Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

30508710 .. 30514148

Gene Structure 5439 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG279700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGGTGGGAGATAGAGGCAAGTTGAAGGATTTTTACATTCCGAATTACATACTTGTGGGCGGGGCGGTGCCGAAGATCAAATTTGCACCTGCGTGTCCGGTGATAGTGTTTGTTAATATTAGAAGTGGTGGTCAGCTTGGAGGTGAACTTCTTCTTACATATAGAGCCCTTCTCAATGAGCACCAGGTATTTGACTTGGGACAAACGGCTCCTGACAAGGTGCTTCACAAGCTCTATGTTGCTTTGGAAACACTCAAGGGCAGAGGAGATGTTTTGGCTGCTGATATCCAGAAGAGGTTAAGAATAATTGCTGCAGGTGGAGATGGTACAGCTGGCTGGCTCCTCAGTGTAGTTTCTGATCTTAAACTGCCTAATCCACCTCCAATCGCCACGATGCCATTGGGAACTGGAAATAATGTCCATTTCGCGTTTGGATGGGGAAAGAAAAATCCTGGGACAGACCGACAATCTGTGCAGTATTTCTTGAACCAAGTGAAGACAGCAAAGGAAATGCAAATTGACAGCTGGCACATCATTATGAGAATGAGGACTCCAAAGGAAGGTTCATGTGATCCTATTGCACCTCTGGACCTACCGCATTCTTTGCATGCATTTCATCAGGTCTCCAAAACTGATACACTCAATCTGGAGGGCTTCCAGACATTTCGTGGGGGTTTTTGGAACTACTTTAGCATGGGAATGGATGCTCAAATATCATATGATTTTCATGCTCAGAGAAAGTTGCACGCTGAAAAGTTTAAGAACCAGTTAATTAATCAAAGTACTTACTTCAAGCTTGGATGTACTCAAGGATGGTTCTTTGCTTCTCTAATGCATCCTTCTTCAAGGAATATAGCACATCTGACAAAGATTAAGATCATGAAAAGACAAGGTCATTGGGAGGAGCTTCACATATCTCCCAGCATAAGGTCTATTGTTTGCCTCAATTTACCCAGTTTTTCTGGTGGTCTTGATCCATGGGGGAAGCCAAATAGAAAGAAACTGCGATATCGGGATTTAACTCCTCCATATGTGGATGATGGCCTTCTTGAGATTGTTGGTTTTAGAGATGCCTGGCATGGGCTTGCGTTACTTGCTCCTAAGGGACATGGGACTCGTCTTGCACAAGCACACAGAATACGTTTTGAGTTTTGCAAGGGCGCAGTCGACCATACATTCATGAGAATTGATGGGGAACCATGGAAACAACCTCTTCCAGTTGACGATGACACGGTGGTTGTAGAAATTTCTCACTTTGGCCAAGTAAGCATGCTTGCCACGCCATCGAAGCGATCCAAAAGTATATCTGACCCTGCGTCACCAGCTAGCTCCCATGATGACGATGATGATAGTAGTAATGAGGAAGAATATCCAGAAGATAGTGGAGAGAGAAGGAAGTTTGGTGCATCTGACACATTTAAAATCCCGGACGGGATTGACATTGCTCATTTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007610 GO:0007635 GO:0008150 GO:0009266 GO:0009605 GO:0009628 GO:0010646 GO:0023051 GO:0040011 GO:0042048 GO:0042221 GO:0042330 GO:0043052 GO:0048167 GO:0048168 GO:0048169 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0065007 GO:0065008 GO:0099177

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

485

Amino Acids

54.17

Weight (kDa)

8.6

Isoelectric Point (pI)

