Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2C

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Forward (+)

71648917 .. 71653487

Gene Structure 4571 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2CG539200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGGCCAACTCCAGCTCTCGGTCTGATTTCTTGAAGAGTTTCAAGATTCCCAAGTATGTACTTGAATCCGAATCAAAGCGCGATGGGGAAGATTGTGATGAGCCCGAGTGTCCAGTTTTGGTGTTTATCAACTCCAAAAGTGGTGGTCAGCTCGGTGGCAATCTTCTTGTGACGTACCGTCAGCTCCTCAATGACTGGCAGGTTTTTGATGTGGGGGAAGAGGCTCCGGATAAGGTGCTGCGCCGAATTTATGTCAATCTAGAAACGCTCAAGCGCAATGGGGATAAATTTGCTACCAAGATTCAAGAGAAACTGAGGATAATTGTTGCAGGTGGTGATGGAACTGCTGGATGGCTTCTTGGAGTTGTTTCTGATCTGAAATTAGCTCATCCGCCACCGATAGCAACAGTACCCTTAGGAACTGGAAACAATCTTCCATTTGCATTTGGCTGGGGGAAGAAGAATCCTGGAACAGATCAACATGCTGTGGAGTCCTTTTTGCGTCAAGTAAAGGGAGCCAAGGAAATGAAGATAGACAGCTGGCACCTTCTCATGAGGATGAAGATTCCAAAAGGAGGTCCATTGGATCCCATTGCGCCTCTTGAGCTACCCCATTCTTTGCATGCCTTTGGCCGTGTCTCTGACACAAACAAGCTGAACATGGAGGGCTTCCATACATTTCGTGGGGGATTTTGGAATTACTTCAGTATGGGAATGGATGCTGAAGTATCATATGCGTTTCATTCAGAGCGGAAGTTGCATCCTGAAAAGTTTAAAAATCAGTTAGTTAATCAATCTACTTATGCAAAGCTTGGGTGTACACAAGGCTGGTTTTCTCCTTCCCTTTCTCAGAATTCTGCCCGGAATATAGCTCAATTTGCCAAGGTGATGATTATAAGAAACCATGGGGATCAATGGGAAGAGCTCTTCCTTCCTCACAGCATCAAGTCGATAGTCTGCCTCAATTTGCCTAGCTTTTCTGGTGGATTTAATCCTTGGGGAACACCACGTAGACGGAAGCATAATGCATTCACCCCTCCATATGTAGATGATGGCCATTTAGAGGTTGTAGGTTTTACTTCTGCTTGGCATGGACTTGTATTGCTTGCGCCAAATGGACATGGGACTCGTCTTGCTCAGGCCCACCGAATCAAATTTCTTTTTCACAAAGGTGCAATTGATCAGACGTACATGAGAATTGATGGGGAACCTTGGAGGCAACCCCTTCCATCTGATGATGACACTGTGATGGTGGAAATCTCTCACCTTGGCCAGGTCAACATTCTCGCTAACAATGGCTGCAGGTCTAAAAGTATGCACAATCCCTTAACACCTAGCGCTCAGGAAGAGGAAGGAGTTGACTCAGATGAAGAAAATCTTGAAGCAGAGTTCAGGAAGTTTGGTGCAGCAGACACATTCAAGCTTCCGGACGAAATTGATATTTCTCATCTTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001775 GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005886 GO:0006629 GO:0006793 GO:0006796 GO:0006950 GO:0007596 GO:0007599 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0030168 GO:0030258 GO:0030425 GO:0031974 GO:0031981 GO:0032501 GO:0032991 GO:0036477 GO:0042060 GO:0042995 GO:0043005 GO:0043197 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044255 GO:0044309 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044456 GO:0044463 GO:0044464 GO:0045202 GO:0046834 GO:0050817 GO:0050878 GO:0050896 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097447 GO:0097458 GO:0098794 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

485

Amino Acids

54.22

Weight (kDa)

6.68

