Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Reverse (-)

10741666 .. 10746268

Gene Structure 4603 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00088870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1458 bp

ATGGCCAAGTCCAGCTCTCGGTCTGATTTCTTGAAGAGTTTCAAGATTCCCAAGTATGTACTTGAATCCGAATCAAAGCGAGATGGGGAAGATTCTGATGAGCCCGAGTGTCCAGTTTTGGTGTTTATCAACTCCAAAAGTGGTGGTCAGCTCGGTGGCAATCTTCTTGTGACGTATCGTCAGCTCCTCAATGACTGGCAGGTTTTTGATGTGGGGGAAGAGGCTCCGGATAAGGTGCTGCGCCGAATTTATGTCAATCTAGAAACGCTCAAGCACAATGGGGATAAATTTGCTACCAAGATTCAAGAGAAACTGAGGATAATTGTTGCAGGTGGTGATGGAACTGCTGGCTGGCTTCTTGGAGTTGTTTCTGATTTGAAATTAGCTCACCCGCCACCGATAGCAACAGTACCCTTAGGAACTGGAAACAATCTTCCATTTGCATTTGGCTGGGGGAAGAAGAATCCTGGAACAGATCAACATGCTGTGGAGTCCTTTTTGCGTCAAGTAAAGGGAGCCAAGGAAATGAAGATAGACAGCTGGCACCTTCTCATGAGGATGAAGATTCCAAAAGGAGGTCCATTGGATCCCATTGCGCCTCTTGAGCTACCCCATTCTTTGCATGCCTTTGGCCGTGTCTCTGATACAAACGAGCTGAACATGGAGGGCTTCCATACATTTCGTGGGGGATTTTGGAATTACTTCAGTATGGGAATGGATGCCGAAGTATCATATGCGTTTCATTCAGAGCGGAAGTTGCATCCTGAAAAGTTTAAAAATCAGTTAGTTAATCAGTCTACTTATGCAAAGCTTGGGTGTACACAAGGCTGGTTTTCTCCTTCCCTTTCTCAGAATTCTGCCCGGAATATAGCTCAATTTGCCAAGGTGATGATTATAAAAAACCATGGGGATCAATGGGAAGAGCTCATCCTTCCTCACAGCATCAAGTCGATCGTCTGCCTCAATTTGCCTAGCTTTTCTGGTGGATTTAATCCTTGGGGAACACCACGTAGACGGAAGTATAATGCATTCACTCCTCCATATGTAGATGATGGCCATTTAGAGGTTGTAGGTTTTACTTCTGCTTGGCATGGACTTGTATTGCTTGCGCCAAATGGACATGGGACTCGTCTTGCTCAGGCCCACCGAATCAAATTTCTGTTTCACAAAGGTGCAATTGATCAGACGTACATGAGAATTGATGGGGAACCCTGGAGGCAACCCCTTCCATCTGATGATGACACTGTGATGGTGGAAATCTCTCACCTTGGCCAGGTCAACATTCTCGCTACCAATGGGTGCAGGTCTAAAAGTATGCACAATCCCTCAACACCTACAGCTCAGGAAGAGGAAGGAGTTGACTCAGATGAAGAAAATCTTGAAGCAGAGTTCAGGAAGTTCGGTGCAGCAGACACATTCAAGCTTCCGGACGAAATTGATATTTCTCATCTTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001775 GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005886 GO:0006629 GO:0006793 GO:0006796 GO:0006950 GO:0007596 GO:0007599 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009611 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0030168 GO:0030258 GO:0030425 GO:0031974 GO:0031981 GO:0032501 GO:0032991 GO:0036477 GO:0042060 GO:0042995 GO:0043005 GO:0043197 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044255 GO:0044309 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044456 GO:0044463 GO:0044464 GO:0045202 GO:0046834 GO:0050817 GO:0050878 GO:0050896 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071944 GO:0097447 GO:0097458 GO:0098794 GO:0120025 GO:0120038

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

485

Amino Acids

54.14

Weight (kDa)

6.45

Isoelectric Point (pI)

