Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0001034

Physical Location & Seq

Genomic Coordinates Forward (+)

63562308 .. 63568625

Gene Structure 6318 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00044560.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGGAGTTCCGGAATCGAATTTCCGGTTGTCGCTTGCGAGACTCTATACACGGTCTCGTTTCGAGGAGTTGGGAGGCAATGGTGGGCGATAGAGGCAAGTTGAAGGATTTTTACATTCCGAATTACATACTTGTGGGCGGGGCGATGCCGAAGATCAAATTTGCGCCTGCGTGTCCGGTGATAGTGTTTGTTAATACTAGAAGTGGTGGTCAGCTTGGAGGTGAACTTCTTCTTACATATAGAGCCCTTCTCAATGAGCACCAGGTGTTTGACTTGGGACAAACGGCTCCTGACAAGGTGCTTCACAAGCTCTATGTTGCTTTGGAAACACTCAAGGGCAGCGGAGATGTTTTGGCTGCTGATATCCAGAAGAGGTTAAGAATAATTGCTGCAGGTGGAGATGGTACAGCTGGTTGGCTCCTCAGTGTAGTTTCTGATCTTAAACTGCCTAACCCACCTCCAATTGCCACGATGCCATTGGGAACTGGAAATAATGTCCATTTCGCGTTTGGATGGGGAAAGAAAAATCCTGGGACAGACCGACAATCTGTGCAGTATTTCTTGAACCAAGTGAAGACAGCAAAGGAAATGCAAATTGACAGCTGGCACATCATTATGAGAATGAGGACTCCAAAGGAAGGTTCATGTGATCCTATTGCACCTCTGGACCTACCGCATTCTTTGCATGCATTTCATCAGGTCTCCAAAACTGATACACTCAATCTGGAGGGTTTCCAGACATTTCGTGGGGGATTTTGGAACTACTTTAGCATGGGAATGGATGCTCAAATATCATATGATTTTCATGCTCAGAGAAAGTTGCACGCTGAAAAGTTTAACAACCAGTTAATTAATCAAAGTACTTACTTCAAGCTTGGATGTACTCAAGGATGGTTCTTTGCTTCTCTAATGCATCCTTCTTCAAGGAATATAGCACATCTGACAAAGATTAAGATCATGAAAAGACAAGGTCATTGGGAGGAGCTTCACATATCTCCCAGCATTAGGTCTATTGTTTGCCTCAATTTACCCAGTTTTTCTGGTGGTCTTGATCCATGGGGGAAGCCAAATAGAAAGAAACTGCGATATCGGGATTTAACTCCTCCATATGTGGATGATGGCCTTCTTGAGATTGTTGGTTTTAGAGATGCCTGGCATGGGCTTGCGTTACTTGCTCCTAAGGGACATGGGACTCGTCTTGCACAAGCACACGGAATACGTTTTGAGTTTTGCAAGGGCGCAGTCGACCATACATTCATGAGAATTGATGGGGAACCATGGAAACAACCTCTTCCAGTTGACGATGACACGGTGGTTGTAGAAATTTCTCACTTTGGCCAATTAAGCATGCTTGCCACGCCATCGAAGCGATCCAAAAGTATATCTGACCCTGCGTCACCAGCTAGTTCCCATGATGACGATGATGATAGTAGTAATGAGGAAGAATATCCAGAAGACAGTGGAGAGAGAAAGAAGTTTGGTGCATCTGACACATTTAAAATCCCGGACGGGATTGACATTGCTCATTTCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0007610 GO:0007635 GO:0008150 GO:0009266 GO:0009605 GO:0009628 GO:0010646 GO:0023051 GO:0040011 GO:0042048 GO:0042221 GO:0042330 GO:0043052 GO:0048167 GO:0048168 GO:0048169 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0065007 GO:0065008 GO:0099177

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

511

Amino Acids

57.05

Weight (kDa)

8.37

Isoelectric Point (pI)

