Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

30822133 .. 30827913

Gene Structure 5781 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG247800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1431 bp

ATGGTGGGAGATAGAGGCAAGTTGAAGGATTTTTACATTCCGAATTACATACTTGTGGGCGGGGCGGTGCCGAAGATCAAATTTGCACCTGCGTGTCCGGTGATAGTGTTTGTTAATACTAGAAGTGGTGGTCAGCTTGGAGGTGAACTTGTTCTTACATATAGAGCCCTTCTCAATGAGCACCAGGTATTTGACTTGGGACAAACGGCTCCTGACAAGGTGCTTCACAAGCTCTATGTTGCTTTGGAAACACTCAAGGGCAGCGGAGATGTTTTGGCTGCTGATATCCAGAAGAGGTTAAGAATAATTGCTGCAGGTGGAGATGGTACAGCTGGCTGGCTCCTCAGTGTAGTTTCTGATCTTAAACTGCCTAACCCACCTCCAATTGCCATGATGCCATTGGGAACTGGAAATAATGTCCATTTCGCGTTTGGATGGGGAAAGAAAAATCCTGGGACAGACCGACAATCTGTGCAGTATTTCTTGAACCAAGTGAAGACAGCAAAGGAAATGAAAATTGACAGCTGGCACATCATTATGAGAATGAGGACTCCAAAGGAAGGTTCATGTGATCCTATTGCACCTCTGGACCTACCGCATTCTTTGCATGCATTTCATCAGGAGGGCTTCCAGACATTTCGTGGGGGATTTTGGAACTACTTTAGCATGGGAATGGATGCTCAAATATCATATGATTTTCATGCTCAGAGAAAGTTGCACGCTGAAAAGTTTAACAACCAGTTAATTAATCAAAGTACTTACTTCAAGCTTGGATGTACTCAAGGATGGTTCTTTGCTTCTCTAATGCATCCTTCTTCAAGGAATATAGCACATCTGACAAAGATTAAGATCATGAAAAGACAAGGTCATTGGGAGGAGCTTCACATATCTCCCAGCATTAGGTCTATTGTTTGCCTCAATTTACCCAGTTTTTCTGGTGGTCTTGATCCATGGGGGAAGCCAAATAGAAAGAAACTGCGATATCGGGATTTAACTCCTCCATATGTGGATGATGGCCTTCTTGAGATTGTTGGTTTTAGAGATGCCTGGCATGGGCTTGCGTTACTTGCTCCTAAGGGACATGGGACTCGTCTTGCACAGGCACACGGAATACGTTTTGAGTTTTGCAAGGGCGCAGTCGACCATACATTCATGAGAATTGATGGGGAACCATGGAAACAACCTCTTCCAGTTGACGATGACACGGTGGTTGTAGAAATTTCTCACTTTGGCCAAGTAAGCATGCTTGCCACGCCATCGAAGCGATCCCAAAGTATATCTGACCCTGCGTCACCAGCTAGCTCCCATGATGACGATGATGATAGTAGTAATGAGGAAGAATATCCAGAAGATAGTGGAGAGAGAAGGAAGTTTGGTGCATCTGACACATTTAAAATCCCGGACGGGATTGACATTGCTCATTTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007610 GO:0007635 GO:0008150 GO:0009266 GO:0009605 GO:0009628 GO:0010646 GO:0023051 GO:0040011 GO:0042048 GO:0042221 GO:0042330 GO:0043052 GO:0048167 GO:0048168 GO:0048169 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0065007 GO:0065008 GO:0099177

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

476

Amino Acids

53.01

Weight (kDa)

7.75

Isoelectric Point (pI)

