MD08G1006800.v1.1

Mechanosensitive ion channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001376
Physical Location & Seq
Reverse (-)
541867 .. 545441
3575 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1006800.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGGCAGCGAGGGAAGGACAAGTTCTACCCAAGATCGACGGCGGTGATGCTTCTTCCAGCAAAGATACAGAGACTGCACCAAATGTCGACGGAGAAGCAGCTCCAAGGTCGCCAATAATTCACGGCTCCCCAGAGAACCCTACTGAATCAGTCCAACGGAGAAGCAGAGATCTGCCGGGAGCTTCCTCCAGCCCGGGCGGAGCTCCCGAGATTGTCCGATGCAGCCCCAATGCCTCCCCCCGAAGCTCCTGGAAACCGCCGGTGAGCAAGACCAAGTCCAGGCTGTTCGACCCACCACCTGAGGAACCGTGTGTGAAATCGGACAGAGTTGCGGGCTCCGGACGAACTCCGCGGAGAGATGACGACGCTTCGGCTTTCGATGACGACGACCTCGAAGACATTCCAGATGAGTATAAGAAGATCAAGTTCGATGCCCTCACATTGGTTCAGTGGGTGAGTCTTGTTATCGTTATAGCTGCTTTAGTTTGCAATCTATGGATTCCAGTAATAAGGAAGAAAACTTTGTGGAATCTTCCGCTGTGGAAGTGGGAAATTCTGGTTCTGGCGTTGATTTGTGGTCGTTTGGTTTCGGGTTGGGGAATTCGGGTGATTGTGTTCCTTGTAGAGCGAAATTTCTTGTTGCGAAAACGGGTTTTGTATTTCGTATACGGTTTAAGGAGATCTGTTCAGAATTGTCTGTGGTTAGGATTGGTTTTGTTGGTATGGCGCTTGATTTTTGATAAGAAGGTGGAGGAGAAGACCAAGCATAGGATTTTGCCTTATGTGACCAAGATTTTGGTATGTTTTCTTTTTATGACATTGATTTGGCTGCTGAAGACAATACTTGTTAAGGTTCTTGCCTTGTATTTCCATGTCAACGCCTTTTTCGACCGGATTCAGGAGGCTTTGTTCACTCAGTATGTGTTTGAGACGCTATCTGGTCCCCCTATGTTTGAAAGGCAACACACACGGGAAGAGGAAAAGGCCGCGGCAGCTGAGATTAGGGAGTTTCAGAAGGCCGGGGTTGCTATGCCTCGTGATCTCAGGGCAACTCTTTTTCCGAGGACAAGGAGTGGGAGAGTTGTAGGGAGTGGAAGCCATAATAGCCCCAGAGTAGGGAAGAGTCCCGCGGTTTCTAGGCCAATGTCTGAGAGCCAAGATGAGGAAATCCCGGTTGATCACTTGCAAAAGCTAAATCAGAAGAATGTGTCGGCCTGGAGTATGAGGAGGATGGTAAACATCATACGGCACGGATCCTTAACCACTCTAGATGAACAAATACTGAACTCCAATGTGGGAGACGAGTCTTCTTTGAAGATTACAACTGAATGTCAAGCAAAAGAGGCAGCCAGGAAGATATTCATGAGAGTAGCCAAGCCAGGGTTCCCACACATTTGCCTGGAGGATTTGATGCGGTTTATGAATAAAGATGAAGCTTTGAAGACGCTTCATCTCTTTGGTGCAGCAAGTGAACATGACAAAATCGGGAAGACCGCTCTTAAAAATTGGGTGGTCAATGCATTTAAAGAAAGAAGAGCACTAGCATTGTCTTTGACTGATACAAAAACAGCTGTGGACGAACTCCATAAAATTATAAATGTCCTTGTATCTATTGTCATTGTGATAATCTGGCTAATTATTCTTGGGGTTCATGTCAGCCACTACCTTCTCTTAATAAGCTCCCAGCTTCTTTTGCTTGCATTCATCTTTGGGAACACATGTAAGATGGTTTTTGAAGCAATCATCTTTCTATTTGTAAGGCATCCGTTTGATGTGGGTGATCGCTGTGAAGTGGATGGAGTCCAGATGGTAGTTGAAGAGATGAATATATTAACTACAGTCTTTCTGAAGTATGATAACCAGAAGATCATATACCCTAACAGTGTTCTTGCGACCAAGGGAATTGCTAATTATCATCGTAGTCCTGACACGGTTGAATTTATTGATTTCTGTGTTCATATCGCAACTCCTATGGAGAAGGTGGCCACTATGAAAGAAAGACTAAAGAAGTATATGAAAAGCAGGAAGGATCACTGGCATGATGATCCGTTGCTGGTCACAAGGGATGTGCTGGATTTGAACAGACTGATGATATCGGTATGGCCGACACACAAGATGAACTATCAGGACATAGAGGAGAGATGGAGGAGGAGAAGCCTTTTGGTCGAAGAGATGATCAAAGTATTCAGAGAGCTCGATATTGAGTACCGGTTACTGCCTATTGACATCAACGTCCGAGCCATGCCAAGTTTAACCTCCGACAGACGTCCTTCAAATTGGACAACCAGTGCCCCGAAGCCAGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0016020 GO:0044464 GO:0051179 GO:0051234 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel

Protein Analysis

770

Amino Acids

87.78

Weight (kDa)

9.18

Isoelectric Point (pI)

50.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 582 - 640 1.8e-11 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1595
AasI GACNNNNNNGTC 1 cut(s) 2231
AatII GACGTC 1 cut(s) 2269
AccBSI CCGCTC 1 cut(s) 1496
AccI GTMKAC 2 cut(s) 87, 666
AccII CGCG 3 cut(s) 352, 989, 1130
AccIII TCCGGA 1 cut(s) 338
AclWI GGATC 4 cut(s) 1248, 1261, 2037, 2039
AcoI YGGCCR 2 cut(s) 1983, 2102
AcsI RAATTY 4 cut(s) 552, 600, 631, 1937
AcuI CTGAAG 2 cut(s) 854, 1868
AcyI GRCGYC 1 cut(s) 2266
AfaI GTAC 1 cut(s) 2207
AfiI CCNNNNNNNGG 8 cut(s) 279, 442, 768, 898, 1115, 1116, 1162, 1380
AflIII ACRYGT 1 cut(s) 1718
AgeI ACCGGT 1 cut(s) 2208
AgsI TTSAA 8 cut(s) 956, 1315, 1441, 1736, 1817, 1937, 2080, 2274
AjnI CCWGG 6 cut(s) 248, 278, 1214, 1349, 1378, 1398
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
AloI GAACNNNNNNTCC 2 cut(s) 893, 925
Alw21I GWGCWC 3 cut(s) 205, 1540, 2196
Alw26I GTCTC 3 cut(s) 65, 923, 1293
AlwI GGATC 4 cut(s) 1248, 1261, 2037, 2039
AlwNI CAGNNNCTG 1 cut(s) 74
Ama87I CYCGRG 2 cut(s) 193, 206
Aor13HI TCCGGA 1 cut(s) 338
AoxI GGCC 6 cut(s) 984, 1017, 1139, 1212, 1983, 2102
ApeKI GCWGC 8 cut(s) 5, 98, 222, 476, 829, 992, 1346, 1463
ApoI RAATTY 4 cut(s) 552, 600, 631, 1937
ArsI GACNNNNNNTTYG 2 cut(s) 778, 810
AsiGI ACCGGT 1 cut(s) 2208
Asp700I GAANNNNTTC 2 cut(s) 399, 1358
AspLEI GCGC 1 cut(s) 729
AspS9I GGNCC 1 cut(s) 941
AsuC2I CCSGG 5 cut(s) 177, 194, 195, 1021, 1172
AsuHPI GGTGA 5 cut(s) 56, 274, 466, 619, 1790
AvaI CYCGRG 2 cut(s) 193, 206
AvaII GGWCC 1 cut(s) 941
AxyI CCTNAGG 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 2293
BalI TGGCCA 1 cut(s) 1985
BamHI GGATCC 1 cut(s) 1253
BanII GRGCYC 3 cut(s) 205, 338, 2196
BarI GAAGNNNNNNTAC 2 cut(s) 1856, 1888
BauI CACGAG 1 cut(s) 1035
