Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

8989275 .. 8993275

Gene Structure 4001 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G20900.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGGAGAAATACAACAGTTTATCAGATTTTCTCAAGGAGTTCTACATCCCTACGTACGTCCTCTCGGCGGAAACAGAAGAGGAGGAGGAGGAGGAGAGTCGTCCTACACCTGCGAGCCCCGTCCTTGTCTTCATCAACTCCAAAAGCGGTGGTCAGTTGGGTGGCGAACTCATTCTCACCTACCGATCTCTTCTCAATCACAATCAGGTCTTTGATCTCGACCAGGAGACTCCAGATAAAGTGCTCCGCAGAATCTATCTTAACCTGGAGAGGCTCAAAGATGATGATTTCGCTCGTCAGATTCGGGAGAAATTAAAAATCATTGTTGCAGGAGGTGATGGCACTGCTGGGTGGCTCCTTGGAGTTGTATGTGACCTTAAATTGTCACATCCTCCTCCAATTGCCACTGTACCTTTGGGTACAGGAAACAACCTTCCCTTTGCTTTTGGATGGGGAAAGAAGAATCCAGGAACAGATAGGACTGCAGTGGAGTCGTTTTTGGAACAAGTGTTGAAGGCAAAAGTGATGAAGATTGACAATTGGCACATACTTATGAGGATGAAAACTCCCAAAGAAGGTGGTTCTTGTGATCCTGTTGCTCCTCTTGAGTTACCACATTCTCTACATGCATTTCACCGTGTTTCTCCAACTGATGAACTAAACAAGGAAGGCTGCCACACTTTTCGAGGAGGGTTCTGGAATTACTTTAGCCTCGGAATGGATGCTCAGATTTCTTATGCGTTTCATTCTGAGAGGAAGCTTCACCCTGAAAAGTTTAAGAATCAGCTGGTTAATCAGAGTACGTATGTAAAGCTTGGTTGCACGCAAGGATGGTTTTGTGCCTCTCTTTTCCACCCTGCTTCACGGAATATAGCTCAGCTTGCCAAGGTTAAGATTGCAACTAGAAATGGCCAGTGGCAGGACCTCCACATACCACATAGCATCAGGTCCATTGTATGTCTGAATCTGCCCAGCTTTTCGGGAGGATTAAATCCTTGGGGCACACCAAATCCCAGGAAACAACGTGATAGAGGCTTGACTCCACCATTTGTAGATGATGGCCTCATTGAGGTTGTTGGGTTTAGAAATGCTTGGCATGGTCTTGTTCTGCTCGCTCCCAATGGACATGGGACACGACTTGCCCAGGCAAATCGTATTCGCTTCGAATTTCACAAAGGTGCAACCGACCATACATTCATGAGGATGGATGGGGAGCCCTGGAAACAGCCACTGCCACTGGATGATGAAACTGTGATGGTAGAGATTTCACACCTTGGCCAAGTGAACATGCTTGCAACTCATGACTGCCGGTCCAGAAGTGTGTTTGACCCTTCAACACCCCGCCATCAGGATGGTGCAGAAGATTATGATGATAATGAAGACGACTCAGTGGCTGAAGGCGAAGAATTTAGAAAGTTTGGTGCTGCGGATACCTTCAAGATTCCTGATGAGGGAGAACACAGTAATAAAAAAGGAAGGGCTTCAAGGAGGAGAAATTCAAATGTTCATGGTTGGTCACATGTGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001775 GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005886 GO:0006629 GO:0006793 GO:0006796 GO:0006950 GO:0007596 GO:0007599 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0030168 GO:0030258 GO:0030425 GO:0031974 GO:0031981 GO:0032501 GO:0032991 GO:0036477 GO:0042060 GO:0042995 GO:0043005 GO:0043197 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044255 GO:0044309 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044456 GO:0044463 GO:0044464 GO:0045202 GO:0046834 GO:0050817 GO:0050878 GO:0050896 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097447 GO:0097458 GO:0098794 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

