Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Forward (+)

77283789 .. 77289489

Gene Structure 5701 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG484300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2823 bp

ATGGATTTTTCGTTGAAGAAGTCATTCAAATCCCATGGCTCGAGCAAACACATGAGAAAGATCTCAGCCGGAGCAGTAGAAGATAACAGCCACGAGGAGCTCCCGATTCTAATTGATCACCAGAACCAGGCAAGCATCGCCTCAGATTCGCCGGGCCACCACCGCCGAGAGGTCATCGTCAAGATTGACGAGGGCGATTCCTCCGCAACAACCATCAGAGAAAACGTCAGGGAAGTGGAGCATGGCGGGACGATTTGGCGGGAATCGAGTTTAGATTTTTGGACTGAGGACGAAGGGAGCACTATCCCTGCCGATGGAAGCGCCGGCAGCGGGGGCTTTCATTTCGTGCAGAGGGGGAAGCAGACTGCGCCGCCGACGGCTGAGGAGGATCCGCCGTCGAAGCTGATAGGGCAGTACCTTAAGAAGCAGAGAGCCTCGGGCGGCGACGTGTCGTTGGACATGGATTTGGAGATGGACGAGCTACAAAAACCGCCGCTGGCGGAGCCTCCGAGGATCAGCGAGGCGTCGCGGGAGCTCAAGGTGTCGTTCCAATCGCCGGCAAGGTCGGAGATCCCGATCCCCGAGACGGGGAACGATTCTGTTCGGAGGCGAAACAAGGACTCCGTGGTTGACGACGAACGACGTCGTAGCAGTCGGTTGAGTAACGGCGGAGCCGACGTCATTAGGTGCTCGTCGAACAAATCGTTTCGGCGAGAGGCTTCGTTTCAGAACAACAATTTGTTGAGGCTAAAGACCAAGTCTAGATTGATAGACCCTCCTGAAAGCTGGGACGAGGTTCGGGTCCCGAAATCGGGTCCGATGAAGTCCGGGATGTTGGGGAAAGGCGGCGGCGGTGACGATGACGACGAGGACCCGTTTTTGGAAGAGGATCTGCCTGATGAGTACAAGAGGGTAAAATTAGATGCATTGACTCTGCTCCAATGGCTTAGTTTGGTTTTGATCATTGGTGCCTTGGTTTGCACGCTTTCTCTAAGAGTTTTGAGGCAGAAGCATCTGTGGAAATTGAAGTTGTGGAAATGGGAGGTGCTGATTTTGGTTTCGATTTGCGGGAGGCTGGTTTCGGGTTGGGTGATAAAGATCATGGTGTTCTTCGTGGAAAGGAATTTCCTTCTGCGCAAGAGGGTGTTGTATTTTGTTTATGGGATCAGAAGGGCTGTGCAGAACTGTATTTGGCTTGGCTTTGTTTTGACGGCTTGGCATTTCATGCTAATCAGGAAGGTTGAGCAGGAAACCAATAGCAAAATACTAGAGTATGTGACGAAAGGTTTGCTTTGTCTCTTGATCGGGGTTTTGCTATGGTTGTTAAAGACCCTGGTTGTGAAGGTGCTTGCTTCCTCTTTCCACGTTAGATCCTACTTTGATCGGATTCAGGACGCGCTTTTTAATCAGTATGTGATCCAAACTCTCTCTGGGCGTCCCTTGATTGAGGTACAGAATGCGGAGGATGAAGAAGAGAGGCTTGCTGAGGAGGTTAGGAAGTTACAGAATGCTGGGGCAACTATGCCTCCTGACCTCAGATCAAATGCATTCCCTTCTGCGAGAATTGGGAGGGCGGTTGGGAGTGGCTCTGGGAGAAGTGGGAGGTTCATTGCTGCAAGTGGGGCGCTTGGGGGAAAAAGTGGCAAGTTTTCAAGACCAACAATGTCCAAGAAGTCAGAGGAGGCTGCAATTACCATTGATCATTTGCATAGGCTCAATCCAAAAAATGTATCTGCTTGGAATATGAAGAGGCTAATGAACATTGTTAGAAAAGGGCATCTTACGACACTCGATGAGCAAATTCTGGATTCCAGCGCTCAGGATGAGGGTGATACTATGATCAAAAGTGAAGTTGAGGCAAAGGCTGCAGCTAAAAAGATATTTCGCAATGTGGCACGGCCTGGCTCAAAGCACATCTACCTGGAGGATTTGTTGCGTTTCATGGGAGAAGAGGAGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCCGAGACTCGCAGAATCCGCAAAAATTCCTTGAAAAATTGGGTGGTCAACTGCTTTAGAGAACGGAGAGCACTTTCTTTTACACTGAATGACACCAAAACAGCGGTGAACAGACTTCGACACGTGGTGGATGTAATAGTTGGCATTGTTATTATAGTTATATGGCTTCTTGTGCTGGATATTGTCACCAGCGCCAATCTTGTTTATGCAACTTCTCAGCTTGTGGTCGTGGCATTCGTTTTTGGTAACACTTGCAGGACAGTATTTGAAGCAGTCATATTTTTATTTGTTATGCACCCATTTGACGTGGGAGATCGATGTGAAATTCAAGGAGTACAGATGGTTGTAGAAGAAATGAATATCTTGACTACAGTTTTTCTAAGGTACGACAACATAAAAATCGTGTACCCGAACAGTACACTTTCAACTATGCCCATCAATAACTTCTATCGTAGTCCGGACACAGGAGATGCTGTTGAGTTCTGTGTCCATATATGTACTCCACAAGATAAGATTGCTGTTATGAAACAGAGAATGATAAGTTACATTGAAAGTAAAAAGGAGCACTGGTATCCTCAAGTACAGATCGTATTGAAGGATGTAGAAGAATTAAATAAGATGAGGTATGCATTGTGGCTAACACATAAGATGAACTTCCAAGATGTGGGAGAAAAGTGGGAAAGAAGAGCCCTTTTACTTGAAGAGATGGTAAGGATCTTCCAGGAGCTGGACATTCAGTATCGTTTGCTGCCTATTGACATAAATGTCCGCGCCATGCCTCCCACAACCACCACCGGTCTGCCTCCCTCAAATTTTTCAGCAACCACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

940

Amino Acids

106.53

Weight (kDa)

8.78

Isoelectric Point (pI)

47.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	756 - 812	6.8e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2753
AatII	GACGTC	2 cut(s)	646, 681
Acc16I	TGCGCA	1 cut(s)	1136
AccB1I	GGYRCC	1 cut(s)	968
AccB7I	CCANNNNNTGG	2 cut(s)	2653, 2716
AccII	CGCG	3 cut(s)	529, 1397, 2760
AccIII	TCCGGA	1 cut(s)	2446
AcsI	RAATTY	5 cut(s)	1123, 1800, 2014, 2313, 2800
AcvI	CACGTG	1 cut(s)	2113
AcyI	GRCGYC	4 cut(s)	524, 643, 678, 1435
AdeI	CACNNNGTG	1 cut(s)	2116
AfaI	GTAC	9 cut(s)	416, 905, 1452, 2325, 2375, 2396, 2407, 2488, 2571
AfeI	AGCGCT	1 cut(s)	1816
AflII	CTTAAG	1 cut(s)	419
AflIII	ACRYGT	2 cut(s)	447, 2110
AgeI	ACCGGT	1 cut(s)	2783
AhdI	GACNNNNNGTC	1 cut(s)	1971
AjiI	CACGTC	2 cut(s)	448, 2296
AjnI	CCWGG	5 cut(s)	126, 1332, 1900, 1920, 2709
AluBI	AGCT	9 cut(s)	100, 403, 481, 535, 786, 1871, 2209, 2716, 2820
AluI	AGCT	9 cut(s)	100, 403, 481, 535, 786, 1871, 2209, 2716, 2820
Alw21I	GWGCWC	6 cut(s)	102, 302, 537, 692, 2062, 2556
Alw26I	GTCTC	4 cut(s)	578, 1301, 1979, 1988
AlwNI	CAGNNNCTG	1 cut(s)	2716
Ama87I	CYCGRG	3 cut(s)	40, 436, 581
Aor13HI	TCCGGA	1 cut(s)	2446
Aor51HI	AGCGCT	1 cut(s)	1816
AoxI	GGCC	3 cut(s)	154, 1898, 1986