35.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	33 - 153	8.9e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	226 - 400	3.7e-36	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	97, 305
Acc36I	ACCTGC	2 cut(s)	97, 305
AccB1I	GGYRCC	1 cut(s)	67
AccI	GTMKAC	1 cut(s)	1167
AccII	CGCG	1 cut(s)	428
AciI	CCGC	3 cut(s)	60, 65, 596
AclWI	GGATC	3 cut(s)	566, 968, 1287
AcoI	YGGCCR	1 cut(s)	1258
AcsI	RAATTY	2 cut(s)	80, 1245
AfaI	GTAC	3 cut(s)	328, 784, 805
AfiI	CCNNNNNNNGG	3 cut(s)	560, 927, 1080
AgsI	TTSAA	4 cut(s)	25, 487, 793, 846
AhdI	GACNNNNNGTC	1 cut(s)	1315
AjnI	CCWGG	3 cut(s)	183, 451, 1073
AjuI	GAANNNNNNNTTGG	2 cut(s)	547, 579
AleI	CACNNNNGTG	1 cut(s)	91
AluBI	AGCT	8 cut(s)	136, 232, 332, 525, 796, 907, 1325, 1329
AluI	AGCT	8 cut(s)	136, 232, 332, 525, 796, 907, 1325, 1329
Alw21I	GWGCWC	1 cut(s)	183
Alw26I	GTCTC	1 cut(s)	628
AlwI	GGATC	3 cut(s)	566, 968, 1287
AoxI	GGCC	2 cut(s)	1042, 1258
ApeKI	GCWGC	2 cut(s)	278, 311
ApoI	RAATTY	2 cut(s)	80, 1245
AseI	ATTAAT	1 cut(s)	774
Asp700I	GAANNNNTTC	1 cut(s)	150
AspLEI	GCGC	1 cut(s)	1163
AspS9I	GGNCC	1 cut(s)	589
AsuC2I	CCSGG	1 cut(s)	1427
AsuHPI	GGTGA	3 cut(s)	112, 155, 1311
AsuNHI	GCTAGC	1 cut(s)	1325
AvaII	GGWCC	1 cut(s)	589
AxyI	CCTNAGG	1 cut(s)	1101
BalI	TGGCCA	1 cut(s)	1260
BanI	GGYRCC	1 cut(s)	67
BanII	GRGCYC	1 cut(s)	169
BarI	GAAGNNNNNNTAC	2 cut(s)	17, 49
BbsI	GAAGAC	1 cut(s)	503
Bbv12I	GWGCWC	1 cut(s)	183
BbvI	GCAGC	2 cut(s)	265, 298
BccI	CCATC	6 cut(s)	317, 429, 807, 1034, 1184, 1291
BceAI	ACGGC	1 cut(s)	222
BciT130I	CCWGG	3 cut(s)	185, 453, 1075
BcnI	CCSGG	1 cut(s)	1427
BcoDI	GTCTC	1 cut(s)	628
BfaI	CTAG	1 cut(s)	1326
BfmI	CTRYAG	1 cut(s)	312
BfuAI	ACCTGC	2 cut(s)	97, 305
BisI	GCNGC	2 cut(s)	279, 312
BlsI	GCNGC	2 cut(s)	280, 313
BmcAI	AGTACT	1 cut(s)	784
Bme1390I	CCNGG	4 cut(s)	185, 453, 1075, 1427
Bme18I	GGWCC	1 cut(s)	589
BmeRI	GACNNNNNGTC	1 cut(s)	1315
BmgT120I	GGNCC	1 cut(s)	589
BmiI	GGNNCC	4 cut(s)	69, 210, 341, 1197
BmrFI	CCNGG	4 cut(s)	185, 453, 1075, 1427
BmrI	ACTGGG	1 cut(s)	948
BmsI	GCATC	5 cut(s)	384, 694, 844, 1060, 1415
BmtI	GCTAGC	1 cut(s)	1329
BmuI	ACTGGG	1 cut(s)	948
BpiI	GAAGAC	1 cut(s)	503
BplI	GAGNNNNNCTC	2 cut(s)	156, 188
BpmI	CTGGAG	1 cut(s)	668
BpuEI	CTTGAG	3 cut(s)	239, 792, 1070
BpuMI	CCSGG	1 cut(s)	1427
BsaBI	GATNNNNATC	1 cut(s)	875