BbsI GAAGAC 6 cut(s) 402, 764, 842, 1299, 1448, 1496
Bbv12I GWGCWC 3 cut(s) 205, 1540, 2196
BbvI GCAGC 8 cut(s) 17, 110, 234, 463, 816, 1004, 1358, 1475
BccI CCATC 5 cut(s) 1225, 1720, 1790, 1801, 2136
BceAI ACGGC 3 cut(s) 55, 139, 1262
BciT130I CCWGG 6 cut(s) 250, 280, 1216, 1351, 1380, 1400
BclI TGATCA 2 cut(s) 1177, 2175
BcnI CCSGG 5 cut(s) 177, 194, 195, 1021, 1172
BcoDI GTCTC 3 cut(s) 65, 923, 1293
BfaI CTAG 3 cut(s) 1137, 1268, 1541
BfmI CTRYAG 1 cut(s) 1836
BfoI RGCGCY 1 cut(s) 730
BglII AGATCT 2 cut(s) 169, 680
Bme18I GGWCC 1 cut(s) 941
BmeT110I CYCGRG 2 cut(s) 193, 206
BmgT120I GGNCC 1 cut(s) 941
BmiI GGNNCC 6 cut(s) 127, 306, 337, 943, 1255, 1385
BmsI GCATC 5 cut(s) 37, 209, 421, 1401, 1771
BoxI GACNNNNGTC 1 cut(s) 2265
BpiI GAAGAC 6 cut(s) 402, 764, 842, 1299, 1448, 1496
BpmI CTGGAG 3 cut(s) 172, 1237, 1421
BpuMI CCSGG 5 cut(s) 177, 194, 195, 1021, 1172
BsaHI GRCGYC 1 cut(s) 2266
BsaJI CCNNGG 9 cut(s) 104, 193, 350, 987, 1020, 1061, 1128, 1379, 1896
BsaWI WCCGGW 3 cut(s) 338, 891, 2208
BsaXI ACNNNNNCTCC 2 cut(s) 893, 923
Bsc4I CCNNNNNNNGG 8 cut(s) 279, 442, 768, 898, 1115, 1116, 1162, 1380
Bse118I RCCGGY 2 cut(s) 259, 2208
Bse1I ACTGG 3 cut(s) 503, 2039, 2286
Bse21I CCTNAGG 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 338
BseBI CCWGG 6 cut(s) 250, 280, 1216, 1351, 1380, 1400
BseDI CCNNGG 9 cut(s) 104, 193, 350, 987, 1020, 1061, 1128, 1379, 1896
BseGI GGATG 4 cut(s) 1236, 1762, 1801, 2071
BseLI CCNNNNNNNGG 8 cut(s) 279, 442, 768, 898, 1115, 1116, 1162, 1380
BseMII CTCAG 5 cut(s) 291, 929, 987, 1057, 1140
BseNI ACTGG 3 cut(s) 503, 2039, 2286
BseRI GAGGAG 5 cut(s) 767, 1240, 2150, 2161, 2164
BseSI GKGCMC 1 cut(s) 2293
BseXI GCAGC 8 cut(s) 17, 110, 234, 463, 816, 1004, 1358, 1475
BseYI CCCAGC 1 cut(s) 1683
BsgI GTGCAG 2 cut(s) 60, 1482
Bsh1236I CGCG 3 cut(s) 352, 989, 1130
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 6 cut(s) 986, 1019, 1141, 1214, 1985, 2104
BshTI ACCGGT 1 cut(s) 2208
BsiEI CGRYCG 1 cut(s) 892
BsiHKAI GWGCWC 3 cut(s) 205, 1540, 2196
BsiHKCI CYCGRG 2 cut(s) 193, 206
BsiSI CCGG 8 cut(s) 176, 194, 260, 339, 892, 1020, 1172, 2209
BslFI GGGAC 2 cut(s) 927, 1110
BslI CCNNNNNNNGG 8 cut(s) 279, 442, 768, 898, 1115, 1116, 1162, 1380
BsmAI GTCTC 3 cut(s) 65, 923, 1293
BsmBI CGTCTC 2 cut(s) 923, 1293
BsmFI GGGAC 2 cut(s) 927, 1110