38.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	38 - 162	4.1e-20	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	231 - 404	6.5e-38	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	896
AarI	CACCTGC	1 cut(s)	320
Acc36I	ACCTGC	3 cut(s)	190, 320, 1293
AccB1I	GGYRCC	1 cut(s)	543
AccBSI	CCGCTC	1 cut(s)	751
AccI	GTMKAC	2 cut(s)	797, 1012
AccIII	TCCGGA	2 cut(s)	226, 1426
AciI	CCGC	2 cut(s)	392, 751
AclWI	GGATC	3 cut(s)	581, 594, 918
AcoI	YGGCCR	4 cut(s)	3, 631, 1054, 1270
AcsI	RAATTY	4 cut(s)	246, 287, 853, 1154
AcuI	CTGAAG	1 cut(s)	688
AfaI	GTAC	4 cut(s)	60, 411, 820, 1190
AfiI	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
AgsI	TTSAA	7 cut(s)	34, 43, 65, 305, 379, 1382, 1420
AhdI	GACNNNNNGTC	1 cut(s)	177
AjnI	CCWGG	3 cut(s)	466, 1211, 1272
AleI	CACNNNNGTG	1 cut(s)	1170
Alw21I	GWGCWC	1 cut(s)	927
Alw26I	GTCTC	1 cut(s)	643
AlwI	GGATC	3 cut(s)	581, 594, 918
Ama87I	CYCGRG	1 cut(s)	104
Aor13HI	TCCGGA	2 cut(s)	226, 1426
AoxI	GGCC	5 cut(s)	3, 631, 1054, 1140, 1270
ApeKI	GCWGC	2 cut(s)	238, 1406
ApoI	RAATTY	4 cut(s)	246, 287, 853, 1154
Asp700I	GAANNNNTTC	2 cut(s)	38, 701
AspLEI	GCGC	3 cut(s)	243, 598, 1111
AspS9I	GGNCC	2 cut(s)	578, 1141
AsuC2I	CCSGG	1 cut(s)	862
AsuHPI	GGTGA	4 cut(s)	347, 380, 898, 1256
AvaI	CYCGRG	1 cut(s)	104
AvaII	GGWCC	1 cut(s)	578
AxyI	CCTNAGG	1 cut(s)	415
BalI	TGGCCA	3 cut(s)	5, 1056, 1272
BamHI	GGATCC	1 cut(s)	586
BanI	GGYRCC	1 cut(s)	543
BanII	GRGCYC	2 cut(s)	105, 927
Bbv12I	GWGCWC	1 cut(s)	927
BbvI	GCAGC	2 cut(s)	225, 1418
BccI	CCATC	6 cut(s)	77, 332, 1046, 1196, 1237, 1243
BceAI	ACGGC	1 cut(s)	618
BciT130I	CCWGG	3 cut(s)	468, 1213, 1274
BclI	TGATCA	1 cut(s)	1180
BcnI	CCSGG	1 cut(s)	862
BcoDI	GTCTC	1 cut(s)	643
BfaI	CTAG	2 cut(s)	260, 972
BfmI	CTRYAG	1 cut(s)	1335
BfuAI	ACCTGC	3 cut(s)	190, 320, 1293
BisI	GCNGC	2 cut(s)	239, 1407
BlsI	GCNGC	2 cut(s)	240, 1408
Bme1390I	CCNGG	4 cut(s)	468, 862, 1213, 1274
Bme18I	GGWCC	1 cut(s)	578
BmeRI	GACNNNNNGTC	1 cut(s)	177
BmeT110I	CYCGRG	1 cut(s)	104
BmgT120I	GGNCC	2 cut(s)	578, 1141
BmiI	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
BmrFI	CCNGG	4 cut(s)	468, 862, 1213, 1274
BmsI	GCATC	3 cut(s)	709, 769, 951
BpmI	CTGGAG	1 cut(s)	1234
Bpu10I	CCTNAGC	2 cut(s)	1137, 1341
BpuEI	CTTGAG	2 cut(s)	254, 623
BpuMI	CCSGG	1 cut(s)	862
BsaAI	YACGTR	1 cut(s)	1010
BsaJI	CCNNGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
BsaWI	WCCGGW	2 cut(s)	226, 1426
