Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Reverse (-)

12530007 .. 12534290

Gene Structure 4284 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00010020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGATTTTTCACTGAAGAAGTCATTCAAATCCCATGGGTCGAGCAAACACATGAGAAAGATCTCTGCCGGAGCAGTAGAAGATAACAGCCACGAGGAGCTCCCGATTCTAATTGATCACCAGAACCAGGCAAGCATCGCCTCAGATTCGCCGGGCCACCACGGCCGAGAGGTCATCGTCAAGATCGACGAGGGCGATTCCTCCGCAACAACCATCAGAGAGAACGTCAGGGAAGTGGAGCATGGCGGGACGATTTGGCGGGAATCGAGTTTAGATTTTTGGACGGAGGACGACGGGAGCACTGTCCCCGCCGATGGAAGCGCCGGCAGCGGGGGCTTTCATTTCGTGCAGAGGGGGAAGCAGGCTGCGCCGGCGGCAGAGGAGGATCCGCCATCGAAGCTGATTGGGCAGTACCTTAAGAAGCAGAGAGCCTCGGGCGGCGACGTGTCGTTGGACATGGATTTGGAGATGGACGAGCTACAAAAACCGCCGCTGGCGGAGCCTCCGAGGATCAGCCAGGCGTCGCGGGAGCTCAAGGTGTCGTTCCAGTCGCCGGCACGGTCGGAGCTCCCCGAGGCGGCGAACGATTCTGTTCGGAGGCGAAACAAGGACTCGGTGGTTGACGACGAACGACGTCGTAGCAGTCGGTTGAGTAACGGCGGAGCCGATGTGATTAGGTGCTCGTCAAACAAATCGTTTCGGCGAGAGGCTTCGTTTCAGAACAACAATTTGTTGAGGCTAAAGACCAAGTCTAGATTGATAGACCCGCCTGAAAGCTGGGACGAGGTTCGGGTCCCGAAATCGGGTCCGATGAAGTCAGGGATGTTGGGGAAAGGCGGCGACGATGACGACGAGGACCCGTTTTTGGAAGAGGATCTGCCTGATGAGTACAAGAGGGTAAAATTAGATGCATTGACTCTGCTCCAATGGCTTAGTTTGGTTTTGATCATTGGCGCCTTGGTTTGCACGCTTTCTCTAAGAGTTTTGAGGCAGAAGCATCTGTGGAAATTGAAGCTGTGGAAATGGGAGGTGCTGATTTTGGTTTCGATTTGCGGGAGGCTGGTTTCGGGTTGGGTGATAAAGATCATGGTGTTCTTCGTGGAAAGGAATTTCCTTCTGCGCAAGAGGGTGTTGTATTTTGTTTATGGGATCAGAAGGGCTGTGCAGAACTGTATTTGGCTTGGCTTTGTTTTGACGGCTTGGCATTTCATGCTAATCAGGAAGGTTGAGCAGGAAACCAATAGCAAAATACTAGAGTATGTGACGAAAGGTTTGCTTTGTCTCTTGATAGGGGTTTTGCTATGGTTGTTAAAGACCCTGGTTGTGAAGATCAAATGCATTCCCTCTGCGAGAATCGGGAGGGCGGTTGGGAGTGGCTCTGGGAGAAGCGGGAGGTTCATTGCTGCAAGTGGAGGGCTTGTGGGGAAGAGTGGCAGGTTTTCAAGGCCAACAATGTCCAAGAAGTCAGAGGAGGCTGCAATCACCATTGATCACTTGCATAGGCTCAATCCAAAGAATGTATCTGCTTGGAATATGAAGAGGCTAATGAACATTGTTAGAAAAGGGCATCTTACGACACTCGATGAGCAAATTCTGGATTCCAGCTATCCGGATGAAAGCGATACTAAGATCAAAAGTGAAGTTGAGGCAAAGGCTGCAGCTAAAAAGATATTTCGCAATGTGGCTCGGCCTGGCTCAAAGCACATCTACCTAGAGGATTTGTTGCGTTTCATGGGAGAAGAGGAGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCCGAGACTCAGAGAATCCGCAAAAATTCCTTGAAAAATTGGGTGGTCAACTGCTTTAGAGAACGGAGAGCACTTTCTTTGACACTGAATGACACCAAAACAGCGGTGAACAGACTTCGACACGTGGTGGACGTAATAGTTGGCATTGTTATTATAGTTATATGGCTTCTTGTGCTGGATATTGTCACCAGCGCCAATCTTGTTTATGCAACTTCTCAGCTTGTGGTCGTGGCATTTGTTTTTGGTAACACTTGCAGGACGGTATTTGAAGCAGTCATATTTTTATTCGTGATGCACCCCTTTGACGTGGGAGATCGATGTGAAATTGAAGGAGTGCAGATGGTTGTAGAAGAAATGAATATCTTGACTACAGTTTTTCTAAGGTATGACAACACGAAAATCGTGTACCCAAACAGTACCCTTTCAACTAAGCCCATCAATAACTTCTATCGTAGTCCGGACACAGGAGATGCTGTTGAGTTCTGTGTCCATATATCTACTCCGGCAGATAAGATTGCTGTTATGAAACAGAGAATGATAAGTTACATTGAAAATAAAAAGGAGCACTGGTATCCTCAAGTACAGATCGTATTGAAGGATGTAGAAGAATTGAATAAGATGAGGTATGCATTGTGGCTGACACATAAGATGAACTTCCAAGATGTGGGAGAAAAGTGGGAAAGAAGAGCCCTTTTACTTGAAGAGATGGTAAGGATCTTCCAGGAGCTGGACATTCAGTATCGTTTGCTGCCTATTGACATAAATGTCCGTGCCATGCCTCCCACAACCACCAATGGTCTGCCTCCCTCAAATTTTTCAGCAACCACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

870

Amino Acids

98.52

Weight (kDa)

9.06

Isoelectric Point (pI)

43.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	686 - 742	3.7e-11	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2543
AatII	GACGTC	1 cut(s)	637
Acc16I	TGCGCA	1 cut(s)	1121
Acc36I	ACCTGC	1 cut(s)	1425
AccB1I	GGYRCC	1 cut(s)	953
AccB7I	CCANNNNNTGG	2 cut(s)	2443, 2506
AccII	CGCG	1 cut(s)	526
AccIII	TCCGGA	2 cut(s)	1609, 2236
AclWI	GGATC	6 cut(s)	380, 393, 518, 882, 1157, 2501
AcoI	YGGCCR	1 cut(s)	163
AcsI	RAATTY	4 cut(s)	1108, 1590, 1804, 2590
AcuI	CTGAAG	1 cut(s)	35
AcvI	CACGTG	1 cut(s)	1903
AcyI	GRCGYC	3 cut(s)	521, 634, 954
AdeI	CACNNNGTG	1 cut(s)	1906
AfaI	GTAC	5 cut(s)	413, 890, 2186, 2197, 2361
AflII	CTTAAG	1 cut(s)	416
AflIII	ACRYGT	2 cut(s)	444, 1900
AhdI	GACNNNNNGTC	1 cut(s)	1761
AjiI	CACGTC	2 cut(s)	445, 2086
AjnI	CCWGG	5 cut(s)	126, 516, 1317, 1690, 2499
Alw21I	GWGCWC	7 cut(s)	102, 302, 534, 570, 683, 1852, 2346
Alw26I	GTCTC	3 cut(s)	1286, 1769, 1778
AlwI	GGATC	6 cut(s)	380, 393, 518, 882, 1157, 2501
AlwNI	CAGNNNCTG	1 cut(s)	2506
Ama87I	CYCGRG	2 cut(s)	433, 572
Aor13HI	TCCGGA	2 cut(s)	1609, 2236
AoxI	GGCC	5 cut(s)	154, 163, 1445, 1688, 1776
ApeKI	GCWGC	7 cut(s)	327, 365, 1403, 1475, 1655, 1658, 2527
ApoI	RAATTY	4 cut(s)	1108, 1590, 1804, 2590
ArsI	GACNNNNNNTTYG	2 cut(s)	1255, 1287
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	5 cut(s)	323, 370, 956, 1122, 1973
AspS9I	GGNCC	5 cut(s)	154, 793, 806, 856, 1776
