Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

30241688 .. 30246634

Gene Structure 4947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG256700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGAGTTCCGGAATCGAATTTCCGGTTGCCGCTTGCGAGACTCTATACACGGTCTCGTTTCGAGGAGTTGGGAGGCAATGGTGGGAGATAGAGGCAAGTTGAAGGATTTTTACATTCCGAATTACATACTTGTGGGCGGGGCGGTGCCGAAGATCAAATTTGCACCTGCGTGTCCGGTCATAGTGTTTGTTAATACTAGAAGTGGTGGTCAGCTTGGAGGTGAACTTGTTCTTACATATAGAGCCCTTCTCAATGAGCACCAGGTATTTGACTTGGGACAAACGGCTCCTGACAAGGTGCTTCACAAGCTCTATGTTGCTTTGGAAACACTCAAGGGCGGCGGAGATGTTTTGGCTGCTGATATCCAGAAGAGGTTAAGAATAATTGCTGCAGGTGGAGATGGTACAGCTGGCTGGCTCCTCAGTGTAGTTTCTGATCTTAAACTGCCTAACCCACCTCCAATCGCCACGATGCCATTGGGAACTGGAAATAATGTCCACTTCGCGTTTGGATGGGGAAAGAAAAATCCTGGGACAGACCGACAATCTGTGCAGTATTTCTTGAACCAAGTGAAGACAGCAAAGGAAATGCAAATTGACAGCTGGCACATCATTATGAGAATGAGGACTCCAAAGGAAGGTTCATGTGATCCTATTGCACCTCTGGACCTACCGCATTCTTTGCATGCATTTCATCAGGAGGGCTTCCAGACATTTCGTGGGGGATTTTGGAACTACTTTAGCATGGGAATGGATGCTCAAATATCATATGATTTTCATGCTCAGAGAAAGTTGCACGCTGAAAAGTTTAACAACCAGTTAATTAATCAAAGTACTTACTTCAAGCTTGGATGTACTCAAGGATGGTTCTTTGCTTCTCTAATGCATCCTTCTTCAAGGAATATAGCACATCTGACAAAGATTAAGATCATGAAAAGACAAGGTCATTGGGAGGAGCTTCACATATCTCCCAGCATTAGGTCTATTGTTTGCCTCAATTTACCCAGTTTTTCTGGTGGTCTTGATCCATGGGGGAAGCCAAATAGAAAGAAACTGCGATATCGGGATTTAACTCCTCCATATGTGGATGATGGCCTTCTTGAGATTGTTGGCTTTAGAGATGCCTGGCATGGGCTTGCGTTACTTGCTCCTAAGGGACATGGGACTCGTCTTGCACAAGCACACGGAATACGTTTTGAGTTTTGCAAGGGCGCAGTCGACCATACATTCATGAGAATTGATGGGGAGCCATGGAAACAACCTCTTCCAGTTGACGATGACACGGTGGTTGTAGAAATTTCTCACTTTGGCCAAGTAAGCATGCTTGCCACGCCATCGAAGCGATCCAAAAGTATATCTGACCCTGCGTCACCAGCTAGCTCCCATGATGACGATGATGATAGTAGTAATGAGGAAGAATATCCAGAAGATAGTGGAGAGAGAAGGAAGTTTGGTGCATCTGACACATTTAAAATCCCGGACGGGATTGACATTGCTCATTTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007610 GO:0007635 GO:0008150 GO:0009266 GO:0009605 GO:0009628 GO:0010646 GO:0023051 GO:0040011 GO:0042048 GO:0042221 GO:0042330 GO:0043052 GO:0048167 GO:0048168 GO:0048169 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0065007 GO:0065008 GO:0099177

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

502

Amino Acids

56.01

Weight (kDa)

8.38

Isoelectric Point (pI)

