AT2G30300

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001224
Physical Location & Seq
Forward (+)
12919401 .. 12921431
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30300.1

Sequence Viewer

Length: 1503 bp
ATGTCTCGGAAGATTCTCCCGTGGTTGAGTCTAGTCGCTATTCTCTGGCTTCAATCTTTCAATGGCACCAACTTGAGTTTCCCAGCTTACTCTTCACAGCTTAAAGAGCTTCTCAAAATCTCCCAGTTTAAGCTAAATTATCTTTCTTTCGCCTCCGACGCTGGAAAAGTCCTAGGTTTTATCTCCGGTATCGCCGCCGTTTATCTTCCTCTCCCTCTTGTCCTTCTCGCCGGAGGTTCATTAGGGTTCGCCGGCTACGGTCTCCAGTATCTCTCCATCATCAAGAAGATTATCTGTTGGATCAACACAGCTTGTTACATCGTCGCAATCAATAGTTTTCCGGTGAACCGTCAAGTCGCCGTGGGGATCACGGCAAGTTATCAAGGTTTGAGTGGGAAAATCTACACCGACATGGTCCACTCTTTTTTTCACACGTCTCAACGTGAAGAAGCTTCGGGTTATCTCTTGCTCAACTCTCTTGTTCCATTGGTCGCTTGTCTCGTGACGGCTCCTATGTTGATGAGACACGGAGGAGACAAAACGATGTCGTATTCTAAGGATGTTAAAGTTGGTTTTATCGTTTTGTTCGTCTTGACTATTGCTACGGGGATTTATGCGGTGGCTACGAGTCTTGTCTCGGTTCCTGCGGTTTTAGTACTTGTAGGCATTGCTCTGTTTCTTCTTGCTCCTCTTGCTATACCAATTGGAGTTGGATTTAAAGAGCTTATGTCCTCTAGAAAAACTCAACAAAAGGTGCACGATCTTGAAGCTCCTGTCGATAAATTTTATTTCGTAGAAGAAGATCACACGAAAGAAGAAGAAGAGTTTGAGAAGGCAATAATTGGAGTTAAAGAAGAGGTCGAGTGGACACAACTATGGAAGAAACTCGATTTTTGGATATACTTTGGACTCTATTTGTTCGGTCCAACGGTCGGGCTAGTGTTTACGAACAATCTTGGTCAGATCGCTGAGTCTCGTGGCTCCACCGCCACCAGTTCCTTGGTCGCTCTCTCGTCGTCGTTCGGGTTTTTCGGCCGCTTGTTACCATCATTACTCGACTACTTCTTTTCTAGGAACAAATACATGCCATCAAGTCCGGTTTCTATGGCGGGTTCACTGGTAGCAATGGTAGCTTCGTTTCTCCTCCTCCTTATTGACTCCGACATTGCTCTCTACATCGGCACGGCGATGATTGGCATCTTCTCCGGAGCCTTGACTTCACTCTCTGTCACAATGACGGCAGAGCTTTTTGGGACGAAGCATTTTGGAGTTAACCACAACATTGTTGTCGGAAGTATTCCCCTTGGATCTTTTTCTTTCGGTTTGTTAGCCGCTAAAATTTACCGCGACGGAGCTGCTCTTTACGGAGATGATGGCAAGTGTTTTGGGATGCATTGCTTTCAAACCACTTTGGTATTTTGGGGAATGCTTTGTTCGATCGCCGCACTTCTCGCCGCCGTTTTGTATATACGCAACCGCAAGTTCTATTCGCAGAAGCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

500

Amino Acids

54.59

Weight (kDa)

8.79

Isoelectric Point (pI)

