Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Forward (+)

73709415 .. 73714575

Gene Structure 5161 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021281

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGAATGAATTACCAGACTTATTCTCTTCGTCTTCCGAATCAAAGTTTAGCTGCTCAGATTGGTTAATGCTACAGCCTCATAGCTCCCACAGGTTTAGTCTGAGATGGCCAAGTCCAGCTCTGGATTCTAATGAGCCCGAGTGTCCAGTTTTGGTGTTTATCAACTCCAAAAGTGGTGGTCAGCTCGGTGGCAATCTTCTTGTGACGTATCGTCAGCTCCTCAATGACTGGCAGGTTTTTGATGTGGGGGAAGAGGCTCCGGATAAGGTGCTGCGCCGAATTTATGTCAATCTAGAAACGCTCAAGCGCAATGGGGATAAATTTGCTGCCACGATTCAAGAGAAACTGAGGATAATTGTTGCAGGTGGTGATGGAACTGCTGGCTGGCTTCTTGGAGTTGTTTCTGATCTGAAATTAGCTCGTCCGCCACCGATAGCAACAGTACCCTTAGGAACTGGAAACAATCTTCCATTTGCATTTGGCTGGGGGAAGAAGAATCCTGGAACAGATCAACATGCTGTGGAGTCCTTTTTGCGTCAAGTAAAGGGAGCCAAGGAAATGAAGATAGACAGCTGGCACCTTCTCATGAGGATGAAGATTCCAAAAGGAGATCCATTGGATCCCATTGCGCCTCTTGAGCTACCCCATTCTTTGCATGCCTTTGGCCGTGTCTCTGAGACAAACGAGCTGAACATGGAGGGCTTCCATACATTTCGTGGGGGATTTTGGAATTACTTCAGTATGGGAATGGATGCTGAAGTATCATATGCGTTTCATTCAGAGCGGAAGTTGCATCCTGAAAAGTTTAAAAATCAGTTAGTTAATCAGTCTACTTATGCAAAGCTTGGGTGTACACAAGGCTGGTTTTCTCCTTCCCTTTCTCAGAATTCTGCCCGGAATATAGCTCAATTTGCAAAGGTGATGATTATAAAAAACCATGGGGATCAATGGGAAGAGCTTTTCCTTCCTCACAGCATCAAGTCGATAGTCTGCCTCAATTTGCCTAGCTTTTCTGGTGGATTTAATCCTTGGGGAACACCACGTAGACGAAAGCATAATGCATTCACCCCACCATATGTAGATGATGGCCATCTAGAGGTTGTAGGTTTTACTTCTGCTTGGCATGGACTTGTATTGCTTGCGCCAAATGGACATGGGACTCGTCTTGCTCAGGCCCACCGAATCAAATTTCTGTTTCACAAAGGTGCAATTGATCAGACGTACATGAGAATTGATGGGGAACCCTGGAGGCAACCCCTTCCATCTGATGATGACACTGTGATGGTGGAAATCTCTCACCTTGGCCAGGTCAACATTCTCGCTACCAATGGCTGCAGGTCTAAAAGTATGCACAATCCCTCAACTCCTACAGCTCATGAAGAGGAAGGAGTTGACTCAGATGAAGAAAATCTTGAAGCGGAGTTCAGGAAGTTTGGTGCAGCAGACACATTCAAGCTTCCGGACGAAATTGATATTTCTCATCTTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001775 GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005886 GO:0006629 GO:0006793 GO:0006796 GO:0006950 GO:0007596 GO:0007599 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0030168 GO:0030258 GO:0030425 GO:0031974 GO:0031981 GO:0032501 GO:0032991 GO:0036477 GO:0042060 GO:0042995 GO:0043005 GO:0043197 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044255 GO:0044309 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044456 GO:0044463 GO:0044464 GO:0045202 GO:0046834 GO:0050817 GO:0050878 GO:0050896 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097447 GO:0097458 GO:0098794 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