Isoelectric Point (pI)

39.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	38 - 162	4.3e-20	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	231 - 404	3.6e-38	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	896
AarI	CACCTGC	1 cut(s)	320
Acc36I	ACCTGC	3 cut(s)	190, 320, 1293
AccB1I	GGYRCC	1 cut(s)	543
AccBSI	CCGCTC	1 cut(s)	751
AccI	GTMKAC	1 cut(s)	1012
AccII	CGCG	1 cut(s)	81
AccIII	TCCGGA	2 cut(s)	226, 1426
AciI	CCGC	2 cut(s)	392, 751
AclWI	GGATC	3 cut(s)	581, 594, 918
AcoI	YGGCCR	4 cut(s)	3, 631, 1054, 1270
AcsI	RAATTY	4 cut(s)	246, 287, 853, 1154
AcuI	CTGAAG	2 cut(s)	688, 744
AfaI	GTAC	5 cut(s)	60, 176, 411, 820, 1190
AfeI	AGCGCT	1 cut(s)	1339
AfiI	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
AgsI	TTSAA	6 cut(s)	34, 43, 65, 305, 1382, 1420
AhdI	GACNNNNNGTC	1 cut(s)	177
AjnI	CCWGG	2 cut(s)	466, 1272
AleI	CACNNNNGTG	1 cut(s)	1170
Alw21I	GWGCWC	1 cut(s)	927
Alw26I	GTCTC	1 cut(s)	643
AlwI	GGATC	3 cut(s)	581, 594, 918
Ama87I	CYCGRG	1 cut(s)	104
Aor13HI	TCCGGA	2 cut(s)	226, 1426
Aor51HI	AGCGCT	1 cut(s)	1339
AoxI	GGCC	5 cut(s)	3, 631, 1054, 1140, 1270
ApeKI	GCWGC	3 cut(s)	238, 1299, 1406
ApoI	RAATTY	4 cut(s)	246, 287, 853, 1154
Asp700I	GAANNNNTTC	2 cut(s)	38, 701
AspLEI	GCGC	6 cut(s)	81, 243, 276, 598, 1111, 1340
AspS9I	GGNCC	2 cut(s)	578, 1141
AsuC2I	CCSGG	1 cut(s)	862
AsuHPI	GGTGA	4 cut(s)	347, 898, 1024, 1256
AvaI	CYCGRG	1 cut(s)	104
AvaII	GGWCC	1 cut(s)	578
AxyI	CCTNAGG	1 cut(s)	415
BalI	TGGCCA	3 cut(s)	5, 1056, 1272
BamHI	GGATCC	1 cut(s)	586
BanI	GGYRCC	1 cut(s)	543
BanII	GRGCYC	2 cut(s)	105, 927
Bbv12I	GWGCWC	1 cut(s)	927
BbvI	GCAGC	3 cut(s)	225, 1286, 1418
BccI	CCATC	7 cut(s)	77, 332, 345, 1046, 1196, 1237, 1243
BceAI	ACGGC	1 cut(s)	618
BciT130I	CCWGG	2 cut(s)	468, 1274
BclI	TGATCA	1 cut(s)	1180
BcnI	CCSGG	1 cut(s)	862
BcoDI	GTCTC	1 cut(s)	643
BfaI	CTAG	3 cut(s)	260, 972, 1335
BfmI	CTRYAG	1 cut(s)	1300
BfoI	RGCGCY	1 cut(s)	1341
BfuAI	ACCTGC	3 cut(s)	190, 320, 1293
BisI	GCNGC	3 cut(s)	239, 1300, 1407
BlsI	GCNGC	3 cut(s)	240, 1301, 1408
Bme1390I	CCNGG	3 cut(s)	468, 862, 1274
Bme18I	GGWCC	1 cut(s)	578
BmeRI	GACNNNNNGTC	1 cut(s)	177
BmeT110I	CYCGRG	1 cut(s)	104
BmgT120I	GGNCC	2 cut(s)	578, 1141
BmiI	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
BmrFI	CCNGG	3 cut(s)	468, 862, 1274
BmsI	GCATC	3 cut(s)	709, 769, 951
Bpu10I	CCTNAGC	2 cut(s)	1137, 1341
BpuEI	CTTGAG	2 cut(s)	254, 623
BpuMI	CCSGG	1 cut(s)	862
BsaAI	YACGTR	1 cut(s)	1010
BsaJI	CCNNGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
BsaWI	WCCGGW	2 cut(s)	226, 1426
