Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

30757178 .. 30763922

Gene Structure 6745 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033679

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1461 bp

ATGGTGGGCGATAGAGGCAAGTTGAAGGATTTTTACATTCCGAATTACATACTTGCGGGCGGGGCGATGATGCCGAAGATCAAATTTGCGCCTGCGTGTCCGGTGATAGTGTTTGTTAATACTAGAAGTGGTGGTCAGCTTGGAGGTGAACTTCTTCTTACATATAGAGCCCTTCTCAATGAGCACCAGGTATTTGACTTGGGACAAACGGCTCCTGACAAGGTGCTTCACAAGCTCTATGTTGCTTTGGAAACACTCAAGGGCAGCGGAGATGTTTTGGCTGCTGATATCCAGAAGAGGTTAAGAATAATTGCTGCAGGTGGAGATGGTACAGCTGGCTGGCTCCTCAGTGTAGTTTCTGATCTTAAACTGCCTAACCCACCTCCAATTGCCACGATGCCATTGGGAACTGGAAATAATGTCCATTTCGCGTTTGGATGGGGAAAGAAAAATCCTGGGACAGACCGACAATCTGTGCAGTATTTCTTGAACCAAGTGAAGACAGCAAAGGAAATGCAAATTGACAGCTGGCACATCATTATGAGAATGAGGACTCCAAAGGAAGGTTCGTGTGATCCTATTGCACCTCTGGACCTACCGCATTCTTTGCATGCATTTCATCAGGTCCCCAAAACTGATACACTCAATGTGGAGGGCTTCCAGACATTTCGTGGGGGATTTTGGAACTACTTTAGCATGGGAATGGATGCTCAAATATCATATGATTTTCATGCTCAGAGAAAGTTGCACGCTGAAAAGTTTAACAACCAGTTAATTAATCAAAGTACTTACTTCAAGCTTGGATGTACTCAAGGATGGTTCTTTGCTTCTCTAATGCATCCTTCTTCAAGGAATATAGCACATCTGACAAAGATTAAGATCATGAAAAGACAAGGTCATTGGGAGGAGCTCCACATATCTCCCAGCATTAGGTCTATAGTTTGCCTCAATTTACCCAGTTTTTCTGGTGGTCTTGATCCATGGGGGAAGCCAAATAGAAAGAAACTGCGATATCGGGATTTAACTCCTCCATATGTGGATGATGGCCTTCTTGAGATTGTTGGTTTTAGAGATGCCTGGCATGGGCTTGCGTTACTTGCTCCTAAGGGACATGGGACTCGTCTTGCACAAGCACATGGAATACGTTTTGAGTTTTGCAAGGGCGCAGTCGACCATACATTCATGAGAATTGATGGGGAACCATGGAAACAACCTCTTCCAGTTGACGATGACACGGTGGTTGTAGAAATTTCTCACTTTGGCCAAGCATGCATGCTTGCCACGCCATCGAAGCGATCCAAAAGTATATCTGACCCTGCGTCACCAGCTAGTTCCCATGATGACGATGATGATAGTAGTAATGAGGAAGAATATCCAGAAGACAGTGCAGAGAGAAAGAAGTTTGGTGCATCTGACACATTTAAAATCCCGGACGGGATTGACAATGCTCATTTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007610 GO:0007635 GO:0008150 GO:0009266 GO:0009605 GO:0009628 GO:0010646 GO:0023051 GO:0040011 GO:0042048 GO:0042221 GO:0042330 GO:0043052 GO:0048167 GO:0048168 GO:0048169 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0065007 GO:0065008 GO:0099177

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

487

Amino Acids

54.08

Weight (kDa)

7.72

Isoelectric Point (pI)