BsmI GAATGC 1 cut(s) 1700
BsnI GGCC 6 cut(s) 986, 1019, 1141, 1214, 1985, 2104
BsoBI CYCGRG 2 cut(s) 193, 206
Bsp1286I GDGCHC 5 cut(s) 205, 338, 1540, 2196, 2293
Bsp13I TCCGGA 1 cut(s) 338
BspANI GGCC 6 cut(s) 986, 1019, 1141, 1214, 1985, 2104
BspCNI CTCAG 5 cut(s) 292, 928, 988, 1056, 1141
BspEI TCCGGA 1 cut(s) 338
BspFNI CGCG 3 cut(s) 352, 989, 1130
BspHI TCATGA 1 cut(s) 1362
BspLI GGNNCC 6 cut(s) 127, 306, 337, 943, 1255, 1385
BspPI GGATC 4 cut(s) 1248, 1261, 2037, 2039
BspQI GCTCTTC 1 cut(s) 1528
BsrBI CCGCTC 1 cut(s) 1496
BsrFI RCCGGY 2 cut(s) 259, 2208
BsrI ACTGG 3 cut(s) 503, 2039, 2286
BssAI RCCGGY 2 cut(s) 259, 2208
BssECI CCNNGG 9 cut(s) 104, 193, 350, 987, 1020, 1061, 1128, 1379, 1896
BssNAI GTATAC 1 cut(s) 667
BssNI GRCGYC 1 cut(s) 2266
BssSI CACGAG 1 cut(s) 1035
BssT1I CCWWGG 2 cut(s) 104, 1896
Bst1107I GTATAC 1 cut(s) 667
Bst2BI CACGAG 1 cut(s) 1035
Bst2UI CCWGG 6 cut(s) 250, 280, 1216, 1351, 1380, 1400
Bst4CI ACNGT 5 cut(s) 309, 671, 1840, 1883, 1933
Bst6I CTCTTC 5 cut(s) 969, 1115, 1528, 1812, 2163
BstACI GRCGYC 1 cut(s) 2266
BstC8I GCNNGC 3 cut(s) 334, 1698, 2301
BstDEI CTNAG 5 cut(s) 300, 915, 996, 1043, 1149
BstDSI CCRYGG 3 cut(s) 350, 987, 1128
BstF5I GGATG 4 cut(s) 1236, 1762, 1801, 2071
BstFNI CGCG 3 cut(s) 352, 989, 1130
BstH2I RGCGCY 1 cut(s) 730
BstHHI GCGC 1 cut(s) 729
BstMAI GTCTC 3 cut(s) 65, 923, 1293
BstMCI CGRYCG 1 cut(s) 892
BstMWI GCNNNNNNNGC 5 cut(s) 992, 1025, 1104, 1343, 1693
BstNI CCWGG 6 cut(s) 250, 280, 1216, 1351, 1380, 1400
BstNSI RCATGY 1 cut(s) 1722
BstPAI GACNNNNGTC 1 cut(s) 2265
BstSFI CTRYAG 1 cut(s) 1836
BstSLI GKGCMC 1 cut(s) 2293
BstUI CGCG 3 cut(s) 352, 989, 1130
BstV1I GCAGC 8 cut(s) 17, 110, 234, 463, 816, 1004, 1358, 1475
BstV2I GAAGAC 6 cut(s) 402, 764, 842, 1299, 1448, 1496
BstX2I RGATCY 3 cut(s) 169, 680, 1253
BstXI CCANNNNNNTGG 1 cut(s) 796
BstYI RGATCY 3 cut(s) 169, 680, 1253
BstZ17I GTATAC 1 cut(s) 667
Bsu36I CCTNAGG 1 cut(s) 300
BsuRI GGCC 6 cut(s) 986, 1019, 1141, 1214, 1985, 2104
BtgI CCRYGG 3 cut(s) 350, 987, 1128
BtsCI GGATG 4 cut(s) 1236, 1762, 1801, 2071
BtsIMutI CAGTG 4 cut(s) 455, 1888, 2032, 2293
Cac8I GCNNGC 3 cut(s) 334, 1698, 2301
CaiI CAGNNNCTG 1 cut(s) 74
CciI TCATGA 1 cut(s) 1362
CfoI GCGC 1 cut(s) 729
Cfr10I RCCGGY 2 cut(s) 259, 2208
Cfr13I GGNCC 1 cut(s) 941
