pycom08g00580

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0001376
Physical Location & Seq
Reverse (-)
476532 .. 479310
2779 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g00580.2

Sequence Viewer

Length: 2301 bp
ATGGCAACGAGGGAAGGAGAAGTGGTAGTCAAGATCGACGATGGTGATGGTTCCTGTAGCATAGAAACAGAGACTGCACCGTATAACGGAGAACCAGCTCCAAGGACACCAGTAATTCAGGGCTCCCCAGAAAAACTGACTGAATCTTTCCGGCGGAGAAGCAAAGAGCTTCCGGAAGCTTCCTCTAGCTCCGACGGGGCTGAGGCTGTCATCCAATGCAGCTCCAACGCCTCCTTCTCCCGAAGCCCCTGGAAACCCCCCATGAGCAAGACAAAGTCCAGGCTTCTCGACCTACCAGAGGAAGTGTCTCTGAAATCCGACAGAACGGCCGCTTCCGGACGATCGCATGGAAAAGATGACGACGCTTCGGCTTTCGACGACCCCGACCTTGAAGACATTCCAGAGGAGTACAGGAAGTGGAAGTTCAGCGCGCTCACGTTGCTTCAGTGGGTGAGTCTTGTTATTGTTATAGCTGCTTTGGTTTGCAATCTTTGGATTCCGATTTTAAAGAAGCAAACTTTGTGGGATCTTCCGCTGTGGAAGTGGGAAATTCTGGTTCTGGCATTGATTTGTGGTCGTTTGGTTTCTGGTTGGGGAATTCGGGTGGTTGTGTTCTTAGTAGAACGAAATTTCTTGTTGCGAAAACGGGTTTTGTATTTCGTTTATGGATTAAGGAAATCGGTGCAGAATTGTCTCTGGTTGGGTTTGGTCTTGTTGGCGTGGCACTTGATATTTGATAAGAAAGTGCAGGAGAAGACCAAGAGTAGGATTTTGCCTTATGTGACCAAGATTCTTGTGTGTTTTCTGGTTGGGACATTGATTTGGCTGCTGAAGACAATACTTGTTAAGGTTCTTGCCTTGTCTTTCCATGTCAACGCATTTTTCGATCGAATTCAGGAGGCTTTGTTCAATCAGTATGTGTTGGAGACACTTTCAGGCCCCCCTCTGTTTGAAAGGCGACTGTCACTAGAAGAGGAAAAAGCCGCGGCAGCTGAGATTCAGGAGTTTCAGAAAGCGGGGGCTGCTATGCCTCGCGAGCTCAGTGCAACTCTTCTTCGCAGGGCAAGGAGTGGGAGAGTTGTGGGGAGTGGACACCAAAATAGCCCCAGAGTAGGGAAGAGTCCCGCGGTTTCTAGGCCAGCGTCTGGGAAGCAAGATGAAGGCATCCCGGTTGATCACTTGCATAAGCTAAACCAGAAGAATGTGTCGGCTTGGAATATGAGAAAAATGGTAAACATCATCCGGCACGGGGCCTTAACCACTCTAGATGAACACATACTGAATTCTGATATGGGAGACAACTCTTCTTTGATGATTACAACTGAATCTCAAGCAAAAGATGCAGCCAGGAAGATGTTTTTCAGAGTAGCCAAGCCAGGATACATTTACCTCGAGGATTTGATGCAGTTTATGGATAAAGATGAAGCTTTAAAGACATTTCGTCTCTTTGGTGCAGCAAGTGAAGATGACAAAATCAGCAAGACCGCTCTTAGGAATTGGGTGGTCAATGCATTTAAAGACAGAAGAGCACTAGCATTTTCTTTGAACGATACAAAAACAGCTGTGGATGAACTCCATAACATTTTAAACGTCCTTGTAGCTATCGTCATTTTGATAATCTGGCTTATTATACTTGAGGTTCATTTCGCCCACTTCCTTCTCTTAATGAGCTCCCAGCTCCTTTTGGTTGTATTTATCTTTGGGAACACATGCAAGACAGTTTTTGAAGCAATCATCTTTCTATTTGTAATGCACCCGTTTGATGTGGGTGATCGTTGTGAAGTGGATGGAGTCCAGATGGTAGTTGAAGAGATGAATATATTAACTACGGTCTTTCTGAAGTTTGATAACCACAAGGTCATATACCCTAACAGTGTTCTTGCTACCAAGGCAATTGCTAACTATCGTCGTAGTCCTGACATGGGCGATGCTATTGATTTTTGTGTGCATATTTCAACTCCTTTGGAGAAGGTGGCTACTATGAAAGGAAGAATACAAAGGTATATTGAAGACAAGAAGGACCACTGGTACGACGCTCCAATGTTGATCATGAGGGATGTGGAGGAGTTGAACAAACTGAAGATATCGGTATGGCTGACACACAAGATGAACCATCAGGACATGCTGGAGAGATGGCACAGGAGGAGCCTTTTGATCGAAGAGATGATCAAAGTGTTCAGAGAGCTAGATATTGAGTACCGGTTACTGCCTCTCGATGTCAACATCCGAGCCATGCCAAGTTCAACCTCCAACAAGCTTCCTTCAAATTGGACAACTTGTGTCCCGCCACCAGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0016020 GO:0044464 GO:0051179 GO:0051234 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel

