Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

7079004 .. 7084150

Gene Structure 5147 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1097300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGTTCATGGTTAGAGGCTCACATCTCCTCAACTCACTATTTAGACCCAAAATGGCAAATTCTAACTATGGGGAGGAATTCTTGAAGGATTTCAGAATTCCCAAGTATGTACTAGTGCCTGAATCAAAGAGTGAAAATGTTGCTGATGTACCGCAATGTCCGGTTTTAGTGTTTATCAACTCCAAAAGTGGTGGCCAGCTTGGTGGGGATCTTCTTGTATCATACCGTTCCCTTCTAAATGAAAGTCAGGTTTATGATTTGGGGCAAGAGGCTCCGGATAAGGTTCTTTGCCGAATCTATGCCAATCTTGAAAAACTCAAGCGCAATGGGGATAAATTCGCTACAAAAATTCAAGAGAAATTGAGAATCATTGTTGCAGGTGGCGATGGAACAGCTGGTTGGCTTCTTGGAGTTGTTTCTGATCTCAAGTTATCTCATTCGCCACCAATAGCAACAGTACCACTAGGAACAGGAAACAATCTTCCTTTTGCATTTGGATGGGGGAAGAAGAATCCTGGGACAGATCCTCAATCTGTGAAGCAGTTTTTGGACCAAGTAAAGAGAGCCAAGGAAATGAAAATAGACAGCTGGCACATTCTCATGAGGATGAGGAAGCCAAAAGAAGGTCCGTGTGATCCCATTGCGCCTCTTGACCTACCTCATTCGTTGCATGCATTTGGCCGTGTCTCTGATTCAAATGAGCTGAACATGGAAGGCCGCCATACCTTTCGTGGGGGATTTTGGAACTACTTCAGTATGGGAATGGATGCTCAAGTATCATATGCATTTCATACTGAGCGAAAGCAGCACCCCGAAAAGTTCAAAAATCAGTTAACTAATCAGACTACTTATGCAAAGATTACCCATTCACAAGGCTGGTTTTCTTCTCCTTGTTCTCAATCTACTTCACGGGACATAGCGCAATTTGCAAAGGTGAAGGTCATGAAAAACCATGGCCAATGGGAAGATCTACAAATTCCTCCAAGTATCAAGTCAATCGTGATCCTCAATTTGCCTAGCTTTTCTGGTGGTTTTAATCCTTGGGGAGTCCCGATTAGGAAGAGGCGTAATGGAGGTTTTACTCCAGCATATGTAGACGATGGTCTCATTGAAGTTGTTGGCTTTCGAGATGCATGGCATGGGCTTGTTTTGCTTGCCAAGGGACACGGGACTCGTCTTGCCCAGGCCCACCGTATTCGCTTTGAGTTTCAAAAGGGTGCAATTGATCATACGTACATGAGGATCGATGGGGAACCCTGGAAGCAACCCCTCCCAGTTGACGATGAAACTGTCGTGGTTGAAATCTCTCACCTTCGCCAGGTGAACATTCTTGCTCGACATGGCTGCAGGTCTAAAAGCGTTTATAATCCCGAGTCACCTAGCAGCCAGGGAAATGAGGAAGAGGATTGGGATGATGAAGAAACTCATGAATCAGCATTGTATAGGAAGTTTGGTGCAGCAGACACATTTAAGCTCCCCGAAGAGATTGATATTTCGCATCTCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001775 GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005886 GO:0006629 GO:0006793 GO:0006796 GO:0006950 GO:0007596 GO:0007599 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0030168 GO:0030258 GO:0030425 GO:0031974 GO:0031981 GO:0032501 GO:0032991 GO:0036477 GO:0042060 GO:0042995 GO:0043005 GO:0043197 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044255 GO:0044309 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044456 GO:0044463 GO:0044464 GO:0045202 GO:0046834 GO:0050817 GO:0050878 GO:0050896 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097447 GO:0097458 GO:0098794 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

