Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Forward (+)

77169955 .. 77173608

Gene Structure 3654 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000036184

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2814 bp

ATGGATTTTTCATTGAAGAAGTCATTCAAACCCCATGGGTCGAGCAAACACATGAGAAAGATCTCAGCCGGAGCAGTAGAAGATAACAGCCACGAGGAGCTCCCGATTCTAATTGATCACCAGAACCAGGCAAGCATCGCCTCAGATTCGCCGGGCTACCACCGCCGAGAGGTCATCGTCAAGATCGACGAGGGCGATTCCTCCGCAACAACCATCAGAGAAAATGTCAGGGAAGTGCAGCATGGCGGGACGATTTGGCGGGAATCGAGTTTAGATTTTTGGACGGAGGACGACGGGAGCACTATCCCTGCCGATGGAAGCGCCGGCAGCGGGGGCTTTCATTTCGTGCAGAGGGGGAAGCAGACTGCGCCGGCGGCGGAGGAGGATCCGCCGTCGAAGCTTATAGGGCAGTACCTTAAGAAGCAGAGAGCCTCGGGCGGCGACGTGTCGTTGGACATGGATTTGGAGATGGACGAGCTACAAAAACCGCCGCTGGCGGAGCCTCCGAGGATCAGCGAGGCGTCGCGGGAGCTCAAGGTGTCGTTCCAGTCGCCGGCACGGTCGGAGCTCCCCGAGGGGGCGAACGATTCAGTTCGGAGGCGAAACAAGGACTCGGTGGTTGACGACGAACGACGTCGTAGCAGTCGGTTGAGTAACGGCGGAGCCGATGTGATTAGGTGCTCGTCAAACAAATCGTTTCGGCGAGAGGCTTCGTTTCAGAACAACAATTTGTTGAGGCTAAAGACCAAGTCTAGATTGATAGACCCGCCTGAAAGCTGGGACGAGGTTCGGGTCCCGAAATCGGGTCCGATGAAGTCCGGGATGTTGGGGAAAGGCGGCGGCGGTGACGATGACGACGAGGACCCGTTTTTGGAAGAGGATCTGCCTGATGAGTACAAGAGGGTAAAATTAGATGCATTGACTCTGCTCCAATGGCTTAGTTTGGTTTTGATCATTGGTGCCTTGGTTTGCACGCTTTCTCTAAGAGTTTTGAGGCAGAAGCATCTGTGGAAATTGAAGCTGTGGAAATGGGAGGTGCTGATTTTGGTTTCGATTTGCGGGAGGCTGGTTTCGGGTTGGGTGATAAAGATCATGGTGTTCTTCGTGGAAAGGAATTTCCTTCTGCGCAAGAGGGTGTTGTATTTTGTTTATGGGATCAGAAGGGCTGTGCAGAACTGTATATGGCTTGGCTTTGTTTTGACGGCTTGGCATTTCATGCTAATCAGGAAGGTTGAGCTGGAAACCAATAGCAAAATACTAGAGTATGTGACGAAAGGTTTGCTTTGTCTCTTGATCGGGGTTTTGCTATGGTTGTTAAAGACCCTGGTTGTGAAGGTGCTTGCTTCCTCTTTCCACGTTAGATCCTACTTTGATCGGATTCAGGACGCGCTTTTTAATCAGTATGTGATCCAAACTCTCTCTGGGCGTCCCTTGATTGAGGTACAGAATGCGGAGGATGAAGAAGAGAGGCTTGCTGAGGAGGTTAGGAAGTTACAGAATGCTGGGGCAACTATGCCTCCTGACCTCAGATCAAATGCATTCCCTTCTGCGAGAATTGGGAGGGCGGTTGGGAGTGGCTCTGGGAGAAGTGGGAGGTTCATTGCTACGAGTGGGGGGCTTGGGGGAAAAAGTGGCAAGTTTTCAAGGCCAACAATGTCCAAGAAGTCAGAGGAGGCTGCAATTACCATTGATCATTTGCATAGGCTCAATCCAAAGAATGTATCTGCTTGGAATATGAAGAGGCTAATGAACATTGTTAGAAAAGGGCATCTTACGACACTCGATGAGCAAATTCTGGATTCCAGCGCTCAGGATGAGGGTGATACTATGATCAAAAGTGAAGTTGAGGCAAAGGCTGCAGCTAAAAAGATATTTCGCAATGTGGCACGGCCTGGCTCAAAGCACATCTACCTGGAGGATTTGTTGCGTTTCATGGGAGAAGAGGAGