Rroxscaffold_7G00164430

Tyrosine kinase, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001376
Physical Location & Seq
Reverse (-)
6411200 .. 6414474
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164430.1

Sequence Viewer

Length: 2214 bp
ATGGCACAGAGACCAGAACAAGTAGAAGTCGAGGTCGACGGTACCGGAGAAAGAAGCGCGAGAAACACAGTTGGTGGGGAGTCTTTCCGGCGAAGAAGCAAAGATCAGGCTGCACCCAACTCGGGTGGACCTCAGGTTCTGAGGTGCAGCTCAGGCGCTTCGTTTTCTAGTAAAGGTTGGAAACCCCCAATGAGCAAGACCAGGTCGAGGCTTATTGACCCACTTGAGGAACGGTGTCCCAAATCCGATAGGTTGGCCCATTCCGGACGACTGGTCGGAAAAGATGAAGAGGACGATGGTTTTGACGTTGATGATATACCCGAAGAGTACAAGAGGATGAAGTTCAACACTTTGACGTTACTTCAATGGGTGAGTCTTGTTTGTGTTTTAGGTGCTTTGCTTTGTAATCTTTGGATTCCGATGATAAGGAGACTAAGGCCGTGGGATCTTCCGATTTGGAAATGGGAGTTGTTGGTTTTGGCATTGATTTGTGGGCGTTTGGTTTCGGGTTGGGGGATTCGGGTGGTTGTGTTCTTTGTGGAGAGAAATTTTCTGATGAGGAAAAGGGTGTTGTATTTTGTTTATGGGTTGCGAAAGGCTGTGCAGAATTGTCTCTGGTTGGGTTTGGTTTTGGTTGTGTGGCATTTGATTTTTGATAAAAAGGTTGAGAAGGAGACCAAGAGTAGGATCTTACCTTATGTGACTAGGATACTGGTGTGTTTTCTGGTTGCTACTTTGATTTGGCTTGTGAAAACATTACTTGTCAAGGTCCTTGCTTTGTCTTTCCATGTCAATGCCTTTTTCGAACGAATTCGGGAGGCTTTGTTCAATCAGTATGTGATTGAGACGCTTTCTGGTCCTCCTTTGTTCGAAAGGCAGCAGATACAAGAGGAAGAGGAAAAGGCTGCCGCAGAGATTAGGGAGTTTCAGAAGGCAGGGGCTCGTATGCCTCGCGAGCTCAGGGCAACTCTTCTTCCAAGGAATGGGCCGGTTGTGGGGAGTGGTGGACTGCAGAGTAGCCCCACAATAGGGAGGAGTCCTAGATTTTCTTCTACACCTACAACGCCTAAGTCTGTGAAGCAAGATGAGGAGATCCCAGTTAACCACTTGAATAAGCTAAATCAGAAGAATGTATCAGCTTGGAATATGAGGCGAATGATGAATATCATCCGACACGGCTCCTTAACCACTCTAGATGAACAGATACTGGATGTTGAGGACGACTCTTCTGTGGAGATCAAAACTGAATGCCAGGCAAAAGAGGCAGCGAGGAAGATATTCCTCAAAGTAGCCAAGCCAGGGGCCAAATGCATTTTCCTTGACGATCTGATGCTTTTTATGAATAAAGATGAAGCCTTGAAGACAATTGTTCTCTTTGGAGCAGCAAGTGAACATGATGGGATCAGCAAGTCTTCTCTTAAACATTGGATGGTGAGAGCATTTAGAGAGAGAAGAGCTCTATCATTGTCTCTCAATGATACGAAAACAGCAGTGGATGAACTCCACAATTTATTAAACATTCTTGTGGGTGTGATCATTGTGATAACCTGGCTGATTATACTTGGGATTCCAATCACTCATTTCCTTGTGTTCATAAGCTCACAGATTCTTTTGGTTGTATTTATCTTTGGGAACACATGCAAGACAGTTTTTGAAGCAATAATCTTTCTGTTTGTAATGCACCCATATGATGTGGGTGATCGTTGTGAGGTGGATGGAGTCCAGTTGGTAGTTGAGGAGATGAATCTATTAACAACAGTCTTTCTGAAGTTTGATAACCAGAAGGTTATATACCCCAACAGTGTTCTTGCTACCAAGCCAATTGCTAATTATTATCGTAGTCCTGACATGGGAGATGCTGTTGATTTCTGTGTTCATATATCAACTCCATTGGAGAAGTTGAACATTGTGAAAGAAAAAATCATAGGGTACATTGAAACCAGGACTGACCACTGGCACCCAGCACCAATGGTGGTCTTGAGGGATGTGGAGGAACTGAACAAACTGAAGATATCAGTGTGGCTTACACACAAGATGAACCACCAGGACATGGTGGAGAGATGGAGTCGGAGAGCCCTTTTGATTGAAGCCATGGTCAAAGTGTTCAAAGAGCTTGACATTCAGTATCGCTTGCTGCCTATCGATGTCAATGTCCGAAACATGCCAAGTTTAACCTCCAAGCCCCCTTCAAATTGGACTGCTTGTGCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MS_channel Pkinase_Tyr