36.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	34 - 154	3.2e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	227 - 401	4.2e-36	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	308
Acc36I	ACCTGC	1 cut(s)	308
AccI	GTMKAC	1 cut(s)	1170
AccII	CGCG	1 cut(s)	431
AciI	CCGC	4 cut(s)	56, 60, 267, 599
AclWI	GGATC	3 cut(s)	569, 971, 1290
AcoI	YGGCCR	1 cut(s)	1261
AcsI	RAATTY	2 cut(s)	83, 1248
AfaI	GTAC	3 cut(s)	331, 787, 808
AfiI	CCNNNNNNNGG	3 cut(s)	563, 930, 1083
AgsI	TTSAA	4 cut(s)	25, 490, 796, 849
AhdI	GACNNNNNGTC	1 cut(s)	1318
AjnI	CCWGG	3 cut(s)	186, 454, 1076
AjuI	GAANNNNNNNTTGG	2 cut(s)	550, 582
AluBI	AGCT	7 cut(s)	139, 235, 335, 528, 799, 910, 1328
AluI	AGCT	7 cut(s)	139, 235, 335, 528, 799, 910, 1328
Alw21I	GWGCWC	2 cut(s)	186, 912
AlwI	GGATC	3 cut(s)	569, 971, 1290
AoxI	GGCC	2 cut(s)	1045, 1261
ApeKI	GCWGC	3 cut(s)	264, 281, 314
ApoI	RAATTY	2 cut(s)	83, 1248
AseI	ATTAAT	1 cut(s)	777
Asp700I	GAANNNNTTC	1 cut(s)	153
AspLEI	GCGC	2 cut(s)	91, 1166
AspS9I	GGNCC	2 cut(s)	592, 625
AsuC2I	CCSGG	1 cut(s)	1430
AsuHPI	GGTGA	3 cut(s)	115, 158, 1314
AvaII	GGWCC	2 cut(s)	592, 625
AxyI	CCTNAGG	1 cut(s)	1104
BalI	TGGCCA	1 cut(s)	1263
BanII	GRGCYC	2 cut(s)	172, 912
BarI	GAAGNNNNNNTAC	2 cut(s)	17, 49
BbsI	GAAGAC	2 cut(s)	506, 1386
Bbv12I	GWGCWC	2 cut(s)	186, 912
BbvI	GCAGC	3 cut(s)	268, 276, 301
BccI	CCATC	6 cut(s)	320, 432, 810, 1037, 1187, 1294
BceAI	ACGGC	1 cut(s)	225
BciT130I	CCWGG	3 cut(s)	188, 456, 1078
BcnI	CCSGG	1 cut(s)	1430
BfaI	CTAG	2 cut(s)	123, 1329
BfmI	CTRYAG	2 cut(s)	315, 936
BfuAI	ACCTGC	1 cut(s)	308
BisI	GCNGC	3 cut(s)	265, 282, 315
BlsI	GCNGC	3 cut(s)	266, 283, 316
BmcAI	AGTACT	1 cut(s)	787
Bme1390I	CCNGG	4 cut(s)	188, 456, 1078, 1430
Bme18I	GGWCC	2 cut(s)	592, 625
BmeRI	GACNNNNNGTC	1 cut(s)	1318
BmgT120I	GGNCC	2 cut(s)	592, 625
BmiI	GGNNCC	4 cut(s)	213, 344, 627, 1200
BmrFI	CCNGG	4 cut(s)	188, 456, 1078, 1430
BmrI	ACTGGG	1 cut(s)	951
BmsI	GCATC	6 cut(s)	60, 387, 697, 847, 1063, 1418
BmuI	ACTGGG	1 cut(s)	951
BpiI	GAAGAC	2 cut(s)	506, 1386
BplI	GAGNNNNNCTC	2 cut(s)	159, 191
BpuEI	CTTGAG	3 cut(s)	242, 795, 1073
BpuMI	CCSGG	1 cut(s)	1430
BsaBI	GATNNNNATC	1 cut(s)	878
BsaJI	CCNNGG	3 cut(s)	455, 980, 1202
BsaWI	WCCGGW	1 cut(s)	100
