNNNNCTT	8 cut(s)	1242, 1274, 1431, 1463, 1701, 1733, 2525, 2557
FaqI	GGGAC	5 cut(s)	262, 310, 785, 800, 1389
FatI	CATG	9 cut(s)	34, 51, 241, 456, 1068, 1191, 1839, 1866, 2661
FauI	CCCGC	6 cut(s)	239, 252, 316, 323, 519, 1028
FbaI	TGATCA	3 cut(s)	115, 927, 1636
FokI	GGATG	4 cut(s)	196, 1445, 2030, 2498
FriOI	GRGCYC	3 cut(s)	102, 534, 2578
FspBI	CTAG	2 cut(s)	759, 1235
FspI	TGCGCA	1 cut(s)	1103
GlaI	GCGC	5 cut(s)	322, 369, 1103, 1365, 2080
GsaI	CCCAGC	2 cut(s)	787, 1482
GsuI	CTGGAG	1 cut(s)	1841
HaeII	RGCGCY	2 cut(s)	324, 2082
HaeIII	GGCC	3 cut(s)	156, 1798, 1886
HapII	CCGG	7 cut(s)	69, 152, 324, 371, 554, 2345, 2682
HgaI	GACGC	3 cut(s)	510, 1370, 1391
HhaI	GCGC	5 cut(s)	323, 370, 1104, 1366, 2081
Hin1I	GRCGYC	4 cut(s)	521, 640, 675, 1402
Hin1II	CATG	9 cut(s)	38, 55, 245, 460, 1072, 1195, 1843, 1870, 2665
Hin6I	GCGC	5 cut(s)	321, 368, 1102, 1364, 2079
HinP1I	GCGC	5 cut(s)	321, 368, 1102, 1364, 2079
HincII	GTYRAC	2 cut(s)	628, 1936
HindII	GTYRAC	2 cut(s)	628, 1936
HpaII	CCGG	7 cut(s)	69, 152, 324, 371, 554, 2345, 2682
HphI	GGTGA	5 cut(s)	110, 833, 1069, 2005, 2065
Hpy166II	GTNNAC	5 cut(s)	628, 1936, 1996, 2293, 2306
Hpy188I	TCNGA	9 cut(s)	145, 218, 603, 726, 1136, 1353, 1505, 1615, 1891
Hpy8I	GTNNAC	5 cut(s)	628, 1936, 1996, 2293, 2306
HpyAV	CCTTC	8 cut(s)	287, 1106, 1131, 1198, 1303, 1530, 1874, 2476
HpyCH4III	ACNGT	4 cut(s)	1154, 2149, 2260, 2303
HpyCH4IV	ACGT	7 cut(s)	225, 444, 640, 675, 1332, 2010, 2193
HpyF3I	CTNAG	8 cut(s)	64, 142, 914, 959, 1452, 1502, 2103, 2267
HpySE526I	ACGT	7 cut(s)	225, 444, 640, 675, 1332, 2010, 2193
Hsp92I	GRCGYC	4 cut(s)	521, 640, 675, 1402
Hsp92II	CATG	9 cut(s)	38, 55, 245, 460, 1072, 1195, 1843, 1870, 2665
HspAI	GCGC	5 cut(s)	321, 368, 1102, 1364, 2079
KflI	GGGWCCC	1 cut(s)	799
Kpn2I	TCCGGA	1 cut(s)	2344
KroI	GCCGGC	2 cut(s)	370, 553
KroNI	GCCGGC	2 cut(s)	372, 555
Ksp22I	TGATCA	3 cut(s)	115, 927, 1636
LguI	GCTCTTC	1 cut(s)	2566
Lsp1109I	GCAGC	5 cut(s)	1566, 1609, 1750, 1778, 2622
LweI	GCATC	5 cut(s)	144, 880, 988, 1723, 2347
MaeI	CTAG	2 cut(s)	759, 1235
MaeII	ACGT	7 cut(s)	225, 444, 640, 675, 1332, 2010, 2193
MaeIII	GTNAC	7 cut(s)	659, 821, 1243, 1467, 2071, 2132, 2429