37.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	33 - 154	2e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	217 - 391	4e-36	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	97, 305
Acc36I	ACCTGC	2 cut(s)	97, 305
AccB1I	GGYRCC	1 cut(s)	67
AccI	GTMKAC	1 cut(s)	1140
AccII	CGCG	1 cut(s)	428
AciI	CCGC	4 cut(s)	60, 65, 264, 596
AclWI	GGATC	3 cut(s)	566, 941, 1260
AcoI	YGGCCR	1 cut(s)	1231
AcsI	RAATTY	2 cut(s)	80, 1218
AfaI	GTAC	3 cut(s)	328, 757, 778
AfiI	CCNNNNNNNGG	3 cut(s)	560, 900, 1053
AgsI	TTSAA	4 cut(s)	25, 487, 766, 819
AhdI	GACNNNNNGTC	1 cut(s)	1288
AjnI	CCWGG	3 cut(s)	183, 451, 1046
AjuI	GAANNNNNNNTTGG	2 cut(s)	547, 579
AleI	CACNNNNGTG	1 cut(s)	91
AluBI	AGCT	8 cut(s)	136, 232, 332, 525, 769, 880, 1298, 1302
AluI	AGCT	8 cut(s)	136, 232, 332, 525, 769, 880, 1298, 1302
Alw21I	GWGCWC	1 cut(s)	183
AlwI	GGATC	3 cut(s)	566, 941, 1260
AoxI	GGCC	2 cut(s)	1015, 1231
ApeKI	GCWGC	3 cut(s)	261, 278, 311
ApoI	RAATTY	2 cut(s)	80, 1218
AseI	ATTAAT	1 cut(s)	747
Asp700I	GAANNNNTTC	1 cut(s)	150
AspLEI	GCGC	1 cut(s)	1136
AspS9I	GGNCC	1 cut(s)	589
AsuC2I	CCSGG	1 cut(s)	1400
AsuHPI	GGTGA	3 cut(s)	112, 155, 1284
AsuNHI	GCTAGC	1 cut(s)	1298
AvaII	GGWCC	1 cut(s)	589
AxyI	CCTNAGG	1 cut(s)	1074
BalI	TGGCCA	1 cut(s)	1233
BanI	GGYRCC	1 cut(s)	67
BanII	GRGCYC	1 cut(s)	169
BarI	GAAGNNNNNNTAC	2 cut(s)	17, 49
BbsI	GAAGAC	1 cut(s)	503
Bbv12I	GWGCWC	1 cut(s)	183
BbvI	GCAGC	3 cut(s)	265, 273, 298
BccI	CCATC	6 cut(s)	317, 429, 780, 1007, 1157, 1264
BceAI	ACGGC	1 cut(s)	222
BciT130I	CCWGG	3 cut(s)	185, 453, 1048
BcnI	CCSGG	1 cut(s)	1400
BfaI	CTAG	2 cut(s)	120, 1299
BfmI	CTRYAG	1 cut(s)	312
BfuAI	ACCTGC	2 cut(s)	97, 305
BisI	GCNGC	3 cut(s)	262, 279, 312
BlsI	GCNGC	3 cut(s)	263, 280, 313
BmcAI	AGTACT	1 cut(s)	757
Bme1390I	CCNGG	4 cut(s)	185, 453, 1048, 1400
Bme18I	GGWCC	1 cut(s)	589
BmeRI	GACNNNNNGTC	1 cut(s)	1288
BmgT120I	GGNCC	1 cut(s)	589
BmiI	GGNNCC	4 cut(s)	69, 210, 341, 1170
BmrFI	CCNGG	4 cut(s)	185, 453, 1048, 1400
BmrI	ACTGGG	1 cut(s)	921
BmsI	GCATC	5 cut(s)	384, 667, 817, 1033, 1388
BmtI	GCTAGC	1 cut(s)	1302
BmuI	ACTGGG	1 cut(s)	921
BpiI	GAAGAC	1 cut(s)	503
BplI	GAGNNNNNCTC	2 cut(s)	156, 188
BpuEI	CTTGAG	3 cut(s)	239, 765, 1043
BpuMI	CCSGG	1 cut(s)	1400