509

Amino Acids

57.38

Weight (kDa)

6.43

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	40 - 176	3.2e-21	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	232 - 406	5.1e-37	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	118
Acc36I	ACCTGC	1 cut(s)	118
AciI	CCGC	5 cut(s)	68, 147, 247, 1342, 1427
AclWI	GGATC	1 cut(s)	584
AcoI	YGGCCR	2 cut(s)	910, 1276
AcsI	RAATTY	3 cut(s)	1166, 1406, 1495
AcuI	CTGAAG	1 cut(s)	1416
AfaI	GTAC	4 cut(s)	56, 411, 421, 802
AfiI	CCNNNNNNNGG	7 cut(s)	67, 575, 718, 919, 1069, 1348, 1451
AflIII	ACRYGT	1 cut(s)	1519
AgsI	TTSAA	5 cut(s)	514, 1335, 1438, 1485, 1500
AhdI	GACNNNNNGTC	1 cut(s)	1309
AjnI	CCWGG	6 cut(s)	222, 264, 466, 1013, 1143, 1217
AleI	CACNNNNGTG	1 cut(s)	1176
AluBI	AGCT	6 cut(s)	760, 787, 814, 875, 880, 975
AluI	AGCT	6 cut(s)	760, 787, 814, 875, 880, 975
Alw21I	GWGCWC	2 cut(s)	246, 1527
Alw26I	GTCTC	1 cut(s)	221
AlwI	GGATC	1 cut(s)	584
AlwNI	CAGNNNCTG	2 cut(s)	1231, 1394
AoxI	GGCC	3 cut(s)	910, 1060, 1276
ApeKI	GCWGC	2 cut(s)	672, 1424
ApoI	RAATTY	3 cut(s)	1166, 1406, 1495
Asp700I	GAANNNNTTC	2 cut(s)	171, 1480
AspS9I	GGNCC	3 cut(s)	922, 948, 1311
AsuHPI	GGTGA	4 cut(s)	169, 347, 626, 755
AsuII	TTCGAA	1 cut(s)	1164
AvaII	GGWCC	3 cut(s)	922, 948, 1311
BaeGI	GKGCMC	1 cut(s)	1004
BalI	TGGCCA	2 cut(s)	912, 1278
BanII	GRGCYC	2 cut(s)	119, 1218
BbsI	GAAGAC	2 cut(s)	121, 1386
Bbv12I	GWGCWC	2 cut(s)	246, 1527
BbvI	GCAGC	2 cut(s)	659, 1411
BccI	CCATC	9 cut(s)	332, 444, 825, 1052, 1198, 1202, 1249, 1346, 1353
BcgI	CGANNNNNNTGC	2 cut(s)	474, 508
BciT130I	CCWGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
BciVI	GTATCC	1 cut(s)	1423
BcoDI	GTCTC	1 cut(s)	221
BfaI	CTAG	1 cut(s)	903
BfmI	CTRYAG	1 cut(s)	483
BfuAI	ACCTGC	1 cut(s)	118
BfuI	GTATCC	1 cut(s)	1423
BisI	GCNGC	2 cut(s)	673, 1425
BlpI	GCTNAGC	1 cut(s)	876
BlsI	GCNGC	2 cut(s)	674, 1426
Bme1390I	CCNGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
Bme18I	GGWCC	3 cut(s)	922, 948, 1311
BmeRI	GACNNNNNGTC	1 cut(s)	1309
BmgT120I	GGNCC	3 cut(s)	922, 948, 1311
BmiI	GGNNCC	2 cut(s)	356, 1215
BmrFI	CCNGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
BmsI	GCATC	2 cut(s)	712, 951