Protein Analysis

737

Amino Acids

84.22

Weight (kDa)

9.06

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MS_channel_2nd PF00924 555 - 612 5.5e-11 Mechanosensitive ion channel, beta-domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 41
AccB1I GGYRCC 2 cut(s) 41, 1956
AccB7I CCANNNNNTGG 2 cut(s) 983, 2050
AccI GTMKAC 1 cut(s) 36
AccII CGCG 2 cut(s) 59, 954
AccIII TCCGGA 1 cut(s) 263
AciI CCGC 1 cut(s) 909
AclWI GGATC 4 cut(s) 453, 695, 1087, 1409
AcsI RAATTY 2 cut(s) 547, 810
AcuI CTGAAG 2 cut(s) 1787, 2027
AfaI GTAC 3 cut(s) 43, 329, 1931
AhdI GACNNNNNGTC 1 cut(s) 272
AjnI CCWGG 6 cut(s) 200, 1251, 1297, 1547, 1940, 2043
AjuI GAANNNNNNNTTGG 2 cut(s) 1298, 1330
AloI GAACNNNNNNTCC 4 cut(s) 120, 152, 809, 841
AluBI AGCT 7 cut(s) 150, 958, 1117, 1139, 1457, 1599, 2113
AluI AGCT 7 cut(s) 150, 958, 1117, 1139, 1457, 1599, 2113
Alw21I GWGCWC 3 cut(s) 960, 1459, 2209
Alw26I GTCTC 6 cut(s) 4, 424, 617, 668, 839, 1473
AlwI GGATC 4 cut(s) 453, 695, 1087, 1409
AlwNI CAGNNNCTG 2 cut(s) 139, 1207
Ama87I CYCGRG 1 cut(s) 121
Aor13HI TCCGGA 1 cut(s) 263
AoxI GGCC 4 cut(s) 255, 437, 986, 1302
ApeKI GCWGC 7 cut(s) 110, 147, 877, 905, 1265, 1382, 2134
ApoI RAATTY 2 cut(s) 547, 810
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp700I GAANNNNTTC 2 cut(s) 810, 1277
Asp718I GGTACC 1 cut(s) 41
AspLEI GCGC 2 cut(s) 59, 158
AspS9I GGNCC 6 cut(s) 128, 256, 769, 857, 986, 1302
AsuHPI GGTGA 3 cut(s) 382, 1444, 1709
AsuII TTCGAA 2 cut(s) 804, 870
AvaI CYCGRG 1 cut(s) 121
AvaII GGWCC 3 cut(s) 128, 769, 857
AxyI CCTNAGG 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 701, 734
BanI GGYRCC 2 cut(s) 41, 1956
BanII GRGCYC 4 cut(s) 943, 960, 1459, 2077
BarI GAAGNNNNNNTAC 2 cut(s) 1775, 1807
BbsI GAAGAC 2 cut(s) 1367, 1404
Bbv12I GWGCWC 3 cut(s) 960, 1459, 2209
BbvI GCAGC 7 cut(s) 97, 159, 889, 892, 1277, 1394, 2121
BccI CCATC 5 cut(s) 290, 1391, 1423, 1709, 2055
BceAI ACGGC 2 cut(s) 424, 1192
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
BciVI GTATCC 1 cut(s) 702
BclI TGATCA 1 cut(s) 1533
BcoDI GTCTC 6 cut(s) 4, 424, 617, 668, 839, 1473
BfaI CTAG 4 cut(s) 168, 705, 1041, 1193
BfmI CTRYAG 1 cut(s) 1010
BfoI RGCGCY 1 cut(s) 159
BfuI GTATCC 1 cut(s) 702
BisI GCNGC 8 cut(s) 111, 148, 878, 906, 909, 1266, 1383, 2135
BlsI GCNGC 8 cut(s) 112, 149, 879, 907, 910, 1267, 1384, 2136