37.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	59 - 179	5.7e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	243 - 417	4.5e-36	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	175, 383
Acc36I	ACCTGC	2 cut(s)	175, 383
AccB1I	GGYRCC	1 cut(s)	145
AccI	GTMKAC	1 cut(s)	1218
AccII	CGCG	1 cut(s)	506
AccIII	TCCGGA	1 cut(s)	9
AciI	CCGC	6 cut(s)	31, 138, 143, 339, 342, 674
AclWI	GGATC	3 cut(s)	644, 1019, 1338
AcoI	YGGCCR	1 cut(s)	1309
AcsI	RAATTY	3 cut(s)	18, 158, 1296
AfaI	GTAC	3 cut(s)	406, 835, 856
AfiI	CCNNNNNNNGG	3 cut(s)	638, 978, 1131
AgsI	TTSAA	4 cut(s)	103, 565, 844, 897
AhdI	GACNNNNNGTC	1 cut(s)	1366
AjnI	CCWGG	3 cut(s)	261, 529, 1124
AjuI	GAANNNNNNNTTGG	2 cut(s)	625, 657
AleI	CACNNNNGTG	1 cut(s)	169
AluBI	AGCT	8 cut(s)	214, 310, 410, 603, 847, 958, 1376, 1380
AluI	AGCT	8 cut(s)	214, 310, 410, 603, 847, 958, 1376, 1380
Alw21I	GWGCWC	1 cut(s)	261
Alw26I	GTCTC	2 cut(s)	33, 59
AlwI	GGATC	3 cut(s)	644, 1019, 1338
Aor13HI	TCCGGA	1 cut(s)	9
AoxI	GGCC	2 cut(s)	1093, 1309
ApeKI	GCWGC	2 cut(s)	356, 389
ApoI	RAATTY	3 cut(s)	18, 158, 1296
AseI	ATTAAT	1 cut(s)	825
Asp700I	GAANNNNTTC	1 cut(s)	228
AspLEI	GCGC	1 cut(s)	1214
AspS9I	GGNCC	1 cut(s)	667
AsuC2I	CCSGG	1 cut(s)	1478
AsuHPI	GGTGA	2 cut(s)	233, 1362
AsuNHI	GCTAGC	1 cut(s)	1376
AvaII	GGWCC	1 cut(s)	667
AxyI	CCTNAGG	1 cut(s)	1152
BalI	TGGCCA	1 cut(s)	1311
BanI	GGYRCC	1 cut(s)	145
BanII	GRGCYC	1 cut(s)	247
BarI	GAAGNNNNNNTAC	2 cut(s)	95, 127
BbsI	GAAGAC	1 cut(s)	581
Bbv12I	GWGCWC	1 cut(s)	261
BbvI	GCAGC	2 cut(s)	343, 376
BccI	CCATC	6 cut(s)	395, 507, 858, 1085, 1235, 1342
BceAI	ACGGC	1 cut(s)	300
BciT130I	CCWGG	3 cut(s)	263, 531, 1126
BcnI	CCSGG	1 cut(s)	1478
BcoDI	GTCTC	2 cut(s)	33, 59
BfaI	CTAG	2 cut(s)	198, 1377
BfmI	CTRYAG	1 cut(s)	390
BfuAI	ACCTGC	2 cut(s)	175, 383
BisI	GCNGC	4 cut(s)	31, 340, 357, 390
BlsI	GCNGC	4 cut(s)	32, 341, 358, 391
BmcAI	AGTACT	1 cut(s)	835
Bme1390I	CCNGG	4 cut(s)	263, 531, 1126, 1478
Bme18I	GGWCC	1 cut(s)	667
BmeRI	GACNNNNNGTC	1 cut(s)	1366
BmgT120I	GGNCC	1 cut(s)	667
BmiI	GGNNCC	4 cut(s)	147, 288, 419, 1248
BmrFI	CCNGG	4 cut(s)	263, 531, 1126, 1478
BmrI	ACTGGG	1 cut(s)	999
BmsI	GCATC	5 cut(s)	462, 745, 895, 1111, 1466
BmtI	GCTAGC	1 cut(s)	1380
BmuI	ACTGGG	1 cut(s)	999
BpiI	GAAGAC	1 cut(s)	581
BplI	GAGNNNNNCTC	2 cut(s)	234, 266
BpuEI	CTTGAG	3 cut(s)	317, 843, 1121