497

Amino Acids

55.5

Weight (kDa)

6.32

Isoelectric Point (pI)

42.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	49 - 173	1.3e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	242 - 415	3.7e-38	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	929
AarI	CACCTGC	1 cut(s)	353
Acc36I	ACCTGC	3 cut(s)	223, 353, 1326
AccB1I	GGYRCC	1 cut(s)	576
AccBSI	CCGCTC	1 cut(s)	784
AccI	GTMKAC	2 cut(s)	830, 1045
AccIII	TCCGGA	2 cut(s)	259, 1459
AciI	CCGC	3 cut(s)	425, 784, 1418
AclWI	GGATC	4 cut(s)	605, 614, 627, 951
AcoI	YGGCCR	4 cut(s)	107, 664, 1087, 1303
AcsI	RAATTY	4 cut(s)	279, 320, 886, 1187
AcuI	CTGAAG	2 cut(s)	721, 777
AfaI	GTAC	3 cut(s)	444, 853, 1223
AfiI	CCNNNNNNNGG	2 cut(s)	1096, 1306
AgsI	TTSAA	3 cut(s)	338, 1415, 1453
AhdI	GACNNNNNGTC	1 cut(s)	210
AjnI	CCWGG	3 cut(s)	499, 1244, 1305
AleI	CACNNNNGTG	1 cut(s)	1203
Alw26I	GTCTC	2 cut(s)	671, 676
AlwI	GGATC	4 cut(s)	605, 614, 627, 951
Ama87I	CYCGRG	1 cut(s)	137
Aor13HI	TCCGGA	2 cut(s)	259, 1459
AoxI	GGCC	5 cut(s)	107, 664, 1087, 1173, 1303
ApeKI	GCWGC	5 cut(s)	51, 271, 326, 1332, 1439
ApoI	RAATTY	4 cut(s)	279, 320, 886, 1187
Asp700I	GAANNNNTTC	1 cut(s)	734
AspLEI	GCGC	4 cut(s)	276, 309, 631, 1144
AspS9I	GGNCC	1 cut(s)	1174
AsuC2I	CCSGG	1 cut(s)	895
AsuHPI	GGTGA	4 cut(s)	380, 931, 1057, 1289
AvaI	CYCGRG	1 cut(s)	137
AxyI	CCTNAGG	1 cut(s)	448
BalI	TGGCCA	3 cut(s)	109, 1089, 1305
BamHI	GGATCC	1 cut(s)	619
BanI	GGYRCC	1 cut(s)	576
BanII	GRGCYC	1 cut(s)	138
BbsI	GAAGAC	1 cut(s)	24
BbvI	GCAGC	5 cut(s)	38, 258, 313, 1319, 1451
BccI	CCATC	7 cut(s)	99, 365, 1079, 1098, 1229, 1270, 1276
BceAI	ACGGC	1 cut(s)	651
BciT130I	CCWGG	3 cut(s)	501, 1246, 1307
BclI	TGATCA	1 cut(s)	1213
BcnI	CCSGG	1 cut(s)	895
BcoDI	GTCTC	2 cut(s)	671, 676
BfaI	CTAG	3 cut(s)	293, 1005, 1094
BfmI	CTRYAG	3 cut(s)	71, 1333, 1368
BfuAI	ACCTGC	3 cut(s)	223, 353, 1326
BisI	GCNGC	5 cut(s)	52, 272, 327, 1333, 1440
BlsI	GCNGC	5 cut(s)	53, 273, 328, 1334, 1441
Bme1390I	CCNGG	4 cut(s)	501, 895, 1246, 1307
BmeRI	GACNNNNNGTC	1 cut(s)	210
BmeT110I	CYCGRG	1 cut(s)	137
BmgT120I	GGNCC	1 cut(s)	1174
BmiI	GGNNCC	5 cut(s)	258, 550, 578, 621, 1242
BmrFI	CCNGG	4 cut(s)	501, 895, 1246, 1307
BmsI	GCATC	3 cut(s)	742, 802, 984
BpiI	GAAGAC	1 cut(s)	24
BpmI	CTGGAG	1 cut(s)	1267
Bpu10I	CCTNAGC	1 cut(s)	1170
BpuEI	CTTGAG	2 cut(s)	287, 656
BpuMI	CCSGG	1 cut(s)	895
BsaAI	YACGTR	1 cut(s)	1043
BsaBI	GATNNNNATC	1 cut(s)	1089
BsaJI	CCNNGG	5 cut(s)	552, 937, 1028, 1244, 1300