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCCGAGACTCGCAGAATCCGCAAAAATTCCTTGAAAAATTGGGTGGTCAACTGCTTTAGAGAACGGAGAGCACTTTCTTTGACACTGAATGACACCAAAACAGCGGTGAACAGACTTCGACACGTGGTGGATGTAATAGTTGGCATTGTTATTATAGTTATATGGCTTCTTGTGCTGGATATTGTCACCAGCGCCAATCTTGTTTATGCAACTTCTCAGCTTGTGGTCGTGGCATTTGTTTTTGGTAACACTTGCAGGACAGTATTTGAAGCAGTCATATTTTTATTTGTTATGCACCCATTTGACGTGGGAGATCGATGTGAAATTCAAGGGGTACAGATGGTTGTAGAAGAAATGAATATCTTGACTACAGTTTTTCTAAGGTATGACAACATAAAAATCGTGTACCCGAACAGTACACTTTCAACTATGCCCATCAATAACTTCTATCGTAGTCCGGACACAGGAGATGTTGTTGAGTTCTGTGTCCATATATCTACTCCACAAGATAAGATTGCTGTTATGAAGCAGAGAATGATAAGTTACATTGAAAGTAAAAAGGAGCACTGGTATCCTCAAGTACAGATCGTATTGAAGGATGTAGAAGAATTAAATAAGATGAGGTATGCATTGTGGCTGACACATAAGATGAACTTCCAAGATGTGGGAGAAAAGTGGGAAAGAAGAGCCCTTTTACTTGAAGAGATGGTAAGGATCTTCCAGGAGCTGGACATTCAGTATCGTTTGCTGCCTATTGACATAAATGTCCGCGCCATGCCTCCCACAACCACCACCGGTCTGCGTCCGTCAAATTTTTCAGCAACTACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

938

Amino Acids

106.22

Weight (kDa)

8.93

Isoelectric Point (pI)

47.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	753 - 809	6.8e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2744
AatII	GACGTC	1 cut(s)	637
Acc16I	TGCGCA	1 cut(s)	1127
AccB1I	GGYRCC	1 cut(s)	959
AccB7I	CCANNNNNTGG	2 cut(s)	2644, 2707
AccII	CGCG	3 cut(s)	526, 1388, 2751
AccIII	TCCGGA	1 cut(s)	2437
AclWI	GGATC	8 cut(s)	380, 393, 518, 888, 1163, 1356, 1402, 2702
AcsI	RAATTY	5 cut(s)	1114, 1791, 2005, 2304, 2791
AcvI	CACGTG	1 cut(s)	2104
AcyI	GRCGYC	3 cut(s)	521, 634, 1426
AdeI	CACNNNGTG	1 cut(s)	2107
AfaI	GTAC	7 cut(s)	413, 896, 1443, 2316, 2387, 2398, 2562
AfeI	AGCGCT	1 cut(s)	1807
AflII	CTTAAG	1 cut(s)	416
AflIII	ACRYGT	2 cut(s)	444, 2101
AgeI	ACCGGT	1 cut(s)	2774
AhdI	GACNNNNNGTC	1 cut(s)	1962
AjiI	CACGTC	2 cut(s)	445, 2287
AjnI	CCWGG	5 cut(s)	126, 1323, 1891, 1911, 2700
Alw21I	GWGCWC	7 cut(s)	102, 302, 534, 570, 683, 2053, 2547
Alw26I	GTCTC	3 cut(s)	1292, 1970, 1979
AlwI	GGATC	8 cut(s)	380, 393, 518, 888, 1163, 1356, 1402, 2702
AlwNI	CAGNNNCTG	1 cut(s)	2707
Ama87I	CYCGRG	2 cut(s)	433, 572
Aor13HI	TCCGGA	1 cut(s)	2437
Aor51HI	AGCGCT	1 cut(s)	1807
AoxI	GGCC	3 cut(s)	1646, 1889, 1977
ApeKI	GCWGC	6 cut(s)	238, 327, 1676, 1856, 1859, 2728
ApoI	RAATTY	5 cut(s)	1114, 1791, 2005, 2304, 2791