Protein Analysis

767

Amino Acids

87.19

Weight (kDa)

8.58

Isoelectric Point (pI)

50.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1391
AccB7I CCANNNNNTGG 1 cut(s) 1145
AccBSI CCGCTC 1 cut(s) 1487
AccII CGCG 4 cut(s) 431, 986, 1035, 1127
AccIII TCCGGA 2 cut(s) 172, 335
AclWI GGATC 1 cut(s) 534
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 5 cut(s) 549, 597, 628, 891, 1282
AcuI CTGAAG 4 cut(s) 428, 851, 1859, 2099
AfaI GTAC 3 cut(s) 410, 2030, 2198
AfiI CCNNNNNNNGG 9 cut(s) 86, 298, 765, 1112, 1113, 1145, 1249, 1491, 1922
AgeI ACCGGT 1 cut(s) 2199
AhdI GACNNNNNGTC 1 cut(s) 1440
AjnI CCWGG 4 cut(s) 248, 278, 1346, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AloI GAACNNNNNNTCC 2 cut(s) 890, 922
Alw21I GWGCWC 3 cut(s) 1041, 1531, 1673
Alw26I GTCTC 6 cut(s) 65, 312, 698, 920, 1290, 1448
AlwI GGATC 1 cut(s) 534
AlwNI CAGNNNCTG 2 cut(s) 74, 1145
Ama87I CYCGRG 1 cut(s) 1391
Aor13HI TCCGGA 2 cut(s) 172, 335
AoxI GGCC 4 cut(s) 327, 937, 1136, 1251
ApeKI GCWGC 7 cut(s) 219, 473, 826, 989, 1022, 1343, 1454
ApoI RAATTY 5 cut(s) 549, 597, 628, 891, 1282
AsiGI ACCGGT 1 cut(s) 2199
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 2 cut(s) 431, 433
AspS9I GGNCC 3 cut(s) 938, 1251, 2020
AsuC2I CCSGG 1 cut(s) 1169
AsuHPI GGTGA 3 cut(s) 56, 463, 1781
AvaI CYCGRG 1 cut(s) 1391
AvaII GGWCC 1 cut(s) 2020
BanII GRGCYC 3 cut(s) 125, 1041, 1673
BbsI GAAGAC 4 cut(s) 399, 761, 839, 2016
Bbv12I GWGCWC 3 cut(s) 1041, 1531, 1673
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1466
BccI CCATC 6 cut(s) 35, 41, 1781, 1792, 2121, 2127
BceAI ACGGC 1 cut(s) 342
BcgI CGANNNNNNTGC 2 cut(s) 1025, 1059
BciT130I CCWGG 4 cut(s) 250, 280, 1348, 1377
BciVI GTATCC 1 cut(s) 1373
BclI TGATCA 3 cut(s) 1174, 2046, 2166
BcnI CCSGG 1 cut(s) 1169
BcoDI GTCTC 6 cut(s) 65, 312, 698, 920, 1290, 1448
BfaI CTAG 6 cut(s) 186, 968, 1134, 1265, 1532, 2186
BfmI CTRYAG 1 cut(s) 55
BfuI GTATCC 1 cut(s) 1373
Bme1390I CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