Bsc4I	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
Bse1I	ACTGG	3 cut(s)	113, 200, 427
Bse21I	CCTNAGG	1 cut(s)	415
Bse3DI	GCAATG	2 cut(s)	283, 591
BseAI	TCCGGA	2 cut(s)	226, 1426
BseBI	CCWGG	2 cut(s)	468, 1274
BseDI	CCNNGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
BseGI	GGATG	5 cut(s)	356, 388, 564, 724, 760
BseLI	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
BseMI	GCAATG	2 cut(s)	283, 591
BseMII	CTCAG	5 cut(s)	305, 863, 1151, 1355, 1377
BseNI	ACTGG	3 cut(s)	113, 200, 427
BseRI	GAGGAG	1 cut(s)	176
BseXI	GCAGC	3 cut(s)	225, 1286, 1418
BseYI	CCCAGC	1 cut(s)	450
BsgI	GTGCAG	1 cut(s)	1425
Bsh1236I	CGCG	1 cut(s)	81
BshFI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BshNI	GGYRCC	1 cut(s)	543
BsiHKAI	GWGCWC	1 cut(s)	927
BsiHKCI	CYCGRG	1 cut(s)	104
BsiSI	CCGG	3 cut(s)	227, 862, 1427
BslFI	GGGAC	1 cut(s)	1138
BslI	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
BsmAI	GTCTC	1 cut(s)	643
BsmFI	GGGAC	1 cut(s)	1138
BsmI	GAATGC	1 cut(s)	1028
BsnI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BsoBI	CYCGRG	1 cut(s)	104
Bsp1286I	GDGCHC	2 cut(s)	105, 927
Bsp13I	TCCGGA	2 cut(s)	226, 1426
Bsp1407I	TGTACA	1 cut(s)	818
Bsp143I	GATC	5 cut(s)	373, 475, 586, 910, 1180
Bsp19I	CCATGG	1 cut(s)	904
BspACI	CCGC	2 cut(s)	392, 751
BspANI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BspCNI	CTCAG	5 cut(s)	306, 862, 1150, 1354, 1376
BspEI	TCCGGA	2 cut(s)	226, 1426
BspFNI	CGCG	1 cut(s)	81
BspHI	TCATGA	1 cut(s)	552
BspLI	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
BspMAI	CTGCAG	1 cut(s)	1304
BspMI	ACCTGC	3 cut(s)	190, 320, 1293
BspPI	GGATC	3 cut(s)	581, 594, 918
BspQI	GCTCTTC	2 cut(s)	915, 932
BspT107I	GGYRCC	1 cut(s)	543
BsrBI	CCGCTC	1 cut(s)	751
BsrDI	GCAATG	2 cut(s)	283, 591
BsrGI	TGTACA	1 cut(s)	818
BsrI	ACTGG	3 cut(s)	113, 200, 427
BssECI	CCNNGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
BssMI	GATC	5 cut(s)	373, 475, 586, 910, 1180
BssT1I	CCWWGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
Bst2UI	CCWGG	2 cut(s)	468, 1274
Bst4CI	ACNGT	3 cut(s)	179, 409, 1246
Bst6I	CTCTTC	5 cut(s)	29, 213, 915, 932, 1341
BstAUI	TGTACA	1 cut(s)	818
BstBAI	YACGTR	1 cut(s)	1010
BstC8I	GCNNGC	3 cut(s)	542, 624, 1107
BstDEI	CTNAG	7 cut(s)	314, 415, 849, 1137, 1341, 1363, 1451
BstDSI	CCRYGG	1 cut(s)	904
BstENI	CCTNNNNNAGG	1 cut(s)	1271
BstF5I	GGATG	5 cut(s)	356, 388, 564, 724, 760
BstFNI	CGCG	1 cut(s)	81
BstH2I	RGCGCY	1 cut(s)	1341
BstHHI	GCGC	6 cut(s)	81, 243, 276, 598, 1111, 1340
BstKTI	GATC	5 cut(s)	376, 478, 589, 913, 1183
BstMAI	GTCTC	1 cut(s)	643