ArsI	GACNNNNNNTTYG	2 cut(s)	1261, 1293
AsiGI	ACCGGT	1 cut(s)	2774
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	7 cut(s)	323, 370, 1128, 1390, 1808, 2174, 2753
AspS9I	GGNCC	4 cut(s)	793, 806, 862, 1977
AsuC2I	CCSGG	2 cut(s)	153, 820
AsuHPI	GGTGA	6 cut(s)	110, 857, 1093, 1832, 2098, 2158
AvaI	CYCGRG	2 cut(s)	433, 572
AvaII	GGWCC	3 cut(s)	793, 806, 862
BamHI	GGATCC	1 cut(s)	385
BanI	GGYRCC	1 cut(s)	959
BanII	GRGCYC	4 cut(s)	102, 534, 570, 2671
BauI	CACGAG	1 cut(s)	92
BbrPI	CACGTG	1 cut(s)	2104
Bbv12I	GWGCWC	7 cut(s)	102, 302, 534, 570, 683, 2053, 2547
BbvCI	CCTCAGC	1 cut(s)	1476
BbvI	GCAGC	6 cut(s)	250, 339, 1663, 1843, 1871, 2715
BccI	CCATC	6 cut(s)	221, 308, 463, 2314, 2423, 2680
BceAI	ACGGC	4 cut(s)	376, 673, 1218, 1904
BcgI	CGANNNNNNTGC	2 cut(s)	1261, 1295
BciT130I	CCWGG	5 cut(s)	128, 1325, 1893, 1913, 2702
BciVI	GTATCC	1 cut(s)	2562
BclI	TGATCA	4 cut(s)	115, 951, 1690, 1830
BcnI	CCSGG	2 cut(s)	153, 820
BcoDI	GTCTC	3 cut(s)	1292, 1970, 1979
BfaI	CTAG	2 cut(s)	753, 1259
BfmI	CTRYAG	2 cut(s)	1857, 2349
BfoI	RGCGCY	3 cut(s)	324, 1809, 2175
BfrI	CTTAAG	1 cut(s)	416
BfuI	GTATCC	1 cut(s)	2562
BglII	AGATCT	1 cut(s)	60
Bme1390I	CCNGG	7 cut(s)	128, 153, 820, 1325, 1893, 1913, 2702
Bme18I	GGWCC	3 cut(s)	793, 806, 862
BmeRI	GACNNNNNGTC	1 cut(s)	1962
BmeT110I	CYCGRG	2 cut(s)	433, 572
BmgBI	CACGTC	2 cut(s)	445, 2287
BmgT120I	GGNCC	4 cut(s)	793, 806, 862, 1977
BmiI	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 864, 961, 1978
BmrFI	CCNGG	7 cut(s)	128, 153, 820, 1325, 1893, 1913, 2702
BmsI	GCATC	4 cut(s)	144, 904, 1012, 1777
BpmI	CTGGAG	1 cut(s)	1934
Bpu10I	CCTNAGC	2 cut(s)	1476, 1809
BpuEI	CTTGAG	2 cut(s)	518, 2541
BpuMI	CCSGG	2 cut(s)	153, 820
Bsa29I	ATCGAT	1 cut(s)	2296
BsaAI	YACGTR	1 cut(s)	2104
BsaBI	GATNNNNATC	1 cut(s)	1088
BsaHI	GRCGYC	3 cut(s)	521, 634, 1426
BsaJI	CCNNGG	6 cut(s)	34, 432, 506, 573, 963, 1323
BsaWI	WCCGGW	2 cut(s)	2437, 2774
Bse118I	RCCGGY	4 cut(s)	323, 370, 553, 2774
Bse1I	ACTGG	2 cut(s)	547, 2552
Bse3DI	GCAATG	2 cut(s)	1599, 1885
Bse8I	GATNNNNATC	1 cut(s)	1088
BseAI	TCCGGA	1 cut(s)	2437
BseBI	CCWGG	5 cut(s)	128, 1325, 1893, 1913, 2702
BseCI	ATCGAT	1 cut(s)	2296
BseDI	CCNNGG	6 cut(s)	34, 432, 506, 573, 963, 1323