Bme1390I CCNGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
Bme18I GGWCC 3 cut(s) 128, 769, 857
BmeRI GACNNNNNGTC 1 cut(s) 272
BmeT110I CYCGRG 1 cut(s) 121
BmgT120I GGNCC 6 cut(s) 128, 256, 769, 857, 986, 1302
BmiI GGNNCC 4 cut(s) 43, 1180, 1303, 1958
BmrFI CCNGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
BmrI ACTGGG 1 cut(s) 1091
BmsI GCATC 2 cut(s) 1320, 1846
BmuI ACTGGG 1 cut(s) 1091
BpiI GAAGAC 2 cut(s) 1367, 1404
BplI GAGNNNNNCTC 4 cut(s) 1208, 1240, 1441, 1473
Bpu10I CCTNAGC 2 cut(s) 151, 959
Bpu14I TTCGAA 2 cut(s) 804, 870
BpuEI CTTGAG 2 cut(s) 245, 1999
Bsa29I ATCGAT 1 cut(s) 2142
BsaBI GATNNNNATC 2 cut(s) 1163, 1571
BsaI GGTCTC 2 cut(s) 4, 668
BsaJI CCNNGG 4 cut(s) 440, 977, 1298, 2091
BsaWI WCCGGW 2 cut(s) 44, 263
BsaXI ACNNNNNCTCC 4 cut(s) 809, 839, 1845, 1875
Bse118I RCCGGY 1 cut(s) 988
Bse1I ACTGG 6 cut(s) 276, 717, 1097, 1212, 1723, 1958
Bse21I CCTNAGG 1 cut(s) 132
Bse8I GATNNNNATC 2 cut(s) 1163, 1571
BseAI TCCGGA 1 cut(s) 263
BseBI CCWGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
BseCI ATCGAT 1 cut(s) 2142
BseDI CCNNGG 4 cut(s) 440, 977, 1298, 2091
BseGI GGATG 7 cut(s) 342, 1167, 1216, 1434, 1501, 1720, 1990
BseJI GATNNNNATC 2 cut(s) 1163, 1571
BseMII CTCAG 4 cut(s) 131, 146, 165, 973
BseNI ACTGG 6 cut(s) 276, 717, 1097, 1212, 1723, 1958
BseRI GAGGAG 3 cut(s) 1048, 1103, 1751
BseXI GCAGC 7 cut(s) 97, 159, 889, 892, 1277, 1394, 2121
BseYI CCCAGC 1 cut(s) 1960
BsgI GTGCAG 3 cut(s) 96, 166, 623
Bsh1236I CGCG 2 cut(s) 59, 954
BshFI GGCC 4 cut(s) 257, 439, 988, 1304
BshNI GGYRCC 2 cut(s) 41, 1956
BshVI ATCGAT 1 cut(s) 2142
BsiHKAI GWGCWC 3 cut(s) 960, 1459, 2209
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 4 cut(s) 45, 88, 264, 989
BslFI GGGAC 1 cut(s) 222
BsmAI GTCTC 6 cut(s) 4, 424, 617, 668, 839, 1473
BsmBI CGTCTC 1 cut(s) 839
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 1253
BsnI GGCC 4 cut(s) 257, 439, 988, 1304
Bso31I GGTCTC 2 cut(s) 4, 668
BsoBI CYCGRG 1 cut(s) 121
Bsp119I TTCGAA 2 cut(s) 804, 870
Bsp1286I GDGCHC 5 cut(s) 943, 960, 1459, 2077, 2209
Bsp13I TCCGGA 1 cut(s) 263
Bsp143I GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
Bsp19I CCATGG 1 cut(s) 2091
Bsp68I TCGCGA 1 cut(s) 954
BspACI CCGC 1 cut(s) 909
BspANI GGCC 4 cut(s) 257, 439, 988, 1304
BspCNI CTCAG 4 cut(s) 132, 145, 164, 972
BspDI ATCGAT 1 cut(s) 2142
BspEI TCCGGA 1 cut(s) 263
BspFNI CGCG 2 cut(s) 59, 954
BspLI GGNNCC 4 cut(s) 43, 1180, 1303, 1958