Bsc4I	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
Bse1I	ACTGG	3 cut(s)	113, 200, 427
Bse21I	CCTNAGG	1 cut(s)	415
Bse3DI	GCAATG	1 cut(s)	591
BseAI	TCCGGA	2 cut(s)	226, 1426
BseBI	CCWGG	3 cut(s)	468, 1213, 1274
BseDI	CCNNGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
BseGI	GGATG	4 cut(s)	564, 724, 760, 927
BseLI	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
BseMI	GCAATG	1 cut(s)	591
BseMII	CTCAG	5 cut(s)	305, 863, 1151, 1355, 1377
BseNI	ACTGG	3 cut(s)	113, 200, 427
BseRI	GAGGAG	2 cut(s)	176, 1026
BseXI	GCAGC	2 cut(s)	225, 1418
BseYI	CCCAGC	1 cut(s)	450
BsgI	GTGCAG	2 cut(s)	1321, 1425
Bsh1285I	CGRYCG	1 cut(s)	954
BshFI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BshNI	GGYRCC	1 cut(s)	543
BsiEI	CGRYCG	1 cut(s)	954
BsiHKAI	GWGCWC	1 cut(s)	927
BsiHKCI	CYCGRG	1 cut(s)	104
BsiSI	CCGG	3 cut(s)	227, 862, 1427
BslFI	GGGAC	1 cut(s)	1138
BslI	CCNNNNNNNGG	4 cut(s)	18, 575, 1063, 1273
BsmAI	GTCTC	1 cut(s)	643
BsmFI	GGGAC	1 cut(s)	1138
BsmI	GAATGC	1 cut(s)	1028
BsnI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BsoBI	CYCGRG	1 cut(s)	104
Bsp1286I	GDGCHC	2 cut(s)	105, 927
Bsp13I	TCCGGA	2 cut(s)	226, 1426
Bsp1407I	TGTACA	1 cut(s)	818
Bsp143I	GATC	5 cut(s)	475, 586, 910, 951, 1180
Bsp19I	CCATGG	1 cut(s)	904
BspACI	CCGC	2 cut(s)	392, 751
BspANI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BspCNI	CTCAG	5 cut(s)	306, 862, 1150, 1354, 1376
BspEI	TCCGGA	2 cut(s)	226, 1426
BspHI	TCATGA	1 cut(s)	552
BspLI	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
BspMI	ACCTGC	3 cut(s)	190, 320, 1293
BspPI	GGATC	3 cut(s)	581, 594, 918
BspQI	GCTCTTC	1 cut(s)	915
BspT107I	GGYRCC	1 cut(s)	543
BsrBI	CCGCTC	1 cut(s)	751
BsrDI	GCAATG	1 cut(s)	591
BsrGI	TGTACA	1 cut(s)	818
BsrI	ACTGG	3 cut(s)	113, 200, 427
BssECI	CCNNGG	6 cut(s)	519, 882, 904, 995, 1211, 1267
BssMI	GATC	5 cut(s)	475, 586, 910, 951, 1180
BssT1I	CCWWGG	5 cut(s)	519, 882, 904, 995, 1267
Bst2UI	CCWGG	3 cut(s)	468, 1213, 1274
Bst4CI	ACNGT	2 cut(s)	409, 1246
Bst6I	CTCTTC	4 cut(s)	29, 213, 915, 1341
BstAUI	TGTACA	1 cut(s)	818
BstBAI	YACGTR	1 cut(s)	1010
BstC8I	GCNNGC	5 cut(s)	349, 353, 542, 624, 1107
BstDEI	CTNAG	7 cut(s)	314, 415, 849, 1137, 1341, 1363, 1451
BstDSI	CCRYGG	1 cut(s)	904
BstENI	CCTNNNNNAGG	1 cut(s)	1271
BstF5I	GGATG	4 cut(s)	564, 724, 760, 927
BstHHI	GCGC	3 cut(s)	243, 598, 1111
BstKTI	GATC	5 cut(s)	478, 589, 913, 954, 1183
BstMAI	GTCTC	1 cut(s)	643