BsaWI	WCCGGW	2 cut(s)	259, 1459
Bsc4I	CCNNNNNNNGG	2 cut(s)	1096, 1306
Bse1I	ACTGG	3 cut(s)	146, 233, 460
Bse21I	CCTNAGG	1 cut(s)	448
Bse3DI	GCAATG	2 cut(s)	316, 624
Bse8I	GATNNNNATC	1 cut(s)	1089
BseAI	TCCGGA	2 cut(s)	259, 1459
BseBI	CCWGG	3 cut(s)	501, 1246, 1307
BseDI	CCNNGG	5 cut(s)	552, 937, 1028, 1244, 1300
BseGI	GGATG	3 cut(s)	597, 757, 793
BseJI	GATNNNNATC	1 cut(s)	1089
BseLI	CCNNNNNNNGG	2 cut(s)	1096, 1306
BseMI	GCAATG	2 cut(s)	316, 624
BseMII	CTCAG	7 cut(s)	69, 92, 338, 666, 896, 1184, 1410
BseNI	ACTGG	3 cut(s)	146, 233, 460
BseRI	GAGGAG	1 cut(s)	209
BseXI	GCAGC	5 cut(s)	38, 258, 313, 1319, 1451
BseYI	CCCAGC	1 cut(s)	483
BsgI	GTGCAG	1 cut(s)	1458
BshFI	GGCC	5 cut(s)	109, 666, 1089, 1175, 1305
BshNI	GGYRCC	1 cut(s)	576
BsiHKCI	CYCGRG	1 cut(s)	137
BsiSI	CCGG	3 cut(s)	260, 895, 1460
BslFI	GGGAC	1 cut(s)	1171
BslI	CCNNNNNNNGG	2 cut(s)	1096, 1306
BsmAI	GTCTC	2 cut(s)	671, 676
BsmFI	GGGAC	1 cut(s)	1171
BsmI	GAATGC	1 cut(s)	1061
BsnI	GGCC	5 cut(s)	109, 666, 1089, 1175, 1305
BsoBI	CYCGRG	1 cut(s)	137
Bsp1286I	GDGCHC	1 cut(s)	138
Bsp13I	TCCGGA	2 cut(s)	259, 1459
Bsp1407I	TGTACA	1 cut(s)	851
Bsp143I	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
Bsp19I	CCATGG	1 cut(s)	937
BspACI	CCGC	3 cut(s)	425, 784, 1418
BspANI	GGCC	5 cut(s)	109, 666, 1089, 1175, 1305
BspCNI	CTCAG	7 cut(s)	68, 93, 339, 667, 895, 1183, 1409
BspEI	TCCGGA	2 cut(s)	259, 1459
BspHI	TCATGA	2 cut(s)	585, 1375
BspLI	GGNNCC	5 cut(s)	258, 550, 578, 621, 1242
BspMAI	CTGCAG	1 cut(s)	1337
BspMI	ACCTGC	3 cut(s)	223, 353, 1326
BspPI	GGATC	4 cut(s)	605, 614, 627, 951
BspQI	GCTCTTC	1 cut(s)	948
BspT107I	GGYRCC	1 cut(s)	576
BsrBI	CCGCTC	1 cut(s)	784
BsrDI	GCAATG	2 cut(s)	316, 624
BsrGI	TGTACA	1 cut(s)	851
BsrI	ACTGG	3 cut(s)	146, 233, 460
BssECI	CCNNGG	5 cut(s)	552, 937, 1028, 1244, 1300
BssMI	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
BssT1I	CCWWGG	4 cut(s)	552, 937, 1028, 1300
Bst2UI	CCWGG	3 cut(s)	501, 1246, 1307
Bst4CI	ACNGT	2 cut(s)	442, 1279
Bst6I	CTCTTC	4 cut(s)	31, 246, 948, 1374
BstAUI	TGTACA	1 cut(s)	851
BstBAI	YACGTR	1 cut(s)	1043
BstC8I	GCNNGC	5 cut(s)	382, 386, 575, 657, 1140
BstDEI	CTNAG	9 cut(s)	55, 101, 347, 448, 675, 882, 1170, 1396, 1484
BstDSI	CCRYGG	1 cut(s)	937
BstENI	CCTNNNNNAGG	1 cut(s)	1304
BstF5I	GGATG	3 cut(s)	597, 757, 793
BstHHI	GCGC	4 cut(s)	276, 309, 631, 1144
BstKTI	GATC	6 cut(s)	409, 511, 613, 622, 946, 1216
BstMAI	GTCTC	2 cut(s)	671, 676