BsaBI	GATNNNNATC	1 cut(s)	848
BsaJI	CCNNGG	3 cut(s)	452, 950, 1172
BsaWI	WCCGGW	1 cut(s)	97
Bsc4I	CCNNNNNNNGG	3 cut(s)	560, 900, 1053
Bse1I	ACTGG	4 cut(s)	412, 739, 927, 1190
Bse21I	CCTNAGG	1 cut(s)	1074
Bse3DI	GCAATG	1 cut(s)	1413
Bse8I	GATNNNNATC	1 cut(s)	848
BseBI	CCWGG	3 cut(s)	185, 453, 1048
BseDI	CCNNGG	3 cut(s)	452, 950, 1172
BseGI	GGATG	6 cut(s)	440, 682, 779, 791, 808, 1015
BseJI	GATNNNNATC	1 cut(s)	848
BseLI	CCNNNNNNNGG	3 cut(s)	560, 900, 1053
BseMI	GCAATG	1 cut(s)	1413
BseMII	CTCAG	2 cut(s)	358, 719
BseNI	ACTGG	4 cut(s)	412, 739, 927, 1190
BseRI	GAGGAG	3 cut(s)	332, 890, 987
BseXI	GCAGC	3 cut(s)	265, 273, 298
BseYI	CCCAGC	1 cut(s)	893
BsgI	GTGCAG	1 cut(s)	494
Bsh1236I	CGCG	1 cut(s)	428
BshFI	GGCC	2 cut(s)	1017, 1233
BshNI	GGYRCC	1 cut(s)	67
BsiHKAI	GWGCWC	1 cut(s)	183
BsiSI	CCGG	2 cut(s)	98, 1400
BslFI	GGGAC	4 cut(s)	213, 469, 1092, 1099
BslI	CCNNNNNNNGG	3 cut(s)	560, 900, 1053
BsmFI	GGGAC	4 cut(s)	213, 469, 1092, 1099
BsmI	GAATGC	1 cut(s)	598
BsnI	GGCC	2 cut(s)	1017, 1233
Bsp1286I	GDGCHC	2 cut(s)	169, 183
Bsp143I	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
Bsp19I	CCATGG	2 cut(s)	950, 1172
BspACI	CCGC	4 cut(s)	60, 65, 264, 596
BspANI	GGCC	2 cut(s)	1017, 1233
BspCNI	CTCAG	2 cut(s)	357, 718
BspFNI	CGCG	1 cut(s)	428
BspHI	TCATGA	2 cut(s)	852, 1152
BspLI	GGNNCC	4 cut(s)	69, 210, 341, 1170
BspMAI	CTGCAG	1 cut(s)	316
BspMI	ACCTGC	2 cut(s)	97, 305
BspOI	GCTAGC	1 cut(s)	1302
BspPI	GGATC	3 cut(s)	566, 941, 1260
BspT107I	GGYRCC	1 cut(s)	67
BsrDI	GCAATG	1 cut(s)	1413
BsrI	ACTGG	4 cut(s)	412, 739, 927, 1190
BssECI	CCNNGG	3 cut(s)	452, 950, 1172
BssMI	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
BssT1I	CCWWGG	2 cut(s)	950, 1172
Bst2UI	CCWGG	3 cut(s)	185, 453, 1048
Bst4CI	ACNGT	1 cut(s)	1207
Bst6I	CTCTTC	2 cut(s)	287, 1191
BstAPI	GCANNNNNTGC	1 cut(s)	604
BstC8I	GCNNGC	9 cut(s)	334, 338, 527, 609, 720, 1059, 1244, 1248, 1300
BstDEI	CTNAG	3 cut(s)	344, 705, 1074
BstDSI	CCRYGG	2 cut(s)	950, 1172
BstF5I	GGATG	6 cut(s)	440, 682, 779, 791, 808, 1015
BstFNI	CGCG	1 cut(s)	428
BstHHI	GCGC	1 cut(s)	1136
BstKTI	GATC	6 cut(s)	78, 361, 574, 852, 949, 1268
BstMBI	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