Bsc4I	CCNNNNNNNGG	3 cut(s)	563, 930, 1083
Bse1I	ACTGG	4 cut(s)	415, 769, 957, 1220
Bse21I	CCTNAGG	1 cut(s)	1104
Bse8I	GATNNNNATC	1 cut(s)	878
BseBI	CCWGG	3 cut(s)	188, 456, 1078
BseDI	CCNNGG	3 cut(s)	455, 980, 1202
BseGI	GGATG	6 cut(s)	443, 712, 809, 821, 838, 1045
BseJI	GATNNNNATC	1 cut(s)	878
BseLI	CCNNNNNNNGG	3 cut(s)	563, 930, 1083
BseMII	CTCAG	2 cut(s)	361, 749
BseNI	ACTGG	4 cut(s)	415, 769, 957, 1220
BseRI	GAGGAG	3 cut(s)	335, 920, 1017
BseXI	GCAGC	3 cut(s)	268, 276, 301
BseYI	CCCAGC	1 cut(s)	923
BsgI	GTGCAG	2 cut(s)	497, 1407
Bsh1236I	CGCG	1 cut(s)	431
BshFI	GGCC	2 cut(s)	1047, 1263
BsiHKAI	GWGCWC	2 cut(s)	186, 912
BsiSI	CCGG	2 cut(s)	101, 1430
BslFI	GGGAC	5 cut(s)	216, 472, 611, 1122, 1129
BslI	CCNNNNNNNGG	3 cut(s)	563, 930, 1083
BsmFI	GGGAC	5 cut(s)	216, 472, 611, 1122, 1129
BsmI	GAATGC	1 cut(s)	601
BsnI	GGCC	2 cut(s)	1047, 1263
Bsp1286I	GDGCHC	3 cut(s)	172, 186, 912
Bsp143I	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
Bsp19I	CCATGG	2 cut(s)	980, 1202
BspACI	CCGC	4 cut(s)	56, 60, 267, 599
BspANI	GGCC	2 cut(s)	1047, 1263
BspCNI	CTCAG	2 cut(s)	360, 748
BspFNI	CGCG	1 cut(s)	431
BspHI	TCATGA	2 cut(s)	882, 1182
BspLI	GGNNCC	4 cut(s)	213, 344, 627, 1200
BspMAI	CTGCAG	1 cut(s)	319
BspMI	ACCTGC	1 cut(s)	308
BspPI	GGATC	3 cut(s)	569, 971, 1290
BsrI	ACTGG	4 cut(s)	415, 769, 957, 1220
BssECI	CCNNGG	3 cut(s)	455, 980, 1202
BssMI	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
BssT1I	CCWWGG	2 cut(s)	980, 1202
Bst2UI	CCWGG	3 cut(s)	188, 456, 1078
Bst4CI	ACNGT	2 cut(s)	1237, 1385
Bst6I	CTCTTC	2 cut(s)	290, 1221
BstAPI	GCANNNNNTGC	1 cut(s)	607
BstDEI	CTNAG	3 cut(s)	347, 735, 1104
BstDSI	CCRYGG	2 cut(s)	980, 1202
BstF5I	GGATG	6 cut(s)	443, 712, 809, 821, 838, 1045
BstFNI	CGCG	1 cut(s)	431
BstHHI	GCGC	2 cut(s)	91, 1166
BstKTI	GATC	6 cut(s)	81, 364, 577, 882, 979, 1298
BstMBI	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
BstMWI	GCNNNNNNNGC	9 cut(s)	15, 62, 232, 607, 1097, 1269, 1282, 1291, 1325
BstNI	CCWGG	3 cut(s)	188, 456, 1078
BstNSI	RCATGY	3 cut(s)	614, 1272, 1276
BstSCI	CCNGG	4 cut(s)	186, 454, 1076, 1428
BstSFI	CTRYAG	2 cut(s)	315, 936
BstUI	CGCG	1 cut(s)	431
BstV1I	GCAGC	3 cut(s)	268, 276, 301
BstV2I	GAAGAC	2 cut(s)	506, 1386