35.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	59 - 179	3.5e-19	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	252 - 426	4.6e-36	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001034)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G20900 AT2G20900 AT2G20900 AT2G20900
fragaria_vesca	FvH4_3g21140 FvH4_3g21140 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410 FvH4_6g44410
malus_domestica	MD09G1097300.v1.1 MD11G1235100.v1.1 MD17G1085600.v1.1 MD17G1085700.v1.1
prunus_persica	Prupe.3G228600_v2.0.a1 Prupe.3G228600_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1 Prupe.4G191900_v2.0.a1
pyrus_communis	pycom09g02160 pycom11g20670 pycom17g08330
rosa_chinensis	RchiOBHm_Chr2g0161401 RchiOBHm_Chr5g0036201
rosa_laevigata	RLG00000008429 RLG00000020681 RLG00000021281 RLG00000022547 RLG00000033679
rosa_multiflora	Rmu_co8358135.1_g000001 Rmu_sc0000247.1_g000005 Rmu_sc0003548.1_g000003 Rmu_sc0040460.1_g000002
rosa_roxburghii	Rroxscaffold_1G00044560 Rroxscaffold_2G00088870
rosa_rugosa	Rorug02G0489000 Rorug02G0489100 Rorug05G0153500 Rorug05G0153600.1 Rorug05G0153700 Rorug05G0153800 Rorug05G0154100
rosa_samantha	Rh2AG555300 Rh2BG568500 Rh2CG539200 Rh2DG578100 Rh3CG359800 Rh3DG363000 Rh5AG247800 Rh5BG248400 Rh5CG279700 Rh5CG492000 Rh5DG256700 Rh6CG191300
rosa_wichuraiana	Rw0G008460 Rw2G045940 Rw3G028650 Rw5G022720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	383
Acc36I	ACCTGC	1 cut(s)	383
AccI	GTMKAC	1 cut(s)	1245
AccII	CGCG	1 cut(s)	506
AccIII	TCCGGA	1 cut(s)	9
AciI	CCGC	3 cut(s)	138, 342, 674
AclWI	GGATC	3 cut(s)	644, 1046, 1365
AcoI	YGGCCR	1 cut(s)	1336
AcsI	RAATTY	3 cut(s)	18, 158, 1323
AdeI	CACNNNGTG	1 cut(s)	265
AfaI	GTAC	3 cut(s)	406, 862, 883
AfiI	CCNNNNNNNGG	3 cut(s)	638, 1005, 1158
AgsI	TTSAA	4 cut(s)	103, 565, 871, 924
AhdI	GACNNNNNGTC	1 cut(s)	1393
AjnI	CCWGG	3 cut(s)	261, 529, 1151
AjuI	GAANNNNNNNTTGG	2 cut(s)	625, 657
AluBI	AGCT	7 cut(s)	214, 310, 410, 603, 874, 985, 1403
AluI	AGCT	7 cut(s)	214, 310, 410, 603, 874, 985, 1403
Alw21I	GWGCWC	1 cut(s)	261
Alw26I	GTCTC	3 cut(s)	33, 59, 706
AlwI	GGATC	3 cut(s)	644, 1046, 1365
Aor13HI	TCCGGA	1 cut(s)	9
AoxI	GGCC	2 cut(s)	1120, 1336
ApeKI	GCWGC	3 cut(s)	339, 356, 389
ApoI	RAATTY	3 cut(s)	18, 158, 1323
AseI	ATTAAT	1 cut(s)	852
Asp700I	GAANNNNTTC	1 cut(s)	228
AspLEI	GCGC	2 cut(s)	166, 1241
AspS9I	GGNCC	1 cut(s)	667
AsuC2I	CCSGG	1 cut(s)	1505
AsuHPI	GGTGA	3 cut(s)	190, 233, 1389
AvaII	GGWCC	1 cut(s)	667