BpuMI	CCSGG	1 cut(s)	1478
BsaBI	GATNNNNATC	1 cut(s)	926
BsaI	GGTCTC	1 cut(s)	59
BsaJI	CCNNGG	3 cut(s)	530, 1028, 1250
BsaWI	WCCGGW	3 cut(s)	9, 23, 175
Bsc4I	CCNNNNNNNGG	3 cut(s)	638, 978, 1131
Bse1I	ACTGG	4 cut(s)	490, 817, 1005, 1268
Bse21I	CCTNAGG	1 cut(s)	1152
Bse3DI	GCAATG	2 cut(s)	84, 1491
Bse8I	GATNNNNATC	1 cut(s)	926
BseAI	TCCGGA	1 cut(s)	9
BseBI	CCWGG	3 cut(s)	263, 531, 1126
BseDI	CCNNGG	3 cut(s)	530, 1028, 1250
BseGI	GGATG	6 cut(s)	518, 760, 857, 869, 886, 1093
BseJI	GATNNNNATC	1 cut(s)	926
BseLI	CCNNNNNNNGG	3 cut(s)	638, 978, 1131
BseMI	GCAATG	2 cut(s)	84, 1491
BseMII	CTCAG	2 cut(s)	436, 797
BseNI	ACTGG	4 cut(s)	490, 817, 1005, 1268
BseRI	GAGGAG	4 cut(s)	79, 410, 968, 1065
BseXI	GCAGC	2 cut(s)	343, 376
BseYI	CCCAGC	1 cut(s)	971
BsgI	GTGCAG	1 cut(s)	572
Bsh1236I	CGCG	1 cut(s)	506
BshFI	GGCC	2 cut(s)	1095, 1311
BshNI	GGYRCC	1 cut(s)	145
BsiHKAI	GWGCWC	1 cut(s)	261
BsiSI	CCGG	4 cut(s)	10, 24, 176, 1478
BslFI	GGGAC	4 cut(s)	291, 547, 1170, 1177
BslI	CCNNNNNNNGG	3 cut(s)	638, 978, 1131
BsmAI	GTCTC	2 cut(s)	33, 59
BsmFI	GGGAC	4 cut(s)	291, 547, 1170, 1177
BsmI	GAATGC	1 cut(s)	676
BsnI	GGCC	2 cut(s)	1095, 1311
Bso31I	GGTCTC	1 cut(s)	59
Bsp1286I	GDGCHC	2 cut(s)	247, 261
Bsp13I	TCCGGA	1 cut(s)	9
Bsp143I	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
Bsp19I	CCATGG	2 cut(s)	1028, 1250
BspACI	CCGC	6 cut(s)	31, 138, 143, 339, 342, 674
BspANI	GGCC	2 cut(s)	1095, 1311
BspCNI	CTCAG	2 cut(s)	435, 796
BspEI	TCCGGA	1 cut(s)	9
BspFNI	CGCG	1 cut(s)	506
BspHI	TCATGA	2 cut(s)	930, 1230
BspLI	GGNNCC	4 cut(s)	147, 288, 419, 1248
BspMAI	CTGCAG	1 cut(s)	394
BspMI	ACCTGC	2 cut(s)	175, 383
BspOI	GCTAGC	1 cut(s)	1380
BspPI	GGATC	3 cut(s)	644, 1019, 1338
BspT107I	GGYRCC	1 cut(s)	145
BspTNI	GGTCTC	1 cut(s)	59
BsrDI	GCAATG	2 cut(s)	84, 1491
BsrI	ACTGG	4 cut(s)	490, 817, 1005, 1268
BssECI	CCNNGG	3 cut(s)	530, 1028, 1250
BssMI	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
BssT1I	CCWWGG	2 cut(s)	1028, 1250
Bst2UI	CCWGG	3 cut(s)	263, 531, 1126
Bst4CI	ACNGT	2 cut(s)	53, 1285
Bst6I	CTCTTC	2 cut(s)	365, 1269
BstAPI	GCANNNNNTGC	1 cut(s)	682
BstDEI	CTNAG	3 cut(s)	422, 783, 1152
BstDSI	CCRYGG	2 cut(s)	1028, 1250
BstF5I	GGATG	6 cut(s)	518, 760, 857, 869, 886, 1093
BstFNI	CGCG	1 cut(s)	506
BstHHI	GCGC	1 cut(s)	1214
BstKTI	GATC	6 cut(s)	156, 439, 652, 930, 1027, 1346