Cfr42I CCGCGG 3 cut(s) 353, 990, 1131
Cfr9I CCCGGG 1 cut(s) 193
CseI GACGC 3 cut(s) 374, 940, 1453
Csp6I GTAC 1 cut(s) 2206
CspAI ACCGGT 1 cut(s) 2208
CviAII CATG 7 cut(s) 872, 1363, 1475, 1652, 1719, 2039, 2242
CviQI GTAC 1 cut(s) 2206
DdeI CTNAG 5 cut(s) 300, 915, 996, 1043, 1149
DraI TTTAAA 1 cut(s) 1525
DrdI GACNNNNNNGTC 1 cut(s) 2231
DseDI GACNNNNNNGTC 1 cut(s) 2231
EaeI YGGCCR 2 cut(s) 1983, 2102
Eam1104I CTCTTC 5 cut(s) 969, 1115, 1528, 1812, 2163
EarI CTCTTC 5 cut(s) 969, 1115, 1528, 1812, 2163
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 2 cut(s) 203, 2194
Eco130I CCWWGG 2 cut(s) 104, 1896
Eco24I GRGCYC 3 cut(s) 205, 338, 2196
Eco32I GATATC 1 cut(s) 2094
Eco47I GGWCC 1 cut(s) 941
Eco53kI GAGCTC 2 cut(s) 203, 2194
Eco57I CTGAAG 2 cut(s) 854, 1868
Eco81I CCTNAGG 1 cut(s) 300
Eco88I CYCGRG 2 cut(s) 193, 206
EcoICRI GAGCTC 2 cut(s) 203, 2194
EcoRI GAATTC 1 cut(s) 600
EcoRII CCWGG 6 cut(s) 248, 278, 1214, 1349, 1378, 1398
EcoRV GATATC 1 cut(s) 2094
EcoT14I CCWWGG 2 cut(s) 104, 1896
EcoT22I ATGCAT 1 cut(s) 1522
EcoT38I GRGCYC 3 cut(s) 205, 338, 2196
ErhI CCWWGG 2 cut(s) 104, 1896
Esp3I CGTCTC 2 cut(s) 923, 1293
FaeI CATG 7 cut(s) 875, 1366, 1478, 1655, 1722, 2042, 2245
FaqI GGGAC 2 cut(s) 927, 1110
FatI CATG 7 cut(s) 871, 1362, 1474, 1651, 1718, 2038, 2241
FauI CCCGC 2 cut(s) 325, 1135
FbaI TGATCA 2 cut(s) 1177, 2175
FblI GTMKAC 2 cut(s) 87, 666
FokI GGATG 4 cut(s) 1243, 1749, 1808, 2078
FriOI GRGCYC 3 cut(s) 205, 338, 2196
FspBI CTAG 3 cut(s) 1137, 1268, 1541
GlaI GCGC 1 cut(s) 728
GsaI CCCAGC 1 cut(s) 1687
GsuI CTGGAG 3 cut(s) 172, 1237, 1421
HaeII RGCGCY 1 cut(s) 730
HaeIII GGCC 6 cut(s) 986, 1019, 1141, 1214, 1985, 2104
HapII CCGG 8 cut(s) 176, 194, 260, 339, 892, 1020, 1172, 2209
HgaI GACGC 3 cut(s) 374, 940, 1453
HhaI GCGC 1 cut(s) 729
Hin1I GRCGYC 1 cut(s) 2266
Hin1II CATG 7 cut(s) 875, 1366, 1478, 1655, 1722, 2042, 2245
Hin6I GCGC 1 cut(s) 727
HinP1I GCGC 1 cut(s) 727
HincII GTYRAC 2 cut(s) 88, 877
HindII GTYRAC 2 cut(s) 88, 877
HindIII AAGCTT 1 cut(s) 1434
HinfI GANTC 8 cut(s) 146, 457, 499, 529, 895, 1123, 1304, 1800
HpaII CCGG 8 cut(s) 176, 194, 260, 339, 892, 1020, 1172, 2209
HphI GGTGA 5 cut(s) 56, 274, 466, 619, 1790
Hpy166II GTNNAC 7 cut(s) 88, 667, 877, 912, 1237, 1472, 1576
Hpy8I GTNNAC 7 cut(s) 88, 667, 877, 912, 1237, 1472, 1576