AxyI	CCTNAGG	1 cut(s)	1179
BalI	TGGCCA	1 cut(s)	1338
BanII	GRGCYC	1 cut(s)	247
BarI	GAAGNNNNNNTAC	2 cut(s)	95, 127
BbsI	GAAGAC	2 cut(s)	581, 1461
Bbv12I	GWGCWC	1 cut(s)	261
BbvI	GCAGC	3 cut(s)	343, 351, 376
BccI	CCATC	6 cut(s)	395, 507, 885, 1112, 1262, 1369
BceAI	ACGGC	1 cut(s)	300
BciT130I	CCWGG	3 cut(s)	263, 531, 1153
BcnI	CCSGG	1 cut(s)	1505
BcoDI	GTCTC	3 cut(s)	33, 59, 706
BfaI	CTAG	2 cut(s)	198, 1404
BfmI	CTRYAG	1 cut(s)	390
BfuAI	ACCTGC	1 cut(s)	383
BisI	GCNGC	3 cut(s)	340, 357, 390
BlsI	GCNGC	3 cut(s)	341, 358, 391
BmcAI	AGTACT	1 cut(s)	862
Bme1390I	CCNGG	4 cut(s)	263, 531, 1153, 1505
Bme18I	GGWCC	1 cut(s)	667
BmeRI	GACNNNNNGTC	1 cut(s)	1393
BmgT120I	GGNCC	1 cut(s)	667
BmiI	GGNNCC	3 cut(s)	288, 419, 1275
BmrFI	CCNGG	4 cut(s)	263, 531, 1153, 1505
BmrI	ACTGGG	1 cut(s)	1026
BmsI	GCATC	6 cut(s)	135, 462, 772, 922, 1138, 1493
BmuI	ACTGGG	1 cut(s)	1026
BpiI	GAAGAC	2 cut(s)	581, 1461
BplI	GAGNNNNNCTC	2 cut(s)	234, 266
BpmI	CTGGAG	1 cut(s)	746
BpuEI	CTTGAG	3 cut(s)	317, 870, 1148
BpuMI	CCSGG	1 cut(s)	1505
BsaBI	GATNNNNATC	1 cut(s)	953
BsaI	GGTCTC	2 cut(s)	59, 706
BsaJI	CCNNGG	3 cut(s)	530, 1055, 1277
BsaWI	WCCGGW	3 cut(s)	9, 23, 175
Bsc4I	CCNNNNNNNGG	3 cut(s)	638, 1005, 1158
Bse1I	ACTGG	4 cut(s)	490, 844, 1032, 1295
Bse21I	CCTNAGG	1 cut(s)	1179
Bse3DI	GCAATG	2 cut(s)	84, 1518
Bse8I	GATNNNNATC	1 cut(s)	953
BseAI	TCCGGA	1 cut(s)	9
BseBI	CCWGG	3 cut(s)	263, 531, 1153
BseDI	CCNNGG	3 cut(s)	530, 1055, 1277
BseGI	GGATG	6 cut(s)	518, 787, 884, 896, 913, 1120
BseJI	GATNNNNATC	1 cut(s)	953
BseLI	CCNNNNNNNGG	3 cut(s)	638, 1005, 1158
BseMI	GCAATG	2 cut(s)	84, 1518
BseMII	CTCAG	2 cut(s)	436, 824
BseNI	ACTGG	4 cut(s)	490, 844, 1032, 1295
BseRI	GAGGAG	4 cut(s)	79, 410, 995, 1092
BseXI	GCAGC	3 cut(s)	343, 351, 376
BseYI	CCCAGC	1 cut(s)	998
BsgI	GTGCAG	1 cut(s)	572
Bsh1236I	CGCG	1 cut(s)	506
BshFI	GGCC	2 cut(s)	1122, 1338
BsiHKAI	GWGCWC	1 cut(s)	261
BsiSI	CCGG	4 cut(s)	10, 24, 176, 1505
BslFI	GGGAC	4 cut(s)	291, 547, 1197, 1204
BslI	CCNNNNNNNGG	3 cut(s)	638, 1005, 1158
BsmAI	GTCTC	3 cut(s)	33, 59, 706
BsmFI	GGGAC	4 cut(s)	291, 547, 1197, 1204
BsmI	GAATGC	1 cut(s)	676
BsnI	GGCC	2 cut(s)	1122, 1338
Bso31I	GGTCTC	2 cut(s)	59, 706
Bsp1286I	GDGCHC	2 cut(s)	247, 261
Bsp13I	TCCGGA	1 cut(s)	9
Bsp143I	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
Bsp19I	CCATGG	2 cut(s)	1055, 1277
BspACI	CCGC	3 cut(s)	138, 342, 674
BspANI	GGCC	2 cut(s)	1122, 1338
BspCNI	CTCAG	2 cut(s)	435, 823
BspEI	TCCGGA	1 cut(s)	9
BspFNI	CGCG	1 cut(s)	506