BstMAI	GTCTC	2 cut(s)	33, 59
BstMBI	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
BstMWI	GCNNNNNNNGC	7 cut(s)	307, 682, 1145, 1317, 1330, 1339, 1373
BstNI	CCWGG	3 cut(s)	263, 531, 1126
BstNSI	RCATGY	2 cut(s)	689, 1324
BstSCI	CCNGG	4 cut(s)	261, 529, 1124, 1476
BstSFI	CTRYAG	1 cut(s)	390
BstUI	CGCG	1 cut(s)	506
BstV1I	GCAGC	2 cut(s)	343, 376
BstV2I	GAAGAC	1 cut(s)	581
Bsu36I	CCTNAGG	1 cut(s)	1152
BsuRI	GGCC	2 cut(s)	1095, 1311
BtgI	CCRYGG	2 cut(s)	1028, 1250
BtsCI	GGATG	6 cut(s)	518, 760, 857, 869, 886, 1093
BtsIMutI	CAGTG	1 cut(s)	430
BveI	ACCTGC	2 cut(s)	175, 383
CciI	TCATGA	2 cut(s)	930, 1230
CfoI	GCGC	1 cut(s)	1214
Cfr13I	GGNCC	1 cut(s)	667
CseI	GACGC	1 cut(s)	1356
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	3 cut(s)	405, 834, 855
CviQI	GTAC	3 cut(s)	405, 834, 855
DdeI	CTNAG	3 cut(s)	422, 783, 1152
DpnI	GATC	6 cut(s)	155, 438, 651, 929, 1026, 1345
DpnII	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
DraI	TTTAAA	1 cut(s)	1471
DriI	GACNNNNNGTC	1 cut(s)	1366
EaeI	YGGCCR	1 cut(s)	1309
Eam1104I	CTCTTC	2 cut(s)	365, 1269
Eam1105I	GACNNNNNGTC	1 cut(s)	1366
EarI	CTCTTC	2 cut(s)	365, 1269
EciI	GGCGGA	1 cut(s)	357
Eco130I	CCWWGG	2 cut(s)	1028, 1250
Eco24I	GRGCYC	1 cut(s)	247
Eco31I	GGTCTC	1 cut(s)	59
Eco32I	GATATC	2 cut(s)	364, 1061
Eco47I	GGWCC	1 cut(s)	667
Eco81I	CCTNAGG	1 cut(s)	1152
EcoRII	CCWGG	3 cut(s)	261, 529, 1124
EcoRV	GATATC	2 cut(s)	364, 1061
EcoT14I	CCWWGG	2 cut(s)	1028, 1250
EcoT22I	ATGCAT	2 cut(s)	691, 888
EcoT38I	GRGCYC	1 cut(s)	247
ErhI	CCWWGG	2 cut(s)	1028, 1250
FaqI	GGGAC	4 cut(s)	291, 547, 1170, 1177
FauI	CCCGC	1 cut(s)	131
FauNDI	CATATG	2 cut(s)	769, 1081
FblI	GTMKAC	1 cut(s)	1218
Fnu4HI	GCNGC	4 cut(s)	31, 340, 357, 390
FokI	GGATG	6 cut(s)	525, 767, 864, 873, 876, 1100
FriOI	GRGCYC	1 cut(s)	247
Fsp4HI	GCNGC	4 cut(s)	31, 340, 357, 390
FspBI	CTAG	2 cut(s)	198, 1377
GlaI	GCGC	1 cut(s)	1213
GluI	GCNGC	4 cut(s)	31, 340, 357, 390
GsaI	CCCAGC	1 cut(s)	975
HaeIII	GGCC	2 cut(s)	1095, 1311
HapII	CCGG	4 cut(s)	10, 24, 176, 1478
HgaI	GACGC	1 cut(s)	1356
HhaI	GCGC	1 cut(s)	1214
Hin6I	GCGC	1 cut(s)	1212
HinP1I	GCGC	1 cut(s)	1212
HincII	GTYRAC	2 cut(s)	1219, 1273
HindII	GTYRAC	2 cut(s)	1219, 1273
HindIII	AAGCTT	1 cut(s)	845
HinfI	GANTC	4 cut(s)	13, 41, 628, 1165
HpaII	CCGG	4 cut(s)	10, 24, 176, 1478
HphI	GGTGA	2 cut(s)	233, 1362