BpiI	GAAGAC	2 cut(s)	121, 1386
BpmI	CTGGAG	2 cut(s)	216, 287
Bpu1102I	GCTNAGC	1 cut(s)	876
Bpu14I	TTCGAA	1 cut(s)	1164
BpuEI	CTTGAG	2 cut(s)	17, 626
BsaAI	YACGTR	2 cut(s)	54, 804
BsaJI	CCNNGG	8 cut(s)	358, 712, 885, 995, 1013, 1143, 1217, 1273
Bsc4I	CCNNNNNNNGG	7 cut(s)	67, 575, 718, 919, 1069, 1348, 1451
Bse118I	RCCGGY	1 cut(s)	1308
Bse1I	ACTGG	2 cut(s)	913, 1242
BseBI	CCWGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
BseDI	CCNNGG	8 cut(s)	358, 712, 885, 995, 1013, 1143, 1217, 1273
BseLI	CCNNNNNNNGG	7 cut(s)	67, 575, 718, 919, 1069, 1348, 1451
BseMII	CTCAG	4 cut(s)	740, 741, 890, 1401
BseNI	ACTGG	2 cut(s)	913, 1242
BseRI	GAGGAG	9 cut(s)	95, 98, 101, 104, 107, 384, 591, 702, 1504
BseSI	GKGCMC	1 cut(s)	1004
BseXI	GCAGC	2 cut(s)	659, 1411
BseYI	CCCAGC	2 cut(s)	347, 971
BsgI	GTGCAG	1 cut(s)	1377
BshFI	GGCC	3 cut(s)	912, 1062, 1278
BsiHKAI	GWGCWC	2 cut(s)	246, 1527
BsiSI	CCGG	1 cut(s)	1309
BsiWI	CGTACG	1 cut(s)	54
BslFI	GGGAC	1 cut(s)	1144
BslI	CCNNNNNNNGG	7 cut(s)	67, 575, 718, 919, 1069, 1348, 1451
BsmAI	GTCTC	1 cut(s)	221
BsmFI	GGGAC	1 cut(s)	1144
BsnI	GGCC	3 cut(s)	912, 1062, 1278
Bsp119I	TTCGAA	1 cut(s)	1164
Bsp1286I	GDGCHC	5 cut(s)	119, 246, 1004, 1218, 1527
Bsp143I	GATC	3 cut(s)	185, 214, 589
Bsp1720I	GCTNAGC	1 cut(s)	876
BspACI	CCGC	5 cut(s)	68, 147, 247, 1342, 1427
BspANI	GGCC	3 cut(s)	912, 1062, 1278
BspCNI	CTCAG	4 cut(s)	739, 742, 889, 1400
BspHI	TCATGA	2 cut(s)	1197, 1300
BspLI	GGNNCC	2 cut(s)	356, 1215
BspMAI	CTGCAG	1 cut(s)	487
BspMI	ACCTGC	1 cut(s)	118
BspPI	GGATC	1 cut(s)	584
BspT104I	TTCGAA	1 cut(s)	1164
BsrFI	RCCGGY	1 cut(s)	1308
BsrI	ACTGG	2 cut(s)	913, 1242
BssAI	RCCGGY	1 cut(s)	1308
BssECI	CCNNGG	8 cut(s)	358, 712, 885, 995, 1013, 1143, 1217, 1273
BssMI	GATC	3 cut(s)	185, 214, 589
BssT1I	CCWWGG	4 cut(s)	358, 885, 995, 1273
Bst2UI	CCWGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
Bst4CI	ACNGT	5 cut(s)	17, 409, 638, 1252, 1463
Bst6I	CTCTTC	2 cut(s)	72, 195
BstBAI	YACGTR	2 cut(s)	54, 804
BstBI	TTCGAA	1 cut(s)	1164
BstC8I	GCNNGC	5 cut(s)	115, 824, 882, 1113, 1293
BstDEI	CTNAG	4 cut(s)	726, 750, 876, 1387
BstENI	CCTNNNNNAGG	1 cut(s)	1067
BstKTI	GATC	3 cut(s)	188, 217, 592
BstMAI	GTCTC	1 cut(s)	221
BstMBI	GATC	3 cut(s)	185, 214, 589
BstMWI	GCNNNNNNNGC	1 cut(s)	881
BstNI	CCWGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