BspMAI CTGCAG 1 cut(s) 1014
BspPI GGATC 4 cut(s) 453, 695, 1087, 1409
BspQI GCTCTTC 1 cut(s) 1447
BspT104I TTCGAA 2 cut(s) 804, 870
BspT107I GGYRCC 2 cut(s) 41, 1956
BspTNI GGTCTC 2 cut(s) 4, 668
BsrFI RCCGGY 1 cut(s) 988
BsrI ACTGG 6 cut(s) 276, 717, 1097, 1212, 1723, 1958
BssAI RCCGGY 1 cut(s) 988
BssECI CCNNGG 4 cut(s) 440, 977, 1298, 2091
BssMI GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
BssT1I CCWWGG 2 cut(s) 977, 2091
Bst2UI CCWGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
Bst4CI ACNGT 6 cut(s) 41, 70, 234, 1648, 1759, 1802
Bst6I CTCTTC 6 cut(s) 282, 318, 888, 975, 1231, 1447
BstBI TTCGAA 2 cut(s) 804, 870
BstC8I GCNNGC 2 cut(s) 956, 2132
BstDEI CTNAG 6 cut(s) 132, 140, 151, 434, 959, 1068
BstDSI CCRYGG 2 cut(s) 440, 2091
BstF5I GGATG 7 cut(s) 342, 1167, 1216, 1434, 1501, 1720, 1990
BstFNI CGCG 2 cut(s) 59, 954
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 2 cut(s) 59, 158
BstKTI GATC 9 cut(s) 106, 448, 690, 1095, 1239, 1327, 1404, 1536, 1702
BstMAI GTCTC 6 cut(s) 4, 424, 617, 668, 839, 1473
BstMBI GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
BstMWI GCNNNNNNNGC 3 cut(s) 153, 955, 1262
BstNI CCWGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
BstNSI RCATGY 2 cut(s) 1641, 2164
BstSCI CCNGG 6 cut(s) 200, 1251, 1297, 1547, 1940, 2043
BstSFI CTRYAG 1 cut(s) 1010
BstUI CGCG 2 cut(s) 59, 954
BstV1I GCAGC 7 cut(s) 97, 159, 889, 892, 1277, 1394, 2121
BstV2I GAAGAC 2 cut(s) 1367, 1404
BstX2I RGATCY 3 cut(s) 445, 687, 1092
BstYI RGATCY 3 cut(s) 445, 687, 1092
Bsu15I ATCGAT 1 cut(s) 2142
Bsu36I CCTNAGG 1 cut(s) 132
BsuI GTATCC 1 cut(s) 702
BsuRI GGCC 4 cut(s) 257, 439, 988, 1304
BsuTUI ATCGAT 1 cut(s) 2142
BtgI CCRYGG 2 cut(s) 440, 2091
BtsCI GGATG 7 cut(s) 342, 1167, 1216, 1434, 1501, 1720, 1990
BtsI GCAGTG 1 cut(s) 1497
BtsIMutI CAGTG 4 cut(s) 1497, 1807, 1951, 2022
BtuMI TCGCGA 1 cut(s) 954
Cac8I GCNNGC 2 cut(s) 956, 2132
CaiI CAGNNNCTG 2 cut(s) 139, 1207
CfoI GCGC 2 cut(s) 59, 158
Cfr10I RCCGGY 1 cut(s) 988
Cfr13I GGNCC 6 cut(s) 128, 256, 769, 857, 986, 1302
ClaI ATCGAT 1 cut(s) 2142
CseI GACGC 1 cut(s) 856
CsiI ACCWGGT 1 cut(s) 200
Csp6I GTAC 3 cut(s) 42, 328, 1930
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 7 cut(s) 788, 1394, 1638, 1849, 2050, 2092, 2161
CviQI GTAC 3 cut(s) 42, 328, 1930
DdeI CTNAG 6 cut(s) 132, 140, 151, 434, 959, 1068
DpnI GATC 9 cut(s) 105, 447, 689, 1094, 1238, 1326, 1403, 1535, 1701