503

Amino Acids

56.52

Weight (kDa)

8.57

Isoelectric Point (pI)

43.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	54 - 179	3.8e-20	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	247 - 419	5.2e-38	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1365
AarI	CACCTGC	1 cut(s)	368
Acc36I	ACCTGC	2 cut(s)	368, 1338
AccI	GTMKAC	1 cut(s)	1095
AccIII	TCCGGA	1 cut(s)	274
AciI	CCGC	2 cut(s)	152, 718
AclWI	GGATC	5 cut(s)	216, 518, 629, 997, 1250
AcoI	YGGCCR	3 cut(s)	193, 679, 955
AcsI	RAATTY	6 cut(s)	58, 77, 96, 335, 348, 975
AcuI	CTGAAG	1 cut(s)	736
AfaI	GTAC	4 cut(s)	111, 150, 459, 1235
AfiI	CCNNNNNNNGG	3 cut(s)	623, 732, 1318
AgsI	TTSAA	8 cut(s)	85, 311, 353, 696, 823, 1112, 1211, 1301
AhlI	ACTAGT	1 cut(s)	112
AjnI	CCWGG	5 cut(s)	514, 1182, 1256, 1317, 1386
AjuI	GAANNNNNNNTTGG	4 cut(s)	382, 414, 530, 562
AluBI	AGCT	6 cut(s)	199, 395, 588, 703, 1020, 1474
AluI	AGCT	6 cut(s)	199, 395, 588, 703, 1020, 1474
Alw26I	GTCTC	2 cut(s)	691, 1109
AlwI	GGATC	5 cut(s)	216, 518, 629, 997, 1250
Ama87I	CYCGRG	1 cut(s)	1370
Aor13HI	TCCGGA	1 cut(s)	274
AoxI	GGCC	5 cut(s)	193, 679, 715, 955, 1185
ApeKI	GCWGC	4 cut(s)	805, 1344, 1383, 1457
ApoI	RAATTY	6 cut(s)	58, 77, 96, 335, 348, 975
Asp700I	GAANNNNTTC	2 cut(s)	89, 749
AspLEI	GCGC	3 cut(s)	324, 646, 922
AspS9I	GGNCC	3 cut(s)	550, 626, 1186
AsuHPI	GGTGA	4 cut(s)	946, 1301, 1333, 1368
AvaI	CYCGRG	1 cut(s)	1370
AvaII	GGWCC	2 cut(s)	550, 626
BalI	TGGCCA	2 cut(s)	195, 957
BbvI	GCAGC	4 cut(s)	817, 1331, 1395, 1469
BccI	CCATC	4 cut(s)	380, 492, 1094, 1241
BceAI	ACGGC	1 cut(s)	666
BcgI	CGANNNNNNTGC	2 cut(s)	1326, 1360
BciT130I	CCWGG	5 cut(s)	516, 1184, 1258, 1319, 1388
BclI	TGATCA	1 cut(s)	1225
BcoDI	GTCTC	2 cut(s)	691, 1109
BcuI	ACTAGT	1 cut(s)	112
BfaI	CTAG	4 cut(s)	113, 464, 1017, 1380
BfmI	CTRYAG	1 cut(s)	1345
BfuAI	ACCTGC	2 cut(s)	368, 1338
BglII	AGATCT	1 cut(s)	967
BisI	GCNGC	5 cut(s)	718, 806, 1345, 1384, 1458
BlsI	GCNGC	5 cut(s)	719, 807, 1346, 1385, 1459
Bme1390I	CCNGG	5 cut(s)	516, 1184, 1258, 1319, 1388
Bme18I	GGWCC	2 cut(s)	550, 626
BmeT110I	CYCGRG	1 cut(s)	1370
BmgT120I	GGNCC	3 cut(s)	550, 626, 1186
BmiI	GGNNCC	2 cut(s)	273, 1254
