Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

31695002 .. 31700334

Gene Structure 5333 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G022720.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGGAGTTCCGGAATCGAATTTCCGGTTGCCGCTTGCGAGACTCTATACACGGTCTCGTTTCGAGGAGTTGGGAGGCAATGGTGGGAGATAGAGGCAAGTTGAAGGATTTTTACATTCCGAATTACATACTTGTGGGCGGGGCGGTGCCGAAGATCAAATTTGCACCTGCGTGTCCGGTGATAGTGTTTGTTAATACTAGAAGTGGTGGTCAGCTTGGAGGTGAACTTGTTCTTACATATAGAGCCCTTCTCAATGAGCACCAGGTATTTGACTTGGGACAAACGGCTCCTGACAAGGTGCTTCACAAGCTCTATGTTGCTTTGGAAACACTCAAGGGCGGCGGAGATGTTTTGGCTGCTGATATCCAGAAGAGGTTAAGAATAATTGCTGCAGGTGGAGATGGTACAGCTGGCTGGCTCCTCAGTGTAGTTTCTGATCTTAAACTGCCTAACCCACCTCCAATCGCCACGATGCCATTGGGAACTGGAAATAATGTCCACTTCGCGTTTGGATGGGGAAAGAAAAATCCTGGGACAGACCGACAATCTGTGCAGTATTTCTTGAACCAAGTGAAGACAGCAAAGGAAATGCAAATTGACAGTCTTTTCTTTCACCTTTTCTGTATATCTCAGCTGGCACATCATTATGAGAATGAGGACTCCAAAGGAGGTTCATGTGATCCTATTGCACCTCTGGACCTACCGCATTCTTTGCATGCATTTCATCAGGTCTCCAAAACTGATACACTCAATCTGGAGGGCTTCCAGACATTTCGTGGGGGATTTTGGAACTACTTTAGCATGGGAATGGATGCTCAAATATCATATGATTTTCATGCTCAGAGAAAGTTGCACGCTGAAAAGTTTAACAACCAGTTAATTAATCAAAGTACTTACTTCAAGCTTGGATGTACTCAAGGATGGTTCTTTGCTTCTCTAATGCATCCTTCTTCAAGGAATATAGCACATCTGACAAAGATTAAGATCATGAAAAGACAAGGTCATTGGGAGGAGCTTCACATATCTCCCAGCATTAGGTCTATTGTTTGCCTCAATTTACCCAGTTTTTCTGGTGGTCTTGATCCATGGGGGAAGCCAAATAGAAAGAAACTGCGATATCGGGATTTAACTCCTCCATATGTGGATGATGGCCTTCTTGAGATTGTTGGCTTTAGAGATGCCTGGCATGGGCTTGCGTTACTTGCTCCTAAGGGACATGGGACTCGTCTTGCACAAGCACACGGAATACGTTTTGAGTTTTGCAAGGGCGCAGTCGACCATACATTCATGAGAATTGATGGGGAACCATGGAAACAACCTCTTCCAGTTGACGATGACACGGTGGTTGTAGAAATTTCTCACTTTGGCCAAGTAAGCATACTTGCCACGCCATCGAAGCGATCCAAAAGTATATCTGACCCTGCGTCACCAGCTAGCTCCCATGATGACGATGATGATAGTAGTAATGAGGAAGAATATCCAGAAGATAGTGGAGAGAGAAGGAAGTTTGGTGCATCTGACACATTTAAAATCCCGGACGGGATTGACATTGCTCATTTCAATTCAAAATTCGATAACAAAGGCTGTAATAGCAAAATGTTTTGGTGGAAACACTACAAGGAAAAGAGAAATGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007610 GO:0007635 GO:0008150 GO:0009266 GO:0009605 GO:0009628 GO:0010646 GO:0023051 GO:0040011 GO:0042048 GO:0042221 GO:0042330 GO:0043052 GO:0048167 GO:0048168 GO:0048169 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0065007 GO:0065008 GO:0099177

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

545

Amino Acids

61.05

Weight (kDa)

8.45