BstMWI	GCNNNNNNNGC	7 cut(s)	229, 604, 1067, 1239, 1252, 1261, 1295
BstNI	CCWGG	3 cut(s)	185, 453, 1048
BstNSI	RCATGY	2 cut(s)	611, 1246
BstSCI	CCNGG	4 cut(s)	183, 451, 1046, 1398
BstSFI	CTRYAG	1 cut(s)	312
BstUI	CGCG	1 cut(s)	428
BstV1I	GCAGC	3 cut(s)	265, 273, 298
BstV2I	GAAGAC	1 cut(s)	503
Bsu36I	CCTNAGG	1 cut(s)	1074
BsuRI	GGCC	2 cut(s)	1017, 1233
BtgI	CCRYGG	2 cut(s)	950, 1172
BtsCI	GGATG	6 cut(s)	440, 682, 779, 791, 808, 1015
BtsIMutI	CAGTG	1 cut(s)	352
BveI	ACCTGC	2 cut(s)	97, 305
Cac8I	GCNNGC	9 cut(s)	334, 338, 527, 609, 720, 1059, 1244, 1248, 1300
CciI	TCATGA	2 cut(s)	852, 1152
CfoI	GCGC	1 cut(s)	1136
Cfr13I	GGNCC	1 cut(s)	589
CseI	GACGC	1 cut(s)	1278
CsiI	ACCWGGT	1 cut(s)	183
Csp6I	GTAC	3 cut(s)	327, 756, 777
CviQI	GTAC	3 cut(s)	327, 756, 777
DdeI	CTNAG	3 cut(s)	344, 705, 1074
DpnI	GATC	6 cut(s)	77, 360, 573, 851, 948, 1267
DpnII	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
DraI	TTTAAA	1 cut(s)	1393
DriI	GACNNNNNGTC	1 cut(s)	1288
EaeI	YGGCCR	1 cut(s)	1231
Eam1104I	CTCTTC	2 cut(s)	287, 1191
Eam1105I	GACNNNNNGTC	1 cut(s)	1288
EarI	CTCTTC	2 cut(s)	287, 1191
Eco130I	CCWWGG	2 cut(s)	950, 1172
Eco24I	GRGCYC	1 cut(s)	169
Eco32I	GATATC	2 cut(s)	286, 983
Eco47I	GGWCC	1 cut(s)	589
Eco81I	CCTNAGG	1 cut(s)	1074
EcoRII	CCWGG	3 cut(s)	183, 451, 1046
EcoRV	GATATC	2 cut(s)	286, 983
EcoT14I	CCWWGG	2 cut(s)	950, 1172
EcoT22I	ATGCAT	2 cut(s)	613, 810
EcoT38I	GRGCYC	1 cut(s)	169
ErhI	CCWWGG	2 cut(s)	950, 1172
FaqI	GGGAC	4 cut(s)	213, 469, 1092, 1099
FauI	CCCGC	1 cut(s)	53
FauNDI	CATATG	2 cut(s)	691, 1003
FblI	GTMKAC	1 cut(s)	1140
Fnu4HI	GCNGC	3 cut(s)	262, 279, 312
FokI	GGATG	6 cut(s)	447, 689, 786, 795, 798, 1022
FriOI	GRGCYC	1 cut(s)	169
Fsp4HI	GCNGC	3 cut(s)	262, 279, 312
FspBI	CTAG	2 cut(s)	120, 1299
GlaI	GCGC	1 cut(s)	1135
GluI	GCNGC	3 cut(s)	262, 279, 312
GsaI	CCCAGC	1 cut(s)	897
HaeIII	GGCC	2 cut(s)	1017, 1233
HapII	CCGG	2 cut(s)	98, 1400
HgaI	GACGC	1 cut(s)	1278
HhaI	GCGC	1 cut(s)	1136
Hin6I	GCGC	1 cut(s)	1134
HinP1I	GCGC	1 cut(s)	1134
HincII	GTYRAC	2 cut(s)	1141, 1195
HindII	GTYRAC	2 cut(s)	1141, 1195
HindIII	AAGCTT	1 cut(s)	767
HinfI	GANTC	2 cut(s)	550, 1087
HpaII	CCGG	2 cut(s)	98, 1400
HphI	GGTGA	3 cut(s)	112, 155, 1284