BsaI	GGTCTC	1 cut(s)	628
BsaJI	CCNNGG	3 cut(s)	452, 977, 1199
BsaWI	WCCGGW	1 cut(s)	97
Bsc4I	CCNNNNNNNGG	3 cut(s)	560, 927, 1080
Bse1I	ACTGG	4 cut(s)	412, 766, 954, 1217
Bse21I	CCTNAGG	1 cut(s)	1101
Bse3DI	GCAATG	1 cut(s)	1440
Bse8I	GATNNNNATC	1 cut(s)	875
BseBI	CCWGG	3 cut(s)	185, 453, 1075
BseDI	CCNNGG	3 cut(s)	452, 977, 1199
BseGI	GGATG	6 cut(s)	440, 709, 806, 818, 835, 1042
BseJI	GATNNNNATC	1 cut(s)	875
BseLI	CCNNNNNNNGG	3 cut(s)	560, 927, 1080
BseMI	GCAATG	1 cut(s)	1440
BseMII	CTCAG	2 cut(s)	358, 746
BseNI	ACTGG	4 cut(s)	412, 766, 954, 1217
BseRI	GAGGAG	4 cut(s)	279, 332, 917, 1014
BseXI	GCAGC	2 cut(s)	265, 298
BseYI	CCCAGC	1 cut(s)	920
BsgI	GTGCAG	1 cut(s)	494
Bsh1236I	CGCG	1 cut(s)	428
BshFI	GGCC	2 cut(s)	1044, 1260
BshNI	GGYRCC	1 cut(s)	67
BsiHKAI	GWGCWC	1 cut(s)	183
BsiSI	CCGG	2 cut(s)	98, 1427
BslFI	GGGAC	4 cut(s)	213, 469, 1119, 1126
BslI	CCNNNNNNNGG	3 cut(s)	560, 927, 1080
BsmAI	GTCTC	1 cut(s)	628
BsmFI	GGGAC	4 cut(s)	213, 469, 1119, 1126
BsmI	GAATGC	1 cut(s)	598
BsnI	GGCC	2 cut(s)	1044, 1260
Bso31I	GGTCTC	1 cut(s)	628
Bsp1286I	GDGCHC	2 cut(s)	169, 183
Bsp143I	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
Bsp19I	CCATGG	2 cut(s)	977, 1199
BspACI	CCGC	3 cut(s)	60, 65, 596
BspANI	GGCC	2 cut(s)	1044, 1260
BspCNI	CTCAG	2 cut(s)	357, 745
BspFNI	CGCG	1 cut(s)	428
BspHI	TCATGA	2 cut(s)	879, 1179
BspLI	GGNNCC	4 cut(s)	69, 210, 341, 1197
BspMAI	CTGCAG	1 cut(s)	316
BspMI	ACCTGC	2 cut(s)	97, 305
BspOI	GCTAGC	1 cut(s)	1329
BspPI	GGATC	3 cut(s)	566, 968, 1287
BspT107I	GGYRCC	1 cut(s)	67
BspTNI	GGTCTC	1 cut(s)	628
BsrDI	GCAATG	1 cut(s)	1440
BsrI	ACTGG	4 cut(s)	412, 766, 954, 1217
BssECI	CCNNGG	3 cut(s)	452, 977, 1199
BssMI	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
BssT1I	CCWWGG	2 cut(s)	977, 1199
Bst2UI	CCWGG	3 cut(s)	185, 453, 1075
Bst4CI	ACNGT	1 cut(s)	1234
Bst6I	CTCTTC	2 cut(s)	287, 1218
BstAPI	GCANNNNNTGC	1 cut(s)	604
BstC8I	GCNNGC	9 cut(s)	334, 338, 527, 609, 747, 1086, 1271, 1275, 1327
BstDEI	CTNAG	3 cut(s)	344, 732, 1101
BstDSI	CCRYGG	2 cut(s)	977, 1199
BstF5I	GGATG	6 cut(s)	440, 709, 806, 818, 835, 1042
BstFNI	CGCG	1 cut(s)	428
BstHHI	GCGC	1 cut(s)	1163
BstKTI	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
