Rh5AG472700

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001224
Physical Location & Seq
Forward (+)
81199109 .. 81200856
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG472700.1

Sequence Viewer

Length: 1608 bp
ATGTCTTCCTCATCAAAAGCTTTCCAATGGTTAAGCCTAGTGGCCATCATATGGCTTCAGTCCATAAATGGAACAAACACCACCTTCCCTGCTTACTCATCCCAACTCAAGAACACTCTATCCTTGTCCCAACTACAAATCAACAATCTCGTCTTTGCCTCCGACGCAGGGAAGCTCTTTGGTTGGTTTTCCGGCATTGCTGCTATTTACCTTCCCCTTTGGCGAGTCCTCATGGTCGGCTCACTTCTCGGGTTGATTGGCTACGGCGTCCAATTTCTATTCGTAACAGACAAAATCTCATCTCCAGCATATTGGCAAATATTCTTGCTCACCATGGTGGCCGGAAATAGCGTCTGTTGGATCAACACCGTTTGCTATGTTGTTAGTATTCGAAACTTTCCGTTTCACCAACAGGTTGCTGTGGGACTAACAACAAGTTATCAAGGATTGAGTGCAAAAATCTACACAGATATTGTTAGTACTGCTTTTTCTTCTTCACCTCATAAAAGAGCACAGGCCTATCTTCTTCTAAACTCTCTCCTGCCTTTACTAGTCTGTGTTGTAGTGGCACCATTGGTGAGAAACATTGATGTGGGAACAGGGGAGCCGAGAAACATGGCAGCGGGGTTCAATTTGATGTTTGTGATAACCATTGCCACCGGAATCTATGCCGTCATCACCAGTTTAGGATCAGTGATCGGCTTAGACAGAATGATAGGTACCGGAGTGTTCCTACTGATCCCCTTGGTAATCCCAATTGTAGTAAAAATCAGAGAAAAATTATCAAGAAGATGGAGCCTAGACAGAGAGAACAGAGTGTACAATGTCAGCATAGATGATCAGAATGGCCTGGAGAGCATAGAAAGTGGTGTAGTTAATAAACAGGAAGAAGAAGAGGAACATAGTACAGTGCAGGTTCATGATGAGGTTGGTGAGATACAGGTTAATCATGGGATTGGTGTTAGAGAGGAGATTGGAGTGAAGTTGATGGTGCAAAGGATAGATTTCTGGTTATATTTCCTAGTGTATTTTTTCAGTGCAACAATTGGGCTTGTGTTCTTGAACACCTTGGGACAGATAGCGGAGTCAAGAGGCTCATCTATGACTTCTTCTCTGGTTTCTTTATCTTCTTCCTTCGGGTTCTTTGGCCGTCTCATGCCTTCACTTCTGGACTATTTCTTCTCAAGAAGCAAGTATATGATTTCAAGGCCTGCATTCCTAATGGCATTAGCAGCACCAATGGCAGGAGTTTTTTTTTTGCTTCTCAACCCAGCTAACGTCTCTCTTATCATCAGTACCGCCATTATAGGAGTGTGCACTGGAGCAATTACTTCTATTGCAGTGTCCATAACCACAGAGTTATTCGGGACCAAGAAATTCTCAATAAATCACAATGTGGTGGTTGCGAATATCTCAGTCGGGTCGTTTCTTTTTGGCTACTCATCTGCTCTTCTTTATCGCAAGGAAGGAGATGAAGATGGTAGCTGCTTGGGAATGGCTTGTTACCGAAGCACCTTCATTATCTGGGGTAGTCTCTGTACGTTAGGGACTGTCCTAGCTTTAACGCTATATGCAAGAACAAGAAAGTTCTACTCCAAAAGATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nodulin-like

Protein Analysis

535

Amino Acids

58.73

Weight (kDa)