DpnII GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
DriI GACNNNNNGTC 1 cut(s) 272
Eam1104I CTCTTC 6 cut(s) 282, 318, 888, 975, 1231, 1447
Eam1105I GACNNNNNGTC 1 cut(s) 272
EarI CTCTTC 6 cut(s) 282, 318, 888, 975, 1231, 1447
Ecl136II GAGCTC 2 cut(s) 958, 1457
Eco130I CCWWGG 2 cut(s) 977, 2091
Eco24I GRGCYC 4 cut(s) 943, 960, 1459, 2077
Eco31I GGTCTC 2 cut(s) 4, 668
Eco32I GATATC 1 cut(s) 2013
Eco47I GGWCC 3 cut(s) 128, 769, 857
Eco53kI GAGCTC 2 cut(s) 958, 1457
Eco57I CTGAAG 2 cut(s) 1787, 2027
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 121
EcoICRI GAGCTC 2 cut(s) 958, 1457
EcoO109I RGGNCCY 1 cut(s) 769
EcoRI GAATTC 1 cut(s) 810
EcoRII CCWGG 6 cut(s) 200, 1251, 1297, 1547, 1940, 2043
EcoRV GATATC 1 cut(s) 2013
EcoT14I CCWWGG 2 cut(s) 977, 2091
EcoT22I ATGCAT 1 cut(s) 1313
EcoT38I GRGCYC 4 cut(s) 943, 960, 1459, 2077
ErhI CCWWGG 2 cut(s) 977, 2091
Esp3I CGTCTC 1 cut(s) 839
FaeI CATG 7 cut(s) 791, 1397, 1641, 1852, 2053, 2095, 2164
FaqI GGGAC 1 cut(s) 222
FatI CATG 7 cut(s) 787, 1393, 1637, 1848, 2049, 2091, 2160
FauNDI CATATG 1 cut(s) 1687
FbaI TGATCA 1 cut(s) 1533
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 8 cut(s) 111, 148, 878, 906, 909, 1266, 1383, 2135
FokI GGATG 7 cut(s) 349, 1154, 1223, 1441, 1508, 1727, 1997
FriOI GRGCYC 4 cut(s) 943, 960, 1459, 2077
Fsp4HI GCNGC 8 cut(s) 111, 148, 878, 906, 909, 1266, 1383, 2135
FspBI CTAG 4 cut(s) 168, 705, 1041, 1193
GlaI GCGC 2 cut(s) 58, 157
GluI GCNGC 8 cut(s) 111, 148, 878, 906, 909, 1266, 1383, 2135
GsaI CCCAGC 1 cut(s) 1964
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 4 cut(s) 257, 439, 988, 1304
HapII CCGG 4 cut(s) 45, 88, 264, 989
HgaI GACGC 1 cut(s) 856
HhaI GCGC 2 cut(s) 59, 158
Hin1II CATG 7 cut(s) 791, 1397, 1641, 1852, 2053, 2095, 2164
Hin6I GCGC 2 cut(s) 57, 156
HinP1I GCGC 2 cut(s) 57, 156
HincII GTYRAC 2 cut(s) 37, 1102
HindII GTYRAC 2 cut(s) 37, 1102
HpaI GTTAAC 1 cut(s) 1102
HpaII CCGG 4 cut(s) 45, 88, 264, 989
HphI GGTGA 3 cut(s) 382, 1444, 1709
Hpy166II GTNNAC 5 cut(s) 37, 128, 1007, 1102, 1391
Hpy188III TCNNGA 6 cut(s) 264, 815, 953, 1193, 1844, 1978
Hpy8I GTNNAC 5 cut(s) 37, 128, 1007, 1102, 1391
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 664, 925, 1777, 2196
HpyCH4III ACNGT 6 cut(s) 41, 70, 234, 1648, 1759, 1802
HpyCH4IV ACGT 2 cut(s) 306, 356
HpyCH4V TGCA 7 cut(s) 113, 147, 604, 1012, 1311, 1641, 1681
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 955, 1262