BstMAI	GTCTC	1 cut(s)	628
BstMBI	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
BstMWI	GCNNNNNNNGC	7 cut(s)	229, 604, 1094, 1266, 1279, 1288, 1322
BstNI	CCWGG	3 cut(s)	185, 453, 1075
BstNSI	RCATGY	2 cut(s)	611, 1273
BstSCI	CCNGG	4 cut(s)	183, 451, 1073, 1425
BstSFI	CTRYAG	1 cut(s)	312
BstUI	CGCG	1 cut(s)	428
BstV1I	GCAGC	2 cut(s)	265, 298
BstV2I	GAAGAC	1 cut(s)	503
Bsu36I	CCTNAGG	1 cut(s)	1101
BsuRI	GGCC	2 cut(s)	1044, 1260
BtgI	CCRYGG	2 cut(s)	977, 1199
BtsCI	GGATG	6 cut(s)	440, 709, 806, 818, 835, 1042
BtsIMutI	CAGTG	1 cut(s)	352
BveI	ACCTGC	2 cut(s)	97, 305
Cac8I	GCNNGC	9 cut(s)	334, 338, 527, 609, 747, 1086, 1271, 1275, 1327
CciI	TCATGA	2 cut(s)	879, 1179
CfoI	GCGC	1 cut(s)	1163
Cfr13I	GGNCC	1 cut(s)	589
CseI	GACGC	1 cut(s)	1305
CsiI	ACCWGGT	1 cut(s)	183
Csp6I	GTAC	3 cut(s)	327, 783, 804
CviQI	GTAC	3 cut(s)	327, 783, 804
DdeI	CTNAG	3 cut(s)	344, 732, 1101
DpnI	GATC	6 cut(s)	77, 360, 573, 878, 975, 1294
DpnII	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
DraI	TTTAAA	1 cut(s)	1420
DriI	GACNNNNNGTC	1 cut(s)	1315
EaeI	YGGCCR	1 cut(s)	1258
Eam1104I	CTCTTC	2 cut(s)	287, 1218
Eam1105I	GACNNNNNGTC	1 cut(s)	1315
EarI	CTCTTC	2 cut(s)	287, 1218
Eco130I	CCWWGG	2 cut(s)	977, 1199
Eco24I	GRGCYC	1 cut(s)	169
Eco31I	GGTCTC	1 cut(s)	628
Eco32I	GATATC	2 cut(s)	286, 1010
Eco47I	GGWCC	1 cut(s)	589
Eco81I	CCTNAGG	1 cut(s)	1101
EcoRII	CCWGG	3 cut(s)	183, 451, 1073
EcoRV	GATATC	2 cut(s)	286, 1010
EcoT14I	CCWWGG	2 cut(s)	977, 1199
EcoT22I	ATGCAT	2 cut(s)	613, 837
EcoT38I	GRGCYC	1 cut(s)	169
ErhI	CCWWGG	2 cut(s)	977, 1199
FaqI	GGGAC	4 cut(s)	213, 469, 1119, 1126
FauI	CCCGC	1 cut(s)	53
FauNDI	CATATG	2 cut(s)	718, 1030
FblI	GTMKAC	1 cut(s)	1167
Fnu4HI	GCNGC	2 cut(s)	279, 312
FokI	GGATG	6 cut(s)	447, 716, 813, 822, 825, 1049
FriOI	GRGCYC	1 cut(s)	169
Fsp4HI	GCNGC	2 cut(s)	279, 312
FspBI	CTAG	1 cut(s)	1326
GlaI	GCGC	1 cut(s)	1162
GluI	GCNGC	2 cut(s)	279, 312
GsaI	CCCAGC	1 cut(s)	924
GsuI	CTGGAG	1 cut(s)	668
HaeIII	GGCC	2 cut(s)	1044, 1260
HapII	CCGG	2 cut(s)	98, 1427
HgaI	GACGC	1 cut(s)	1305
HhaI	GCGC	1 cut(s)	1163
Hin6I	GCGC	1 cut(s)	1161
HinP1I	GCGC	1 cut(s)	1161
HincII	GTYRAC	2 cut(s)	1168, 1222
HindII	GTYRAC	2 cut(s)	1168, 1222
HindIII	AAGCTT	1 cut(s)	794