Hpy166II	GTNNAC	3 cut(s)	146, 1141, 1195
Hpy188I	TCNGA	6 cut(s)	42, 358, 708, 837, 1282, 1384
Hpy8I	GTNNAC	3 cut(s)	146, 1141, 1195
HpyAV	CCTTC	6 cut(s)	19, 179, 554, 822, 1028, 1359
HpyCH4III	ACNGT	1 cut(s)	1207
HpyCH4IV	ACGT	1 cut(s)	1114
HpyF10VI	GCNNNNNNNGC	7 cut(s)	229, 604, 1067, 1239, 1252, 1261, 1295
HpyF3I	CTNAG	3 cut(s)	344, 705, 1074
HpySE526I	ACGT	1 cut(s)	1114
HspAI	GCGC	1 cut(s)	1134
Kzo9I	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
LmnI	GCTCC	5 cut(s)	214, 345, 877, 1075, 1307
Lsp1109I	GCAGC	3 cut(s)	265, 273, 298
LweI	GCATC	5 cut(s)	384, 667, 817, 1033, 1388
MabI	ACCWGGT	1 cut(s)	183
MaeI	CTAG	2 cut(s)	120, 1299
MaeII	ACGT	1 cut(s)	1114
MaeIII	GTNAC	2 cut(s)	1062, 1290
MalI	GATC	6 cut(s)	77, 360, 573, 851, 948, 1267
MboI	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
MboII	GAAGA	7 cut(s)	85, 304, 508, 807, 1178, 1349, 1361
MfeI	CAATTG	1 cut(s)	384
MhlI	GDGCHC	2 cut(s)	169, 183
MlsI	TGGCCA	1 cut(s)	1233
MluCI	AATT	9 cut(s)	43, 80, 306, 384, 516, 744, 919, 1158, 1218
MluNI	TGGCCA	1 cut(s)	1233
MlyI	GAGTC	2 cut(s)	544, 1081
Mox20I	TGGCCA	1 cut(s)	1233
Mph1103I	ATGCAT	2 cut(s)	613, 810
MroXI	GAANNNNTTC	1 cut(s)	150
MscI	TGGCCA	1 cut(s)	1233
MseI	TTAA	9 cut(s)	114, 299, 363, 732, 743, 747, 846, 992, 1392
MslI	CAYNNNNRTG	4 cut(s)	53, 91, 536, 671
Msp20I	TGGCCA	1 cut(s)	1233
MspA1I	CMGCKG	3 cut(s)	264, 332, 525
MspI	CCGG	2 cut(s)	98, 1400
MspR9I	CCNGG	4 cut(s)	185, 453, 1048, 1400
MunI	CAATTG	1 cut(s)	384
Mva1269I	GAATGC	1 cut(s)	598
MvaI	CCWGG	3 cut(s)	185, 453, 1048
MvnI	CGCG	1 cut(s)	428
MwoI	GCNNNNNNNGC	7 cut(s)	229, 604, 1067, 1239, 1252, 1261, 1295
NciI	CCSGG	1 cut(s)	1400
NcoI	CCATGG	2 cut(s)	950, 1172
NdeI	CATATG	2 cut(s)	691, 1003
NdeII	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
NheI	GCTAGC	1 cut(s)	1298
NlaIV	GGNNCC	4 cut(s)	69, 210, 341, 1170
NmuCI	GTSAC	1 cut(s)	1290
NsiI	ATGCAT	2 cut(s)	613, 810
NspI	RCATGY	2 cut(s)	611, 1246
OliI	CACNNNNGTG	1 cut(s)	91
PacI	TTAATTAA	1 cut(s)	747
PaeI	GCATGC	2 cut(s)	611, 1246
PagI	TCATGA	2 cut(s)	852, 1152
PaqCI	CACCTGC	2 cut(s)	97, 305
PctI	GAATGC	1 cut(s)	598
PdmI	GAANNNNTTC	1 cut(s)	150
PflFI	GACNNNGTC	1 cut(s)	864
PfoI	TCCNGGA	1 cut(s)	1398
PkrI	GCNGC	3 cut(s)	263, 280, 313