BmrFI	CCNGG	5 cut(s)	516, 1184, 1258, 1319, 1388
BmrI	ACTGGG	1 cut(s)	1268
BmsI	GCATC	2 cut(s)	757, 1120
BmuI	ACTGGG	1 cut(s)	1268
BoxI	GACNNNNGTC	1 cut(s)	1101
BpmI	CTGGAG	1 cut(s)	1068
BpuEI	CTTGAG	3 cut(s)	302, 410, 756
Bsa29I	ATCGAT	1 cut(s)	1245
BsaAI	YACGTR	1 cut(s)	1233
BsaI	GGTCTC	1 cut(s)	1109
BsaJI	CCNNGG	8 cut(s)	515, 567, 952, 1040, 1158, 1182, 1256, 1387
BsaWI	WCCGGW	2 cut(s)	160, 274
Bsc4I	CCNNNNNNNGG	3 cut(s)	623, 732, 1318
Bse1I	ACTGG	1 cut(s)	1274
Bse3DI	GCAATG	3 cut(s)	161, 331, 639
BseAI	TCCGGA	1 cut(s)	274
BseBI	CCWGG	5 cut(s)	516, 1184, 1258, 1319, 1388
BseCI	ATCGAT	1 cut(s)	1245
BseDI	CCNNGG	8 cut(s)	515, 567, 952, 1040, 1158, 1182, 1256, 1387
BseGI	GGATG	4 cut(s)	503, 612, 772, 1417
BseLI	CCNNNNNNNGG	3 cut(s)	623, 732, 1318
BseMI	GCAATG	3 cut(s)	161, 331, 639
BseMII	CTCAG	1 cut(s)	786
BseNI	ACTGG	1 cut(s)	1274
BseRI	GAGGAG	1 cut(s)	17
BseXI	GCAGC	4 cut(s)	817, 1331, 1395, 1469
BsgI	GTGCAG	1 cut(s)	1476
BshFI	GGCC	5 cut(s)	195, 681, 717, 957, 1187
BshVI	ATCGAT	1 cut(s)	1245
BsiHKCI	CYCGRG	1 cut(s)	1370
BsiSI	CCGG	2 cut(s)	161, 275
BslFI	GGGAC	5 cut(s)	532, 926, 1034, 1176, 1183
BslI	CCNNNNNNNGG	3 cut(s)	623, 732, 1318
BsmAI	GTCTC	2 cut(s)	691, 1109
BsmFI	GGGAC	5 cut(s)	532, 926, 1034, 1176, 1183
BsnI	GGCC	5 cut(s)	195, 681, 717, 957, 1187
Bso31I	GGTCTC	1 cut(s)	1109
BsoBI	CYCGRG	1 cut(s)	1370
Bsp13I	TCCGGA	1 cut(s)	274
Bsp143I	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
Bsp19I	CCATGG	1 cut(s)	952
BspACI	CCGC	2 cut(s)	152, 718
BspANI	GGCC	5 cut(s)	195, 681, 717, 957, 1187
BspCNI	CTCAG	1 cut(s)	787
BspDI	ATCGAT	1 cut(s)	1245
BspEI	TCCGGA	1 cut(s)	274
BspHI	TCATGA	3 cut(s)	600, 942, 1426
BspLI	GGNNCC	2 cut(s)	273, 1254
BspMAI	CTGCAG	1 cut(s)	1349
BspMI	ACCTGC	2 cut(s)	368, 1338
BspPI	GGATC	5 cut(s)	216, 518, 629, 997, 1250
BspTNI	GGTCTC	1 cut(s)	1109
BsrDI	GCAATG	3 cut(s)	161, 331, 639
BsrI	ACTGG	1 cut(s)	1274
BssECI	CCNNGG	8 cut(s)	515, 567, 952, 1040, 1158, 1182, 1256, 1387
BssMI	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
BssT1I	CCWWGG	4 cut(s)	567, 952, 1040, 1158
Bst2UI	CCWGG	5 cut(s)	516, 1184, 1258, 1319, 1388
Bst4CI	ACNGT	4 cut(s)	227, 457, 1193, 1291
Bst6I	CTCTTC	3 cut(s)	1055, 1395, 1476
BstBAI	YACGTR	1 cut(s)	1233
BstC8I	GCNNGC	4 cut(s)	197, 590, 672, 1155