AsuC2I	CCSGG	1 cut(s)	153
AsuHPI	GGTGA	5 cut(s)	110, 1087, 1474, 1897, 1957
AvaI	CYCGRG	2 cut(s)	433, 572
AvaII	GGWCC	3 cut(s)	793, 806, 856
BamHI	GGATCC	1 cut(s)	385
BanI	GGYRCC	1 cut(s)	953
BanII	GRGCYC	4 cut(s)	102, 534, 570, 2470
BauI	CACGAG	1 cut(s)	92
BbrPI	CACGTG	1 cut(s)	1903
Bbv12I	GWGCWC	7 cut(s)	102, 302, 534, 570, 683, 1852, 2346
BbvI	GCAGC	7 cut(s)	339, 352, 1390, 1462, 1642, 1670, 2514
BccI	CCATC	7 cut(s)	221, 308, 400, 463, 2113, 2222, 2479
BceAI	ACGGC	3 cut(s)	178, 673, 1212
BcgI	CGANNNNNNTGC	2 cut(s)	1255, 1289
BciT130I	CCWGG	5 cut(s)	128, 518, 1319, 1692, 2501
BciVI	GTATCC	1 cut(s)	2361
BclI	TGATCA	3 cut(s)	115, 945, 1489
BcnI	CCSGG	1 cut(s)	153
BcoDI	GTCTC	3 cut(s)	1286, 1769, 1778
BfaI	CTAG	3 cut(s)	753, 1253, 1712
BfmI	CTRYAG	2 cut(s)	1656, 2148
BfoI	RGCGCY	3 cut(s)	324, 957, 1974
BfrI	CTTAAG	1 cut(s)	416
BfuAI	ACCTGC	1 cut(s)	1425
BfuI	GTATCC	1 cut(s)	2361
BglI	GCCNNNNNGGC	1 cut(s)	162
BglII	AGATCT	1 cut(s)	60
Bme1390I	CCNGG	6 cut(s)	128, 153, 518, 1319, 1692, 2501
Bme18I	GGWCC	3 cut(s)	793, 806, 856
BmeRI	GACNNNNNGTC	1 cut(s)	1761
BmeT110I	CYCGRG	2 cut(s)	433, 572
BmgBI	CACGTC	2 cut(s)	445, 2086
BmgT120I	GGNCC	5 cut(s)	154, 793, 806, 856, 1776
BmiI	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 858, 955, 1777
BmrFI	CCNGG	6 cut(s)	128, 153, 518, 1319, 1692, 2501
BmsI	GCATC	6 cut(s)	144, 898, 1006, 1576, 2061, 2239
BpuEI	CTTGAG	2 cut(s)	518, 2340
BpuMI	CCSGG	1 cut(s)	153
Bsa29I	ATCGAT	1 cut(s)	2095
BsaAI	YACGTR	1 cut(s)	1903
BsaBI	GATNNNNATC	1 cut(s)	1082
BsaHI	GRCGYC	3 cut(s)	521, 634, 954
BsaJI	CCNNGG	7 cut(s)	34, 160, 432, 506, 573, 957, 1317
BsaWI	WCCGGW	2 cut(s)	1609, 2236
BsaXI	ACNNNNNCTCC	8 cut(s)	654, 684, 1404, 1434, 2238, 2268, 2333, 2363
Bse118I	RCCGGY	3 cut(s)	323, 370, 553
Bse1I	ACTGG	2 cut(s)	547, 2351
Bse3DI	GCAATG	2 cut(s)	1398, 1684
Bse8I	GATNNNNATC	1 cut(s)	1082
BseAI	TCCGGA	2 cut(s)	1609, 2236
BseBI	CCWGG	5 cut(s)	128, 518, 1319, 1692, 2501
BseCI	ATCGAT	1 cut(s)	2095
BseDI	CCNNGG	7 cut(s)	34, 160, 432, 506, 573, 957, 1317
BseGI	GGATG	3 cut(s)	828, 1618, 2383
BseJI	GATNNNNATC	1 cut(s)	1082
BseMI	GCAATG	2 cut(s)	1398, 1684
BseMII	CTCAG	3 cut(s)	156, 1802, 2009