HinfI	GANTC	2 cut(s)	550, 1114
HpaII	CCGG	2 cut(s)	98, 1427
HphI	GGTGA	3 cut(s)	112, 155, 1311
Hpy166II	GTNNAC	3 cut(s)	146, 1168, 1222
Hpy188I	TCNGA	6 cut(s)	42, 358, 735, 864, 1309, 1411
Hpy8I	GTNNAC	3 cut(s)	146, 1168, 1222
HpyAV	CCTTC	6 cut(s)	19, 179, 554, 849, 1055, 1386
HpyCH4III	ACNGT	1 cut(s)	1234
HpyCH4IV	ACGT	1 cut(s)	1141
HpyF10VI	GCNNNNNNNGC	7 cut(s)	229, 604, 1094, 1266, 1279, 1288, 1322
HpyF3I	CTNAG	3 cut(s)	344, 732, 1101
HpySE526I	ACGT	1 cut(s)	1141
HspAI	GCGC	1 cut(s)	1161
Kzo9I	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
LmnI	GCTCC	5 cut(s)	214, 345, 904, 1102, 1334
Lsp1109I	GCAGC	2 cut(s)	265, 298
LweI	GCATC	5 cut(s)	384, 694, 844, 1060, 1415
MabI	ACCWGGT	1 cut(s)	183
MaeI	CTAG	1 cut(s)	1326
MaeII	ACGT	1 cut(s)	1141
MaeIII	GTNAC	2 cut(s)	1089, 1317
MalI	GATC	6 cut(s)	77, 360, 573, 878, 975, 1294
MboI	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
MboII	GAAGA	8 cut(s)	85, 143, 304, 508, 834, 1205, 1376, 1388
MhlI	GDGCHC	2 cut(s)	169, 183
MlsI	TGGCCA	1 cut(s)	1260
MluCI	AATT	8 cut(s)	43, 80, 306, 516, 771, 946, 1185, 1245
MluNI	TGGCCA	1 cut(s)	1260
MlyI	GAGTC	2 cut(s)	544, 1108
Mox20I	TGGCCA	1 cut(s)	1260
Mph1103I	ATGCAT	2 cut(s)	613, 837
MroXI	GAANNNNTTC	1 cut(s)	150
MscI	TGGCCA	1 cut(s)	1260
MseI	TTAA	9 cut(s)	114, 299, 363, 759, 770, 774, 873, 1019, 1419
MslI	CAYNNNNRTG	4 cut(s)	53, 91, 536, 698
Msp20I	TGGCCA	1 cut(s)	1260
MspA1I	CMGCKG	2 cut(s)	332, 525
MspI	CCGG	2 cut(s)	98, 1427
MspR9I	CCNGG	4 cut(s)	185, 453, 1075, 1427
Mva1269I	GAATGC	1 cut(s)	598
MvaI	CCWGG	3 cut(s)	185, 453, 1075
MvnI	CGCG	1 cut(s)	428
MwoI	GCNNNNNNNGC	7 cut(s)	229, 604, 1094, 1266, 1279, 1288, 1322
NciI	CCSGG	1 cut(s)	1427
NcoI	CCATGG	2 cut(s)	977, 1199
NdeI	CATATG	2 cut(s)	718, 1030
NdeII	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
NheI	GCTAGC	1 cut(s)	1325
NlaIV	GGNNCC	4 cut(s)	69, 210, 341, 1197
NmuCI	GTSAC	1 cut(s)	1317
NsiI	ATGCAT	2 cut(s)	613, 837
NspI	RCATGY	2 cut(s)	611, 1273
OliI	CACNNNNGTG	1 cut(s)	91
PacI	TTAATTAA	1 cut(s)	774
PaeI	GCATGC	2 cut(s)	611, 1273
PagI	TCATGA	2 cut(s)	879, 1179
PaqCI	CACCTGC	2 cut(s)	97, 305
PctI	GAATGC	1 cut(s)	598
PdmI	GAANNNNTTC	1 cut(s)	150
PflFI	GACNNNGTC	1 cut(s)	891