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	59 - 179	6.6e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	262 - 436	5.3e-36	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	175, 383
Acc36I	ACCTGC	2 cut(s)	175, 383
AccB1I	GGYRCC	1 cut(s)	145
AccI	GTMKAC	1 cut(s)	1275
AccII	CGCG	1 cut(s)	506
AccIII	TCCGGA	1 cut(s)	9
AciI	CCGC	6 cut(s)	31, 138, 143, 339, 342, 704
AclWI	GGATC	3 cut(s)	674, 1076, 1395
AcoI	YGGCCR	1 cut(s)	1366
AcsI	RAATTY	4 cut(s)	18, 158, 1353, 1568
AfaI	GTAC	3 cut(s)	406, 892, 913
AfiI	CCNNNNNNNGG	2 cut(s)	1035, 1188
AgsI	TTSAA	6 cut(s)	103, 565, 901, 954, 1561, 1566
AhdI	GACNNNNNGTC	1 cut(s)	1423
AjnI	CCWGG	3 cut(s)	261, 529, 1181
AleI	CACNNNNGTG	1 cut(s)	169
AluBI	AGCT	8 cut(s)	214, 310, 410, 634, 904, 1015, 1433, 1437
AluI	AGCT	8 cut(s)	214, 310, 410, 634, 904, 1015, 1433, 1437
Alw21I	GWGCWC	1 cut(s)	261
Alw26I	GTCTC	3 cut(s)	33, 59, 736
AlwI	GGATC	3 cut(s)	674, 1076, 1395
Aor13HI	TCCGGA	1 cut(s)	9
AoxI	GGCC	2 cut(s)	1150, 1366
ApeKI	GCWGC	2 cut(s)	356, 389
ApoI	RAATTY	4 cut(s)	18, 158, 1353, 1568
ArsI	GACNNNNNNTTYG	2 cut(s)	586, 618
AseI	ATTAAT	1 cut(s)	882
Asp700I	GAANNNNTTC	1 cut(s)	228
AspLEI	GCGC	1 cut(s)	1271
AspS9I	GGNCC	1 cut(s)	697
AsuC2I	CCSGG	1 cut(s)	1535
AsuHPI	GGTGA	4 cut(s)	190, 233, 605, 1419
AsuNHI	GCTAGC	1 cut(s)	1433
AvaII	GGWCC	1 cut(s)	697
AxyI	CCTNAGG	1 cut(s)	1209
BalI	TGGCCA	1 cut(s)	1368
BanI	GGYRCC	1 cut(s)	145
BanII	GRGCYC	1 cut(s)	247
BarI	GAAGNNNNNNTAC	2 cut(s)	95, 127
BbsI	GAAGAC	1 cut(s)	581
Bbv12I	GWGCWC	1 cut(s)	261
BbvI	GCAGC	2 cut(s)	343, 376
BccI	CCATC	6 cut(s)	395, 507, 915, 1142, 1292, 1399
BceAI	ACGGC	1 cut(s)	300
BciT130I	CCWGG	3 cut(s)	263, 531, 1183
BcnI	CCSGG	1 cut(s)	1535
BcoDI	GTCTC	3 cut(s)	33, 59, 736
BfaI	CTAG	3 cut(s)	198, 1434, 1636
BfmI	CTRYAG	1 cut(s)	390
BfuAI	ACCTGC	2 cut(s)	175, 383
BisI	GCNGC	4 cut(s)	31, 340, 357, 390
BlsI	GCNGC	4 cut(s)	32, 341, 358, 391
BmcAI	AGTACT	1 cut(s)	892
Bme1390I	CCNGG	4 cut(s)	263, 531, 1183, 1535
Bme18I	GGWCC	1 cut(s)	697
BmeRI	GACNNNNNGTC	1 cut(s)	1423
BmgT120I	GGNCC	1 cut(s)	697
BmiI	GGNNCC	4 cut(s)	147, 288, 419, 1305
BmrFI	CCNGG	4 cut(s)	263, 531, 1183, 1535
BmrI	ACTGGG	1 cut(s)	1056
BmsI	GCATC	5 cut(s)	462, 802, 952, 1168, 1523
BmtI	GCTAGC	1 cut(s)	1437
BmuI	ACTGGG	1 cut(s)	1056
BpiI	GAAGAC	1 cut(s)	581
BplI	GAGNNNNNCTC	2 cut(s)	234, 266
BpmI	CTGGAG	1 cut(s)	776