8.93

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 9 - 256 2.9e-72 Nodulin-like
NFD4_C PF23262 319 - 532 1.5e-102 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 904
Acc65I GGTACC 1 cut(s) 719
AccB1I GGYRCC 2 cut(s) 568, 719
AccB7I CCANNNNNTGG 1 cut(s) 51
AciI CCGC 3 cut(s) 623, 1082, 1299
AclWI GGATC 3 cut(s) 368, 697, 733
AcoI YGGCCR 3 cut(s) 42, 339, 1147
AcsI RAATTY 1 cut(s) 1376
AcuI CTGAAG 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 1396
AfaI GTAC 6 cut(s) 481, 721, 821, 907, 1297, 1540
AfiI CCNNNNNNNGG 3 cut(s) 51, 168, 1244
AgsI TTSAA 3 cut(s) 631, 1063, 1206
AhlI ACTAGT 1 cut(s) 550
AjnI CCWGG 1 cut(s) 849
AleI CACNNNNGTG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 5 cut(s) 20, 175, 1274, 1485, 1559
AluI AGCT 5 cut(s) 20, 175, 1274, 1485, 1559
Alw21I GWGCWC 2 cut(s) 514, 1319
Alw26I GTCTC 3 cut(s) 1157, 1285, 1538
Alw44I GTGCAC 1 cut(s) 1315
AlwI GGATC 3 cut(s) 368, 697, 733
Ama87I CYCGRG 1 cut(s) 248
AoxI GGCC 6 cut(s) 42, 339, 516, 847, 1147, 1208
ApaLI GTGCAC 1 cut(s) 1315
ApeKI GCWGC 4 cut(s) 200, 620, 1232, 1485
ApoI RAATTY 1 cut(s) 1376
Asp718I GGTACC 1 cut(s) 719
AspS9I GGNCC 1 cut(s) 1368
AsuHPI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 944
AsuII TTCGAA 1 cut(s) 391
AvaI CYCGRG 1 cut(s) 248
AvaII GGWCC 1 cut(s) 1368
BaeGI GKGCMC 1 cut(s) 1319
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 2 cut(s) 568, 719
Bbv12I GWGCWC 2 cut(s) 514, 1319
BbvI GCAGC 4 cut(s) 187, 632, 1244, 1472
BccI CCATC 4 cut(s) 53, 786, 982, 1472
BceAI ACGGC 3 cut(s) 280, 656, 1134
BciT130I CCWGG 1 cut(s) 851
BclI TGATCA 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 1157, 1285, 1538
BcuI ACTAGT 1 cut(s) 550
BfaI CTAG 5 cut(s) 38, 551, 800, 1022, 1556
BfuAI ACCTGC 1 cut(s) 904
BisI GCNGC 4 cut(s) 201, 621, 1233, 1486
BlsI GCNGC 4 cut(s) 202, 622, 1234, 1487
BmcAI AGTACT 1 cut(s) 481
Bme1390I CCNGG 1 cut(s) 851
Bme18I GGWCC 1 cut(s) 1368
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 1368
BmiI GGNNCC 5 cut(s) 570, 606, 721, 797, 1369
BmrFI CCNGG 1 cut(s) 851
BpmI CTGGAG 3 cut(s) 288, 872, 1341
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 2 cut(s) 92, 1168
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 3 cut(s) 333, 744, 1068
BsaWI WCCGGW 2 cut(s) 659, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 168, 1244
Bse1I ACTGG 2 cut(s) 681, 1324
Bse3DI GCAATG 2 cut(s) 195, 651
BseBI CCWGG 1 cut(s) 851
BseDI CCNNGG 3 cut(s) 333, 744, 1068
BseGI GGATG 1 cut(s) 98
BseLI CCNNNNNNNGG 3 cut(s) 51, 168, 1244
BseMI GCAATG 2 cut(s) 195, 651
BseMII CTCAG 1 cut(s) 1428
BseNI ACTGG 2 cut(s) 681, 1324
BseRI GAGGAG 1 cut(s) 983