Hpy166II	GTNNAC	4 cut(s)	224, 499, 1219, 1273
Hpy188I	TCNGA	6 cut(s)	120, 436, 786, 915, 1360, 1462
Hpy8I	GTNNAC	4 cut(s)	224, 499, 1219, 1273
HpyAV	CCTTC	6 cut(s)	97, 257, 632, 900, 1106, 1437
HpyCH4III	ACNGT	2 cut(s)	53, 1285
HpyCH4IV	ACGT	1 cut(s)	1192
HpyF10VI	GCNNNNNNNGC	7 cut(s)	307, 682, 1145, 1317, 1330, 1339, 1373
HpyF3I	CTNAG	3 cut(s)	422, 783, 1152
HpySE526I	ACGT	1 cut(s)	1192
HspAI	GCGC	1 cut(s)	1212
Kpn2I	TCCGGA	1 cut(s)	9
Kzo9I	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
LmnI	GCTCC	6 cut(s)	292, 423, 955, 1153, 1246, 1385
Lsp1109I	GCAGC	2 cut(s)	343, 376
LweI	GCATC	5 cut(s)	462, 745, 895, 1111, 1466
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	2 cut(s)	198, 1377
MaeII	ACGT	1 cut(s)	1192
MaeIII	GTNAC	2 cut(s)	1140, 1368
MalI	GATC	6 cut(s)	155, 438, 651, 929, 1026, 1345
MboI	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
MboII	GAAGA	7 cut(s)	163, 382, 586, 885, 1256, 1427, 1439
MhlI	GDGCHC	2 cut(s)	247, 261
MlsI	TGGCCA	1 cut(s)	1311
MluCI	AATT	9 cut(s)	18, 121, 158, 384, 594, 822, 997, 1236, 1296
MluNI	TGGCCA	1 cut(s)	1311
MlyI	GAGTC	3 cut(s)	35, 622, 1159
Mox20I	TGGCCA	1 cut(s)	1311
Mph1103I	ATGCAT	2 cut(s)	691, 888
MroI	TCCGGA	1 cut(s)	9
MroXI	GAANNNNTTC	1 cut(s)	228
MscI	TGGCCA	1 cut(s)	1311
MseI	TTAA	9 cut(s)	192, 377, 441, 810, 821, 825, 924, 1070, 1470
MslI	CAYNNNNRTG	4 cut(s)	131, 169, 614, 749
Msp20I	TGGCCA	1 cut(s)	1311
MspA1I	CMGCKG	2 cut(s)	410, 603
MspI	CCGG	4 cut(s)	10, 24, 176, 1478
MspR9I	CCNGG	4 cut(s)	263, 531, 1126, 1478
Mva1269I	GAATGC	1 cut(s)	676
MvaI	CCWGG	3 cut(s)	263, 531, 1126
MvnI	CGCG	1 cut(s)	506
MwoI	GCNNNNNNNGC	7 cut(s)	307, 682, 1145, 1317, 1330, 1339, 1373
NciI	CCSGG	1 cut(s)	1478
NcoI	CCATGG	2 cut(s)	1028, 1250
NdeI	CATATG	2 cut(s)	769, 1081
NdeII	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
NheI	GCTAGC	1 cut(s)	1376
NlaIV	GGNNCC	4 cut(s)	147, 288, 419, 1248
NmuCI	GTSAC	1 cut(s)	1368
NsiI	ATGCAT	2 cut(s)	691, 888
NspI	RCATGY	2 cut(s)	689, 1324
OliI	CACNNNNGTG	1 cut(s)	169
PacI	TTAATTAA	1 cut(s)	825
PaeI	GCATGC	2 cut(s)	689, 1324
PagI	TCATGA	2 cut(s)	930, 1230
PaqCI	CACCTGC	2 cut(s)	175, 383
PctI	GAATGC	1 cut(s)	676
PdmI	GAANNNNTTC	1 cut(s)	228
PfeI	GAWTC	1 cut(s)	13
PflFI	GACNNNGTC	1 cut(s)	942
PfoI	TCCNGGA	1 cut(s)	1476
PkrI	GCNGC	4 cut(s)	32, 341, 358, 391