BstMBI	GATC	5 cut(s)	373, 475, 586, 910, 1180
BstMWI	GCNNNNNNNGC	3 cut(s)	604, 757, 878
BstNI	CCWGG	2 cut(s)	468, 1274
BstNSI	RCATGY	2 cut(s)	485, 626
BstSCI	CCNGG	3 cut(s)	466, 860, 1272
BstSFI	CTRYAG	1 cut(s)	1300
BstUI	CGCG	1 cut(s)	81
BstV1I	GCAGC	3 cut(s)	225, 1286, 1418
BstX2I	RGATCY	1 cut(s)	586
BstYI	RGATCY	1 cut(s)	586
Bsu36I	CCTNAGG	1 cut(s)	415
BsuRI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BtgI	CCRYGG	1 cut(s)	904
BtgZI	GCGATG	1 cut(s)	96
BtsCI	GGATG	5 cut(s)	356, 388, 564, 724, 760
BtsIMutI	CAGTG	1 cut(s)	1242
BveI	ACCTGC	3 cut(s)	190, 320, 1293
Cac8I	GCNNGC	3 cut(s)	542, 624, 1107
CciI	TCATGA	1 cut(s)	552
CfoI	GCGC	6 cut(s)	81, 243, 276, 598, 1111, 1340
Cfr13I	GGNCC	2 cut(s)	578, 1141
CseI	GACGC	1 cut(s)	491
Csp6I	GTAC	5 cut(s)	59, 175, 410, 819, 1189
CspCI	CAANNNNNGTGG	2 cut(s)	124, 159
CviAII	CATG	8 cut(s)	482, 553, 623, 661, 905, 1091, 1121, 1192
CviQI	GTAC	5 cut(s)	59, 175, 410, 819, 1189
DdeI	CTNAG	7 cut(s)	314, 415, 849, 1137, 1341, 1363, 1451
DpnI	GATC	5 cut(s)	375, 477, 588, 912, 1182
DpnII	GATC	5 cut(s)	373, 475, 586, 910, 1180
DraI	TTTAAA	1 cut(s)	775
DriI	GACNNNNNGTC	1 cut(s)	177
EaeI	YGGCCR	4 cut(s)	3, 631, 1054, 1270
Eam1104I	CTCTTC	5 cut(s)	29, 213, 915, 932, 1341
Eam1105I	GACNNNNNGTC	1 cut(s)	177
EarI	CTCTTC	5 cut(s)	29, 213, 915, 932, 1341
EciI	GGCGGA	1 cut(s)	381
Ecl136II	GAGCTC	1 cut(s)	925
Eco130I	CCWWGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
Eco24I	GRGCYC	2 cut(s)	105, 927
Eco47I	GGWCC	1 cut(s)	578
Eco47III	AGCGCT	1 cut(s)	1339
Eco53kI	GAGCTC	1 cut(s)	925
Eco57I	CTGAAG	2 cut(s)	688, 744
Eco81I	CCTNAGG	1 cut(s)	415
Eco88I	CYCGRG	1 cut(s)	104
EcoICRI	GAGCTC	1 cut(s)	925
EcoNI	CCTNNNNNAGG	1 cut(s)	1271
EcoRI	GAATTC	1 cut(s)	853
EcoRII	CCWGG	2 cut(s)	466, 1272
EcoT14I	CCWWGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
EcoT22I	ATGCAT	1 cut(s)	1030
EcoT38I	GRGCYC	2 cut(s)	105, 927
ErhI	CCWWGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
FaeI	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
FalI	AAGNNNNNCTT	2 cut(s)	1362, 1394
FaqI	GGGAC	1 cut(s)	1138
FatI	CATG	8 cut(s)	481, 552, 622, 660, 904, 1090, 1120, 1191
FauNDI	CATATG	2 cut(s)	733, 1042
FbaI	TGATCA	1 cut(s)	1180
FblI	GTMKAC	1 cut(s)	1012
Fnu4HI	GCNGC	3 cut(s)	239, 1300, 1407
FokI	GGATG	5 cut(s)	363, 375, 571, 731, 747
FriOI	GRGCYC	2 cut(s)	105, 927
Fsp4HI	GCNGC	3 cut(s)	239, 1300, 1407
FspBI	CTAG	3 cut(s)	260, 972, 1335
GlaI	GCGC	6 cut(s)	80, 242, 275, 597, 1110, 1339
GluI	GCNGC	3 cut(s)	239, 1300, 1407
GsaI	CCCAGC	1 cut(s)	454
