Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0001376

Physical Location & Seq

Genomic Coordinates Reverse (-)

13825406 .. 13830140

Gene Structure 4735 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g15780.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGGATTTCTCACTCAGGAAATCCTTCAAATCTCTCGCCTCCGAAAAGCACTTGCGAAACGAAGCACAAGAGAAGCTTCCCATTTTGTTGGACCACCACAACGAACAGCATCAAGCCATGAGCGCCGCCGCCGATTCTTCCTCCGATCGCCGCGAGGTCATCGTCAAGATCGACGAGGGCGGCGGCTCCGGTGCCGGTAAGGAAGCGGAAACGGCCAAGCAGGGTGGCGGGATTTGGCGGGAGTCGAGCATTGATTTCTGGAAGGACGACGAGAGCAATGGCGGCGGTTTTGAGTTTGAGAAATCGGCCGCGAAGAAGACGGAGGAGAAGAAGGAGGAGGATCCGCCGTCGAAGCTGATAGGGCAGTTCCTGCACAAGCAGAGAGCCTCCGGCGACTTGTCGCTGGACATGGATTTGGAGATGGACGAGCTGCGGCCGCATGACTTGCCGCCGGTGGCGGAGTCTCCGAGGAACTCCCGGACGTCGAGGGAGATCAAGGTGTCGTTCCAGTCGCCGGACGCCGAGTCCGAGTCGGTGAGGCGGCGCCACAAGGACTCTGCTGACGAGGAGCGCCGTCGTAGCAGCCGGCTCAGTAACGGCGGTGCTGACGTCATCAGGTGCACGTCGAACGCGTCATTCGGGCGAGAGCCGTCGTTTCAGAACAGGTCTGATTTGCTGAGGCTGAAGACGAAGTCGAGACTGATGGACCCGCCCGAGGGGTGGGACGAGGGGCCGGGTCGGGTCCCGAGATCGGGTCTGATGAGGTCCGGGATGTTGGGCCGGGGCGGAGGGGAGGATGAGGAGGATGACCCGTTTCTGGAAGAGGACTTGCCTGATGAGTACAAGAGGGCTAACTTCAATGCATTGACTCTGGTCCAGTGGCTGAGTTTGGTTTTGATTATTGGGGCATTGGCTTGTACATTGTCTATTAAAGTTTTGAGGGAGAAGAATCTGTGGAAATTGAAGATGTGGAAATGGGAGGTGCTGATTTTGGTTTCGATTTGTGGGAGGCTGGTTTCGGGTTGGATGATAAGGCTTATGGTGTTCTTTGTGGAGAGGAATTTCATTTTGAGGAAGAGGGTGTTGTATTTTGTGTATGGGATCAGGAAGGCTGTTCAGAACTGTATTTGGCTTGGCCTCGTTTTGATGGGTTGGCATTTCTTGCTGATCAGGAAGGTTGAGAAGGAAACCAATACTAGAATCCTTGAGTATGTGAGTAAAGTGTTGCTTTGTCTCTTGATTGGGGGTTTGATTTGGTTGGTGAAGACCTTGGTTGTGAAGGTGCTTGCTTCGTCTTTCCATGTTAGATCGTATTTTGATCGGATTCAGGATGCGCTTTTTAATCAGTATGTGATCCAAACATTGTCTGGGCGGCCGTGGATTGAGATACAGAAAGGGGAGGATGAAGAGGAGAGGCTTGCTGACGAGGTTAGGAAGCTACAGAATGCTGGAGCTACTATGCCTCCTGACCTCAAAGCAAATGCGTTGCCCCCGTCTGGGAATATTGGGAAGCCGGTTGGCAATGGCTCTGGGAGAAGTGGGCGGTTGATTGCTGCAAGTGGAGGGCTTGTGGGGAAGAGTGGCAATGTTTCAAAGCCACTTTCCAAGAAGTCAGAGGAGGTCGGGATCACAATTGGTCACCTGCATAGGCTCAATCCAAAGAATGTGTCTGCTTGGAATATGAAGAGGCTCATGAATATAGTTAGAAATGGGCATCTTACCACACTGGATGAGCAGATTCTGGATTCCACCAAGGATGAGGGTGATACTCAGATCAGAAGTGAAGTTGAGGCAAAGGCTGCCGCTAAAAAGATATTTTGCAATGTGGCTAGGCCTGGCTCCAAGCACATCTACCTGGAGGATTTGTTGCGTTTCATGGAAGAAGATGTGGCTTTAAAGACCATGAGTCTCTTTGAAGGGGCCTCTGAGAGTAAGAGAATCAGCAAAACTTTACTGAAAAATTGGGTGGTCAATTGCGTTAGAGAACGGAGAGCACTTGCTTTGACACTGAATGACACCAAAACAGCAGTGAACAGACTTCGACGCGTGGTGGACGTATTAGTTAGCATTGTTATTATAGTTATATGGCTGCTTGTGCTGGGCATCGTCACTAGTGCCAATATTGTTTATGCTACTTCTCAACTTGTGATTGTGGCATTTATTTTTGGTAACTCATGCAAGACAGTCTTTGAAGCAGTCATATTTTTATTTGTGATGCACCCTTTTGACGTGGGAGATCGATGTGAAATTGAAGGGGTTCAGATGGTTGTAGAGGAAATGAATATCTTGACTACAGTTTTTCTGAGGTATGACAACCTGAAGATTGTGTACCCAAACAGTACCCTTTCAACCAAGCCTATCAATAACTTTTATCGAAGTCCGGACACCGGAGATGCTGTTGAGTTTTGTGTTCATATAACTACACCGGCAGACAAGATAGCTGTTATGAAGCAGAGAATGATAAGTTACATTGAGAATAAGAAGGAACACTGGCATCCTTCACCAATGATCATATTGAAGGAAGTAGAAGAATTAAATAGGATGAGGTTTGCTGTGTGGCTGACACATAGAATGAACTTCCAAGACATGGGAGAAAAGTGGGCAAGGAGAGCCCTTTTACTTGAAGAGATGGTACGGGTCTTCCAGGAGCTTGATATTCAGTACCGTCTACTGCCTATTGACATAAACGTCCGCGGTATGCCTCCCACAAGCACCGGTAACTTCACAACAACCACAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006820 GO:0008150 GO:0008381 GO:0009506 GO:0015267 GO:0016020 GO:0022803 GO:0022836 GO:0022857 GO:0030054 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MS_channel

Protein Analysis

914

Amino Acids

103.39

Weight (kDa)

8.78

Isoelectric Point (pI)

49.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MS_channel_2nd	PF00924	734 - 790	1.5e-10	Mechanosensitive ion channel, beta-domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001376)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78610 AT2G17010 AT2G17010
fragaria_vesca	FvH4_2g15780 FvH4_2g37870 FvH4_2g37870 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430 FvH4_3g39430
malus_domestica	MD05G1126700.v1.1 MD08G1006800.v1.1 MD08G1006900.v1.1 MD10G1129900.v1.1
prunus_persica	Prupe.1G358700_v2.0.a1 Prupe.8G170400_v2.0.a1
pyrus_communis	pycom05g12260 pycom08g00530 pycom08g00560 pycom08g00580
rosa_chinensis	RchiOBHm_Chr5g0071021 RchiOBHm_Chr6g0279131 RchiOBHm_Chr6g0303751
rosa_laevigata	RLG00000011049 RLG00000013165 RLG00000036184
rosa_multiflora	Rmu_co8207138.1_g000001 Rmu_co8395065.1_g000001 Rmu_sc0001141.1_g000023 Rmu_sc0012878.1_g000006 Rmu_sc0014203.1_g000020
rosa_roxburghii	Rroxscaffold_1G00010020 Rroxscaffold_7G00164430 Rroxscaffold_7G00189100
rosa_rugosa	Rorug05G0409800 Rorug05G0409900 Rorug05G0410000 Rorug06G0122500 Rorug06G0324500
rosa_samantha	Rh5AG466100 Rh5BG484300 Rh5CG508900 Rh5DG495700 Rh6AG231300 Rh6AG438800 Rh6BG236200 Rh6BG464300 Rh6CG238200 Rh6CG451100 Rh6DG229800 Rh6DG438000
rosa_wichuraiana	Rw5G043280 Rw6G037990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1650
AasI	GACNNNNNNGTC	1 cut(s)	2687
AatII	GACGTC	2 cut(s)	485, 612
Acc36I	ACCTGC	1 cut(s)	1650