33.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 7 - 97 3e-29 Nodulin-like
Nodulin-like PF06813 98 - 235 2.4e-25 Nodulin-like
NFD4_C PF23262 282 - 497 2.6e-74 NFD4, C-terminal domain
MFS_1 PF07690 369 - 494 6.1e-06 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 1 cut(s) 1347
AccIII TCCGGA 1 cut(s) 1205
AclWI GGATC 3 cut(s) 308, 374, 1315
AcoI YGGCCR 1 cut(s) 1033
AcsI RAATTY 2 cut(s) 782, 1338
AfaI GTAC 1 cut(s) 657
AfiI CCNNNNNNNGG 1 cut(s) 932
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 4 cut(s) 53, 61, 767, 1403
AjiI CACGTC 1 cut(s) 435
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 8 cut(s) 9, 266, 441, 503, 517, 528, 640, 978
Alw44I GTGCAC 1 cut(s) 755
AlwI GGATC 3 cut(s) 308, 374, 1315
Aor13HI TCCGGA 1 cut(s) 1205
AoxI GGCC 1 cut(s) 1033
ApaLI GTGCAC 1 cut(s) 755
ApeKI GCWGC 1 cut(s) 1355
ApoI RAATTY 2 cut(s) 782, 1338
AspA2I CCTAGG 1 cut(s) 172
AspS9I GGNCC 2 cut(s) 415, 923
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 2 cut(s) 415, 923
AvrII CCTAGG 1 cut(s) 172
BaeGI GKGCMC 1 cut(s) 759
BanI GGYRCC 1 cut(s) 65
BauI CACGAG 2 cut(s) 500, 975
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 1 cut(s) 1342
BccI CCATC 4 cut(s) 284, 1054, 1096, 1367
BceAI ACGGC 8 cut(s) 182, 344, 387, 522, 1200, 1254, 1442, 1483
BcgI CGANNNNNNTGC 2 cut(s) 1337, 1371
BcoDI GTCTC 8 cut(s) 9, 266, 441, 503, 517, 528, 640, 978
BfaI CTAG 5 cut(s) 32, 173, 735, 938, 1071
BisI GCNGC 6 cut(s) 195, 1036, 1332, 1356, 1443, 1455
BlnI CCTAGG 1 cut(s) 172
BlsI GCNGC 6 cut(s) 196, 1037, 1333, 1357, 1444, 1456
BmcAI AGTACT 1 cut(s) 657
Bme18I GGWCC 2 cut(s) 415, 923
BmgBI CACGTC 1 cut(s) 435
BmgT120I GGNCC 2 cut(s) 415, 923
BmiI GGNNCC 5 cut(s) 67, 510, 642, 982, 1210
BmrI ACTGGG 1 cut(s) 118
BmsI GCATC 2 cut(s) 1206, 1380
BmuI ACTGGG 1 cut(s) 118
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 94
BsaBI GATNNNNATC 1 cut(s) 1196
BsaI GGTCTC 1 cut(s) 266
BsaJI CCNNGG 5 cut(s) 20, 172, 360, 999, 1303
BsaWI WCCGGW 4 cut(s) 185, 340, 1096, 1205
Bsc4I CCNNNNNNNGG 1 cut(s) 932
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 4 cut(s) 124, 265, 993, 1122
Bse3DI GCAATG 4 cut(s) 666, 1131, 1164, 1393
Bse8I GATNNNNATC 1 cut(s) 1196
BseAI TCCGGA 1 cut(s) 1205
BseDI CCNNGG 5 cut(s) 20, 172, 360, 999, 1303
BseGI GGATG 2 cut(s) 565, 1395
BseJI GATNNNNATC 1 cut(s) 1196
BseLI CCNNNNNNNGG 1 cut(s) 932
BseMI GCAATG 4 cut(s) 666, 1131, 1164, 1393
BseMII CTCAG 1 cut(s) 960
BseNI ACTGG 4 cut(s) 124, 265, 993, 1122
BseRI GAGGAG 4 cut(s) 546, 678, 1133, 1136
BseSI GKGCMC 1 cut(s) 759
BseX3I CGGCCG 1 cut(s) 1033
BseXI GCAGC 1 cut(s) 1342