Bsu36I	CCTNAGG	1 cut(s)	1104
BsuRI	GGCC	2 cut(s)	1047, 1263
BtgI	CCRYGG	2 cut(s)	980, 1202
BtgZI	GCGATG	1 cut(s)	80
BtsCI	GGATG	6 cut(s)	443, 712, 809, 821, 838, 1045
BtsIMutI	CAGTG	2 cut(s)	355, 1390
BveI	ACCTGC	1 cut(s)	308
CciI	TCATGA	2 cut(s)	882, 1182
CfoI	GCGC	2 cut(s)	91, 1166
Cfr13I	GGNCC	2 cut(s)	592, 625
CseI	GACGC	1 cut(s)	1308
CsiI	ACCWGGT	1 cut(s)	186
Csp6I	GTAC	3 cut(s)	330, 786, 807
CviQI	GTAC	3 cut(s)	330, 786, 807
DdeI	CTNAG	3 cut(s)	347, 735, 1104
DpnI	GATC	6 cut(s)	80, 363, 576, 881, 978, 1297
DpnII	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
DraI	TTTAAA	1 cut(s)	1423
DriI	GACNNNNNGTC	1 cut(s)	1318
EaeI	YGGCCR	1 cut(s)	1261
Eam1104I	CTCTTC	2 cut(s)	290, 1221
Eam1105I	GACNNNNNGTC	1 cut(s)	1318
EarI	CTCTTC	2 cut(s)	290, 1221
Ecl136II	GAGCTC	1 cut(s)	910
Eco130I	CCWWGG	2 cut(s)	980, 1202
Eco24I	GRGCYC	2 cut(s)	172, 912
Eco32I	GATATC	2 cut(s)	289, 1013
Eco47I	GGWCC	2 cut(s)	592, 625
Eco53kI	GAGCTC	1 cut(s)	910
Eco81I	CCTNAGG	1 cut(s)	1104
EcoICRI	GAGCTC	1 cut(s)	910
EcoO109I	RGGNCCY	1 cut(s)	625
EcoRII	CCWGG	3 cut(s)	186, 454, 1076
EcoRV	GATATC	2 cut(s)	289, 1013
EcoT14I	CCWWGG	2 cut(s)	980, 1202
EcoT22I	ATGCAT	3 cut(s)	616, 840, 1274
EcoT38I	GRGCYC	2 cut(s)	172, 912
ErhI	CCWWGG	2 cut(s)	980, 1202
FaqI	GGGAC	5 cut(s)	216, 472, 611, 1122, 1129
FauI	CCCGC	2 cut(s)	49, 53
FauNDI	CATATG	2 cut(s)	721, 1033
FblI	GTMKAC	1 cut(s)	1170
Fnu4HI	GCNGC	3 cut(s)	265, 282, 315
FokI	GGATG	6 cut(s)	450, 719, 816, 825, 828, 1052
FriOI	GRGCYC	2 cut(s)	172, 912
Fsp4HI	GCNGC	3 cut(s)	265, 282, 315
FspBI	CTAG	2 cut(s)	123, 1329
GlaI	GCGC	2 cut(s)	90, 1165
GluI	GCNGC	3 cut(s)	265, 282, 315
GsaI	CCCAGC	1 cut(s)	927
HaeIII	GGCC	2 cut(s)	1047, 1263
HapII	CCGG	2 cut(s)	101, 1430
HgaI	GACGC	1 cut(s)	1308
HhaI	GCGC	2 cut(s)	91, 1166
Hin6I	GCGC	2 cut(s)	89, 1164
HinP1I	GCGC	2 cut(s)	89, 1164
HincII	GTYRAC	2 cut(s)	1171, 1225
HindII	GTYRAC	2 cut(s)	1171, 1225
HindIII	AAGCTT	1 cut(s)	797
HinfI	GANTC	2 cut(s)	553, 1117
HpaII	CCGG	2 cut(s)	101, 1430
HphI	GGTGA	3 cut(s)	115, 158, 1314
Hpy166II	GTNNAC	3 cut(s)	149, 1171, 1225
Hpy188I	TCNGA	6 cut(s)	42, 361, 738, 867, 1312, 1414
Hpy8I	GTNNAC	3 cut(s)	149, 1171, 1225
HpyAV	CCTTC	5 cut(s)	19, 182, 557, 852, 1058