AccB1I	GGYRCC	2 cut(s)	191, 543
AccB7I	CCANNNNNTGG	1 cut(s)	2587
AccI	GTMKAC	1 cut(s)	2668
AccII	CGCG	5 cut(s)	153, 311, 632, 2046, 2694
AccIII	TCCGGA	1 cut(s)	2380
AclWI	GGATC	5 cut(s)	335, 348, 1109, 1348, 1634
AcoI	YGGCCR	4 cut(s)	213, 306, 434, 1373
AcsI	RAATTY	1 cut(s)	1060
AcuI	CTGAAG	2 cut(s)	704, 2339
AcyI	GRCGYC	4 cut(s)	482, 519, 544, 609
AfaI	GTAC	6 cut(s)	842, 919, 2330, 2341, 2634, 2663
AflIII	ACRYGT	2 cut(s)	630, 2044
AgeI	ACCGGT	1 cut(s)	2714
AhdI	GACNNNNNGTC	2 cut(s)	523, 1905
AhlI	ACTAGT	1 cut(s)	2111
AjiI	CACGTC	2 cut(s)	624, 2230
AjnI	CCWGG	3 cut(s)	1834, 1854, 2643
AluBI	AGCT	8 cut(s)	76, 355, 430, 1438, 1454, 2441, 2650, 2739
AluI	AGCT	8 cut(s)	76, 355, 430, 1438, 1454, 2441, 2650, 2739
Alw21I	GWGCWC	2 cut(s)	623, 1996
Alw26I	GTCTC	4 cut(s)	468, 691, 1238, 1913
Alw44I	GTGCAC	1 cut(s)	619
AlwI	GGATC	5 cut(s)	335, 348, 1109, 1348, 1634
AlwNI	CAGNNNCTG	3 cut(s)	370, 883, 1741
Ama87I	CYCGRG	2 cut(s)	713, 745
Aor13HI	TCCGGA	1 cut(s)	2380
AoxI	GGCC	9 cut(s)	213, 306, 434, 731, 778, 1135, 1373, 1832, 1920
ApaLI	GTGCAC	1 cut(s)	619
ApeKI	GCWGC	5 cut(s)	430, 582, 1553, 1799, 2089
ApoI	RAATTY	1 cut(s)	1060
ArsI	GACNNNNNNTTYG	2 cut(s)	1652, 1684
AsiGI	ACCGGT	1 cut(s)	2714
Asp700I	GAANNNNTTC	2 cut(s)	23, 2256
AspLEI	GCGC	4 cut(s)	125, 546, 573, 1336
AspS9I	GGNCC	8 cut(s)	91, 706, 731, 742, 765, 778, 874, 1920
AsuC2I	CCSGG	4 cut(s)	478, 735, 769, 782
AsuHPI	GGTGA	5 cut(s)	547, 1273, 1631, 1775, 2493
AvaI	CYCGRG	2 cut(s)	713, 745
AvaII	GGWCC	5 cut(s)	91, 706, 742, 765, 874
BaeGI	GKGCMC	1 cut(s)	623
BamHI	GGATCC	1 cut(s)	340
BanI	GGYRCC	2 cut(s)	191, 543
BanII	GRGCYC	1 cut(s)	2614
BarI	GAAGNNNNNNTAC	4 cut(s)	2312, 2344, 2616, 2648
BbsI	GAAGAC	4 cut(s)	323, 692, 1271, 2632
Bbv12I	GWGCWC	2 cut(s)	623, 1996
BbvCI	CCTCAGC	1 cut(s)	677
BbvI	GCAGC	5 cut(s)	417, 594, 1540, 1786, 2076
BccI	CCATC	5 cut(s)	415, 697, 1141, 2257, 2623
BceAI	ACGGC	6 cut(s)	228, 331, 558, 613, 634, 1360
BciT130I	CCWGG	3 cut(s)	1836, 1856, 2645
BclI	TGATCA	2 cut(s)	1167, 2508
BcnI	CCSGG	4 cut(s)	478, 735, 769, 782
BcoDI	GTCTC	4 cut(s)	468, 691, 1238, 1913
BcuI	ACTAGT	1 cut(s)	2111
BfaI	CTAG	3 cut(s)	1197, 1830, 2112
BfmI	CTRYAG	2 cut(s)	1439, 2292
BfoI	RGCGCY	3 cut(s)	126, 547, 574
BfuAI	ACCTGC	1 cut(s)	1650
Bme1390I	CCNGG	7 cut(s)	478, 735, 769, 782, 1836, 1856, 2645
Bme18I	GGWCC	5 cut(s)	91, 706, 742, 765, 874
BmeRI	GACNNNNNGTC	2 cut(s)	523, 1905
BmeT110I	CYCGRG	2 cut(s)	713, 745