BseYI CCCAGC 1 cut(s) 82
Bsh1236I CGCG 1 cut(s) 1347
Bsh1285I CGRYCG 3 cut(s) 933, 1036, 1440
BshFI GGCC 1 cut(s) 1035
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 3 cut(s) 933, 1036, 1440
BsiHKAI GWGCWC 1 cut(s) 759
BsiSI CCGG 6 cut(s) 186, 231, 252, 341, 1097, 1206
BslFI GGGAC 1 cut(s) 1267
BslI CCNNNNNNNGG 1 cut(s) 932
BsmAI GTCTC 8 cut(s) 9, 266, 441, 503, 517, 528, 640, 978
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 1267
BsmI GAATGC 1 cut(s) 1431
BsnI GGCC 1 cut(s) 1035
Bso31I GGTCTC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 759
Bsp13I TCCGGA 1 cut(s) 1205
Bsp143I GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
BspANI GGCC 1 cut(s) 1035
BspCNI CTCAG 1 cut(s) 961
BspEI TCCGGA 1 cut(s) 1205
BspFNI CGCG 1 cut(s) 1347
BspLI GGNNCC 5 cut(s) 67, 510, 642, 982, 1210
BspPI GGATC 3 cut(s) 308, 374, 1315
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 266
BsrDI GCAATG 4 cut(s) 666, 1131, 1164, 1393
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 4 cut(s) 124, 265, 993, 1122
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 5 cut(s) 20, 172, 360, 999, 1303
BssMI GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
BssSI CACGAG 2 cut(s) 500, 975
BssT1I CCWWGG 3 cut(s) 172, 999, 1303
Bst2BI CACGAG 2 cut(s) 500, 975
Bst4CI ACNGT 3 cut(s) 260, 350, 931
Bst6I CTCTTC 3 cut(s) 97, 816, 849
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 2 cut(s) 555, 969
BstDSI CCRYGG 2 cut(s) 20, 360
BstF5I GGATG 2 cut(s) 565, 1395
BstFNI CGCG 1 cut(s) 1347
BstKTI GATC 7 cut(s) 303, 369, 763, 805, 966, 1310, 1440
BstMAI GTCTC 8 cut(s) 9, 266, 441, 503, 517, 528, 640, 978
BstMBI GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
BstMCI CGRYCG 3 cut(s) 933, 1036, 1440
BstMWI GCNNNNNNNGC 5 cut(s) 106, 158, 692, 1130, 1451
BstNSI RCATGY 1 cut(s) 1087
BstSLI GKGCMC 1 cut(s) 759
BstUI CGCG 1 cut(s) 1347
BstV1I GCAGC 1 cut(s) 1342
BstX2I RGATCY 1 cut(s) 1307
BstXI CCANNNNNNTGG 1 cut(s) 1000
BstYI RGATCY 1 cut(s) 1307
BstZI CGGCCG 1 cut(s) 1033
BsuRI GGCC 1 cut(s) 1035
BtgI CCRYGG 2 cut(s) 20, 360
BtgZI GCGATG 1 cut(s) 1202
BtrI CACGTC 1 cut(s) 435
BtsCI GGATG 2 cut(s) 565, 1395
BtsIMutI CAGTG 1 cut(s) 1115
Cac8I GCNNGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 415, 923
CseI GACGC 1 cut(s) 167
Csp6I GTAC 1 cut(s) 656
CviAII CATG 2 cut(s) 412, 1084
CviQI GTAC 1 cut(s) 656
DdeI CTNAG 2 cut(s) 555, 969
DpnI GATC 7 cut(s) 302, 368, 762, 804, 965, 1309, 1439
DpnII GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
DraI TTTAAA 1 cut(s) 718
EaeI YGGCCR 1 cut(s) 1033
EagI CGGCCG 1 cut(s) 1033