HpyCH4III	ACNGT	2 cut(s)	1237, 1385
HpyCH4IV	ACGT	1 cut(s)	1144
HpyF10VI	GCNNNNNNNGC	9 cut(s)	15, 62, 232, 607, 1097, 1269, 1282, 1291, 1325
HpyF3I	CTNAG	3 cut(s)	347, 735, 1104
HpySE526I	ACGT	1 cut(s)	1144
HspAI	GCGC	2 cut(s)	89, 1164
Kzo9I	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
LmnI	GCTCC	5 cut(s)	217, 348, 907, 915, 1105
Lsp1109I	GCAGC	3 cut(s)	268, 276, 301
LweI	GCATC	6 cut(s)	60, 387, 697, 847, 1063, 1418
MabI	ACCWGGT	1 cut(s)	186
MaeI	CTAG	2 cut(s)	123, 1329
MaeII	ACGT	1 cut(s)	1144
MaeIII	GTNAC	2 cut(s)	1092, 1320
MalI	GATC	6 cut(s)	80, 363, 576, 881, 978, 1297
MboI	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
MboII	GAAGA	8 cut(s)	88, 146, 307, 511, 837, 1208, 1379, 1391
MfeI	CAATTG	1 cut(s)	387
MhlI	GDGCHC	3 cut(s)	172, 186, 912
MlsI	TGGCCA	1 cut(s)	1263
MluCI	AATT	9 cut(s)	43, 83, 309, 387, 519, 774, 949, 1188, 1248
MluNI	TGGCCA	1 cut(s)	1263
MlyI	GAGTC	2 cut(s)	547, 1111
Mox20I	TGGCCA	1 cut(s)	1263
Mph1103I	ATGCAT	3 cut(s)	616, 840, 1274
MroXI	GAANNNNTTC	1 cut(s)	153
MscI	TGGCCA	1 cut(s)	1263
MseI	TTAA	9 cut(s)	117, 302, 366, 762, 773, 777, 876, 1022, 1422
MslI	CAYNNNNRTG	2 cut(s)	539, 701
Msp20I	TGGCCA	1 cut(s)	1263
MspA1I	CMGCKG	3 cut(s)	267, 335, 528
MspI	CCGG	2 cut(s)	101, 1430
MspR9I	CCNGG	4 cut(s)	188, 456, 1078, 1430
MunI	CAATTG	1 cut(s)	387
Mva1269I	GAATGC	1 cut(s)	601
MvaI	CCWGG	3 cut(s)	188, 456, 1078
MvnI	CGCG	1 cut(s)	431
MwoI	GCNNNNNNNGC	9 cut(s)	15, 62, 232, 607, 1097, 1269, 1282, 1291, 1325
NciI	CCSGG	1 cut(s)	1430
NcoI	CCATGG	2 cut(s)	980, 1202
NdeI	CATATG	2 cut(s)	721, 1033
NdeII	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
NlaIV	GGNNCC	4 cut(s)	213, 344, 627, 1200
NmuCI	GTSAC	1 cut(s)	1320
NsiI	ATGCAT	3 cut(s)	616, 840, 1274
NspI	RCATGY	3 cut(s)	614, 1272, 1276
PacI	TTAATTAA	1 cut(s)	777
PaeI	GCATGC	3 cut(s)	614, 1272, 1276
PagI	TCATGA	2 cut(s)	882, 1182
PaqCI	CACCTGC	1 cut(s)	308
PctI	GAATGC	1 cut(s)	601
PdmI	GAANNNNTTC	1 cut(s)	153
PflFI	GACNNNGTC	1 cut(s)	894
PfoI	TCCNGGA	1 cut(s)	1428
PkrI	GCNGC	3 cut(s)	266, 283, 316
PleI	GAGTC	2 cut(s)	547, 1111
PpsI	GAGTC	2 cut(s)	547, 1111
PpuMI	RGGWCCY	1 cut(s)	625
PshBI	ATTAAT	1 cut(s)	777
Psp124BI	GAGCTC	1 cut(s)	912
Psp5II	RGGWCCY	1 cut(s)	625