BseSI GKGCMC 1 cut(s) 1319
BseXI GCAGC 4 cut(s) 187, 632, 1244, 1472
BseYI CCCAGC 1 cut(s) 1270
BsgI GTGCAG 1 cut(s) 932
BshFI GGCC 6 cut(s) 44, 341, 518, 849, 1149, 1210
BshNI GGYRCC 2 cut(s) 568, 719
BsiHKAI GWGCWC 2 cut(s) 514, 1319
BsiHKCI CYCGRG 1 cut(s) 248
BsiSI CCGG 4 cut(s) 192, 342, 660, 723
BslFI GGGAC 5 cut(s) 112, 438, 1086, 1381, 1561
BslI CCNNNNNNNGG 3 cut(s) 51, 168, 1244
BsmAI GTCTC 3 cut(s) 1157, 1285, 1538
BsmBI CGTCTC 2 cut(s) 1157, 1285
BsmFI GGGAC 5 cut(s) 112, 438, 1086, 1381, 1561
BsmI GAATGC 1 cut(s) 1214
BsnI GGCC 6 cut(s) 44, 341, 518, 849, 1149, 1210
BsoBI CYCGRG 1 cut(s) 248
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 2 cut(s) 514, 1319
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 3 cut(s) 623, 1082, 1299
BspANI GGCC 6 cut(s) 44, 341, 518, 849, 1149, 1210
BspCNI CTCAG 1 cut(s) 1427
BspHI TCATGA 1 cut(s) 919
BspLI GGNNCC 5 cut(s) 570, 606, 721, 797, 1369
BspMI ACCTGC 1 cut(s) 904
BspPI GGATC 3 cut(s) 368, 697, 733
BspQI GCTCTTC 1 cut(s) 1455
BspT104I TTCGAA 1 cut(s) 391
BspT107I GGYRCC 2 cut(s) 568, 719
BsrDI GCAATG 2 cut(s) 195, 651
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 2 cut(s) 681, 1324
BssECI CCNNGG 3 cut(s) 333, 744, 1068
BssMI GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 3 cut(s) 333, 744, 1068
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 3 cut(s) 370, 910, 1552
Bst6I CTCTTC 2 cut(s) 888, 1455
BstACI GRCGYC 1 cut(s) 267
BstAUI TGTACA 1 cut(s) 819
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 1 cut(s) 1212
BstDEI CTNAG 2 cut(s) 703, 1414
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 6 cut(s) 363, 692, 699, 741, 841, 1604
BstMAI GTCTC 3 cut(s) 1157, 1285, 1538
BstMBI GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
BstMWI GCNNNNNNNGC 4 cut(s) 164, 855, 1232, 1241
BstNI CCWGG 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 849
BstSLI GKGCMC 1 cut(s) 1319
BstV1I GCAGC 4 cut(s) 187, 632, 1244, 1472
BstXI CCANNNNNNTGG 1 cut(s) 312
BsuRI GGCC 6 cut(s) 44, 341, 518, 849, 1149, 1210
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 1347
BtsIMutI CAGTG 5 cut(s) 699, 915, 1042, 1317, 1347
BveI ACCTGC 1 cut(s) 904
Cac8I GCNNGC 1 cut(s) 1212
CciI TCATGA 1 cut(s) 919
Cfr13I GGNCC 1 cut(s) 1368
CseI GACGC 3 cut(s) 173, 256, 340
Csp6I GTAC 6 cut(s) 480, 720, 820, 906, 1296, 1539
CviAII CATG 6 cut(s) 232, 334, 616, 920, 950, 1156
CviQI GTAC 6 cut(s) 480, 720, 820, 906, 1296, 1539
DdeI CTNAG 2 cut(s) 703, 1414
DpnI GATC 6 cut(s) 362, 691, 698, 740, 840, 1603
DpnII GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
DraIII CACNNNGTG 1 cut(s) 1396