Eam1104I CTCTTC 3 cut(s) 97, 816, 849
EarI CTCTTC 3 cut(s) 97, 816, 849
EclXI CGGCCG 1 cut(s) 1033
Eco130I CCWWGG 3 cut(s) 172, 999, 1303
Eco31I GGTCTC 1 cut(s) 266
Eco47I GGWCC 2 cut(s) 415, 923
Eco52I CGGCCG 1 cut(s) 1033
EcoT14I CCWWGG 3 cut(s) 172, 999, 1303
EcoT22I ATGCAT 1 cut(s) 1395
ErhI CCWWGG 3 cut(s) 172, 999, 1303
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 2 cut(s) 415, 1087
FaqI GGGAC 1 cut(s) 1267
FatI CATG 2 cut(s) 411, 1083
FauI CCCGC 1 cut(s) 1102
Fnu4HI GCNGC 6 cut(s) 195, 1036, 1332, 1356, 1443, 1455
FokI GGATG 2 cut(s) 572, 1402
Fsp4HI GCNGC 6 cut(s) 195, 1036, 1332, 1356, 1443, 1455
FspBI CTAG 5 cut(s) 32, 173, 735, 938, 1071
GluI GCNGC 6 cut(s) 195, 1036, 1332, 1356, 1443, 1455
GsaI CCCAGC 1 cut(s) 86
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 1 cut(s) 1035
HapII CCGG 6 cut(s) 186, 231, 252, 341, 1097, 1206
HgaI GACGC 1 cut(s) 167
Hin1II CATG 2 cut(s) 415, 1087
HincII GTYRAC 1 cut(s) 1273
HindII GTYRAC 1 cut(s) 1273
HindIII AAGCTT 1 cut(s) 450
HinfI GANTC 6 cut(s) 13, 28, 628, 909, 971, 1157
HpaI GTTAAC 1 cut(s) 1273
HpaII CCGG 6 cut(s) 186, 231, 252, 341, 1097, 1206
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 7 cut(s) 346, 418, 757, 867, 945, 1115, 1273
Hpy188I TCNGA 5 cut(s) 9, 157, 963, 1162, 1292
Hpy188III TCNNGA 6 cut(s) 283, 502, 592, 735, 764, 1206
Hpy8I GTNNAC 7 cut(s) 346, 418, 757, 867, 945, 1115, 1273
Hpy99I CGWCG 5 cut(s) 161, 326, 1018, 1021, 1352
HpyAV CCTTC 2 cut(s) 233, 826
HpyCH4III ACNGT 3 cut(s) 260, 350, 931
HpyCH4IV ACGT 2 cut(s) 434, 442
HpyCH4V TGCA 2 cut(s) 757, 1393
HpyF10VI GCNNNNNNNGC 5 cut(s) 106, 158, 692, 1130, 1451
HpyF3I CTNAG 2 cut(s) 555, 969
HpySE526I ACGT 2 cut(s) 434, 442
Hsp92II CATG 2 cut(s) 415, 1087
Kpn2I TCCGGA 1 cut(s) 1205
KroI GCCGGC 1 cut(s) 251
KroNI GCCGGC 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 1273
Kzo9I GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
LmnI GCTCC 6 cut(s) 514, 691, 775, 986, 1208, 1352
Lsp1109I GCAGC 1 cut(s) 1342
LweI GCATC 2 cut(s) 1206, 1380
MaeI CTAG 5 cut(s) 32, 173, 735, 938, 1071
MaeII ACGT 2 cut(s) 434, 442
MaeIII GTNAC 4 cut(s) 314, 502, 1041, 1228
MalI GATC 7 cut(s) 302, 368, 762, 804, 965, 1309, 1439
MboI GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 1 cut(s) 1307
MhlI GDGCHC 1 cut(s) 759
MluCI AATT 5 cut(s) 136, 702, 782, 840, 1338
MlyI GAGTC 5 cut(s) 37, 637, 903, 980, 1151
MmeI TCCRAC 6 cut(s) 180, 278, 691, 950, 1185, 1270
Mph1103I ATGCAT 1 cut(s) 1395
MroI TCCGGA 1 cut(s) 1205
MroNI GCCGGC 1 cut(s) 251
MseI TTAA 6 cut(s) 102, 129, 564, 717, 849, 1272
MslI CAYNNNNRTG 3 cut(s) 410, 874, 1187