HpyF3I CTNAG 6 cut(s) 132, 140, 151, 434, 959, 1068
HpySE526I ACGT 2 cut(s) 306, 356
Hsp92II CATG 7 cut(s) 791, 1397, 1641, 1852, 2053, 2095, 2164
HspAI GCGC 2 cut(s) 57, 156
Kpn2I TCCGGA 1 cut(s) 263
KpnI GGTACC 1 cut(s) 45
Ksp22I TGATCA 1 cut(s) 1533
KspAI GTTAAC 1 cut(s) 1102
Kzo9I GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
LguI GCTCTTC 1 cut(s) 1447
LmnI GCTCC 3 cut(s) 1184, 1379, 2212
Lsp1109I GCAGC 7 cut(s) 97, 159, 889, 892, 1277, 1394, 2121
LweI GCATC 2 cut(s) 1320, 1846
MabI ACCWGGT 1 cut(s) 200
MaeI CTAG 4 cut(s) 168, 705, 1041, 1193
MaeII ACGT 2 cut(s) 306, 356
MaeIII GTNAC 2 cut(s) 357, 700
MalI GATC 9 cut(s) 105, 447, 689, 1094, 1238, 1326, 1403, 1535, 1701
MboI GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
MfeI CAATTG 2 cut(s) 1365, 1821
MflI RGATCY 3 cut(s) 445, 687, 1092
MhlI GDGCHC 5 cut(s) 943, 960, 1459, 2077, 2209
MluCI AATT 8 cut(s) 547, 607, 810, 1365, 1507, 1821, 1828, 2191
MlyI GAGTC 6 cut(s) 89, 382, 1045, 1217, 1728, 2074
MmeI TCCRAC 4 cut(s) 158, 256, 1195, 2048
Mph1103I ATGCAT 1 cut(s) 1313
MroI TCCGGA 1 cut(s) 263
MroXI GAANNNNTTC 2 cut(s) 810, 1277
MseI TTAA 6 cut(s) 1101, 1184, 1419, 1514, 1751, 2171
MslI CAYNNNNRTG 3 cut(s) 792, 1523, 1686
MspI CCGG 4 cut(s) 45, 88, 264, 989
MspR9I CCNGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
MunI CAATTG 2 cut(s) 1365, 1821
Mva1269I GAATGC 1 cut(s) 1253
MvaI CCWGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
MvnI CGCG 2 cut(s) 59, 954
MwoI GCNNNNNNNGC 3 cut(s) 153, 955, 1262
NcoI CCATGG 1 cut(s) 2091
NdeI CATATG 1 cut(s) 1687
NdeII GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
NlaIII CATG 7 cut(s) 791, 1397, 1641, 1852, 2053, 2095, 2164
NlaIV GGNNCC 4 cut(s) 43, 1180, 1303, 1958
NmuCI GTSAC 1 cut(s) 700
NruI TCGCGA 1 cut(s) 954
NsiI ATGCAT 1 cut(s) 1313
NspI RCATGY 2 cut(s) 1641, 2164
NspV TTCGAA 2 cut(s) 804, 870
PciSI GCTCTTC 1 cut(s) 1447
PctI GAATGC 1 cut(s) 1253
PdmI GAANNNNTTC 2 cut(s) 810, 1277
PfeI GAWTC 5 cut(s) 415, 517, 1567, 1606, 1744
PflFI GACNNNGTC 1 cut(s) 202
PflMI CCANNNNNTGG 2 cut(s) 983, 2050
PkrI GCNGC 8 cut(s) 112, 149, 879, 907, 910, 1267, 1384, 2136
PleI GAGTC 6 cut(s) 88, 381, 1044, 1217, 1727, 2073
PpsI GAGTC 6 cut(s) 88, 381, 1044, 1217, 1727, 2073
PpuMI RGGWCCY 1 cut(s) 769
Psp124BI GAGCTC 2 cut(s) 960, 1459
Psp5II RGGWCCY 1 cut(s) 769
Psp6I CCWGG 6 cut(s) 200, 1251, 1297, 1547, 1940, 2043
PspFI CCCAGC 1 cut(s) 1960