MflI	RGATCY	6 cut(s)	60, 385, 567, 856, 1337, 2601
MhlI	GDGCHC	6 cut(s)	102, 534, 689, 1960, 2454, 2578
MlyI	GAGTC	4 cut(s)	611, 892, 1879, 1888
MmeI	TCCRAC	3 cut(s)	432, 543, 1525
Mph1103I	ATGCAT	3 cut(s)	895, 1516, 2518
MreI	CGCCGGCG	1 cut(s)	370
MroI	TCCGGA	1 cut(s)	2344
MroNI	GCCGGC	2 cut(s)	370, 553
MroXI	GAANNNNTTC	1 cut(s)	23
MseI	TTAA	5 cut(s)	417, 1292, 1371, 1859, 2498
MslI	CAYNNNNRTG	1 cut(s)	2684
MspA1I	CMGCKG	2 cut(s)	493, 1991
MspCI	CTTAAG	1 cut(s)	416
MspI	CCGG	7 cut(s)	69, 152, 324, 371, 554, 2345, 2682
MspR9I	CCNGG	5 cut(s)	153, 1301, 1800, 1820, 2609
Mva1269I	GAATGC	3 cut(s)	1429, 1480, 1514
MvaI	CCWGG	4 cut(s)	1301, 1800, 1820, 2609
MvnI	CGCG	2 cut(s)	526, 1364
NaeI	GCCGGC	2 cut(s)	372, 555
NciI	CCSGG	1 cut(s)	153
NcoI	CCATGG	1 cut(s)	34
NgoMIV	GCCGGC	2 cut(s)	370, 553
NlaIII	CATG	9 cut(s)	38, 55, 245, 460, 1072, 1195, 1843, 1870, 2665
NlaIV	GGNNCC	9 cut(s)	298, 387, 501, 670, 800, 801, 840, 937, 1885
NmeAIII	GCCGAG	1 cut(s)	191
NmuCI	GTSAC	3 cut(s)	821, 1243, 2071
NsbI	TGCGCA	1 cut(s)	1103
NsiI	ATGCAT	3 cut(s)	895, 1516, 2518
OliI	CACNNNNGTG	1 cut(s)	2684
PaeR7I	CTCGAG	1 cut(s)	40
PciSI	GCTCTTC	1 cut(s)	2566
PcsI	WCGNNNNNNNCGW	5 cut(s)	183, 192, 669, 698, 831
PctI	GAATGC	3 cut(s)	1429, 1480, 1514
PdiI	GCCGGC	2 cut(s)	372, 555
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	9 cut(s)	106, 124, 146, 197, 263, 593, 1354, 1745, 1902
PflFI	GACNNNGTC	1 cut(s)	754
PflMI	CCANNNNNTGG	2 cut(s)	2551, 2614
PfoI	TCCNGGA	1 cut(s)	2607
PinAI	ACCGGT	1 cut(s)	2681
PleI	GAGTC	4 cut(s)	611, 892, 1878, 1888
PmaCI	CACGTG	1 cut(s)	2011
PmlI	CACGTG	1 cut(s)	2011
PpsI	GAGTC	4 cut(s)	611, 892, 1878, 1888
Ppu21I	YACGTR	1 cut(s)	2011
PpuMI	RGGWCCY	2 cut(s)	799, 838
Psp124BI	GAGCTC	2 cut(s)	102, 534
Psp5II	RGGWCCY	2 cut(s)	799, 838
Psp6I	CCWGG	4 cut(s)	1299, 1798, 1818, 2607
PspCI	CACGTG	1 cut(s)	2011
PspFI	CCCAGC	2 cut(s)	783, 1478
PspGI	CCWGG	4 cut(s)	1299, 1798, 1818, 2607
PspN4I	GGNNCC	9 cut(s)	298, 387, 501, 670, 800, 801, 840, 937, 1885
PspPI	GGNCC	5 cut(s)	154, 297, 799, 838, 1884
PspPPI	RGGWCCY	2 cut(s)	799, 838
PspXI	VCTCGAGB	1 cut(s)	40
PstI	CTGCAG	1 cut(s)	1768