BspHI	TCATGA	2 cut(s)	957, 1257
BspLI	GGNNCC	3 cut(s)	288, 419, 1275
BspMAI	CTGCAG	1 cut(s)	394
BspMI	ACCTGC	1 cut(s)	383
BspPI	GGATC	3 cut(s)	644, 1046, 1365
BspTNI	GGTCTC	2 cut(s)	59, 706
BsrDI	GCAATG	2 cut(s)	84, 1518
BsrI	ACTGG	4 cut(s)	490, 844, 1032, 1295
BssECI	CCNNGG	3 cut(s)	530, 1055, 1277
BssMI	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
BssT1I	CCWWGG	2 cut(s)	1055, 1277
Bst2UI	CCWGG	3 cut(s)	263, 531, 1153
Bst4CI	ACNGT	3 cut(s)	53, 1312, 1460
Bst6I	CTCTTC	2 cut(s)	365, 1296
BstAPI	GCANNNNNTGC	1 cut(s)	682
BstC8I	GCNNGC	8 cut(s)	35, 168, 605, 687, 825, 1164, 1349, 1353
BstDEI	CTNAG	3 cut(s)	422, 810, 1179
BstDSI	CCRYGG	2 cut(s)	1055, 1277
BstF5I	GGATG	6 cut(s)	518, 787, 884, 896, 913, 1120
BstFNI	CGCG	1 cut(s)	506
BstHHI	GCGC	2 cut(s)	166, 1241
BstKTI	GATC	6 cut(s)	156, 439, 652, 957, 1054, 1373
BstMAI	GTCTC	3 cut(s)	33, 59, 706
BstMBI	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
BstMWI	GCNNNNNNNGC	8 cut(s)	93, 307, 682, 1172, 1344, 1357, 1366, 1400
BstNI	CCWGG	3 cut(s)	263, 531, 1153
BstNSI	RCATGY	2 cut(s)	689, 1351
BstSCI	CCNGG	4 cut(s)	261, 529, 1151, 1503
BstSFI	CTRYAG	1 cut(s)	390
BstUI	CGCG	1 cut(s)	506
BstV1I	GCAGC	3 cut(s)	343, 351, 376
BstV2I	GAAGAC	2 cut(s)	581, 1461
Bsu36I	CCTNAGG	1 cut(s)	1179
BsuRI	GGCC	2 cut(s)	1122, 1338
BtgI	CCRYGG	2 cut(s)	1055, 1277
BtgZI	GCGATG	1 cut(s)	158
BtsCI	GGATG	6 cut(s)	518, 787, 884, 896, 913, 1120
BtsIMutI	CAGTG	2 cut(s)	430, 1465
BveI	ACCTGC	1 cut(s)	383
Cac8I	GCNNGC	8 cut(s)	35, 168, 605, 687, 825, 1164, 1349, 1353
CciI	TCATGA	2 cut(s)	957, 1257
CfoI	GCGC	2 cut(s)	166, 1241
Cfr13I	GGNCC	1 cut(s)	667
CseI	GACGC	1 cut(s)	1383
CsiI	ACCWGGT	1 cut(s)	261
Csp6I	GTAC	3 cut(s)	405, 861, 882
CviQI	GTAC	3 cut(s)	405, 861, 882
DdeI	CTNAG	3 cut(s)	422, 810, 1179
DpnI	GATC	6 cut(s)	155, 438, 651, 956, 1053, 1372
DpnII	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
DraI	TTTAAA	1 cut(s)	1498
DraIII	CACNNNGTG	1 cut(s)	265
DriI	GACNNNNNGTC	1 cut(s)	1393
EaeI	YGGCCR	1 cut(s)	1336
Eam1104I	CTCTTC	2 cut(s)	365, 1296
Eam1105I	GACNNNNNGTC	1 cut(s)	1393
EarI	CTCTTC	2 cut(s)	365, 1296
Eco130I	CCWWGG	2 cut(s)	1055, 1277
Eco24I	GRGCYC	1 cut(s)	247
Eco31I	GGTCTC	2 cut(s)	59, 706
Eco32I	GATATC	2 cut(s)	364, 1088
Eco47I	GGWCC	1 cut(s)	667
Eco81I	CCTNAGG	1 cut(s)	1179
EcoRII	CCWGG	3 cut(s)	261, 529, 1151
EcoRV	GATATC	2 cut(s)	364, 1088
EcoT14I	CCWWGG	2 cut(s)	1055, 1277
EcoT22I	ATGCAT	2 cut(s)	691, 915
EcoT38I	GRGCYC	1 cut(s)	247