MspI CCGG 6 cut(s) 186, 231, 252, 341, 1097, 1206
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 1431
MvnI CGCG 1 cut(s) 1347
MwoI GCNNNNNNNGC 5 cut(s) 106, 158, 692, 1130, 1451
NaeI GCCGGC 1 cut(s) 253
NdeII GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
NgoMIV GCCGGC 1 cut(s) 251
NlaIII CATG 2 cut(s) 415, 1087
NlaIV GGNNCC 5 cut(s) 67, 510, 642, 982, 1210
NmuCI GTSAC 2 cut(s) 502, 1228
NsiI ATGCAT 1 cut(s) 1395
NspI RCATGY 1 cut(s) 1087
PcsI WCGNNNNNNNCGW 3 cut(s) 498, 585, 1496
PctI GAATGC 1 cut(s) 1431
PdiI GCCGGC 1 cut(s) 253
PfeI GAWTC 1 cut(s) 13
PflFI GACNNNGTC 1 cut(s) 413
PkrI GCNGC 6 cut(s) 196, 1037, 1333, 1357, 1444, 1456
Ple19I CGATCG 1 cut(s) 1440
PleI GAGTC 5 cut(s) 36, 636, 903, 979, 1151
PpsI GAGTC 5 cut(s) 36, 636, 903, 979, 1151
PspFI CCCAGC 1 cut(s) 82
PspN4I GGNNCC 5 cut(s) 67, 510, 642, 982, 1210
PspPI GGNCC 2 cut(s) 415, 923
PsuI RGATCY 1 cut(s) 1307
PsyI GACNNNGTC 1 cut(s) 413
PvuI CGATCG 1 cut(s) 1440
RsaI GTAC 1 cut(s) 657
RsaNI GTAC 1 cut(s) 656
RseI CAYNNNNRTG 3 cut(s) 410, 874, 1187
SaqAI TTAA 6 cut(s) 102, 129, 564, 717, 849, 1272
SatI GCNGC 6 cut(s) 195, 1036, 1332, 1356, 1443, 1455
Sau3AI GATC 7 cut(s) 300, 366, 760, 802, 963, 1307, 1437
Sau96I GGNCC 2 cut(s) 415, 923
ScaI AGTACT 1 cut(s) 657
SchI GAGTC 5 cut(s) 37, 637, 903, 980, 1151
SduI GDGCHC 1 cut(s) 759
SfaNI GCATC 2 cut(s) 1206, 1380
SinI GGWCC 2 cut(s) 415, 923
SmiMI CAYNNNNRTG 3 cut(s) 410, 874, 1187
SmlI CTYRAG 1 cut(s) 73
SmoI CTYRAG 1 cut(s) 73
Sse9I AATT 5 cut(s) 136, 702, 782, 840, 1338
SspMI CTAG 5 cut(s) 32, 173, 735, 938, 1071
StyI CCWWGG 3 cut(s) 172, 999, 1303
TaaI ACNGT 3 cut(s) 260, 350, 931
TaiI ACGT 2 cut(s) 437, 445
TaqI TCGA 5 cut(s) 777, 861, 888, 1056, 1436
TaqII GACCGA 1 cut(s) 911
TasI AATT 5 cut(s) 136, 702, 782, 840, 1338
TatI WGTACW 1 cut(s) 655
TauI GCSGC 5 cut(s) 197, 1038, 1334, 1445, 1457
TfiI GAWTC 1 cut(s) 13
Tru1I TTAA 6 cut(s) 102, 129, 564, 717, 849, 1272
Tru9I TTAA 6 cut(s) 102, 129, 564, 717, 849, 1272
TscAI CASTG 1 cut(s) 1122
TseFI GTSAC 2 cut(s) 502, 1228
TseI GCWGC 1 cut(s) 1355
Tsp45I GTSAC 2 cut(s) 502, 1228
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 3 cut(s) 543, 1365, 1380
TspRI CASTG 1 cut(s) 1122
Tth111I GACNNNGTC 1 cut(s) 413
VneI GTGCAC 1 cut(s) 755
VpaK11BI GGWCC 2 cut(s) 415, 923
XapI RAATTY 2 cut(s) 782, 1338
XbaI TCTAGA 1 cut(s) 734
XceI RCATGY 1 cut(s) 1087
XcmI CCANNNNNNNNNTGG 1 cut(s) 997
XmaJI CCTAGG 1 cut(s) 172
XspI CTAG 5 cut(s) 32, 173, 735, 938, 1071
ZrmI AGTACT 1 cut(s) 657
Zsp2I ATGCAT 1 cut(s) 1395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.