BseGI	GGATG	5 cut(s)	828, 1462, 1819, 2116, 2584
BseJI	GATNNNNATC	1 cut(s)	1088
BseMI	GCAATG	2 cut(s)	1599, 1885
BseMII	CTCAG	6 cut(s)	78, 156, 1467, 1540, 1823, 2210
BseNI	ACTGG	2 cut(s)	547, 2552
BseRI	GAGGAG	5 cut(s)	110, 395, 1493, 1685, 1958
BseXI	GCAGC	6 cut(s)	250, 339, 1663, 1843, 1871, 2715
BseYI	CCCAGC	2 cut(s)	777, 1502
BsgI	GTGCAG	3 cut(s)	257, 368, 1190
Bsh1236I	CGCG	3 cut(s)	526, 1388, 2751
Bsh1285I	CGRYCG	1 cut(s)	563
BshFI	GGCC	3 cut(s)	1648, 1891, 1979
BshNI	GGYRCC	1 cut(s)	959
BshTI	ACCGGT	1 cut(s)	2774
BshVI	ATCGAT	1 cut(s)	2296
BsiEI	CGRYCG	1 cut(s)	563
BsiHKAI	GWGCWC	7 cut(s)	102, 302, 534, 570, 683, 2053, 2547
BsiHKCI	CYCGRG	2 cut(s)	433, 572
BsiSI	CCGG	8 cut(s)	69, 152, 324, 371, 554, 819, 2438, 2775
BslFI	GGGAC	4 cut(s)	262, 779, 794, 1413
BsmAI	GTCTC	3 cut(s)	1292, 1970, 1979
BsmFI	GGGAC	4 cut(s)	262, 779, 794, 1413
BsmI	GAATGC	3 cut(s)	1453, 1504, 1538
BsnI	GGCC	3 cut(s)	1648, 1891, 1979
BsoBI	CYCGRG	2 cut(s)	433, 572
Bsp1286I	GDGCHC	8 cut(s)	102, 302, 534, 570, 683, 2053, 2547, 2671
Bsp13I	TCCGGA	1 cut(s)	2437
Bsp19I	CCATGG	1 cut(s)	34
BspANI	GGCC	3 cut(s)	1648, 1891, 1979
BspCNI	CTCAG	6 cut(s)	77, 155, 1468, 1539, 1822, 2209
BspDI	ATCGAT	1 cut(s)	2296
BspEI	TCCGGA	1 cut(s)	2437
BspFNI	CGCG	3 cut(s)	526, 1388, 2751
BspLI	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 864, 961, 1978
BspMAI	CTGCAG	1 cut(s)	1861
BspPI	GGATC	8 cut(s)	380, 393, 518, 888, 1163, 1356, 1402, 2702
BspQI	GCTCTTC	1 cut(s)	2659
BspT107I	GGYRCC	1 cut(s)	959
BspTI	CTTAAG	1 cut(s)	416
BsrDI	GCAATG	2 cut(s)	1599, 1885
BsrFI	RCCGGY	4 cut(s)	323, 370, 553, 2774
BsrI	ACTGG	2 cut(s)	547, 2552
BssAI	RCCGGY	4 cut(s)	323, 370, 553, 2774
BssECI	CCNNGG	6 cut(s)	34, 432, 506, 573, 963, 1323
BssNI	GRCGYC	3 cut(s)	521, 634, 1426
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	2 cut(s)	34, 963
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	5 cut(s)	128, 1325, 1893, 1913, 2702
Bst4CI	ACNGT	5 cut(s)	561, 1178, 2242, 2353, 2396
Bst6I	CTCTTC	6 cut(s)	870, 1458, 1733, 1935, 2659, 2676
BstACI	GRCGYC	3 cut(s)	521, 634, 1426
BstAFI	CTTAAG	1 cut(s)	416
BstAPI	GCANNNNNTGC	2 cut(s)	1216, 1856
BstBAI	YACGTR	1 cut(s)	2104
BstC8I	GCNNGC	8 cut(s)	133, 325, 372, 495, 555, 974, 1341, 1473