Bme18I GGWCC 1 cut(s) 2020
BmeRI GACNNNNNGTC 1 cut(s) 1440
BmeT110I CYCGRG 1 cut(s) 1391
BmgT120I GGNCC 3 cut(s) 938, 1251, 2020
BmiI GGNNCC 5 cut(s) 52, 124, 940, 1252, 2147
BmrFI CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
BmsI GCATC 4 cut(s) 1173, 1330, 1392, 1918
BpiI GAAGAC 4 cut(s) 399, 761, 839, 2016
BpmI CTGGAG 1 cut(s) 2147
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 1314, 1655
BpuMI CCSGG 1 cut(s) 1169
BsaJI CCNNGG 5 cut(s) 101, 248, 984, 1125, 1887
BsaWI WCCGGW 3 cut(s) 172, 335, 2199
BsaXI ACNNNNNCTCC 4 cut(s) 9, 39, 890, 920
Bsc4I CCNNNNNNNGG 9 cut(s) 86, 298, 765, 1112, 1113, 1145, 1249, 1491, 1922
Bse118I RCCGGY 1 cut(s) 2199
Bse1I ACTGG 2 cut(s) 110, 2030
BseAI TCCGGA 2 cut(s) 172, 335
BseBI CCWGG 4 cut(s) 250, 280, 1348, 1377
BseDI CCNNGG 5 cut(s) 101, 248, 984, 1125, 1887
BseGI GGATG 7 cut(s) 210, 1164, 1239, 1573, 1792, 2062, 2223
BseLI CCNNNNNNNGG 9 cut(s) 86, 298, 765, 1112, 1113, 1145, 1249, 1491, 1922
BseMII CTCAG 3 cut(s) 192, 984, 1054
BseNI ACTGG 2 cut(s) 110, 2030
BsePI GCGCGC 1 cut(s) 429
BseRI GAGGAG 3 cut(s) 419, 2078, 2158
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1466
BseYI CCCAGC 1 cut(s) 1674
BsgI GTGCAG 4 cut(s) 60, 704, 767, 1473
Bsh1236I CGCG 4 cut(s) 431, 986, 1035, 1127
Bsh1285I CGRYCG 3 cut(s) 330, 344, 889
BshFI GGCC 4 cut(s) 329, 939, 1138, 1253
BshTI ACCGGT 1 cut(s) 2199
BsiEI CGRYCG 3 cut(s) 330, 344, 889
BsiHKAI GWGCWC 3 cut(s) 1041, 1531, 1673
BsiHKCI CYCGRG 1 cut(s) 1391
BsiSI CCGG 6 cut(s) 151, 173, 336, 1169, 1243, 2200
BslFI GGGAC 3 cut(s) 826, 1107, 2267
BslI CCNNNNNNNGG 9 cut(s) 86, 298, 765, 1112, 1113, 1145, 1249, 1491, 1922
BsmAI GTCTC 6 cut(s) 65, 312, 698, 920, 1290, 1448
BsmBI CGTCTC 1 cut(s) 1448
BsmFI GGGAC 3 cut(s) 826, 1107, 2267
BsnI GGCC 4 cut(s) 329, 939, 1138, 1253
BsoBI CYCGRG 1 cut(s) 1391
Bsp1286I GDGCHC 4 cut(s) 125, 1041, 1531, 1673
Bsp13I TCCGGA 2 cut(s) 172, 335
Bsp143I GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
Bsp68I TCGCGA 1 cut(s) 1035
BspANI GGCC 4 cut(s) 329, 939, 1138, 1253
BspCNI CTCAG 3 cut(s) 193, 985, 1053
BspEI TCCGGA 2 cut(s) 172, 335
BspFNI CGCG 4 cut(s) 431, 986, 1035, 1127
BspHI TCATGA 1 cut(s) 2049
BspLI GGNNCC 5 cut(s) 52, 124, 940, 1252, 2147
BspPI GGATC 1 cut(s) 534
BspQI GCTCTTC 1 cut(s) 1519
BsrBI CCGCTC 1 cut(s) 1487
BsrFI RCCGGY 1 cut(s) 2199
BsrI ACTGG 2 cut(s) 110, 2030