BseNI	ACTGG	2 cut(s)	547, 2351
BseRI	GAGGAG	4 cut(s)	110, 395, 1484, 1757
BseX3I	CGGCCG	1 cut(s)	163
BseXI	GCAGC	7 cut(s)	339, 352, 1390, 1462, 1642, 1670, 2514
BseYI	CCCAGC	1 cut(s)	777
BsgI	GTGCAG	3 cut(s)	368, 1184, 2135
Bsh1236I	CGCG	1 cut(s)	526
Bsh1285I	CGRYCG	2 cut(s)	166, 563
BshFI	GGCC	5 cut(s)	156, 165, 1447, 1690, 1778
BshNI	GGYRCC	1 cut(s)	953
BshVI	ATCGAT	1 cut(s)	2095
BsiEI	CGRYCG	2 cut(s)	166, 563
BsiHKAI	GWGCWC	7 cut(s)	102, 302, 534, 570, 683, 1852, 2346
BsiHKCI	CYCGRG	2 cut(s)	433, 572
BsiSI	CCGG	8 cut(s)	69, 152, 324, 371, 554, 1610, 2237, 2282
BslFI	GGGAC	4 cut(s)	262, 290, 779, 794
BsmAI	GTCTC	3 cut(s)	1286, 1769, 1778
BsmFI	GGGAC	4 cut(s)	262, 290, 779, 794
BsmI	GAATGC	1 cut(s)	1338
BsnI	GGCC	5 cut(s)	156, 165, 1447, 1690, 1778
BsoBI	CYCGRG	2 cut(s)	433, 572
Bsp1286I	GDGCHC	8 cut(s)	102, 302, 534, 570, 683, 1852, 2346, 2470
Bsp13I	TCCGGA	2 cut(s)	1609, 2236
Bsp19I	CCATGG	1 cut(s)	34
BspANI	GGCC	5 cut(s)	156, 165, 1447, 1690, 1778
BspCNI	CTCAG	3 cut(s)	155, 1801, 2008
BspDI	ATCGAT	1 cut(s)	2095
BspEI	TCCGGA	2 cut(s)	1609, 2236
BspFNI	CGCG	1 cut(s)	526
BspLI	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 858, 955, 1777
BspMAI	CTGCAG	1 cut(s)	1660
BspMI	ACCTGC	1 cut(s)	1425
BspPI	GGATC	6 cut(s)	380, 393, 518, 882, 1157, 2501
BspQI	GCTCTTC	1 cut(s)	2458
BspT107I	GGYRCC	1 cut(s)	953
BspTI	CTTAAG	1 cut(s)	416
BsrDI	GCAATG	2 cut(s)	1398, 1684
BsrFI	RCCGGY	3 cut(s)	323, 370, 553
BsrI	ACTGG	2 cut(s)	547, 2351
BssAI	RCCGGY	3 cut(s)	323, 370, 553
BssECI	CCNNGG	7 cut(s)	34, 160, 432, 506, 573, 957, 1317
BssNI	GRCGYC	3 cut(s)	521, 634, 954
BssSI	CACGAG	1 cut(s)	92
BssT1I	CCWWGG	2 cut(s)	34, 957
Bst2BI	CACGAG	1 cut(s)	92
Bst2UI	CCWGG	5 cut(s)	128, 518, 1319, 1692, 2501
Bst4CI	ACNGT	6 cut(s)	304, 561, 1172, 2041, 2152, 2195
Bst6I	CTCTTC	6 cut(s)	864, 1421, 1532, 1734, 2458, 2475
BstACI	GRCGYC	3 cut(s)	521, 634, 954
BstAFI	CTTAAG	1 cut(s)	416
BstAPI	GCANNNNNTGC	2 cut(s)	1210, 1655
BstBAI	YACGTR	1 cut(s)	1903
BstC8I	GCNNGC	7 cut(s)	133, 325, 363, 372, 495, 555, 968
BstDEI	CTNAG	8 cut(s)	142, 932, 977, 1626, 1788, 1995, 2159, 2208
BstDSI	CCRYGG	2 cut(s)	34, 160
BstF5I	GGATG	3 cut(s)	828, 1618, 2383