BstNSI	RCATGY	3 cut(s)	629, 1291, 1523
BstSCI	CCNGG	6 cut(s)	222, 264, 466, 1013, 1143, 1217
BstSFI	CTRYAG	1 cut(s)	483
BstSLI	GKGCMC	1 cut(s)	1004
BstSNI	TACGTA	2 cut(s)	54, 804
BstV1I	GCAGC	2 cut(s)	659, 1411
BstV2I	GAAGAC	2 cut(s)	121, 1386
BstXI	CCANNNNNNTGG	1 cut(s)	1352
BsuI	GTATCC	1 cut(s)	1423
BsuRI	GGCC	3 cut(s)	912, 1062, 1278
BtsI	GCAGTG	3 cut(s)	342, 492, 1229
BtsIMutI	CAGTG	7 cut(s)	342, 405, 492, 920, 1229, 1235, 1395
BveI	ACCTGC	1 cut(s)	118
Cac8I	GCNNGC	5 cut(s)	115, 824, 882, 1113, 1293
CaiI	CAGNNNCTG	2 cut(s)	1231, 1394
CciI	TCATGA	2 cut(s)	1197, 1300
Cfr10I	RCCGGY	1 cut(s)	1308
Cfr13I	GGNCC	3 cut(s)	922, 948, 1311
Csp6I	GTAC	4 cut(s)	55, 410, 420, 801
CspCI	CAANNNNNGTGG	4 cut(s)	130, 165, 559, 594
CviAII	CATG	8 cut(s)	626, 1097, 1127, 1198, 1288, 1301, 1508, 1520
CviQI	GTAC	4 cut(s)	55, 410, 420, 801
DdeI	CTNAG	4 cut(s)	726, 750, 876, 1387
DpnI	GATC	3 cut(s)	187, 216, 591
DpnII	GATC	3 cut(s)	185, 214, 589
DriI	GACNNNNNGTC	1 cut(s)	1309
EaeI	YGGCCR	2 cut(s)	910, 1276
Eam1104I	CTCTTC	2 cut(s)	72, 195
Eam1105I	GACNNNNNGTC	1 cut(s)	1309
EarI	CTCTTC	2 cut(s)	72, 195
EciI	GGCGGA	1 cut(s)	83
Eco105I	TACGTA	2 cut(s)	54, 804
Eco130I	CCWWGG	4 cut(s)	358, 885, 995, 1273
Eco24I	GRGCYC	2 cut(s)	119, 1218
Eco47I	GGWCC	3 cut(s)	922, 948, 1311
Eco57I	CTGAAG	1 cut(s)	1416
EcoNI	CCTNNNNNAGG	1 cut(s)	1067
EcoO109I	RGGNCCY	1 cut(s)	922
EcoRII	CCWGG	6 cut(s)	222, 264, 466, 1013, 1143, 1217
EcoT14I	CCWWGG	4 cut(s)	358, 885, 995, 1273
EcoT22I	ATGCAT	1 cut(s)	631
EcoT38I	GRGCYC	2 cut(s)	119, 1218
ErhI	CCWWGG	4 cut(s)	358, 885, 995, 1273
FaeI	CATG	8 cut(s)	629, 1100, 1130, 1201, 1291, 1304, 1511, 1523
FaqI	GGGAC	1 cut(s)	1144
FatI	CATG	8 cut(s)	625, 1096, 1126, 1197, 1287, 1300, 1507, 1519
FauI	CCCGC	1 cut(s)	1349
Fnu4HI	GCNGC	2 cut(s)	673, 1425
FriOI	GRGCYC	2 cut(s)	119, 1218
Fsp4HI	GCNGC	2 cut(s)	673, 1425
FspBI	CTAG	1 cut(s)	903
GluI	GCNGC	2 cut(s)	673, 1425
GsaI	CCCAGC	2 cut(s)	351, 975
GsuI	CTGGAG	2 cut(s)	216, 287
HaeIII	GGCC	3 cut(s)	912, 1062, 1278
HapII	CCGG	1 cut(s)	1309
Hin1II	CATG	8 cut(s)	629, 1100, 1130, 1201, 1291, 1304, 1511, 1523
HindIII	AAGCTT	2 cut(s)	758, 812
HpaII	CCGG	1 cut(s)	1309
HphI	GGTGA	4 cut(s)	169, 347, 626, 755
Hpy166II	GTNNAC	1 cut(s)	1285
Hpy188I	TCNGA	8 cut(s)	25, 300, 716, 729, 751, 798, 963, 1529