BmgBI	CACGTC	2 cut(s)	624, 2230
BmgT120I	GGNCC	8 cut(s)	91, 706, 731, 742, 765, 778, 874, 1920
BmrFI	CCNGG	7 cut(s)	478, 735, 769, 782, 1836, 1856, 2645
BmsI	GCATC	7 cut(s)	118, 1321, 1723, 2112, 2205, 2383, 2503
BoxI	GACNNNNGTC	1 cut(s)	872
BpiI	GAAGAC	4 cut(s)	323, 692, 1271, 2632
BpmI	CTGGAG	2 cut(s)	1470, 1877
Bpu10I	CCTNAGC	1 cut(s)	677
BpuEI	CTTGAG	1 cut(s)	1226
BpuMI	CCSGG	4 cut(s)	478, 735, 769, 782
Bsa29I	ATCGAT	1 cut(s)	2239
BsaHI	GRCGYC	4 cut(s)	482, 519, 544, 609
BsaJI	CCNNGG	7 cut(s)	467, 714, 781, 1269, 1376, 1752, 2692
BsaWI	WCCGGW	4 cut(s)	188, 2380, 2387, 2714
BsaXI	ACNNNNNCTCC	8 cut(s)	1447, 1477, 1554, 1584, 1850, 1880, 2382, 2412
Bse118I	RCCGGY	6 cut(s)	194, 451, 585, 1513, 2425, 2714
Bse1I	ACTGG	4 cut(s)	508, 877, 1731, 2495
Bse3DI	GCAATG	4 cut(s)	283, 1528, 1591, 1828
BseAI	TCCGGA	1 cut(s)	2380
BseBI	CCWGG	3 cut(s)	1836, 1856, 2645
BseCI	ATCGAT	1 cut(s)	2239
BseDI	CCNNGG	7 cut(s)	467, 714, 781, 1269, 1376, 1752, 2692
BseMI	GCAATG	4 cut(s)	283, 1528, 1591, 1828
BseMII	CTCAG	7 cut(s)	28, 604, 668, 875, 1784, 1917, 2294
BseNI	ACTGG	4 cut(s)	508, 877, 1731, 2495
BseRI	GAGGAG	6 cut(s)	338, 350, 581, 815, 1424, 1631
BseSI	GKGCMC	1 cut(s)	623
BseX3I	CGGCCG	3 cut(s)	306, 434, 1373
BseXI	GCAGC	5 cut(s)	417, 594, 1540, 1786, 2076
BseYI	CCCAGC	1 cut(s)	2098
BsgI	GTGCAG	1 cut(s)	356
Bsh1236I	CGCG	5 cut(s)	153, 311, 632, 2046, 2694
Bsh1285I	CGRYCG	4 cut(s)	148, 309, 437, 1376
BshFI	GGCC	9 cut(s)	215, 308, 436, 733, 780, 1137, 1375, 1834, 1922
BshNI	GGYRCC	2 cut(s)	191, 543
BshTI	ACCGGT	1 cut(s)	2714
BshVI	ATCGAT	1 cut(s)	2239
BsiEI	CGRYCG	4 cut(s)	148, 309, 437, 1376
BsiHKAI	GWGCWC	2 cut(s)	623, 1996
BsiHKCI	CYCGRG	2 cut(s)	713, 745
BslFI	GGGAC	2 cut(s)	728, 737
BsmAI	GTCTC	4 cut(s)	468, 691, 1238, 1913
BsmFI	GGGAC	2 cut(s)	728, 737
BsmI	GAATGC	1 cut(s)	1450
BsnI	GGCC	9 cut(s)	215, 308, 436, 733, 780, 1137, 1375, 1834, 1922
BsoBI	CYCGRG	2 cut(s)	713, 745
Bsp1286I	GDGCHC	3 cut(s)	623, 1996, 2614
Bsp13I	TCCGGA	1 cut(s)	2380
Bsp1407I	TGTACA	1 cut(s)	917
BspANI	GGCC	9 cut(s)	215, 308, 436, 733, 780, 1137, 1375, 1834, 1922
BspCNI	CTCAG	7 cut(s)	27, 603, 669, 876, 1783, 1918, 2295
BspDI	ATCGAT	1 cut(s)	2239
BspEI	TCCGGA	1 cut(s)	2380
BspFNI	CGCG	5 cut(s)	153, 311, 632, 2046, 2694
BspHI	TCATGA	1 cut(s)	1692
BspMI	ACCTGC	1 cut(s)	1650
BspPI	GGATC	5 cut(s)	335, 348, 1109, 1348, 1634
BspT107I	GGYRCC	2 cut(s)	191, 543