BpuEI	CTTGAG	3 cut(s)	317, 900, 1178
BpuMI	CCSGG	1 cut(s)	1535
BsaBI	GATNNNNATC	1 cut(s)	983
BsaI	GGTCTC	2 cut(s)	59, 736
BsaJI	CCNNGG	3 cut(s)	530, 1085, 1307
BsaWI	WCCGGW	3 cut(s)	9, 23, 175
Bsc4I	CCNNNNNNNGG	2 cut(s)	1035, 1188
Bse1I	ACTGG	4 cut(s)	490, 874, 1062, 1325
Bse21I	CCTNAGG	1 cut(s)	1209
Bse3DI	GCAATG	2 cut(s)	84, 1548
Bse8I	GATNNNNATC	1 cut(s)	983
BseAI	TCCGGA	1 cut(s)	9
BseBI	CCWGG	3 cut(s)	263, 531, 1183
BseDI	CCNNGG	3 cut(s)	530, 1085, 1307
BseGI	GGATG	6 cut(s)	518, 817, 914, 926, 943, 1150
BseJI	GATNNNNATC	1 cut(s)	983
BseLI	CCNNNNNNNGG	2 cut(s)	1035, 1188
BseMI	GCAATG	2 cut(s)	84, 1548
BseMII	CTCAG	3 cut(s)	436, 644, 854
BseNI	ACTGG	4 cut(s)	490, 874, 1062, 1325
BseRI	GAGGAG	4 cut(s)	79, 410, 1025, 1122
BseXI	GCAGC	2 cut(s)	343, 376
BseYI	CCCAGC	1 cut(s)	1028
BsgI	GTGCAG	1 cut(s)	572
Bsh1236I	CGCG	1 cut(s)	506
BshFI	GGCC	2 cut(s)	1152, 1368
BshNI	GGYRCC	1 cut(s)	145
BsiHKAI	GWGCWC	1 cut(s)	261
BsiSI	CCGG	4 cut(s)	10, 24, 176, 1535
BslFI	GGGAC	4 cut(s)	291, 547, 1227, 1234
BslI	CCNNNNNNNGG	2 cut(s)	1035, 1188
BsmAI	GTCTC	3 cut(s)	33, 59, 736
BsmFI	GGGAC	4 cut(s)	291, 547, 1227, 1234
BsmI	GAATGC	1 cut(s)	706
BsnI	GGCC	2 cut(s)	1152, 1368
Bso31I	GGTCTC	2 cut(s)	59, 736
Bsp1286I	GDGCHC	2 cut(s)	247, 261
Bsp13I	TCCGGA	1 cut(s)	9
Bsp143I	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
Bsp19I	CCATGG	2 cut(s)	1085, 1307
BspACI	CCGC	6 cut(s)	31, 138, 143, 339, 342, 704
BspANI	GGCC	2 cut(s)	1152, 1368
BspCNI	CTCAG	3 cut(s)	435, 643, 853
BspEI	TCCGGA	1 cut(s)	9
BspFNI	CGCG	1 cut(s)	506
BspHI	TCATGA	2 cut(s)	987, 1287
BspLI	GGNNCC	4 cut(s)	147, 288, 419, 1305
BspMAI	CTGCAG	1 cut(s)	394
BspMI	ACCTGC	2 cut(s)	175, 383
BspOI	GCTAGC	1 cut(s)	1437
BspPI	GGATC	3 cut(s)	674, 1076, 1395
BspT107I	GGYRCC	1 cut(s)	145
BspTNI	GGTCTC	2 cut(s)	59, 736
BsrDI	GCAATG	2 cut(s)	84, 1548
BsrI	ACTGG	4 cut(s)	490, 874, 1062, 1325
BssECI	CCNNGG	3 cut(s)	530, 1085, 1307
BssMI	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
BssT1I	CCWWGG	2 cut(s)	1085, 1307
Bst2UI	CCWGG	3 cut(s)	263, 531, 1183
Bst4CI	ACNGT	3 cut(s)	53, 602, 1342
Bst6I	CTCTTC	2 cut(s)	365, 1326
BstAPI	GCANNNNNTGC	1 cut(s)	712
BstC8I	GCNNGC	8 cut(s)	35, 412, 416, 636, 717, 855, 1194, 1435
BstDEI	CTNAG	4 cut(s)	422, 630, 840, 1209
BstDSI	CCRYGG	2 cut(s)	1085, 1307
BstF5I	GGATG	6 cut(s)	518, 817, 914, 926, 943, 1150