Hpy99I CGWCG 4 cut(s) 41, 92, 368, 389
HpyAV CCTTC 7 cut(s) 8, 739, 1009, 1676, 1972, 2020, 2280
HpyCH4III ACNGT 5 cut(s) 309, 671, 1840, 1883, 1933
HpyCH4IV ACGT 2 cut(s) 2232, 2266
HpyCH4V TGCA 7 cut(s) 77, 222, 489, 1186, 1463, 1520, 1700
HpyF10VI GCNNNNNNNGC 5 cut(s) 992, 1025, 1104, 1343, 1693
HpyF3I CTNAG 5 cut(s) 300, 915, 996, 1043, 1149
HpySE526I ACGT 2 cut(s) 2232, 2266
Hsp92I GRCGYC 1 cut(s) 2266
Hsp92II CATG 7 cut(s) 875, 1366, 1478, 1655, 1722, 2042, 2245
HspAI GCGC 1 cut(s) 727
Kpn2I TCCGGA 1 cut(s) 338
Ksp22I TGATCA 2 cut(s) 1177, 2175
KspI CCGCGG 3 cut(s) 353, 990, 1131
LguI GCTCTTC 1 cut(s) 1528
LmnI GCTCC 8 cut(s) 106, 131, 179, 200, 208, 251, 341, 1685
Lsp1109I GCAGC 8 cut(s) 17, 110, 234, 463, 816, 1004, 1358, 1475
LweI GCATC 5 cut(s) 37, 209, 421, 1401, 1771
MaeI CTAG 3 cut(s) 1137, 1268, 1541
MaeII ACGT 2 cut(s) 2232, 2266
MaeIII GTNAC 3 cut(s) 784, 2056, 2211
MbiI CCGCTC 1 cut(s) 1496
MflI RGATCY 3 cut(s) 169, 680, 1253
MhlI GDGCHC 5 cut(s) 205, 338, 1540, 2196, 2293
MlsI TGGCCA 1 cut(s) 1985
MluNI TGGCCA 1 cut(s) 1985
MlyI GAGTC 4 cut(s) 466, 1132, 1313, 1809
MmeI TCCRAC 2 cut(s) 178, 2283
Mox20I TGGCCA 1 cut(s) 1985
Mph1103I ATGCAT 1 cut(s) 1522
MroI TCCGGA 1 cut(s) 338
MroXI GAANNNNTTC 2 cut(s) 399, 1358
MscI TGGCCA 1 cut(s) 1985
MseI TTAA 9 cut(s) 674, 849, 1259, 1500, 1524, 1673, 1832, 2252, 2308
MslI CAYNNNNRTG 1 cut(s) 2037
Msp20I TGGCCA 1 cut(s) 1985
MspA1I CMGCKG 6 cut(s) 352, 538, 989, 995, 1130, 1571
MspI CCGG 8 cut(s) 176, 194, 260, 339, 892, 1020, 1172, 2209
Mva1269I GAATGC 1 cut(s) 1700
MvaI CCWGG 6 cut(s) 250, 280, 1216, 1351, 1380, 1400
MvnI CGCG 3 cut(s) 352, 989, 1130
MwoI GCNNNNNNNGC 5 cut(s) 992, 1025, 1104, 1343, 1693
NciI CCSGG 5 cut(s) 177, 194, 195, 1021, 1172
NlaIII CATG 7 cut(s) 875, 1366, 1478, 1655, 1722, 2042, 2245
NlaIV GGNNCC 6 cut(s) 127, 306, 337, 943, 1255, 1385
NmuCI GTSAC 2 cut(s) 784, 2056
NsiI ATGCAT 1 cut(s) 1522
NspI RCATGY 1 cut(s) 1722
PagI TCATGA 1 cut(s) 1362
PciI ACATGT 1 cut(s) 1718
PciSI GCTCTTC 1 cut(s) 1528
PcsI WCGNNNNNNNCGW 4 cut(s) 384, 390, 885, 2102
PctI GAATGC 1 cut(s) 1700
PdmI GAANNNNTTC 2 cut(s) 399, 1358
PfeI GAWTC 4 cut(s) 146, 499, 529, 895
PflFI GACNNNGTC 1 cut(s) 274
PfoI TCCNGGA 1 cut(s) 248
PinAI ACCGGT 1 cut(s) 2208
PleI GAGTC 4 cut(s) 465, 1131, 1312, 1808