BsrDI	GCAATG	4 cut(s)	283, 1528, 1591, 1828
BsrFI	RCCGGY	6 cut(s)	194, 451, 585, 1513, 2425, 2714
BsrGI	TGTACA	1 cut(s)	917
BsrI	ACTGG	4 cut(s)	508, 877, 1731, 2495
BssAI	RCCGGY	6 cut(s)	194, 451, 585, 1513, 2425, 2714
BssECI	CCNNGG	7 cut(s)	467, 714, 781, 1269, 1376, 1752, 2692
BssNI	GRCGYC	4 cut(s)	482, 519, 544, 609
BssT1I	CCWWGG	2 cut(s)	1269, 1752
Bst2UI	CCWGG	3 cut(s)	1836, 1856, 2645
Bst4CI	ACNGT	5 cut(s)	1124, 2185, 2296, 2339, 2666
Bst6I	CTCTTC	6 cut(s)	816, 1070, 1401, 1571, 1679, 2619
BstACI	GRCGYC	4 cut(s)	482, 519, 544, 609
BstAPI	GCANNNNNTGC	4 cut(s)	370, 445, 1162, 1799
BstAUI	TGTACA	1 cut(s)	917
BstC8I	GCNNGC	3 cut(s)	587, 1287, 1419
BstDEI	CTNAG	7 cut(s)	14, 590, 677, 884, 1770, 1926, 2303
BstDSI	CCRYGG	2 cut(s)	1376, 2692
BstEII	GGTNACC	1 cut(s)	1637
BstFNI	CGCG	5 cut(s)	153, 311, 632, 2046, 2694
BstH2I	RGCGCY	3 cut(s)	126, 547, 574
BstHHI	GCGC	4 cut(s)	125, 546, 573, 1336
BstMAI	GTCTC	4 cut(s)	468, 691, 1238, 1913
BstMCI	CGRYCG	4 cut(s)	148, 309, 437, 1376
BstNI	CCWGG	3 cut(s)	1836, 1856, 2645
BstPAI	GACNNNNGTC	1 cut(s)	872
BstPI	GGTNACC	1 cut(s)	1637
BstSCI	CCNGG	7 cut(s)	476, 733, 767, 780, 1834, 1854, 2643
BstSFI	CTRYAG	2 cut(s)	1439, 2292
BstSLI	GKGCMC	1 cut(s)	623
BstUI	CGCG	5 cut(s)	153, 311, 632, 2046, 2694
BstV1I	GCAGC	5 cut(s)	417, 594, 1540, 1786, 2076
BstV2I	GAAGAC	4 cut(s)	323, 692, 1271, 2632
BstX2I	RGATCY	1 cut(s)	340
BstXI	CCANNNNNNTGG	1 cut(s)	88
BstYI	RGATCY	1 cut(s)	340
BstZI	CGGCCG	3 cut(s)	306, 434, 1373
Bsu15I	ATCGAT	1 cut(s)	2239
BsuRI	GGCC	9 cut(s)	215, 308, 436, 733, 780, 1137, 1375, 1834, 1922
BsuTUI	ATCGAT	1 cut(s)	2239
BtgI	CCRYGG	2 cut(s)	1376, 2692
BtrI	CACGTC	2 cut(s)	624, 2230
BtsI	GCAGTG	1 cut(s)	2034
BtsIMutI	CAGTG	5 cut(s)	884, 1724, 2006, 2034, 2488
BveI	ACCTGC	1 cut(s)	1650
Cac8I	GCNNGC	3 cut(s)	587, 1287, 1419
CaiI	CAGNNNCTG	3 cut(s)	370, 883, 1741
CciI	TCATGA	1 cut(s)	1692
CciNI	GCGGCCGC	1 cut(s)	434
CfoI	GCGC	4 cut(s)	125, 546, 573, 1336
Cfr10I	RCCGGY	6 cut(s)	194, 451, 585, 1513, 2425, 2714
Cfr13I	GGNCC	8 cut(s)	91, 706, 731, 742, 765, 778, 874, 1920
Cfr42I	CCGCGG	1 cut(s)	2695
ClaI	ATCGAT	1 cut(s)	2239
CseI	GACGC	3 cut(s)	527, 621, 2052
Csp6I	GTAC	6 cut(s)	841, 918, 2329, 2340, 2633, 2662
CspAI	ACCGGT	1 cut(s)	2714
CspCI	CAANNNNNGTGG	2 cut(s)	205, 240
CviAII	CATG	9 cut(s)	118, 409, 440, 1301, 1693, 1876, 1903, 2175, 2587
CviQI	GTAC	6 cut(s)	841, 918, 2329, 2340, 2633, 2662