ApeKI	GCWGC	6 cut(s)	327, 1613, 1685, 1865, 1868, 2737
ApoI	RAATTY	5 cut(s)	1123, 1800, 2014, 2313, 2800
ArsI	GACNNNNNNTTYG	2 cut(s)	1270, 1302
AsiGI	ACCGGT	1 cut(s)	2783
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	8 cut(s)	323, 370, 1137, 1399, 1627, 1817, 2183, 2762
AspS9I	GGNCC	5 cut(s)	154, 802, 815, 871, 1986
AsuC2I	CCSGG	2 cut(s)	153, 829
AsuHPI	GGTGA	6 cut(s)	110, 866, 1102, 1841, 2107, 2167
AvaI	CYCGRG	3 cut(s)	40, 436, 581
AvaII	GGWCC	3 cut(s)	802, 815, 871
BamHI	GGATCC	1 cut(s)	388
BanI	GGYRCC	1 cut(s)	968
BanII	GRGCYC	3 cut(s)	102, 537, 2680
BauI	CACGAG	1 cut(s)	92
BbrPI	CACGTG	1 cut(s)	2113
Bbv12I	GWGCWC	6 cut(s)	102, 302, 537, 692, 2062, 2556
BbvCI	CCTCAGC	2 cut(s)	381, 1485
BbvI	GCAGC	6 cut(s)	339, 1600, 1672, 1852, 1880, 2724
BccI	CCATC	6 cut(s)	221, 308, 466, 2323, 2432, 2689
BceAI	ACGGC	5 cut(s)	379, 393, 682, 1227, 1913
BcgI	CGANNNNNNTGC	2 cut(s)	1270, 1304
BciT130I	CCWGG	5 cut(s)	128, 1334, 1902, 1922, 2711
BciVI	GTATCC	1 cut(s)	2571
BclI	TGATCA	4 cut(s)	115, 960, 1699, 1839
BcnI	CCSGG	2 cut(s)	153, 829
BcoDI	GTCTC	4 cut(s)	578, 1301, 1979, 1988
BfaI	CTAG	2 cut(s)	762, 1268
BfmI	CTRYAG	2 cut(s)	1866, 2358
BfoI	RGCGCY	4 cut(s)	324, 1628, 1818, 2184
BfrI	CTTAAG	1 cut(s)	419
BfuI	GTATCC	1 cut(s)	2571
BglII	AGATCT	1 cut(s)	60
Bme1390I	CCNGG	7 cut(s)	128, 153, 829, 1334, 1902, 1922, 2711
Bme18I	GGWCC	3 cut(s)	802, 815, 871
BmeRI	GACNNNNNGTC	1 cut(s)	1971
BmeT110I	CYCGRG	3 cut(s)	40, 436, 581
BmgBI	CACGTC	2 cut(s)	448, 2296
BmgT120I	GGNCC	5 cut(s)	154, 802, 815, 871, 1986
BmiI	GGNNCC	9 cut(s)	390, 504, 673, 803, 804, 816, 873, 970, 1987
BmrFI	CCNGG	7 cut(s)	128, 153, 829, 1334, 1902, 1922, 2711
BmsI	GCATC	5 cut(s)	144, 913, 1021, 1786, 2449
BpmI	CTGGAG	1 cut(s)	1943
Bpu10I	CCTNAGC	3 cut(s)	381, 1485, 1818
BpuEI	CTTGAG	2 cut(s)	521, 2550
BpuMI	CCSGG	2 cut(s)	153, 829
Bsa29I	ATCGAT	1 cut(s)	2305
BsaAI	YACGTR	1 cut(s)	2113
BsaBI	GATNNNNATC	2 cut(s)	575, 1097
BsaHI	GRCGYC	4 cut(s)	524, 643, 678, 1435
BsaJI	CCNNGG	6 cut(s)	34, 435, 509, 624, 972, 1332
BsaWI	WCCGGW	2 cut(s)	2446, 2783
Bse118I	RCCGGY	3 cut(s)	323, 556, 2783
Bse1I	ACTGG	1 cut(s)	2561
Bse3DI	GCAATG	2 cut(s)	1608, 1894
Bse8I	GATNNNNATC	2 cut(s)	575, 1097
BseAI	TCCGGA	1 cut(s)	2446