BstDEI	CTNAG	9 cut(s)	64, 142, 938, 983, 1476, 1526, 1809, 2196, 2360
BstDSI	CCRYGG	1 cut(s)	34
BstF5I	GGATG	5 cut(s)	828, 1462, 1819, 2116, 2584
BstFNI	CGCG	3 cut(s)	526, 1388, 2751
BstH2I	RGCGCY	3 cut(s)	324, 1809, 2175
BstHHI	GCGC	7 cut(s)	323, 370, 1128, 1390, 1808, 2174, 2753
BstMAI	GTCTC	3 cut(s)	1292, 1970, 1979
BstMCI	CGRYCG	1 cut(s)	563
BstNI	CCWGG	5 cut(s)	128, 1325, 1893, 1913, 2702
BstSCI	CCNGG	7 cut(s)	126, 151, 818, 1323, 1891, 1911, 2700
BstSFI	CTRYAG	2 cut(s)	1857, 2349
BstUI	CGCG	3 cut(s)	526, 1388, 2751
BstV1I	GCAGC	6 cut(s)	250, 339, 1663, 1843, 1871, 2715
BstX2I	RGATCY	5 cut(s)	60, 385, 880, 1361, 2694
BstYI	RGATCY	5 cut(s)	60, 385, 880, 1361, 2694
Bsu15I	ATCGAT	1 cut(s)	2296
BsuI	GTATCC	1 cut(s)	2562
BsuRI	GGCC	3 cut(s)	1648, 1891, 1979
BsuTUI	ATCGAT	1 cut(s)	2296
BtgI	CCRYGG	1 cut(s)	34
BtgZI	GCGATG	1 cut(s)	121
BtrI	CACGTC	2 cut(s)	445, 2287
BtsCI	GGATG	5 cut(s)	828, 1462, 1819, 2116, 2584
BtsIMutI	CAGTG	2 cut(s)	2063, 2545
Cac8I	GCNNGC	8 cut(s)	133, 325, 372, 495, 555, 974, 1341, 1473
CaiI	CAGNNNCTG	1 cut(s)	2707
CfoI	GCGC	7 cut(s)	323, 370, 1128, 1390, 1808, 2174, 2753
Cfr10I	RCCGGY	4 cut(s)	323, 370, 553, 2774
Cfr13I	GGNCC	4 cut(s)	793, 806, 862, 1977
ClaI	ATCGAT	1 cut(s)	2296
CseI	GACGC	4 cut(s)	510, 1394, 1415, 2771
Csp6I	GTAC	7 cut(s)	412, 895, 1442, 2315, 2386, 2397, 2561
CspAI	ACCGGT	1 cut(s)	2774
CviAII	CATG	9 cut(s)	35, 52, 242, 457, 1093, 1216, 1933, 1960, 2755
CviQI	GTAC	7 cut(s)	412, 895, 1442, 2315, 2386, 2397, 2561
DdeI	CTNAG	9 cut(s)	64, 142, 938, 983, 1476, 1526, 1809, 2196, 2360
DraI	TTTAAA	1 cut(s)	1953
DraIII	CACNNNGTG	1 cut(s)	2107
DrdI	GACNNNNNNGTC	1 cut(s)	2744
DriI	GACNNNNNGTC	1 cut(s)	1962
DseDI	GACNNNNNNGTC	1 cut(s)	2744
Eam1104I	CTCTTC	6 cut(s)	870, 1458, 1733, 1935, 2659, 2676
Eam1105I	GACNNNNNGTC	1 cut(s)	1962
EarI	CTCTTC	6 cut(s)	870, 1458, 1733, 1935, 2659, 2676
EciI	GGCGGA	4 cut(s)	378, 392, 512, 675
Ecl136II	GAGCTC	3 cut(s)	100, 532, 568
Eco130I	CCWWGG	2 cut(s)	34, 963
Eco24I	GRGCYC	4 cut(s)	102, 534, 570, 2671
Eco47I	GGWCC	3 cut(s)	793, 806, 862
Eco47III	AGCGCT	1 cut(s)	1807
Eco53kI	GAGCTC	3 cut(s)	100, 532, 568
Eco72I	CACGTG	1 cut(s)	2104
Eco88I	CYCGRG	2 cut(s)	433, 572
EcoICRI	GAGCTC	3 cut(s)	100, 532, 568