DdeI	CTNAG	7 cut(s)	14, 590, 677, 884, 1770, 1926, 2303
DinI	GGCGCC	1 cut(s)	545
DraI	TTTAAA	1 cut(s)	1896
DrdI	GACNNNNNNGTC	1 cut(s)	2687
DriI	GACNNNNNGTC	2 cut(s)	523, 1905
DseDI	GACNNNNNNGTC	1 cut(s)	2687
EaeI	YGGCCR	4 cut(s)	213, 306, 434, 1373
EagI	CGGCCG	3 cut(s)	306, 434, 1373
Eam1104I	CTCTTC	6 cut(s)	816, 1070, 1401, 1571, 1679, 2619
Eam1105I	GACNNNNNGTC	2 cut(s)	523, 1905
EarI	CTCTTC	6 cut(s)	816, 1070, 1401, 1571, 1679, 2619
EciI	GGCGGA	3 cut(s)	333, 473, 801
EclXI	CGGCCG	3 cut(s)	306, 434, 1373
Eco130I	CCWWGG	2 cut(s)	1269, 1752
Eco147I	AGGCCT	1 cut(s)	1834
Eco24I	GRGCYC	1 cut(s)	2614
Eco47I	GGWCC	5 cut(s)	91, 706, 742, 765, 874
Eco52I	CGGCCG	3 cut(s)	306, 434, 1373
Eco57I	CTGAAG	2 cut(s)	704, 2339
Eco88I	CYCGRG	2 cut(s)	713, 745
Eco91I	GGTNACC	1 cut(s)	1637
EcoO109I	RGGNCCY	2 cut(s)	742, 1920
EcoO65I	GGTNACC	1 cut(s)	1637
EcoRII	CCWGG	3 cut(s)	1834, 1854, 2643
EcoT14I	CCWWGG	2 cut(s)	1269, 1752
EcoT22I	ATGCAT	1 cut(s)	865
EcoT38I	GRGCYC	1 cut(s)	2614
EgeI	GGCGCC	1 cut(s)	545
EheI	GGCGCC	1 cut(s)	545
ErhI	CCWWGG	2 cut(s)	1269, 1752
FaeI	CATG	9 cut(s)	121, 412, 443, 1304, 1696, 1879, 1906, 2178, 2590
FalI	AAGNNNNNCTT	6 cut(s)	60, 92, 1212, 1244, 2171, 2203
FaqI	GGGAC	2 cut(s)	728, 737
FatI	CATG	9 cut(s)	117, 408, 439, 1300, 1692, 1875, 1902, 2174, 2586
FauI	CCCGC	3 cut(s)	221, 231, 717
FbaI	TGATCA	2 cut(s)	1167, 2508
FblI	GTMKAC	1 cut(s)	2668
FriOI	GRGCYC	1 cut(s)	2614
FspBI	CTAG	3 cut(s)	1197, 1830, 2112
GlaI	GCGC	4 cut(s)	124, 545, 572, 1335
GsaI	CCCAGC	1 cut(s)	2102
GsuI	CTGGAG	2 cut(s)	1470, 1877
HaeII	RGCGCY	3 cut(s)	126, 547, 574
HaeIII	GGCC	9 cut(s)	215, 308, 436, 733, 780, 1137, 1375, 1834, 1922
HgaI	GACGC	3 cut(s)	527, 621, 2052
HhaI	GCGC	4 cut(s)	125, 546, 573, 1336
Hin1I	GRCGYC	4 cut(s)	482, 519, 544, 609
Hin1II	CATG	9 cut(s)	121, 412, 443, 1304, 1696, 1879, 1906, 2178, 2590
Hin6I	GCGC	4 cut(s)	123, 544, 571, 1334
HinP1I	GCGC	4 cut(s)	123, 544, 571, 1334
HindIII	AAGCTT	1 cut(s)	74
HphI	GGTGA	5 cut(s)	547, 1273, 1631, 1775, 2493
Hpy166II	GTNNAC	5 cut(s)	621, 2032, 2053, 2329, 2669
Hpy8I	GTNNAC	5 cut(s)	621, 2032, 2053, 2329, 2669
Hpy99I	CGWCG	8 cut(s)	176, 272, 352, 487, 579, 628, 655, 2046
HpyCH4III	ACNGT	5 cut(s)	1124, 2185, 2296, 2339, 2666
HpyCH4IV	ACGT	6 cut(s)	482, 609, 623, 2055, 2229, 2688
HpyCH4V	TGCA	8 cut(s)	373, 621, 863, 1556, 1645, 1821, 2178, 2218
HpyF3I	CTNAG	7 cut(s)	14, 590, 677, 884, 1770, 1926, 2303