Hpy8I	GTNNAC	1 cut(s)	1285
HpyAV	CCTTC	8 cut(s)	443, 508, 569, 662, 1341, 1391, 1444, 1470
HpyCH4III	ACNGT	5 cut(s)	17, 409, 638, 1252, 1463
HpyCH4IV	ACGT	4 cut(s)	53, 57, 803, 1024
HpyCH4V	TGCA	8 cut(s)	329, 485, 629, 822, 899, 1181, 1295, 1358
HpyF10VI	GCNNNNNNNGC	1 cut(s)	881
HpyF3I	CTNAG	4 cut(s)	726, 750, 876, 1387
HpySE526I	ACGT	4 cut(s)	53, 57, 803, 1024
Hsp92II	CATG	8 cut(s)	629, 1100, 1130, 1201, 1291, 1304, 1511, 1523
Kzo9I	GATC	3 cut(s)	185, 214, 589
LmnI	GCTCC	5 cut(s)	249, 360, 604, 1120, 1213
Lsp1109I	GCAGC	2 cut(s)	659, 1411
LweI	GCATC	2 cut(s)	712, 951
MaeI	CTAG	1 cut(s)	903
MaeII	ACGT	4 cut(s)	53, 57, 803, 1024
MaeIII	GTNAC	4 cut(s)	371, 384, 609, 1515
MalI	GATC	3 cut(s)	187, 216, 591
MboI	GATC	3 cut(s)	185, 214, 589
MboII	GAAGA	8 cut(s)	89, 121, 182, 472, 541, 1373, 1391, 1415
MfeI	CAATTG	2 cut(s)	399, 538
MhlI	GDGCHC	5 cut(s)	119, 246, 1004, 1218, 1527
MlsI	TGGCCA	2 cut(s)	912, 1278
MluCI	AATT	8 cut(s)	311, 380, 399, 538, 700, 1166, 1406, 1495
MluNI	TGGCCA	2 cut(s)	912, 1278
MlyI	GAGTC	5 cut(s)	106, 223, 500, 1033, 1379
MmeI	TCCRAC	1 cut(s)	671
Mox20I	TGGCCA	2 cut(s)	912, 1278
Mph1103I	ATGCAT	1 cut(s)	631
MroXI	GAANNNNTTC	2 cut(s)	171, 1480
MscI	TGGCCA	2 cut(s)	912, 1278
MseI	TTAA	7 cut(s)	261, 314, 378, 777, 792, 891, 989
MslI	CAYNNNNRTG	4 cut(s)	551, 1176, 1202, 1350
Msp20I	TGGCCA	2 cut(s)	912, 1278
MspA1I	CMGCKG	1 cut(s)	787
MspI	CCGG	1 cut(s)	1309
MspR9I	CCNGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
MunI	CAATTG	2 cut(s)	399, 538
MvaI	CCWGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
MwoI	GCNNNNNNNGC	1 cut(s)	881
NdeII	GATC	3 cut(s)	185, 214, 589
NlaIII	CATG	8 cut(s)	629, 1100, 1130, 1201, 1291, 1304, 1511, 1523
NlaIV	GGNNCC	2 cut(s)	356, 1215
NmeAIII	GCCGAG	1 cut(s)	44
NmuCI	GTSAC	3 cut(s)	371, 384, 1515
NsiI	ATGCAT	1 cut(s)	631
NspI	RCATGY	3 cut(s)	629, 1291, 1523
NspV	TTCGAA	1 cut(s)	1164
OliI	CACNNNNGTG	1 cut(s)	1176
PagI	TCATGA	2 cut(s)	1197, 1300
PaqCI	CACCTGC	1 cut(s)	118
PciI	ACATGT	1 cut(s)	1519
PdmI	GAANNNNTTC	2 cut(s)	171, 1480
PfeI	GAWTC	6 cut(s)	252, 301, 463, 781, 964, 1441
Pfl23II	CGTACG	1 cut(s)	54
PfoI	TCCNGGA	1 cut(s)	466
PkrI	GCNGC	2 cut(s)	674, 1426
PleI	GAGTC	5 cut(s)	105, 223, 499, 1033, 1379
PpsI	GAGTC	5 cut(s)	105, 223, 499, 1033, 1379
Ppu21I	YACGTR	2 cut(s)	54, 804
PpuMI	RGGWCCY	1 cut(s)	922