EcoO109I	RGGNCCY	3 cut(s)	793, 862, 1977
EcoRII	CCWGG	5 cut(s)	126, 1323, 1891, 1911, 2700
EcoT14I	CCWWGG	2 cut(s)	34, 963
EcoT22I	ATGCAT	3 cut(s)	919, 1540, 2611
EcoT38I	GRGCYC	4 cut(s)	102, 534, 570, 2671
ErhI	CCWWGG	2 cut(s)	34, 963
FaeI	CATG	9 cut(s)	38, 55, 245, 460, 1096, 1219, 1936, 1963, 2758
FalI	AAGNNNNNCTT	8 cut(s)	1266, 1298, 1455, 1487, 1755, 1787, 2618, 2650
FaqI	GGGAC	4 cut(s)	262, 779, 794, 1413
FatI	CATG	9 cut(s)	34, 51, 241, 456, 1092, 1215, 1932, 1959, 2754
FauI	CCCGC	6 cut(s)	239, 252, 323, 519, 774, 1052
FbaI	TGATCA	4 cut(s)	115, 951, 1690, 1830
FokI	GGATG	5 cut(s)	835, 1469, 1826, 2123, 2591
FriOI	GRGCYC	4 cut(s)	102, 534, 570, 2671
FspBI	CTAG	2 cut(s)	753, 1259
FspI	TGCGCA	1 cut(s)	1127
GlaI	GCGC	7 cut(s)	322, 369, 1127, 1389, 1807, 2173, 2752
GsaI	CCCAGC	2 cut(s)	781, 1506
GsuI	CTGGAG	1 cut(s)	1934
HaeII	RGCGCY	3 cut(s)	324, 1809, 2175
HaeIII	GGCC	3 cut(s)	1648, 1891, 1979
HapII	CCGG	8 cut(s)	69, 152, 324, 371, 554, 819, 2438, 2775
HgaI	GACGC	4 cut(s)	510, 1394, 1415, 2771
HhaI	GCGC	7 cut(s)	323, 370, 1128, 1390, 1808, 2174, 2753
Hin1I	GRCGYC	3 cut(s)	521, 634, 1426
Hin1II	CATG	9 cut(s)	38, 55, 245, 460, 1096, 1219, 1936, 1963, 2758
Hin6I	GCGC	7 cut(s)	321, 368, 1126, 1388, 1806, 2172, 2751
HinP1I	GCGC	7 cut(s)	321, 368, 1126, 1388, 1806, 2172, 2751
HincII	GTYRAC	2 cut(s)	622, 2029
HindII	GTYRAC	2 cut(s)	622, 2029
HindIII	AAGCTT	1 cut(s)	398
HpaII	CCGG	8 cut(s)	69, 152, 324, 371, 554, 819, 2438, 2775
HphI	GGTGA	6 cut(s)	110, 857, 1093, 1832, 2098, 2158
Hpy166II	GTNNAC	5 cut(s)	622, 2029, 2089, 2386, 2399
Hpy8I	GTNNAC	5 cut(s)	622, 2029, 2089, 2386, 2399
Hpy99I	CGWCG	9 cut(s)	191, 296, 397, 446, 526, 629, 636, 639, 860
HpyAV	CCTTC	7 cut(s)	1130, 1155, 1222, 1327, 1554, 1967, 2569
HpyCH4III	ACNGT	5 cut(s)	561, 1178, 2242, 2353, 2396
HpyCH4IV	ACGT	5 cut(s)	444, 634, 1356, 2103, 2286
HpyF3I	CTNAG	9 cut(s)	64, 142, 938, 983, 1476, 1526, 1809, 2196, 2360
HpySE526I	ACGT	5 cut(s)	444, 634, 1356, 2103, 2286
Hsp92I	GRCGYC	3 cut(s)	521, 634, 1426
Hsp92II	CATG	9 cut(s)	38, 55, 245, 460, 1096, 1219, 1936, 1963, 2758
HspAI	GCGC	7 cut(s)	321, 368, 1126, 1388, 1806, 2172, 2751
KflI	GGGWCCC	1 cut(s)	793
Kpn2I	TCCGGA	1 cut(s)	2437
KroI	GCCGGC	3 cut(s)	323, 370, 553