HpySE526I	ACGT	6 cut(s)	482, 609, 623, 2055, 2229, 2688
Hsp92I	GRCGYC	4 cut(s)	482, 519, 544, 609
Hsp92II	CATG	9 cut(s)	121, 412, 443, 1304, 1696, 1879, 1906, 2178, 2590
HspAI	GCGC	4 cut(s)	123, 544, 571, 1334
KasI	GGCGCC	1 cut(s)	543
KflI	GGGWCCC	1 cut(s)	742
Kpn2I	TCCGGA	1 cut(s)	2380
KroI	GCCGGC	1 cut(s)	585
KroNI	GCCGGC	1 cut(s)	587
Ksp22I	TGATCA	2 cut(s)	1167, 2508
KspI	CCGCGG	1 cut(s)	2695
LmnI	GCTCC	5 cut(s)	191, 568, 1451, 1844, 2647
Lsp1109I	GCAGC	5 cut(s)	417, 594, 1540, 1786, 2076
LweI	GCATC	7 cut(s)	118, 1321, 1723, 2112, 2205, 2383, 2503
MaeI	CTAG	3 cut(s)	1197, 1830, 2112
MaeII	ACGT	6 cut(s)	482, 609, 623, 2055, 2229, 2688
MaeIII	GTNAC	6 cut(s)	593, 1637, 2107, 2168, 2465, 2717
MfeI	CAATTG	2 cut(s)	1632, 1972
MflI	RGATCY	1 cut(s)	340
MhlI	GDGCHC	3 cut(s)	623, 1996, 2614
MluCI	AATT	7 cut(s)	959, 1060, 1632, 1960, 1972, 2247, 2531
MluI	ACGCGT	2 cut(s)	630, 2044
Mly113I	GGCGCC	1 cut(s)	544
MlyI	GAGTC	7 cut(s)	251, 470, 533, 539, 548, 862, 1915
MmeI	TCCRAC	2 cut(s)	69, 1004
Mph1103I	ATGCAT	1 cut(s)	865
MroI	TCCGGA	1 cut(s)	2380
MroNI	GCCGGC	1 cut(s)	585
MroXI	GAANNNNTTC	2 cut(s)	23, 2256
MseI	TTAA	4 cut(s)	930, 1341, 1895, 2534
MspA1I	CMGCKG	1 cut(s)	2694
MspR9I	CCNGG	7 cut(s)	478, 735, 769, 782, 1836, 1856, 2645
MunI	CAATTG	2 cut(s)	1632, 1972
Mva1269I	GAATGC	1 cut(s)	1450
MvaI	CCWGG	3 cut(s)	1836, 1856, 2645
MvnI	CGCG	5 cut(s)	153, 311, 632, 2046, 2694
NaeI	GCCGGC	1 cut(s)	587
NarI	GGCGCC	1 cut(s)	544
NciI	CCSGG	4 cut(s)	478, 735, 769, 782
NgoMIV	GCCGGC	1 cut(s)	585
NlaIII	CATG	9 cut(s)	121, 412, 443, 1304, 1696, 1879, 1906, 2178, 2590
NmeAIII	GCCGAG	1 cut(s)	547
NmuCI	GTSAC	2 cut(s)	1637, 2107
NotI	GCGGCCGC	1 cut(s)	434
NsiI	ATGCAT	1 cut(s)	865
PagI	TCATGA	1 cut(s)	1692
PaqCI	CACCTGC	1 cut(s)	1650
PceI	AGGCCT	1 cut(s)	1834
PcsI	WCGNNNNNNNCGW	3 cut(s)	168, 525, 629
PctI	GAATGC	1 cut(s)	1450
PdiI	GCCGGC	1 cut(s)	587
PdmI	GAANNNNTTC	2 cut(s)	23, 2256
PfeI	GAWTC	7 cut(s)	134, 949, 1200, 1324, 1738, 1745, 1938
PflFI	GACNNNGTC	1 cut(s)	691
PflMI	CCANNNNNTGG	1 cut(s)	2587
PfoI	TCCNGGA	3 cut(s)	476, 767, 2643
PinAI	ACCGGT	1 cut(s)	2714
Ple19I	CGATCG	1 cut(s)	148
PleI	GAGTC	7 cut(s)	250, 469, 532, 538, 548, 862, 1914
PluTI	GGCGCC	1 cut(s)	547
PpsI	GAGTC	7 cut(s)	250, 469, 532, 538, 548, 862, 1914
PpuMI	RGGWCCY	1 cut(s)	742
PshAI	GACNNNNGTC	1 cut(s)	872
Psp5II	RGGWCCY	1 cut(s)	742
Psp6I	CCWGG	3 cut(s)	1834, 1854, 2643