BssAI RCCGGY 1 cut(s) 2199
BssECI CCNNGG 5 cut(s) 101, 248, 984, 1125, 1887
BssHII GCGCGC 1 cut(s) 429
BssMI GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
BssT1I CCWWGG 2 cut(s) 101, 1887
Bst2UI CCWGG 4 cut(s) 250, 280, 1348, 1377
Bst4CI ACNGT 5 cut(s) 81, 963, 1720, 1831, 1874
Bst6I CTCTTC 7 cut(s) 966, 1056, 1112, 1309, 1519, 1803, 2154
BstAPI GCANNNNNTGC 1 cut(s) 1340
BstC8I GCNNGC 3 cut(s) 431, 1037, 1140
BstDEI CTNAG 5 cut(s) 201, 616, 993, 1040, 1490
BstDSI CCRYGG 2 cut(s) 984, 1125
BstENI CCTNNNNNAGG 1 cut(s) 296
BstF5I GGATG 7 cut(s) 210, 1164, 1239, 1573, 1792, 2062, 2223
BstFNI CGCG 4 cut(s) 431, 986, 1035, 1127
BstHHI GCGC 2 cut(s) 431, 433
BstKTI GATC 9 cut(s) 36, 344, 529, 889, 1177, 1774, 2049, 2157, 2169
BstMAI GTCTC 6 cut(s) 65, 312, 698, 920, 1290, 1448
BstMBI GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
BstMCI CGRYCG 3 cut(s) 330, 344, 889
BstMWI GCNNNNNNNGC 6 cut(s) 439, 989, 1022, 1036, 1340, 1889
BstNI CCWGG 4 cut(s) 250, 280, 1348, 1377
BstNSI RCATGY 2 cut(s) 1713, 2125
BstSCI CCNGG 5 cut(s) 248, 278, 1167, 1346, 1375
BstSFI CTRYAG 1 cut(s) 55
BstUI CGCG 4 cut(s) 431, 986, 1035, 1127
BstV1I GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1466
BstV2I GAAGAC 4 cut(s) 399, 761, 839, 2016
BstX2I RGATCY 1 cut(s) 526
BstYI RGATCY 1 cut(s) 526
BstZI CGGCCG 1 cut(s) 327
BsuI GTATCC 1 cut(s) 1373
BsuRI GGCC 4 cut(s) 329, 939, 1138, 1253
BtgI CCRYGG 2 cut(s) 984, 1125
BtgZI GCGATG 1 cut(s) 1941
BtsCI GGATG 7 cut(s) 210, 1164, 1239, 1573, 1792, 2062, 2223
BtsIMutI CAGTG 4 cut(s) 452, 1048, 1879, 2023
BtuMI TCGCGA 1 cut(s) 1035
Cac8I GCNNGC 3 cut(s) 431, 1037, 1140
CaiI CAGNNNCTG 2 cut(s) 74, 1145
CciI TCATGA 1 cut(s) 2049
CfoI GCGC 2 cut(s) 431, 433
Cfr10I RCCGGY 1 cut(s) 2199
Cfr13I GGNCC 3 cut(s) 938, 1251, 2020
Cfr42I CCGCGG 2 cut(s) 987, 1128
CseI GACGC 3 cut(s) 371, 1131, 2042
Csp6I GTAC 3 cut(s) 409, 2029, 2197
CspAI ACCGGT 1 cut(s) 2199
CspCI CAANNNNNGTGG 2 cut(s) 503, 538
CviAII CATG 8 cut(s) 262, 347, 869, 1710, 1921, 2050, 2122, 2233
CviQI GTAC 3 cut(s) 409, 2029, 2197
DdeI CTNAG 5 cut(s) 201, 616, 993, 1040, 1490
DpnI GATC 9 cut(s) 35, 343, 528, 888, 1176, 1773, 2048, 2156, 2168
DpnII GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
DraI TTTAAA 4 cut(s) 507, 1431, 1516, 1587
DriI GACNNNNNGTC 1 cut(s) 1440
EaeI YGGCCR 1 cut(s) 327