Psp6I	CCWGG	3 cut(s)	186, 454, 1076
PspFI	CCCAGC	1 cut(s)	923
PspGI	CCWGG	3 cut(s)	186, 454, 1076
PspN4I	GGNNCC	4 cut(s)	213, 344, 627, 1200
PspPI	GGNCC	2 cut(s)	592, 625
PspPPI	RGGWCCY	1 cut(s)	625
PstI	CTGCAG	1 cut(s)	319
PsyI	GACNNNGTC	1 cut(s)	894
PvuII	CAGCTG	2 cut(s)	335, 528
RsaI	GTAC	3 cut(s)	331, 787, 808
RsaNI	GTAC	3 cut(s)	330, 786, 807
RseI	CAYNNNNRTG	2 cut(s)	539, 701
SacI	GAGCTC	1 cut(s)	912
SalI	GTCGAC	1 cut(s)	1169
SaqAI	TTAA	9 cut(s)	117, 302, 366, 762, 773, 777, 876, 1022, 1422
SatI	GCNGC	3 cut(s)	265, 282, 315
Sau3AI	GATC	6 cut(s)	78, 361, 574, 879, 976, 1295
Sau96I	GGNCC	2 cut(s)	592, 625
ScaI	AGTACT	1 cut(s)	787
SchI	GAGTC	2 cut(s)	547, 1111
ScrFI	CCNGG	4 cut(s)	188, 456, 1078, 1430
SduI	GDGCHC	3 cut(s)	172, 186, 912
SexAI	ACCWGGT	1 cut(s)	186
SfaNI	GCATC	6 cut(s)	60, 387, 697, 847, 1063, 1418
SfcI	CTRYAG	2 cut(s)	315, 936
SinI	GGWCC	2 cut(s)	592, 625
SmiMI	CAYNNNNRTG	2 cut(s)	539, 701
SmlI	CTYRAG	3 cut(s)	257, 810, 1052
SmoI	CTYRAG	3 cut(s)	257, 810, 1052
SphI	GCATGC	3 cut(s)	614, 1272, 1276
Sse9I	AATT	9 cut(s)	43, 83, 309, 387, 519, 774, 949, 1188, 1248
SsiI	CCGC	4 cut(s)	56, 60, 267, 599
SspMI	CTAG	2 cut(s)	123, 1329
SstI	GAGCTC	1 cut(s)	912
StyD4I	CCNGG	4 cut(s)	186, 454, 1076, 1428
StyI	CCWWGG	2 cut(s)	980, 1202
TaaI	ACNGT	2 cut(s)	1237, 1385
TaiI	ACGT	1 cut(s)	1147
TaqI	TCGA	2 cut(s)	1170, 1289
TaqII	GACCGA	1 cut(s)	480
TasI	AATT	9 cut(s)	43, 83, 309, 387, 519, 774, 949, 1188, 1248
TatI	WGTACW	2 cut(s)	785, 806
Tru1I	TTAA	9 cut(s)	117, 302, 366, 762, 773, 777, 876, 1022, 1422
Tru9I	TTAA	9 cut(s)	117, 302, 366, 762, 773, 777, 876, 1022, 1422
TscAI	CASTG	2 cut(s)	355, 1390
TseFI	GTSAC	1 cut(s)	1320
TseI	GCWGC	3 cut(s)	264, 281, 314
Tsp45I	GTSAC	1 cut(s)	1320
TspDTI	ATGAA	4 cut(s)	608, 719, 899, 1171
TspRI	CASTG	2 cut(s)	355, 1390
Tth111I	GACNNNGTC	1 cut(s)	894
VpaK11BI	GGWCC	2 cut(s)	592, 625
VspI	ATTAAT	1 cut(s)	777
XapI	RAATTY	2 cut(s)	83, 1248
XceI	RCATGY	3 cut(s)	614, 1272, 1276
XcmI	CCANNNNNNNNNTGG	3 cut(s)	400, 431, 668
XmiI	GTMKAC	1 cut(s)	1170
XmnI	GAANNNNTTC	1 cut(s)	153
XspI	CTAG	2 cut(s)	123, 1329
ZrmI	AGTACT	1 cut(s)	787
Zsp2I	ATGCAT	3 cut(s)	616, 840, 1274

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.