HaeII	RGCGCY	1 cut(s)	1341
HaeIII	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
HapII	CCGG	3 cut(s)	227, 862, 1427
HgaI	GACGC	1 cut(s)	491
HhaI	GCGC	6 cut(s)	81, 243, 276, 598, 1111, 1340
Hin1II	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
Hin6I	GCGC	6 cut(s)	79, 241, 274, 596, 1109, 1338
HinP1I	GCGC	6 cut(s)	79, 241, 274, 596, 1109, 1338
HincII	GTYRAC	2 cut(s)	1279, 1360
HindII	GTYRAC	2 cut(s)	1279, 1360
HindIII	AAGCTT	2 cut(s)	809, 1421
HpaII	CCGG	3 cut(s)	227, 862, 1427
HphI	GGTGA	4 cut(s)	347, 898, 1024, 1256
Hpy166II	GTNNAC	5 cut(s)	819, 821, 1013, 1279, 1360
Hpy8I	GTNNAC	5 cut(s)	819, 821, 1013, 1279, 1360
HpyAV	CCTTC	5 cut(s)	557, 849, 941, 1235, 1346
HpyCH4III	ACNGT	3 cut(s)	179, 409, 1246
HpyCH4IV	ACGT	3 cut(s)	173, 1009, 1187
HpyF10VI	GCNNNNNNNGC	3 cut(s)	604, 757, 878
HpyF3I	CTNAG	7 cut(s)	314, 415, 849, 1137, 1341, 1363, 1451
HpySE526I	ACGT	3 cut(s)	173, 1009, 1187
Hsp92II	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
HspAI	GCGC	6 cut(s)	79, 241, 274, 596, 1109, 1338
Kpn2I	TCCGGA	2 cut(s)	226, 1426
Ksp22I	TGATCA	1 cut(s)	1180
Kzo9I	GATC	5 cut(s)	373, 475, 586, 910, 1180
LguI	GCTCTTC	2 cut(s)	915, 932
LmnI	GCTCC	3 cut(s)	189, 229, 515
Lsp1109I	GCAGC	3 cut(s)	225, 1286, 1418
LweI	GCATC	3 cut(s)	709, 769, 951
MaeI	CTAG	3 cut(s)	260, 972, 1335
MaeII	ACGT	3 cut(s)	173, 1009, 1187
MaeIII	GTNAC	1 cut(s)	169
MalI	GATC	5 cut(s)	375, 477, 588, 912, 1182
MbiI	CCGCTC	1 cut(s)	751
MboI	GATC	5 cut(s)	373, 475, 586, 910, 1180
MfeI	CAATTG	1 cut(s)	1176
MflI	RGATCY	1 cut(s)	586
MhlI	GDGCHC	2 cut(s)	105, 927
MlsI	TGGCCA	3 cut(s)	5, 1056, 1272
MluNI	TGGCCA	3 cut(s)	5, 1056, 1272
MlyI	GAGTC	3 cut(s)	500, 1120, 1355
Mox20I	TGGCCA	3 cut(s)	5, 1056, 1272
Mph1103I	ATGCAT	1 cut(s)	1030
MroI	TCCGGA	2 cut(s)	226, 1426
MroXI	GAANNNNTTC	2 cut(s)	38, 701
MscI	TGGCCA	3 cut(s)	5, 1056, 1272
MseI	TTAA	5 cut(s)	774, 789, 990, 1328, 1456
MslI	CAYNNNNRTG	3 cut(s)	557, 1170, 1247
Msp20I	TGGCCA	3 cut(s)	5, 1056, 1272
MspA1I	CMGCKG	1 cut(s)	540
MspI	CCGG	3 cut(s)	227, 862, 1427
MspR9I	CCNGG	3 cut(s)	468, 862, 1274
MunI	CAATTG	1 cut(s)	1176
Mva1269I	GAATGC	1 cut(s)	1028
MvaI	CCWGG	2 cut(s)	468, 1274
MvnI	CGCG	1 cut(s)	81
MwoI	GCNNNNNNNGC	3 cut(s)	604, 757, 878
NciI	CCSGG	1 cut(s)	862
NcoI	CCATGG	1 cut(s)	904
NdeI	CATATG	2 cut(s)	733, 1042
NdeII	GATC	5 cut(s)	373, 475, 586, 910, 1180
NlaIII	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
NlaIV	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
NmuCI	GTSAC	1 cut(s)	169
NsiI	ATGCAT	1 cut(s)	1030