BseBI	CCWGG	5 cut(s)	128, 1334, 1902, 1922, 2711
BseCI	ATCGAT	1 cut(s)	2305
BseDI	CCNNGG	6 cut(s)	34, 435, 509, 624, 972, 1332
BseGI	GGATG	5 cut(s)	837, 1471, 1828, 2125, 2593
BseJI	GATNNNNATC	2 cut(s)	575, 1097
BseMI	GCAATG	2 cut(s)	1608, 1894
BseMII	CTCAG	8 cut(s)	78, 156, 276, 372, 1476, 1549, 1832, 2219
BseNI	ACTGG	1 cut(s)	2561
BseRI	GAGGAG	5 cut(s)	110, 398, 1502, 1694, 1967
BseXI	GCAGC	6 cut(s)	339, 1600, 1672, 1852, 1880, 2724
BseYI	CCCAGC	2 cut(s)	786, 1511
BsgI	GTGCAG	2 cut(s)	368, 1199
Bsh1236I	CGCG	3 cut(s)	529, 1397, 2760
BshFI	GGCC	3 cut(s)	156, 1900, 1988
BshNI	GGYRCC	1 cut(s)	968
BshTI	ACCGGT	1 cut(s)	2783
BshVI	ATCGAT	1 cut(s)	2305
BsiHKAI	GWGCWC	6 cut(s)	102, 302, 537, 692, 2062, 2556
BsiHKCI	CYCGRG	3 cut(s)	40, 436, 581
BsiSI	CCGG	7 cut(s)	69, 152, 324, 557, 828, 2447, 2784
BslFI	GGGAC	4 cut(s)	262, 788, 803, 1422
BsmAI	GTCTC	4 cut(s)	578, 1301, 1979, 1988
BsmBI	CGTCTC	1 cut(s)	578
BsmFI	GGGAC	4 cut(s)	262, 788, 803, 1422
BsmI	GAATGC	4 cut(s)	1462, 1513, 1547, 2222
BsnI	GGCC	3 cut(s)	156, 1900, 1988
BsoBI	CYCGRG	3 cut(s)	40, 436, 581
Bsp1286I	GDGCHC	7 cut(s)	102, 302, 537, 692, 2062, 2556, 2680
Bsp13I	TCCGGA	1 cut(s)	2446
Bsp19I	CCATGG	1 cut(s)	34
BspANI	GGCC	3 cut(s)	156, 1900, 1988
BspCNI	CTCAG	8 cut(s)	77, 155, 277, 373, 1477, 1548, 1831, 2218
BspDI	ATCGAT	1 cut(s)	2305
BspEI	TCCGGA	1 cut(s)	2446
BspFNI	CGCG	3 cut(s)	529, 1397, 2760
BspLI	GGNNCC	9 cut(s)	390, 504, 673, 803, 804, 816, 873, 970, 1987
BspMAI	CTGCAG	1 cut(s)	1870
BspQI	GCTCTTC	1 cut(s)	2668
BspT107I	GGYRCC	1 cut(s)	968
BspTI	CTTAAG	1 cut(s)	419
BsrDI	GCAATG	2 cut(s)	1608, 1894
BsrFI	RCCGGY	3 cut(s)	323, 556, 2783
BsrI	ACTGG	1 cut(s)	2561
BssAI	RCCGGY	3 cut(s)	323, 556, 2783
BssECI	CCNNGG	6 cut(s)	34, 435, 509, 624, 972, 1332
BssNI	GRCGYC	4 cut(s)	524, 643, 678, 1435
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	2 cut(s)	34, 972
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	5 cut(s)	128, 1334, 1902, 1922, 2711
Bst4CI	ACNGT	4 cut(s)	1187, 2251, 2362, 2405
Bst6I	CTCTTC	6 cut(s)	879, 1467, 1742, 1944, 2668, 2685
BstACI	GRCGYC	4 cut(s)	524, 643, 678, 1435
BstAFI	CTTAAG	1 cut(s)	419
BstAPI	GCANNNNNTGC	2 cut(s)	1225, 1865
BstBAI	YACGTR	1 cut(s)	2113