BstDEI	CTNAG	2 cut(s)	795, 1502
BstDSI	CCRYGG	1 cut(s)	952
BstENI	CCTNNNNNAGG	1 cut(s)	1316
BstF5I	GGATG	4 cut(s)	503, 612, 772, 1417
BstHHI	GCGC	3 cut(s)	324, 646, 922
BstKTI	GATC	8 cut(s)	211, 424, 526, 637, 970, 1005, 1228, 1245
BstMAI	GTCTC	2 cut(s)	691, 1109
BstMBI	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
BstMWI	GCNNNNNNNGC	3 cut(s)	805, 926, 1150
BstNI	CCWGG	5 cut(s)	516, 1184, 1258, 1319, 1388
BstNSI	RCATGY	1 cut(s)	674
BstPAI	GACNNNNGTC	1 cut(s)	1101
BstSCI	CCNGG	5 cut(s)	514, 1182, 1256, 1317, 1386
BstSFI	CTRYAG	1 cut(s)	1345
BstSNI	TACGTA	1 cut(s)	1233
BstV1I	GCAGC	4 cut(s)	817, 1331, 1395, 1469
BstX2I	RGATCY	3 cut(s)	208, 523, 967
BstXI	CCANNNNNNTGG	1 cut(s)	203
BstYI	RGATCY	3 cut(s)	208, 523, 967
Bsu15I	ATCGAT	1 cut(s)	1245
BsuRI	GGCC	5 cut(s)	195, 681, 717, 957, 1187
BsuTUI	ATCGAT	1 cut(s)	1245
BtgI	CCRYGG	1 cut(s)	952
BtgZI	GCGATG	1 cut(s)	399
BtsCI	GGATG	4 cut(s)	503, 612, 772, 1417
BveI	ACCTGC	2 cut(s)	368, 1338
Cac8I	GCNNGC	4 cut(s)	197, 590, 672, 1155
CciI	TCATGA	3 cut(s)	600, 942, 1426
CfoI	GCGC	3 cut(s)	324, 646, 922
Cfr13I	GGNCC	3 cut(s)	550, 626, 1186
ClaI	ATCGAT	1 cut(s)	1245
Csp6I	GTAC	4 cut(s)	110, 149, 458, 1234
CspCI	CAANNNNNGTGG	2 cut(s)	172, 207
CviQI	GTAC	4 cut(s)	110, 149, 458, 1234
DdeI	CTNAG	2 cut(s)	795, 1502
DpnI	GATC	8 cut(s)	210, 423, 525, 636, 969, 1004, 1227, 1244
DpnII	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
EaeI	YGGCCR	3 cut(s)	193, 679, 955
Eam1104I	CTCTTC	3 cut(s)	1055, 1395, 1476
EarI	CTCTTC	3 cut(s)	1055, 1395, 1476
Eco105I	TACGTA	1 cut(s)	1233
Eco130I	CCWWGG	4 cut(s)	567, 952, 1040, 1158
Eco31I	GGTCTC	1 cut(s)	1109
Eco47I	GGWCC	2 cut(s)	550, 626
Eco57I	CTGAAG	1 cut(s)	736
Eco88I	CYCGRG	1 cut(s)	1370
EcoNI	CCTNNNNNAGG	1 cut(s)	1316
EcoRI	GAATTC	2 cut(s)	77, 96
EcoRII	CCWGG	5 cut(s)	514, 1182, 1256, 1317, 1386
EcoT14I	CCWWGG	4 cut(s)	567, 952, 1040, 1158
EcoT22I	ATGCAT	3 cut(s)	676, 787, 1135
ErhI	CCWWGG	4 cut(s)	567, 952, 1040, 1158
FaqI	GGGAC	5 cut(s)	532, 926, 1034, 1176, 1183
FauNDI	CATATG	2 cut(s)	781, 1090
FbaI	TGATCA	1 cut(s)	1225
FblI	GTMKAC	1 cut(s)	1095
Fnu4HI	GCNGC	5 cut(s)	718, 806, 1345, 1384, 1458
FokI	GGATG	4 cut(s)	510, 619, 779, 1424
Fsp4HI	GCNGC	5 cut(s)	718, 806, 1345, 1384, 1458
FspBI	CTAG	4 cut(s)	113, 464, 1017, 1380
GlaI	GCGC	3 cut(s)	323, 645, 921