NspI	RCATGY	2 cut(s)	485, 626
OliI	CACNNNNGTG	1 cut(s)	1170
PaeI	GCATGC	1 cut(s)	626
PagI	TCATGA	1 cut(s)	552
PaqCI	CACCTGC	1 cut(s)	320
PciSI	GCTCTTC	2 cut(s)	915, 932
PctI	GAATGC	1 cut(s)	1028
PdmI	GAANNNNTTC	2 cut(s)	38, 701
PfeI	GAWTC	7 cut(s)	46, 65, 71, 301, 463, 565, 1149
PfoI	TCCNGGA	1 cut(s)	466
PkrI	GCNGC	3 cut(s)	240, 1301, 1408
PleI	GAGTC	3 cut(s)	499, 1120, 1355
PpsI	GAGTC	3 cut(s)	499, 1120, 1355
Ppu21I	YACGTR	1 cut(s)	1010
PsiI	TTATAA	1 cut(s)	896
Psp124BI	GAGCTC	1 cut(s)	927
Psp6I	CCWGG	2 cut(s)	466, 1272
PspFI	CCCAGC	1 cut(s)	450
PspGI	CCWGG	2 cut(s)	466, 1272
PspN4I	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
PspPI	GGNCC	2 cut(s)	578, 1141
PstI	CTGCAG	1 cut(s)	1304
PsuI	RGATCY	1 cut(s)	586
PvuII	CAGCTG	1 cut(s)	540
RsaI	GTAC	5 cut(s)	60, 176, 411, 820, 1190
RsaNI	GTAC	5 cut(s)	59, 175, 410, 819, 1189
RseI	CAYNNNNRTG	3 cut(s)	557, 1170, 1247
SacI	GAGCTC	1 cut(s)	927
SapI	GCTCTTC	2 cut(s)	915, 932
SaqAI	TTAA	5 cut(s)	774, 789, 990, 1328, 1456
SatI	GCNGC	3 cut(s)	239, 1300, 1407
Sau3AI	GATC	5 cut(s)	373, 475, 586, 910, 1180
Sau96I	GGNCC	2 cut(s)	578, 1141
SchI	GAGTC	3 cut(s)	500, 1120, 1355
ScrFI	CCNGG	3 cut(s)	468, 862, 1274
SduI	GDGCHC	2 cut(s)	105, 927
SfaNI	GCATC	3 cut(s)	709, 769, 951
SfcI	CTRYAG	1 cut(s)	1300
SinI	GGWCC	1 cut(s)	578
SmiMI	CAYNNNNRTG	3 cut(s)	557, 1170, 1247
SmlI	CTYRAG	2 cut(s)	269, 602
SmoI	CTYRAG	2 cut(s)	269, 602
SphI	GCATGC	1 cut(s)	626
SsiI	CCGC	2 cut(s)	392, 751
SspMI	CTAG	3 cut(s)	260, 972, 1335
SstI	GAGCTC	1 cut(s)	927
StyD4I	CCNGG	3 cut(s)	466, 860, 1272
StyI	CCWWGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
TaaI	ACNGT	3 cut(s)	179, 409, 1246
TaiI	ACGT	3 cut(s)	176, 1012, 1190
TaqI	TCGA	1 cut(s)	950
TaqII	GACCGA	1 cut(s)	9
TatI	WGTACW	2 cut(s)	58, 818
TfiI	GAWTC	7 cut(s)	46, 65, 71, 301, 463, 565, 1149
Tru1I	TTAA	5 cut(s)	774, 789, 990, 1328, 1456
Tru9I	TTAA	5 cut(s)	774, 789, 990, 1328, 1456
TscAI	CASTG	1 cut(s)	1249
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	3 cut(s)	238, 1299, 1406
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	4 cut(s)	542, 575, 731, 1383
TspGWI	ACGGA	1 cut(s)	1030
TspRI	CASTG	1 cut(s)	1249
VpaK11BI	GGWCC	1 cut(s)	578
XagI	CCTNNNNNAGG	1 cut(s)	1271
XapI	RAATTY	4 cut(s)	246, 287, 853, 1154
XbaI	TCTAGA	1 cut(s)	259
XceI	RCATGY	2 cut(s)	485, 626
XcmI	CCANNNNNNNNNTGG	1 cut(s)	680
XmiI	GTMKAC	1 cut(s)	1012
XmnI	GAANNNNTTC	2 cut(s)	38, 701
XspI	CTAG	3 cut(s)	260, 972, 1335
Zsp2I	ATGCAT	1 cut(s)	1030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.