PspGI CCWGG 6 cut(s) 200, 1251, 1297, 1547, 1940, 2043
PspN4I GGNNCC 4 cut(s) 43, 1180, 1303, 1958
PspPI GGNCC 6 cut(s) 128, 256, 769, 857, 986, 1302
PspPPI RGGWCCY 1 cut(s) 769
PstI CTGCAG 1 cut(s) 1014
PstNI CAGNNNCTG 2 cut(s) 139, 1207
PsuI RGATCY 3 cut(s) 445, 687, 1092
PsyI GACNNNGTC 1 cut(s) 202
RruI TCGCGA 1 cut(s) 954
RsaI GTAC 3 cut(s) 43, 329, 1931
RsaNI GTAC 3 cut(s) 42, 328, 1930
RseI CAYNNNNRTG 3 cut(s) 792, 1523, 1686
SacI GAGCTC 2 cut(s) 960, 1459
SalI GTCGAC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 1447
SaqAI TTAA 6 cut(s) 1101, 1184, 1419, 1514, 1751, 2171
SatI GCNGC 8 cut(s) 111, 148, 878, 906, 909, 1266, 1383, 2135
Sau3AI GATC 9 cut(s) 103, 445, 687, 1092, 1236, 1324, 1401, 1533, 1699
Sau96I GGNCC 6 cut(s) 128, 256, 769, 857, 986, 1302
SchI GAGTC 6 cut(s) 89, 382, 1045, 1217, 1728, 2074
ScrFI CCNGG 6 cut(s) 202, 1253, 1299, 1549, 1942, 2045
SduI GDGCHC 5 cut(s) 943, 960, 1459, 2077, 2209
SexAI ACCWGGT 1 cut(s) 200
SfaNI GCATC 2 cut(s) 1320, 1846
SfcI CTRYAG 1 cut(s) 1010
SfuI TTCGAA 2 cut(s) 804, 870
SinI GGWCC 3 cut(s) 128, 769, 857
SmiMI CAYNNNNRTG 3 cut(s) 792, 1523, 1686
SmlI CTYRAG 2 cut(s) 224, 1978
SmoI CTYRAG 2 cut(s) 224, 1978
Sse9I AATT 8 cut(s) 547, 607, 810, 1365, 1507, 1821, 1828, 2191
SsiI CCGC 1 cut(s) 909
SspMI CTAG 4 cut(s) 168, 705, 1041, 1193
SstI GAGCTC 2 cut(s) 960, 1459
StyD4I CCNGG 6 cut(s) 200, 1251, 1297, 1547, 1940, 2043
StyI CCWWGG 2 cut(s) 977, 2091
TaaI ACNGT 6 cut(s) 41, 70, 234, 1648, 1759, 1802
TaiI ACGT 2 cut(s) 309, 359
TaqI TCGA 6 cut(s) 30, 36, 206, 804, 870, 2142
TasI AATT 8 cut(s) 547, 607, 810, 1365, 1507, 1821, 1828, 2191
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 911
TfiI GAWTC 5 cut(s) 415, 517, 1567, 1606, 1744
Tru1I TTAA 6 cut(s) 1101, 1184, 1419, 1514, 1751, 2171
Tru9I TTAA 6 cut(s) 1101, 1184, 1419, 1514, 1751, 2171
TscAI CASTG 4 cut(s) 1497, 1807, 1958, 2022
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 7 cut(s) 110, 147, 877, 905, 1265, 1382, 2134
Tsp45I GTSAC 1 cut(s) 700
TspRI CASTG 4 cut(s) 1497, 1807, 1958, 2022
Tth111I GACNNNGTC 1 cut(s) 202
Van91I CCANNNNNTGG 2 cut(s) 983, 2050
VpaK11BI GGWCC 3 cut(s) 128, 769, 857
XapI RAATTY 2 cut(s) 547, 810
XbaI TCTAGA 1 cut(s) 1192
XceI RCATGY 2 cut(s) 1641, 2164
XmiI GTMKAC 1 cut(s) 36
XmnI GAANNNNTTC 2 cut(s) 810, 1277
XspI CTAG 4 cut(s) 168, 705, 1041, 1193
Zsp2I ATGCAT 1 cut(s) 1313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.