BstMBI	GATC	5 cut(s)	475, 586, 910, 951, 1180
BstMCI	CGRYCG	1 cut(s)	954
BstMWI	GCNNNNNNNGC	3 cut(s)	604, 757, 878
BstNI	CCWGG	3 cut(s)	468, 1213, 1274
BstNSI	RCATGY	2 cut(s)	485, 626
BstSCI	CCNGG	4 cut(s)	466, 860, 1211, 1272
BstSFI	CTRYAG	1 cut(s)	1335
BstV1I	GCAGC	2 cut(s)	225, 1418
BstX2I	RGATCY	1 cut(s)	586
BstYI	RGATCY	1 cut(s)	586
Bsu36I	CCTNAGG	1 cut(s)	415
BsuRI	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
BtgI	CCRYGG	1 cut(s)	904
BtsCI	GGATG	4 cut(s)	564, 724, 760, 927
BtsIMutI	CAGTG	1 cut(s)	1242
BveI	ACCTGC	3 cut(s)	190, 320, 1293
Cac8I	GCNNGC	5 cut(s)	349, 353, 542, 624, 1107
CciI	TCATGA	1 cut(s)	552
CfoI	GCGC	3 cut(s)	243, 598, 1111
Cfr13I	GGNCC	2 cut(s)	578, 1141
CseI	GACGC	1 cut(s)	491
Csp6I	GTAC	4 cut(s)	59, 410, 819, 1189
CspCI	CAANNNNNGTGG	2 cut(s)	124, 159
CviAII	CATG	8 cut(s)	482, 553, 623, 661, 905, 1091, 1121, 1192
CviQI	GTAC	4 cut(s)	59, 410, 819, 1189
DdeI	CTNAG	7 cut(s)	314, 415, 849, 1137, 1341, 1363, 1451
DpnI	GATC	5 cut(s)	477, 588, 912, 953, 1182
DpnII	GATC	5 cut(s)	475, 586, 910, 951, 1180
DraI	TTTAAA	1 cut(s)	775
DriI	GACNNNNNGTC	1 cut(s)	177
EaeI	YGGCCR	4 cut(s)	3, 631, 1054, 1270
Eam1104I	CTCTTC	4 cut(s)	29, 213, 915, 1341
Eam1105I	GACNNNNNGTC	1 cut(s)	177
EarI	CTCTTC	4 cut(s)	29, 213, 915, 1341
Ecl136II	GAGCTC	1 cut(s)	925
Eco130I	CCWWGG	5 cut(s)	519, 882, 904, 995, 1267
Eco24I	GRGCYC	2 cut(s)	105, 927
Eco47I	GGWCC	1 cut(s)	578
Eco53kI	GAGCTC	1 cut(s)	925
Eco57I	CTGAAG	1 cut(s)	688
Eco81I	CCTNAGG	1 cut(s)	415
Eco88I	CYCGRG	1 cut(s)	104
EcoICRI	GAGCTC	1 cut(s)	925
EcoNI	CCTNNNNNAGG	1 cut(s)	1271
EcoRI	GAATTC	1 cut(s)	853
EcoRII	CCWGG	3 cut(s)	466, 1211, 1272
EcoT14I	CCWWGG	5 cut(s)	519, 882, 904, 995, 1267
EcoT22I	ATGCAT	1 cut(s)	1030
EcoT38I	GRGCYC	2 cut(s)	105, 927
ErhI	CCWWGG	5 cut(s)	519, 882, 904, 995, 1267
FaeI	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
FalI	AAGNNNNNCTT	2 cut(s)	1362, 1394
FaqI	GGGAC	1 cut(s)	1138
FatI	CATG	8 cut(s)	481, 552, 622, 660, 904, 1090, 1120, 1191
FauI	CCCGC	1 cut(s)	399
FauNDI	CATATG	2 cut(s)	733, 1042
FbaI	TGATCA	1 cut(s)	1180
FblI	GTMKAC	2 cut(s)	797, 1012
Fnu4HI	GCNGC	2 cut(s)	239, 1407
FokI	GGATG	4 cut(s)	571, 731, 747, 914
FriOI	GRGCYC	2 cut(s)	105, 927
Fsp4HI	GCNGC	2 cut(s)	239, 1407
FspBI	CTAG	2 cut(s)	260, 972
GlaI	GCGC	3 cut(s)	242, 597, 1110
GluI	GCNGC	2 cut(s)	239, 1407
GsaI	CCCAGC	1 cut(s)	454
GsuI	CTGGAG	1 cut(s)	1234
HaeIII	GGCC	5 cut(s)	5, 633, 1056, 1142, 1272