PleI	GAGTC	2 cut(s)	544, 1081
PpsI	GAGTC	2 cut(s)	544, 1081
PshBI	ATTAAT	1 cut(s)	747
Psp6I	CCWGG	3 cut(s)	183, 451, 1046
PspFI	CCCAGC	1 cut(s)	893
PspGI	CCWGG	3 cut(s)	183, 451, 1046
PspN4I	GGNNCC	4 cut(s)	69, 210, 341, 1170
PspPI	GGNCC	1 cut(s)	589
PstI	CTGCAG	1 cut(s)	316
PsyI	GACNNNGTC	1 cut(s)	864
PvuII	CAGCTG	2 cut(s)	332, 525
RsaI	GTAC	3 cut(s)	328, 757, 778
RsaNI	GTAC	3 cut(s)	327, 756, 777
RseI	CAYNNNNRTG	4 cut(s)	53, 91, 536, 671
SalI	GTCGAC	1 cut(s)	1139
SaqAI	TTAA	9 cut(s)	114, 299, 363, 732, 743, 747, 846, 992, 1392
SatI	GCNGC	3 cut(s)	262, 279, 312
Sau3AI	GATC	6 cut(s)	75, 358, 571, 849, 946, 1265
Sau96I	GGNCC	1 cut(s)	589
ScaI	AGTACT	1 cut(s)	757
SchI	GAGTC	2 cut(s)	544, 1081
ScrFI	CCNGG	4 cut(s)	185, 453, 1048, 1400
SduI	GDGCHC	2 cut(s)	169, 183
SexAI	ACCWGGT	1 cut(s)	183
SfaNI	GCATC	5 cut(s)	384, 667, 817, 1033, 1388
SfcI	CTRYAG	1 cut(s)	312
SinI	GGWCC	1 cut(s)	589
SmiMI	CAYNNNNRTG	4 cut(s)	53, 91, 536, 671
SmlI	CTYRAG	3 cut(s)	254, 780, 1022
SmoI	CTYRAG	3 cut(s)	254, 780, 1022
SphI	GCATGC	2 cut(s)	611, 1246
Sse9I	AATT	9 cut(s)	43, 80, 306, 384, 516, 744, 919, 1158, 1218
SsiI	CCGC	4 cut(s)	60, 65, 264, 596
SspMI	CTAG	2 cut(s)	120, 1299
StyD4I	CCNGG	4 cut(s)	183, 451, 1046, 1398
StyI	CCWWGG	2 cut(s)	950, 1172
TaaI	ACNGT	1 cut(s)	1207
TaiI	ACGT	1 cut(s)	1117
TaqI	TCGA	2 cut(s)	1140, 1259
TaqII	GACCGA	1 cut(s)	477
TasI	AATT	9 cut(s)	43, 80, 306, 384, 516, 744, 919, 1158, 1218
TatI	WGTACW	2 cut(s)	755, 776
Tru1I	TTAA	9 cut(s)	114, 299, 363, 732, 743, 747, 846, 992, 1392
Tru9I	TTAA	9 cut(s)	114, 299, 363, 732, 743, 747, 846, 992, 1392
TscAI	CASTG	1 cut(s)	352
TseFI	GTSAC	1 cut(s)	1290
TseI	GCWGC	3 cut(s)	261, 278, 311
Tsp45I	GTSAC	1 cut(s)	1290
TspDTI	ATGAA	6 cut(s)	527, 555, 605, 689, 869, 1141
TspGWI	ACGGA	1 cut(s)	1122
TspRI	CASTG	1 cut(s)	352
Tth111I	GACNNNGTC	1 cut(s)	864
VpaK11BI	GGWCC	1 cut(s)	589
VspI	ATTAAT	1 cut(s)	747
XapI	RAATTY	2 cut(s)	80, 1218
XceI	RCATGY	2 cut(s)	611, 1246
XcmI	CCANNNNNNNNNTGG	3 cut(s)	397, 428, 638
XmiI	GTMKAC	1 cut(s)	1140
XmnI	GAANNNNTTC	1 cut(s)	150
XspI	CTAG	2 cut(s)	120, 1299
ZrmI	AGTACT	1 cut(s)	757
Zsp2I	ATGCAT	2 cut(s)	613, 810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.