BstFNI	CGCG	1 cut(s)	526
BstH2I	RGCGCY	3 cut(s)	324, 957, 1974
BstHHI	GCGC	5 cut(s)	323, 370, 956, 1122, 1973
BstMAI	GTCTC	3 cut(s)	1286, 1769, 1778
BstMCI	CGRYCG	2 cut(s)	166, 563
BstNI	CCWGG	5 cut(s)	128, 518, 1319, 1692, 2501
BstSCI	CCNGG	6 cut(s)	126, 151, 516, 1317, 1690, 2499
BstSFI	CTRYAG	2 cut(s)	1656, 2148
BstUI	CGCG	1 cut(s)	526
BstV1I	GCAGC	7 cut(s)	339, 352, 1390, 1462, 1642, 1670, 2514
BstX2I	RGATCY	4 cut(s)	60, 385, 874, 2493
BstYI	RGATCY	4 cut(s)	60, 385, 874, 2493
BstZI	CGGCCG	1 cut(s)	163
Bsu15I	ATCGAT	1 cut(s)	2095
BsuI	GTATCC	1 cut(s)	2361
BsuRI	GGCC	5 cut(s)	156, 165, 1447, 1690, 1778
BsuTUI	ATCGAT	1 cut(s)	2095
BtgI	CCRYGG	2 cut(s)	34, 160
BtgZI	GCGATG	1 cut(s)	121
BtrI	CACGTC	2 cut(s)	445, 2086
BtsCI	GGATG	3 cut(s)	828, 1618, 2383
BtsIMutI	CAGTG	4 cut(s)	11, 300, 1862, 2344
BveI	ACCTGC	1 cut(s)	1425
Cac8I	GCNNGC	7 cut(s)	133, 325, 363, 372, 495, 555, 968
CaiI	CAGNNNCTG	1 cut(s)	2506
CfoI	GCGC	5 cut(s)	323, 370, 956, 1122, 1973
Cfr10I	RCCGGY	3 cut(s)	323, 370, 553
Cfr13I	GGNCC	5 cut(s)	154, 793, 806, 856, 1776
ClaI	ATCGAT	1 cut(s)	2095
CseI	GACGC	1 cut(s)	510
Csp6I	GTAC	5 cut(s)	412, 889, 2185, 2196, 2360
CviAII	CATG	9 cut(s)	35, 52, 242, 457, 1087, 1210, 1732, 1759, 2554
CviQI	GTAC	5 cut(s)	412, 889, 2185, 2196, 2360
DdeI	CTNAG	8 cut(s)	142, 932, 977, 1626, 1788, 1995, 2159, 2208
DinI	GGCGCC	1 cut(s)	955
DraI	TTTAAA	1 cut(s)	1752
DraIII	CACNNNGTG	1 cut(s)	1906
DrdI	GACNNNNNNGTC	1 cut(s)	2543
DriI	GACNNNNNGTC	1 cut(s)	1761
DseDI	GACNNNNNNGTC	1 cut(s)	2543
EaeI	YGGCCR	1 cut(s)	163
EagI	CGGCCG	1 cut(s)	163
Eam1104I	CTCTTC	6 cut(s)	864, 1421, 1532, 1734, 2458, 2475
Eam1105I	GACNNNNNGTC	1 cut(s)	1761
EarI	CTCTTC	6 cut(s)	864, 1421, 1532, 1734, 2458, 2475
EciI	GGCGGA	3 cut(s)	378, 512, 675
Ecl136II	GAGCTC	3 cut(s)	100, 532, 568
EclXI	CGGCCG	1 cut(s)	163
Eco130I	CCWWGG	2 cut(s)	34, 957
Eco24I	GRGCYC	4 cut(s)	102, 534, 570, 2470
Eco47I	GGWCC	3 cut(s)	793, 806, 856
Eco52I	CGGCCG	1 cut(s)	163
Eco53kI	GAGCTC	3 cut(s)	100, 532, 568
Eco57I	CTGAAG	1 cut(s)	35
Eco72I	CACGTG	1 cut(s)	1903
Eco88I	CYCGRG	2 cut(s)	433, 572
EcoICRI	GAGCTC	3 cut(s)	100, 532, 568
EcoO109I	RGGNCCY	3 cut(s)	793, 856, 1776