GluI	GCNGC	5 cut(s)	718, 806, 1345, 1384, 1458
GsuI	CTGGAG	1 cut(s)	1068
HaeIII	GGCC	5 cut(s)	195, 681, 717, 957, 1187
HapII	CCGG	2 cut(s)	161, 275
HhaI	GCGC	3 cut(s)	324, 646, 922
Hin6I	GCGC	3 cut(s)	322, 644, 920
HinP1I	GCGC	3 cut(s)	322, 644, 920
HincII	GTYRAC	2 cut(s)	834, 1279
HindII	GTYRAC	2 cut(s)	834, 1279
HinfI	GANTC	9 cut(s)	122, 294, 366, 511, 692, 1047, 1171, 1373, 1430
HpaI	GTTAAC	1 cut(s)	834
HpaII	CCGG	2 cut(s)	161, 275
HphI	GGTGA	4 cut(s)	946, 1301, 1333, 1368
Hpy166II	GTNNAC	4 cut(s)	834, 1096, 1279, 1324
Hpy188I	TCNGA	4 cut(s)	95, 421, 691, 843
Hpy8I	GTNNAC	4 cut(s)	834, 1096, 1279, 1324
HpyAV	CCTTC	6 cut(s)	79, 242, 617, 707, 931, 1322
HpyCH4III	ACNGT	4 cut(s)	227, 457, 1193, 1291
HpyCH4IV	ACGT	1 cut(s)	1232
HpyF10VI	GCNNNNNNNGC	3 cut(s)	805, 926, 1150
HpyF3I	CTNAG	2 cut(s)	795, 1502
HpySE526I	ACGT	1 cut(s)	1232
HspAI	GCGC	3 cut(s)	322, 644, 920
Kpn2I	TCCGGA	1 cut(s)	274
Ksp22I	TGATCA	1 cut(s)	1225
KspAI	GTTAAC	1 cut(s)	834
Kzo9I	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
LmnI	GCTCC	2 cut(s)	277, 1479
Lsp1109I	GCAGC	4 cut(s)	817, 1331, 1395, 1469
LweI	GCATC	2 cut(s)	757, 1120
MaeI	CTAG	4 cut(s)	113, 464, 1017, 1380
MaeII	ACGT	1 cut(s)	1232
MaeIII	GTNAC	1 cut(s)	1374
MalI	GATC	8 cut(s)	210, 423, 525, 636, 969, 1004, 1227, 1244
MboI	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
MfeI	CAATTG	1 cut(s)	1221
MflI	RGATCY	3 cut(s)	208, 523, 967
MlsI	TGGCCA	2 cut(s)	195, 957
MluNI	TGGCCA	2 cut(s)	195, 957
MlyI	GAGTC	3 cut(s)	1056, 1165, 1382
Mox20I	TGGCCA	2 cut(s)	195, 957
Mph1103I	ATGCAT	3 cut(s)	676, 787, 1135
MroI	TCCGGA	1 cut(s)	274
MroXI	GAANNNNTTC	2 cut(s)	89, 749
MscI	TGGCCA	2 cut(s)	195, 957
MseI	TTAA	4 cut(s)	833, 1035, 1470, 1507
MslI	CAYNNNNRTG	3 cut(s)	496, 599, 605
Msp20I	TGGCCA	2 cut(s)	195, 957
MspA1I	CMGCKG	2 cut(s)	395, 588
MspI	CCGG	2 cut(s)	161, 275
MspR9I	CCNGG	5 cut(s)	516, 1184, 1258, 1319, 1388
MunI	CAATTG	1 cut(s)	1221
MvaI	CCWGG	5 cut(s)	516, 1184, 1258, 1319, 1388
MwoI	GCNNNNNNNGC	3 cut(s)	805, 926, 1150
NcoI	CCATGG	1 cut(s)	952
NdeI	CATATG	2 cut(s)	781, 1090
NdeII	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
NlaIV	GGNNCC	2 cut(s)	273, 1254
NmuCI	GTSAC	1 cut(s)	1374
NsiI	ATGCAT	3 cut(s)	676, 787, 1135
NspI	RCATGY	1 cut(s)	674
PaeI	GCATGC	1 cut(s)	674