PpsI GAGTC 4 cut(s) 465, 1131, 1312, 1808
PscI ACATGT 1 cut(s) 1718
PshAI GACNNNNGTC 1 cut(s) 2265
PsiI TTATAA 1 cut(s) 1595
Psp124BI GAGCTC 2 cut(s) 205, 2196
Psp6I CCWGG 6 cut(s) 248, 278, 1214, 1349, 1378, 1398
PspFI CCCAGC 1 cut(s) 1683
PspGI CCWGG 6 cut(s) 248, 278, 1214, 1349, 1378, 1398
PspN4I GGNNCC 6 cut(s) 127, 306, 337, 943, 1255, 1385
PspPI GGNCC 1 cut(s) 941
PstNI CAGNNNCTG 1 cut(s) 74
PsuI RGATCY 3 cut(s) 169, 680, 1253
PsyI GACNNNGTC 1 cut(s) 274
PvuII CAGCTG 2 cut(s) 995, 1571
RsaI GTAC 1 cut(s) 2207
RsaNI GTAC 1 cut(s) 2206
RseI CAYNNNNRTG 1 cut(s) 2037
SacI GAGCTC 2 cut(s) 205, 2196
SacII CCGCGG 3 cut(s) 353, 990, 1131
SalI GTCGAC 1 cut(s) 86
SapI GCTCTTC 1 cut(s) 1528
SaqAI TTAA 9 cut(s) 674, 849, 1259, 1500, 1524, 1673, 1832, 2252, 2308
Sau96I GGNCC 1 cut(s) 941
SchI GAGTC 4 cut(s) 466, 1132, 1313, 1809
SduI GDGCHC 5 cut(s) 205, 338, 1540, 2196, 2293
SfaNI GCATC 5 cut(s) 37, 209, 421, 1401, 1771
SfcI CTRYAG 1 cut(s) 1836
Sfr303I CCGCGG 3 cut(s) 353, 990, 1131
SgrAI CRCCGGYG 1 cut(s) 259
SgrBI CCGCGG 3 cut(s) 353, 990, 1131
SinI GGWCC 1 cut(s) 941
SmaI CCCGGG 1 cut(s) 195
SmiMI CAYNNNNRTG 1 cut(s) 2037
SrfI GCCCGGGC 1 cut(s) 195
SspMI CTAG 3 cut(s) 1137, 1268, 1541
SstI GAGCTC 2 cut(s) 205, 2196
StyI CCWWGG 2 cut(s) 104, 1896
TaaI ACNGT 5 cut(s) 309, 671, 1840, 1883, 1933
TaiI ACGT 2 cut(s) 2235, 2269
TaqI TCGA 9 cut(s) 36, 87, 288, 378, 393, 429, 888, 2166, 2196
TauI GCSGC 2 cut(s) 989, 992
TfiI GAWTC 4 cut(s) 146, 499, 529, 895
Tru1I TTAA 9 cut(s) 674, 849, 1259, 1500, 1524, 1673, 1832, 2252, 2308
Tru9I TTAA 9 cut(s) 674, 849, 1259, 1500, 1524, 1673, 1832, 2252, 2308
TscAI CASTG 4 cut(s) 455, 1888, 2039, 2293
TseFI GTSAC 2 cut(s) 784, 2056
TseI GCWGC 8 cut(s) 5, 98, 222, 476, 829, 992, 1346, 1463
Tsp45I GTSAC 2 cut(s) 784, 2056
TspGWI ACGGA 5 cut(s) 105, 172, 1266, 1755, 2037
TspMI CCCGGG 1 cut(s) 193
TspRI CASTG 4 cut(s) 455, 1888, 2039, 2293
Tth111I GACNNNGTC 1 cut(s) 274
VpaK11BI GGWCC 1 cut(s) 941
XapI RAATTY 4 cut(s) 552, 600, 631, 1937
XbaI TCTAGA 1 cut(s) 1267
XceI RCATGY 1 cut(s) 1722
XmaI CCCGGG 1 cut(s) 193
XmiI GTMKAC 2 cut(s) 87, 666
XmnI GAANNNNTTC 2 cut(s) 399, 1358
XspI CTAG 3 cut(s) 1137, 1268, 1541
ZraI GACGTC 1 cut(s) 2267
Zsp2I ATGCAT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.