EcoRII	CCWGG	5 cut(s)	126, 516, 1317, 1690, 2499
EcoT14I	CCWWGG	2 cut(s)	34, 957
EcoT22I	ATGCAT	3 cut(s)	913, 1340, 2410
EcoT38I	GRGCYC	4 cut(s)	102, 534, 570, 2470
EgeI	GGCGCC	1 cut(s)	955
EheI	GGCGCC	1 cut(s)	955
ErhI	CCWWGG	2 cut(s)	34, 957
FaeI	CATG	9 cut(s)	38, 55, 245, 460, 1090, 1213, 1735, 1762, 2557
FalI	AAGNNNNNCTT	6 cut(s)	1260, 1292, 1554, 1586, 2417, 2449
FaqI	GGGAC	4 cut(s)	262, 290, 779, 794
FatI	CATG	9 cut(s)	34, 51, 241, 456, 1086, 1209, 1731, 1758, 2553
FauI	CCCGC	8 cut(s)	239, 252, 316, 323, 519, 774, 1046, 1382
FbaI	TGATCA	3 cut(s)	115, 945, 1489
FokI	GGATG	3 cut(s)	835, 1625, 2390
FriOI	GRGCYC	4 cut(s)	102, 534, 570, 2470
FspBI	CTAG	3 cut(s)	753, 1253, 1712
FspI	TGCGCA	1 cut(s)	1121
GlaI	GCGC	5 cut(s)	322, 369, 955, 1121, 1972
GsaI	CCCAGC	1 cut(s)	781
HaeII	RGCGCY	3 cut(s)	324, 957, 1974
HaeIII	GGCC	5 cut(s)	156, 165, 1447, 1690, 1778
HapII	CCGG	8 cut(s)	69, 152, 324, 371, 554, 1610, 2237, 2282
HgaI	GACGC	1 cut(s)	510
HhaI	GCGC	5 cut(s)	323, 370, 956, 1122, 1973
Hin1I	GRCGYC	3 cut(s)	521, 634, 954
Hin1II	CATG	9 cut(s)	38, 55, 245, 460, 1090, 1213, 1735, 1762, 2557
Hin6I	GCGC	5 cut(s)	321, 368, 954, 1120, 1971
HinP1I	GCGC	5 cut(s)	321, 368, 954, 1120, 1971
HincII	GTYRAC	2 cut(s)	622, 1828
HindII	GTYRAC	2 cut(s)	622, 1828
HpaII	CCGG	8 cut(s)	69, 152, 324, 371, 554, 1610, 2237, 2282
HphI	GGTGA	5 cut(s)	110, 1087, 1474, 1897, 1957
Hpy166II	GTNNAC	5 cut(s)	622, 1828, 1888, 1909, 2185
Hpy8I	GTNNAC	5 cut(s)	622, 1828, 1888, 1909, 2185
Hpy99I	CGWCG	9 cut(s)	191, 296, 446, 526, 629, 636, 639, 845, 854
HpyAV	CCTTC	6 cut(s)	1124, 1149, 1216, 1766, 2102, 2368
HpyCH4III	ACNGT	6 cut(s)	304, 561, 1172, 2041, 2152, 2195
HpyCH4IV	ACGT	6 cut(s)	225, 444, 634, 1902, 1911, 2085
HpyF3I	CTNAG	8 cut(s)	142, 932, 977, 1626, 1788, 1995, 2159, 2208
HpySE526I	ACGT	6 cut(s)	225, 444, 634, 1902, 1911, 2085
Hsp92I	GRCGYC	3 cut(s)	521, 634, 954
Hsp92II	CATG	9 cut(s)	38, 55, 245, 460, 1090, 1213, 1735, 1762, 2557
HspAI	GCGC	5 cut(s)	321, 368, 954, 1120, 1971
KasI	GGCGCC	1 cut(s)	953
KflI	GGGWCCC	1 cut(s)	793
Kpn2I	TCCGGA	2 cut(s)	1609, 2236
KroI	GCCGGC	3 cut(s)	323, 370, 553
KroNI	GCCGGC	3 cut(s)	325, 372, 555
Ksp22I	TGATCA	3 cut(s)	115, 945, 1489