BstFNI	CGCG	1 cut(s)	506
BstHHI	GCGC	1 cut(s)	1271
BstKTI	GATC	6 cut(s)	156, 439, 682, 987, 1084, 1403
BstMAI	GTCTC	3 cut(s)	33, 59, 736
BstMBI	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
BstMWI	GCNNNNNNNGC	7 cut(s)	307, 712, 1202, 1374, 1396, 1430, 1590
BstNI	CCWGG	3 cut(s)	263, 531, 1183
BstNSI	RCATGY	1 cut(s)	719
BstSCI	CCNGG	4 cut(s)	261, 529, 1181, 1533
BstSFI	CTRYAG	1 cut(s)	390
BstUI	CGCG	1 cut(s)	506
BstV1I	GCAGC	2 cut(s)	343, 376
BstV2I	GAAGAC	1 cut(s)	581
Bsu36I	CCTNAGG	1 cut(s)	1209
BsuRI	GGCC	2 cut(s)	1152, 1368
BtgI	CCRYGG	2 cut(s)	1085, 1307
BtsCI	GGATG	6 cut(s)	518, 817, 914, 926, 943, 1150
BtsIMutI	CAGTG	1 cut(s)	430
BveI	ACCTGC	2 cut(s)	175, 383
Cac8I	GCNNGC	8 cut(s)	35, 412, 416, 636, 717, 855, 1194, 1435
CciI	TCATGA	2 cut(s)	987, 1287
CfoI	GCGC	1 cut(s)	1271
Cfr13I	GGNCC	1 cut(s)	697
CseI	GACGC	1 cut(s)	1413
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	3 cut(s)	405, 891, 912
CviQI	GTAC	3 cut(s)	405, 891, 912
DdeI	CTNAG	4 cut(s)	422, 630, 840, 1209
DpnI	GATC	6 cut(s)	155, 438, 681, 986, 1083, 1402
DpnII	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
DraI	TTTAAA	1 cut(s)	1528
DriI	GACNNNNNGTC	1 cut(s)	1423
EaeI	YGGCCR	1 cut(s)	1366
Eam1104I	CTCTTC	2 cut(s)	365, 1326
Eam1105I	GACNNNNNGTC	1 cut(s)	1423
EarI	CTCTTC	2 cut(s)	365, 1326
EciI	GGCGGA	1 cut(s)	357
Eco130I	CCWWGG	2 cut(s)	1085, 1307
Eco24I	GRGCYC	1 cut(s)	247
Eco31I	GGTCTC	2 cut(s)	59, 736
Eco32I	GATATC	2 cut(s)	364, 1118
Eco47I	GGWCC	1 cut(s)	697
Eco81I	CCTNAGG	1 cut(s)	1209
EcoRII	CCWGG	3 cut(s)	261, 529, 1181
EcoRV	GATATC	2 cut(s)	364, 1118
EcoT14I	CCWWGG	2 cut(s)	1085, 1307
EcoT22I	ATGCAT	2 cut(s)	721, 945
EcoT38I	GRGCYC	1 cut(s)	247
ErhI	CCWWGG	2 cut(s)	1085, 1307
FaqI	GGGAC	4 cut(s)	291, 547, 1227, 1234
FauI	CCCGC	1 cut(s)	131
FauNDI	CATATG	2 cut(s)	826, 1138
FblI	GTMKAC	1 cut(s)	1275
Fnu4HI	GCNGC	4 cut(s)	31, 340, 357, 390
FokI	GGATG	6 cut(s)	525, 824, 921, 930, 933, 1157
FriOI	GRGCYC	1 cut(s)	247
Fsp4HI	GCNGC	4 cut(s)	31, 340, 357, 390
FspBI	CTAG	3 cut(s)	198, 1434, 1636
GlaI	GCGC	1 cut(s)	1270
GluI	GCNGC	4 cut(s)	31, 340, 357, 390
GsaI	CCCAGC	1 cut(s)	1032
GsuI	CTGGAG	1 cut(s)	776
HaeIII	GGCC	2 cut(s)	1152, 1368
HapII	CCGG	4 cut(s)	10, 24, 176, 1535
HgaI	GACGC	1 cut(s)	1413
HhaI	GCGC	1 cut(s)	1271
Hin6I	GCGC	1 cut(s)	1269
HinP1I	GCGC	1 cut(s)	1269