PspEI	GGTNACC	1 cut(s)	1637
PspFI	CCCAGC	1 cut(s)	2098
PspGI	CCWGG	3 cut(s)	1834, 1854, 2643
PspPI	GGNCC	8 cut(s)	91, 706, 731, 742, 765, 778, 874, 1920
PspPPI	RGGWCCY	1 cut(s)	742
PstNI	CAGNNNCTG	3 cut(s)	370, 883, 1741
PsuI	RGATCY	1 cut(s)	340
PsyI	GACNNNGTC	1 cut(s)	691
PvuI	CGATCG	1 cut(s)	148
RsaI	GTAC	6 cut(s)	842, 919, 2330, 2341, 2634, 2663
RsaNI	GTAC	6 cut(s)	841, 918, 2329, 2340, 2633, 2662
SacII	CCGCGG	1 cut(s)	2695
SaqAI	TTAA	4 cut(s)	930, 1341, 1895, 2534
Sau96I	GGNCC	8 cut(s)	91, 706, 731, 742, 765, 778, 874, 1920
SchI	GAGTC	7 cut(s)	251, 470, 533, 539, 548, 862, 1915
ScrFI	CCNGG	7 cut(s)	478, 735, 769, 782, 1836, 1856, 2645
SduI	GDGCHC	3 cut(s)	623, 1996, 2614
SfaNI	GCATC	7 cut(s)	118, 1321, 1723, 2112, 2205, 2383, 2503
SfcI	CTRYAG	2 cut(s)	1439, 2292
SfoI	GGCGCC	1 cut(s)	545
Sfr303I	CCGCGG	1 cut(s)	2695
SgrAI	CRCCGGYG	1 cut(s)	451
SgrBI	CCGCGG	1 cut(s)	2695
SinI	GGWCC	5 cut(s)	91, 706, 742, 765, 874
SmlI	CTYRAG	1 cut(s)	1205
SmoI	CTYRAG	1 cut(s)	1205
SpeI	ACTAGT	1 cut(s)	2111
Sse9I	AATT	7 cut(s)	959, 1060, 1632, 1960, 1972, 2247, 2531
SseBI	AGGCCT	1 cut(s)	1834
SspDI	GGCGCC	1 cut(s)	543
SspI	AATATT	2 cut(s)	1504, 2122
SspMI	CTAG	3 cut(s)	1197, 1830, 2112
StuI	AGGCCT	1 cut(s)	1834
StyD4I	CCNGG	7 cut(s)	476, 733, 767, 780, 1834, 1854, 2643
StyI	CCWWGG	2 cut(s)	1269, 1752
TaaI	ACNGT	5 cut(s)	1124, 2185, 2296, 2339, 2666
TaiI	ACGT	6 cut(s)	485, 612, 626, 2058, 2232, 2691
TasI	AATT	7 cut(s)	959, 1060, 1632, 1960, 1972, 2247, 2531
TatI	WGTACW	2 cut(s)	840, 917
TfiI	GAWTC	7 cut(s)	134, 949, 1200, 1324, 1738, 1745, 1938
Tru1I	TTAA	4 cut(s)	930, 1341, 1895, 2534
Tru9I	TTAA	4 cut(s)	930, 1341, 1895, 2534
TscAI	CASTG	5 cut(s)	884, 1731, 2013, 2034, 2495
TseFI	GTSAC	2 cut(s)	1637, 2107
TseI	GCWGC	5 cut(s)	430, 582, 1553, 1799, 2089
Tsp45I	GTSAC	2 cut(s)	1637, 2107
TspDTI	ATGAA	9 cut(s)	1054, 1419, 1697, 1709, 1864, 2294, 2402, 2462, 2588
TspGWI	ACGGA	2 cut(s)	335, 2002
TspRI	CASTG	5 cut(s)	884, 1731, 2013, 2034, 2495
Tth111I	GACNNNGTC	1 cut(s)	691
Van91I	CCANNNNNTGG	1 cut(s)	2587
VneI	GTGCAC	1 cut(s)	619
VpaK11BI	GGWCC	5 cut(s)	91, 706, 742, 765, 874
XapI	RAATTY	1 cut(s)	1060
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1364
XmiI	GTMKAC	1 cut(s)	2668
XmnI	GAANNNNTTC	2 cut(s)	23, 2256
XspI	CTAG	3 cut(s)	1197, 1830, 2112
ZraI	GACGTC	2 cut(s)	483, 610
Zsp2I	ATGCAT	1 cut(s)	865

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.