EagI CGGCCG 1 cut(s) 327
Eam1104I CTCTTC 7 cut(s) 966, 1056, 1112, 1309, 1519, 1803, 2154
Eam1105I GACNNNNNGTC 1 cut(s) 1440
EarI CTCTTC 7 cut(s) 966, 1056, 1112, 1309, 1519, 1803, 2154
EciI GGCGGA 1 cut(s) 169
Ecl136II GAGCTC 2 cut(s) 1039, 1671
EclXI CGGCCG 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 101, 1887
Eco24I GRGCYC 3 cut(s) 125, 1041, 1673
Eco32I GATATC 1 cut(s) 2085
Eco47I GGWCC 1 cut(s) 2020
Eco52I CGGCCG 1 cut(s) 327
Eco53kI GAGCTC 2 cut(s) 1039, 1671
Eco57I CTGAAG 4 cut(s) 428, 851, 1859, 2099
Eco88I CYCGRG 1 cut(s) 1391
EcoICRI GAGCTC 2 cut(s) 1039, 1671
EcoNI CCTNNNNNAGG 1 cut(s) 296
EcoO109I RGGNCCY 2 cut(s) 938, 1251
EcoRI GAATTC 3 cut(s) 597, 891, 1282
EcoRII CCWGG 4 cut(s) 248, 278, 1346, 1375
EcoRV GATATC 1 cut(s) 2085
EcoT14I CCWWGG 2 cut(s) 101, 1887
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 3 cut(s) 125, 1041, 1673
ErhI CCWWGG 2 cut(s) 101, 1887
Esp3I CGTCTC 1 cut(s) 1448
FaeI CATG 8 cut(s) 265, 350, 872, 1713, 1924, 2053, 2125, 2236
FaqI GGGAC 3 cut(s) 826, 1107, 2267
FatI CATG 8 cut(s) 261, 346, 868, 1709, 1920, 2049, 2121, 2232
FauI CCCGC 3 cut(s) 1009, 1132, 2292
FbaI TGATCA 3 cut(s) 1174, 2046, 2166
FokI GGATG 7 cut(s) 197, 1151, 1226, 1580, 1799, 2069, 2210
FriOI GRGCYC 3 cut(s) 125, 1041, 1673
FspBI CTAG 6 cut(s) 186, 968, 1134, 1265, 1532, 2186
GlaI GCGC 2 cut(s) 430, 432
GsaI CCCAGC 1 cut(s) 1678
GsuI CTGGAG 1 cut(s) 2147
HaeIII GGCC 4 cut(s) 329, 939, 1138, 1253
HapII CCGG 6 cut(s) 151, 173, 336, 1169, 1243, 2200
HgaI GACGC 3 cut(s) 371, 1131, 2042
HhaI GCGC 2 cut(s) 431, 433
Hin1II CATG 8 cut(s) 265, 350, 872, 1713, 1924, 2053, 2125, 2236
Hin6I GCGC 2 cut(s) 429, 431
HinP1I GCGC 2 cut(s) 429, 431
HincII GTYRAC 2 cut(s) 874, 2221
HindII GTYRAC 2 cut(s) 874, 2221
HindIII AAGCTT 3 cut(s) 177, 1425, 2255
HinfI GANTC 8 cut(s) 143, 454, 496, 790, 997, 1120, 1325, 1791
HpaII CCGG 6 cut(s) 151, 173, 336, 1169, 1243, 2200
HphI GGTGA 3 cut(s) 56, 463, 1781
Hpy166II GTNNAC 4 cut(s) 874, 1091, 1234, 2221
Hpy8I GTNNAC 4 cut(s) 874, 1091, 1234, 2221
Hpy99I CGWCG 6 cut(s) 41, 197, 365, 380, 1911, 2036
HpyAV CCTTC 7 cut(s) 8, 244, 1154, 1667, 1963, 2011, 2271
HpyCH4III ACNGT 5 cut(s) 81, 963, 1720, 1831, 1874
HpyCH4IV ACGT 2 cut(s) 437, 1590
HpyF10VI GCNNNNNNNGC 6 cut(s) 439, 989, 1022, 1036, 1340, 1889
HpyF3I CTNAG 5 cut(s) 201, 616, 993, 1040, 1490