HincII	GTYRAC	2 cut(s)	1276, 1330
HindII	GTYRAC	2 cut(s)	1276, 1330
HindIII	AAGCTT	1 cut(s)	902
HinfI	GANTC	4 cut(s)	13, 41, 659, 1222
HpaII	CCGG	4 cut(s)	10, 24, 176, 1535
HphI	GGTGA	4 cut(s)	190, 233, 605, 1419
Hpy166II	GTNNAC	4 cut(s)	224, 499, 1276, 1330
Hpy188I	TCNGA	6 cut(s)	120, 436, 843, 972, 1417, 1519
Hpy8I	GTNNAC	4 cut(s)	224, 499, 1276, 1330
HpyAV	CCTTC	5 cut(s)	97, 257, 957, 1163, 1494
HpyCH4III	ACNGT	3 cut(s)	53, 602, 1342
HpyCH4IV	ACGT	1 cut(s)	1249
HpyF10VI	GCNNNNNNNGC	7 cut(s)	307, 712, 1202, 1374, 1396, 1430, 1590
HpyF3I	CTNAG	4 cut(s)	422, 630, 840, 1209
HpySE526I	ACGT	1 cut(s)	1249
HspAI	GCGC	1 cut(s)	1269
Kpn2I	TCCGGA	1 cut(s)	9
Kzo9I	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
LmnI	GCTCC	5 cut(s)	292, 423, 1012, 1210, 1442
Lsp1109I	GCAGC	2 cut(s)	343, 376
LweI	GCATC	5 cut(s)	462, 802, 952, 1168, 1523
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	3 cut(s)	198, 1434, 1636
MaeII	ACGT	1 cut(s)	1249
MaeIII	GTNAC	2 cut(s)	1197, 1425
MalI	GATC	6 cut(s)	155, 438, 681, 986, 1083, 1402
MboI	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
MboII	GAAGA	7 cut(s)	163, 382, 586, 942, 1313, 1484, 1496
MhlI	GDGCHC	2 cut(s)	247, 261
MlsI	TGGCCA	1 cut(s)	1368
MluNI	TGGCCA	1 cut(s)	1368
MlyI	GAGTC	3 cut(s)	35, 653, 1216
Mox20I	TGGCCA	1 cut(s)	1368
Mph1103I	ATGCAT	2 cut(s)	721, 945
MroI	TCCGGA	1 cut(s)	9
MroXI	GAANNNNTTC	1 cut(s)	228
MscI	TGGCCA	1 cut(s)	1368
MseI	TTAA	9 cut(s)	192, 377, 441, 867, 878, 882, 981, 1127, 1527
MslI	CAYNNNNRTG	4 cut(s)	131, 169, 645, 806
Msp20I	TGGCCA	1 cut(s)	1368
MspA1I	CMGCKG	2 cut(s)	410, 634
MspI	CCGG	4 cut(s)	10, 24, 176, 1535
MspR9I	CCNGG	4 cut(s)	263, 531, 1183, 1535
Mva1269I	GAATGC	1 cut(s)	706
MvaI	CCWGG	3 cut(s)	263, 531, 1183
MvnI	CGCG	1 cut(s)	506
MwoI	GCNNNNNNNGC	7 cut(s)	307, 712, 1202, 1374, 1396, 1430, 1590
NciI	CCSGG	1 cut(s)	1535
NcoI	CCATGG	2 cut(s)	1085, 1307
NdeI	CATATG	2 cut(s)	826, 1138
NdeII	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
NheI	GCTAGC	1 cut(s)	1433
NlaIV	GGNNCC	4 cut(s)	147, 288, 419, 1305
NmuCI	GTSAC	1 cut(s)	1425
NsiI	ATGCAT	2 cut(s)	721, 945
NspI	RCATGY	1 cut(s)	719
OliI	CACNNNNGTG	1 cut(s)	169
PacI	TTAATTAA	1 cut(s)	882
PaeI	GCATGC	1 cut(s)	719
PagI	TCATGA	2 cut(s)	987, 1287
PaqCI	CACCTGC	2 cut(s)	175, 383
PctI	GAATGC	1 cut(s)	706
PdmI	GAANNNNTTC	1 cut(s)	228