ErhI	CCWWGG	2 cut(s)	1055, 1277
FaqI	GGGAC	4 cut(s)	291, 547, 1197, 1204
FauI	CCCGC	1 cut(s)	131
FauNDI	CATATG	2 cut(s)	796, 1108
FblI	GTMKAC	1 cut(s)	1245
Fnu4HI	GCNGC	3 cut(s)	340, 357, 390
FokI	GGATG	6 cut(s)	525, 794, 891, 900, 903, 1127
FriOI	GRGCYC	1 cut(s)	247
Fsp4HI	GCNGC	3 cut(s)	340, 357, 390
FspBI	CTAG	2 cut(s)	198, 1404
GlaI	GCGC	2 cut(s)	165, 1240
GluI	GCNGC	3 cut(s)	340, 357, 390
GsaI	CCCAGC	1 cut(s)	1002
GsuI	CTGGAG	1 cut(s)	746
HaeIII	GGCC	2 cut(s)	1122, 1338
HapII	CCGG	4 cut(s)	10, 24, 176, 1505
HgaI	GACGC	1 cut(s)	1383
HhaI	GCGC	2 cut(s)	166, 1241
Hin6I	GCGC	2 cut(s)	164, 1239
HinP1I	GCGC	2 cut(s)	164, 1239
HincII	GTYRAC	2 cut(s)	1246, 1300
HindII	GTYRAC	2 cut(s)	1246, 1300
HindIII	AAGCTT	1 cut(s)	872
HinfI	GANTC	4 cut(s)	13, 41, 628, 1192
HpaII	CCGG	4 cut(s)	10, 24, 176, 1505
HphI	GGTGA	3 cut(s)	190, 233, 1389
Hpy166II	GTNNAC	3 cut(s)	224, 1246, 1300
Hpy188I	TCNGA	6 cut(s)	120, 436, 813, 942, 1387, 1489
Hpy8I	GTNNAC	3 cut(s)	224, 1246, 1300
HpyAV	CCTTC	5 cut(s)	97, 257, 632, 927, 1133
HpyCH4III	ACNGT	3 cut(s)	53, 1312, 1460
HpyCH4IV	ACGT	1 cut(s)	1219
HpyF10VI	GCNNNNNNNGC	8 cut(s)	93, 307, 682, 1172, 1344, 1357, 1366, 1400
HpyF3I	CTNAG	3 cut(s)	422, 810, 1179
HpySE526I	ACGT	1 cut(s)	1219
HspAI	GCGC	2 cut(s)	164, 1239
Kpn2I	TCCGGA	1 cut(s)	9
Kzo9I	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
LmnI	GCTCC	4 cut(s)	292, 423, 982, 1180
Lsp1109I	GCAGC	3 cut(s)	343, 351, 376
LweI	GCATC	6 cut(s)	135, 462, 772, 922, 1138, 1493
MabI	ACCWGGT	1 cut(s)	261
MaeI	CTAG	2 cut(s)	198, 1404
MaeII	ACGT	1 cut(s)	1219
MaeIII	GTNAC	2 cut(s)	1167, 1395
MalI	GATC	6 cut(s)	155, 438, 651, 956, 1053, 1372
MboI	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
MboII	GAAGA	8 cut(s)	163, 221, 382, 586, 912, 1283, 1454, 1466
MfeI	CAATTG	1 cut(s)	462
MhlI	GDGCHC	2 cut(s)	247, 261
MlsI	TGGCCA	1 cut(s)	1338
MluNI	TGGCCA	1 cut(s)	1338
MlyI	GAGTC	3 cut(s)	35, 622, 1186
Mox20I	TGGCCA	1 cut(s)	1338
Mph1103I	ATGCAT	2 cut(s)	691, 915
MroI	TCCGGA	1 cut(s)	9
MroXI	GAANNNNTTC	1 cut(s)	228
MscI	TGGCCA	1 cut(s)	1338
MslI	CAYNNNNRTG	3 cut(s)	131, 614, 776
Msp20I	TGGCCA	1 cut(s)	1338
MspA1I	CMGCKG	3 cut(s)	342, 410, 603
MspI	CCGG	4 cut(s)	10, 24, 176, 1505
MspR9I	CCNGG	4 cut(s)	263, 531, 1153, 1505
MunI	CAATTG	1 cut(s)	462
Mva1269I	GAATGC	1 cut(s)	676
MvaI	CCWGG	3 cut(s)	263, 531, 1153
MvnI	CGCG	1 cut(s)	506
MwoI	GCNNNNNNNGC	8 cut(s)	93, 307, 682, 1172, 1344, 1357, 1366, 1400
NciI	CCSGG	1 cut(s)	1505