HapII	CCGG	3 cut(s)	227, 862, 1427
HgaI	GACGC	1 cut(s)	491
HhaI	GCGC	3 cut(s)	243, 598, 1111
Hin1II	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
Hin6I	GCGC	3 cut(s)	241, 596, 1109
HinP1I	GCGC	3 cut(s)	241, 596, 1109
HincII	GTYRAC	2 cut(s)	1279, 1360
HindII	GTYRAC	2 cut(s)	1279, 1360
HindIII	AAGCTT	2 cut(s)	809, 1421
HpaII	CCGG	3 cut(s)	227, 862, 1427
HphI	GGTGA	4 cut(s)	347, 380, 898, 1256
Hpy166II	GTNNAC	6 cut(s)	798, 819, 821, 1013, 1279, 1360
Hpy8I	GTNNAC	6 cut(s)	798, 819, 821, 1013, 1279, 1360
HpyAV	CCTTC	5 cut(s)	557, 849, 941, 1235, 1346
HpyCH4III	ACNGT	2 cut(s)	409, 1246
HpyCH4IV	ACGT	3 cut(s)	173, 1009, 1187
HpyF10VI	GCNNNNNNNGC	3 cut(s)	604, 757, 878
HpyF3I	CTNAG	7 cut(s)	314, 415, 849, 1137, 1341, 1363, 1451
HpySE526I	ACGT	3 cut(s)	173, 1009, 1187
Hsp92II	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
HspAI	GCGC	3 cut(s)	241, 596, 1109
Kpn2I	TCCGGA	2 cut(s)	226, 1426
Ksp22I	TGATCA	1 cut(s)	1180
Kzo9I	GATC	5 cut(s)	475, 586, 910, 951, 1180
LguI	GCTCTTC	1 cut(s)	915
LmnI	GCTCC	3 cut(s)	189, 229, 515
Lsp1109I	GCAGC	2 cut(s)	225, 1418
LweI	GCATC	3 cut(s)	709, 769, 951
MaeI	CTAG	2 cut(s)	260, 972
MaeII	ACGT	3 cut(s)	173, 1009, 1187
MaeIII	GTNAC	1 cut(s)	169
MalI	GATC	5 cut(s)	477, 588, 912, 953, 1182
MbiI	CCGCTC	1 cut(s)	751
MboI	GATC	5 cut(s)	475, 586, 910, 951, 1180
MfeI	CAATTG	1 cut(s)	1176
MflI	RGATCY	1 cut(s)	586
MhlI	GDGCHC	2 cut(s)	105, 927
MlsI	TGGCCA	3 cut(s)	5, 1056, 1272
MluNI	TGGCCA	3 cut(s)	5, 1056, 1272
MlyI	GAGTC	3 cut(s)	500, 1120, 1355
Mox20I	TGGCCA	3 cut(s)	5, 1056, 1272
Mph1103I	ATGCAT	1 cut(s)	1030
MroI	TCCGGA	2 cut(s)	226, 1426
MroXI	GAANNNNTTC	2 cut(s)	38, 701
MscI	TGGCCA	3 cut(s)	5, 1056, 1272
MseI	TTAA	4 cut(s)	774, 789, 990, 1456
MslI	CAYNNNNRTG	3 cut(s)	557, 1170, 1247
Msp20I	TGGCCA	3 cut(s)	5, 1056, 1272
MspA1I	CMGCKG	1 cut(s)	540
MspI	CCGG	3 cut(s)	227, 862, 1427
MspR9I	CCNGG	4 cut(s)	468, 862, 1213, 1274
MunI	CAATTG	1 cut(s)	1176
Mva1269I	GAATGC	1 cut(s)	1028
MvaI	CCWGG	3 cut(s)	468, 1213, 1274
MwoI	GCNNNNNNNGC	3 cut(s)	604, 757, 878
NciI	CCSGG	1 cut(s)	862
NcoI	CCATGG	1 cut(s)	904
NdeI	CATATG	2 cut(s)	733, 1042
NdeII	GATC	5 cut(s)	475, 586, 910, 951, 1180
NlaIII	CATG	8 cut(s)	485, 556, 626, 664, 908, 1094, 1124, 1195
NlaIV	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
NmuCI	GTSAC	1 cut(s)	169
NsiI	ATGCAT	1 cut(s)	1030
NspI	RCATGY	2 cut(s)	485, 626