PfeI	GAWTC	1 cut(s)	13
PflFI	GACNNNGTC	1 cut(s)	999
PfoI	TCCNGGA	1 cut(s)	1533
PkrI	GCNGC	4 cut(s)	32, 341, 358, 391
PleI	GAGTC	3 cut(s)	35, 653, 1216
PpsI	GAGTC	3 cut(s)	35, 653, 1216
PshBI	ATTAAT	1 cut(s)	882
Psp6I	CCWGG	3 cut(s)	261, 529, 1181
PspFI	CCCAGC	1 cut(s)	1028
PspGI	CCWGG	3 cut(s)	261, 529, 1181
PspN4I	GGNNCC	4 cut(s)	147, 288, 419, 1305
PspPI	GGNCC	1 cut(s)	697
PstI	CTGCAG	1 cut(s)	394
PsyI	GACNNNGTC	1 cut(s)	999
PvuII	CAGCTG	2 cut(s)	410, 634
RsaI	GTAC	3 cut(s)	406, 892, 913
RsaNI	GTAC	3 cut(s)	405, 891, 912
RseI	CAYNNNNRTG	4 cut(s)	131, 169, 645, 806
SalI	GTCGAC	1 cut(s)	1274
SaqAI	TTAA	9 cut(s)	192, 377, 441, 867, 878, 882, 981, 1127, 1527
SatI	GCNGC	4 cut(s)	31, 340, 357, 390
Sau3AI	GATC	6 cut(s)	153, 436, 679, 984, 1081, 1400
Sau96I	GGNCC	1 cut(s)	697
ScaI	AGTACT	1 cut(s)	892
SchI	GAGTC	3 cut(s)	35, 653, 1216
ScrFI	CCNGG	4 cut(s)	263, 531, 1183, 1535
SduI	GDGCHC	2 cut(s)	247, 261
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	5 cut(s)	462, 802, 952, 1168, 1523
SfcI	CTRYAG	1 cut(s)	390
SinI	GGWCC	1 cut(s)	697
SmiMI	CAYNNNNRTG	4 cut(s)	131, 169, 645, 806
SmlI	CTYRAG	3 cut(s)	332, 915, 1157
SmoI	CTYRAG	3 cut(s)	332, 915, 1157
SphI	GCATGC	1 cut(s)	719
SsiI	CCGC	6 cut(s)	31, 138, 143, 339, 342, 704
SspMI	CTAG	3 cut(s)	198, 1434, 1636
StyD4I	CCNGG	4 cut(s)	261, 529, 1181, 1533
StyI	CCWWGG	2 cut(s)	1085, 1307
TaaI	ACNGT	3 cut(s)	53, 602, 1342
TaiI	ACGT	1 cut(s)	1252
TaqI	TCGA	5 cut(s)	16, 62, 1275, 1394, 1572
TaqII	GACCGA	1 cut(s)	555
TatI	WGTACW	2 cut(s)	890, 911
TauI	GCSGC	2 cut(s)	33, 342
TfiI	GAWTC	1 cut(s)	13
Tru1I	TTAA	9 cut(s)	192, 377, 441, 867, 878, 882, 981, 1127, 1527
Tru9I	TTAA	9 cut(s)	192, 377, 441, 867, 878, 882, 981, 1127, 1527
TscAI	CASTG	1 cut(s)	430
TseFI	GTSAC	1 cut(s)	1425
TseI	GCWGC	2 cut(s)	356, 389
Tsp45I	GTSAC	1 cut(s)	1425
TspDTI	ATGAA	5 cut(s)	663, 713, 824, 1004, 1276
TspGWI	ACGGA	1 cut(s)	1257
TspRI	CASTG	1 cut(s)	430
Tth111I	GACNNNGTC	1 cut(s)	999
VpaK11BI	GGWCC	1 cut(s)	697
VspI	ATTAAT	1 cut(s)	882
XapI	RAATTY	4 cut(s)	18, 158, 1353, 1568
XceI	RCATGY	1 cut(s)	719
XcmI	CCANNNNNNNNNTGG	3 cut(s)	475, 506, 773
XmiI	GTMKAC	1 cut(s)	1275
XmnI	GAANNNNTTC	1 cut(s)	228
XspI	CTAG	3 cut(s)	198, 1434, 1636
ZrmI	AGTACT	1 cut(s)	892
Zsp2I	ATGCAT	2 cut(s)	721, 945

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.