BstC8I	GCNNGC	7 cut(s)	133, 325, 498, 558, 983, 1350, 1482
BstDSI	CCRYGG	2 cut(s)	34, 624
BstF5I	GGATG	5 cut(s)	837, 1471, 1828, 2125, 2593
BstFNI	CGCG	3 cut(s)	529, 1397, 2760
BstH2I	RGCGCY	4 cut(s)	324, 1628, 1818, 2184
BstHHI	GCGC	8 cut(s)	323, 370, 1137, 1399, 1627, 1817, 2183, 2762
BstMAI	GTCTC	4 cut(s)	578, 1301, 1979, 1988
BstNI	CCWGG	5 cut(s)	128, 1334, 1902, 1922, 2711
BstSCI	CCNGG	7 cut(s)	126, 151, 827, 1332, 1900, 1920, 2709
BstSFI	CTRYAG	2 cut(s)	1866, 2358
BstUI	CGCG	3 cut(s)	529, 1397, 2760
BstV1I	GCAGC	6 cut(s)	339, 1600, 1672, 1852, 1880, 2724
BstX2I	RGATCY	6 cut(s)	60, 388, 570, 889, 1370, 2703
BstYI	RGATCY	6 cut(s)	60, 388, 570, 889, 1370, 2703
Bsu15I	ATCGAT	1 cut(s)	2305
BsuI	GTATCC	1 cut(s)	2571
BsuRI	GGCC	3 cut(s)	156, 1900, 1988
BsuTUI	ATCGAT	1 cut(s)	2305
BtgI	CCRYGG	2 cut(s)	34, 624
BtgZI	GCGATG	1 cut(s)	121
BtrI	CACGTC	2 cut(s)	448, 2296
BtsCI	GGATG	5 cut(s)	837, 1471, 1828, 2125, 2593
BtsIMutI	CAGTG	2 cut(s)	2072, 2554
Cac8I	GCNNGC	7 cut(s)	133, 325, 498, 558, 983, 1350, 1482
CaiI	CAGNNNCTG	1 cut(s)	2716
CfoI	GCGC	8 cut(s)	323, 370, 1137, 1399, 1627, 1817, 2183, 2762
Cfr10I	RCCGGY	3 cut(s)	323, 556, 2783
Cfr13I	GGNCC	5 cut(s)	154, 802, 815, 871, 1986
ClaI	ATCGAT	1 cut(s)	2305
CseI	GACGC	3 cut(s)	513, 1403, 1424
Csp6I	GTAC	9 cut(s)	415, 904, 1451, 2324, 2374, 2395, 2406, 2487, 2570
CspAI	ACCGGT	1 cut(s)	2783
CviAII	CATG	9 cut(s)	35, 52, 242, 460, 1102, 1225, 1942, 1969, 2764
CviQI	GTAC	9 cut(s)	415, 904, 1451, 2324, 2374, 2395, 2406, 2487, 2570
DraI	TTTAAA	1 cut(s)	1962
DraIII	CACNNNGTG	1 cut(s)	2116
DrdI	GACNNNNNNGTC	1 cut(s)	2753
DriI	GACNNNNNGTC	1 cut(s)	1971
DseDI	GACNNNNNNGTC	1 cut(s)	2753
Eam1104I	CTCTTC	6 cut(s)	879, 1467, 1742, 1944, 2668, 2685
Eam1105I	GACNNNNNGTC	1 cut(s)	1971
EarI	CTCTTC	6 cut(s)	879, 1467, 1742, 1944, 2668, 2685
EciI	GGCGGA	3 cut(s)	381, 515, 684
Ecl136II	GAGCTC	2 cut(s)	100, 535
Eco130I	CCWWGG	2 cut(s)	34, 972
Eco24I	GRGCYC	3 cut(s)	102, 537, 2680
Eco47I	GGWCC	3 cut(s)	802, 815, 871
Eco47III	AGCGCT	1 cut(s)	1816
Eco53kI	GAGCTC	2 cut(s)	100, 535
Eco72I	CACGTG	1 cut(s)	2113
Eco88I	CYCGRG	3 cut(s)	40, 436, 581
EcoICRI	GAGCTC	2 cut(s)	100, 535
EcoO109I	RGGNCCY	3 cut(s)	802, 871, 1986