BstMBI	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
BstMWI	GCNNNNNNNGC	3 cut(s)	637, 790, 911
BstNI	CCWGG	3 cut(s)	501, 1246, 1307
BstNSI	RCATGY	2 cut(s)	518, 659
BstSCI	CCNGG	4 cut(s)	499, 893, 1244, 1305
BstSFI	CTRYAG	3 cut(s)	71, 1333, 1368
BstV1I	GCAGC	5 cut(s)	38, 258, 313, 1319, 1451
BstV2I	GAAGAC	1 cut(s)	24
BstX2I	RGATCY	2 cut(s)	610, 619
BstYI	RGATCY	2 cut(s)	610, 619
Bsu36I	CCTNAGG	1 cut(s)	448
BsuRI	GGCC	5 cut(s)	109, 666, 1089, 1175, 1305
BtgI	CCRYGG	1 cut(s)	937
BtsCI	GGATG	3 cut(s)	597, 757, 793
BtsIMutI	CAGTG	1 cut(s)	1275
BveI	ACCTGC	3 cut(s)	223, 353, 1326
Cac8I	GCNNGC	5 cut(s)	382, 386, 575, 657, 1140
CciI	TCATGA	2 cut(s)	585, 1375
CfoI	GCGC	4 cut(s)	276, 309, 631, 1144
Cfr13I	GGNCC	1 cut(s)	1174
CseI	GACGC	1 cut(s)	524
Csp6I	GTAC	3 cut(s)	443, 852, 1222
CspCI	CAANNNNNGTGG	2 cut(s)	157, 192
CviAII	CATG	9 cut(s)	515, 586, 656, 694, 938, 1124, 1154, 1225, 1376
CviQI	GTAC	3 cut(s)	443, 852, 1222
DdeI	CTNAG	9 cut(s)	55, 101, 347, 448, 675, 882, 1170, 1396, 1484
DpnI	GATC	6 cut(s)	408, 510, 612, 621, 945, 1215
DpnII	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
DraI	TTTAAA	1 cut(s)	808
DriI	GACNNNNNGTC	1 cut(s)	210
EaeI	YGGCCR	4 cut(s)	107, 664, 1087, 1303
Eam1104I	CTCTTC	4 cut(s)	31, 246, 948, 1374
Eam1105I	GACNNNNNGTC	1 cut(s)	210
EarI	CTCTTC	4 cut(s)	31, 246, 948, 1374
EciI	GGCGGA	1 cut(s)	414
Eco130I	CCWWGG	4 cut(s)	552, 937, 1028, 1300
Eco24I	GRGCYC	1 cut(s)	138
Eco57I	CTGAAG	2 cut(s)	721, 777
Eco81I	CCTNAGG	1 cut(s)	448
Eco88I	CYCGRG	1 cut(s)	137
EcoNI	CCTNNNNNAGG	1 cut(s)	1304
EcoRI	GAATTC	1 cut(s)	886
EcoRII	CCWGG	3 cut(s)	499, 1244, 1305
EcoT14I	CCWWGG	4 cut(s)	552, 937, 1028, 1300
EcoT22I	ATGCAT	1 cut(s)	1063
EcoT38I	GRGCYC	1 cut(s)	138
ErhI	CCWWGG	4 cut(s)	552, 937, 1028, 1300
FaeI	CATG	9 cut(s)	518, 589, 659, 697, 941, 1127, 1157, 1228, 1379
FalI	AAGNNNNNCTT	2 cut(s)	1395, 1427
FaqI	GGGAC	1 cut(s)	1171
FatI	CATG	9 cut(s)	514, 585, 655, 693, 937, 1123, 1153, 1224, 1375
FauNDI	CATATG	2 cut(s)	766, 1075
FbaI	TGATCA	1 cut(s)	1213
FblI	GTMKAC	2 cut(s)	830, 1045
Fnu4HI	GCNGC	5 cut(s)	52, 272, 327, 1333, 1440
FokI	GGATG	3 cut(s)	604, 764, 780
FriOI	GRGCYC	1 cut(s)	138
Fsp4HI	GCNGC	5 cut(s)	52, 272, 327, 1333, 1440
FspBI	CTAG	3 cut(s)	293, 1005, 1094
GlaI	GCGC	4 cut(s)	275, 308, 630, 1143
GluI	GCNGC	5 cut(s)	52, 272, 327, 1333, 1440
GsaI	CCCAGC	1 cut(s)	487
GsuI	CTGGAG	1 cut(s)	1267
HaeIII	GGCC	5 cut(s)	109, 666, 1089, 1175, 1305