EaeI YGGCCR 3 cut(s) 42, 339, 1147
Eam1104I CTCTTC 2 cut(s) 888, 1455
EarI CTCTTC 2 cut(s) 888, 1455
Eco130I CCWWGG 3 cut(s) 333, 744, 1068
Eco147I AGGCCT 2 cut(s) 518, 1210
Eco47I GGWCC 1 cut(s) 1368
Eco57I CTGAAG 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 3 cut(s) 333, 744, 1068
ErhI CCWWGG 3 cut(s) 333, 744, 1068
Esp3I CGTCTC 2 cut(s) 1157, 1285
FaeI CATG 6 cut(s) 235, 337, 619, 923, 953, 1159
FaqI GGGAC 5 cut(s) 112, 438, 1086, 1381, 1561
FatI CATG 6 cut(s) 231, 333, 615, 919, 949, 1155
FauI CCCGC 1 cut(s) 616
FauNDI CATATG 1 cut(s) 50
FbaI TGATCA 1 cut(s) 838
Fnu4HI GCNGC 4 cut(s) 201, 621, 1233, 1486
FokI GGATG 1 cut(s) 85
Fsp4HI GCNGC 4 cut(s) 201, 621, 1233, 1486
FspBI CTAG 5 cut(s) 38, 551, 800, 1022, 1556
GluI GCNGC 4 cut(s) 201, 621, 1233, 1486
GsaI CCCAGC 1 cut(s) 1274
GsuI CTGGAG 3 cut(s) 288, 872, 1341
HaeIII GGCC 6 cut(s) 44, 341, 518, 849, 1149, 1210
HapII CCGG 4 cut(s) 192, 342, 660, 723
HgaI GACGC 3 cut(s) 173, 256, 340
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 235, 337, 619, 923, 953, 1159
HindIII AAGCTT 1 cut(s) 18
HinfI GANTC 3 cut(s) 225, 663, 1085
HpaII CCGG 4 cut(s) 192, 342, 660, 723
HphI GGTGA 6 cut(s) 322, 398, 489, 589, 670, 944
Hpy166II GTNNAC 2 cut(s) 820, 1317
Hpy188I TCNGA 3 cut(s) 163, 773, 843
Hpy188III TCNNGA 8 cut(s) 109, 786, 920, 1060, 1089, 1169, 1185, 1366
Hpy8I GTNNAC 2 cut(s) 820, 1317
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 6 cut(s) 94, 221, 1144, 1170, 1460, 1525
HpyCH4III ACNGT 3 cut(s) 370, 910, 1552
HpyCH4IV ACGT 2 cut(s) 1278, 1541
HpyCH4V TGCA 8 cut(s) 455, 913, 994, 1040, 1214, 1317, 1340, 1574
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 855, 1232, 1241
HpyF3I CTNAG 2 cut(s) 703, 1414
HpySE526I ACGT 2 cut(s) 1278, 1541
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 235, 337, 619, 923, 953, 1159
KpnI GGTACC 1 cut(s) 723
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
LguI GCTCTTC 1 cut(s) 1455
LmnI GCTCC 3 cut(s) 604, 795, 1322
Lsp1109I GCAGC 4 cut(s) 187, 632, 1244, 1472
MaeI CTAG 5 cut(s) 38, 551, 800, 1022, 1556
MaeII ACGT 2 cut(s) 1278, 1541
MaeIII GTNAC 2 cut(s) 283, 1502
MalI GATC 6 cut(s) 362, 691, 698, 740, 840, 1603
MboI GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
MfeI CAATTG 2 cut(s) 756, 1044
MhlI GDGCHC 2 cut(s) 514, 1319
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 7 cut(s) 272, 631, 756, 779, 1044, 1326, 1376
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 234, 1094
MmeI TCCRAC 2 cut(s) 186, 338
MnlI CCTC 8 cut(s) 19, 169, 239, 510, 889, 919, 961, 1085
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 4 cut(s) 32, 876, 945, 1562