EcoRII	CCWGG	5 cut(s)	126, 1332, 1900, 1920, 2709
EcoT14I	CCWWGG	2 cut(s)	34, 972
EcoT22I	ATGCAT	3 cut(s)	928, 1549, 2620
EcoT38I	GRGCYC	3 cut(s)	102, 537, 2680
ErhI	CCWWGG	2 cut(s)	34, 972
Esp3I	CGTCTC	1 cut(s)	578
FaeI	CATG	9 cut(s)	38, 55, 245, 463, 1105, 1228, 1945, 1972, 2767
FalI	AAGNNNNNCTT	8 cut(s)	1275, 1307, 1464, 1496, 1764, 1796, 2627, 2659
FaqI	GGGAC	4 cut(s)	262, 788, 803, 1422
FatI	CATG	9 cut(s)	34, 51, 241, 459, 1101, 1224, 1941, 1968, 2763
FauI	CCCGC	5 cut(s)	239, 252, 323, 522, 1061
FbaI	TGATCA	4 cut(s)	115, 960, 1699, 1839
FokI	GGATG	5 cut(s)	844, 1478, 1835, 2132, 2600
FriOI	GRGCYC	3 cut(s)	102, 537, 2680
FspBI	CTAG	2 cut(s)	762, 1268
FspI	TGCGCA	1 cut(s)	1136
GlaI	GCGC	8 cut(s)	322, 369, 1136, 1398, 1626, 1816, 2182, 2761
GsaI	CCCAGC	2 cut(s)	790, 1515
GsuI	CTGGAG	1 cut(s)	1943
HaeII	RGCGCY	4 cut(s)	324, 1628, 1818, 2184
HaeIII	GGCC	3 cut(s)	156, 1900, 1988
HapII	CCGG	7 cut(s)	69, 152, 324, 557, 828, 2447, 2784
HgaI	GACGC	3 cut(s)	513, 1403, 1424
HhaI	GCGC	8 cut(s)	323, 370, 1137, 1399, 1627, 1817, 2183, 2762
Hin1I	GRCGYC	4 cut(s)	524, 643, 678, 1435
Hin1II	CATG	9 cut(s)	38, 55, 245, 463, 1105, 1228, 1945, 1972, 2767
Hin6I	GCGC	8 cut(s)	321, 368, 1135, 1397, 1625, 1815, 2181, 2760
HinP1I	GCGC	8 cut(s)	321, 368, 1135, 1397, 1625, 1815, 2181, 2760
HincII	GTYRAC	2 cut(s)	631, 2038
HindII	GTYRAC	2 cut(s)	631, 2038
HpaII	CCGG	7 cut(s)	69, 152, 324, 557, 828, 2447, 2784
HphI	GGTGA	6 cut(s)	110, 866, 1102, 1841, 2107, 2167
Hpy166II	GTNNAC	5 cut(s)	631, 2038, 2098, 2395, 2408
Hpy8I	GTNNAC	5 cut(s)	631, 2038, 2098, 2395, 2408
HpyAV	CCTTC	8 cut(s)	287, 1139, 1164, 1231, 1336, 1563, 1976, 2578
HpyCH4III	ACNGT	4 cut(s)	1187, 2251, 2362, 2405
HpyCH4IV	ACGT	7 cut(s)	225, 447, 643, 678, 1365, 2112, 2295
HpySE526I	ACGT	7 cut(s)	225, 447, 643, 678, 1365, 2112, 2295
Hsp92I	GRCGYC	4 cut(s)	524, 643, 678, 1435
Hsp92II	CATG	9 cut(s)	38, 55, 245, 463, 1105, 1228, 1945, 1972, 2767
HspAI	GCGC	8 cut(s)	321, 368, 1135, 1397, 1625, 1815, 2181, 2760
KflI	GGGWCCC	1 cut(s)	802
Kpn2I	TCCGGA	1 cut(s)	2446
KroI	GCCGGC	2 cut(s)	323, 556
KroNI	GCCGGC	2 cut(s)	325, 558
Ksp22I	TGATCA	4 cut(s)	115, 960, 1699, 1839
LguI	GCTCTTC	1 cut(s)	2668
Lsp1109I	GCAGC	6 cut(s)	339, 1600, 1672, 1852, 1880, 2724