NcoI	CCATGG	2 cut(s)	1055, 1277
NdeI	CATATG	2 cut(s)	796, 1108
NdeII	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
NlaIV	GGNNCC	3 cut(s)	288, 419, 1275
NmuCI	GTSAC	1 cut(s)	1395
NsiI	ATGCAT	2 cut(s)	691, 915
NspI	RCATGY	2 cut(s)	689, 1351
PacI	TTAATTAA	1 cut(s)	852
PaeI	GCATGC	2 cut(s)	689, 1351
PagI	TCATGA	2 cut(s)	957, 1257
PaqCI	CACCTGC	1 cut(s)	383
PctI	GAATGC	1 cut(s)	676
PdmI	GAANNNNTTC	1 cut(s)	228
PfeI	GAWTC	1 cut(s)	13
PflFI	GACNNNGTC	1 cut(s)	969
PfoI	TCCNGGA	1 cut(s)	1503
PkrI	GCNGC	3 cut(s)	341, 358, 391
PleI	GAGTC	3 cut(s)	35, 622, 1186
PpsI	GAGTC	3 cut(s)	35, 622, 1186
PshBI	ATTAAT	1 cut(s)	852
Psp6I	CCWGG	3 cut(s)	261, 529, 1151
PspFI	CCCAGC	1 cut(s)	998
PspGI	CCWGG	3 cut(s)	261, 529, 1151
PspN4I	GGNNCC	3 cut(s)	288, 419, 1275
PspPI	GGNCC	1 cut(s)	667
PstI	CTGCAG	1 cut(s)	394
PsyI	GACNNNGTC	1 cut(s)	969
PvuII	CAGCTG	2 cut(s)	410, 603
RsaI	GTAC	3 cut(s)	406, 862, 883
RsaNI	GTAC	3 cut(s)	405, 861, 882
RseI	CAYNNNNRTG	3 cut(s)	131, 614, 776
SalI	GTCGAC	1 cut(s)	1244
SatI	GCNGC	3 cut(s)	340, 357, 390
Sau3AI	GATC	6 cut(s)	153, 436, 649, 954, 1051, 1370
Sau96I	GGNCC	1 cut(s)	667
ScaI	AGTACT	1 cut(s)	862
SchI	GAGTC	3 cut(s)	35, 622, 1186
ScrFI	CCNGG	4 cut(s)	263, 531, 1153, 1505
SduI	GDGCHC	2 cut(s)	247, 261
SexAI	ACCWGGT	1 cut(s)	261
SfaNI	GCATC	6 cut(s)	135, 462, 772, 922, 1138, 1493
SfcI	CTRYAG	1 cut(s)	390
SinI	GGWCC	1 cut(s)	667
SmiMI	CAYNNNNRTG	3 cut(s)	131, 614, 776
SmlI	CTYRAG	3 cut(s)	332, 885, 1127
SmoI	CTYRAG	3 cut(s)	332, 885, 1127
SphI	GCATGC	2 cut(s)	689, 1351
SsiI	CCGC	3 cut(s)	138, 342, 674
SspMI	CTAG	2 cut(s)	198, 1404
StyD4I	CCNGG	4 cut(s)	261, 529, 1151, 1503
StyI	CCWWGG	2 cut(s)	1055, 1277
TaaI	ACNGT	3 cut(s)	53, 1312, 1460
TaiI	ACGT	1 cut(s)	1222
TaqI	TCGA	4 cut(s)	16, 62, 1245, 1364
TaqII	GACCGA	1 cut(s)	555
TatI	WGTACW	2 cut(s)	860, 881
TfiI	GAWTC	1 cut(s)	13
TscAI	CASTG	2 cut(s)	430, 1465
TseFI	GTSAC	1 cut(s)	1395
TseI	GCWGC	3 cut(s)	339, 356, 389
Tsp45I	GTSAC	1 cut(s)	1395
TspDTI	ATGAA	5 cut(s)	633, 683, 794, 974, 1246
TspGWI	ACGGA	1 cut(s)	1227
TspRI	CASTG	2 cut(s)	430, 1465
Tth111I	GACNNNGTC	1 cut(s)	969
VpaK11BI	GGWCC	1 cut(s)	667
VspI	ATTAAT	1 cut(s)	852
XapI	RAATTY	3 cut(s)	18, 158, 1323
XceI	RCATGY	2 cut(s)	689, 1351
XcmI	CCANNNNNNNNNTGG	3 cut(s)	475, 506, 743
XmiI	GTMKAC	1 cut(s)	1245
XmnI	GAANNNNTTC	1 cut(s)	228
XspI	CTAG	2 cut(s)	198, 1404
ZrmI	AGTACT	1 cut(s)	862
Zsp2I	ATGCAT	2 cut(s)	691, 915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.