MslI CAYNNNNRTG 2 cut(s) 335, 590
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 4 cut(s) 192, 342, 660, 723
MspR9I CCNGG 1 cut(s) 851
MunI CAATTG 2 cut(s) 756, 1044
Mva1269I GAATGC 1 cut(s) 1214
MvaI CCWGG 1 cut(s) 851
MwoI GCNNNNNNNGC 4 cut(s) 164, 855, 1232, 1241
NcoI CCATGG 1 cut(s) 333
NdeI CATATG 1 cut(s) 50
NdeII GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
NlaIII CATG 6 cut(s) 235, 337, 619, 923, 953, 1159
NlaIV GGNNCC 5 cut(s) 570, 606, 721, 797, 1369
NmeAIII GCCGAG 1 cut(s) 633
NspV TTCGAA 1 cut(s) 391
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 919
PceI AGGCCT 2 cut(s) 518, 1210
PciSI GCTCTTC 1 cut(s) 1455
PctI GAATGC 1 cut(s) 1214
PfeI GAWTC 1 cut(s) 663
PflMI CCANNNNNTGG 1 cut(s) 51
PkrI GCNGC 4 cut(s) 202, 622, 1234, 1487
PleI GAGTC 2 cut(s) 233, 1093
PpsI GAGTC 2 cut(s) 233, 1093
Psp6I CCWGG 1 cut(s) 849
PspFI CCCAGC 1 cut(s) 1270
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 5 cut(s) 570, 606, 721, 797, 1369
PspPI GGNCC 1 cut(s) 1368
PsrI GAACNNNNNNTAC 2 cut(s) 803, 835
RsaI GTAC 6 cut(s) 481, 721, 821, 907, 1297, 1540
RsaNI GTAC 6 cut(s) 480, 720, 820, 906, 1296, 1539
RseI CAYNNNNRTG 2 cut(s) 335, 590
SapI GCTCTTC 1 cut(s) 1455
SaqAI TTAA 4 cut(s) 32, 876, 945, 1562
SatI GCNGC 4 cut(s) 201, 621, 1233, 1486
Sau3AI GATC 6 cut(s) 360, 689, 696, 738, 838, 1601
Sau96I GGNCC 1 cut(s) 1368
ScaI AGTACT 1 cut(s) 481
SchI GAGTC 2 cut(s) 234, 1094
ScrFI CCNGG 1 cut(s) 851
SduI GDGCHC 2 cut(s) 514, 1319
SfuI TTCGAA 1 cut(s) 391
SinI GGWCC 1 cut(s) 1368
SmiMI CAYNNNNRTG 2 cut(s) 335, 590
SmlI CTYRAG 2 cut(s) 107, 1183
SmoI CTYRAG 2 cut(s) 107, 1183
SpeI ACTAGT 1 cut(s) 550
Sse9I AATT 7 cut(s) 272, 631, 756, 779, 1044, 1326, 1376
SseBI AGGCCT 2 cut(s) 518, 1210
SsiI CCGC 3 cut(s) 623, 1082, 1299
SspI AATATT 1 cut(s) 321
SspMI CTAG 5 cut(s) 38, 551, 800, 1022, 1556
StuI AGGCCT 2 cut(s) 518, 1210
StyD4I CCNGG 1 cut(s) 849
StyI CCWWGG 3 cut(s) 333, 744, 1068
TaaI ACNGT 3 cut(s) 370, 910, 1552
TaiI ACGT 2 cut(s) 1281, 1544
TaqI TCGA 1 cut(s) 391
TasI AATT 7 cut(s) 272, 631, 756, 779, 1044, 1326, 1376
TatI WGTACW 3 cut(s) 479, 819, 905
TfiI GAWTC 1 cut(s) 663
Tru1I TTAA 4 cut(s) 32, 876, 945, 1562
Tru9I TTAA 4 cut(s) 32, 876, 945, 1562
TscAI CASTG 5 cut(s) 699, 915, 1042, 1324, 1347
TseI GCWGC 4 cut(s) 200, 620, 1232, 1485
TspDTI ATGAA 3 cut(s) 908, 1488, 1507
TspGWI ACGGA 1 cut(s) 390
TspRI CASTG 5 cut(s) 699, 915, 1042, 1324, 1347
Van91I CCANNNNNTGG 1 cut(s) 51
VneI GTGCAC 1 cut(s) 1315
VpaK11BI GGWCC 1 cut(s) 1368
XapI RAATTY 1 cut(s) 1376
XspI CTAG 5 cut(s) 38, 551, 800, 1022, 1556
ZrmI AGTACT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.