LweI	GCATC	5 cut(s)	144, 913, 1021, 1786, 2449
MaeI	CTAG	2 cut(s)	762, 1268
MaeII	ACGT	7 cut(s)	225, 447, 643, 678, 1365, 2112, 2295
MaeIII	GTNAC	7 cut(s)	662, 854, 1276, 1500, 2173, 2234, 2531
MflI	RGATCY	6 cut(s)	60, 388, 570, 889, 1370, 2703
MhlI	GDGCHC	7 cut(s)	102, 302, 537, 692, 2062, 2556, 2680
MlyI	GAGTC	4 cut(s)	614, 925, 1981, 1990
MmeI	TCCRAC	2 cut(s)	435, 546
Mph1103I	ATGCAT	3 cut(s)	928, 1549, 2620
MroI	TCCGGA	1 cut(s)	2446
MroNI	GCCGGC	2 cut(s)	323, 556
MroXI	GAANNNNTTC	1 cut(s)	23
MseI	TTAA	5 cut(s)	420, 1325, 1404, 1961, 2600
MspA1I	CMGCKG	3 cut(s)	330, 496, 2093
MspCI	CTTAAG	1 cut(s)	419
MspI	CCGG	7 cut(s)	69, 152, 324, 557, 828, 2447, 2784
MspR9I	CCNGG	7 cut(s)	128, 153, 829, 1334, 1902, 1922, 2711
Mva1269I	GAATGC	4 cut(s)	1462, 1513, 1547, 2222
MvaI	CCWGG	5 cut(s)	128, 1334, 1902, 1922, 2711
MvnI	CGCG	3 cut(s)	529, 1397, 2760
NaeI	GCCGGC	2 cut(s)	325, 558
NciI	CCSGG	2 cut(s)	153, 829
NcoI	CCATGG	1 cut(s)	34
NgoMIV	GCCGGC	2 cut(s)	323, 556
NlaIII	CATG	9 cut(s)	38, 55, 245, 463, 1105, 1228, 1945, 1972, 2767
NlaIV	GGNNCC	9 cut(s)	390, 504, 673, 803, 804, 816, 873, 970, 1987
NmeAIII	GCCGAG	1 cut(s)	191
NmuCI	GTSAC	3 cut(s)	854, 1276, 2173
NsbI	TGCGCA	1 cut(s)	1136
NsiI	ATGCAT	3 cut(s)	928, 1549, 2620
PaeR7I	CTCGAG	1 cut(s)	40
PciSI	GCTCTTC	1 cut(s)	2668
PcsI	WCGNNNNNNNCGW	5 cut(s)	572, 672, 701, 864, 2223
PctI	GAATGC	4 cut(s)	1462, 1513, 1547, 2222
PdiI	GCCGGC	2 cut(s)	325, 558
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	8 cut(s)	106, 146, 197, 263, 596, 1387, 1808, 2004
PflFI	GACNNNGTC	1 cut(s)	757
PflMI	CCANNNNNTGG	2 cut(s)	2653, 2716
PfoI	TCCNGGA	2 cut(s)	827, 2709
PinAI	ACCGGT	1 cut(s)	2783
PleI	GAGTC	4 cut(s)	614, 925, 1980, 1990
PmaCI	CACGTG	1 cut(s)	2113
PmlI	CACGTG	1 cut(s)	2113
PpsI	GAGTC	4 cut(s)	614, 925, 1980, 1990
Ppu21I	YACGTR	1 cut(s)	2113
PpuMI	RGGWCCY	2 cut(s)	802, 871
Psp124BI	GAGCTC	2 cut(s)	102, 537
Psp5II	RGGWCCY	2 cut(s)	802, 871
Psp6I	CCWGG	5 cut(s)	126, 1332, 1900, 1920, 2709
PspCI	CACGTG	1 cut(s)	2113
PspFI	CCCAGC	2 cut(s)	786, 1511
PspGI	CCWGG	5 cut(s)	126, 1332, 1900, 1920, 2709
PspN4I	GGNNCC	9 cut(s)	390, 504, 673, 803, 804, 816, 873, 970, 1987
PspPI	GGNCC	5 cut(s)	154, 802, 815, 871, 1986