PscI	ACATGT	1 cut(s)	1519
Psp5II	RGGWCCY	1 cut(s)	922
Psp6I	CCWGG	6 cut(s)	222, 264, 466, 1013, 1143, 1217
PspFI	CCCAGC	2 cut(s)	347, 971
PspGI	CCWGG	6 cut(s)	222, 264, 466, 1013, 1143, 1217
PspLI	CGTACG	1 cut(s)	54
PspN4I	GGNNCC	2 cut(s)	356, 1215
PspPI	GGNCC	3 cut(s)	922, 948, 1311
PspPPI	RGGWCCY	1 cut(s)	922
PstI	CTGCAG	1 cut(s)	487
PstNI	CAGNNNCTG	2 cut(s)	1231, 1394
PvuII	CAGCTG	1 cut(s)	787
RsaI	GTAC	4 cut(s)	56, 411, 421, 802
RsaNI	GTAC	4 cut(s)	55, 410, 420, 801
RseI	CAYNNNNRTG	4 cut(s)	551, 1176, 1202, 1350
SaqAI	TTAA	7 cut(s)	261, 314, 378, 777, 792, 891, 989
SatI	GCNGC	2 cut(s)	673, 1425
Sau3AI	GATC	3 cut(s)	185, 214, 589
Sau96I	GGNCC	3 cut(s)	922, 948, 1311
SchI	GAGTC	5 cut(s)	106, 223, 500, 1033, 1379
ScrFI	CCNGG	6 cut(s)	224, 266, 468, 1015, 1145, 1219
SduI	GDGCHC	5 cut(s)	119, 246, 1004, 1218, 1527
SfaNI	GCATC	2 cut(s)	712, 951
SfcI	CTRYAG	1 cut(s)	483
SfuI	TTCGAA	1 cut(s)	1164
SinI	GGWCC	3 cut(s)	922, 948, 1311
SmiMI	CAYNNNNRTG	4 cut(s)	551, 1176, 1202, 1350
SmlI	CTYRAG	2 cut(s)	32, 605
SmoI	CTYRAG	2 cut(s)	32, 605
SnaBI	TACGTA	2 cut(s)	54, 804
Sse9I	AATT	8 cut(s)	311, 380, 399, 538, 700, 1166, 1406, 1495
SsiI	CCGC	5 cut(s)	68, 147, 247, 1342, 1427
SspMI	CTAG	1 cut(s)	903
StyD4I	CCNGG	6 cut(s)	222, 264, 466, 1013, 1143, 1217
StyI	CCWWGG	4 cut(s)	358, 885, 995, 1273
TaaI	ACNGT	5 cut(s)	17, 409, 638, 1252, 1463
TaiI	ACGT	4 cut(s)	56, 60, 806, 1027
TaqI	TCGA	3 cut(s)	219, 685, 1164
TasI	AATT	8 cut(s)	311, 380, 399, 538, 700, 1166, 1406, 1495
TfiI	GAWTC	6 cut(s)	252, 301, 463, 781, 964, 1441
Tru1I	TTAA	7 cut(s)	261, 314, 378, 777, 792, 891, 989
Tru9I	TTAA	7 cut(s)	261, 314, 378, 777, 792, 891, 989
TscAI	CASTG	7 cut(s)	349, 412, 492, 920, 1236, 1242, 1395
TseFI	GTSAC	3 cut(s)	371, 384, 1515
TseI	GCWGC	2 cut(s)	672, 1424
Tsp45I	GTSAC	3 cut(s)	371, 384, 1515
TspDTI	ATGAA	9 cut(s)	121, 542, 575, 669, 734, 1186, 1260, 1392, 1496
TspGWI	ACGGA	1 cut(s)	880
TspRI	CASTG	7 cut(s)	349, 412, 492, 920, 1236, 1242, 1395
VpaK11BI	GGWCC	3 cut(s)	922, 948, 1311
XagI	CCTNNNNNAGG	1 cut(s)	1067
XapI	RAATTY	3 cut(s)	1166, 1406, 1495
XceI	RCATGY	3 cut(s)	629, 1291, 1523
XcmI	CCANNNNNNNNNTGG	1 cut(s)	412
XmnI	GAANNNNTTC	2 cut(s)	171, 1480
XspI	CTAG	1 cut(s)	903
Zsp2I	ATGCAT	1 cut(s)	631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.