HpySE526I ACGT 2 cut(s) 437, 1590
Hsp92II CATG 8 cut(s) 265, 350, 872, 1713, 1924, 2053, 2125, 2236
HspAI GCGC 2 cut(s) 429, 431
Kpn2I TCCGGA 2 cut(s) 172, 335
Ksp22I TGATCA 3 cut(s) 1174, 2046, 2166
KspI CCGCGG 2 cut(s) 987, 1128
Kzo9I GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
LguI GCTCTTC 1 cut(s) 1519
LmnI GCTCC 8 cut(s) 103, 128, 194, 227, 1676, 1683, 2041, 2145
Lsp1109I GCAGC 7 cut(s) 231, 460, 813, 1001, 1009, 1355, 1466
LweI GCATC 4 cut(s) 1173, 1330, 1392, 1918
MaeI CTAG 6 cut(s) 186, 968, 1134, 1265, 1532, 2186
MaeII ACGT 2 cut(s) 437, 1590
MaeIII GTNAC 3 cut(s) 781, 963, 2202
MalI GATC 9 cut(s) 35, 343, 528, 888, 1176, 1773, 2048, 2156, 2168
MbiI CCGCTC 1 cut(s) 1487
MboI GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
MfeI CAATTG 1 cut(s) 1893
MflI RGATCY 1 cut(s) 526
MhlI GDGCHC 4 cut(s) 125, 1041, 1531, 1673
MlyI GAGTC 3 cut(s) 463, 1129, 1800
MmeI TCCRAC 5 cut(s) 216, 249, 342, 903, 2274
Mph1103I ATGCAT 1 cut(s) 1513
MroI TCCGGA 2 cut(s) 172, 335
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 9 cut(s) 506, 671, 846, 1256, 1430, 1515, 1586, 1664, 1823
MspA1I CMGCKG 5 cut(s) 535, 986, 992, 1127, 1562
MspI CCGG 6 cut(s) 151, 173, 336, 1169, 1243, 2200
MspR9I CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
MunI CAATTG 1 cut(s) 1893
MvaI CCWGG 4 cut(s) 250, 280, 1348, 1377
MvnI CGCG 4 cut(s) 431, 986, 1035, 1127
MwoI GCNNNNNNNGC 6 cut(s) 439, 989, 1022, 1036, 1340, 1889
NciI CCSGG 1 cut(s) 1169
NdeII GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
NlaIII CATG 8 cut(s) 265, 350, 872, 1713, 1924, 2053, 2125, 2236
NlaIV GGNNCC 5 cut(s) 52, 124, 940, 1252, 2147
NmuCI GTSAC 2 cut(s) 781, 963
NruI TCGCGA 1 cut(s) 1035
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 2 cut(s) 1713, 2125
PaeR7I CTCGAG 1 cut(s) 1391
PagI TCATGA 1 cut(s) 2049
PauI GCGCGC 1 cut(s) 429
PciSI GCTCTTC 1 cut(s) 1519
PcsI WCGNNNNNNNCGW 2 cut(s) 381, 882
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 5 cut(s) 143, 496, 790, 997, 1325
PflFI GACNNNGTC 1 cut(s) 274
PflMI CCANNNNNTGG 1 cut(s) 1145
PinAI ACCGGT 1 cut(s) 2199
Ple19I CGATCG 2 cut(s) 344, 889
PleI GAGTC 3 cut(s) 462, 1128, 1799
PpsI GAGTC 3 cut(s) 462, 1128, 1799
Psp124BI GAGCTC 2 cut(s) 1041, 1673
Psp6I CCWGG 4 cut(s) 248, 278, 1346, 1375
PspFI CCCAGC 1 cut(s) 1674
PspGI CCWGG 4 cut(s) 248, 278, 1346, 1375