LguI	GCTCTTC	1 cut(s)	2458
Lsp1109I	GCAGC	7 cut(s)	339, 352, 1390, 1462, 1642, 1670, 2514
LweI	GCATC	6 cut(s)	144, 898, 1006, 1576, 2061, 2239
MaeI	CTAG	3 cut(s)	753, 1253, 1712
MaeII	ACGT	6 cut(s)	225, 444, 634, 1902, 1911, 2085
MaeIII	GTNAC	5 cut(s)	653, 1261, 1963, 2024, 2321
MflI	RGATCY	4 cut(s)	60, 385, 874, 2493
MhlI	GDGCHC	8 cut(s)	102, 302, 534, 570, 683, 1852, 2346, 2470
Mly113I	GGCGCC	1 cut(s)	954
MlyI	GAGTC	4 cut(s)	605, 910, 1771, 1780
MmeI	TCCRAC	2 cut(s)	432, 543
Mph1103I	ATGCAT	3 cut(s)	913, 1340, 2410
MreI	CGCCGGCG	1 cut(s)	370
MroI	TCCGGA	2 cut(s)	1609, 2236
MroNI	GCCGGC	3 cut(s)	323, 370, 553
MroXI	GAANNNNTTC	1 cut(s)	23
MseI	TTAA	3 cut(s)	417, 1310, 1751
MspA1I	CMGCKG	3 cut(s)	330, 493, 1883
MspCI	CTTAAG	1 cut(s)	416
MspI	CCGG	8 cut(s)	69, 152, 324, 371, 554, 1610, 2237, 2282
MspR9I	CCNGG	6 cut(s)	128, 153, 518, 1319, 1692, 2501
Mva1269I	GAATGC	1 cut(s)	1338
MvaI	CCWGG	5 cut(s)	128, 518, 1319, 1692, 2501
MvnI	CGCG	1 cut(s)	526
NaeI	GCCGGC	3 cut(s)	325, 372, 555
NarI	GGCGCC	1 cut(s)	954
NciI	CCSGG	1 cut(s)	153
NcoI	CCATGG	1 cut(s)	34
NgoMIV	GCCGGC	3 cut(s)	323, 370, 553
NlaIII	CATG	9 cut(s)	38, 55, 245, 460, 1090, 1213, 1735, 1762, 2557
NlaIV	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 858, 955, 1777
NmeAIII	GCCGAG	2 cut(s)	191, 1666
NmuCI	GTSAC	2 cut(s)	1261, 1963
NsbI	TGCGCA	1 cut(s)	1121
NsiI	ATGCAT	3 cut(s)	913, 1340, 2410
PciSI	GCTCTTC	1 cut(s)	2458
PcsI	WCGNNNNNNNCGW	5 cut(s)	183, 192, 663, 849, 1908
PctI	GAATGC	1 cut(s)	1338
PdiI	GCCGGC	3 cut(s)	325, 372, 555
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	8 cut(s)	106, 146, 197, 263, 587, 1353, 1598, 1794
PflFI	GACNNNGTC	1 cut(s)	748
PflMI	CCANNNNNTGG	2 cut(s)	2443, 2506
PfoI	TCCNGGA	1 cut(s)	2499
PleI	GAGTC	4 cut(s)	605, 910, 1770, 1780
PluTI	GGCGCC	1 cut(s)	957
PmaCI	CACGTG	1 cut(s)	1903
PmlI	CACGTG	1 cut(s)	1903
PpsI	GAGTC	4 cut(s)	605, 910, 1770, 1780
Ppu21I	YACGTR	1 cut(s)	1903
PpuMI	RGGWCCY	2 cut(s)	793, 856
Psp124BI	GAGCTC	3 cut(s)	102, 534, 570
Psp5II	RGGWCCY	2 cut(s)	793, 856
Psp6I	CCWGG	5 cut(s)	126, 516, 1317, 1690, 2499
PspCI	CACGTG	1 cut(s)	1903
PspFI	CCCAGC	1 cut(s)	777
PspGI	CCWGG	5 cut(s)	126, 516, 1317, 1690, 2499