PstNI	CAGNNNCTG	1 cut(s)	2614
PsuI	RGATCY	6 cut(s)	60, 385, 567, 856, 1337, 2601
PsyI	GACNNNGTC	1 cut(s)	754
RsaI	GTAC	7 cut(s)	413, 872, 1419, 2223, 2294, 2305, 2469
RsaNI	GTAC	7 cut(s)	412, 871, 1418, 2222, 2293, 2304, 2468
RseI	CAYNNNNRTG	1 cut(s)	2684
SacI	GAGCTC	2 cut(s)	102, 534
SapI	GCTCTTC	1 cut(s)	2566
SaqAI	TTAA	5 cut(s)	417, 1292, 1371, 1859, 2498
Sau96I	GGNCC	5 cut(s)	154, 297, 799, 838, 1884
SchI	GAGTC	4 cut(s)	611, 892, 1879, 1888
ScrFI	CCNGG	5 cut(s)	153, 1301, 1800, 1820, 2609
SduI	GDGCHC	6 cut(s)	102, 534, 689, 1960, 2454, 2578
SfaNI	GCATC	5 cut(s)	144, 880, 988, 1723, 2347
SfcI	CTRYAG	2 cut(s)	1764, 2256
Sfr274I	CTCGAG	1 cut(s)	40
SgrAI	CRCCGGYG	2 cut(s)	370, 2681
SinI	GGWCC	3 cut(s)	297, 799, 838
SlaI	CTCGAG	1 cut(s)	40
SmiMI	CAYNNNNRTG	1 cut(s)	2684
SmlI	CTYRAG	4 cut(s)	40, 416, 533, 2463
SmoI	CTYRAG	4 cut(s)	40, 416, 533, 2463
SspMI	CTAG	2 cut(s)	759, 1235
SstI	GAGCTC	2 cut(s)	102, 534
StyD4I	CCNGG	5 cut(s)	151, 1299, 1798, 1818, 2607
StyI	CCWWGG	3 cut(s)	34, 506, 939
TaaI	ACNGT	4 cut(s)	1154, 2149, 2260, 2303
TaiI	ACGT	7 cut(s)	228, 447, 643, 678, 1335, 2013, 2196
TaqI	TCGA	9 cut(s)	41, 186, 266, 395, 692, 1028, 1728, 2005, 2203
TatI	WGTACW	4 cut(s)	870, 2221, 2303, 2467
TauI	GCSGC	5 cut(s)	377, 441, 493, 816, 819
TfiI	GAWTC	9 cut(s)	106, 124, 146, 197, 263, 593, 1354, 1745, 1902
Tru1I	TTAA	5 cut(s)	417, 1292, 1371, 1859, 2498
Tru9I	TTAA	5 cut(s)	417, 1292, 1371, 1859, 2498
TscAI	CASTG	2 cut(s)	1977, 2459
TseFI	GTSAC	3 cut(s)	821, 1243, 2071
TseI	GCWGC	5 cut(s)	1579, 1622, 1763, 1766, 2635
Tsp45I	GTSAC	3 cut(s)	821, 1243, 2071
TspGWI	ACGGA	3 cut(s)	299, 610, 1966
TspRI	CASTG	2 cut(s)	1977, 2459
Tth111I	GACNNNGTC	1 cut(s)	754
Van91I	CCANNNNNTGG	2 cut(s)	2551, 2614
Vha464I	CTTAAG	1 cut(s)	416
VpaK11BI	GGWCC	3 cut(s)	297, 799, 838
XapI	RAATTY	5 cut(s)	1090, 1737, 1912, 2211, 2698
XbaI	TCTAGA	1 cut(s)	758
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1394
XhoI	CTCGAG	1 cut(s)	40
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	2 cut(s)	759, 1235
ZraI	GACGTC	2 cut(s)	641, 676
Zsp2I	ATGCAT	3 cut(s)	895, 1516, 2518

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.