PfoI	TCCNGGA	1 cut(s)	1425
PkrI	GCNGC	2 cut(s)	280, 313
PleI	GAGTC	2 cut(s)	544, 1108
PpsI	GAGTC	2 cut(s)	544, 1108
PshBI	ATTAAT	1 cut(s)	774
Psp6I	CCWGG	3 cut(s)	183, 451, 1073
PspFI	CCCAGC	1 cut(s)	920
PspGI	CCWGG	3 cut(s)	183, 451, 1073
PspN4I	GGNNCC	4 cut(s)	69, 210, 341, 1197
PspPI	GGNCC	1 cut(s)	589
PstI	CTGCAG	1 cut(s)	316
PsyI	GACNNNGTC	1 cut(s)	891
PvuII	CAGCTG	2 cut(s)	332, 525
RsaI	GTAC	3 cut(s)	328, 784, 805
RsaNI	GTAC	3 cut(s)	327, 783, 804
RseI	CAYNNNNRTG	4 cut(s)	53, 91, 536, 698
SalI	GTCGAC	1 cut(s)	1166
SaqAI	TTAA	9 cut(s)	114, 299, 363, 759, 770, 774, 873, 1019, 1419
SatI	GCNGC	2 cut(s)	279, 312
Sau3AI	GATC	6 cut(s)	75, 358, 571, 876, 973, 1292
Sau96I	GGNCC	1 cut(s)	589
ScaI	AGTACT	1 cut(s)	784
SchI	GAGTC	2 cut(s)	544, 1108
ScrFI	CCNGG	4 cut(s)	185, 453, 1075, 1427
SduI	GDGCHC	2 cut(s)	169, 183
SexAI	ACCWGGT	1 cut(s)	183
SfaNI	GCATC	5 cut(s)	384, 694, 844, 1060, 1415
SfcI	CTRYAG	1 cut(s)	312
SinI	GGWCC	1 cut(s)	589
SmiMI	CAYNNNNRTG	4 cut(s)	53, 91, 536, 698
SmlI	CTYRAG	3 cut(s)	254, 807, 1049
SmoI	CTYRAG	3 cut(s)	254, 807, 1049
SphI	GCATGC	2 cut(s)	611, 1273
Sse9I	AATT	8 cut(s)	43, 80, 306, 516, 771, 946, 1185, 1245
SsiI	CCGC	3 cut(s)	60, 65, 596
SspI	AATATT	1 cut(s)	118
SspMI	CTAG	1 cut(s)	1326
StyD4I	CCNGG	4 cut(s)	183, 451, 1073, 1425
StyI	CCWWGG	2 cut(s)	977, 1199
TaaI	ACNGT	1 cut(s)	1234
TaiI	ACGT	1 cut(s)	1144
TaqI	TCGA	2 cut(s)	1167, 1286
TaqII	GACCGA	1 cut(s)	477
TasI	AATT	8 cut(s)	43, 80, 306, 516, 771, 946, 1185, 1245
TatI	WGTACW	2 cut(s)	782, 803
Tru1I	TTAA	9 cut(s)	114, 299, 363, 759, 770, 774, 873, 1019, 1419
Tru9I	TTAA	9 cut(s)	114, 299, 363, 759, 770, 774, 873, 1019, 1419
TscAI	CASTG	1 cut(s)	352
TseFI	GTSAC	1 cut(s)	1317
TseI	GCWGC	2 cut(s)	278, 311
Tsp45I	GTSAC	1 cut(s)	1317
TspDTI	ATGAA	5 cut(s)	555, 605, 716, 896, 1168
TspRI	CASTG	1 cut(s)	352
Tth111I	GACNNNGTC	1 cut(s)	891
VpaK11BI	GGWCC	1 cut(s)	589
VspI	ATTAAT	1 cut(s)	774
XapI	RAATTY	2 cut(s)	80, 1245
XceI	RCATGY	2 cut(s)	611, 1273
XcmI	CCANNNNNNNNNTGG	3 cut(s)	397, 428, 665
XmiI	GTMKAC	1 cut(s)	1167
XmnI	GAANNNNTTC	1 cut(s)	150
XspI	CTAG	1 cut(s)	1326
ZrmI	AGTACT	1 cut(s)	784
Zsp2I	ATGCAT	2 cut(s)	613, 837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.