PagI	TCATGA	3 cut(s)	600, 942, 1426
PaqCI	CACCTGC	1 cut(s)	368
PdmI	GAANNNNTTC	2 cut(s)	89, 749
PfeI	GAWTC	6 cut(s)	122, 294, 366, 511, 692, 1430
PkrI	GCNGC	5 cut(s)	719, 807, 1346, 1385, 1459
PleI	GAGTC	3 cut(s)	1055, 1165, 1381
PpsI	GAGTC	3 cut(s)	1055, 1165, 1381
Ppu21I	YACGTR	1 cut(s)	1233
PshAI	GACNNNNGTC	1 cut(s)	1101
PsiI	TTATAA	1 cut(s)	1365
Psp6I	CCWGG	5 cut(s)	514, 1182, 1256, 1317, 1386
PspGI	CCWGG	5 cut(s)	514, 1182, 1256, 1317, 1386
PspN4I	GGNNCC	2 cut(s)	273, 1254
PspPI	GGNCC	3 cut(s)	550, 626, 1186
PstI	CTGCAG	1 cut(s)	1349
PsuI	RGATCY	3 cut(s)	208, 523, 967
PvuII	CAGCTG	2 cut(s)	395, 588
RsaI	GTAC	4 cut(s)	111, 150, 459, 1235
RsaNI	GTAC	4 cut(s)	110, 149, 458, 1234
RseI	CAYNNNNRTG	3 cut(s)	496, 599, 605
SaqAI	TTAA	4 cut(s)	833, 1035, 1470, 1507
SatI	GCNGC	5 cut(s)	718, 806, 1345, 1384, 1458
Sau3AI	GATC	8 cut(s)	208, 421, 523, 634, 967, 1002, 1225, 1242
Sau96I	GGNCC	3 cut(s)	550, 626, 1186
SchI	GAGTC	3 cut(s)	1056, 1165, 1382
ScrFI	CCNGG	5 cut(s)	516, 1184, 1258, 1319, 1388
SfaNI	GCATC	2 cut(s)	757, 1120
SfcI	CTRYAG	1 cut(s)	1345
SinI	GGWCC	2 cut(s)	550, 626
SmiMI	CAYNNNNRTG	3 cut(s)	496, 599, 605
SmlI	CTYRAG	3 cut(s)	317, 425, 771
SmoI	CTYRAG	3 cut(s)	317, 425, 771
SnaBI	TACGTA	1 cut(s)	1233
SpeI	ACTAGT	1 cut(s)	112
SphI	GCATGC	1 cut(s)	674
SsiI	CCGC	2 cut(s)	152, 718
SspMI	CTAG	4 cut(s)	113, 464, 1017, 1380
StyD4I	CCNGG	5 cut(s)	514, 1182, 1256, 1317, 1386
StyI	CCWWGG	4 cut(s)	567, 952, 1040, 1158
TaaI	ACNGT	4 cut(s)	227, 457, 1193, 1291
TaiI	ACGT	1 cut(s)	1235
TaqI	TCGA	3 cut(s)	1126, 1245, 1336
TatI	WGTACW	1 cut(s)	109
TauI	GCSGC	1 cut(s)	720
TfiI	GAWTC	6 cut(s)	122, 294, 366, 511, 692, 1430
Tru1I	TTAA	4 cut(s)	833, 1035, 1470, 1507
Tru9I	TTAA	4 cut(s)	833, 1035, 1470, 1507
TseFI	GTSAC	1 cut(s)	1374
TseI	GCWGC	4 cut(s)	805, 1344, 1383, 1457
Tsp45I	GTSAC	1 cut(s)	1374
TspDTI	ATGAA	7 cut(s)	255, 590, 779, 959, 1299, 1431, 1443
TspGWI	ACGGA	1 cut(s)	618
VpaK11BI	GGWCC	2 cut(s)	550, 626
XagI	CCTNNNNNAGG	1 cut(s)	1316
XapI	RAATTY	6 cut(s)	58, 77, 96, 335, 348, 975
XceI	RCATGY	1 cut(s)	674
XcmI	CCANNNNNNNNNTGG	1 cut(s)	728
XmiI	GTMKAC	1 cut(s)	1095
XmnI	GAANNNNTTC	2 cut(s)	89, 749
XspI	CTAG	4 cut(s)	113, 464, 1017, 1380
Zsp2I	ATGCAT	3 cut(s)	676, 787, 1135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.