KroNI	GCCGGC	3 cut(s)	325, 372, 555
Ksp22I	TGATCA	4 cut(s)	115, 951, 1690, 1830
LguI	GCTCTTC	1 cut(s)	2659
Lsp1109I	GCAGC	6 cut(s)	250, 339, 1663, 1843, 1871, 2715
LweI	GCATC	4 cut(s)	144, 904, 1012, 1777
MaeI	CTAG	2 cut(s)	753, 1259
MaeII	ACGT	5 cut(s)	444, 634, 1356, 2103, 2286
MaeIII	GTNAC	7 cut(s)	653, 845, 1267, 1491, 2164, 2225, 2522
MflI	RGATCY	5 cut(s)	60, 385, 880, 1361, 2694
MhlI	GDGCHC	8 cut(s)	102, 302, 534, 570, 683, 2053, 2547, 2671
MlyI	GAGTC	4 cut(s)	605, 916, 1972, 1981
MmeI	TCCRAC	2 cut(s)	432, 543
Mph1103I	ATGCAT	3 cut(s)	919, 1540, 2611
MreI	CGCCGGCG	1 cut(s)	370
MroI	TCCGGA	1 cut(s)	2437
MroNI	GCCGGC	3 cut(s)	323, 370, 553
MroXI	GAANNNNTTC	1 cut(s)	23
MseI	TTAA	5 cut(s)	417, 1316, 1395, 1952, 2591
MspA1I	CMGCKG	3 cut(s)	330, 493, 2084
MspCI	CTTAAG	1 cut(s)	416
MspI	CCGG	8 cut(s)	69, 152, 324, 371, 554, 819, 2438, 2775
MspR9I	CCNGG	7 cut(s)	128, 153, 820, 1325, 1893, 1913, 2702
Mva1269I	GAATGC	3 cut(s)	1453, 1504, 1538
MvaI	CCWGG	5 cut(s)	128, 1325, 1893, 1913, 2702
MvnI	CGCG	3 cut(s)	526, 1388, 2751
NaeI	GCCGGC	3 cut(s)	325, 372, 555
NciI	CCSGG	2 cut(s)	153, 820
NcoI	CCATGG	1 cut(s)	34
NgoMIV	GCCGGC	3 cut(s)	323, 370, 553
NlaIII	CATG	9 cut(s)	38, 55, 245, 460, 1096, 1219, 1936, 1963, 2758
NlaIV	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 864, 961, 1978
NmeAIII	GCCGAG	1 cut(s)	191
NmuCI	GTSAC	3 cut(s)	845, 1267, 2164
NsbI	TGCGCA	1 cut(s)	1127
NsiI	ATGCAT	3 cut(s)	919, 1540, 2611
PciSI	GCTCTTC	1 cut(s)	2659
PcsI	WCGNNNNNNNCGW	4 cut(s)	183, 192, 663, 855
PctI	GAATGC	3 cut(s)	1453, 1504, 1538
PdiI	GCCGGC	3 cut(s)	325, 372, 555
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	8 cut(s)	106, 146, 197, 263, 587, 1378, 1799, 1995
PflFI	GACNNNGTC	1 cut(s)	748
PflMI	CCANNNNNTGG	2 cut(s)	2644, 2707
PfoI	TCCNGGA	2 cut(s)	818, 2700
PinAI	ACCGGT	1 cut(s)	2774
PleI	GAGTC	4 cut(s)	605, 916, 1971, 1981
PmaCI	CACGTG	1 cut(s)	2104
PmlI	CACGTG	1 cut(s)	2104
PpsI	GAGTC	4 cut(s)	605, 916, 1971, 1981
Ppu21I	YACGTR	1 cut(s)	2104
PpuMI	RGGWCCY	2 cut(s)	793, 862
Psp124BI	GAGCTC	3 cut(s)	102, 534, 570
Psp5II	RGGWCCY	2 cut(s)	793, 862
Psp6I	CCWGG	5 cut(s)	126, 1323, 1891, 1911, 2700
PspCI	CACGTG	1 cut(s)	2104
PspFI	CCCAGC	2 cut(s)	777, 1502