HapII	CCGG	3 cut(s)	260, 895, 1460
HgaI	GACGC	1 cut(s)	524
HhaI	GCGC	4 cut(s)	276, 309, 631, 1144
Hin1II	CATG	9 cut(s)	518, 589, 659, 697, 941, 1127, 1157, 1228, 1379
Hin6I	GCGC	4 cut(s)	274, 307, 629, 1142
HinP1I	GCGC	4 cut(s)	274, 307, 629, 1142
HincII	GTYRAC	2 cut(s)	1312, 1393
HindII	GTYRAC	2 cut(s)	1312, 1393
HindIII	AAGCTT	2 cut(s)	842, 1454
HinfI	GANTC	9 cut(s)	38, 125, 334, 496, 524, 598, 1159, 1182, 1394
HpaII	CCGG	3 cut(s)	260, 895, 1460
HphI	GGTGA	4 cut(s)	380, 931, 1057, 1289
Hpy166II	GTNNAC	6 cut(s)	831, 852, 854, 1046, 1312, 1393
Hpy8I	GTNNAC	6 cut(s)	831, 852, 854, 1046, 1312, 1393
HpyAV	CCTTC	5 cut(s)	590, 882, 974, 1268, 1379
HpyCH4III	ACNGT	2 cut(s)	442, 1279
HpyCH4IV	ACGT	3 cut(s)	206, 1042, 1220
HpyF10VI	GCNNNNNNNGC	3 cut(s)	637, 790, 911
HpyF3I	CTNAG	9 cut(s)	55, 101, 347, 448, 675, 882, 1170, 1396, 1484
HpySE526I	ACGT	3 cut(s)	206, 1042, 1220
Hsp92II	CATG	9 cut(s)	518, 589, 659, 697, 941, 1127, 1157, 1228, 1379
HspAI	GCGC	4 cut(s)	274, 307, 629, 1142
Kpn2I	TCCGGA	2 cut(s)	259, 1459
Ksp22I	TGATCA	1 cut(s)	1213
Kzo9I	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
LguI	GCTCTTC	1 cut(s)	948
LmnI	GCTCC	4 cut(s)	89, 222, 262, 548
Lsp1109I	GCAGC	5 cut(s)	38, 258, 313, 1319, 1451
LweI	GCATC	3 cut(s)	742, 802, 984
MaeI	CTAG	3 cut(s)	293, 1005, 1094
MaeII	ACGT	3 cut(s)	206, 1042, 1220
MaeIII	GTNAC	1 cut(s)	202
MalI	GATC	6 cut(s)	408, 510, 612, 621, 945, 1215
MbiI	CCGCTC	1 cut(s)	784
MboI	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
MfeI	CAATTG	1 cut(s)	1209
MflI	RGATCY	2 cut(s)	610, 619
MhlI	GDGCHC	1 cut(s)	138
MlsI	TGGCCA	3 cut(s)	109, 1089, 1305
MluNI	TGGCCA	3 cut(s)	109, 1089, 1305
MlyI	GAGTC	3 cut(s)	533, 1153, 1388
Mox20I	TGGCCA	3 cut(s)	109, 1089, 1305
Mph1103I	ATGCAT	1 cut(s)	1063
MroI	TCCGGA	2 cut(s)	259, 1459
MroXI	GAANNNNTTC	1 cut(s)	734
MscI	TGGCCA	3 cut(s)	109, 1089, 1305
MseI	TTAA	5 cut(s)	65, 807, 822, 1023, 1489
MslI	CAYNNNNRTG	3 cut(s)	590, 1203, 1280
Msp20I	TGGCCA	3 cut(s)	109, 1089, 1305
MspA1I	CMGCKG	1 cut(s)	573
MspI	CCGG	3 cut(s)	260, 895, 1460
MspR9I	CCNGG	4 cut(s)	501, 895, 1246, 1307
MunI	CAATTG	1 cut(s)	1209
Mva1269I	GAATGC	1 cut(s)	1061
MvaI	CCWGG	3 cut(s)	501, 1246, 1307
MwoI	GCNNNNNNNGC	3 cut(s)	637, 790, 911
NciI	CCSGG	1 cut(s)	895
NcoI	CCATGG	1 cut(s)	937
NdeI	CATATG	2 cut(s)	766, 1075
NdeII	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
NlaIII	CATG	9 cut(s)	518, 589, 659, 697, 941, 1127, 1157, 1228, 1379