PspPPI	RGGWCCY	2 cut(s)	802, 871
PspXI	VCTCGAGB	1 cut(s)	40
PstI	CTGCAG	1 cut(s)	1870
PstNI	CAGNNNCTG	1 cut(s)	2716
PsuI	RGATCY	6 cut(s)	60, 388, 570, 889, 1370, 2703
PsyI	GACNNNGTC	1 cut(s)	757
RsaI	GTAC	9 cut(s)	416, 905, 1452, 2325, 2375, 2396, 2407, 2488, 2571
RsaNI	GTAC	9 cut(s)	415, 904, 1451, 2324, 2374, 2395, 2406, 2487, 2570
SacI	GAGCTC	2 cut(s)	102, 537
SapI	GCTCTTC	1 cut(s)	2668
SaqAI	TTAA	5 cut(s)	420, 1325, 1404, 1961, 2600
Sau96I	GGNCC	5 cut(s)	154, 802, 815, 871, 1986
SchI	GAGTC	4 cut(s)	614, 925, 1981, 1990
ScrFI	CCNGG	7 cut(s)	128, 153, 829, 1334, 1902, 1922, 2711
SduI	GDGCHC	7 cut(s)	102, 302, 537, 692, 2062, 2556, 2680
SfaNI	GCATC	5 cut(s)	144, 913, 1021, 1786, 2449
SfcI	CTRYAG	2 cut(s)	1866, 2358
Sfr274I	CTCGAG	1 cut(s)	40
SinI	GGWCC	3 cut(s)	802, 815, 871
SlaI	CTCGAG	1 cut(s)	40
SmlI	CTYRAG	4 cut(s)	40, 419, 536, 2565
SmoI	CTYRAG	4 cut(s)	40, 419, 536, 2565
SspMI	CTAG	2 cut(s)	762, 1268
SstI	GAGCTC	2 cut(s)	102, 537
StyD4I	CCNGG	7 cut(s)	126, 151, 827, 1332, 1900, 1920, 2709
StyI	CCWWGG	2 cut(s)	34, 972
TaaI	ACNGT	4 cut(s)	1187, 2251, 2362, 2405
TaiI	ACGT	7 cut(s)	228, 450, 646, 681, 1368, 2115, 2298
TaqI	TCGA	8 cut(s)	41, 266, 398, 695, 1061, 1791, 2107, 2305
TatI	WGTACW	5 cut(s)	903, 2323, 2405, 2486, 2569
TauI	GCSGC	5 cut(s)	373, 444, 496, 849, 852
TfiI	GAWTC	8 cut(s)	106, 146, 197, 263, 596, 1387, 1808, 2004
Tru1I	TTAA	5 cut(s)	420, 1325, 1404, 1961, 2600
Tru9I	TTAA	5 cut(s)	420, 1325, 1404, 1961, 2600
TscAI	CASTG	2 cut(s)	2079, 2561
TseFI	GTSAC	3 cut(s)	854, 1276, 2173
TseI	GCWGC	6 cut(s)	327, 1613, 1685, 1865, 1868, 2737
Tsp45I	GTSAC	3 cut(s)	854, 1276, 2173
TspGWI	ACGGA	2 cut(s)	613, 2068
TspRI	CASTG	2 cut(s)	2079, 2561
Tth111I	GACNNNGTC	1 cut(s)	757
Van91I	CCANNNNNTGG	2 cut(s)	2653, 2716
Vha464I	CTTAAG	1 cut(s)	419
VpaK11BI	GGWCC	3 cut(s)	802, 815, 871
XapI	RAATTY	5 cut(s)	1123, 1800, 2014, 2313, 2800
XbaI	TCTAGA	1 cut(s)	761
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1427
XhoI	CTCGAG	1 cut(s)	40
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	2 cut(s)	762, 1268
ZraI	GACGTC	2 cut(s)	644, 679
Zsp2I	ATGCAT	3 cut(s)	928, 1549, 2620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.