PspN4I GGNNCC 5 cut(s) 52, 124, 940, 1252, 2147
PspPI GGNCC 3 cut(s) 938, 1251, 2020
PspXI VCTCGAGB 1 cut(s) 1391
PstNI CAGNNNCTG 2 cut(s) 74, 1145
PsuI RGATCY 1 cut(s) 526
PsyI GACNNNGTC 1 cut(s) 274
PteI GCGCGC 1 cut(s) 429
PvuI CGATCG 2 cut(s) 344, 889
PvuII CAGCTG 2 cut(s) 992, 1562
RruI TCGCGA 1 cut(s) 1035
RsaI GTAC 3 cut(s) 410, 2030, 2198
RsaNI GTAC 3 cut(s) 409, 2029, 2197
SacI GAGCTC 2 cut(s) 1041, 1673
SacII CCGCGG 2 cut(s) 987, 1128
SapI GCTCTTC 1 cut(s) 1519
SaqAI TTAA 9 cut(s) 506, 671, 846, 1256, 1430, 1515, 1586, 1664, 1823
Sau3AI GATC 9 cut(s) 33, 341, 526, 886, 1174, 1771, 2046, 2154, 2166
Sau96I GGNCC 3 cut(s) 938, 1251, 2020
SchI GAGTC 3 cut(s) 463, 1129, 1800
ScrFI CCNGG 5 cut(s) 250, 280, 1169, 1348, 1377
SduI GDGCHC 4 cut(s) 125, 1041, 1531, 1673
SfaNI GCATC 4 cut(s) 1173, 1330, 1392, 1918
SfcI CTRYAG 1 cut(s) 55
Sfr274I CTCGAG 1 cut(s) 1391
Sfr303I CCGCGG 2 cut(s) 987, 1128
SgrBI CCGCGG 2 cut(s) 987, 1128
SinI GGWCC 1 cut(s) 2020
SlaI CTCGAG 1 cut(s) 1391
SmlI CTYRAG 3 cut(s) 1329, 1391, 1634
SmoI CTYRAG 3 cut(s) 1329, 1391, 1634
SspMI CTAG 6 cut(s) 186, 968, 1134, 1265, 1532, 2186
SstI GAGCTC 2 cut(s) 1041, 1673
StyD4I CCNGG 5 cut(s) 248, 278, 1167, 1346, 1375
StyI CCWWGG 2 cut(s) 101, 1887
TaaI ACNGT 5 cut(s) 81, 963, 1720, 1831, 1874
TaiI ACGT 2 cut(s) 440, 1593
TaqI TCGA 8 cut(s) 36, 288, 375, 885, 889, 1392, 2157, 2214
TatI WGTACW 1 cut(s) 408
TauI GCSGC 3 cut(s) 332, 986, 989
TfiI GAWTC 5 cut(s) 143, 496, 790, 997, 1325
Tru1I TTAA 9 cut(s) 506, 671, 846, 1256, 1430, 1515, 1586, 1664, 1823
Tru9I TTAA 9 cut(s) 506, 671, 846, 1256, 1430, 1515, 1586, 1664, 1823
TscAI CASTG 4 cut(s) 452, 1048, 1879, 2030
TseFI GTSAC 2 cut(s) 781, 963
TseI GCWGC 7 cut(s) 219, 473, 826, 989, 1022, 1343, 1454
Tsp45I GTSAC 2 cut(s) 781, 963
TspDTI ATGAA 8 cut(s) 1173, 1284, 1437, 1584, 1631, 1829, 1997, 2123
TspGWI ACGGA 1 cut(s) 102
TspRI CASTG 4 cut(s) 452, 1048, 1879, 2030
Tth111I GACNNNGTC 1 cut(s) 274
Van91I CCANNNNNTGG 1 cut(s) 1145
VpaK11BI GGWCC 1 cut(s) 2020
XagI CCTNNNNNAGG 1 cut(s) 296
XapI RAATTY 5 cut(s) 549, 597, 628, 891, 1282
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 2 cut(s) 1713, 2125
XhoI CTCGAG 1 cut(s) 1391
XmnI GAANNNNTTC 1 cut(s) 396
XspI CTAG 6 cut(s) 186, 968, 1134, 1265, 1532, 2186
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.