NlaIV	GGNNCC	5 cut(s)	258, 550, 578, 621, 1242
NmuCI	GTSAC	1 cut(s)	202
NsiI	ATGCAT	1 cut(s)	1063
NspI	RCATGY	2 cut(s)	518, 659
OliI	CACNNNNGTG	1 cut(s)	1203
PaeI	GCATGC	1 cut(s)	659
PagI	TCATGA	2 cut(s)	585, 1375
PaqCI	CACCTGC	1 cut(s)	353
PciSI	GCTCTTC	1 cut(s)	948
PctI	GAATGC	1 cut(s)	1061
PdmI	GAANNNNTTC	1 cut(s)	734
PfeI	GAWTC	6 cut(s)	38, 125, 334, 496, 598, 1182
PfoI	TCCNGGA	1 cut(s)	499
PkrI	GCNGC	5 cut(s)	53, 273, 328, 1334, 1441
PleI	GAGTC	3 cut(s)	532, 1153, 1388
PpsI	GAGTC	3 cut(s)	532, 1153, 1388
Ppu21I	YACGTR	1 cut(s)	1043
PsiI	TTATAA	1 cut(s)	929
Psp6I	CCWGG	3 cut(s)	499, 1244, 1305
PspFI	CCCAGC	1 cut(s)	483
PspGI	CCWGG	3 cut(s)	499, 1244, 1305
PspN4I	GGNNCC	5 cut(s)	258, 550, 578, 621, 1242
PspPI	GGNCC	1 cut(s)	1174
PstI	CTGCAG	1 cut(s)	1337
PsuI	RGATCY	2 cut(s)	610, 619
PvuII	CAGCTG	1 cut(s)	573
RsaI	GTAC	3 cut(s)	444, 853, 1223
RsaNI	GTAC	3 cut(s)	443, 852, 1222
RseI	CAYNNNNRTG	3 cut(s)	590, 1203, 1280
SapI	GCTCTTC	1 cut(s)	948
SaqAI	TTAA	5 cut(s)	65, 807, 822, 1023, 1489
SatI	GCNGC	5 cut(s)	52, 272, 327, 1333, 1440
Sau3AI	GATC	6 cut(s)	406, 508, 610, 619, 943, 1213
Sau96I	GGNCC	1 cut(s)	1174
SchI	GAGTC	3 cut(s)	533, 1153, 1388
ScrFI	CCNGG	4 cut(s)	501, 895, 1246, 1307
SduI	GDGCHC	1 cut(s)	138
SfaNI	GCATC	3 cut(s)	742, 802, 984
SfcI	CTRYAG	3 cut(s)	71, 1333, 1368
SmiMI	CAYNNNNRTG	3 cut(s)	590, 1203, 1280
SmlI	CTYRAG	2 cut(s)	302, 635
SmoI	CTYRAG	2 cut(s)	302, 635
SphI	GCATGC	1 cut(s)	659
SsiI	CCGC	3 cut(s)	425, 784, 1418
SspMI	CTAG	3 cut(s)	293, 1005, 1094
StyD4I	CCNGG	4 cut(s)	499, 893, 1244, 1305
StyI	CCWWGG	4 cut(s)	552, 937, 1028, 1300
TaaI	ACNGT	2 cut(s)	442, 1279
TaiI	ACGT	3 cut(s)	209, 1045, 1223
TaqI	TCGA	1 cut(s)	983
TatI	WGTACW	1 cut(s)	851
TfiI	GAWTC	6 cut(s)	38, 125, 334, 496, 598, 1182
Tru1I	TTAA	5 cut(s)	65, 807, 822, 1023, 1489
Tru9I	TTAA	5 cut(s)	65, 807, 822, 1023, 1489
TscAI	CASTG	1 cut(s)	1282
TseFI	GTSAC	1 cut(s)	202
TseI	GCWGC	5 cut(s)	51, 271, 326, 1332, 1439
Tsp45I	GTSAC	1 cut(s)	202
TspDTI	ATGAA	7 cut(s)	17, 21, 575, 608, 764, 1392, 1416
TspRI	CASTG	1 cut(s)	1282
XagI	CCTNNNNNAGG	1 cut(s)	1304
XapI	RAATTY	4 cut(s)	279, 320, 886, 1187
XbaI	TCTAGA	2 cut(s)	292, 1093
XceI	RCATGY	2 cut(s)	518, 659
XcmI	CCANNNNNNNNNTGG	1 cut(s)	713
XmiI	GTMKAC	2 cut(s)	830, 1045
XmnI	GAANNNNTTC	1 cut(s)	734
XspI	CTAG	3 cut(s)	293, 1005, 1094
Zsp2I	ATGCAT	1 cut(s)	1063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.