PspN4I	GGNNCC	9 cut(s)	387, 501, 664, 794, 795, 807, 858, 955, 1777
PspPI	GGNCC	5 cut(s)	154, 793, 806, 856, 1776
PspPPI	RGGWCCY	2 cut(s)	793, 856
PstI	CTGCAG	1 cut(s)	1660
PstNI	CAGNNNCTG	1 cut(s)	2506
PsuI	RGATCY	4 cut(s)	60, 385, 874, 2493
PsyI	GACNNNGTC	1 cut(s)	748
RsaI	GTAC	5 cut(s)	413, 890, 2186, 2197, 2361
RsaNI	GTAC	5 cut(s)	412, 889, 2185, 2196, 2360
SacI	GAGCTC	3 cut(s)	102, 534, 570
SapI	GCTCTTC	1 cut(s)	2458
SaqAI	TTAA	3 cut(s)	417, 1310, 1751
Sau96I	GGNCC	5 cut(s)	154, 793, 806, 856, 1776
SchI	GAGTC	4 cut(s)	605, 910, 1771, 1780
ScrFI	CCNGG	6 cut(s)	128, 153, 518, 1319, 1692, 2501
SduI	GDGCHC	8 cut(s)	102, 302, 534, 570, 683, 1852, 2346, 2470
SfaNI	GCATC	6 cut(s)	144, 898, 1006, 1576, 2061, 2239
SfcI	CTRYAG	2 cut(s)	1656, 2148
SfiI	GGCCNNNNNGGCC	1 cut(s)	162
SfoI	GGCGCC	1 cut(s)	955
SgrAI	CRCCGGYG	1 cut(s)	370
SinI	GGWCC	3 cut(s)	793, 806, 856
SmlI	CTYRAG	3 cut(s)	416, 533, 2355
SmoI	CTYRAG	3 cut(s)	416, 533, 2355
SspDI	GGCGCC	1 cut(s)	953
SspMI	CTAG	3 cut(s)	753, 1253, 1712
SstI	GAGCTC	3 cut(s)	102, 534, 570
StyD4I	CCNGG	6 cut(s)	126, 151, 516, 1317, 1690, 2499
StyI	CCWWGG	2 cut(s)	34, 957
TaaI	ACNGT	6 cut(s)	304, 561, 1172, 2041, 2152, 2195
TaiI	ACGT	6 cut(s)	228, 447, 637, 1905, 1914, 2088
TaqI	TCGA	8 cut(s)	41, 186, 266, 395, 1046, 1581, 1897, 2095
TatI	WGTACW	2 cut(s)	888, 2359
TauI	GCSGC	5 cut(s)	377, 441, 493, 581, 840
TfiI	GAWTC	8 cut(s)	106, 146, 197, 263, 587, 1353, 1598, 1794
Tru1I	TTAA	3 cut(s)	417, 1310, 1751
Tru9I	TTAA	3 cut(s)	417, 1310, 1751
TscAI	CASTG	4 cut(s)	18, 307, 1869, 2351
TseFI	GTSAC	2 cut(s)	1261, 1963
TseI	GCWGC	7 cut(s)	327, 365, 1403, 1475, 1655, 1658, 2527
Tsp45I	GTSAC	2 cut(s)	1261, 1963
TspGWI	ACGGA	3 cut(s)	299, 1858, 2537
TspRI	CASTG	4 cut(s)	18, 307, 1869, 2351
Tth111I	GACNNNGTC	1 cut(s)	748
Van91I	CCANNNNNTGG	2 cut(s)	2443, 2506
Vha464I	CTTAAG	1 cut(s)	416
VpaK11BI	GGWCC	3 cut(s)	793, 806, 856
XapI	RAATTY	4 cut(s)	1108, 1590, 1804, 2590
XbaI	TCTAGA	1 cut(s)	752
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	3 cut(s)	753, 1253, 1712
ZraI	GACGTC	1 cut(s)	635
Zsp2I	ATGCAT	3 cut(s)	913, 1340, 2410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.