PleI	GAGTC	3 cut(s)	35, 622, 1159
PpsI	GAGTC	3 cut(s)	35, 622, 1159
PshBI	ATTAAT	1 cut(s)	825
Psp6I	CCWGG	3 cut(s)	261, 529, 1124
PspFI	CCCAGC	1 cut(s)	971
PspGI	CCWGG	3 cut(s)	261, 529, 1124
PspN4I	GGNNCC	4 cut(s)	147, 288, 419, 1248
PspPI	GGNCC	1 cut(s)	667
PstI	CTGCAG	1 cut(s)	394
PsyI	GACNNNGTC	1 cut(s)	942
PvuII	CAGCTG	2 cut(s)	410, 603
RsaI	GTAC	3 cut(s)	406, 835, 856
RsaNI	GTAC	3 cut(s)	405, 834, 855
RseI	CAYNNNNRTG	4 cut(s)	131, 169, 614, 749
SalI	GTCGAC	1 cut(s)	1217
SaqAI	TTAA	9 cut(s)	192, 377, 441, 810, 821, 825, 924, 1070, 1470
SatI	GCNGC	4 cut(s)	31, 340, 357, 390
Sau3AI	GATC	6 cut(s)	153, 436, 649, 927, 1024, 1343
Sau96I	GGNCC	1 cut(s)	667
ScaI	AGTACT	1 cut(s)	835
SchI	GAGTC	3 cut(s)	35, 622, 1159
ScrFI	CCNGG	4 cut(s)	263, 531, 1126, 1478
SduI	GDGCHC	2 cut(s)	247, 261
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	5 cut(s)	462, 745, 895, 1111, 1466
SfcI	CTRYAG	1 cut(s)	390
SinI	GGWCC	1 cut(s)	667
SmiMI	CAYNNNNRTG	4 cut(s)	131, 169, 614, 749
SmlI	CTYRAG	3 cut(s)	332, 858, 1100
SmoI	CTYRAG	3 cut(s)	332, 858, 1100
SphI	GCATGC	2 cut(s)	689, 1324
Sse9I	AATT	9 cut(s)	18, 121, 158, 384, 594, 822, 997, 1236, 1296
SsiI	CCGC	6 cut(s)	31, 138, 143, 339, 342, 674
SspMI	CTAG	2 cut(s)	198, 1377
StyD4I	CCNGG	4 cut(s)	261, 529, 1124, 1476
StyI	CCWWGG	2 cut(s)	1028, 1250
TaaI	ACNGT	2 cut(s)	53, 1285
TaiI	ACGT	1 cut(s)	1195
TaqI	TCGA	4 cut(s)	16, 62, 1218, 1337
TaqII	GACCGA	1 cut(s)	555
TasI	AATT	9 cut(s)	18, 121, 158, 384, 594, 822, 997, 1236, 1296
TatI	WGTACW	2 cut(s)	833, 854
TauI	GCSGC	2 cut(s)	33, 342
TfiI	GAWTC	1 cut(s)	13
Tru1I	TTAA	9 cut(s)	192, 377, 441, 810, 821, 825, 924, 1070, 1470
Tru9I	TTAA	9 cut(s)	192, 377, 441, 810, 821, 825, 924, 1070, 1470
TscAI	CASTG	1 cut(s)	430
TseFI	GTSAC	1 cut(s)	1368
TseI	GCWGC	2 cut(s)	356, 389
Tsp45I	GTSAC	1 cut(s)	1368
TspDTI	ATGAA	5 cut(s)	633, 683, 767, 947, 1219
TspGWI	ACGGA	1 cut(s)	1200
TspRI	CASTG	1 cut(s)	430
Tth111I	GACNNNGTC	1 cut(s)	942
VpaK11BI	GGWCC	1 cut(s)	667
VspI	ATTAAT	1 cut(s)	825
XapI	RAATTY	3 cut(s)	18, 158, 1296
XceI	RCATGY	2 cut(s)	689, 1324
XcmI	CCANNNNNNNNNTGG	3 cut(s)	475, 506, 716
XmiI	GTMKAC	1 cut(s)	1218
XmnI	GAANNNNTTC	1 cut(s)	228
XspI	CTAG	2 cut(s)	198, 1377
ZrmI	AGTACT	1 cut(s)	835
Zsp2I	ATGCAT	2 cut(s)	691, 888

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.