OliI	CACNNNNGTG	1 cut(s)	1170
PaeI	GCATGC	1 cut(s)	626
PagI	TCATGA	1 cut(s)	552
PaqCI	CACCTGC	1 cut(s)	320
PciSI	GCTCTTC	1 cut(s)	915
PctI	GAATGC	1 cut(s)	1028
PdmI	GAANNNNTTC	2 cut(s)	38, 701
PfeI	GAWTC	8 cut(s)	46, 65, 71, 92, 301, 463, 565, 1149
PfoI	TCCNGGA	1 cut(s)	466
PkrI	GCNGC	2 cut(s)	240, 1408
Ple19I	CGATCG	1 cut(s)	954
PleI	GAGTC	3 cut(s)	499, 1120, 1355
PpsI	GAGTC	3 cut(s)	499, 1120, 1355
Ppu21I	YACGTR	1 cut(s)	1010
PsiI	TTATAA	1 cut(s)	896
Psp124BI	GAGCTC	1 cut(s)	927
Psp6I	CCWGG	3 cut(s)	466, 1211, 1272
PspFI	CCCAGC	1 cut(s)	450
PspGI	CCWGG	3 cut(s)	466, 1211, 1272
PspN4I	GGNNCC	5 cut(s)	225, 517, 545, 588, 1209
PspPI	GGNCC	2 cut(s)	578, 1141
PsuI	RGATCY	1 cut(s)	586
PvuI	CGATCG	1 cut(s)	954
PvuII	CAGCTG	1 cut(s)	540
RsaI	GTAC	4 cut(s)	60, 411, 820, 1190
RsaNI	GTAC	4 cut(s)	59, 410, 819, 1189
RseI	CAYNNNNRTG	3 cut(s)	557, 1170, 1247
SacI	GAGCTC	1 cut(s)	927
SapI	GCTCTTC	1 cut(s)	915
SaqAI	TTAA	4 cut(s)	774, 789, 990, 1456
SatI	GCNGC	2 cut(s)	239, 1407
Sau3AI	GATC	5 cut(s)	475, 586, 910, 951, 1180
Sau96I	GGNCC	2 cut(s)	578, 1141
SchI	GAGTC	3 cut(s)	500, 1120, 1355
ScrFI	CCNGG	4 cut(s)	468, 862, 1213, 1274
SduI	GDGCHC	2 cut(s)	105, 927
SfaNI	GCATC	3 cut(s)	709, 769, 951
SfcI	CTRYAG	1 cut(s)	1335
SinI	GGWCC	1 cut(s)	578
SmiMI	CAYNNNNRTG	3 cut(s)	557, 1170, 1247
SmlI	CTYRAG	2 cut(s)	269, 602
SmoI	CTYRAG	2 cut(s)	269, 602
SphI	GCATGC	1 cut(s)	626
SsiI	CCGC	2 cut(s)	392, 751
SspMI	CTAG	2 cut(s)	260, 972
SstI	GAGCTC	1 cut(s)	927
StyD4I	CCNGG	4 cut(s)	466, 860, 1211, 1272
StyI	CCWWGG	5 cut(s)	519, 882, 904, 995, 1267
TaaI	ACNGT	2 cut(s)	409, 1246
TaiI	ACGT	3 cut(s)	176, 1012, 1190
TaqI	TCGA	1 cut(s)	950
TaqII	GACCGA	1 cut(s)	9
TatI	WGTACW	2 cut(s)	58, 818
TfiI	GAWTC	8 cut(s)	46, 65, 71, 92, 301, 463, 565, 1149
Tru1I	TTAA	4 cut(s)	774, 789, 990, 1456
Tru9I	TTAA	4 cut(s)	774, 789, 990, 1456
TscAI	CASTG	1 cut(s)	1249
TseFI	GTSAC	1 cut(s)	169
TseI	GCWGC	2 cut(s)	238, 1406
Tsp45I	GTSAC	1 cut(s)	169
TspDTI	ATGAA	4 cut(s)	542, 575, 731, 1383
TspGWI	ACGGA	1 cut(s)	1030
TspRI	CASTG	1 cut(s)	1249
VpaK11BI	GGWCC	1 cut(s)	578
XagI	CCTNNNNNAGG	1 cut(s)	1271
XapI	RAATTY	4 cut(s)	246, 287, 853, 1154
XbaI	TCTAGA	1 cut(s)	259
XceI	RCATGY	2 cut(s)	485, 626
XcmI	CCANNNNNNNNNTGG	1 cut(s)	680
XmiI	GTMKAC	2 cut(s)	797, 1012
XmnI	GAANNNNTTC	2 cut(s)	38, 701
XspI	CTAG	2 cut(s)	260, 972
Zsp2I	ATGCAT	1 cut(s)	1030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.