PspGI	CCWGG	5 cut(s)	126, 1323, 1891, 1911, 2700
PspN4I	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 864, 961, 1978
PspPI	GGNCC	4 cut(s)	793, 806, 862, 1977
PspPPI	RGGWCCY	2 cut(s)	793, 862
PstI	CTGCAG	1 cut(s)	1861
PstNI	CAGNNNCTG	1 cut(s)	2707
PsuI	RGATCY	5 cut(s)	60, 385, 880, 1361, 2694
PsyI	GACNNNGTC	1 cut(s)	748
RsaI	GTAC	7 cut(s)	413, 896, 1443, 2316, 2387, 2398, 2562
RsaNI	GTAC	7 cut(s)	412, 895, 1442, 2315, 2386, 2397, 2561
SacI	GAGCTC	3 cut(s)	102, 534, 570
SapI	GCTCTTC	1 cut(s)	2659
SaqAI	TTAA	5 cut(s)	417, 1316, 1395, 1952, 2591
Sau96I	GGNCC	4 cut(s)	793, 806, 862, 1977
SchI	GAGTC	4 cut(s)	605, 916, 1972, 1981
ScrFI	CCNGG	7 cut(s)	128, 153, 820, 1325, 1893, 1913, 2702
SduI	GDGCHC	8 cut(s)	102, 302, 534, 570, 683, 2053, 2547, 2671
SfaNI	GCATC	4 cut(s)	144, 904, 1012, 1777
SfcI	CTRYAG	2 cut(s)	1857, 2349
SgrAI	CRCCGGYG	1 cut(s)	370
SinI	GGWCC	3 cut(s)	793, 806, 862
SmlI	CTYRAG	3 cut(s)	416, 533, 2556
SmoI	CTYRAG	3 cut(s)	416, 533, 2556
SspMI	CTAG	2 cut(s)	753, 1259
SstI	GAGCTC	3 cut(s)	102, 534, 570
StyD4I	CCNGG	7 cut(s)	126, 151, 818, 1323, 1891, 1911, 2700
StyI	CCWWGG	2 cut(s)	34, 963
TaaI	ACNGT	5 cut(s)	561, 1178, 2242, 2353, 2396
TaiI	ACGT	5 cut(s)	447, 637, 1359, 2106, 2289
TaqI	TCGA	8 cut(s)	41, 186, 266, 395, 1052, 1782, 2098, 2296
TatI	WGTACW	3 cut(s)	894, 2396, 2560
TauI	GCSGC	5 cut(s)	377, 441, 493, 840, 843
TfiI	GAWTC	8 cut(s)	106, 146, 197, 263, 587, 1378, 1799, 1995
Tru1I	TTAA	5 cut(s)	417, 1316, 1395, 1952, 2591
Tru9I	TTAA	5 cut(s)	417, 1316, 1395, 1952, 2591
TscAI	CASTG	2 cut(s)	2070, 2552
TseFI	GTSAC	3 cut(s)	845, 1267, 2164
TseI	GCWGC	6 cut(s)	238, 327, 1676, 1856, 1859, 2728
Tsp45I	GTSAC	3 cut(s)	845, 1267, 2164
TspGWI	ACGGA	3 cut(s)	299, 2059, 2775
TspRI	CASTG	2 cut(s)	2070, 2552
Tth111I	GACNNNGTC	1 cut(s)	748
Van91I	CCANNNNNTGG	2 cut(s)	2644, 2707
Vha464I	CTTAAG	1 cut(s)	416
VpaK11BI	GGWCC	3 cut(s)	793, 806, 862
XapI	RAATTY	5 cut(s)	1114, 1791, 2005, 2304, 2791
XbaI	TCTAGA	1 cut(s)	752
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1418
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	2 cut(s)	753, 1259
ZraI	GACGTC	1 cut(s)	635
Zsp2I	ATGCAT	3 cut(s)	919, 1540, 2611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.